Academic literature on the topic 'Aseptic Seedling'
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Journal articles on the topic "Aseptic Seedling"
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Zhang, Haibo, Di He, Xiaoling Li, Bicheng Dun, Di Wu, and Guiyun Huang. "The Establishment of Rapid Propagation System of ‘RED SUN’ Phalaenopsis aphrodite." Sustainability 14, no. 22 (2022): 15305. http://dx.doi.org/10.3390/su142215305.
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Phalaenopsis aphrodite Rchb. F. is a plant of great ornamental and economic value, and its growth has strict requirements in terms of environment. In order to establish the rapid propagation of Phalaenopsis aphrodite Rchb. F., this study used the popular large-flowered variety ‘RED SUN’, which is found on the market, as the material, and studied the effects of pedicel taking time, site, plant growth regulators, and organic substances on the establishment of a regeneration system. The results indicated that the best combination of initiation culture was collecting peduncles in January + upper position of peduncles + 0.1% HgCl2 sterilization for 8 min. The best recipe for inducing adventitious buds was MS + TDZ 0.4 mg/L + NAA 0.5 mg/L. The optimal combination for adventitious bud propagation was MS + TDZ 0.2 mg/L + NAA 0.4 mg/L. The best recipe for strong seedling from aseptic seedlings was MS + NAA 0.4 mg/L + banana powder 20 g/L + tryptone 2 g. The best recipe for rooting for aseptic seedlings was 1/2 MS + IBA 0.5 mg/L + charcoal 2 g/L. The best combination of acclimation and transplanting for Phalaenopsis was using sphagnum in the training seedling room, which produced a seedling survival rate of 97%. Our findings, therefore, demonstrate the methods of rapid breeding of the Phalaenopsis orchid, which provide a scientific basis for the conservation of this species.
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Twardovska, M. O., I. O. Andreev, and V. A. Kunakh. "Introduction into in vitro culture and cytogenetic analysis of Iris attica Boiss. & Heldr. and Iris pseudopumila Tineo plants." Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 16, no. 2 (2019): 203–11. http://dx.doi.org/10.7124/visnyk.utgis.16.2.1058.
Full textAbstract:
Aim. The work was aimed at the development of conditions for introduction into in vitro culture of two species of irises, Iris attica and I. pseudopumila to obtain aseptic seedlings with subsequent reintroduction into natural environment, as well as at cytogenetic analysis of the obtained plants. Methods. In vitro seed germination and seedling cultivation. Cytogenetic analysis of cells of root meristem, determination of chromosome number and morphology in mitotic metaphase plates, anaphase analysis. Results. The plants of I. attica and I. pseudopumila were introduced in vitro. Aseptic seedlings were obtained, which were actively growing on MS/2 medium without phytohormones. The experiments on the adaptation of the plants to greenhouse conditions revealed the high survival rate for both species. The chromosome number 2n = 16 was established for the obtained plants of both I. attica and I. pseudopumila. Mixoploidy was detected in root meristem of some of the plants, the incidence of which was 10.9 % for I. pseudopumila and 30.4 % for I. attica. The frequency of cells with chromosomal rearrangements revealed by anaphase analysis in root meristem of I. pseudopumila seedlings was 2.6 %; in I. attica plants, chromosome aberrations were not detected. Conclusions. The plants of I. attica and I. pseudopumila were introduced into in vitro culture, aseptic seedlings were obtained, which showed a high survival level when adapted to greenhouse conditions. Chromosome number 2n = 16 was established for the obtained plants of both species. The root apical meristems of the seedlings were found to be mixoploid, with the incidence of mixoploidy in I. attica identified as three times higher than in I. pseudopumila plants.Keywords: I. attica Boiss. & Heldr., I. pseudopumila Tineo, aseptic seedlings, mixoploidy, anaphase aberration.
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Josephine U Agogbua and Bosa E Okoli. "Procedure for in vitro seed sterilization, germination and aseptic seedling establishment of Zehneria capillacea (Schumach) C. jeffrey." GSC Biological and Pharmaceutical Sciences 19, no. 1 (2022): 143–48. http://dx.doi.org/10.30574/gscbps.2022.19.1.0135.
Full textAbstract:
The most important step in plant tissue culture technique is standardizing a protocol for explant sterilization using phytotoxic sterilants. In this study, two sterilants, sodium hypochlorite (household bleach) and mercuric chloride were evaluated to determine the most suitable concentration and exposure time for explant sterilization of Zehneria capillacea seeds, an undomesticated diploid herbaceous edible cucurbit that is rarely distributed in the Niger Delta region of Nigeria. O.1% mercuric chloride for 1minute and 20% sodium hypochlorite for 15 minutes achieved an efficient (95%) seedling establishment. The study also evaluated the effect of aseptic seed coat removal on in vitro seedling establishment. Seeds with intact coat had 0% germination six weeks after initiation into Murashige and Skoog (MS) medium while all the decorticated seeds showed 100% germination with cotyledonary growth and roots one week after initiation, indicating that the seed coat is a major barrier to the in vitro germination of this species. It was also observed that seed orientation during culture initiation influenced seedling vigor and growth. Three weeks after culture initiation, the aseptic seedlings generated from decorticated seeds were observed to have grown 4-5 cm in height with formation of tendrils, apical and axillary nodes, which could be used as explant source for various in vitro culture studies such as callus induction, shoot multiplication, in vitro polyploidization and production of secondary metabolites.
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Josephine, U. Agogbua, and E. Okoli Bosa. "Procedure for in vitro seed sterilization, germination and aseptic seedling establishment of Zehneria capillacea (Schumach) C. jeffrey." GSC Biological and Pharmaceutical Sciences 19, no. 1 (2022): 143–48. https://doi.org/10.5281/zenodo.6624268.
Full textAbstract:
The most important step in plant tissue culture technique is standardizing a protocol for explant sterilization using phytotoxic sterilants. In this study, two sterilants, sodium hypochlorite (household bleach) and mercuric chloride were evaluated to determine the most suitable concentration and exposure time for explant sterilization of <em>Zehneria capillacea</em> seeds, an undomesticated diploid herbaceous edible cucurbit that is rarely distributed in the Niger Delta region of Nigeria. O.1% mercuric chloride for 1minute and 20% sodium hypochlorite for 15 minutes achieved an efficient (95%) seedling establishment. The study also evaluated the effect of aseptic seed coat removal on <em>in vitro</em> seedling establishment. Seeds with intact coat had 0% germination six weeks after initiation into Murashige and Skoog (MS) medium while all the decorticated seeds showed 100% germination with cotyledonary growth and roots one week after initiation, indicating that the seed coat is a major barrier to the <em>in vitro</em> germination of this species. It was also observed that seed orientation during culture initiation influenced seedling vigor and growth. Three weeks after culture initiation, the aseptic seedlings generated from decorticated seeds were observed to have grown 4-5 cm in height with formation of tendrils, apical and axillary nodes, which could be used as explant source for various <em>in vitro</em> culture studies such as callus induction, shoot multiplication, <em>in vitro</em> polyploidization and production of secondary metabolites.
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Varimadugu, Aruna, and Pullaiah Thammineni. "Organogenesis of Abelmoschus moschatus Medik. Using Aseptic Seedling Explants." European Journal of Medicinal Plants 25, no. 1 (2018): 1–8. http://dx.doi.org/10.9734/ejmp/2018/42720.
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Thomson, J., U. Matthes-Sears, and R. L. Peterson. "Effects of seed provenance and mycorrhizal fungi on early seedling growth in Piceamariana." Canadian Journal of Forest Research 20, no. 11 (1990): 1739–45. http://dx.doi.org/10.1139/x90-231.
Full textAbstract:
The importance of provenance and fungal species effects on mycorrhiza formation in Piceamariana was determined. Seed was collected from two sites near Chapleau, Ontario. One provenance was collected from an upland site with sandy soil, the second from a lowland site with peaty soil. Seedlings were grown under aseptic conditions in test tubes. Two experiments were performed: in the first, seedlings were inoculated with Hebelomacylindrosporum, Laccarialaccata, or Paxillusinvolutus, whereas in the second, seedlings were inoculated with Laccariabicolor, Laccarialaccata, Laccariaproximo or noninoculated agar plugs. Counts of ectomycorrhiza and lateral root formation were analyzed for significance with two-way factorial ANOVAS. When inoculated with L. laccata, seedlings from the lowland seed source had significantly more mycorrhizal second order lateral roots than seedlings from the upland seed source. Lowland seedlings also had greater shoot dry weight and foliage length values. However, overall differences between provenances were small. In contrast, differences between fungal treatments were pronounced. Both L. laccata and L. bicolor initiated good ectomycorrhiza formation and increased seedling growth.
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Mi, Ze-yuan, Ding-hao Lv, Guang-hui Jiang, Jun-feng Niu, Shi-qiang Wang, and Zhe-zhi Wang. "Establishment of a Rapid Breeding System for Bletilla striata." HortScience 56, no. 4 (2021): 454–59. http://dx.doi.org/10.21273/hortsci15598-20.
Full textAbstract:
Bletilla striata (Thunb. ex A. Murray) Rchb. f., a species of perennial herb of orchidaceae that has remarkable effects and high economic value, has been intensively studied by many scholars. Although this herb has many seeds, the germination rate is exceptionally low, which leads to decreased germplasm resources and increased market demand every year. To solve this problem, this study examined the aseptic germination system and the direct seeding technology system. On Murashige and Skoog (MS) medium, 2.0 mg/L 6-benzylaminopurine (6-BA) and 1.0 mg/L naphthylacetic acid (NAA) were added before seed germination, and 70 g/L banana juice and 0.5 mg/L NAA were added when rooting. Then, the seedlings were transplanted to a mixed substrate of humus, river sand, and bark (volume ratio of 3:1:1). The direct seeding system consists of substrate treatment, sowing, seedling raising, seedling growth, and transplanting. Turfy soil, Huangjiang residue, and river sand were selected as the substrate. The results revealed that the germination rate was increased to 91.8%, whereas the plantlet regeneration was increased to 82.0%. After 180 days of cultivation, the plants could be transplanted as finished seedlings. The establishment of B. striata seedling system provides a safe, rapid, reliable production technology route for industrial development.
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Shaik, Sadiq Pasha, and Pious Thomas. "In Vitro Activation of Seed-Transmitted Cultivation-Recalcitrant Endophytic Bacteria in Tomato and Host–Endophyte Mutualism." Microorganisms 7, no. 5 (2019): 132. http://dx.doi.org/10.3390/microorganisms7050132.
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This study was aimed at exploring seed transmission of endophytic bacteria in tomato utilizing aseptic in vitro conditions. Cultivation-based studies were undertaken on two tomato cultivars “Arka Vikas” and “Arka Abha” employing surface sterilized seeds, aseptically germinated seeds and in vitro grown seedlings at different stages. Bacillus sp. appeared primarily as seed externally-associated bacteria. Tissue homogenate from extensively surface-sterilized seeds, day-3 germinating seeds, or 10-day in vitro seedlings did not show any cultivable bacteria on two bacteriological media. Indexing of 4-week old healthy seedlings with seed-coat removal following seed germination showed bacterial association in 50–75% seedlings yielding 106–107 cfu g−1 tissues. Four endophytic bacteria appeared common to both cultivars (Kosakonia, Ralstonia, Sphingomonas, Sphingobium spp.) with three additional species in “Arka Abha”. The bacterial strains showed a manifold increase in growth with host-tissue-extract supplementation. Seed inoculations with single-isolates stimulated germination or enhanced the seedling growth coupled with the activation of additional endophytic bacteria. In vitro seedlings upon recurrent medium-indexing over eight weeks showed gradual emergence of endophytic bacteria. The study reveals the seed internal colonization by different bacterial endophytes in a cultivation-recalcitrant form, their activation to cultivable state during seedling growth and transmission to seedlings with mutualistic effects.
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Jumpponen, Ari, and James M. Trappe. "Performance of Pinus contorta inoculated with two strains of root endophytic fungus, Phialocephala fortinii: effects of synthesis system and glucose concentration." Canadian Journal of Botany 76, no. 7 (1998): 1205–13. http://dx.doi.org/10.1139/b98-098.
Full textAbstract:
Melanized, root-colonizing fungi are ubiquitous. Their ecological role, however, is poorly understood, and results of studies of associations between these fungi and their potential host plants are controversial. The culture system under which the association is studied may also affect the host-fungus interaction. Two experiments on the association between Pinus contorta Dougl. ex Loud., and a root-inhabiting endophytic fungus, Phialocephala fortinii Wang & Wilcox, were conducted to study the host response to inoculation. First, Pinus contorta seedlings were inoculated with two strains of Phialocephala fortinii and grown under aseptic conditions with five levels of glucose in the medium. Multiple linear regression analysis was employed to study the effects of inoculation and glucose concentration. Second, the same two strains of Phialocephala fortinii were inoculated on Pinus contorta seedlings in open pot cultures. Inoculation resulted in substantial increase in all biomass components of the host plant in the aseptic culture system. Total biomass, for example, was increased approximately 60 and 90% when seedlings were inoculated with strain 1 and strain 2, respectively. No seedling mortality was observed following fungal inoculation after 6 months of incubation. Inoculation increased host biomass with increasing glucose concentration, while glucose concentration did not significantly affect host biomass when no inoculum was added. Inoculation lowered foliar nitrogen and phosphorus concentrations. The effect of glucose concentration on the foliar nutrient concentrations varied between the two strains. In the open pot cultures, inoculation did not affect biomass or foliar nutrient concentration. We hypothesise that the observed increases in host growth in the aseptic culture system are due to fungal respiration in a closed culture system, the carbohydrates made available to the host plant by the fungus, or, most likely, to a combination of both factors. The ecological role of Phialocephala fortinii and the validity of aseptic culture assays are discussed.Key words: dark-septate endophytes, deuteromycetes, fungi, host-fungus associations, root endophytes, symbiosis.
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YILMAZ GÖKDOĞAN, Emel, Müge ETİK, and Betül BÜRÜN. "Aseptic Seedling Production by In Vitro Seed Culture in Salvia fruticosa Mill." Ordu Üniversitesi Bilim ve Teknoloji Dergisi 12, no. 1 (2022): 11–17. http://dx.doi.org/10.54370/ordubtd.1028243.
Full textAbstract:
Lamiaceae familyası üyelerinden olan Salvia cinsi tıbbi ve aromatik olarak önemli bir cinstir. Ülkemizde yetişen ve Anadolu adaçayı olarak isimlendirilen Salvia fruticosa Mill. türünün uçucu yağ içeriğinin diğer türlere göre daha yüksek olması, doğadan toplanarak ticaretinin yapılmasına ve ekonomik değerinin artmasına neden olmaktadır. Bu çalışmada Salvia fruticosa tohumlarının 1/10 Murashige-Skoog (MS) (1962) besin ortamında çimlendirilmesi ile in vitro kültürlerde yararlanmak üzere aseptik fide elde edilmesi araştırılmıştır. Kültür öncesi tohumlar %70 etil alkole 2 dakika batırılmış ve takiben NaOCl (klor), H2O2 (oksidan) ve H2SO4 (asit) ile muamele edilmiştir. Bu tohumlar üç gruba ayrılmış: 1. ve 2. grup tohumlara soğuk uygulaması yapılmamış, 3. gruba soğuk uygulaması yapılmıştır. 1. grup tohumlar 1 hafta karanlığı takiben fotoperiyodik koşullara alınırken; 2. ve 3. gruptaki tohumlar fotoperiyodik koşulda tutulmuşlardır. En yüksek çimlenme %50’dir ve 3 dakika %30-31 H2O2 ile yüzeysel sterilizasyonu yapılmış tohumların karanlığı takiben fotoperiyodik koşuldaki kültüründen elde edilmiştir. Aynı koşuldaki tohumların filtre kâğıdı üzerindeki çimlenme testinde (kontrol) ise çimlenme %48 olmuştur. Soğuk uygulama yapılan ve fotoperiyodik koşulda tutulan tohumlarda ise enfeksiyon, düşük gözlenmiştir. Sonuç olarak, Salvia fruticosa’nın farklı amaca yönelik in vitro kültürlerinde eksplant olarak kullanılabilecek steril fidelerin üretimi için en yüksek çimlenme yüzdesini (%50) veren H2O2’nin kullanıldığı prosedür uygulanabilir özelliktedir.
Dissertations / Theses on the topic "Aseptic Seedling"
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Cheng, Yu-Ping, and 鄭玉苹. "Morphological Comparison of Semi-aseptic and Nature-infected White Ectomycorrhizae on Cyclobalanopsis glauca (Thund.) Oerst Seedlings." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/63219560370205447887.
Full textAbstract:
碩士<br>國立臺灣大學<br>森林學系研究所<br>86<br>ABSTRACT
The purpose of this study is to observe the characteristics
of ectomycorrhizae synthesis by semi-aseptic cultures with
Boletus edulis 002 ,Macrolepiota procera 001 and Scleroderma
areolatum 001 with Cyclobalanopsisglauca seedling and to
compare their morphological difference with thenature-infected
whiteectomycorrhizal of C. glauca seedling grown in
thegreenhouse.
By the semi-aseptic synthetic culture , all of B. edulis
002 , M. procera 001 and S. areolatum 001 could form typical
white ectomycorrhizae with C.glauca seedling
These semi-aseptic C.glauca ectomycorrhizae and the Natuer-
infected white ectomycorrhizae could not be distinguished by
naked eye.So,for the basis of classfication, they were
morphologically investigated under stereomicroscope , light
microscope , scanning electron microscope. Besides, the
reactions of mycorrhizal elements to the chemical reagents were
tested respectively.
The Characteristics of C. glauca ectomycorrhizae formed in this
experiment could be described as follows:
1. According to the microscopic obserrvation , only the
emanating hypha of M. procera 001 has clamp connection ,
the other mycorrhizal types do not form clamp connection.
2. Harting nets for med by B. edulis 002 and S. areolatum 001
reached to the 2nd or 3rd layer of cortex cells, but those
of M. procera 001 and the nature-infected white
ectomycorrhizae formed only reached to the 1st or 2nd
layer of cortex cells.
3. The structure of dolipore septum is different among the
four mycorrhizal types. The pore cap of all species is
disconnected. The pore diameter of M. procera 001 is the
smallest and the part of the dolipore septum is not
swollen. The shape of dolipore septum of B. edulis 002 is
onion-like.The dolipore formed by S. areolatum 001 and that
of nature-infected mycorrhiza is pea- shaped
and creascort-shaped,respectively.
4. According to the reactions of the mycorrhical elements to
the chemical reagents , the four ectomycorrhizal types
can also be classified by 37﹪ formalin、2﹪phenol、conc.
NH4OH、15﹪KOH and guaiacol.
Key words ： ectomycorrhizae, semi-aseptic synthesis , Boletus
edulis, Macrolepiota procera , Scleroderma
areolatum , dolipore spectum
Conference papers on the topic "Aseptic Seedling"
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Asadova, S. Sh. "Introduction of Stevia rebaudiana Bertoni in in vitro culture." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.294.
Full textAbstract:
A cell culture obtained from explants of adult plants and aseptic seedlings of the Stevia rebaudiana Bertoni variety with different levels of ploidy, characterized by high speed, proliferation and ability to morphogenesis.