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Journal articles on the topic 'AS-LAMP'

Author: Grafiati

Published: 9 March 2023

Last updated: 31 July 2025

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1

Imam, Husain. "Broad as a lamp, bright as a laser." Nature Photonics 2, no. 1 (2008): 26–28. http://dx.doi.org/10.1038/nphoton.2007.270.

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Hua, Chi T., John J. Hopwood, Sven R. Carlsson, Ray J. Harris, and Peter J. Meikle. "Evaluation of the lysosome-associated membrane protein LAMP-2 as a marker for lysosomal storage disorders." Clinical Chemistry 44, no. 10 (1998): 2094–102. http://dx.doi.org/10.1093/clinchem/44.10.2094.

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Abstract For many lysosomal storage disorders, presymptomatic detection, before the onset of irreversible pathology, will greatly improve the efficacy of current and proposed therapies. In the absence of a family history, presymptomatic detection can be achieved only by a comprehensive newborn screening program. Recently we reported that the lysosome-associated membrane protein LAMP-1 was increased in the plasma from ∼70% of individuals with lysosomal storage disorders. Here we report on the evaluation of a second lysosome-associated membrane protein, LAMP-2, as a marker for this group of diso

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CHANG, Melissa H. Y., Chi T. HUA, Elizabeth L. ISAAC, et al. "Transthyretin interacts with the lysosome-associated membrane protein (LAMP-1) in circulation." Biochemical Journal 382, no. 2 (2004): 481–89. http://dx.doi.org/10.1042/bj20031752.

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LAMP-1 (lysosome-associated membrane protein), a major glycoprotein present in the lysosomal membrane, constitutes up to 50% of total membrane proteins. LAMP-1, expressed at the plasma membrane, is reported to be the major molecule expressing the sialyl-Lewis X antigen. Two forms of LAMP-1 exist; the full-length LAMP-1 [LAMP-1 (+Tail)] has a highly glycosylated lumenal domain, a membrane-spanning domain and a short cytoplasmic tail, and the truncated LAMP-1 [LAMP-1 (−Tail)] contains only the lumenal domain. Soluble LAMP-1 (±Tail) has been reported in circulation. LAMP-1 at the cell surface has

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4

Ko, Jae-jun, and Chung-hyeok Kim. "A Study on the Optical Characteristics of LED Lamp as Alternative Type of FPL Lamp." Journal of the Korean Institute of Illuminating and Electrical Installation Engineers 29, no. 10 (2015): 1–6. http://dx.doi.org/10.5207/jieie.2015.29.10.001.

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5

Ma, C. K., and R. E. Bedford. "Lamp Resistance as a Criterion for Assessing Thermal Equilibrium of the Tungsten Strip-filament Lamp." Metrologia 27, no. 3 (1990): 153–55. http://dx.doi.org/10.1088/0026-1394/27/3/007.

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6

Tanabe, N., K. Go, Y. Sakurada, et al. "A Remote Operating Slit Lamp Microscope System." Methods of Information in Medicine 50, no. 05 (2011): 427–34. http://dx.doi.org/10.3414/me10-01-0064.

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SummaryObjectives: To develop a remote-operating slit lamp microscope system (the remote slit lamp) as the core for highly specialized ophthalmology diagnoses, and to compare the utility of this system with the conventional slit lamp microscope system (the conventional slit lamp) in making a diagnosis.Methods: The remote slit lamp system was developed. Three factors were evaluated in comparison to the conventional slit lamp. The ability to acquire skills was investigated using a task loading system among specialists and residents in ophthalmology. Participants repeated a task up to ten times a

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Nam, Daehan, Seokjoon Kim, Jung Ho Kim, et al. "Low-Temperature Loop-Mediated Isothermal Amplification Operating at Physiological Temperature." Biosensors 13, no. 3 (2023): 367. http://dx.doi.org/10.3390/bios13030367.

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Loop-mediated isothermal amplification (LAMP) is one of the most widely used isothermal amplification technologies in molecular diagnostics. However, LAMP operates at a high temperature of 65 °C; thus, operating LAMP at a lower temperature is desirable to maximize its usefulness for on-site diagnosis. In this study, we propose a new version of LAMP, termed low-temperature LAMP, which operates at the physiological temperature of 37 °C. Low-temperature LAMP differs from conventional LAMP operating at 65 °C in terms of the concentrations of MgSO4 and deoxyribonucleoside triphosphates (dNTPs), as

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Ravindran, Aravind, Julien Levy, Elizabeth Pierson, and Dennis C. Gross. "Development of a Loop-Mediated Isothermal Amplification Procedure as a Sensitive and Rapid Method for Detection of ‘Candidatus Liberibacter solanacearum’ in Potatoes and Psyllids." Phytopathology® 102, no. 9 (2012): 899–907. http://dx.doi.org/10.1094/phyto-03-12-0055-r.

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This study reports the development of a loop-mediated isothermal amplification procedure (LAMP) for polymerase chain reaction (PCR)-based detection of ‘Candidatus Liberibacter solanacearum’, the bacterial causal agent of potato zebra chip (ZC) disease. The 16S rDNA gene of ‘Ca. Liberibacter solanacearum’ was used to design a set of six primers for LAMP PCR detection of the bacterial pathogen in potato plants and the psyllid vector. The advantage of the LAMP method is that it does not require a thermocycler for amplification or agarose gel electrophoresis for resolution. Positive LAMP results c

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9

Silverstein, RL, and M. Febbraio. "Identification of lysosome-associated membrane protein-2 as an activation-dependent platelet surface glycoprotein." Blood 80, no. 6 (1992): 1470–75. http://dx.doi.org/10.1182/blood.v80.6.1470.1470.

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Abstract Platelets undergo biochemical and morphologic changes when stimulated that greatly alter their function and contribute to their role in thrombosis and hemostasis. We recently identified and cloned the cDNA for a platelet surface glycoprotein expressed on activated, not resting cells. We found that this protein, lysome-associated membrane protein-1 (LAMP-1), is an integral membrane protein of the lysosome that translocated to the surface membrane when platelets were stimulated by a strong agonist. We now show with immunofluorescence flow cytometry that LAMP-2, a lysosomal membrane prot

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10

Silverstein, RL, and M. Febbraio. "Identification of lysosome-associated membrane protein-2 as an activation-dependent platelet surface glycoprotein." Blood 80, no. 6 (1992): 1470–75. http://dx.doi.org/10.1182/blood.v80.6.1470.bloodjournal8061470.

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Platelets undergo biochemical and morphologic changes when stimulated that greatly alter their function and contribute to their role in thrombosis and hemostasis. We recently identified and cloned the cDNA for a platelet surface glycoprotein expressed on activated, not resting cells. We found that this protein, lysome-associated membrane protein-1 (LAMP-1), is an integral membrane protein of the lysosome that translocated to the surface membrane when platelets were stimulated by a strong agonist. We now show with immunofluorescence flow cytometry that LAMP-2, a lysosomal membrane protein that

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11

Lee, Pui-Yuei, Yien-Ping Wong, Shuhaidah Othman, and Hui-Yee Chee. "Room-temperature stable loop-mediated isothermal amplification (LAMP) reagents to detect leptospiral DNA." Asian Biomedicine 15, no. 4 (2021): 183–89. http://dx.doi.org/10.2478/abm-2021-0023.

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Abstract Background Loop-mediated isothermal amplification (LAMP) is one of the most promising tools for rapidly detecting Leptospira spp. However, LAMP is hampered by cold storage to maintain the enzymatic activity of Bst DNA polymerase. Objective To overcome the drawback of cold storage requirement for LAMP reagents we modified the reagents by adding sucrose as stabilizer. We then sought to determine the stability at room temperature of the premixed LAMP reagents containing sucrose. Method Premixed LAMP reagents with sucrose and without sucrose were prepared. The prepared mixtures were store

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Lamalee, Aekarin, Soithong Saiyudthong, Chartchai Changsen, et al. "End-point rapid detection of total and pathogenic Vibrio parahaemolyticus (tdh⁺ and/or trh1⁺ and/or trh2⁺) in raw seafood using a colorimetric loop-mediated isothermal amplification-xylenol orange technique." PeerJ 12 (January 3, 2024): e16422. http://dx.doi.org/10.7717/peerj.16422.

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Background Vibrio parahaemolyticus is the leading cause of bacterial seafood-borne gastroenteritis in humans worldwide. To ensure seafood safety and to minimize the occurrence of seafood-borne diseases, early detection of total V. parahaemolyticus (pathogenic and non-pathogenic strains) and pathogenic V. parahaemolyticus (tdh+ and/or trh1+ and/or trh2+) is required. This study further improved a loop-mediated isothermal amplification (LAMP) assay using xylenol orange (XO), a pH sensitive dye, to transform conventional LAMP into a one-step colorimetric assay giving visible results to the naked

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Mohd Khairudin, Rahman, M. I. Mohd Hafzi, and Hamzan Azhar. "Amber Position Lamp as Daytime Running Light for Motorcycle." Advanced Engineering Forum 10 (December 2013): 357–60. http://dx.doi.org/10.4028/www.scientific.net/aef.10.357.

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The ability for motorcycle to be detected is an important aspect in preventing crash involving motorcycle which is the most dominant vehicle in emerging countries. Widely referred as conspicuity, the crash factor is appropriately addressed by the introduction of mandatory daytime running light (DRL) law and is usually a success story in many parts of the world. In 2011, there was a motion introduced in the 64thsession of the United Nations Working Party on Lighting and Light-Signalling (GRE) for amber position lamp (APL) to be made mandatory on motorcycle as additional measure to improve motor

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Netongo, Palmer Masumbe, Severin Donald Kamdem, Ardin Noutong Ouayoue, et al. "Noninvasive Approach of Plasmodium falciparum Molecular Detection for Malaria Surveillance in Malaria Endemic Areas in Cameroon." BioMed Research International 2022 (November 9, 2022): 1–8. http://dx.doi.org/10.1155/2022/3600354.

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Background. Accurate, cost-effective, and noninvasive alternative molecular methods are needed for detecting low malaria parasitemia. The currently-used nested polymerase chain reaction (nPCR) requires blood as well as skilled personnel in order to minimise the risk of bloodborne disease transmission. Therefore, this study is aimed at assessing the accuracy of a noninvasive and more affordable malaria diagnosis with saliva using the loop-mediated isothermal amplification (LAMP) technique. Methods. A cross-sectional study was conducted in the Centre and Southwest regions of Cameroon. Matched bl

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15

Saito, Yuichi, Atsuka Matsui, Satoru Michiyuki, et al. "Loop-Mediated Isothermal Amplification as Point-of-Care Testing for EGFR-Mutated Lung Adenocarcinoma." Micromachines 13, no. 6 (2022): 897. http://dx.doi.org/10.3390/mi13060897.

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Liquid biopsy has been adapted as a diagnostic test for EGFR mutations in patients with advanced or metastatic non-small cell lung cancer (NSCLC). Loop-mediated isothermal amplification (LAMP) has been widely used for the rapid detection of pathogens through DNA amplification. This study investigated the efficacy of an EGFR-LAMP assay using plasma samples of patients with resected NSCLC tumors. The EGFR status was investigated using both LAMP and next-generation sequencing (NGS) assays in cases that met the following criteria: (1) pulmonary adenocarcinoma with EGFR mutation detected by the The

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Chang, TaC, and Matteo Ziff. "Using smartphone flashlight as slit lamp light source." Indian Journal of Ophthalmology 68, no. 8 (2020): 1658. http://dx.doi.org/10.4103/ijo.ijo_2335_19.

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17

Sung, Wen-Tsai, Ihzany Vilia Devi, and Sung-Jung Hsiao. "Smart Lamp Using Google Firebase as Realtime Database." Intelligent Automation & Soft Computing 33, no. 2 (2022): 967–82. http://dx.doi.org/10.32604/iasc.2022.024664.

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18

Schulman, M. B., and D. R. Woodward. "Plasma‐enhanced photoemission as a discharge lamp diagnostic." Applied Physics Letters 55, no. 16 (1989): 1618–20. http://dx.doi.org/10.1063/1.102216.

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Arbaciauskaite, Skaiste, Pouya Babakhani, Natalia Sandetskaya, et al. "Self-Sampled Gargle Water Direct RT-LAMP as a Screening Method for the Detection of SARS-CoV-2 Infections." Diagnostics 12, no. 4 (2022): 775. http://dx.doi.org/10.3390/diagnostics12040775.

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We assessed the viability of self-sampled gargle water direct RT-LAMP (LAMP) for detecting SARS-CoV-2 infections by estimating its sensitivity with respect to the gold standard indirect RT-PCR of paired oro-nasopharyngeal swab samples. We also assessed the impact of symptom onset to test time (STT)—i.e., symptom days at sampling, on LAMP. In addition, we appraised the viability of gargle water self-sampling versus oro-nasopharyngeal swab sampling, by comparing paired indirect RT-PCR results. 202 oro-nasopharyngeal swab and paired self-sampled gargle water samples were collected from hospital p

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20

Rhee, S. W., H. S. Park, and H. H. Choi. "Comparison Of Mercury Distribution Between The Types Of Spent Fluorescent Lamp." Archives of Metallurgy and Materials 60, no. 2 (2015): 1297–99. http://dx.doi.org/10.1515/amm-2015-0117.

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Abstract Spent fluorescent lamps(SFLs) such as linear type lamp, compact type lamp and U-type lamp are used to estimate mercury distribution in the components of lamps. Determination of mercury concentration in the components of spent fluorescent lamp is performed by the DMA method. Mercury concentration in the components of spent fluorescent lamp can be varied with the manufactures of lamp. Mercury portion in phosphor powder and glass from any types of spent fluorescent lamp is estimated to be higher than 99% by the analysis of mercury distribution. Through mercury distribution in the compone

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Pérez, Liliana, Sarah Deffit, and Janice Blum. "LAMP-2C regulation of MHC class II antigen presentation (APP3P.105)." Journal of Immunology 192, no. 1_Supplement (2014): 111.6. http://dx.doi.org/10.4049/jimmunol.192.supp.111.6.

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Abstract In autophagy cells divert cytoplasmic components and organelles to lysosomes for degradation impacting innate and adaptive immunity. A selective form of autophagy, known as chaperone-mediated autophagy (CMA), is implicated in adaptive immunity. CMA relies on the lysosome-associated membrane protein (LAMP)-2A to translocate proteins from the cytoplasm into lysosomes. Alternative splicing of the LAMP-2 gene generates 3 highly conserved isoforms LAMP-2A, LAMP-2B and LAMP-2C. The function and regulation of these genes is not well understood. Here we identified a novel role for LAMP-2C in

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Li, Tongzhe, and Hongkui Zhang. "Design of explosion-proof induction lamp temperature test system." Highlights in Science, Engineering and Technology 27 (December 27, 2022): 691–95. http://dx.doi.org/10.54097/hset.v27i.3833.

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Temperature test is an item to be tested in the type test of explosion-proof induction lamp. Temperature test is an item to be tested in the type test of explosion-proof induction lamp. A temperature test system based on single chip microcomputer integrated control and automatic trigger is proposed to improve the efficiency and stability of temperature test of explosion-proof induction lamp. Based on the analysis of the working principle of the explosion-proof induction lamp, the explosion-proof induction lamp temperature test system is designed, and the full-power trigger module of the explos

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23

Ku, Jamin, Khushbu Chauhan, Sang-Hyun Hwang, Yong-Joo Jeong, and Dong-Eun Kim. "Enhanced Specificity in Loop-Mediated Isothermal Amplification with Poly(ethylene glycol)-Engrafted Graphene Oxide for Detection of Viral Genes." Biosensors 12, no. 8 (2022): 661. http://dx.doi.org/10.3390/bios12080661.

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Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method that allows the simple, quick, and low-cost detection of various viral genes. LAMP assays are susceptible to generating non-specific amplicons, as high concentrations of DNA primers can give rise to primer dimerization and mismatched hybridizations, resulting in false-positive signals. Herein, we reported that poly(ethylene glycol)-engrafted nanosized graphene oxide (PEG-nGO) can significantly enhance the specificity of LAMP, owing to its ability to adsorb single-stranded DNA (ssDNA). By adsorbing surplus ssDN

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Chi, Congwu, Andrea Leonard, Walter E. Knight, et al. "LAMP-2B regulates human cardiomyocyte function by mediating autophagosome–lysosome fusion." Proceedings of the National Academy of Sciences 116, no. 2 (2018): 556–65. http://dx.doi.org/10.1073/pnas.1808618116.

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Mutations in lysosomal-associated membrane protein 2 (LAMP-2) gene are associated with Danon disease, which often leads to cardiomyopathy/heart failure through poorly defined mechanisms. Here, we identify the LAMP-2 isoform B (LAMP-2B) as required for autophagosome–lysosome fusion in human cardiomyocytes (CMs). Remarkably, LAMP-2B functions independently of syntaxin 17 (STX17), a protein that is essential for autophagosome–lysosome fusion in non-CMs. Instead, LAMP-2B interacts with autophagy related 14 (ATG14) and vesicle-associated membrane protein 8 (VAMP8) through its C-terminal coiled coil

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YOKOYAMA, EIJI, MASAKO UCHIMURA, and KENITIRO ITO. "Detection of Enteroaggregative Escherichia coli by Loop-Mediated Isothermal Amplification." Journal of Food Protection 73, no. 6 (2010): 1064–72. http://dx.doi.org/10.4315/0362-028x-73.6.1064.

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A novel gene amplification method, loop-mediated isothermal amplification (LAMP), has been recently developed as a rapid, specific diagnostic method for various infectious diseases. We have investigated whether LAMP can be used to detect small numbers of enteroaggregative Escherichia coli (EAEC) cells contaminated in food samples. Primers for LAMP reaction were designed with EAEC aggR gene sequences (available in GenBank). LAMP specificity with these primers was the same as that of PCR in a study of 37 EAEC and 42 non-EAEC bacterial strains. The sensitivity of the LAMP method was better than t

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Taufiq, Mulyono Sumitro Baskoro, Wazir Mawardi, and Mochammad Riyanto. "ENGINEERING PORTABLE UNDERWATER LAMP AS AN AUXILIARY GEAR FOR PURSE SEINE." Marine Fisheries : Journal of Marine Fisheries Technology and Management 14, no. 1 (2023): 25–37. http://dx.doi.org/10.29244/jmf.v14i1.43216.

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Lights are common supporting tool in purse seine fishing in Indonesia. Currently, lamps on purse seines use mercury and metal halide (MH) which require very high electrical power. Therefore, there is a need for an effective underwater light technology alternative with low electric power. The aim of this research is to design a portable underwater lamp (PUL) which can be controlled remotely to support fishing operations. The design engineering process in creating the PUL includes light and buoy construction, temperature testing, light distribution, waterproofing, and motion testing. The results

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Murwantoko, Murwantoko. "Metode Loop-Mediated Isothermal Amplification (LAMP) dan Aplikasinya untuk Deteksi Penyakit Ikan." Jurnal Perikanan Universitas Gadjah Mada 8, no. 1 (2006): 1. http://dx.doi.org/10.22146/jfs.156.

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Polymerase chain reaction (PCR) is a method that amplifies DNA which have been widely used in molecular biology technique. Based on the PCR, many methods have been developed on isothermal condition and the useful one is loop-mediated isothermal amplification of DNA (LAMP). LAMP reaction employs a Bst DNA polymerase and a set of four specific primers that recognizes a total of six distinct sequences of the target DNA and produces amount of different size of DNA. Many advantages have been achieved in LAMP such as the simple equipment for reaction and observation, short time, highly specific and

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Eskelinen, Eeva-Liisa, Christine Katrin Schmidt, Silja Neu, et al. "Disturbed Cholesterol Traffic but Normal Proteolytic Function in LAMP-1/LAMP-2 Double-deficient Fibroblasts." Molecular Biology of the Cell 15, no. 7 (2004): 3132–45. http://dx.doi.org/10.1091/mbc.e04-02-0103.

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Mice double deficient in LAMP-1 and -2 were generated. The embryos died between embryonic days 14.5 and 16.5. An accumulation of autophagic vacuoles was detected in many tissues including endothelial cells and Schwann cells. Fibroblast cell lines derived from the double-deficient embryos accumulated autophagic vacuoles and the autophagy protein LC3II after amino acid starvation. Lysosomal vesicles were larger and more peripherally distributed and showed a lower specific density in Percoll gradients in double deficient when compared with control cells. Lysosomal enzyme activities, cathepsin D p

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Wang, Yin, and Yun Zhe Zhang. "Optimization of Energy-Saving Lighting Line Adjustable Research." Applied Mechanics and Materials 668-669 (October 2014): 884–87. http://dx.doi.org/10.4028/www.scientific.net/amm.668-669.884.

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Energy-saving lamp will still be a favorite product on the lighting market in the long run. One of the drawbacks of common energy saving lamp is that it cannot be dimmed. Energy is wasted when bright light is not necessary. To promote the idea of energy saving and environmental protection, a plan for the applicability of dimming of energy saving lamp is in urgent need. In this study, we put forward two plans for the dimming of energy saving lamp. Our effort is to make the energy saving lamp adapted to the variety of power frequency and to maximize its smooth dimming as close to that of an inca

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Peng, Huan, Deliang Peng, Xianqi Hu, et al. "Loop-mediated isothermal amplification for rapid and precise detection of the burrowing nematode, Radopholus similis, directly from diseased plant tissues." Nematology 14, no. 8 (2012): 977–86. http://dx.doi.org/10.1163/156854112x638415.

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A novel, simple, rapid and highly sensitive assay and diagnostic tool for the burrowing nematode, Radopholus similis, was developed using a loop-mediated isothermal amplification (LAMP). The LAMP assay was targeted on the specific fragments of rRNA gene D2-D3 regions of R. similis. The detection limitation of the LAMP assay was as low as ten copies of plasmid DNA containing the target DNA, 10 fg of genomic DNA and 5 × 10−5 nematodes. The detection sensitivity of the LAMP method for R. similis DNA was 10-100 times higher than normal PCR-based detection methods. The LAMP amplifications could be

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Yanagihara, Masahiro, Takayuki Tsuji, Mohd Zamri Yusop, et al. "Vacuum Ultraviolet Field Emission Lamp Consisting of Neodymium Ion Doped Lutetium Fluoride Thin Film as Phosphor." Scientific World Journal 2014 (2014): 1–5. http://dx.doi.org/10.1155/2014/309091.

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A vacuum ultraviolet (VUV) field emission lamp was developed by using a neodymium ion doped lutetium fluoride (Nd3+ : LuF3) thin film as solid-state phosphor and carbon nanofiber field electron emitters. The thin film was synthesized by pulsed laser deposition and incorporated into the lamp. The cathodoluminescence spectra of the lamp showed multiple emission peaks at 180, 225, and 255 nm. These emission spectra were in good agreement with the spectra reported for the Nd3+ : LuF3crystal. Moreover, application of an acceleration voltage effectively increased the emission intensity. These result

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Sohrabi, Amir, Joar Franzen, Nikolaos Tertipis, et al. "Efficacy of Loop-Mediated Isothermal Amplification for H. pylori Detection as Point-of-Care Testing by Noninvasive Sampling." Diagnostics 11, no. 9 (2021): 1538. http://dx.doi.org/10.3390/diagnostics11091538.

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For targeted eradication of Helicobacter pylori (H. pylori) to reduce gastric cancer burden, a convenient approach is definitely needed. The purpose of this study was to evaluate the LAMP assay for H. pylori detection using samples collected by noninvasive and self-sampling methods. The available LAMP assay for H. pylori detection was appraised and verified using reference and clinically isolated H. pylori strains. In addition, a clinical study was conducted to assess the LAMP assay on 51 patients, from whom saliva, oral brushing samples, feces, corpus, and antrum specimens were available. Cla

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Tie, Zhang, Wang Chunguang, Wei Xiaoyuan, Zhao Xinghua, and Zhong Xiuhui. "Loop-Mediated Isothermal Amplification for Detection ofStaphylococcus aureusin Dairy Cow Suffering from Mastitis." Journal of Biomedicine and Biotechnology 2012 (2012): 1–5. http://dx.doi.org/10.1155/2012/435982.

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To develop a rapid detection method ofStaphylococcus aureususing loop-mediated isothermal amplification (LAMP), four specific primers were designed according to six distinct sequences of thenucgene. In addition, the specificity and sensitivity of LAMP were verified and compared with those of PCR. Results showed that the LAMP reaction was completed within 45 min at 62.5°C, and ladder bands were appeared in LAMP products analyzed by gel electrophoresis. After adding 1x SYBR Green l, the positive reaction tube showed green color and the negative reaction tube remained orange, indicating that the

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Zheng, Youbin, Ping Zhang, and Mike Dixon. "Evaluation of Four Lamp Types for the Production of Tomato Plants in Controlled Environments." HortTechnology 15, no. 3 (2005): 646–52. http://dx.doi.org/10.21273/horttech.15.3.0646.

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To evaluate the performance of four newly developed high-intensity-discharge lamp types on plant growth and production, tomato (Lycopersicon esculentum cv. Tradiro F1) plants were grown indoors under 100% artificial lighting for 17 weeks. The four lamp types were: high-pressure sodium high output [HPS(HO)], high-pressure sodium standard [HPS(STD)], metal halide warm deluxe [MH(WDX)] and metal halide cool deluxe [MH(CDX)]. All the lamps tested were 1000 W. HPS(HO) had the highest electrical energy use efficiency (EUE) (0.98 μmol·m–2·s–1·W–1 at 40 cm directly under the lamp); HPS(STD), MH(WDX) a

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Israels, S. J., E. M. McMillan, C. Robertson, S. Singhroy, and A. McNicol. "The Lysosomal Granule Membrane Protein, Lamp-2, Is also Present in Platelet Dense Granule Membranes." Thrombosis and Haemostasis 75, no. 04 (1996): 623–29. http://dx.doi.org/10.1055/s-0038-1650333.

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SummaryLysosomal Associated Membrane Protein-2 (LAMP-2) is an inherent component of lysosomal granule membranes in diverse cell types, including platelets. We examined platelets for evidence of LAMP-2 in dense granule membranes as CD63 has previously been shown to be present in both lysosomal and dense granule membranes. Immunological techniques were used to examine the localization of LAMP-2 in control platelets and those from an individual with Hermansky-Pudlak syndrome (HPS), a condition characterised by platelet dense granule deficiency. Immunoblotting studies demonstrated that LAMP-2 was

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Mannier, Cassidy, and Jeong-Yeol Yoon. "Progression of LAMP as a Result of the COVID-19 Pandemic: Is PCR Finally Rivaled?" Biosensors 12, no. 7 (2022): 492. http://dx.doi.org/10.3390/bios12070492.

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Reflecting on the past three years and the coronavirus disease 19 (COVID-19) pandemic, varying global tactics offer insights into the most effective public-health responses. In the US, specifically, rapid and widespread testing was quickly prioritized to lower restrictions sooner. Essentially, only two types of COVID-19 diagnostic tests were publicly employed during the peak pandemic: the rapid antigen test and reverse transcription polymerase chain reaction (RT-PCR). However, neither test ideally suited the situation, as rapid antigen tests are far too inaccurate, and RT-PCR tests require ski

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Morris, Ulrika, and Berit Aydin-Schmidt. "Performance and Application of Commercially Available Loop-Mediated Isothermal Amplification (LAMP) Kits in Malaria Endemic and Non-Endemic Settings." Diagnostics 11, no. 2 (2021): 336. http://dx.doi.org/10.3390/diagnostics11020336.

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Loop-mediated isothermal amplification (LAMP) is a sensitive molecular tool suitable for use as a near point-of-care test for the diagnosis of malaria. Recent meta-analyses have detailed high sensitivity and specificity of malaria LAMP when compared to microscopy, rapid diagnostic tests, and polymerase chain reaction in both endemic and non-endemic settings. Despite this, the use of malaria LAMP has primarily been limited to research settings to date. In this review, we aim to assess to what extent commercially available malaria LAMP kits have been applied in different settings, and to identif

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Zhang, Yinhua, and Nathan A. Tanner. "Improving RT-LAMP detection of SARS-CoV-2 RNA through primer set selection and combination." PLOS ONE 17, no. 4 (2022): e0254324. http://dx.doi.org/10.1371/journal.pone.0254324.

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Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has emerged as a viable molecular diagnostic method to expand the breadth and reach of nucleic acid testing, particularly for SARS-CoV-2 detection and surveillance. While rapidly growing in prominence, RT-LAMP remains a relatively new method compared to the standard RT-qPCR, and contribution to our body of knowledge on designing LAMP primer sets and assays can have significant impact on its utility and adoption. Here we select and evaluate 18 LAMP primer sets for SARS-CoV-2 previously identified as sensitive ones under vari

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Li, Zhengkang, Yuwei Di, Xiaoyan Song, et al. "Rapid Detection of Norovirus GII by Fluorescent Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) and Nanomagnetic Bead Separation." Journal of Biomedical Nanotechnology 19, no. 8 (2023): 1413–21. http://dx.doi.org/10.1166/jbn.2023.3552.

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Noroviruses (NoVs) is the main cause of gastroenteritis in humans worldwide, mainly affecting school-age children and adults. NoVs are transmitted through feces and vomitus, including human contact, food, and water. Presently, NoVs are detected using molecular biological methods. Loop-mediated isothermal amplification (LAMP), specifically, requires little detection equipment, a short detection time, and low technical skills. Here, we established our own NoV reverse transcription (RT) polymerase chain reaction (PCR) quantitative detection system and a NoV GII RT-LAMP detection system. We collec

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Hooker, Edmond A., William J. Faulkner, Lisa D. Kelly, and Robert C. Whitford. "Prospective study of the sensitivity of the Wood’s lamp for common eye abnormalities." Emergency Medicine Journal 36, no. 3 (2019): 159–62. http://dx.doi.org/10.1136/emermed-2018-208235.

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ObjectiveThe Wood’s lamp, a handheld instrument that uses long-wave ultraviolet (UV) light with magnification of 2–3 times, is commonly used by non-ophthalmologists for examining patients with eye complaints. The goal of current research was to determine the sensitivity and specificity of the Wood’s lamp for common eye abnormalities.Study designWe examined a convenience sample of patients, 18 years of age and older, who presented for eye complaints to an urgent clinic of a large ophthalmology practice. This prospective observational trial was performed from December 2016 until July 2017. An op

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Yoshikawa, Rokusuke, Haruka Abe, Yui Igasaki, Saeki Negishi, Hiroaki Goto, and Jiro Yasuda. "Development and evaluation of a rapid and simple diagnostic assay for COVID-19 based on loop-mediated isothermal amplification." PLOS Neglected Tropical Diseases 14, no. 11 (2020): e0008855. http://dx.doi.org/10.1371/journal.pntd.0008855.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly pathogenic novel coronavirus that has caused a worldwide outbreak. Here we describe a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that uses a portable device for efficient detection of SARS-CoV-2. This RT-LAMP assay specifically detected SARS-CoV-2 without cross-reacting with the most closely related human coronavirus, SARS-CoV. Clinical evaluation of nasal swab samples from suspected SARS-CoV-2 pneumonia (COVID-19) patients showed that the assay could detect over 23.7 copies within 15 min

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Mayor-Smith, Ian, and Michael R. Templeton. "Methodological considerations when conducting bench scale polychromatic ultraviolet irradiation of water." Water Supply 14, no. 2 (2013): 291–98. http://dx.doi.org/10.2166/ws.2013.202.

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The use of a bench scale apparatus (often referred to as a ‘collimated beam’) to apply fluences to water samples is common practice in disinfection research and in validating the performance of full-scale UV disinfection reactors. This study investigated the sources of potential experimental variations in the calculation of fluence when conducting polychromatic collimated beam exposures. Spectral variations associated with lamp operating conditions (e.g. cooling of the lamp), the angle of the spectroradiometer relative to the lamp when measuring the UV fluence rate, and the shape of the arc wi

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Zhukareva, V., and P. Levitt. "The limbic system-associated membrane protein (LAMP) selectively mediates interactions with specific central neuron populations." Development 121, no. 4 (1995): 1161–72. http://dx.doi.org/10.1242/dev.121.4.1161.

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The limbic system-associated membrane protein (LAMP) is a 64–68 × 10(3) M(r) glycoprotein that is expressed by subsets of neurons that are functionally interconnected. LAMP exhibits characteristics that are indicative of a developmentally significant protein, such as an early and restricted pattern of expression and the ability to mediate specific fiber-target interactions. A potential, selective adhesive mechanism by which LAMP may regulate the formation of specific circuits is investigated in the present experiments. LAMP is readily released from intact membranes by phosphatidyl inositol-spe

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Feranisa, Anggun. "KOMPARASI ANTARA POLYMERASE CHAIN REACTION (PCR) DAN LOOPMEDIATED ISOTHERMAL AMPLIFICATION (LAMP) DALAM DIAGNOSIS MOLEKULER." ODONTO : Dental Journal 3, no. 2 (2016): 145. http://dx.doi.org/10.30659/odj.3.2.145-151.

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Background: Molecular diagnostic is an emerging diagnostic method inpersonalized medicine/dentistry era. Usually, it uses nucleic acid amplificationmethod to detect various diseases. PCR is conventional nucleic acid amplification method. However, due to an urgency in infectious diseases’ diagnotic method, scientists developed LAMP as new nucleic acid amplification method.Discussion: There are various experiments used to develop LAMP as infectious diseases diagnostic method compared to PCR. The results are LAMP more sensitive, specific, rapid, and inexpensive than PCR.Conclusion: Both PCR and L

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Guo, Jia Xu, and Ling Long. "The Intelligent Desk Lamp Designed for Special Populations." Applied Mechanics and Materials 571-572 (June 2014): 980–84. http://dx.doi.org/10.4028/www.scientific.net/amm.571-572.980.

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For senior citizens and people with disabilities and other special groups, it is inconvenient for them to use the ordinary desk lamp. This essay presents a design scheme of intelligent desk lamp based on infrared detector. Pyroelectric infrared sensor can detect infrared radiation of human, which can be used as the automatic switch to solve the limitation of the manual switch for these special groups. Intelligent desk lamp can meet these crowds on the specialized requirements of everyday lighting lamp according the characteristic. Compared with the traditional household table lamp, this design

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Boyd, Douglas, and Thomas Guinn. "Efficacy of the Localized Aviation MOS Program in Ceiling Flight Category Forecasts." Atmosphere 10, no. 3 (2019): 127. http://dx.doi.org/10.3390/atmos10030127.

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(1) Background: Flying in instrument meteorological conditions (IMC) carries an elevated risk of fatal outcome for general aviation (GA) pilots. For the typical GA flight, aerodrome-specific forecasts (Terminal Aerodrome Forecast (TAF), Localized Aviation Model Output Statistics Program (LAMP)) assist the airman in pre-determining whether a flight can be safely undertaken. While LAMP forecasts are more prevalent at GA-frequented aerodromes, the Federal Aviation Administration (FAA) recommends that this tool be used as supplementary to the TAF only. Herein, the predictive accuracy of LAMP for c

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Mamba, Timothy S., Cecilia K. Mbae, Johnson Kinyua, Erastus Mulinge, Gitonga Nkanata Mburugu, and Zablon K. Njiru. "Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection ofCryptosporidiumSpecies in Kenyan Children Presenting with Diarrhea." Journal of Tropical Medicine 2018 (2018): 1–9. http://dx.doi.org/10.1155/2018/7659730.

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Background. Cryptosporidiumis a protozoan parasite and a major cause of diarrhea in children and immunocompromised patients. Current diagnostic methods for cryptosporidiosis such as microscopy have low sensitivity while techniques such as PCR indicate higher sensitivity levels but are seldom used in developing countries due to their associated cost. A loop-mediated isothermal amplification (LAMP) technique, a method with shorter time to result and with equal or higher sensitivity compared to PCR, has been developed and applied in the detection ofCryptosporidiumspecies. The test has a detection

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Assiddiq S, Hasbi, Jeffry Anggara, and Hairul Anwar. "The DESIGN OF A HYDROELECTRIC POWER PLANT IS KIND OF A PELTON TURBINE ON A LABORATORY SCALE AS A LEARNING MEDIA." Teknika STTKD: Jurnal Teknik, Elektronik, Engine 9, no. 1 (2023): 107–16. http://dx.doi.org/10.56521/teknika.v9i1.865.

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The power plant type turbine pelton of scale laboratory was made to be used as a practical medium of students and lecturers of mechanical engineering in the environment Politeknik of Kotabaru. The purpose of this research was to obtain an ideal design for hydropower systems as learning media; Knowing the effect of the lamp load on the generator rotation, knowing the effect of the type of 12 VDC and 24 VDC lamp loads with varying loads on the generator power output. The method used in this study is a literature study and experimental methods. The research results obtained are the design has a t

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Lai, Meng-Yee, Mohd Hafizi Abdul Hamid, Jenarun Jelip, Rose Nani Mudin, and Yee-Ling Lau. "Evaluation of A Simple DNA Extraction Method and Its Combination with Loop-Mediated Isothermal Amplification Assays for Rapid Plasmodium knowlesi Diagnosis." Tropical Medicine and Infectious Disease 8, no. 8 (2023): 389. http://dx.doi.org/10.3390/tropicalmed8080389.

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The initial and vital stage in the diagnosis of malaria involves extracting DNA. The efficiency of malaria testing is restricted by the multiple steps involved in commercial DNA extraction kits. We attempted to improve an existing loop-mediated isothermal amplification (LAMP) for the detection of Plasmodium knowlesi by using a simple DNA extraction approach, making it a feasible option for mass screening. We utilized a simple nucleic acid extraction method directly from whole blood for the detection of P. knowlesi, taking only 5 min to complete. The extracted DNA was evaluated by two fluoresce

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PARTHIBAN, M., K. S. SHALINI, KURUNCHI C. DIVYA, K. KUMANAN, and K. S. AARTHI. "Rapid detection of fowl adenovirus field samples using loop-mediated isothermal amplification assay." Indian Journal of Animal Sciences 84, no. 1 (2014): 22–25. http://dx.doi.org/10.56093/ijans.v84i1.37295.

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Loop-mediated isothermal amplification was employed for the detection of fowl adenoviruses as a simple, rapid and field based diagnostic assay. Out of 134 field samples screened, 28 samples were positive by both PCR and LAMP assay. The LAMP assay primers were found to be highly specific and it detected only fowl adenovirus samples and it did not react with other avian viruses like Marek’s disease virus and avian leucosis virus. Conventional PCR detected 100ug level of DNA whereas LAMP assay detected up to 10ng level of DNA. It clearly indicated that LAMP assay was 100-times more sensitive than

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