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Geil, Ryan David. "Effects of exogenous ethylene on the formation of arbuscular mycorrhizae." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ56326.pdf.
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Smith, Caleb M. "Bioconcentration and Morphological Effects of Triclosan on Three Species of Wetland Plants." Thesis, University of North Texas, 2013. https://digital.library.unt.edu/ark:/67531/metadc271900/.
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Triclosan (TCS) is an antimicrobial compound found in several types of common household products. After being washed down the drain, TCS will then end up in the local watershed. Although numerous studies have been conducted to evaluate the fate and effects of TCS in aquatic environments, there have been no studies evaluating the role arbuscular mycorrhizal fungi (AM) play in a plants response to TCS exposure. Three species of wetland plants native North Texas were inoculated with AM spores and exposed to 0, 0.4 g/L and 4.0 g/L TCS concentrations. Root morphology of E. prostrata and S. herbacea showed AM and exposure responses. S. herbacea produced the greatest amounts biomass and TCS bioaccumulation, in all but one treatment. It also displayed opposing results to E. prostrata in measures of root length, root surface area, relative root mass, relative shoot mass and shoot:root ratio. TCS root tissue concentrations increased with increased exposures for both E. prostrata and S. herbacea. Even though E. prostrata had the lowest levels in each measure of biomass production, it had the highest amount of root TCS bioaccumulation in the AM inoculated 4.0 g/L treatment. H. laevis was between the other two species in terms of biomass production, and did not demonstrate any exposure or inoculation effects in morphology or TCS accumulation. Overall, no clear patterns were detected, which highlights the fact that further study is required to completely understand the effects compounds like TCS can have on plant community structure, and ultimately ecosystem function.
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Toljander, Jonas. "Interactions between soil bacteria and arbuscular mycorrhizal fungi /." Uppsala : Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, 2006. http://epsilon.slu.se/200639.pdf.
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Jenkins, Amy Miller. "Seed banking and vesicular-arbuscular mycorrhizae in pasture restoration in Central Florida." [Gainesville, Fla.] : University of Florida, 2003. http://purl.fcla.edu/fcla/etd/UFE0001399.
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Amerian, Mohammad Reza. "Effects of VA mycorrhizae and drought on the physiology of maize and bean grown singly and intercropped." Thesis, University of Newcastle Upon Tyne, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.247833.
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Seymour, Nicole Pamela. "Responses of linseed to vesicular-arbuscular mycorrhizae, phosphorus and zinc in a vertisol /." [St. Lucia, Qld.], 2002. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe17149.pdf.
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Sinclair, Grant. "Influence of Colonization by Arbuscular Mycorrhizal Fungi and a Root Endophyte on Selected Strawberry Cultivars Under Salt Conditions." Thèse, Université d'Ottawa / University of Ottawa, 2013. http://hdl.handle.net/10393/26124.
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Two factorial greenhouse experiments were performed to determine the effects of four arbuscular mycorrhizal fungi (AMF) species (Glomus arenarium, Funneliformis caledonius, F. mosseae, and Rhizophagus irregularis) and a root endophyte (Piriformospora indica) on four ‘day-neutral’ strawberry (Fragaria × ananassa Duch.) cultivars (‘Albion’, ‘Charlotte’, ‘Mara des Bois’, and ‘Seascape’), and mixed-AMF species (R. irregularis + F. mosseae) on cv. ‘Seascape’, under salt conditions (0-200 mM NaCl). In its biomass, ‘Seascape’ was more tolerant to salinity than the other cultivars. Cultivars responded differently to fungal inoculation as to salinity. G. arenarium had a negative effect on plant growth and ‘Mara des Bois’ responded negatively to inoculation. Among the remaining inoculants and cultivars, fungal-symbiosis was beneficial to growth. R. irregularis alleviated the symptoms of salt stress and improved fruit quality to a higher degree than the other AMF species and the root endophyte. Our results support the use of bio-inoculants in salty horticultural areas.
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Cooke, Margaret Anne. "Vesicular-arbuscular mycorrhizae and base cation fertilization in sugar maple (Acer saccharum marsh L.)." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=39428.
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Under field conditions, vesicles were the most frequently observed mycorrhizal structures in sugar maple, while greenhouse grown seedlings formed more arbuscules. Seasonal fluctuations of vesicular-arbuscular mycorrhizae existed. Mycorrhizal associations formed within 30 days in the greenhouse. Arbuscules were usually formed from hyphal coils and occasionally from linear hyphae spreading from cell to cell. Degenerating arbuscules were not observed. The addition of basic cations increased the number of vesicles formed and decreased the overall infection rates and seedling growth. The uptake of calcium, magnesium, and nitrogen decreased, and potassium uptake increased as fertilization rates increased. Positive correlations existed between the incidence of arbuscules and plant growth and health and between the incidence of arbuscules and the uptake of calcium, magnesium, nitrogen and phosphorus, and with the uptake ratios and these elements with potassium. This suggests that vesicular-arbuscular mycorrhizae may in some way be regulating ionic balance in these seedlings.
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Chaudhry, Tariq M., University of Western Sydney, and of Informatics Science and Technology Faculty. "Biogeochemical characterization of metalliferous wastes and potential role of arbuscular mycorrhizae in their phytoremediation." THESIS_FIST_XXX_Chaudhry_T.xml, 1999. http://handle.uws.edu.au:8081/1959.7/442.
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Phytoremediation is an innovative technology employing plants for reclamation of soils that have been polluted by industrial contaminants. While it is frequently slower than traditional physicochemical remediation techniques, there are, however, many significant advantages including simplicity, low cost, improvement of soil structure and microbiological fertility. While various hyperaccumulators have been reported from Europe and the USA, there is a need to identify Australian species. This study reports a number of metal accumulators following a soil and vegetation survey of two metal contaminated sites in New South Wales, the spoil (filtercake) heaps of the BHP (Broken Hill Propriety ) steelworks at Port Kembla and the abandoned mine at Sunny Corner. BHP is the largest manufacturer of steel in Australia. The Port Kembla is the largest single steel manufacturing site. Solid by-products from this site totalled 3.1 million tons in 1988. The Sunny Corner mine site was mainly a silver mine which operated over approximately 100 hectares 1184 to 1922 and produced over 100 tonnes of silver during that period. The soil and water in and around the site is heavily contaminated with metals and is distinctly acidic. It was concluded that Ricinus communis, Sonchus oleraceus, baeckea utilis, Poa labillardieri and Pinus radiata are options for heavy metal phytoremediation of contaminated Australian soils. Arbuscular Mycorrhizal infection (particular with Glomus and Gigaspora strains) should be promoted for optimum growth.Doctor of Philosophy (PhD)
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Chaudhry, Tariq M. "Biogeochemical characterization of metalliferous wastes and potential role of arbuscular mycorrhizae in their phytoremediation /." Campbelltown, N.S.W. : University of Western Sydney, Macarthur, Faculty of Informatics, Science and Technology, 1999. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030811.122338/index.html.
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Summers, William. "D14-LIKE : an essential protein for the establishment of arbuscular mycorrhizal symbiosis." Thesis, University of Cambridge, 2019. https://www.repository.cam.ac.uk/handle/1810/288351.
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Low nutrition availability in the soil can be a major limitation of plant growth. To improve nutrient acquisition, the majority of land plants engage in symbiosis with arbuscular mycorrhizal (AM) fungi. The accommodation of fungal colonisation structures in the roots requires their radical reprogramming. This starts during pre-symbiotic communication, where signals are exchanged between the fungus and plant across the rhizosphere. The receptor D14-LIKE emerged as a vital component of this pre-symbiotic communication when it was found to be absolutely required for symbiosis in rice. However, the broader relevance of the receptor, both in terms of functional conservation across plant species and its relation to other pre-symbiotic plant signalling components, remained unclear. The aim of this thesis was to elucidate these two key points. To address the fragmented picture of fungal signals, plant receptors and signalling pathways, a large scale transcriptomic experiment in rice was conducted to tie D14L together with other distinct pre-symbiotic components. In the absence of D14L-mediated signalling, rice was found to be compromised in the perception of germinated spore exudates, as well as specific chitinaceous signals, meaning that normal transcriptional reprogramming could not be achieved in response to any of these treatments. In addition, the functional conservation of D14L signalling was explored using trans-species complementation experiments. It was found that the Arabidopsis homolog AtKAI2 could complement the developmental phenotype of the d14l rice mutant, but not symbiosis. Likewise, D14La from early diverging Marchantia polymorpha and Marachantia paleacea could rescue developmental phenotypes in d14l rice, but again failed to complement symbiosis. This demonstrated a functional separation between developmental and symbiotic signalling. The data generated during my PhD foster D14L as a central node for multiple inputs to pre-symbiotic reprogramming, and provides new insights into pre-symbiotic communication mechanisms which are required for the successful establishment of symbiosis.
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Hossler, Katie. "Nutrient Cycling and the Role of Arbuscular Mycorrhizae in Created and Natural Wetlands of Central Ohio." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1275448235.
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Wan, Michael T. "The use of Glomus intraradices and vesicular-arbuscular mycorrhizae for assessing the environmental impact of azadirachtin." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/NQ37766.pdf.
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Gamiet, Sharmin. "Vesicular-arbuscular mycorrhizae and apples (Malus domestica Borkh.) in the nursery and in apple replant desease." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/27453.
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The purpose of this study was to determine if different clonal rootstock from apple (Malus domestica Borkh.) formed different vesicular arbuscular mycorrhizal (VAM) associations. Different fertilizers and VAM fungi were tested to determine their effects on apple seedling growth in apple replant diseased (ARD) soils. VAM associations in apple stoolbed nursery were low. Over 80% of all samples had less than 10% mycorrhizal colonization. This reduction in VAM colonization amongst various rootstock clones is a result of detrimental management practices in the stoolbed nursery. Apples grown in a budded nursery had high mycorrhizal colonization, the lowest colonization rate was 30%. Different rootstocks from the budded nursery do not show any significant differences in VAM colonization, whereas clonal rootstocks from the stoolbed nursery do. From the stoolbed nursery, Mailing (M) 2 consistantly showed higher VAM colonization rates, compared to M 4, M7, M9, M 26, Mailing Merton (MM) 106, MM 111, Alnarp 2 and Ottawa 3.
Apple replant disease (ARD) is identified as the reason for poor growth of apple seddlings in 5 soils from the Okanagan Valley of British Columbia. Sterilization by autoclaving, pasteurization and formalin increased test seedling height. Air-drying test soil does not affect ARD pot bioassays. However, air-drying the soil and pasteurizing or adding formalin increased plant height significantly more than these treatments in nonair-dried soils. The fertilizer monoammonium phosphate (11-55-0) increased plant height more than ammonium nitrate (34-0-0) while triple superphosphate (0-45-0) did not increase plant height. Root growth was increased by 0-45-0 only. VAM fungi were drastically reduced or eliminated by sterilization and 11-55-0, but not by the other fertilizers.
VAM fungi in 2 ARD soils do not overcome ARD. Test seedlings grown in sterilized ARD soils inoculated with 4 species of VAM fungi do not show as great an increase in shoot height compared to the addition of 11-55-0 fertilizer. Root growth shows the inverse response. Glomus intraradices Schenck and Smith, was the best colonizer but inoculation with G. versiforme (Karsten) Berch resulted in the greatest shoot and root growth. Glomus clarum Nicholson and Schenck, and G. monosporum Gerdemann and Trappe, did not result in increases in plant growth in ARD soils.
In sterilized ARD soils, VAM fungi do not increase shoot growth as expected, but do increase root growth, suggesting the initial growth of inoculated apple seedlings is root mass. Seedlings given 11-55-0 fertilizers show the reverse pattern of growth.
In nonsterilized ARD soils, the growth of seedlings appears to be inversely proportional to VAM colonization.Land and Food Systems, Faculty of
Graduate
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Wilkinson, Melinda M. "Changes in growth and survival by three co-occurring grass species in response to mycorrhizae, fire, and drought." Thesis, University of Hawaii at Manoa, 2003. http://proquest.umi.com/pqdweb?index=0&did=765961171&SrchMode=2&sid=6&Fmt=2&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1209417332&clientId=23440.
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Olsen, Margaret Anne. "How does dual-mycorrhizal association affect the ecological success of kanuka (Kunzea ericoides) across the South Island of New Zealand?" Thesis, University of Canterbury. School of Biological Sciences, 2015. http://hdl.handle.net/10092/10806.
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In this thesis I investigated the mutualism between Kunzea ericoides (kanuka) and two groups of soil fungi, ectomycorrhizae (ECM) and arbuscular mycorrhizae (AMF). Mycorrhizal mutualisms, which are considered globally ubiquitous, are poorly understood over changing abiotic gradients. A field survey of K. ericoides assessed how the relationship with the soil fungi varieties altered over a hydrologic gradient. Arbuscular mycorrhizal colonisation was significantly improved by increasing rainfall levels and amount of surrounding kanuka, and negatively affected by increasing altitude. Ectomycorrhizal colonisation was not significantly affected by any measured variables and remained relatively constant across all circumstances, suggesting that it is the preferred fungal mutualist for this tree species.
A glasshouse experiment was done to measure the effect of ECM inoculation on the growth and water usage of K. ericoides over varying moisture availability. The seedlings were planted in soil inoculated with both ECM and AMF (experimental) or AMF only (control) and then grown under varying levels of water stress. The experiment was replicated with two soil types, with soil from beneath adult manuka (Leptospermum scoparium) and soil from beneath adult kanuka (K. ericoides). ECM colonisation significantly increased as soil moisture decreased for both soil types. ECM inoculation also increased the root:shoot ratio, and drastically decreased water usage under drought conditions. There were some soil effects as the seedlings grown in manuka-soil achieving greater biomass than seedlings grown in kanuka-soil. This is possibly due to presence of pathogens or some type of legacy competition which the seedlings would experience growing near conspecifics.
Overall, K. ericoides formed a dominant mutualism with ectomycorrhizae. These two both thrive in dry environmental conditions and have a suite of complementing abilities which possibly allow K. ericoides to expand it range into these dry habitat types. The increased benefit of these mutualisms at the hydrologic range limit of the species supports the importance of biotic interaction mediating environmental stress. Understanding the effects and response of mycorrhizal mutualisms are especially significant considering current climate change issues in New Zealand and worldwide.
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Abu-Zeyad, Raeda, of Western Sydney Macarthur University, and Faculty of Business and Technology. "Occurrence of arbuscular mycorrhizae in castanospermum australe and their effect on growth and production of catanospermine (anti virus alkaloid)." THESIS_FBT_XXX_AbuZeyad_R.xml, 1997. http://handle.uws.edu.au:8081/1959.7/290.
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The present study was aimed to find out if there is any symbioses between C.australe roots and mycorrhizal fungi. This research also aimed to investigate the effect of Arbuscular mycorrhizal fungi on the growth of C.australe and the yield of alkaloid castanospermine. The rhizosphere soil and roots of C.australe from various sites in Sydney were collected. Roots were stained with vital and non-vital stains for assessment of mycorrhizal infection. The result indicated that AM fungi symbiotic associations with the roots of C.australe, producing arbuscules and vesicles in the root cortices. By wet sieving and decanting of rhizosphere soil, spores and sporocarps of AM fungi, were recovered. The spores mainly belonged to the genus Glomus. A correlation study was conducted to determine the relationship between the AM infection percentage in the roots and the Castanospermine amount in the leaves and seeds of the field grown trees. The results showed that there is a positive relationship between the castanospermine amount in the seeds and AM infection percentages in the roots. The effect of phosphorus on the yield of castanospermine was also investigated. The results indicated that phosphorus do enhance castanospermine at certain levels, but a further increase in phosphorus application resulted in reduced AM infection. It was found that AM has a great effect on the growth and production of C.australe and biosynthesis of castanospermine.Master of Science
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Mills, Mystyn W. "Bringing light to below ground patterns| Arbuscular mycorrhizae fungi diversity along an elevation gradient in Southern California." Thesis, California State University, Long Beach, 2015. http://pqdtopen.proquest.com/#viewpdf?dispub=1597781.
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Necessary for the diversity and survival of most terrestrial plants, arbuscular mycorrhizae (AMF) are fungi that form mutualistic symbiotic relationships with approximately 90 percent of terrestrial plant families. While the biodiversity and abundance of plants and animals have received much attention, these patterns for the belowground organisms on which they rely, such as AMF, remain poorly understood. While studies have found indications that AMF are fundamental to ecosystem structure and function, relatively few of these studies have been conducted in situ. In their ability to accommodate the complexity found in natural ecosystems, in situ studies may be vital in providing information relevant to the restoration and conservation of ecosystems. This thesis sought to explore in situ how AMF diversity and root colonization changed across ecosystems along an elevation gradient in Southern California. The findings indicate that certain soil parameters may be especially influential and that intra-species competition may play a role in AMF root colonization.
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Williams, Alwyn. "On the Ecology and Restoration of Podocarpus cunninghamii in the Eastern South Island High Country." Thesis, University of Canterbury. Forestry, 2010. http://hdl.handle.net/10092/5141.
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Podocarpus cunninghamii is an endemic New Zealand conifer that, in pre-human times, formed extensive forest communities across the eastern South Island high country. Anthropogenic disturbances have reduced the distribution of Podocarpus cunninghamii communities such that they now exist mainly as small and isolated remnants within a highly modified, predominantly pastoral landscape. Very little is known of the ecology of high country Podocarpus cunninghamii communities, and without this information it is not possible to develop an ecological basis for their restoration. This thesis explores the ecology of Podocarpus cunninghamii in the eastern South Island high country, investigating factors that potentially affect the restoration of Podocarpus cunninghamii within this environment, with special attention paid to the role of arbuscular mycorrhizal fungi (AMF).
Field investigations of Podocarpus cunninghamii communities showed that they contain a high degree of floristic and structural variation determined by soil and climatic variables. Analysis of age and size class distributions suggest that Podocarpus cunninghamii has more than one regeneration strategy, and can regenerate within intact forest following the opening of small canopy gaps or can undergo large-scale recruitment following catastrophic disturbance. Field and glasshouse experiments investigating growth and nutrient responses of Podocarpus cunninghamii to different AMF inoculants found that Podocarpus cunninghamii responses are dependent on both AMF type and grass competition. Finally, investigation of Podocarpus cunninghamii carbon stocks showed that they are less than that of other New Zealand forest types, but are greater than that of grazed pastures.
Successful restoration of high country Podocarpus cunninghamii communities will require the incorporation of associated species based on local environmental conditions, and will also need to allow for disturbance processes. AMF may have an important role to play in restoration by reducing seedling production times and by increasing the competitiveness of Podocarpus cunninghamii when in competition with exotic grasses.
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Cundiff, Gary Thomas. "Using Arbuscular Mycorrhizae to Influence Yield, Available Soil Nutrients and Soil Quality in Conventional VS. Organic Vegetable Production." TopSCHOLAR®, 2012. http://digitalcommons.wku.edu/theses/1155.
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This research is a two year study on the effects of endomycorrhizae on vegetable production using conventional vs. organic practices. Objective of this study was initiated to determine if mycorrhizae improve yield, available soil nutrients and soil quality from two different fertilizer sources. Measurements were taken on yield, available soil nutrients, and soil quality in comparison of glomalin production and soil loss percentage. Two plant species were chosen, Tomatoes (‘Big Beef’) and Bush Beans (‘Tenderette’). A randomized split block 2 x 3 factorial treatment arrangement was used with two crops and three different inputs: Mo- 0 mycorrhizae, M1- recommended rate, and M2- 2x recommended rate of mycorrhizae. Each mycorrhizal input was replicated three times in both the conventional and organic system. Results show there was no difference in yield based on mycorrhizae additions at any rate.
There was a significant yield difference based on conventional production over organic production in tomatoes and snap beans in 2010 and tomatoes in 2011. Possible explanations for yield difference in the organic production system include: different insect controls and a slower release of nutrients from poultry litter.
Available soil nutrients were not influenced in the study based on mycorrhizal inputs in inorganic or organic tomato production. Soil available nutrients were significantly influenced in organic tomato when compared to inorganic tomato production at selected sampling dates.
Mycorrhizae did not influence soil fertility in inorganic snap bean or organic snap bean production. Soil available nutrients were significantly influenced in organic snap bean when compared to inorganic snap bean production at selected sampling dates.
Glomalin production and soil loss percentage were not shown to be significantly different within organic or inorganic treatments based on mycorrhizae inputs. However, glomalin production was shown to be significantly greater in organic production compared to inorganic in 2011. An explanation of this could be due to the use of leaf mulch as organic weed control. Although a numerical decrease was observed in soil loss percentage in organic production compared to inorganic production from the first year to the second, it was not shown to be a significant amount.
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Bharadwaj, Dharam Parkash. "The plant - arbuscular mycorrhizal fungi - bacteria - pathogen system : multifunctional role of AMF spore-associated bacteria /." Uppsala : Dept, of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, 2007. http://epsilon.slu.se/200790.pdf.
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Santos-González, Juan Carlos. "Diversity of arbuscular mycorrhizal fungi in grasslands and arable fields : ecological factors related to community composition and dynamics /." Uppsala : Dept. of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, 2007. http://epsilon.slu.se/2007105.pdf.
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Joubert, Angelique. "Investigation on selected biotic and abiotic factors in the maintenance of the "fairy circles" (barren patches) of southern Africa." Diss., Electronic thesis, 2008. http://upetd.up.ac.za/thesis/available/etd-01172008-125625/.
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Artursson, Veronica. "Bacterial-fungal interactions highlighted using microbiomics : potential application for plant growth enhancement /." Uppsala : Dept. of Microbiology, Swedish University of Agricultural Sciences, 2005. http://epsilon.slu.se/2005127.pdf.
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Abu-Zeyad, Raeda. "Occurrence of arbuscular mycorrhizae in castanospermum australe and their effect on growth and production of catanospermine (anti virus alkaloid) /." [Campbelltown, N.S.W. : The Author], 1997. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030708.090926/index.html.
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Sangay-Tucto, Sheena. "Étude de l’impact des symbioses mycorhizienne et rhizobienne dans la domestication du Tara, Caesalpinia spinosa L." Thesis, Montpellier, 2018. http://www.theses.fr/2018MONTG080/document.
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La Tara (Caesalpinia spinosa) est une espèce forestière d’une grande importance en raison d’une forte demande sur le marché international pour les tanins présents dans ses gousses, et pour les gommes provenant de ses graines. Malgré son importance économique pour le Pérou, la majeure partie de la production provient de forêts naturelles non aménagées. Ces forêts présentent des problèmes de sol (érosion, faible fertilité, présence d’agents pathogènes, manque d'irrigation), qui conduisent à des rendements faibles. C’est pourquoi dans le présent travail, nous étudions les composantes microbiologiques du sol associé à cette culture, telles que les mycorhizes et les bactéries dont l’utilisation, selon de nombreuses études, s’est révélée être une alternative à l'utilisation d'engrais chimiques. Pour cela, nous avons procédé à l'analyse moléculaire de la diversité des champignons mycorhiziens arbusculaires par la technique de Miseq Illumina, ce qui nous a permis de mettre en évidence une prépondérance de Gloméracées parmi lesquelles les Rhizophagus spp. étaient retrouvés dans 70% des séquences. En outre, la dépendance de la Tara vis-à-vis de la mycorhization a été démontrée car, après avoir testé la mycorhization contrôlée de la Tara par Rhizophagus irregularis, il a été constaté que la croissance de Caesalpinia spinosa était considérablement améliorée, ainsi que l'absorption d'éléments nutritifs tels que l'azote (N) et le phosphore (P). Pour vérifier la capacité à noduler de la Tara, différents milieux de culture ont été utilisés ainsi que différentes conditions de croissance, en serre et in vitro. Ces expérimentations ont toutes montré que les racines de Tara ne présentaient pas de nodules, confirmant que cette légumineuse de la sous-famille des Caesalpinioideae est non nodulante. Par conséquent et afin d’étudier la diversité des rhizobia présents dans le sol de la plantation de Tara, nous avons utilisé en serre une plante-piège, le pois (Pisum sativum) car c’est une légumineuse nodulante et de plus est traditionnellement associée à la culture de Tara. Les rhizobia identifiés moléculairement se sont révélés très spécifiques et différents des rhizobia présents dans les sols extérieurs à la plantation de Tara. Plus particulièrement, ces rhizobia se sont révélés être phylogénétiquement proches de R. etli, R. phaseoli, R. pisi et R. leguminosarum. Enfin, un test d'inoculation contrôlée (in vitro) a été réalisé sur des plantules de pois, avec ces bactéries préalablement piégées et isolées du pois. Il a été observé que les rhizobia piégés à partir des sols collectés entre deux lignes de Tara et sur la ligne de plantation de Tara, ont stimulé la croissance du pois par rapport aux rhizobia présents dans les sols collectés à l'extérieur de la plantationThe Tara (Caesalpinia spinosa) is a forest species of great importance due to its high demand in the international market for the tannins present in its pods and its seeds’ gum. Despite its great importance for Peru, most of the production comes from unmanaged natural forests. These forests present soil problems (e.g., erosion, low fertility, pathogens, lack of irrigation), which cause low yields. Therefore, in the present work we seek to study the soil components associated with Tara plantation , such as mycorrhizae and bacteria that have proved to be an alternative for reducing the use of chemical fertilizers in similar context (Aboubacar et al., Flores Chavez 20015, E and Saif 1987, Dia et al. 2010; Bilgo et al., 2013) . We used molecular analysis of the arbuscular diversity by the Miseq Illumina technique that allowed to verify the arbuscular diversity with a preponderance of Glomeraceae among which the Rhizophagus spp were found to be present in 70% of the sequences. In addition, the dependence of the Tara on obligatory mycorrhization was demonstrated, after testing the controlled mycorrhization of the Tara by the Rhizophagus irregularis. We found that the growth of this crop was significantly improved, as well as the absorption of nutrients such as nitrogen (N) and phosphorus (P).To check the nodulation of the Tara, different culture media were used (JenSen, sand mixture with Tara plantation soil, attapulgite mixture with Tara plantation soils) in greenhouse and in vitro condition. We did not manage to find rhizobial nodules in the roots which let us think that Tara is a non-nodular legume. Therefore, we used Pisum sativum as a trap plant to study the diversity of rhizobia present in the soil of the Tara plantation since this legume is often associated with Tara crop. The rhizobia found in the trap plant were very specific and different from the rhizobia present in soils outside the Tara plantation. Likewise, these rhizobia found to be phylogenetically close to R. etli, R. phaseoli, R. pisi and R. leguminosarum. Finally, we inoculated the trapped bacterias (in vitro) in Pisum sativum with the bacterias previously trapped and isolated from the pea (which grew in the green house); where it was observed that the rhizospheric bacteria of the zones IL (soil collected between two lines) and L (soil collected from the same line) from the plantation of Tara stimulated the growth of this crop with respect to the bacteria present in soils collected outside of the plantation (OP zone)
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Asfaw, Zebene. "Tree species diversity, topsoil conditions and arbuscular mycorrhizal association in the Sidama traditional agroforestry land use, southern Ethiopia /." Uppsala : Dept. of Forest Management and Products, Swedish Univ. of Agricultural Sciences, 2003. http://epsilon.slu.se/s263-ab.html.
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Monteiro, Giselle Gomes. "Desenvolvimento de micorrizas arbusculares em mutantes hormonais de tomateiro (Lycopersicon esculentum cv Micro-Tom)." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-17032011-104351/.
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Os hormônios vegetais etileno e ABA são possíveis reguladores chave do desenvolvimento das micorrizas arbusculares (MAs). O primeiro objetivo deste trabalho foi avaliar o desenvolvimento da MA em mutantes superprodutores e insensíveis ao etileno (epinastic e Never ripe, respectivamente) e verificar se enzimas relacionadas ao sistema de defesa vegetal são reguladas diferencialmente pelo etileno durante o desenvolvimento da simbiose A colonização de raízes de epinastic (epi) por G. clarum foi significativamente reduzida quando comparada com Micro-Tom (MT), além disso, a funcionalidade da simbiose, demonstrada pelo acúmulo de transcritos de LePT4, foi fortemente reduzida quando comparada com MT. Por outro lado, o padrão de colonização e funcionalidade da simbiose no mutante Nr não diferiu de MT. Indução no acúmulo de transcritos de genes que codificam quitinases e enzimas relacionadas ao estresse oxidativo foi observada no mutante epi, enquanto o acúmulo de transcritos de uma -1,3-glucanase foi fortemente induzido no mutante Nr. O segundo objetivo foi verificar se a colonização reduzida observada em mutantes deficientes em ABA é dependente da produção do etileno. Para tanto, mutantes que diferem na deficiência de ABA e produzem diferentes quantidades de etileno (notabilis e sitiens) e o mutante epi foram inoculados com G. clarum e tratados com aminoethoxivinilglicina (AVG), um inibidor de etileno. A colonização intrarradicular de G. clarum nos mutantes epi e notabilis (not) foi significativamente reduzida quando comparada com MT, sendo que o mutante sitiens (sit) foi o menos susceptível à colonização em relação aos demais genótipos. A aplicação de AVG a 10 µM restaurou completamente a colonização em epi, mas não nos mutantes deficientes em ABA. O acúmulo de transcritos de LePT4 confirmou os resultados de colonização. Os genes que codificam enzimas chave na biossíntese do etileno, LeACS2 e LeACO4, foram regulados positivamente pela inoculação com G. clarum, e a restauração do desenvolvimento da MA observada em epi que recebeu AVG, provavelmente envolveu ACC oxidase (LeACO4). O acúmulo de transcritos dos genes que codificam ACC oxidase (LeACO1 e LeACO4) foi induzido significativamente em sit, mostrando que a superprodução de etileno observada neste mutante pode envolver a regulação da ACC oxidase. Por fim, indução no acúmulo de transcritos do gene LeNCED foi observada em todos os genótipos micorrizados e em raízes de epi que receberam AVG, demonstrando que LeNCED é regulado positivamente pela inoculação com G. clarum e que o etileno regula a expressão deste gene. Os resultados demonstram que etileno e ABA estão envolvidos na regulação do desenvolvimento de MA e que etileno modula a expressão de genes relacionados ao sistema de defesa vegetal permitindo o estabelecimento de uma simbiose funcional.The plant hormones ethylene and ABA are probably key developmental regulators of arbuscular mycorrhizae (AM). The first objective of this work was to evaluate the development of MA in overproducing and insensitive mutants to ethylene (epinastic and Never ripe, respectively) and verify if enzymes related to plant defense system are differentially regulated by ethylene during the symbiosis development. The colonization of epinastic (epi) roots by G. clarum was significantly reduced when compared with Micro-Tom (MT), moreover, the functionality of the symbiosis as demonstrated by transcripts accumulation of LePT4, was strongly reduced when compared with MT. Still, the pattern of colonization and functionality of the symbiosis in the Nr mutant did not differ from MT. Induction in the transcripts accumulation of genes encoding chitinase and enzymes related to oxidative stress was observed in epi mutant, whereas transcripts accumulation of -1, 3-glucanase was strongly induced in the Nr mutant. The second objective was to determine whether the reduced colonization observed in mutants deficient in ABA is dependent on ethylene production. For this purpose, mutants that differ in ABA deficiency and produce different amounts of ethylene (notabilis and sitiens) and the epi mutant were inoculated with G. clarum and treated with an ethylene inhibitor called aminoethoxivinilglicin (AVG). The intraradical colonization of G. clarum in epi and notabilis (not) mutants was significantly reduced when compared with MT and sitiens mutant was less susceptible to colonization in comparison with other genotypes. The application of AVG to 10 µM completely restored the colonization in the epi but not in mutants deficient in ABA. The transcripts accumulation of LePT4 confirmed the results of colonization. The genes that encode key enzymes in the biosynthesis of ethylene, LeACS2 and LeACO4, were positively regulated by inoculation with G. clarum and the restoration in the development of MA observed in epi which received AVG probably involved ACC oxidase (LeACO4). Transcripts accumulation of genes that encode ACC oxidase (LeACO1 and LeACO4) were induced significantly in sit showing that overproduction of ethylene in this mutant can involve ACC oxidase. Finally, the induction in the accumulation of gene transcripts LeNCED was observed in all genotypes and mycorrhizal roots of epi which received AVG demonstrating that LeNCED is regulated positively by the inoculation with G. clarum and that ethylene regulates the expression of this gene. Results show that ethylene and ABA are involved in regulating of MA development and ethylene modulates the expression of genes related to the plant defense system allowing the establishment and functionality of the symbiosis.
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Mortimer, Peter E. "The C-economy, nutritional benefits and symbiotic performance of dual inoculated Phaseolus vulgaris (L.) plants, under variable nutrient conditions." University of the Western Cape, 2010. http://hdl.handle.net/11394/8207.
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Philosophiae Doctor - PhDThe tripartite symbiosis between Phaseolus vulgaris, arbuscular-mycorrhiza and the nodule bacteria, Rhizobia have been the focus of many studies ranging over a number of decades, however these studies have failed to answer certain questions relating the role of the symbionts in regard to host nutrition and the subsequent influence of these symbionts on the host C- economy. There is little doubt over the synergistic benefits involved in the dual inoculation of bean plants, as well as the resultant C-costs of maintaining the 2 symbionts, yet the specific contribution of the individual symbionts to the hosts overall nutrient and C-economy remain to be clarified. Thus the aim of this thesis is to help clarify these points by determining the symbiont induced photosynthetic, respiratory and nutritional changes taking place in the host. This was achieved by a series of experiments in which nodulated bean plants were split into two categories-those with and without AM colonized roots. These plants were then exposed to a range of growing conditions, including hi and low P, and a series of N treatments, ranging from zero N through to 3 mM NH/. Under these differing nutrient conditions growth, photosynthetic, respiratory, nutrient and amino acid responses were monitored, thus allowing for the determination of the symbionts influence on the host and the hosts reliance on the respective symbionts. Host reliance was noted most strongly under nutrient limiting conditions. Under low P treatment AM was the dominant symbiont as far as host C was concerned, allowing for the early establishment of the AM, thus ensuring the uptake of P for both host and nodule development. High P affected AM colonization to a greater extent than it did nodule dry weight and conversely the addition of N~ + led to a greater decrease in nodule dry weight than it did AM colonization. In spite of this decline, AM benefited the host by improving host N nutrition and relieving N-feedback inhibition of the export amino acid asparagine on BNF. These AM induced benefits did come at a cost to the host though, the dual inoculated plants had higher below ground respiratory costs and subsequently higher photosynthetic rates to compensate for the increased demand for C. The higher photosynthetic rates associated with dual inoculation were as a result of symbiont induced sink stimulation and not due to the improved nutrition of the host, as shown by the photosynthetic and nutrient response ratios. However, the respiratory costs associated with the uptake of soil nutrients were lower in AM colonized roots, thus showing an increased efficiency in nutrient gain by AM colonized roots. This improvement in host N nutrition as a result of AM colonization, coupled with the lower respiratory costs of AM nutrition led to the conclusion that under certain growing conditions nodules can become redundant and possibly parasitic.
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Gao, Lingling. "Control of arbuscular mycorrhizal colonisation : studies of a mycorrhiza-defective tomato mutant." Title page, contents and summary only, 2002. http://web4.library.adelaide.edu.au/theses/09PH/09phg2117.pdf.
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Bibliography: leaves 161-178. This thesis characterises a mycorrhiza-defective tomato (Lycopersicon esculentum Mill.) mutant, rmc, with respect to fungal colonisation patterns and plant defence reactions during interactions with different species of arbuscular mycorrhizal (AM) fungi, root fungal pathogen Rhizoctonia solani and binucleate Rhizoctonia (a fungal parasite that colonises roots without causing disease). The results suggest that the mutated gene in rmc is involved in the regulation of recognition and plant defence responses in the establishment of AM symbioses.
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Ike-Izundu, Nnenna Esther. "Interaction between arbuscular mycorrhizal fungi and soil microbial populations in the rhizosphere." Thesis, Rhodes University, 2008. http://hdl.handle.net/10962/d1004021.
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This study examined the rehabilitation potential of AM fungi with organic and inorganic fertilisers under pot and field trial conditions as well as their interaction with rhizospheric organisms and specific functional groups. In addition, the study highlighted the effects of land-use management on AM fungal populations in soil and the mycorrhizal status of some selected plants from one of the study sites. The study focussed on two sites that differ in operational activities and these included a mined area that was to be rehabilitated and a commercial farming site. A pot trial was conducted using an overburdened soil resulting from kaolin clay mining. Pots were seeded with Cynodon dactylon and treated with either Organic Tea or NPK (3:1:5) fertiliser, with or without AM fungal inoculum. The compatibility of these fertilisers with AM fungi was assessed by plant growth and percentage root colonisation. Maximum shoot height and plant biomass were observed at the 28th week with NPK (3:1:5) fertiliser supporting mycorrhizal colonisation by 80%. The result indicated the potential of AM fungi to be used in rehabilitation with minimal phosphate fertiliser. Similarly, a field trial was set-up using 17 x 17 m[superscript 2] plots in the mining site that were treated with the same organic and inorganic fertilisers as well as with AM fungal inoculum in different combinations. The interaction between AM fungi and soil microbial population was determined using culture dependent and culture independent techniques. The culture dependent technique involved the use of soil dilution and plating on general purpose and selective media. The result showed that there was no change in the total culturable bacterial number in the untreated and AM fungal treated plots, while a change in species composition was observed in the functional groups. Different functional groups identified included nitrogen fixing bacteria, pseudomonads, actinomycetes, phosphate solubilisers and the fungal counterparts. Gram-positive bacteria were observed as the predominant phenotypic type, while nitrogen fixers and actinomycetes were the predominant functional groups. Species identified from each functional group were Pseudomonas fulva, Bacillus megaterium, Streptomyces and actinomycetales bacteria. Meanwhile, fungi such as Ampelomyces, Fusarium, Penicillium, Aspergillus, Cephalosporium and Exserohilium were identified morphologically and molecularly. Furthermore, the mining site had a significantly higher bacterial number than the farming site thereby indicating the effects of land-use management on culturable bacterial numbers. The culture independent technique was carried out by cloning of the bacterial 16S rDNA and sequencing. Identified clones were Bradyrhizobium, Propionibacterium and Sporichthya. A cladogram constructed with the nucleotides sequences of identified functional species, clones and closely related nucleotide sequences from the Genbank indicated that nucleotide sequences differed in terms of the method used. The activity and establishment of the introduced AM fungal population was determined by spore enumeration, infectivity assay, percentage root colonisation and assessment of glomalin concentrations. The results indicated that the two land use types affected AM fungal populations. However, the establishment of AM fungi in the farming site was more successful than in the mining site as indicated by the higher infectivity pontential. Selected host plants, which were collected around the mine area, were observed to be mainly colonised by AM fungi and these were identified as Pentzia incana, Elytropappus rhinocerotis, Euphorbia meloformis, Selago corymbosa, Albuca canadensis and Helichrysum rosum. These plant species were able to thrive under harsh environmental conditions, thereby indicating their potential use as rehabilitation host plants. Generally, the findings of this study has provided an insight into the interaction between arbuscular mycorrhizal fungi and other soil microorganisms in two fields with differing land use management practices.
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Filyaw, Tanner R. "Mycorrhizal Symbiosis in Forest-Grown American Ginseng (Panax quinquefolius), and the Effect of Mycorrhizal Colonization on Root Ginsenoside Concentrations." Ohio University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1500112365847568.
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Haller, Anjanette H. A. "The presence and role of arbuscular mycorrhizal fungi in coastal sand dune systems." Thesis, Rhodes University, 2000. http://hdl.handle.net/10962/d1003765.
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Arbuscular mycorrhizas (AM) are mutually beneficial symbiotic associations between the roots of plants and certain Zygomycetous fungi. The role of AM fungi in coastal sand dunes has been explored in many parts of the world, though little work has been conducted in South African dune systems. This study aimed to investigate the presence and extent of mycorrhizal colonisation of a coastal sand dune in South Africa. The roots of five plant species (Scaevola plumieri, Arctotheca populifolia, Ipomoea pes-caprae, Ehrharta villosa and Chrysanthemoides monilifera) were sampled along a foredune profile at Old Woman's River in the Eastern Cape. These roots were assessed for the percentage mycorrhizal colonisation they supported. Spores extracted from the rhizosphere sand of each plant species were counted and identified to genus level. Results were related to seasonality and the position of the plants along the profile. All plant species were found to be mycorrhizal. Percentage colonisation ranged from 0-92%, depending on plant species and season. Mycorrhizal colonisation was generally highest in the winter months, and especially so in I pes-caprae and E. villosa. The extent of various mycorrhizal structures in root tissue varied between plant species. Spore numbers ranged from 0-48 spores 100g-1 sand with highest numbers occurring in winter. S. plumieri and A. populifolia were associated with greatest spore abundance. Four fungal genera (Glomus, Acaulospora, Scutellospora and Gigaspora) were identified. Distribution of these genera showed seasonal variations between plant species. A bioassay, using Sorghum, was conducted to test the inoculum potentials of sand from the Scaevola hummock and the IpomoealEhrharta dune. Highest percentage colonisation occurred in plants grown in the Scaevola sand, which also had the lowest root and shoot measurements. The bioassay confirmed that AM propagules are present and viable, even in the mobile sand of the foredune. This study showed that mycorrhizal colonisation and spore numbers varied seasonally, but that the extent of this was dependent on plant species. The position of plants along the foredune profile tended to be less important than plant species. It is thought that the growth cycle and rooting system of each plant species determines seasonal cycles and abundance of AM fungi. Variation within fungal populations probably also impacts on this. Knowledge of the presence and distribution of AM fungi in this system paves the way for more detailed studies which need to examine the role of these endophytes in South African sand dunes.
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Thorne, Mark Ervin. "Calcareous Compacted Mine Soil in Southeast Ohio: A Prairie Grass Habitat." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1261263160.
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Muleta, Diriba. "Microbial inputs in coffee (Coffea arabica L.) production systems, southwestern Ethiopia : implications for promotion of biofertilizers and biocontrol agents /." Uppsala : Dept. of Microbiology, Swedish University of Agricultural Sciences, 2007. http://epsilon.slu.se/2007117.pdf.
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Cavagnaro, Timothy R. "Structure and physiology of Paris-type arbuscular mycorrhizas." Title page, contents and abstract only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phc376.pdf.
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Skinner, Amy. "Investigating the effect of Glomus etunicatum colonization on structure and phloem transport in roots of Eragrostis curvula (Umgeni)." Thesis, Rhodes University, 2007. http://hdl.handle.net/10962/d1003796.
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The symbiotic unit of an arbuscular mycorrhizal fungus and its host is able toachieve and maintain far higher inflow of nutrients than non-mycorrhizal roots. The colonization strategy of the mycobiont within the plant is intrinsic to the symbiosis with respect to both structural adaptations and nutrient exchange. An investigation into the effect of Glomus etunicatum colonization on the structure and phloem transport in Eragrostis curvula (Umgeni) allowed for greater insight into the dynamic of the symbiosis. The combined use of stains (such as Trypan Blue, Chlorazol Black, Safranin and Fast Green), and techniques, (such as freeze-microtome transverse sectioning and permanent slide preparations) contributed to a successful general observation of an intermediate colonization strategy using light microscopy methods. However, clarity into structural detail of mycorrhizal forms required electron microscopy studies. The SEM method used with freeze fracture was a relatively quick and simple method allowing for the observation of surface and internal features. The TEM method allowed for highresolution images providing insight into the variations in the apoplasmic compartmental form, and how this may relate to the function of the symbiosis with regard to fungal coils or arbuscules. The apoplasmic nature of mycorrhizas was substantiated and no symplasmic connections were found between symbionts. Fluorescence studies demonstrated that 5,6-carboxyfluorescein was transported through the phloem into the roots of E. curvula, but remained predominantly in the root phloem. Unloading only occurred in optimal nutrient exchange areas of meristimatic lateral or apical growth regions. It was not possible, using fluorescence techniques and related equipment available, to conclusively establish if there were symplasmic connections between the mycobiont and its host or if bidirectional transfer of nutrients occurred at the same interface.
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Zhang, Haoqiang. "Arbuscular mycorrhiza in Medicago truncatula." Doctoral thesis, Humboldt-Universität zu Berlin, Landwirtschaftlich-Gärtnerische Fakultät, 2014. http://dx.doi.org/10.18452/16925.
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Die arbuskuläre Mykorrhiza (AM) ist eine mutualistische Symbiose, die die Phosphataufnahme und Pathogenresistenz von Pflanzen verbessern kann. In der vorliegenden Doktorarbeit wurde die Rolle der Protonen-pumpenden ATPase MtHA1 für die AM Symbiose in Medicago truncatula untersucht. In MtHA1 Mutanten konnten AM Pilze nur noch verkürzte Arbuskel ohne typische Verzweigungen ausbilden. Dies zeigte sich auch in Expressionsmustern von Genen, die für Proteine in verschiedenen Bereichen der periarbuskulären Membran kodieren. Außerdem waren AM Pilzbesiedelung, die verbesserte Nährstoffaufnahme und die Wachstumsförderung in MtHA1 mutierten Pflanzen reduziert. Die Mykorrhiza-induzierte Resistenz (MIR) wurde näher in M. truncatula Pflanzen untersucht, die von Aphanomyces euteiches infiziert waren, dem Erreger einer Wurzelfäule in Leguminosen. In einem geteilten Wurzelsystem, das eine hohe Expression von Verteidigungsgenen aufwies, unterdrückte ein AM Pilz diese Expression und erhöhte in Folge die Empfindlichkeit für das Pathogen. In Wurzeln von Topfkulturen dagegen konnte eine typische MIR beobachtet werden, die wahrscheinlich auf erhöhter Aktivität der Jasmonat/Ethylen-regulierten Verteidigungsantwort beruht, verursacht durch eine Unterdrückung der Salizylsäuresynthese. Im Ergebnis zeigt diese Arbeit die bedeutende Rolle des Gens MtHA1 für die Bildung und Funktion der arbuskelhaltigen Zellen. Die Mutation des Gens führt zur verminderten Arbuskelverzweigung, reduzierter Phosphataufnahme und Wachstumsförderung in der Mykorrhiza und schließlich zu einer geringeren Gesamtbesiedelung durch den AM Pilz. Genexpressionsanalysen weisen darauf hin, dass unterschiedliche Mechanismen den lokalen und systemischen Wechselwirkungen zwischen AM Pilzen und Pathogenen in der Wurzel zu Grunde liegen. Verschieden physiologische Zustände von geteilten Wurzelsystemen und Wurzeln in Topfkulturen erschweren allerdings einen direkten Vergleich der beiden experimentellen Ansätze.Arbuscular mycorrhiza (AM) is a wide spread mutualistic symbiosis, which can improve phosphate acquisition and pathogen resistance of plants. In the current Ph.D. thesis the role of a proton pumping ATPase (MtHA1) for the AM symbiosis in Medicago truncatula was investigated. In MtHA1 mutant plants, different AM fungi only developed truncated arbuscules without forming typical hyphal branches, and this phenotype was mirrored by expression patterns of genes for proteins located in different areas of the periarbuscular membrane. AM fungal colonization, improved phosphate uptake and plant growth promotion were reduced in MtHA1 mutant plants. Mycorrhiza-induced resistance (MIR) and the nodule symbiosis were, however, not affected. MIR was further analyzed in the M. truncatula infected with Aphanomyces euteiches which causes a root-rot disease in legumes. In a split root system showing high levels of defense-gene expression, colonization of an AM fungus reduced this expression and in consequence increased susceptibility of the roots for the pathogen. In roots of pot cultures, however, a typical MIR was observed and could be based on the higher activity of jasmonate/ethylene-regulated defense responses due to suppression of salicylic acid biosynthesis. In conclusion, this work shows that the gene MtHA1 encoding a proton pumping-ATPase plays a critical role in the formation and function of arbuscule-containing cells. Expression of the mutated gene results in reduced formation of arbuscule branches. This in turn negatively influences mycorrhizal phosphate uptake, plant growth promotion and overall mycorrhizal colonization of the roots. Gene expression analyses indicate that different mechanisms underlay local and systemic interactions between the mycorrhizal fungus and the root pathogen. The different physiological stages of pot culture and split root system make a comparison of the two experimental approaches, however, difficult.
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Kasiamdari, Rina Sri. "Interactions between arbuscular mycorrhizal fungi and other root-infecting fungi." Title page, contents and abstract only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phk1887.pdf.
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Stockinger, Herbert. "DNA barcoding of arbuscular mycorrhizal fungi." Diss., lmu, 2010. http://nbn-resolving.de/urn:nbn:de:bvb:19-114870.
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Stewart, Lynda Irene. "Phosphorus effects on arbuscular mycorrhizal fungi." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=102729.
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Two field studies were conducted to assess the potential benefit of arbuscular mycorrhizal (AM) inoculation of elite strawberry plants on plant multiplication, and fruit yield, under typical nursery conditions, in particular soils classified as excessively rich in P. To study plant productivity, five commercially in vitro propagated elite strawberry cultivars ('Chambly', 'Glooscap', 'Joliette', 'Kent', and 'Sweet Charlie') were not inoculated with AM fungi or were inoculated with either a single species (Glomus intraradices), or a mixture of species (G. intraradices, Glomus mosseae, and Glomus etunicatum). AM inoculation was found to impact strawberry plant productivity in a soil with excessive P levels. The AM fungi introduced into the field by inoculated mother plants established a mycelial network in the soil through colonization of the daughter plant roots, however, persistence of colonization was determined to below (<12% in inoculated plant roots). In soils excessively rich in P, individual crop inoculation may be the only option for management of the symbiosis, as the host and non-host rotation crops, planted prior to strawberry production, had no effect on plant productivity or soil mycorrhizal potential.To study the impact of AM inoculation on fruit production, three commercially grown strawberry cultivars (Glooscap, Joliette, and Kent) were not inoculated with AM fungi or were inoculated with either G. intraradices or G. mosseae. AM fungi impacted the fruit yield, with all inoculated cultivars producing more fruit than noninoculated cultivars during the first harvest year. The percentage of root colonization could not be used to explain the differences in total fruit yield during the first harvest year, or the increase in total fruit yield the second harvest year.
We wished to examine the effects of various P treatments on C metabolism within the intraradical mycelia (IRM) of the fungus. Specific primers were developed for the Glomus intraradices glucose-6-phosphate dehydrogenase (G6PDH) gene. Real-time quantitative reverse transcriptase polymerase chain reaction (QRT-PCR) was used to measure the gene expression of the G. intrarardices G6PDH gene in response to external P conditions of colonized transformed carrot roots. The results showed a significant down-regulation of G6PDH in the IRM of G. intraradices when cultures were grown in a high P (350 muM P) medium compared to those grown in the low P (35 muM P) medium. The down-regulation may suggest a reduction in the C flow from the host to the fungus. There was no effect on G6PDH expression following a two-hour incubation with additional P applications (No P, low P and high P).
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Rewcastle, Joanne. "Plant protection using arbuscular mycorrhizal fungi." Thesis, University of Edinburgh, 2005. http://hdl.handle.net/1842/27261.
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The interaction between several species of arbuscular mycorrhizal fungi, micropropagated strawberry plants and Phytophthora fragariae, the pathogen that causes red stele disease of strawberry plants, was investigated. The optimum temperature for germination of zoospore cysts of P. fragariae in vitro was found to be 15°C, and growth of the emerging germ tube was significantly orientated towards the strawberry root tip. Cyst germination was reduced in the presence of a mycorrhizal strawberry root. Elsanta was more susceptible to P. fragariae than the cultivar Rhapsody. A low level of colonisation of Elsanta with the arbuscular mycorrhizal fungi Glomus mosseae, Glomus intraradices or Glomus fistulosum resulted in a significantly greater amount of total phosphorus in plant shoots compared to non-mycorrhizal plants, although further increases in the percentage of root colonisation by the fungi had no effect on the plants. The presence of these mycorrhizal fungi had no effect on disease due to subsequent inoculation of the plants by P. fragariae. Increasing colonisation of Elsanta by Scutellospora nodosa was correlated with a significant increase in plant size and additional phosphorus uptake. However, these same plants exhibited greater levels of disease due to the following inoculation with P. fragariae. A low level of root colonisation of Elsanta by Acaulospora scrobiculata caused significant increases in plant size and phosphorus uptake up to a threshold level of root colonisation beyond which further increases had no affect on the plant. The results are discussed in relation to the utilisation of specific strains of arbuscular mycorrhizal fungi as inoculants of micropropagated strawberry plants of particular cultivars with the potential to increase plant growth and reduce the level of disease due to soil-borne plant pathogens.
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Mathieu, Stephanie. "The Genetics of Arbuscular Mycorrhizal Fungi." Thesis, Université d'Ottawa / University of Ottawa, 2021. http://hdl.handle.net/10393/42770.
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Sexual reproduction is an important process amongst eukaryotic organisms, with one function being to maintain genetic variation. The idea that complex eukaryotic species can persist for millions of years in the absence of sex defies fundamental evolutionary dogma, yet a group of organisms known as ancient asexuals were thought to have evolved clonally under deep evolutionary time. Prominent among these are the arbuscular mycorrhizal fungi (AMF), which are obligate plant symbionts that colonize the root cells of plants and extend their hyphae into the soil assisting the plant in acquiring key nutrients. Unlike most eukaryotes, AMF cells are multinucleate with thousands of nuclei moving through a continuous cytoplasm. Genomic analyses have identified a putative mating-type (MAT) locus within the nuclear genomes of model AMF Rhizophagus irregularis, a region that in other fungi dictates the process of sexual reproduction. Additional findings demonstrated that AMF strains carry one of two nuclear organizations. They can be either homokaryotic (AMF homokaryons), where all nuclei within the cytoplasm are virtually identical, or heterokaryotic (AMF dikaryons), where two MAT-locus variants co-exist within the cytoplasm. Despite a lack of observable traits indicative of sex, this homo/heterokaryotic dichotomy is reminiscent of the nuclear organization of sexual fungi.
My research aims to build on these findings to investigate the actual role of the MAT-locus in driving AMF reproduction. To address this, I build my thesis into three main chapters. The first chapter reviews our current understanding of AMF genetics and what drives genome evolution in these organisms. The second chapter establishes a relatively easy, inexpensive, and reproducible approach to genotype known MAT variants of R. irregularis in natural and experimental conditions. The last chapter uses experimental crossings between strains to assess cytoplasmic compatibility and nuclear exchange. I demonstrate that dikaryotic spore progenies can be formed after co-culturing two distinct AMF homokaryotic strains. Further analyses of various genomic regions also reveal possible recombination in homokaryotic spore progenies from co-cultures. Overall, this research provides new experimental insights into the origin of genetic diversity in AMF. These findings open avenues to produce genetically new AMF strains in the lab using conventional crossing procedures and provide a glimpse of the mechanisms that generate AMF genetic diversity in the field.
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Lansing, Jennifer Lyn. "Comparing arbuscular and ectomycorrhizal fungal communities in seven North American forests and their response to nitrogen fertilization /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2003. http://uclibs.org/PID/11984.
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Thesis (Ph. D.)--University of California, Davis and San Diego State University, 2003.Includes bibliographical references (leaves 142-144). Also available via the World Wide Web. (Restricted to UC campuses).
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Facelli, Evelina. "The role of mycorrhizal symbiosis in plant intraspecific competition and population structure." Title page, Contents and Abstract only, 1998. http://hdl.handle.net/2440/37773.
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The overall objective of this project was to investigate the effects of the symbiotic association of plants with vesicular - arbuscular mycorrhizal fungi on the intensity of intraspecific competition and its consequences on population structure I performed four main glasshouse experiments using a non - cultivated species, Rhodanthe chlorocephala ssp rosea, or a cultivated species, Trifolium subterraneum. I grew the plants at different plant densities, under different levels of resources ( phosphorus and / or light ), in environments with homogeneous and / or patchy distribution of phosphorus ( P ). In pots with homogeneous distribution of P, the addition of P to R. chlorocephala and mycorrhizal infection in T. subterraneum increased plant biomass of single plants. However, these beneficial effects were reduced by increasing plant density. Shading of plants of T. subterraneum did not generally alter these effects. Mycorrhizal symbiosis and the addition of P always increased the intensity of plant intraspecific competition. In trays with patchy or homogeneous distribution of P, mycorrhizal infection and patchy distribution of P increased the total biomass and size inequality of populations of plants of T. subterraneum. Individual biomass was determined by the local soil P concentration in patchy environments and by mycorrhizal infection in low density treatments. Mycorrhizal infection, but not patchy P distribution, increased relative competition intensity. Asymmetric or symmetric distribution of resources between plants will change these size hierarchies. The distinction between these two types of distributions has lead to two different models explaining the interaction between competition and size inequality ( degree to which the biomass is concentrated within a small fraction of the population &# 40 Weiner and Thomas 1986 ) ) the resource depletion and resource pre - emption models ( Weiner and Thomas 1986, Weiner 1988b ). In the first model ( resource depletion ) competition reduces the relative growth rate of all the individuals by the same proportion, reduces variance of growth rates and reduces variation in sizes. Thus, in this model resource acquisition is proportional to plant size ( Weiner 1990 ). This model is also called symmetric or two - sided competition and applies when competition for nutrients predominates. It predicts that at high density, plants will be smaller but the population will have less inequality than at low density ( Weiner and Thomas 1986 ). In the second model ( resource pre - emption ), competition increases the variation in relative growth rates and increases variation in sizes. Large plants obtain a more than proportional share of the resources ( relative to sizes ) ( Weiner 1990 ) and this increases their competitive ability which results in a positive feedback on plant size. This phenomenon is also called snowball cumulation, asymmetric or one - sided competition and it was observed only when competition for light was predominant ( Wilson 1988a ). This second model predicts that at high density plant populations will have more inequality than at low density ( Weiner and Thomas 1986 ). Although these two models are generally accepted, alternative analyses and recent experiments show that the degree of asymmetry of the interaction depends on the spatial and temporal distribution of the resource, the spatial distribution of the individuals in the population, neighbourhood competition and the mobility of the resource ( Huston 1986 ; Miller and Weiner 1989, Weiner 1990, Bonan 1991 ). Weiner ( 1990 ) suggested that if nutrients are distributed homogeneously and the uptake is proportional to root size, the competitive interaction will be more symmetric, whereas if patches with more nutrients can be reached by large individuals, asymmetric competition will predominate. This hypothesis has not been tested yet. Turner and Rabinowitz ( 1983 ) found that populations with an initial random spatial distribution of individuals had an unexpected increase in size inequality. My results emphasise that the main effects of mycorrhizas at the individual level cannot be expected to be apparent at the population level, because of the influence of density - dependent processes. However, infected individuals with a strong response to the symbiosis would have an advantage in situations of competition. This scenario can explain the maintenance of the symbiotic ability even under conditions such as dense populations, where there is no obvious advantage of the symbiosis at the population level.Thesis (Ph.D.)--Department of Soil and Water, 1998.
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Pivato, Barbara. "Ecology of arbuscular mycorrhizas : interactions plant - fungal genotypes and mycorrhizas - bacteria." Dijon, 2008. http://www.theses.fr/2008DIJOS006.
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La première étape de la thèse a consisté à comparer la diversité et la structure génétique des populations de champignons mycorhiziens à arbuscules (MA) associées à quatre espèces de médiques annuelles. Les résultats obtenus montrent que l’abondance des champignons MA différait, indiquant que la structure génétique de la communauté fongique a été influencée par l’espèce végétale. La deuxième étape de ce travail visait à tester l’hypothèse selon laquelle la longue histoire évolutive entre champignons MA et plantes ne se serait pas faite de façon indépendante des bactéries. La structure génétique des populations bactériennes associées aux racines mycorhizées et non de M. Truncatula a été comparée. Les communautés bactériennes différaient de façon significative, ces différences étant expliquées par des marqueurs moléculaires associés aux familles des Oxalobacteraceae et Comamonadaceae. Six isolats représentatifs des populations associées aux racines mycorhizées et deux souches de référence (Collimonas fungivorans Ter331 et Pseudomonas fluorescens C7R12) ont été testées afin de déterminer leur effet sur la mycorhization. Une souche appartenant aux Oxalobacteraceae (Collimonas sp. J5B4) et P. Fluorescens C7R12 ont amélioré la croissance du champignon AM et sa colonisation racinaire, confirmant partiellement l’hypothèse que les bactéries associées aux racines mycorhizées auraient un effet favorable sur la mycorhization. Enfin, la caractérisation de l’effet promoteur de P. Fluorescens C7R12 sur la mycorhization a montré que cet effet était spécifique du champignon MA et que la colonisation des cellules bactériennes différait sur les racines mycorhizées et non-mycorhizéesIn the first part of the thesis, possible effect of the plant genotype on the genetic diversity and structure of the arbuscular mycorrhizal (AM) fungal community was assessed. Results indicated a similar diversity of AM fungi in the four Medicago species used. However, the abundance of AM fungi differed significantly upon the plant species, indicating preferential associations between AM fungal and plant genotypes. The second part of the thesis was based on the hypothesis that the long joint evolution of AM fungi and plants did not occur independently of the associated bacteria. To test this hypothesis, the genetic structure of bacterial communities associated with mycorrhizal and non mycorrhizal roots of M. Truncatula was compared. The bacterial communities differed significantly between mycorrhizal and non mycorrhizal roots, these differences being explained by sequences belonging to Oxalobacteraceae and Comamonadaceae families. Six bacterial isolates representative of the populations belonging to Oxalobacteraceae and Comamonadaceae were tested together with two reference strains (Collimonas fungivorans Ter331 and Pseudomonas fluorescens C7R12) for their effect on mycorrhization. One strain belonging to Oxalobacteraceae (Collimonas sp. J5B4) and P. Fluorescens C7R12 promoted both AM fungal growth and mycorrhization, partially confirming our hypothesis that bacteria preferentially associated with mycorrhizal roots would be beneficial to the symbiosis. Finally, the characterization of the promoting effect of P. Fluorescens C7R12 showed that it was fungal specific and that the pattern of colonization of bacterial cells on mycorrhizal and non mycorrhizal roots differed
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Duensing, Nina. "Transport processes in the arbuscular mycorrhizal symbiosis." Phd thesis, Universität Potsdam, 2013. http://opus.kobv.de/ubp/volltexte/2013/6821/.
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The nutrient exchange between plant and fungus is the key element of the arbuscular mycorrhizal (AM) symbiosis. The fungus improves the plant’s uptake of mineral nutrients, mainly phosphate, and water, while the plant provides the fungus with photosynthetically assimilated carbohydrates. Still, the knowledge about the mechanisms of the nutrient exchange between the symbiotic partners is very limited. Therefore, transport processes of both, the plant and the fungal partner, are investigated in this study. In order to enhance the understanding of the molecular basis underlying this tight interaction between the roots of Medicago truncatula and the AM fungus Rhizophagus irregularis, genes involved in transport processes of both symbiotic partners are analysed here.
The AM-specific regulation and cell-specific expression of potential transporter genes of M. truncatula that were found to be specifically regulated in arbuscule-containing cells and in non-arbusculated cells of mycorrhizal roots was confirmed. A model for the carbon allocation in mycorrhizal roots is suggested, in which carbohydrates are mobilized in non-arbusculated cells and symplastically provided to the arbuscule-containing cells. New insights into the mechanisms of the carbohydrate allocation were gained by the analysis of hexose/H+ symporter MtHxt1 which is regulated in distinct cells of mycorrhizal roots. Metabolite profiling of leaves and roots of a knock-out mutant, hxt1, showed that it indeed does have an impact on the carbohydrate balance in the course of the symbiosis throughout the whole plant, and on the interaction with the fungal partner. The primary metabolite profile of M. truncatula was shown to be altered significantly in response to mycorrhizal colonization.
Additionally, molecular mechanisms determining the progress of the interaction in the fungal partner of the AM symbiosis were investigated. The R. irregularis transcriptome in planta and in extraradical tissues gave new insight into genes that are differentially expressed in these two fungal tissues. Over 3200 fungal transcripts with a significantly altered expression level in laser capture microdissection-collected arbuscules compared to extraradical tissues were identified. Among them, six previously unknown specifically regulated potential transporter genes were found. These are likely to play a role in the nutrient exchange between plant and fungus. While the substrates of three potential MFS transporters are as yet unknown, two potential sugar transporters are might play a role in the carbohydrate flow towards the fungal partner.
In summary, this study provides new insights into transport processes between plant and fungus in the course of the AM symbiosis, analysing M. truncatula on the transcript and metabolite level, and provides a dataset of the R. irregularis transcriptome in planta, providing a high amount of new information for future works.In der arbuskulären Mykorrhiza (AM) Symbiose werden die Wurzeln fast aller Landpflanzen von Pilzen der Abteilung Glomeromycota besiedelt. Der Pilz erleichtert der Pflanze die Aufnahme von Mineralien, hauptsächlich Phosphat, und Wasser. Im Gegenzug versorgt die Pflanze ihn mit Photoassimilaten. Trotz der zentralen Bedeutung der Austauschmechanismen zwischen Pilz und Pflanze ist nur wenig darüber bekannt. Um die molekularen Grundlagen der Interaktion zwischen den Wurzeln der Leguminose Medicago truncatula und dem arbuskulären Mykorrhizapilz Rhizophagus irregularis besser zu verstehen, werden hier die Transportprozesse, die zwischen den Symbiosepartnern ablaufen, näher untersucht. Die zellspezifische Regulation der Transkription potentieller M. truncatula Transporter Gene in arbuskelhaltigen und nicht-arbuskelhaltigen Zellen mykorrhizierter Wurzeln wird bestätigt. Ein Modell zur möglichen Verteilung von Kohlenhydraten in mykorrhizierten Wurzeln, nach dem Zucker in nicht-arbuskelhaltigen Zellen mobilisiert und symplastisch an arbuskelhaltige Zellen abgegeben werden, wird vorgestellt. Die Analyse eines Mykorrhiza-induzierten Hexose/H+ Symporter Gens, MtHxt1, liefert neue Einsichten in die Mechanismen der Kohlenhydratverteilung in mykorrhizierten Pflanzen. Metabolitanalysen von Wurzeln und Blättern einer knock-out Mutante dieses Gens zeigen dessen Einfluss auf den Kohlenhydrathaushalt der ganzen Pflanze und auf die Interaktion mit dem Pilz. Die Metabolitzusammensetzung von M. truncatula wird durch die Mykorrhiza Symbiose signifikant beeinflusst. Darüber hinaus werden durch Transkriptomanalysen die molekularen Grundlagen der AM Symbiose auf der Seite des Pilzes analysiert. Arbuskeln wurden mittels Laser Capture Mikrodissektion direkt aus mykorrhizierten Wurzeln isoliert. Über 3200 pilzliche Transkripte weisen in diesen Arbuskeln im Vergleich zu extraradikalen Geweben ein deutlich verändertes Expressionslevel auf. Unter diesen Transkripten sind auch sechs zuvor unbekannte Gene, die für potentielle Transporter codieren und mit großer Wahrscheinlichkeit eine Rolle im Nährstoffaustausch zwischen Pilz und Pflanze spielen. Während die Substrate von drei potentiellen MFS Transportern noch unbekannt sind, spielen zwei potentiellen Zuckertransporter möglicherweise eine Rolle im Transport von Kohlenhydraten in Richtung des Pilzes. Zusammengefasst bietet diese Arbeit neue Einsichten in Transportprozesse zwischen Pilz und Pflanze im Laufe der AM Symbiose. M. truncatula Transkript- und Metabolitlevel werden analysiert und die Transkriptomanalyse von R. irregularis liefert einen umfassenden Datensatz mit einer großen Menge an Informationen zu der noch unzureichend erforschten pilzlichen Seite der Symbiose für folgende Arbeiten.
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Olsen, Erla. "Temperature responses of the arbuscular mycorrhizal symbiosis." Thesis, University of York, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.440981.
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Davidson, Kent. "Genetic studies of vesicular-arbuscular mycorrhizal fungi." Thesis, University of Bristol, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.279742.
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Kang, Hyun-Joo. "The basic genetics of arbuscular mycorrhizal fungi." [Bloomington, Ind.] : Indiana University, 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3358927.
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Thesis (Ph.D.)--Indiana University, Dept. of Biology, 2009.Title from PDF t.p. (viewed on Feb. 8, 2010). Source: Dissertation Abstracts International, Volume: 70-05, Section: B, page: 2653. Adviser: James D. Bever.