Journal articles: 'Aseptic techniques'

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Merry, Alan F., Derryn A. Gargiulo, Janie Sheridan, Craig S. Webster, Simon Swift, Jane Torrie, Jennifer Weller, Kaylene Henderson, and Jacqueline A. Hannam. "Incorrect representation of aseptic techniques." European Journal of Hospital Pharmacy 24, no. 3 (January 6, 2017): 192. http://dx.doi.org/10.1136/ejhpharm-2016-001174.

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Male, Emma. "Aseptic techniques in dental implantology." Dental Nursing 14, no. 3 (March 2, 2018): 148–49. http://dx.doi.org/10.12968/denn.2018.14.3.148.

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Virtanen, Sonja, Karmen Kapp, Maria Rautamo, Lotta Schepel, Carita Lindén-Lahti, Cristina D. Cruz, and Päivi Tammela. "Compounding Parenteral Products in Pediatric Wards—Effect of Environment and Aseptic Technique on Product Sterility." Healthcare 9, no. 8 (August 10, 2021): 1025. http://dx.doi.org/10.3390/healthcare9081025.

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Parenteral products must be compounded using an aseptic technique to ensure sterility of the medicine. We compared the effect of three clinical environments as compounding areas as well as different aseptic techniques on the sterility of the compounded parenteral product. Clinical pharmacists and pediatric nurses compounded 220 samples in total in three clinical environments: a patient room, a medicine room and biological safety cabinet. The study combined four methods: observation, environmental monitoring (settle plates), monitoring of personnel (finger dab plates) and sterility testing (membrane filtration). Of the compounded samples, 99% were sterile and no significant differences emerged between the clinical environments. Based on the settle plates, the biological safety cabinet was the only area that fulfilled the requirements for eliminating microbial contamination. Most of the steps on the observation form for aseptic techniques were followed. All participants disinfected their hands, wore gloves and disinfected the septum of the vial. Non-contaminated finger dab plates were mostly detected after compounding in the biological safety cabinet. Aseptic techniques were followed relatively well in all environments. However, these results emphasize the importance of good aseptic techniques and support the recommendation of compounding parenteral products in biological safety cabinets in clinical environments.

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Ikenganyia, E., M. Anikwe, T. Omeje, and J. Adinde. "Plant Tissue Culture Regeneration and Aseptic Techniques." Asian Journal of Biotechnology and Bioresource Technology 1, no. 3 (January 10, 2017): 1–6. http://dx.doi.org/10.9734/ajb2t/2017/31724.

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Kleinbeck, Susan. "Infractions in aseptic techniques: A reasearch study." AORN Journal 44, no. 1 (July 1986): 24. http://dx.doi.org/10.1016/s0001-2092(07)65196-5.

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Klinger, Donna L. "Basic Aseptic Techniques in the Operating Room." AORN Journal 55, no. 1 (January 1992): 12. http://dx.doi.org/10.1016/s0001-2092(07)69041-3.

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Rowley, Stephen, and Simon Clare. "How widely has ANTT been adopted in NHS hospitals and community care organisations in England and Scotland?" British Journal of Nursing 29, no. 16 (September 10, 2020): 924–32. http://dx.doi.org/10.12968/bjon.2020.29.16.924.

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Background: To the detriment of patient safety, the important clinical competency of aseptic technique has been notoriously variable in practice, and described ambiguously in the literature, internationally. From a UK perspective, attempts have been made to improve patient safety by reducing variability and improving education and practice through standardisation. The Welsh Government mandated Aseptic Non Touch Technique (ANTT®) as a specific national standard in 2015. All healthcare organisations in England are required by the Health and Social Care Act 2008 to have a single standard aseptic technique, demonstrable by the clinical governance indicators of education, training, competency assessment and compliance audit. In Scotland, an education-based initiative was launched by NHS Education for Scotland in 2012. To review the impact of these and other initiatives on the current status of aseptic technique, all NHS trusts in England and NHS health boards in Scotland were assessed under the Freedom of Information procedure. Findings: 93% of NHS trusts in England use a single standard for aseptic technique. In 88% of these trusts the single standard was stipulated as being ANTT. In Scotland, 62% of NHS acute and community care hospitals within health boards use a single standard. In 56% of these, the single standard was ANTT. When including those that use ANTT in combination with other techniques ANTT usage is 73%. Conclusion: These data demonstrate significant progress in standardising aseptic technique education, assessment and governance, and confirms ANTT as the de facto aseptic technique used in NHS trusts in England and health boards in Scotland.

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Rowley, Nicole L., Elliot Ramos-Rivera, Sorana Raiciulescu, Sang H. Lee, and Amanda C. Christy. "Comparison of Two Hair Removal Methods in Sprague–Dawley Rats (Rattus norvegicus)." Journal of the American Association for Laboratory Animal Science 60, no. 2 (March 1, 2021): 213–20. http://dx.doi.org/10.30802/aalas-jaalas-20-000108.

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Rats commonly undergo surgery for research purposes. However, the effects of different methods of hair removal on wound healing and surgical site infections (SSI) in rats has not been evaluated. The current study evaluated 2 hair removal methods, clipping with an electric clipper and using a depilatory agent, and their effect on wound healing and SSI. Swabs for bacterial culture were obtained on Day 0 just after hair removal, after aseptic skin preparation, and on Days 1 and 3 before conducting skin biopsies to assess bacterial load and recolonization. Full-thickness punch biopsies were taken for histopathologic evaluation on Days 0, 1, 3, 7, and 10. The surgical incisions were assigned an ASEPSIS score on Days 1 and 3. The data revealed that the bacterial load was significantly higher with the depilatory method as compared with the clipper method, but only on Day 1. The histopathologic evaluation found no significant difference in wound healing between the 2 methods. Although the ASEPSIS score was significantly higher for the clipping method than for the depilatory method on Day 1, both techniques were equivalent by Day 3. We conclude that both hair removal methods are safe and efficacious components of aseptic technique in rats.

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Dziewa, Agnieszka, Anna Ksykiewicz-Dorota, Marek Kos, and Bartłomiej Drop. "Nurse care quality and hospital-acquired infections: adhering to aseptic techniques." Polish Journal of Public Health 125, no. 3 (September 1, 2015): 133–36. http://dx.doi.org/10.1515/pjph-2015-0040.

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Abstract Introduction. The quality of medical services can be regarded as an indication of the changes being implemented at a given moment. At the same time, improving the care quality remains essential, regardless of the current situation. This makes upgrading employees’ skills a necessity, for instance by preventing undesired events, like hospital acquired infections which are quite common. Aim. The aim of this study was to measure the quality of nursing care delivered to patients who contracted a hospital-acquired disease, with special attention paid to the adherence by aseptic procedures. Material and methods. The authors of this research study want to assess the quality of nursing care, looking through the lens of adherence by aseptic techniques. Results. The study was conducted in hospitals of three different referral levels. Both the highest (=95.7%) and the lowest (=84.7%) rates in the field of quality of nursing care were reported in provincial hospitals. Conclusion. 1. There is a 10% deficit in terms of adherence to aseptic procedures. 2. Constant supervision and upgrading nursing staff skills is essential.

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Rowley, Stephen, and Simon Clare. "Standardizing the Critical Clinical Competency of Aseptic, Sterile, and Clean Techniques with a Single International Standard: Aseptic Non Touch Technique (ANTT®)." Journal of the Association for Vascular Access 24, no. 4 (December 1, 2019): 12–17. http://dx.doi.org/10.2309/j.java.2019.004.003.

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HEBL, J. "The Importance and Implications of Aseptic Techniques During Regional Anesthesia." Regional Anesthesia and Pain Medicine 31, no. 4 (July 2006): 311–23. http://dx.doi.org/10.1016/j.rapm.2006.04.004.

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Hebl, James R. "The Importance and Implications of Aseptic Techniques During Regional Anesthesia." Regional Anesthesia and Pain Medicine 31, no. 4 (July 2006): 311–23. http://dx.doi.org/10.1097/00115550-200607000-00006.

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Drezner, Kate, Mike Antwi, Paula Del Rosso, Marie Dorsinville, Pamela Kellner, and Joel Ackelsberg. "A Cluster of Methicillin-Susceptible Staphylococcus aureus Infections at a Rheumatology Practice, New York City, 2011." Infection Control & Hospital Epidemiology 35, no. 2 (February 2014): 187–89. http://dx.doi.org/10.1086/674850.

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A cluster of 5 methicillin-susceptible Staphylococcus aureus infections occurred after administration of methylprednisolone acetate injections in a rheumatology practice. A site visit was conducted to inspect examination rooms, observe techniques, and review charts. The investigation revealed a pervasive lack of aseptic technique that led to multiple opportunities for medication contamination.

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Al-Abboodi, Hasan H. "Evaluation Aseptic Loosening of Primary Hip Arthroplasty." AL-Kindy College Medical Journal 16, no. 1 (September 5, 2020): 30–37. http://dx.doi.org/10.47723/kcmj.v16i1.187.

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Background : The number of primary hip arthroplasty is growing with every passing year. At present this surgical operation has no equal in the rate of yielding good results. Despite the fact that in many cases in the long term after surgery radiolucent lines around the cup are observed, the clinical outcomes remain satisfactory.Aim of the study :To study the clinical and radiological manifestations of the aseptic loosening of the endoprosthesisMethods: This is a prospective study based on an analysis of diagnostic findings of 51 patients with aseptic loosening of hip joint components, aged 30 to 84 years. We depend for diagnosis of a septic losing by both clinical evaluation and X-ray assessment Results:The majority of clinical and radiological evidence of aseptic loosening appear after three years (47%).All our patients had a pain syndrome of varying intensity, with (43.2%) used a walking-stick. Shortening limb from 1 to 7 cm was found in 43 patients 85.2%.The aseptic losing are common in cementless hip prosthesis especially in femoral stem part of prosthesus 53.9% versus 25.6% in cemented type,Revision surgery for primary hip prosthesis occur mainly for femoral stem involve 49% while other 25.5% involve both acetabular cup and femoral stem, while the rest of patient(25.5%) involve revision of acetabular cup only. Discussion :At present this surgical operation has no equal in the rate of yielding good results. The lifetime of the majority of artificial joints does not exceed 15 years mainly due to aseptic loosening of one or both endoprosthetic components There are many causes of aseptic instability: substandard surgical technique, unwarranted extension of the indications for arthroplasty, inappropriate choice of the prosthesis dimension type, incorrect installation of components, reaction to the massive foreign body and the development of synovial-like membrane on the metal-bone interface .As a rule, early instability within 1 year was associated with defects in the operative techniques, from 1 to 3 years mostly due to the wrong selection of the type of primary endoprosthesis while more than 3 years period, the instability was caused by two reasons: excessive load on the operated extremity or traumas and the rapid wear of plastic with large areas of osteolysis due to the high loads or inaccurate positioning of the prosthetic cup.Conclusions: X-ray examination is the fundamental for diagnosing of aseptic loosening of hip endoprosthesis , and improved surgical techniques, the proper selection of the type of prosthesis are the keys for reduction of risk of aseptic loosening

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Tejwani, Samir G., Heather A. Prentice, Ronald W. B. Wyatt, and Gregory B. Maletis. "Femoral Tunnel Drilling Method: Risk of Reoperation and Revision After Anterior Cruciate Ligament Reconstruction." American Journal of Sports Medicine 46, no. 14 (November 12, 2018): 3378–84. http://dx.doi.org/10.1177/0363546518805086.

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Background: The femoral tunnel in anterior cruciate ligament reconstruction (ACLR) can be created by the transtibial (TT) or tibial-independent (TI) methods. An anatomically located femoral tunnel can be more consistently achieved by TI methods, which include the anteromedial portal and lateral (outside-in, retrodrill) techniques. Nonanatomic graft placement in ACLR can result in postoperative instability and meniscal or chondral injury. An anatomically located graft is subjected to higher postoperative physiologic forces than one placed nonanatomically. Purpose: To examine isolated primary ACLR and determine the risk of aseptic revision and reoperation based on femoral tunnel drilling method. Study Design: Cohort study; Level of evidence, 2. Methods: The ACLR registry of an integrated US health care system was used to identify primary isolated unilateral ACLRs from 2009 to 2014. Multivariable Cox proportional hazard regression models were used to evaluate risk for aseptic revision for graft failure and aseptic reoperation for meniscal or chondral injury according to femoral tunnel drilling method: TI versus TT. Models included age, sex, body mass index (BMI), race, graft type, and femoral fixation type as covariates. Results: The cohort included 19,059 patients with primary ACLR. The mean age was 28.9 years (SD, 11.5), 6991 patients (36.8%) were younger than 22 years, 11,795 patients (61.9%) were male, 7648 patients (40.1%) had a BMI less than 25 kg/m2, 8913 patients (46.8%) were white, and 7357 patients (38.6%) received an allograft. Median follow-up was 2.30 years (interquartile range, 1.08-3.77). TI techniques were used for 12,342 (64.8%) of the ACLRs, and the TT method was used for 6717 (35.2%). Use of TI techniques increased from 33.6% of all ACLRs in 2009 to 83.4% in 2014. After adjustment for covariates, the TI group had a higher risk for aseptic revision than the TT group (hazard ratio [HR], 1.28; 95% CI, 1.04-1.56), and this risk was 1.41 times higher in patients younger than 22 years specifically. The 5-year cumulative reoperation probability was lower in the TI group (4.50%; 95% CI, 3.78%-5.36%) compared with the TT group (5.06%; 95% CI, 4.31-5.94%). After adjustment for the covariates, no difference in risk for aseptic reoperation was observed (HR, 1.08; 95% CI, 0.85-1.39). Conclusion: In the largest known study of its type examining femoral tunnel drilling method for primary ACLR, after adjustment for age, sex, BMI, race, graft type, and femoral fixation, TI techniques were found to carry higher risk of aseptic revision compared with the TT method, while no difference was observed in risk for aseptic reoperation.

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Isadiartuti, Dewi, Sugiyartono Sugiyartono, Retno Sari, Muh Agus S. Rijal, and Dini Retnowati. "EDUKASI TEHNIK ASEPTIK SEDIAAN STERIL BAGI TENAGA KESEHATAN DI FASILITAS PELAYANAN KESEHATAN KABUPATEN SUMBA TIMUR NTT." Jurnal Layanan Masyarakat (Journal of Public Services) 4, no. 1 (June 29, 2020): 237. http://dx.doi.org/10.20473/jlm.v4i1.2020.237-242.

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The Government through Permenkes 72/2016 on Pharmaceutical Services Standards in Hospitals has provided guidance on the regulation of pharmaceutical service standards. Pharmaceutical services in the field of management of sterile preparations is one aspect that receives attention, because it will affect the quality of services provided to sufferers. East Sumba Regency is one of the East Nusa Tenggara provinces located in Eastern Indonesia. East Sumba Regency has a large enough population but health workers in health care facilities have limitations in getting the latest information. To answer this condition, training was held in collaboration with IAI PC East Sumba. The aim of the training is to increase the knowledge and skills of aseptic techniques for sterile preparations for health workers in health care facilities so that the quality of health services can be improved. The training was given in the form of lectures consisting of compatibility and stability material for parenteral preparations and dispensing materials for sterile preparations followed by the practice of aseptic techniques for sterile preparations. The training activity was attended by 49 participants consisting of pharmacists, nurses, midwives and pharmaceutical technical personnel who worked in hospitals, health services, health centers, clinics, and pharmacies in Sumba (East, Central and West). From the results of the evaluation of the activity, it was known that the participants gained knowledge and increased knowledge about the basic principles of aseptic techniques and there was an increase in the participants' understanding of the material provided.abstrakPemerintah melalui Permenkes 72/2016 tentang Standar Pelayanan Kefarmasian di Rumah Sakit telah memberikan arahan mengenai pengaturan standar pelayanan kefarmasian. Pelayanan kefarmasian di bidang pengelolaan sediaan steril merupakan salah satu aspek yang mendapat perhatian, karena akan memengaruhi mutu pelayanan yang diberikan kepada penderita. Kabupaten Sumba Timur merupakan salah satu wilayah propinsi Nusa Tenggara Timur yang terletak di Indonesia Bagian Timur. Kabupaten Sumba Timur memiliki jumlah penduduk yang cukup besar akan tetapi tenaga kesehatan di fasilitas pelayanan kesehatan memiliki keterbatasan dalam mendapatkan informasi terbaru. Untuk menjawab kondisi tersebut diadakan pelatihan bekerjasama dengan IAI PC Sumba Timur. Tujuan pelatihan adalah untuk meningkatkan pengetahuan dan ketrampilan tehnik aseptik sediaan steril bagi tenaga kesehatan di fasilitas pelayanan kesehatan agar mutu pelayanan kesehatan dapat meningkat. Pelatihan diberikan dalam bentuk ceramah terdiri dari materi kompatibilitas dan stabilitas sediaan parenteral dan materi dispensing sediaan steril dilanjutkan dengan praktek tehnik aseptik sediaan steril. Kegiatan pelatihan diikuti 49 peserta yang terdiri dari apoteker, perawat, bidan dan tenaga teknis kefarmasian yang bekerja di Rumah Sakit, Dinas Kesehatan, Puskesmas, Klinik, dan Apotek sedaratan Sumba (Timur, Tengah, dan Barat). Dari hasil evaluasi kegiatan diketahui peserta mendapatkan penyegaran ilmu dan peningkatan wawasan tentang prinsip dasar tehnik aseptik dan terdapat peningkatan pemahaman peserta terhadap materi yang diberikan.

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Shettigar, Savithri, Abhishek Somasekhara Aradhya, Srinath Ramappa, Venugopal Reddy, and Praveen Venkatagiri. "Reducing healthcare-associated infections by improving compliance to aseptic non-touch technique in intravenous line maintenance: a quality improvement approach." BMJ Open Quality 10, Suppl 1 (July 2021): e001394. http://dx.doi.org/10.1136/bmjoq-2021-001394.

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BackgroundLack of standardisation and failure to maintain aseptic techniques during procedures contributes to healthcare-associated infections (HCAI). Although numerous procedures are performed in neonatal intensive care units (NICU), handling peripheral intravenous lines is one of the simple and common procedures performed daily. Despite evidence-based care bundle approach variability is higher, and compliance to asepsis is less in routine clinical practice. In this study, we aimed to standardise and improve compliance with Aseptic non-technique (ANTT) in intravenous line maintenance of neonates admitted to NICU to reduce HCAI by 50% over 6 months.MethodsAll nurses were subjects of assessment for compliance with intravenous line maintenance. All admitted neonates with intravenous lines were subjects for the HCAI data collection. At baseline, the current practices for intravenous line maintenance were observed on a generic ANTT audit proforma. Pictorial standard operating procedure (SOP) was developed based on ANTT. Implementation and sustenance were ensured by Plan-Do-Study-Act cycles. Audit data on compliance to ANTT and trends of HCAI rates were displayed using run charts monthly. Qualitative experience from the nursing staff was also recorded.ResultsSignificant improvement was seen in compliance to various components—use of the aseptic field (0% to 100%), closed ports (0% to 100%), key part contamination reduction (80% to 0%), and intravenous hub scrubbing (0% to 72%). SOP of intravenous line maintenance based on ANTT could be implemented and sustained throughout for 9 months. There was a reduction of HCAI from 26 per 1000 patient days to 8 per 1000 patient days. Qualitative experience showed the main determinant of compliance to scrub the hub was the neonate’s sickness level.ConclusionsUsing a quality improvement model of improvement, ANTT in intravenous line maintenance was implemented stepwise. Improving compliance with ANTT principles in intravenous line maintenance reduced HCAI. Scrub the hub requires longer sustained efforts to become part of the practice.

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Gililland, Jeremy M., Lucas A. Anderson, Jill Erickson, Christopher E. Pelt, and Christopher L. Peters. "Mean 5-Year Clinical and Radiographic Outcomes of Cementless Total Hip Arthroplasty in Patients under the Age of 30." BioMed Research International 2013 (2013): 1–7. http://dx.doi.org/10.1155/2013/649506.

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We performed a retrospective review of 40 consecutive modern cementless THAs with 65-month mean followup in 34 patients under the age of 30 primarily for diagnoses other than inflammatory arthritis. We found acceptable functional improvement and radiographic outcomes at mean 5-year followup. We found a high transfusion rate, dislocation rate (10%), and midterm overall aseptic revision rate (17%). Twenty-eight (67.5%) of hips in this series were metal on metal, with a large percentage of aseptic revisions related to metallosis (57%). When revisions due to metallosis were excluded, the aseptic revision rate was 7.5%. The high prevalence of prior pediatric hip surgery in these patients (50%) may predispose to increased technical difficulty resulting in increased complications and higher revision rates. Although our revision rate was high in these young patients, it is favorable compared to older techniques and consistent with the limited data available with modern cementless techniques in patients of similar age. Cementless THA with modern designs remains a viable option for the treatment of arthritis in the young patient.

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Kempthorne, J. T., R. Ailabouni, S. Raniga, D. Hammer, and G. Hooper. "Occult Infection in Aseptic Joint Loosening and the Diagnostic Role of Implant Sonication." BioMed Research International 2015 (2015): 1–8. http://dx.doi.org/10.1155/2015/946215.

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Our aim was to determine the incidence of occult infection and to examine the role of ultrasound sonication of the implants in cases of presumed aseptic loosening in a prospective trial. Joint swabs, aspirates, and deep tissue samples were obtained from around the prosthesis for routine microbiology. Each prosthesis was sonicated and the sonicate examined with Gram staining and extended cultures. There were 106 joints in the study of which 54 were revised for aseptic loosening and 52 were assigned to the control revision group. There were 9 positive cultures with 8/54 positive cultures in the aseptic loosening group and 1/52 in the control revision group (p=0.017, associated OR 47.7). We found concordant results between sonication fluid culture and conventional samples in 5/9 cultures. Preoperative inflammatory markers were not prognostic for infection.Coagulase-negative Staphylococcuswas the most commonly cultured organism (7/9). Previously unrecognised infection was present in 15% of patients undergoing revision for aseptic loosening. Ultrasound sonication of the removed prosthesis was less sensitive than conventional sampling techniques. We recommend routine intraoperative sampling for patients having revision for aseptic loosening, but we do not support the routine use of ultrasound sonication for its detection.

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Mathews, Bridgit Anne, Edmond O’loughlin, and Rajanikanth Potula. "Audit of Monitoring and Aseptic Techniques during Ultrasound-guided Peripheral Nerve Blocks." International Journal of Perioperative Ultrasound and Applied Technologies 3 (2014): 1–4. http://dx.doi.org/10.5005/jp-journals-10027-1055.

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Sharma, Navin. "Aseptic germination of oospores of Phytophthora palmivora." Canadian Journal of Botany 68, no. 12 (December 1, 1990): 2548–52. http://dx.doi.org/10.1139/b90-321.

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Oospores of Phytophthora palmivora were harvested from pairings of isolates of A1 and A2 mating types. The oospores were subjected to sonication and treatment with hemicellulase and cellulase before plating onto agar medium. After incubation at 20 °C, germination of the oospores was observed as the emergence of germ tubes which terminated in germ sporangia. The number of oospores that germinated from any pairing was raised by exposing the oospores to a temperature of 40 °C during the enzyme treatment and allowing a maturation period of the oospores of at least 4 weeks. Oospore germination was depressed by chilling to −12 °C during the enzyme treatment. The addition of an extract from papaya foliage did not significantly affect the frequency of oospore germination. Following the techniques described in this study, sexual progeny, freed from contamination by alien organisms or vegetative hyphae of the parent isolates, can be reliably obtained. Key words: Phytophthora palmivora, oospore, germination.

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Hamill, Richard J., Charles E. Wright, Nita Andres, and Maureen A. Koza. "Urinary Tract Infection Following Instrumentation for Urodynamic Testing." Infection Control 10, no. 01 (January 1989): 26–32. http://dx.doi.org/10.1086/645911.

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AbstractAfter identifying a temporal cluster of urinary tract infections in patients who had undergone urodynamic procedures, we examined the techniques within the urodynamic laboratory and retrospectively reviewed charts of all 155 patients tested in the previous six months. The rate of acquired urinary tract infections was 18.7%. Risk factors for infection included undergoing cystometrograms and being subject to the first procedure performed in a day. Technical errors in the performance of the urodynamic studies included failure to completely disassemble the apparatus upon completion of a procedure, failure to use sterile components, and lapses in aseptic technique. Bacteria implicated in the outbreak were isolated from tubing, transducers, and flush solutions. After the institution of appropriate technique, all patients tested in the subsequent six months were followed. The rate of acquired urinary tract infection dropped to 5%. Urodynamic apparatus should be completely disassembled following the completion of a procedure; reassembly using sterile components should occur immediately prior to the next procedure; aseptic technique should be maintained; and patients should undergo routine urine screening before a procedure. Surveillance of urodynamic procedures may reveal correctable flaws in technique.

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MLADENOVA, Z., G. BUTTINELLI, A. DIKOVA, A. STOYANOVA, M. TROYANCHEVA, R. KOMITOVA, M. STOYCHEVA, L. PEKOVA, K. PARMAKOVA, and L. FIORE. "Aseptic meningitis outbreak caused by echovirus 30 in two regions in Bulgaria, May–August 2012." Epidemiology and Infection 142, no. 10 (December 19, 2013): 2159–65. http://dx.doi.org/10.1017/s0950268813003221.

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SUMMARYAn aseptic meningitis outbreak emerged in two regions in Bulgaria in 2012 and echovirus 30 (E30) was established as the aetiological agent by cell culture isolation, serological test, and molecular-based techniques. A total of 157 patients with aseptic meningitis were investigated, of which 117 were confirmed as having E30-associated disease. Molecular analysis of 12 E30 isolates revealed 99–100% nucleotide and amino-acid identity between them and a close correlation with a Greek strain involved in an E30 outbreak in 2012. Children aged 5–14 years were mainly affected, which could reflect the absence of E30 epidemics in Bulgaria for a period of 11 years. The first case with E30 isolation (a 2-year-old patient from Plovdiv) was notified at the end of April 2012. This was most likely the index case, from which the spread of the virus started, causing sporadic cases first, which later led to an aseptic meningitis outbreak facilitated by person-to-person viral transmission.

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Narang, Sagar, and Prakash Sapkota. "Fatal Salmonella Typhi Necrotising Fasciitis following Intra-articular Steroid Injection." Journal of Lumbini Medical College 1, no. 2 (December 30, 2013): 125. http://dx.doi.org/10.22502/jlmc.v1i2.36.

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Intra-articular steroids administration in the absence of aseptic precautions can have disastrous consequences. Immunocompromised patients are at an increased risk of developing infections following such procedures. Salmonella has been infrequently reported as a causative organism for necrotising fasciitis. Gram negative endotoxemia with disseminated intravascular coagulation resulted in fatality in this patient. The case study is being presented to emphasise the need for aseptic precautions and sterile techniques while administering intra-articular steroids, to have a low threshold towards treating early joint infections expeditiously, and to consider possibility of a gram negative organism as a cause of septicaemia and necrotising fasciitis especially in debilitated patients.

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Duggan, P. J., C. J. Burke, S. Saha, M. Moonim, M. George, A. Desai, and R. Houghton. "Current literature and imaging techniques of aseptic lymphocyte-dominated vasculitis-associated lesions (ALVAL)." Clinical Radiology 68, no. 11 (November 2013): 1089–96. http://dx.doi.org/10.1016/j.crad.2013.04.017.

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Bangal, Vidyadhar B. "Work place based assessment of medical interns on surgical asepsis following training in Obstetrics and Gynecology." International Journal of Reproduction, Contraception, Obstetrics and Gynecology 6, no. 12 (November 23, 2017): 5609. http://dx.doi.org/10.18203/2320-1770.ijrcog20175290.

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Background: Internship programme for medical interns is meant to develop basic skills and to have experience in management of common illnesses and emergencies. Medical interns are often posted to the clinical disciplines, without adequate instructions on surgical asepsis and bio-safety measure. Training need was identified for interns in the subject of surgical asepsis.Methods: Hands on training programme was organized for interns that included common procedures performed by them in wards, operation theatre and labour room complex on day to day basis. A pre and post test was performed to assess the gain in knowledge by interns. Subsequently, interns were supervised by residents, nurses and faculty members in labour room, operation theatre and wards. The observations were documented, and immediate feedback was given to interns. Feedback from interns was obtained at the end of the rotation in Obstetrics and Gynecology.Results: There was significant gain in knowledge by interns following training programme. Interns adopted good aseptic precautions during their posting. Compliance to aseptic precautions was significantly more in female interns as compared to male. A demand got generated from other batches of interns in conducting similar training programme for them. Nurses, faculty members and co-ordinator of internship programme gave positive feedback about the changes observed in the batch of interns who underwent training as compared to previous batches.Conclusions: Adoption of techniques of surgical asepsis by interns was improved following training programme held at the beginning of rotation posting in Obstetrics and Gynecology. It is recommended to include the topic in internship orientation programme.

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Randall, Dustin J., Mike B. Anderson, Jeremy M. Gililland, Christopher L. Peters, and Christopher E. Pelt. "A potential need for surgeon consensus: Cementation techniques for total knee arthroplasty in orthopedic implant manufacturers’ guidelines lack consistency." Journal of Orthopaedic Surgery 27, no. 3 (September 1, 2019): 230949901987825. http://dx.doi.org/10.1177/2309499019878258.

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Aim: Given recent concerns regarding the influence of different cements and implants on the rate of aseptic failures in total knee arthroplasty (TKA), we wondered if cementation technique could play a role. The primary aim of this review was to collect and compare the surgeon education materials from eight orthopedic implant manufacturers to evaluate the manufacturers’ recommended cementation technique in TKA and identify if there was any consistency in these readily available guides as to the best practices of cementation of their implants. Materials and Methods: We reviewed contemporary surgeon education guidelines for all TKA systems available from eight manufacturers. Variables included: cement type, batches prepared, surface preparation prior to application, cleaning and/or drying the bone surface, mixing the cement, the waiting phase after the cement has been mixed prior to application, pressurizing the cement, location of cement application, and the curing time. Data were recorded and organized for qualitative comparisons. Results: We identified a total of 43 guides covering 38 implants from eight different manufacturers. There were 41 surgical technique guides and two general brochures regarding cementation techniques available from the manufacturers. Even within the manufacturers’ own guidelines for the different implants, there was a wide variety of differing guidelines on many aspects of the cementation technique. Conclusion: There is clearly no consensus for a preferred cementation technique both within and among manufacturers’ surgeon education materials regarding tibial baseplate cementation during TKA. Efforts may be needed to identify a best-practice cementation technique in an effort to reduce the number of TKA failures associated with aseptic loosening.

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Solod, Eduard Ivanovich, A. F. Lazarev, A. A. Lazarev, Ya G. Gudushauri, M. G. Kakabadze, A. S. Roskidaylo, I. M. Dan, et al. "Potentialities of Surgical Treatment for Acetabular Fractures Using Low-Invasive Techniques." N.N. Priorov Journal of Traumatology and Orthopedics 16, no. 2 (June 15, 2009): 3–9. http://dx.doi.org/10.17816/vto20091623-9.

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Original technique of percutaneous osteosynthesis was applied for the treatment of 24 patients with acetabular columns fractures. The achieved results were compared to the results of osteosynthesis performed using open direct reposition of bone fragments (39 patients). The efficacy of low-invasive surgery in acetabular column fractures with regard to provision of fragments consolidation, prevention of femoral head aseptic necrosis development and achievement of early medical and social rehabilitation of patients was showed. Maintenance of fragments blood supply, use of minimal surgical approach and closed reposition are considered to be the progressive direction of internal osteosynthesis development.

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Sundaram, Kavin, Ahmed Siddiqi, Atul F. Kamath, and Carlos A. Higuera-Rueda. "Trochanteric osteotomy in revision total hip arthroplasty." EFORT Open Reviews 5, no. 8 (August 2020): 477–85. http://dx.doi.org/10.1302/2058-5241.5.190063.

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Trochanteric osteotomy is a technique that allows expanded exposure and access to the femoral canal and acetabulum for a number of indications. There has been renewed interest in variants of this technique, including the trochanteric slide osteotomy (TSO), extended trochanter osteotomy (ETO), and the transfemoral approach, for both septic and aseptic revision total hip arthroplasty (THA). Osteotomy fixation is crucial for achieving union, and wire and cable-plate systems are the most common techniques. TSO involves the creation of a greater trochanter fragment with preserved abductor attachment proximally and vastus lateralis attachment distally. This technique may be particularly useful in the setting of abductor deficiency or when augmented acetabular exposure is needed. ETO is a posterior-laterally based extensile approach that has been successfully utilized for aseptic and septic indications; most series report a greater than 90% rate of union. The transfemoral approach, as known as the Wagner osteotomy, is an extensile femoral approach and is more anterior-based than the alternate posterior-based ETO. It may be particularly useful for anterior-based approaches and anterior femoral remodelling; rates of union after this approach in most reports have been close to 100%. Cite this article: EFORT Open Rev 2020;5:477-485. DOI: 10.1302/2058-5241.5.190063

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Martinez, Alexander A., Juan Castillo, Mirla C. Sanchez, Yamitzel Zaldivar, Yaxelis Mendoza, Maribel Tribaldos, Pablo Acosta, Rebecca E. Smith, and Juan Miguel Pascale. "Molecular diagnosis of echovirus 30 as the etiological agent in an outbreak of aseptic meningitis in Panama: May – June 2008." Journal of Infection in Developing Countries 6, no. 12 (December 15, 2012): 836–41. http://dx.doi.org/10.3855/jidc.2615.

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Introduction: Aseptic meningitis outbreaks are commonly caused by viral pathogens with enterovirus a common etiological agent. Between May and June of 2008, an outbreak of 173 cases of aseptic meningitis occurred in the Chiriqui Province of Panama. Molecular techniques were used to identify the etiological agent. Methodology: Cerebrospinal fluid (CSF) samples from 75 patients were received at the Gorgas Memorial Institute for Health Studies. RNA extraction and one-step RT-PCR were performed on each sample to determine the presence of enterovirus. Thirty-four samples which were positive for enterovirus were subject to group-specific PCR, sequencing, and phylogenetic analysis to identify the etiological agent of the outbreak. Results: The CSF of 58 subjects was found positive for the enterovirus family using RT-PCR. Thirty-four samples were found to belong to the enterovirus B group. Phylogenetic analysis of four successfully sequenced samples revealed echovirus 30 as the etiological agent. Conclusion: Echovirus 30 is reported as the likely cause of an outbreak of aseptic meningitis in Panama, the first since the 1980s.

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Moreschini, O., and F. Margheritini. "A Histological Evaluation of the Bone-Implant Interface Failure in Total Hip Arthroplasty Due to Aseptic Loosening." HIP International 3, no. 2 (April 1993): 46–50. http://dx.doi.org/10.1177/112070009300300203.

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We report a study on how the bone-implant interface of 20 T.H.A. failed because of aseptic loosening. The aim of this study was to look for the mechanism that promotes the activation of the cell-population located in the tissues at the interface. We have demonstrated, with the help of cytochemical and enzymatic techniques, that cell-activation can be supported by similar agents for every type of implant (cemented or cementless). We believe that these agents could be identified with lymphokines, particularly with Interleukin 1 (IL-1), and that the production of these factors is stimulated by mechanical factors. These results evidence the importance of debris, in addition to micromotion, at the beginning of the process of aseptic loosening.

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Suvikas-Peltonen, Eeva, Suvi Hakoinen, Ercan Celikkayalar, Raisa Laaksonen, and Marja Airaksinen. "Incorrect aseptic techniques in medicine preparation and recommendations for safer practices: a systematic review." European Journal of Hospital Pharmacy 24, no. 3 (October 12, 2016): 175–81. http://dx.doi.org/10.1136/ejhpharm-2016-001015.

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Bezzio, Stefania, C. Scolfaro, R. Broglia, R. Calabrese, F. Mignone, P. A. Abruzzese, E. Aidala, et al. "Prospective Incidence Study of Bloodstream Infection in Infants and Children With Central Venous Catheters After Cardiac Surgery in Italy." Infection Control & Hospital Epidemiology 30, no. 7 (July 2009): 698–701. http://dx.doi.org/10.1086/598242.

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This prospective observational study was designed to assess the incidence of, risk factors for, and outcome of catheter-related bloodstream infection in children undergoing cardiac surgery. A staff specifically trained to handle the central venous catheters with proper aseptic techniques and an appropriate patient to medical staff ratio remain the most effective measures to prevent this infection.

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Bridgen, Mark P. "692 Plant Embryo Culture Techniques and Applications." HortScience 35, no. 3 (June 2000): 518C—518. http://dx.doi.org/10.21273/hortsci.35.3.518c.

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Traditional and biotechnological breeding techniques are being united to develop exciting new plants and to improve existing cultivated plants by introducing natural variability from germplasm resources. Intervarietal, interspecific and intergeneric crosses can be accomplished by using plant embryo culture techniques, sometimes also referred to as embryo rescue. Embryo culture involves the isolation and growth of immature or mature zygotic embryos under sterile conditions on an aseptic nutrient medium with the goal of obtaining a viable plant. The technique depends on isolating the embryo without injury, formulating a suitable nutrient medium, and inducing continued embryogenic growth and seedling formation. The culture of immature embryos is used to rescue embryos from hybrid crosses that were once thought to be incompatible because they would normally abort or not undergo the progressive sequence of ontogeny. The culture of mature embryos from ripened seeds is used to eliminate seed germination inhibitors, to overcome dormancy restrictions, or to shorten the breeding cycle. New and exciting cultivars of Alstroemeria, also known as Lily-of-the-Incas, Inca Lily, or Peruvian Lily, have been bred by using zygotic embryo culture; these techniques and applications will be discussed.

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Gorgievski-Hrisoho, Meri, Jean-Daniel Schumacher, Nevenka Vilimonovic, Daniel Germann, and Lukas Matter. "Detection by PCR of Enteroviruses in Cerebrospinal Fluid during a Summer Outbreak of Aseptic Meningitis in Switzerland." Journal of Clinical Microbiology 36, no. 9 (1998): 2408–12. http://dx.doi.org/10.1128/jcm.36.9.2408-2412.1998.

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Enteroviruses (EV) are among the most common causes of aseptic meningitis. Standard diagnostic techniques are often too slow and lack sensitivity to be of clinical relevance. EV RNA can be detected within 5 h by a commercially available reverse transcription-PCR (RT-PCR) test kit. Cerebrospinal fluid (CSF) samples from 68 patients presenting with aseptic meningitis during a summer outbreak in Switzerland were examined in parallel with cell culture and commercial RT-PCR. RT-PCR was positive in all 16 CSF specimens positive by cell culture (100%). In addition, 42 of 52 (80%) CSF samples negative by cell culture were PCR positive. In 26 of these 42 (62%) patients, viral culture from other sites (throat swab or stool) was also positive. The CSF virus culture took 3 to 7 days to become positive. Echovirus 30 was the type most often isolated in this outbreak. The sensitivity of CSF RT-PCR based on clinical diagnosis during this aseptic meningitis outbreak in patients with negative bacterial culture results was 85%, i.e., considerably higher than the sensitivity of CSF virus culture (24%). We conclude that this commercial RT-PCR assay allows a positive diagnosis with minimal delay and may thus influence clinical decisions.

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Mulcahy, Daniel M. "Legal, Ethical, and Procedural Bases for the Use of Aseptic Techniques to Implant Electronic Devices." Journal of Fish and Wildlife Management 4, no. 1 (June 1, 2013): 211–19. http://dx.doi.org/10.3996/092012-jfwm-080.

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Taher, Farida AbdulKader, and Samir Y. Lafi. "Scrub Nurse's Performance Concerning Surgical Aseptic Techniques at the Operating Room of Slemani Teaching Hospitals." Mosul Journal of Nursing 1, no. 1 (January 1, 2015): 1. http://dx.doi.org/10.33899/mjn.2015.160014.

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Herman, Amir, Bassam A. Masri, Clive P. Duncan, Nelson V. Greidanus, and Donald S. Garbuz. "Multivariate analysis of risk factors for re-dislocation after revision for dislocation after total hip arthroplasty." HIP International 30, no. 1 (March 19, 2019): 93–100. http://dx.doi.org/10.1177/1120700019831628.

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Background:The treatment for recurrent dislocation of a total hip arthroplasty is surgical using varied techniques and technologies to reduce the chances of re-dislocation and re-revision. The goal of this study is to compare operative techniques to reduce re-dislocation and re-revision in revision hip arthroplasty due to recurrent dislocations.Methods:A retrospective study of revision hip arthroplasties done due to recurrent dislocation prior to 01 January 2014. Electronic physician and provincial health records were used to collect patients’ initial and follow-up data. Treatment failure was defined as either aseptic re-revision or re-dislocation without revision. Time to event was considered as the re-revision date or the date of second dislocation when the latter endpoint was used.Results:Of 379 operations, 88 (23.2%) had aseptic repeat revision or recurrent dislocation. Of these: 66 (75.0%) due to dislocation with re-revision; 10 (11.4%) due to dislocation with no re-revision surgery; 5 (5.7%) due to aseptic loosening of components; 3 (3.4%) due to osteolysis; 3 (3.4%) due to pseudotumour; and 1 (1.1%) due to periprosthetic fracture. The following factors increase risk of failure: the use of augmented-liners (lipped, oblique and high-offset liners; HR = 1.68, 95% CI, 1.05–2.69), periprosthetic femur fracture (HR = 2.80, 95% CI, 1.39–8.21) and pelvic discontinuity (HR = 3.69, 95% CI, 1.66–8.21). Femur head sizes 36–40 mm are protective (HR = 0.54, 95% CI, 0.31–0.86). In abductor dysfunction the use of focal constrained liners decreases the risk of failure (HR = 0.13, 95% CI, 0.018–0.973).Conclusions:Larger head sizes and focal constrained liners (abductors dysfunction) should be used and fully constrained liners and augmented-liners should be avoided in a revision hip arthroplasty due to recurrent dislocations.

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Gálvez-Sirvent, Elena, Aitor Ibarzábal-Gil, and E. Carlos Rodríguez-Merchán. "Treatment options for aseptic tibial diaphyseal nonunion: A review of selected studies." EFORT Open Reviews 5, no. 11 (November 2020): 835–44. http://dx.doi.org/10.1302/2058-5241.5.190077.

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In aseptic tibial diaphyseal nonunions after failed conservative treatment, the recommended treatment is a reamed intramedullary (IM) nail. Typically, when an aseptic tibial nonunion previously treated with an IM nail is found, it is advisable to change the previous IM nail for a larger diameter reamed and locked IM nail (the rate of success of renailing is around 90%). A second change after an IM nail failure is also a good option, especially if bone healing has progressed after the first change. Fibular osteotomy is not routinely advised; it is only recommended when it interferes with the nonunion site. In delayed unions before 24 weeks, IM nail dynamization can be performed as a less invasive option before deciding on a nail change. If there is a bone defect, a bone graft must be recommended, with the gold standard being the autologous iliac crest bone graft (AICBG). A reamer-irrigator-aspirator (RIA) system might also obtain a bone autograft that is comparable to AICBG. Although the size of the bone defect suitable to perform bone transport techniques is a controversial issue, we believe that such techniques can be considered in bone defects > 3 cm. Non-invasive therapies and biologic therapies could be applied in isolation for patients with high surgical risk, or could be used as adjuvants to the aforementioned surgical treatments. Cite this article: EFORT Open Rev 2020;5:835-844. DOI: 10.1302/2058-5241.5.190077

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O’Sullivan, Denis, Hazel McArdle, Sing Wei Ng, Paula Bourke, Robert Forster, and Liam O’Neill. "Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method." Applied Sciences 10, no. 19 (September 24, 2020): 6670. http://dx.doi.org/10.3390/app10196670.

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Collagen coatings were applied onto polystyrene microplates using a cold atmospheric pressure plasma process. The coatings were compared to standard wet chemical collagen thin films using microscopy, surface energy, infra-red spectroscopy, electrophoresis, and cell culture techniques. Thin films were also deposited on gold electrodes using both coating methods and their structural and barrier properties probed using cyclic voltammetry. While the wet chemical technique produced a thicker deposit, both films appear equivalent in terms of coverage, porosity, structure, and chemistry. Significantly, the cold plasma method preserves both the primary and secondary structure of the protein and this results in high biocompatibility and cell activity that is at least equivalent to the standard wet chemical technique. The significance of these results is discussed in relation to the benefits of a single step plasma coating in comparison to the traditional multi-step aseptic coating technique.

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Gupta, Nikita, Vidhi Jain, Merin Rosy Joseph, and Siwani Devi. "A Review on Micropropagation Culture Method." Asian Journal of Pharmaceutical Research and Development 8, no. 1 (February 14, 2020): 86–93. http://dx.doi.org/10.22270/ajprd.v8i1.653.

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Micropropagation is a vegetative propagation conducted under controlled and aseptic conditions in the microenvironment of the culture vessel, which have the all growth requirements of a plant in the natural conditions. Recently different techniques of propagation have been developed which could facilitate large scale production of plants and for the improvement of the species. An overview on the in vitro propagation via meristem culture, callus culture and protoplast culture etc. are presented here. Today micropropagation techniques are applied in order to produce large numbers of new high-quality plants in a relatively short time and space, in low cost and can also be preserved.

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Britt, Rebecca C. "The Glove Made from Love: A History of Surgical Attire." American Surgeon 85, no. 9 (September 2019): 935–38. http://dx.doi.org/10.1177/000313481908500931.

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The development of surgical attire is well documented in historical photographs and evolved in response to the changing understanding of aseptic and antiseptic techniques. Surgeons throughout time remained significantly opposed to changes in attire, and it was over a century that we evolved from wearing black frock coats to the current attire of today. Interestingly, surgical attire remains a source of controversy even today, with a recent argument regarding skull versus bouffant caps that was quite publicly debated.

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Attin, M. "Electrophysiology study: a comprehensive review." American Journal of Critical Care 10, no. 4 (July 1, 2001): 260–73. http://dx.doi.org/10.4037/ajcc2001.10.4.260.

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An EPS provides valuable information about cardiac arrhythmias by recording intracardiac electrograms and using programmed stimulation techniques. Although the sequence of study protocols may vary with each institution, an EPS routinely begins with baseline measurement of conduction intervals. Subsequently, clinical arrhythmias are induced either by incremental or decremental pacing and extrastimulus techniques. Nurses must understand the technical and psychosocial aspects of an EPS to ensure adequate care. The primary role of nurses is to maintain a safe environment for the patient in the electrophysiology laboratory (i.e., maintain aseptic techniques and intervene when complications occur). Rapid technological advances and the increasing number of well-educated patients require that nurses be knowledgeable and skilled in providing progressive care, such as developing educational programs and support groups for patients and patients' families.

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Schultz, Kathryn Hahne, and Stephen Garton. "Propagation of a Threatened Plant Species: American Hart's-Tongue Fern." HortScience 30, no. 4 (July 1995): 913D—913. http://dx.doi.org/10.21273/hortsci.30.4.913d.

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Phyllitis scolopendrium var. americana is a rare North American fern species whose range is being threatened by habitat alteration. Research efforts were undertaken to ensure survival of the southern population by applying horticultural techniques to propagate new plants for reintroduction. Several techniques were used to induce spore germination. Aseptic techniques included direct plating of spores onto agar medium in petri dishes and dusting spores into test tubes filled with liquid medium. Spores were spread onto soil samples taken from the fern habitats and onto various other propagation media. Prothalli grown in nutrient solution were transferred onto various nonsterile conventional horticultural media. Results indicated that germination in the nutrient solution and subsequent transfer of prothalli was more efficient and promoted survival of propagules compared to subculture from solidified medium.

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Mar, Winnie, Isaiah Tan, Albert Song, Imran Omar, Mihra Taljanovic, and Gregory Stacy. "Update on Imaging of Knee Arthroplasties: Normal Findings and Hardware Complications." Seminars in Musculoskeletal Radiology 23, no. 02 (March 29, 2019): e20-e35. http://dx.doi.org/10.1055/s-0039-1677697.

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AbstractTotal knee arthroplasty (TKA) is the most common joint replacement performed. This article reviews the normal appearance of TKA including the most common types of arthroplasties as well as complications. Common complications at the present time are infection, aseptic loosening, and instability. Rarer complications such as polyethylene wear, periprosthetic fracture, and soft tissue pathology are also discussed. Although the mainstay of imaging is radiographs, newer techniques in TKA imaging such as computed tomography and magnetic resonance imaging are also reviewed.

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Stojakowska, Anna, Janusz Malarz, and Wanda Kisiel. "Thymol Derivatives from a Root Culture of Inula helenium." Zeitschrift für Naturforschung C 59, no. 7-8 (August 1, 2004): 606–8. http://dx.doi.org/10.1515/znc-2004-7-827.

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AbstractA root culture of Inula helenium L. was established from leaf explants of aseptic seedlings. An ethanol extract from the lyophilised roots was fractionated using different chromatographic techniques (CC, TLC). The main secondary metabolites found in the root culture were two thymol derivatives: 10-isobutyryloxy-8,9- epoxy-thymol isobutyrate (1) and 10-isobutyryloxy-6- methoxy-8,9-epoxy-thymol isobutyrate (2). The compounds were identified by spectral methods. Quantification of compound 1 in plant material was done by analytical RP-HPLC.

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Wahrman, Miryam Z., and Corey H. Basch. "Hands-on Research Reaching across Disciplines." American Biology Teacher 81, no. 6 (August 1, 2019): 412–15. http://dx.doi.org/10.1525/abt.2019.81.6.412.

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We describe an interdisciplinary research project for undergraduate students involving microbiology and public health. Students designed and carried out two research studies on hand hygiene and the use of gloves by mobile food vendors in New York City and in a New Jersey mall. Students received training in aseptic techniques and survey methodology to carry out the multifaceted study. We discuss the importance of interdisciplinary collaboration and research in the context of its value in preparing professionals in the fields of biology and public health.

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Corrêa da Silva, Diogo Pedrosa, Elif Aylin Ozudogru, Michele Valquíria Dos Reis, and Maurizio Lambardi. "In vitro conservation of ornamental plants." Ornamental Horticulture 24, no. 1 (March 12, 2018): 28–33. http://dx.doi.org/10.14295/oh.v24i1.1163.

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The market of flowers and ornamental plants is dependent on the diversification of species and the availability of high quality propagation materials. Actually, in vitro culture techniques performance a prominent role in the multiplication and maintenance of commercially propagated ornamental plant species, and are promising for the production of thousands of high quality plants in relatively short term. In addition, when market demand for a particular species is low or zero in a specific period of the year, in vitro culture techniques allow the conservation of cultures under aseptic conditions, by Slow Growth Storage (SGS), from a few weeks to one year (or more), without affecting their viability and potential regrowth. This can be achieved by modifying the constitution of the culture medium and the maintenance conditions of in vitro cultures. Obviously, the success of the technique depends on greatly on the physiological characteristics of the species to be conserved, as well. Once a SGS protocol is optimized, the expenses labor, the possibility of contamination and the probability of somaclonal variation can be reduced markedly.

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Winata, I. Gde Sastra, and Clara Amanda. "Examination and counseling of gynecological cases during Corona Virus Disease 2019 (COVID-19) pandemic." Majalah Obstetri & Ginekologi 29, no. 2 (September 14, 2021): 76. http://dx.doi.org/10.20473/mog.v29i22021.76-83.

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HIGHLIGHTS1. Clinical gynecology examinations must adapt to pandemic situation.2. It must pay attention to universal precautions, aseptic and sterile technique to minimize infection transmission.3. In terms of reducing contact, the need for examination are divided by priority into "now", "soon", or "later".4. Gynecology examinations can be carried out with direct examinations by medical personnel wearing proper personal protective equipment if needed.5. Patient counseling can be done by remote consultation or telemedicine. ABSTRACTCOVID-19 is a recent pandemic caused by the SARS COV-2 agent with a high incidence and mortality. The disease is transmitted through respiratory droplets and direct contact. Clinically this COVID-19 patient is mainly related to the respiratory tract symptoms. The current clinical classifications are divided into suspected, probable, and confirmed cases. To reduce transmission must pay attention to universal and hierarchical precaution, aseptic standards, and sterile techniques. The types of gynecological examinations during a pandemic are the same as those in general, except that the methods, settings, and priorities are different. The examination begins with screening to assess the risk of transmission so that it can determine the place of examination. The urgency of the examination, history of TOCC, local transmission, provider, and room conditions also need to be considered. Counseling during a pandemic can be done in person or by telemedicine. Counseling is provided for general and case-specific gynecological information. Each gynecological case requires a different focus on counseling.

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Pujol, Laure, Isabelle Albert, Nicholas Brian Johnson, and Jeanne-Marie Membré. "Potential application of quantitative microbiological risk assessment techniques to an aseptic-UHT process in the food industry." International Journal of Food Microbiology 162, no. 3 (April 2013): 283–96. http://dx.doi.org/10.1016/j.ijfoodmicro.2013.01.021.

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Journal articles on the topic 'Aseptic techniques'

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