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1

Saldaña, Miguel A., Shivanand Hegde, and Grant L. Hughes. "Microbial control of arthropod-borne disease." Memórias do Instituto Oswaldo Cruz 112, no. 2 (February 2017): 81–93. http://dx.doi.org/10.1590/0074-02760160373.

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2

Denman, Susan, and Ann Marie Hart. "Arthropod-borne Disease: West Nile Fever." Journal for Nurse Practitioners 11, no. 1 (January 2015): 27–33. http://dx.doi.org/10.1016/j.nurpra.2014.10.017.

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3

Mairuhu, A. T. A., J. Wagenaar, D. P. M. Brandjes, and E. C. M. van Gorp. "Dengue: an arthropod-borne disease of global importance." European Journal of Clinical Microbiology & Infectious Diseases 23, no. 6 (June 1, 2004): 425–33. http://dx.doi.org/10.1007/s10096-004-1145-1.

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4

Qian, Zhuolei Jolly. "Biosafety Aspects of Arthropod-Borne Disease Vectors Laboratory." Applied Biosafety 22, no. 3 (June 9, 2017): 92–96. http://dx.doi.org/10.1177/1535676017713208.

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5

SPARAGANO, O. A. E. "Impact of ticks and tick-borne diseases on agriculture and human populations in Europe." Journal of Agricultural Science 143, no. 6 (September 20, 2005): 463–68. http://dx.doi.org/10.1017/s0021859605005526.

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Ticks are considered in Europe to be the most important arthropod group responsible for vector-borne diseases in humans, while in the tropics mosquitoes take over this position with ticks being the second most important. Over the last decade, vector-borne diseases have proliferated within Southern Europe (Blue tongue and West Nile viruses, both mosquito-borne diseases) while human ehrlichiosis (a tick-borne disease) has increased dramatically in Eastern Europe.
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6

Cavrini, Francesca, Paolo Gaibani, Anna Maria Pierro, Giada Rossini, Maria Paola Landini, and Vittorio Sambri. "Chikungunya: an emerging and spreading arthropod-borne viral disease." Journal of Infection in Developing Countries 3, no. 10 (November 21, 2009): 744–52. http://dx.doi.org/10.3855/jidc.169.

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The infection caused by the virus Chikungunya is known since the last 50 years, but since the disease was mainly diffuse in geographical areas located in developing countries, a few research work have been made available until the appearance of an important epidemiological outbreak in 2005 in the island of La Reunion, that is part of metropolitan France even if located in the Southern Eastern part of the Indian Ocean. In 2007, a smaller outbreak of Chikungunya developed in the Northern Eastern part of Italy, where the local transmission has been made possible by the enormous population of Aedes albopictus and the presence of a viremic patient coming from the Indian Ocean area. Nowadays, Chikungunya is spreading in Southeast Asia countries, including Indonesia, Malaysia, Thailand and Singapore. This paper reviews different aspects of the disease caused by Chikungunya virus, including: history, epidemiology, biological and pathogenetic aspects, clinical pictures, diagnosis and treatment.
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7

Benelli, Giovanni, Riccardo Petrelli, and Angelo Canale. "Arthropod-Borne Disease Control at a Glance: What’s New on Drug Development?" Molecules 25, no. 21 (November 6, 2020): 5175. http://dx.doi.org/10.3390/molecules25215175.

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Discovering and validating effective drugs to manage arthropod-borne diseases (ABD) is a timely and important research challenge with major impacts on real-world control programs at the time of quick resistance development in the targeted pathogens. This editorial highlights major research advances in the development of drugs for the control of vector-borne diseases, with a significant focus on malaria, Chagas disease, dengue, human African trypanosomiasis, leishmaniasis, and Zika. Broad reviews providing new insights on ABD recently published in Molecules have also been covered in “The Editors’ pick” section.
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8

Melanson, Vanessa R., Jessica L. Scheirer, Marshall T. Van de Wyngaerde, Kevin Bourzac, Shuenn-Jue Wu, Tadeusz Kochel, and James C. McAvin. "Leveraging Arthropod-Borne Disease Surveillance Assays for Clinical Diagnostic Use." Military Medicine 179, no. 11 (November 2014): 1207–11. http://dx.doi.org/10.7205/milmed-d-14-00019.

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9

Stanek, G., G. Wewalka, V. Groh, R. Neumann, and W. Kristoferitsch. "DIFFERENCES BETWEEN LYME DISEASE AND EUROPEAN ARTHROPOD-BORNE BORRELIA INFECTIONS." Lancet 325, no. 8425 (February 1985): 401. http://dx.doi.org/10.1016/s0140-6736(85)91424-2.

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10

Tabachnick, Walter J. "Evolutionary Genetics and Arthropod-borne Disease: The Yellow Fever Mosquito." American Entomologist 37, no. 1 (1991): 14–26. http://dx.doi.org/10.1093/ae/37.1.14.

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11

Jeffries, C. L., K. L. Mansfield, L. P. Phipps, P. R. Wakeley, R. Mearns, A. Schock, S. Bell, A. C. Breed, A. R. Fooks, and N. Johnson. "Louping ill virus: an endemic tick-borne disease of Great Britain." Journal of General Virology 95, no. 5 (May 1, 2014): 1005–14. http://dx.doi.org/10.1099/vir.0.062356-0.

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In Europe and Asia, Ixodid ticks transmit tick-borne encephalitis virus (TBEV), a flavivirus that causes severe encephalitis in humans but appears to show no virulence for livestock and wildlife. In the British Isles, where TBEV is absent, a closely related tick-borne flavivirus, named louping ill virus (LIV), is present. However, unlike TBEV, LIV causes a febrile illness in sheep, cattle, grouse and some other species, that can progress to fatal encephalitis. The disease is detected predominantly in animals from upland areas of the UK and Ireland. This distribution is closely associated with the presence of its arthropod vector, the hard tick Ixodes ricinus. The virus is a positive-strand RNA virus belonging to the genus Flavivirus, exhibiting a high degree of genetic homology to TBEV and other mammalian tick-borne viruses. In addition to causing acute encephalomyelitis in sheep, other mammals and some avian species, the virus is recognized as a zoonotic agent with occasional reports of seropositive individuals, particularly those whose occupation involves contact with sheep. Preventative vaccination in sheep is effective although there is no treatment for disease. Surveillance for LIV in Great Britain is limited despite an increased awareness of emerging arthropod-borne diseases and potential changes in distribution and epidemiology. This review provides an overview of LIV and highlights areas where further effort is needed to control this disease.
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12

Titus, R. G., and J. M. C. Ribeiro. "The role of vector saliva in transmission of arthropod-borne disease." Parasitology Today 6, no. 5 (May 1990): 157–60. http://dx.doi.org/10.1016/0169-4758(90)90338-5.

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13

Young, G. David, and Sandra Evans. "Safety and Efficacy of DEET and Permethrin in the Prevention of Arthropod Attack." Military Medicine 163, no. 5 (May 1, 1998): 324–30. http://dx.doi.org/10.1093/milmed/163.5.324.

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Abstract Many preventable diseases affecting troop strength are directly attributed to disease-carrying insects. The first line of defense against arthropod vectors is the use of personal protective measures. The concurrent application of DEET (N,N-diethyl-m-toluamide) repellent on the skin and permethrin [(3-phenoxy-phenyl)methyl(±)cis,trans-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropane-carboxylate] insecticide on the battle dress uniform, while the uniform is worn properly, is a personal protective strategy officially known as the DOD Insect Repellent System. It is important for troop commanders and field leaders to enforce the use of personal protective measures to prevent insect-borne infectious diseases and to ensure troop and soldier readiness. DEET is a safe and effective repellent. Permethrin is a synthetic pyrethroid insecticide and repellent. Used in conjunction with proper clothing and other personal protective equipment, these repellents provide the best known protection available and are critical in minimizing the occupational health threat of arthropod-borne diseases to troops in the field.
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14

Blair, Carol D., Zachary N. Adelman, and Ken E. Olson. "Molecular Strategies for Interrupting Arthropod-Borne Virus Transmission by Mosquitoes." Clinical Microbiology Reviews 13, no. 4 (October 1, 2000): 651–61. http://dx.doi.org/10.1128/cmr.13.4.651.

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SUMMARY Arthropod-borne virus (arbovirus) infections cause a number of emerging and resurgent human and veterinary infectious diseases. Traditional means of controlling arbovirus diseases include vaccination of susceptible vertebrates and mosquito control, but in many cases these have been unavailable or ineffective, and so novel strategies for disease control are needed. One possibility is genetic manipulation of mosquito vectors to render them unable to transmit arboviruses. This review describes recent work to test the concept of pathogen-derived resistance in arthropods by expression of viral genes in mosquito cell cultures and mosquitoes. Sense and antisense genome sequences from La Crosse virus (LAC) (a member of the Bunyaviridae) and dengue viruses serotypes 1 to 4 (DEN-1 to DEN-4) (members of the Flaviviridae) were expressed in mosquito cells from double-subgenomic and replicon vectors based on Sindbis virus (a member of the Togaviridae). The cells were then challenged with homologous or related viruses. For LAC, expression of antisense sequences from the small (S) genome segment, particularly full-length antisense S RNA, effectively interfered with replication of challenge virus, whereas expression of either antisense or sense RNA from the medium (M) segment was completely ineffective in LAC inhibition. Expression of sense and antisense RNA derived from certain regions of the DEN genome also blocked homologous virus replication more effectively than did RNA from other regions. Other parameters of RNA-mediated interference have been defined, such as the time when replication is blocked and the minimum size of effector RNA. The mechanism of RNA inhibition has not been determined, although it resembles double-stranded RNA interference in other nonvertebrate systems. Prospects for application of molecular strategies to control arbovirus diseases are briefly reviewed.
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15

André, Marcos Rogério, Kilder Dantas Filgueira, Ana Cláudia Calchi, Keyla Carstens Marques de Sousa, Luiz Ricardo Gonçalves, Vitor Brasil Medeiros, Poliana Araújo Ximenes, Ivana Cristina Nunes Gadelha Lelis, Maria Vanuza Nunes de Meireles, and Rosangela Zacarias Machado. "Co-infection with arthropod-borne pathogens in domestic cats." Revista Brasileira de Parasitologia Veterinária 26, no. 4 (November 9, 2017): 525–31. http://dx.doi.org/10.1590/s1984-29612017064.

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Abstract The role of several feline vector-borne pathogens (FVBP) as a cause of disease in cats has not been clearly determined. In fact, with the exception of Bartonella spp. and hemoplasmas, FVBP in cats has not been clearly determined in Brazil yet. The present study aimed at identifying, by using molecular methods, the presence of FVBP in three cats showing non-specific clinical signs and inclusions suggestive of hemoparasites in blood smears. Cytauxzoon felis, ‘Candidatus Mycoplasma haemominutum’, Ehrlichia sp. closely related to Ehrlichia canis, and Anaplasma sp. closely related to Anaplasma phagocytophilum were detected in blood samples from two out of three sampled cats. Both cats positive for multiple FVBP did not show hematological and biochemical abnormalities. The present work emphasizes the need for molecular confirmation of co-infection by multiple FVBP in cats presenting non-specific clinical signs and inclusions resembling hemoparasites in blood smears.
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16

Artsob, Harvey. "Arthropod-borne disease in Canada: A clinician's perspective from the ‘Cold Zone’." Paediatrics & Child Health 5, no. 4 (May 1, 2000): 206–12. http://dx.doi.org/10.1093/pch/5.4.206.

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17

Hughes, G. L., and J. L. Rasgon. "Transinfection: a method to investigateWolbachia-host interactions and control arthropod-borne disease." Insect Molecular Biology 23, no. 2 (December 11, 2013): 141–51. http://dx.doi.org/10.1111/imb.12066.

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18

BOCK, R., L. JACKSON, A. DE VOS, and W. JORGENSEN. "Babesiosis of cattle." Parasitology 129, S1 (October 2004): S247—S269. http://dx.doi.org/10.1017/s0031182004005190.

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Tick fever or cattle fever (babesiosis) is economically the most important arthropod-borne disease of cattle worldwide with vast areas of Australia, Africa, South and Central America and the United States continuously under threat. Tick fever was the first disease for which transmission by an arthropod to a mammal was implicated at the turn of the twentieth century and is the first disease to be eradicated from a continent (North America). This review describes the biology ofBabesiaspp. in the host and the tick, the scale of the problem to the cattle industry, the various components of control programmes, epidemiology, pathogenesis, immunity, vaccination and future research. The emphasis is onBabesia bovisandBabesia bigemina.
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19

O'Brien, Caitlin A., Roy A. Hall, and Ala Lew-Tabor. "Could Australian ticks harbour emerging viral pathogens?" Microbiology Australia 39, no. 4 (2018): 185. http://dx.doi.org/10.1071/ma18060.

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Tick-borne viruses contribute significantly to the disease burden in Europe, Asia and the US. Historically, some of the most well-known viruses from this group include the human pathogens, tick-borne encephalitis virus and Crimean-Congo haemorrhagic fever virus. More recently multiple emerging tick-borne viruses have been associated with severe disease in humans with Bourbon virus and Heartland virus isolated from patients in the US and severe fever with thrombocytopenia syndrome virus reported from China, Japan, and South Korea. Such examples highlight the need for broader approaches to survey arthropod pathogens, to encompass not only known but novel pathogens circulating in Australian tick populations.
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20

Antony, Suresh. "Mosquito and Tick-borne Illnesses in the United States. Guidelines for the Recognition and Empiric Treatment of Zoonotic Diseases in the Wilderness." Infectious Disorders - Drug Targets 19, no. 3 (October 4, 2019): 238–57. http://dx.doi.org/10.2174/1871526518666180626123340.

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Background:In the United States, tick-borne illnesses account for a significant number of patients that have been seen and treated by health care facilities. This in turn, has resulted in a significant morbidity and mortality and economic costs to the country.Methods:The distribution of these illnesses is geographically variable and is related to the climate as well. Many of these illnesses can be diagnosed and treated successfully, if recognized and started on appropriate antimicrobial therapy early in the disease process. Patient with illnesses such as Lyme disease, Wet Nile illness can result in chronic debilitating diseases if not recognized early and treated.Conclusion:This paper covers illnesses such as Lyme disease, West Nile illness, Rocky Mountain Spotted fever, Ehrlichia, Tularemia, typhus, mosquito borne illnesses such as enteroviruses, arboviruses as well as arthropod and rodent borne virus infections as well. It covers the epidemiology, clinical features and diagnostic tools needed to make the diagnosis and treat these patients as well.
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21

Taus, Naomi S., Colette Cywes-Bentley, Wendell C. Johnson, Gerald B. Pier, Lindsay M. Fry, Michelle R. Mousel, and Massaro W. Ueti. "Immunization against a Conserved Surface Polysaccharide Stimulates Bovine Antibodies with Opsonic Killing Activity but Does Not Protect against Babesia bovis Challenge." Pathogens 10, no. 12 (December 9, 2021): 1598. http://dx.doi.org/10.3390/pathogens10121598.

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Arthropod-borne apicomplexan pathogens remain a great concern and challenge for disease control in animals and humans. In order to prevent Babesia infection, the discovery of antigens that elicit protective immunity is essential to establish approaches to stop disease dissemination. In this study, we determined that poly-N-acetylglucosamine (PNAG) is conserved among tick-borne pathogens including B. bovis, B. bigemina, B. divergens, B. microti, and Babesia WA1. Calves immunized with synthetic ß-(1→6)-linked glucosamine oligosaccharides conjugated to tetanus toxoid (5GlcNH2-TT) developed antibodies with in vitro opsonophagocytic activity against Staphylococcus aureus. Sera from immunized calves reacted to B. bovis. These results suggest strong immune responses against PNAG. However, 5GlcNH2-TT-immunized bovines challenged with B. bovis developed acute babesiosis with the cytoadhesion of infected erythrocytes to brain capillary vessels. While this antigen elicited antibodies that did not prevent disease, we are continuing to explore other antigens that may mitigate these vector-borne diseases for the cattle industry.
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22

Poh, Karen C., Jesse R. Evans, Michael J. Skvarla, and Erika T. Machtinger. "All for One Health and One Health for All: Considerations for Successful Citizen Science Projects Conducting Vector Surveillance from Animal Hosts." Insects 13, no. 6 (May 24, 2022): 492. http://dx.doi.org/10.3390/insects13060492.

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Many vector-borne diseases that affect humans are zoonotic, often involving some animal host amplifying the pathogen and infecting an arthropod vector, followed by pathogen spillover into the human population via the bite of the infected vector. As urbanization, globalization, travel, and trade continue to increase, so does the risk posed by vector-borne diseases and spillover events. With the introduction of new vectors and potential pathogens as well as range expansions of native vectors, it is vital to conduct vector and vector-borne disease surveillance. Traditional surveillance methods can be time-consuming and labor-intensive, especially when surveillance involves sampling from animals. In order to monitor for potential vector-borne disease threats, researchers have turned to the public to help with data collection. To address vector-borne disease and animal conservation needs, we conducted a literature review of studies from the United States and Canada utilizing citizen science efforts to collect arthropods of public health and veterinary interest from animals. We identified common stakeholder groups, the types of surveillance that are common with each group, and the literature gaps on understudied vectors and populations. From this review, we synthesized considerations for future research projects involving citizen scientist collection of arthropods that affect humans and animals.
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23

Manning, Jessica E., and Tineke Cantaert. "Time to Micromanage the Pathogen-Host-Vector Interface: Considerations for Vaccine Development." Vaccines 7, no. 1 (January 21, 2019): 10. http://dx.doi.org/10.3390/vaccines7010010.

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The current increase in vector-borne disease worldwide necessitates novel approaches to vaccine development targeted to pathogens delivered by blood-feeding arthropod vectors into the host skin. A concept that is gaining traction in recent years is the contribution of the vector or vector-derived components, like salivary proteins, to host-pathogen interactions. Indeed, the triad of vector-host-pathogen interactions in the skin microenvironment can influence host innate and adaptive responses alike, providing an advantage to the pathogen to establish infection. A better understanding of this “bite site” microenvironment, along with how host and vector local microbiomes immunomodulate responses to pathogens, is required for future vaccines for vector-borne diseases. Microneedle administration of such vaccines may more closely mimic vector deposition of pathogen and saliva into the skin with the added benefit of near painless vaccine delivery. Focusing on the ‘micro’–from microenvironments to microbiomes to microneedles–may yield an improved generation of vector-borne disease vaccines in today’s increasingly complex world.
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24

Hassan, Mohammad Sayeed, Sheikh Farjana Sonia, Mohammad Abdullah Yusuf, Ferdous Ara, Ahmad Raihan Sharif, Farid Ahmed, and Md Atiqur Rahman. "Chikungunya Patient presented with Arthritis: A Case Report." Journal of Current and Advance Medical Research 1, no. 1 (January 1, 2014): 21–22. http://dx.doi.org/10.3329/jcamr.v1i1.19562.

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Chikungunya is an arthropod borne viral disease considered as the new emerging infectious disease in many countries of Asia, Africa, Europe and America. In this present case report a 45 year old lady was presented with multiple joint pains following exanthematous febrile illness. Laboratory result revealed this case as positive for chikungunya.DOI: http://dx.doi.org/10.3329/jcamr.v1i1.19562J Curr Adv Med Res 2014;1(1):21-22
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25

Marzal, Alfonso, Sergio Magallanes, and Luz Garcia-Longoria. "Stimuli Followed by Avian Malaria Vectors in Host-Seeking Behaviour." Biology 11, no. 5 (May 9, 2022): 726. http://dx.doi.org/10.3390/biology11050726.

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Vector-borne infectious diseases (e.g., malaria, dengue fever, and yellow fever) result from a parasite transmitted to humans and other animals by blood-feeding arthropods. They are major contributors to the global disease burden, as they account for nearly a fifth of all infectious diseases worldwide. The interaction between vectors and their hosts plays a key role driving vector-borne disease transmission. Therefore, identifying factors governing host selection by blood-feeding insects is essential to understand the transmission dynamics of vector-borne diseases. Here, we review published information on the physical and chemical stimuli (acoustic, visual, olfactory, moisture and thermal cues) used by mosquitoes and other haemosporidian vectors to detect their vertebrate hosts. We mainly focus on studies on avian malaria and related haemosporidian parasites since this animal model has historically provided important advances in our understanding on ecological and evolutionary process ruling vector-borne disease dynamics and transmission. We also present relevant studies analysing the capacity of feather and skin symbiotic bacteria in the production of volatile compounds with vector attractant properties. Furthermore, we review the role of uropygial secretions and symbiotic bacteria in bird–insect vector interactions. In addition, we present investigations examining the alterations induced by haemosporidian parasites on their arthropod vector and vertebrate host to enhance parasite transmission. Finally, we propose future lines of research for designing successful vector control strategies and for infectious disease management.
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26

Temeyer, Kevin B., Kristie G. Schlechte, Pia U. Olafson, Barbara S. Drolet, Jason P. Tidwell, Weste L. A. Osbrink, Allan T. Showler, Aaron D. Gross, and Adalberto A. Pérez de León. "Association of Salivary Cholinesterase With Arthropod Vectors of Disease." Journal of Medical Entomology 57, no. 6 (May 27, 2020): 1679–85. http://dx.doi.org/10.1093/jme/tjaa096.

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Abstract Acetylcholinesterase (AChE) was previously reported to be present in saliva of the southern cattle tick, Rhipicephalus (Boophilus) microplus (Canestrini), with proposed potential functions to 1) reduce acetylcholine toxicity during rapid engorgement, 2) modulate host immune responses, and 3) to influence pathogen transmission and establishment in the host. Potential modulation of host immune responses might include participation in salivary-assisted transmission and establishment of pathogens in the host as has been reported for a number of arthropod vector-borne diseases. If the hypothesis that tick salivary AChE may alter host immune responses is correct, we reasoned that similar cholinesterase activities might be present in saliva of additional arthropod vectors. Here, we report the presence of AChE-like activity in the saliva of southern cattle ticks, Rhipicephalus (Boophilus) microplus; the lone star tick, Amblyomma americanum (Linnaeus); Asian tiger mosquitoes, Aedes albopictus (Skuse); sand flies, Phlebotomus papatasi (Scopoli); and biting midges, Culicoides sonorensis Wirth and Jones. Salivary AChE-like activity was not detected for horn flies Haematobia irritans (L.), stable flies Stomoxys calcitrans (L.), and house flies Musca domestica L. Salivary cholinesterase (ChE) activities of arthropod vectors of disease-causing agents exhibited various Michaelis–Menten KM values that were each lower than the KM value of bovine serum AChE. A lower KM value is indicative of higher affinity for substrate and is consistent with a hypothesized role in localized depletion of host tissue acetylcholine potentially modulating host immune responses at the arthropod bite site that may favor ectoparasite blood-feeding and alter host defensive responses against pathogen transmission and establishment.
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27

Umar, H. A., M. F. Abdul Khanan, D. A. Umar, M. S. Shiru, M. Isma'il, M. R. Mohd Salleh, A. Abdul Rahman, A. Ahmad, and E. A. Okoli. "MAPPING POTENTIAL HABITATS FOR ARTHROPOD VECTORS OF TRYPANOSOMIASIS INFECTION IN NORTHERN NIGERIA: AN INTRODUCTORY SYNTHESIS." ISPRS - International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences XLII-4/W9 (October 30, 2018): 147–52. http://dx.doi.org/10.5194/isprs-archives-xlii-4-w9-147-2018.

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<p><strong>Abstract.</strong> This paper presents an introductory synthesis for mapping potential habitats of arthropod vectors responsible for animal trypanosomiasis diseases in Northern Nigeria, where there is high production of livestock. Animal trypanosomiasis is considered an arthropod-borne viral disease which is endemic in 36 countries of sub-Saharan Africa and particularly in northern Nigeria. The disease which is transmitted by the vector tsetse fly remains a threat to both humans and livestock in many rural communities of Nigeria. The outbreak of the disease is known to occur as a result of the changing climate which relates to changes in sea surface temperatures otherwise known as “El Niño Southern Oscillations” (ENSO). Trypanosomiasis is mainly experienced whenever there are changes in global precipitation as a result of the changing climate. Monthly Satellite data of Normalized Difference Vegetation Index (NDVI) at 2.5&amp;deg; spatial resolution was sourced from NASA-MODIS/CMD and subjected to principal component analysis using standardized principal components of GIS with a digital elevation model (DEM) supplemented in the analysis. Results revealed pockets of probable habitats of arthropod vectors to be around forest islands characterized by dry woodland and savanna, and in other cases around gallery forests and few lowland and riverine areas. This study demonstrates that geospatial technology is a cost effective tool in mapping of the arthropod vector habitats for Northern Nigeria.</p>
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28

Zakham, Fathiah, Aishah E. Albalawi, Abdullah D. Alanazi, Phuoc Truong Nguyen, Abdulaziz S. Alouffi, Altaf Alaoui, Tarja Sironen, Teemu Smura, and Olli Vapalahti. "Viral RNA Metagenomics of Hyalomma Ticks Collected from Dromedary Camels in Makkah Province, Saudi Arabia." Viruses 13, no. 7 (July 18, 2021): 1396. http://dx.doi.org/10.3390/v13071396.

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Arthropod-borne infections are a medical and economic threat to humans and livestock. Over the last three decades, several unprecedented viral outbreaks have been recorded in the Western part of the Arabian Peninsula. However, little is known about the circulation and diversity of arthropod-borne viruses in this region. To prepare for new outbreaks of vector-borne diseases, it is important to detect which viruses circulate in each vector population. In this study, we used a metagenomics approach to characterize the RNA virome of ticks infesting dromedary camels (Camelus dromedaries) in Makkah province, Saudi Arabia. Two hundred ticks of species Hyalomma dromedarii (n = 196) and Hyalomma impeltatum (n = 4) were collected from the Alkhurma district in Jeddah and Al-Taif city. Virome analysis showed the presence of several tick-specific viruses and tick-borne viruses associated with severe illness in humans. Some were identified for the first time in the Arabian Peninsula. The human disease-associated viruses detected included Crimean Congo Hemorrhagic fever virus and Tamdy virus (family Nairoviridae), Guertu virus (family Phenuiviridae), and a novel coltivirus that shares similarities with Tarumizu virus, Tai forest reovirus and Kundal virus (family Reoviridae). Furthermore, Alkhurma hemorrhagic virus (Flaviviridae) was detected in two tick pools by specific qPCR. In addition, tick-specific viruses in families Phenuiviridae (phleboviruses), Iflaviridae, Chuviridae, Totiviridae and Flaviviridae (Pestivirus) were detected. The presence of human pathogenetic viruses warrants further efforts in tick surveillance, xenosurveillence, vector control, and sero-epidemiological investigations in human and animal populations to predict, contain and mitigate future outbreaks in the region.
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29

Harikrishnan, Elackiya. "Fulminant Myocarditis: A Very Rare Manifestation of Dengue Fever." Journal of Communicable Diseases 52, no. 02 (June 30, 2022): 99–102. http://dx.doi.org/10.24321/0019.5138.202278.

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Dengue is a highly prevalent arthropod-borne viral disease in tropical and subtropical areas of the globe. The majority of dengue cases are benign but rare complications and presentations are seen increasingly due to the rising burden of the disease mainly in tropical countries. In dengue, clinical manifestations vary from asymptomatic infection to severe disease. Dengue fever is characterised by fever, myalgia, arthralgia, headache and bleeding manifestations. We report a rare case of dengue fever complicated with acute myocarditis. The patient recovered gradually with supportive care.
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Mylonaki, Eirini, Michael Seiberl, Neil Jones, Heike Bernhard, Ferdinand Otto, Georg Pilz, Eugen Trinka, and Peter Wipfler. "Tick-Borne Encephalitis Virus RNA Found in Frozen Goat’s Milk in a Family Outbreak." International Journal of Molecular Sciences 23, no. 19 (October 1, 2022): 11632. http://dx.doi.org/10.3390/ijms231911632.

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Tick-borne encephalitis (TBE) is one of the commonest arthropod-borne viral diseases in Middle-East Europe and North Asia. The main reservoir of the virus is comprised of small rodents and domestic mammals with the common tick (Ixodes) being the usual vector. The clinical spectrum of TBE ranges from mild meningitis to severe meningoencephalomyelitis. This disease can lead to severe sequelae and has a mortality up to 2% in Europe. Even though the majority of cases are transmitted through bites of infected ticks, infections through ingestion of contaminated milk and dairy products from farms in endemic areas have been reported. We report a family outbreak of a febrile disease, initially suggestive of human-to-human infection, during the early summertime in Austria. Tick-borne encephalitis was diagnosed following consumption of unpasteurised goat’s milk and the virus was subsequently detected in frozen milk samples. Although this is a rare manifestation of TBE, this case series shows that TBE should be included in the differential diagnosis of an outbreak of febrile disease, and a careful clinical history with reference to unpasteurized dairy products is crucial in order to prevent further disease spread. The best preventive measure is active immunisation of people living in, or travelling to, endemic areas.
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Shareef, Adnan, Ahmad Mursel Anam, Zahirul Islam, and Raihan Rabbani. "Dengue Fever induced Myocarditis." Bangladesh Critical Care Journal 4, no. 2 (October 21, 2016): 125–27. http://dx.doi.org/10.3329/bccj.v4i2.30030.

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Dengue is an arthropod-borne viral illness, mostly asymptomatic, but may present with a wide variety of clinical manifestations, from mild febrile illness to severe and fatal disease. Cardiac involvement is a significant yet under-reported one because of lack of awareness. We report a case of myocarditis in Dengue Fever with an intention to create awareness among the physicians.Bangladesh Crit Care J September 2016; 4 (2): 125-127
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Durand, Jonas, Maxime Jacquet, Lye Paillard, Olivier Rais, Lise Gern, and Maarten J. Voordouw. "Cross-Immunity and Community Structure of a Multiple-Strain Pathogen in the Tick Vector." Applied and Environmental Microbiology 81, no. 22 (August 28, 2015): 7740–52. http://dx.doi.org/10.1128/aem.02296-15.

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ABSTRACTMany vector-borne pathogens consist of multiple strains that circulate in both the vertebrate host and the arthropod vector. Characterization of the community of pathogen strains in the arthropod vector is therefore important for understanding the epidemiology of mixed vector-borne infections.Borrelia afzeliiandB. gariniiare two species of tick-borne bacteria that cause Lyme disease in humans. These two sympatric pathogens use the same tick,Ixodes ricinus, but are adapted to different classes of vertebrate hosts. BothBorreliaspecies consist of multiple strains that are classified using the highly polymorphicospCgene. Vertebrate cross-immunity against the OspC antigen is predicted to structure the community of multiple-strainBorreliapathogens.Borreliaisolates were cultured from field-collectedI. ricinusticks over a period spanning 11 years. TheBorreliaspecies of each isolate was identified using a reverse line blot (RLB) assay. Deep sequencing was used to characterize theospCcommunities of 190B. afzeliiisolates and 193B. gariniiisolates. Infections with multipleospCstrains were common in ticks, but vertebrate cross-immunity did not influence the strain structure in the tick vector. The pattern of genetic variation at theospClocus suggested that vertebrate cross-immunity exerts strong selection against intermediately divergentospCalleles. Deep sequencing found that more than 50% of our isolates contained exoticospCalleles derived from otherBorreliaspecies. Two alternative explanations for these exoticospCalleles are cryptic coinfections that were not detected by the RLB assay or horizontal transfer of theospCgene betweenBorreliaspecies.
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Hernandez, Raquel, Dennis T. Brown, and Angel Paredes. "Structural Differences Observed in Arboviruses of the Alphavirus and Flavivirus Genera." Advances in Virology 2014 (2014): 1–24. http://dx.doi.org/10.1155/2014/259382.

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Arthropod borne viruses have developed a complex life cycle adapted to alternate between insect and vertebrate hosts. These arthropod-borne viruses belong mainly to the families Togaviridae, Flaviviridae, and Bunyaviridae. This group of viruses contains many pathogens that cause febrile, hemorrhagic, and encephalitic disease or arthritic symptoms which can be persistent. It has been appreciated for many years that these viruses were evolutionarily adapted to function in the highly divergent cellular environments of both insect and mammalian phyla. These viruses are hybrid in nature, containing viral-encoded RNA and proteins which are glycosylated by the host and encapsulate viral nucleocapsids in the context of a host-derived membrane. From a structural perspective, these virus particles are macromolecular machines adapted in design to assemble into a packaging and delivery system for the virus genome and, only when associated with the conditions appropriate for a productive infection, to disassemble and deliver the RNA cargo. It was initially assumed that the structures of the virus from both hosts were equivalent. New evidence that alphaviruses and flaviviruses can exist in more than one conformation postenvelopment will be discussed in this review. The data are limited but should refocus the field of structural biology on the metastable nature of these viruses.
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McGready, Rose, Elizabeth A. Ashley, Vanaporn Wuthiekanun, Saw Oo Tan, Mupawjay Pimanpanarak, Samuel Jacher Viladpai-nguen, Wilarat Jesadapanpong, et al. "Arthropod Borne Disease: The Leading Cause of Fever in Pregnancy on the Thai-Burmese Border." PLoS Neglected Tropical Diseases 4, no. 11 (November 16, 2010): e888. http://dx.doi.org/10.1371/journal.pntd.0000888.

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KENT, REBEKAH J. "Molecular methods for arthropod bloodmeal identification and applications to ecological and vector-borne disease studies." Molecular Ecology Resources 9, no. 1 (January 2009): 4–18. http://dx.doi.org/10.1111/j.1755-0998.2008.02469.x.

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36

Azar, Sasha R., Rafael K. Campos, Nicholas A. Bergren, Vidyleison N. Camargos, and Shannan L. Rossi. "Epidemic Alphaviruses: Ecology, Emergence and Outbreaks." Microorganisms 8, no. 8 (August 1, 2020): 1167. http://dx.doi.org/10.3390/microorganisms8081167.

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Over the past century, the emergence/reemergence of arthropod-borne zoonotic agents has been a growing public health concern. In particular, agents from the genus Alphavirus pose a significant risk to both animal and human health. Human alphaviral disease presents with either arthritogenic or encephalitic manifestations and is associated with significant morbidity and/or mortality. Unfortunately, there are presently no vaccines or antiviral measures approved for human use. The present review examines the ecology, epidemiology, disease, past outbreaks, and potential to cause contemporary outbreaks for several alphavirus pathogens.
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Saito, Tais B., and David H. Walker. "Eosinophils in tick transmitted ehrlichial infection." Journal of Immunology 202, no. 1_Supplement (May 1, 2019): 190.58. http://dx.doi.org/10.4049/jimmunol.202.supp.190.58.

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Abstract Vector-borne diseases pose significant global health concerns with tick-borne disease doubling over the past 13 years. Tick-borne ehrlichiosis are life-threatening diseases that affect humans and animals. Many studies have been focused on the pathogenesis of these infections; however not much is known about in vivo immune responses to tick transmitted ehrlichiosis. With our model of a human pathogen Ehrlichia (EML) transmitted by ticks, we observed intense changes at the site of infection that persisted for several days. Lethal infection suggests an involvement of vascular dysfunction in disease pathogenesis. Therefore, the goal of this study was to evaluate the granulocyte response to tick transmission, primarily eosinophils. Skin site of the arthropod attachment showed intense infiltration of granulocytes, extending through dermal and subcutaneous tissue. The predominant cells contained eosinophilic granules and were surrounded by other poly and mono-nuclear cells. Also, we further identified increased cellular signaling associated with granulocyte recruitment and vascular dysfunction. The progression of disease was also characterized by increased eosinophils in the blood and immune mediators (e.g. IL-1β) in serum, indicative of proliferation, chemotaxis, and activation of these cells. A needle infection model demonstrated that granulocytes were implicated in immunopathology, bacterial replication, over-production of cytokines/chemokines. We believe that tick transmitted ehrlichiosis induces eosinophilic inflammation that result in release of an arsenal of mediators that regulate inflammation and vascular permeability and could be involved in the establishment of infection and/or disease development.
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38

Karim, Mst Tahmina, Hamida Khanum, and Sharmin Musa. "Communicable Diseases Among the Female Inhabitants of Lower Socio-Economic Groups in Dhaka City." Bangladesh Journal of Zoology 48, no. 1 (June 29, 2020): 105–17. http://dx.doi.org/10.3329/bjz.v48i1.47880.

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A total of 900 female inhabitants of lower socioeconomic in Dhaka city, were examined during September, 2013 to August, 2015. Among them, the prevalence of gastrointestinal parasitic infestation was 66.22%. The prevalence of Urinary tract infection (UTI) was 31.44%. Among the female inhabitants, 41.33% were infected with different types of skin diseases; within these, viral, (19.35%) scabies and 38.09% were affected by boil disease. The prevalence of vector-borne diseases was infected and arthropod infections (9.41%) were found. Among them, the highest 54.28% were infected by scabies and 38.09% were affected by boil diseases. The prevalence of vector-borne diseases was 49.33%, among them four types of diseases were recorded of which 2% malaria, 7.33% filaria, 34.67% dengue and 5.33%. leishmaniasis. Four types of waterborne diseases were found such as cholera/ diarrhoea (28%), typhoid (17.11%), polio (5.11%) and hepatitis A/ Jaundice (9.67%); and overall prevalence was 59.87%. Regarding the airborne diseases, 58.44% were found to be infected, among them four types recorded, such as influenza (25.11%), mumps (10.22%), pneumonia (17.44%) and tuberculosis (5.67%). Bangladesh J. Zool. 48(1): 105-117, 2020
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39

Olajiga, Olayinka, Andrés F. Holguin-Rocha, Meagan Rippee-Brooks, Megan Eppler, Shanice L. Harris, and Berlin Londono-Renteria. "Vertebrate Responses against Arthropod Salivary Proteins and Their Therapeutic Potential." Vaccines 9, no. 4 (April 5, 2021): 347. http://dx.doi.org/10.3390/vaccines9040347.

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The saliva of hematophagous arthropods contains a group of active proteins to counteract host responses against injury and to facilitate the success of a bloodmeal. These salivary proteins have significant impacts on modulating pathogen transmission, immunogenicity expression, the establishment of infection, and even disease severity. Recent studies have shown that several salivary proteins are immunogenic and antibodies against them may block infection, thereby suggesting potential vaccine candidates. Here, we discuss the most relevant salivary proteins currently studied for their therapeutic potential as vaccine candidates or to control the transmission of human vector-borne pathogens and immune responses against different arthropod salivary proteins.
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40

HESTVIK, G., E. WARNS-PETIT, L. A. SMITH, N. J. FOX, H. UHLHORN, M. ARTOIS, D. HANNANT, et al. "The status of tularemia in Europe in a one-health context: a review." Epidemiology and Infection 143, no. 10 (September 30, 2014): 2137–60. http://dx.doi.org/10.1017/s0950268814002398.

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SUMMARYThe bacteriumFrancisella tularensiscauses the vector-borne zoonotic disease tularemia, and may infect a wide range of hosts including invertebrates, mammals and birds. Transmission to humans occurs through contact with infected animals or contaminated environments, or through arthropod vectors. Tularemia has a broad geographical distribution, and there is evidence which suggests local emergence or re-emergence of this disease in Europe. This review was developed to provide an update on the geographical distribution ofF. tularensisin humans, wildlife, domestic animals and vector species, to identify potential public health hazards, and to characterize the epidemiology of tularemia in Europe. Information was collated on cases in humans, domestic animals and wildlife, and on reports of detection of the bacterium in arthropod vectors, from 38 European countries for the period 1992–2012. Multiple international databases on human and animal health were consulted, as well as published reports in the literature. Tularemia is a disease of complex epidemiology that is challenging to understand and therefore to control. Many aspects of this disease remain poorly understood. Better understanding is needed of the epidemiological role of animal hosts, potential vectors, mechanisms of maintenance in the different ecosystems, and routes of transmission of the disease.
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41

Ciota, Alexander T. "Eastern Equine Encephalitis Virus Taxonomy, Genomics, and Evolution." Journal of Medical Entomology 59, no. 1 (November 4, 2021): 14–19. http://dx.doi.org/10.1093/jme/tjab079.

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Abstract Eastern equine encephalitis virus (EEEV; Togaviridae, Alphavirus) is an arthropod-borne virus (arbovirus) primarily maintained in an enzootic cycle between Culiseta melanura (Coquillett) and passerine birds. EEEV, which has the highest reported case- fatality rate among arbovirus in the Americas, is responsible for sporadic outbreaks in the Eastern and Midwest United States. Infection is associated with severe neurologic disease and mortality in horses, humans, and other vertebrate hosts. Here, we review what is known about EEEV taxonomy, functional genomics, and evolution, and identify gaps in knowledge regarding the role of EEEV genetic diversity in transmission and disease.
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42

Estofolete, Cássia F., Bruno H. G. A. Milhim, Nathalia Zini, Samuel N. Scamardi, Joana D’Arc Selvante, Nikos Vasilakis, and Maurício L. Nogueira. "Flavivirus Infection Associated with Cerebrovascular Events." Viruses 12, no. 6 (June 22, 2020): 671. http://dx.doi.org/10.3390/v12060671.

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Arthropod-borne viruses (arboviruses) of the genus Flavivirus are distributed globally and cause significant human disease and mortality annually. Flavivirus infections present a spectrum of clinical manifestations, ranging from asymptomatic to severe manifestations, including hemorrhage, encephalitis and death. Herein, we describe 3 case reports of cerebrovascular involvement in patients infected by dengue and Zika viruses in Sao Jose do Rio Preto, São Paulo State, Brazil, a hyperendemic area for arbovirus circulation, including dengue, yellow fever, chikungunya and Saint Louis encephalitis viruses. Our findings highlight the potential threat that unusual clinical manifestations may pose to arbovirus disease management and recovery.
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Maek-a-nantawat, Wirach, and Udomsak Silachamroon. "Presence of Autoimmune Antibody in Chikungunya Infection." Case Reports in Medicine 2009 (2009): 1–4. http://dx.doi.org/10.1155/2009/840183.

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Chikungunya infection has recently re-emerged as an important arthropod-borne disease in Thailand. Recently, Southern Thailand was identified as a potentially endemic area for the chikungunya virus. Here, we report a case of severe musculoskeletal complication, presenting with muscle weakness and swelling of the limbs. During the investigation to exclude autoimmune muscular inflammation, high titers of antinuclear antibody were detected. This is the report of autoimmunity detection associated with an arbovirus infection. The symptoms can mimic autoimmune polymyositis disease, and the condition requires close monitoring before deciding to embark upon prolonged specific treatment with immunomodulators.
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Linske, Megan A., Kirby C. Stafford, Scott C. Williams, Charles B. Lubelczyk, Margret Welch, and Elizabeth F. Henderson. "Impacts of Deciduous Leaf Litter and Snow Presence on Nymphal Ixodes scapularis (Acari: Ixodidae) Overwintering Survival in Coastal New England, USA." Insects 10, no. 8 (July 30, 2019): 227. http://dx.doi.org/10.3390/insects10080227.

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Blacklegged ticks (Ixodes scapularis Say) are the vector for pathogens that cause more cases of human disease than any other arthropod. Lyme disease is the most common, caused by the bacterial spirochete Borrelia burgdorferi (Johnson, Schmid, Hyde, Steigerwalt, and Brenner) in the northeastern United States. Further knowledge of seasonal effects on survival is important for management and modeling of both blacklegged ticks and tick-borne diseases. The focus of our study was on the impact of environmental factors on overwintering success of nymphal blacklegged ticks. In a three-year field study conducted in Connecticut and Maine, we determined that ground-level conditions play an important role in unfed nymphal overwintering survival. Ticks in plots where leaf litter and snow accumulation were unmanipulated had significantly greater survival compared to those where leaf litter was removed (p = 0.045) and where both leaf litter and snow were removed (p = 0.008). Additionally, we determined that the key overwintering predictors for nymphal blacklegged tick survival were the mean and mean minimum temperatures within a year. The findings of this research can be utilized in both small- and large-scale management of blacklegged ticks to potentially reduce the risk and occurrence of tick-borne diseases.
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45

WANG, H., and P. A. NUTTALL. "Intra-stadial tick-borne Thogoto virus (Orthomyxoviridae) transmission: accelerated arbovirus transmission triggered by host death." Parasitology 122, no. 4 (April 2001): 439–46. http://dx.doi.org/10.1017/s0031182001007478.

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Haematophagous insect vectors of arthropod-borne viruses (arboviruses) feed repeatedly. Consequently, they can transmit arboviruses to more than one host during the same developmental stage (intra-stadial transmission). By contrast, ixodid ticks generally feed only once at each parasitic stage (larva, nymph, and adult) and hence they have only one opportunity for tick-borne virus transmission per stadium (inter-stadial transmission). Under natural conditions, tick-infested hosts may die (from disease or other causes) before the ticks have completed their long period of engorgement. A laboratory model was used to investigate the consequences of premature host death on tick-borne virus transmission. We report intra-stadial transmission of Thogoto virus by the nymphal, male, and female ticks of Rhipicephalus appendiculatus. Tick-borne Thogoto virus infection caused viraemia and death of hamsters before the nymphal and adult ticks feeding on them had completed engorgement. The resulting partially fed ticks were allowed to continue engorgement on new, uninfected hosts (interrupted feeding). During feeding on the subsequent hosts, they transmitted the virus intra-stadially to susceptible hosts (hamsters), and to uninfected co-feeding ticks on non-susceptible hosts (guinea-pigs). Intra-stadial transmission, mediated by interrupted feeding, may help explain outbreaks of rapid and fatal tick-borne viral diseases, and the epidemiology as well as evolution of virulence, in a susceptible host population. Additionally, intra-stadial transmission provides an increased risk of tick-borne pathogen transmission to humans and domestic animals during slaughter and game hunting.
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46

Sroute, Lamyae, Brian D. Byrd, and Scott W. Huffman. "Classification of Mosquitoes with Infrared Spectroscopy and Partial Least Squares-Discriminant Analysis." Applied Spectroscopy 74, no. 8 (August 2020): 900–912. http://dx.doi.org/10.1177/0003702820915729.

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Mosquito-borne diseases are responsible for considerable morbidity and mortality globally. Given the absence of effective vaccines for most arthropod-borne viruses, mosquito control efforts remain the dominant method of disease prevention. Ideal control efforts begin with entomologic surveillance in order to determine the abundance, identity, and infection status of pathogen-vectoring mosquito populations. Traditionally, much of the surveillance work involves morphological species identification by trained entomologists. Limited operational funding and lack of specialized training is a known barrier to surveillance and effective control efforts for many operational mosquito control personnel. Therefore, there is a need for surveillance workflow improvements and rapid mosquito identification methods. Herein, is presented a proof of concept study in which infrared spectroscopy coupled with partial least squares-discriminant analysis was explored as a means of automatically classifying mosquitoes at the species level. The developed method resulted in greater than 94% accuracy for four mosquitoes of public health relevance: Aedes aegypti, Aedes albopictus, Aedes japonicus, and Aedes triseriatus.
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47

Tripathi, Dr Anchal, and Dr Sonali Bhalla. "Wriggly creatures coming out of eye: case report on human ocular thelaziasis." Tropical Journal of Ophthalmology and Otolaryngology 5, no. 8 (December 31, 2020): 250–53. http://dx.doi.org/10.17511/jooo.2020.i08.05.

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Ocular Thelaziasis is an arthropod-borne, zoonotic disease of the eye affecting the conjunctival sac,lacrimal duct, and lacrimal gland caused by a nematode of the genus Thelazia. Thelazia species aretransmitted by different species of Muscidae, which are a family of flies with worldwide distribution.The present study reports a case of human ocular Thelaziasis in a 13-year-old female patient.Species Thelazia callipaeda was confirmed based on microbiological examination. The patient wastreated with anti-helminthic drugs and was relieved of the symptoms without recurrence.
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48

Hoque, Sania, Muhammad Touhidul Islam Khan, and Tausiful Haque. "Chest Pain in a Patient with Dengue Fever Resembling Acute Myocardial Infarction: A Case Report." Journal of Armed Forces Medical College, Bangladesh 17, no. 2 (May 30, 2022): 99–102. http://dx.doi.org/10.3329/jafmc.v17i2.58378.

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Dengue is an arthropod-borne viral disease. Dengue fever may present with dengue hemorrhagic fever/dengue shock syndrome. Now a days some atypical and rare manifestations are noticed involving liver, central nervous system and heart. Here we reported a case of dengue fever in 25 years young man having high level of cardiac biomarkers and ECG changes mimicking myocardial infarction initially but later on further evaluation of the case confirmed as acute myocarditis due to dengue fever. JAFMC Bangladesh. Vol 17, No 2 (December) 2021: 99-102
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Otranto, Domenico. "Arthropod-borne pathogens of dogs and cats: From pathways and times of transmission to disease control." Veterinary Parasitology 251 (February 2018): 68–77. http://dx.doi.org/10.1016/j.vetpar.2017.12.021.

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Kosoltanapiwat, Nathamon, Jarinee Tongshoob, Preeraya Singkhaimuk, Chanyapat Nitatsukprasert, Silas A. Davidson, and Alongkot Ponlawat. "Entomological Surveillance for Zika and Dengue Virus in Aedes Mosquitoes: Implications for Vector Control in Thailand." Pathogens 9, no. 6 (June 4, 2020): 442. http://dx.doi.org/10.3390/pathogens9060442.

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Entomological surveillance for arthropod-borne viruses is vital for monitoring vector-borne diseases and informing vector control programs. In this study, we conducted entomological surveillance in Zika virus endemic areas. In Thailand, it is standard protocol to perform mosquito control within 24 h of a reported dengue case. Aedes females were collected within 72 h of case reports from villages with recent Zika–human cases in Kamphaeng Phet Province, Thailand in 2017 and 2018. Mosquitoes were bisected into head-thorax and abdomen and then screened for Zika (ZIKV) and dengue (DENV) viruses using real-time RT-PCR. ZIKV RNA was detected in three samples from two female Ae. aegypti (1.4%). A partial envelope sequence analysis revealed that the ZIKV sequences were the Asian lineage identical to sequences from ZIKV-infected cases reported in Thailand during 2016 and 2017. Dengue virus-1 (DENV-1) and dengue virus-4 (DENV-4) were found in four Ae. aegypti females (2.8%), and partial capsid sequences were nearly identical with DENV-1 and DENV-4 from Thai human cases reported in 2017. Findings in the current study demonstrate the importance of entomological surveillance programs to public health mosquito-borne disease prevention measures and control.
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