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1

Neal Peterson, R. "PAWPAW (ASIMINA)." Acta Horticulturae, no. 290 (May 1991): 569–602. http://dx.doi.org/10.17660/actahortic.1991.290.13.

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2

Zhao, Geng-Xian, Laura R. Miesbauer, David L. Smith, and Jerry L. McLaughlin. "Asimin, Asiminacin, and Asiminecin: Novel Highly Cytotoxic Asimicin Isomers from Asimina triloba." Journal of Medicinal Chemistry 37, no. 13 (June 1994): 1971–76. http://dx.doi.org/10.1021/jm00039a009.

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3

Majrashi, Taghreed A., Fazila Zulfiqar, Amar G. Chittiboyina, Zulfiqar Ali, and Ikhlas A. Khan. "Isoquinoline alkaloids from Asimina triloba." Natural Product Research 33, no. 19 (November 20, 2018): 2823–29. http://dx.doi.org/10.1080/14786419.2018.1504045.

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4

Rascoe, Danielle, Kirk W. Pomper*, Jeremiah Lowe, Sheri B. Crabtree, Har Mahdeem, and Tejender S. Kochhar. "Assessment of the Utility of ISSR Markers for Evaluating Genetic Relationships Among Members of Asimina and Annona." HortScience 39, no. 4 (July 2004): 786A—786. http://dx.doi.org/10.21273/hortsci.39.4.786a.

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The genus Asimina has the only temperate representatives of the tropical Annonaceae, or Custard Apple family, and includes eight species that are indigenous to North America. The North American pawpaw Asimina triloba (L.) Dunal has the largest edible fruit native to the United States and is the best-known of these species. The USDA National Clonal Germplasm Repository for Asimina species is located at Kentucky State Univ. (KSU); therefore, assessment of genetic diversity is an important research priority for KSU. The inter-simple sequence repeat PCR (ISSR-PCR) methodology has been used successfully to characterize genetic diversity within and among populations of many plant species. The objective of this study was to assess the utility of ISSR markers in evaluating genetic relationships in members of the Asimina genus, as well as closely related tropical relatives in the Annona genus. Leaf samples were collected from three plants each of Asimina longifolia, A. obovata, A. parviflora, A. reticulata, A. tetramera and A. triloba. Leaf samples were also collected from three plants each of Annona cherimola, A. squamosa, A. reticulata, A. muricata, A. glabra, A. diversifolia, and A. montana. DNA was extracted from leaf samples and subjected to ISSR-PCR using the REDExtract-N-Amp™ Plant PCR Kit. DNA samples were screened with ISSR primers using the Univ. of British Columbia microsatellite primer set #9. Three primers, UBC812, UBC841, and UBC873 were found to produce 84 scorable ISSR markers and allowed the determination of genetic relationships among Asimina and Annona members examined.
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5

Norman, Eliane M., Kathleen Rice, and Steven Cochran. "Reproductive Biology of Asimina parviflora (Annonaceae)." Bulletin of the Torrey Botanical Club 119, no. 1 (January 1992): 1. http://dx.doi.org/10.2307/2996913.

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6

Wood, Randal, and Scott Peterson. "Lipids of the pawpaw fruit: Asimina triloba." Lipids 34, no. 10 (October 1999): 1099–106. http://dx.doi.org/10.1007/s11745-999-0461-x.

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7

A.O., Saldetska, Pavlov V.V, and Pavlova N.R. "СHARACTERISTIC OF THE ANATOMICAL STRUCTURE OF THE ASIMINA TRILOBA L." Scientific Bulletin of Natural Sciences (Biological Sciences), no. 26 (August 23, 2019): 122–31. http://dx.doi.org/10.32999/ksu2524-0838/2019-26-12.

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8

ZHAO, G. X., L. R. MIESBAUER, D. L. SMITH, and J. L. MCLAUGHLIN. "ChemInform Abstract: Asimin (Ib), Asiminacin (Ic), and Asiminecin (Id): Novel Highly Cytotoxic Asimicin (Ia) Isomers from Asimina triloba." ChemInform 25, no. 50 (August 18, 2010): no. http://dx.doi.org/10.1002/chin.199450268.

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9

Huang, Hongwen, Desmond R. Layne, and Thomas L. Kubisiak. "RAPD Inheritance and Diversity in Pawpaw (Asimina triloba)." Journal of the American Society for Horticultural Science 125, no. 4 (July 2000): 454–59. http://dx.doi.org/10.21273/jashs.125.4.454.

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Twelve, 10-base primers amplified a total of 20 intense and easily scorable polymorphic bands in an interspecific cross of PPF1-5 pawpaw [Asimina triloba (L.) Dunal.] × RET (Asimina reticulata Shuttlew.). In this cross, all bands scored were present in, and inherited from, the A. triloba parent PPF1-5. Nineteen of the 20 bands were found to segregate as expected (1:1 or 3:1) based on chi-square goodness-of-fit tests, and were subsequently used to evaluate genetic diversity in populations of A. triloba collected from six states (Georgia, Illinois, Indiana, Maryland, New York, and West Virginia) within its natural range. Analysis of genetic diversity of the populations revealed that the mean number of alleles per locus was A = 1.64, percent polymorphic loci was P = 64, and expected heterozygosity was He = 0.25. No significant differences were found among populations for any of the polymorphic indices. Partitioning of the population genetic diversity showed that the average genetic diversity within populations was Hs = 0.26, accounting for 72% of the total genetic diversity. Genetic diversity among populations was Dst = 0.10, accounting for 28% of the total genetic diversity. Nei's genetic identity and distance showed a high mean identity of 0.86 between populations. Genetic relationships among the populations examined by unweighted pair-group mean clustering analysis separated the six populations into two primary clusters: one composed of Georgia, Maryland, and New York, and the other composed of Illinois, Indiana, and West Virginia. The Georgia and Indiana populations were further separated from the other populations within each group. This study provides additional evidence that marginal populations within the natural range of A. triloba should be included in future collection efforts to capture most of the rare and local alleles responsible for this differentiation.
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10

Pomper, Kirk W., Jeremiah D. Lowe, Li Lu, Sheri B. Crabtree, and Lauren A. Collins. "Clonality of Pawpaw (Asimina triloba) Patches in Kentucky." Journal of the Kentucky Academy of Science 70, no. 1 (March 2009): 3–11. http://dx.doi.org/10.3101/1098-7096-70.1.3.

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11

Stănică, F., R. Cotruţ, and G. Zuccherelli. "NEW SELECTIONS OF PAWPAW (ASIMINA TRILOBA (L) DUNAL)." Acta Horticulturae, no. 772 (August 2008): 203. http://dx.doi.org/10.17660/actahortic.2008.772.28.

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12

Potts, Lisa F., Frederick A. Luzzio, Scott C. Smith, Michal Hetman, Pierre Champy, and Irene Litvan. "Annonacin in Asimina triloba fruit: Implication for neurotoxicity." NeuroToxicology 33, no. 1 (January 2012): 53–58. http://dx.doi.org/10.1016/j.neuro.2011.10.009.

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13

He, Kan, Geng-Xian Zhao, Guoen Shi, Lu Zeng, Jin-Feng Chao, and Jerry L. McLaughlin. "Additional bioactive annonaceous acetogenins from Asimina triloba (Annonaceae)." Bioorganic & Medicinal Chemistry 5, no. 3 (March 1997): 501–6. http://dx.doi.org/10.1016/s0968-0896(96)00264-7.

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14

Hawkins, Robert. "Propagation protocol for pawpaw (Asimina triloba (L.) Dunal)." Native Plants Journal 19, no. 3 (2018): 248–53. http://dx.doi.org/10.3368/npj.19.3.248.

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15

Shiota, Haruyasu. "Volatile components of pawpaw fruit (Asimina triloba Dunal)." Journal of Agricultural and Food Chemistry 39, no. 9 (September 1991): 1631–35. http://dx.doi.org/10.1021/jf00009a019.

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16

Ratnayake, Sunil, Xin-ping Fang, Jon E. Anderson, Jerry L. McLaughlin, and Dean R. Evert. "Bioactive Constituents from the Twigs of Asimina parviflora." Journal of Natural Products 55, no. 10 (October 1992): 1462–67. http://dx.doi.org/10.1021/np50088a012.

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17

Damman, Hans. "Facilitative interactions between two lepidopteran herbivores of Asimina." Oecologia 78, no. 2 (February 1989): 214–19. http://dx.doi.org/10.1007/bf00377158.

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18

Sica, V. P., T. El-Elimat, and N. H. Oberlies. "In situ analysis of Asimina triloba (paw paw) plant tissues for acetogenins via the droplet-liquid microjunction-surface sampling probe coupled to UHPLC-PDA-HRMS/MS." Analytical Methods 8, no. 32 (2016): 6143–49. http://dx.doi.org/10.1039/c6ay01583b.

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19

Baumer, Marilyn, and James R. Runkle. "Tree Seedling Establishment Under the Native Shrub, Asimina triloba." Castanea 75, no. 4 (December 2010): 421–32. http://dx.doi.org/10.2179/09-024.1.

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20

Ayala-Silv, Tomas, Caula A. Beyl, and Greg Dortch. "Agrobacterium rhizogenes Mediated-transformation of Asimina triloba L. Cuttings." Pakistan Journal of Biological Sciences 10, no. 1 (December 15, 2006): 132–36. http://dx.doi.org/10.3923/pjbs.2007.132.136.

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21

Haribal, Meena, Paul Feeny, and Cathy C. Lester. "A Caffeoylcyclohexane-1-Carboxylic Acid Derivative From Asimina Triloba." Phytochemistry 49, no. 1 (September 1998): 103–8. http://dx.doi.org/10.1016/s0031-9422(97)00804-2.

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22

Zhao, G. X., M. J. Rieser, Y. H. Hui, L. R. Miesbauer, D. L. Smith, and J. L. McLaughlin. "Biologically active acetogenins from stem bark of Asimina triloba." Phytochemistry 33, no. 5 (July 1993): 1065–73. http://dx.doi.org/10.1016/0031-9422(93)85024-l.

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23

Huang, Hongwen, Desmond R. Layne, and Thomas L. Kubisiak. "Molecular Characterization of Cultivated Pawpaw (Asimina triloba) Using RAPD Markers." Journal of the American Society for Horticultural Science 128, no. 1 (January 2003): 85–93. http://dx.doi.org/10.21273/jashs.128.1.0085.

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Thirty-four extant pawpaw [Asimina triloba (L.) Dunal] cultivars and advanced selections representing a large portion of the gene pool of cultivated pawpaws were investigated using 71 randomly amplified polymorphic DNA (RAPD) markers to establish genetic identities and evaluate genetic relatedness. All 34 cultivated pawpaws were uniquely identified by as few as 14 loci of eight primers. Genetic diversity of the existing gene pool of cultivated pawpaws, as estimated by Nei's gene diversity (He), was similar to that of wild pawpaw populations. The genetic relatedness among the cultivated pawpaws examined by UPGMA cluster analysis separated 34 cultivars and selections into two distinct clusters, a cluster of PPF (The PawPaw Foundation) selections and a cluster including a majority of the extant cultivars selected from the wild and their derived selections. The results are in general agreement with the known selection history and pedigree information available. The consensus fingerprint profile using the genetically defined RAPD markers is a useful and reliable method for establishing the genetic identities of the pawpaw cultivars and advanced selections. This also proved to be an improved discriminating tool over isozyme markers for the assessment of genetic diversity and relatedness. RAPD profiling of data presented in this study provides a useful reference for germplasm curators engaged in making decisions of sampling strategies, germplasm management and for breeders deciding which parents to select for future breeding efforts.
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24

Geneve, R. L., S. T. Kester, and K. W. Pomper. "CYTOKININ HABITUATION FOR AUTONOMOUS SHOOT INITIATION IN PAWPAW (ASIMINA TRILOBA)." Acta Horticulturae, no. 738 (March 2007): 371–74. http://dx.doi.org/10.17660/actahortic.2007.738.44.

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25

Standley, Lisa A., and Barbara Katzenberg. "PAWPAW ASIMINA TRILOBA (ANNONACEAE): A RECORD OF ESTABLISHMENT IN MASSACHUSETTS." Rhodora 121, no. 985 (May 3, 2019): 54. http://dx.doi.org/10.3119/18-10.

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26

Huang, Hongwen, Desmond R. Layne, and Thomas L. Kubisiak. "Pawpaw [Asimina triloba (L.) Dunal] Germplasm Evaluation Using RAPD Markers." HortScience 32, no. 3 (June 1997): 513D—513. http://dx.doi.org/10.21273/hortsci.32.3.513d.

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Kentucky State Univ. (KSU) is the national clonal germplasm repository for Asimina species. Previous evaluation of the KSU pawpaw collection using 24 isozyme markers demonstrated that pawpaw has a relatively higher genetic diversity than that noted for other plant species with similar species characteristics (long-lived, woody, perennial, out-crossing, temperate, widespread, etc.). Current evaluation using RAPD markers will provide us with a more-accurate insight into pawpaw genetic diversity and population structure. In a preliminary experiment, one hundred 10-mer primers (OA1-20 through OE1-20, Operon Technologies Inc.) were screened against 32 commercial cultivars or advanced selections. A subset of 24 primers that amplify only the most-informative markers were used for germplasm evaluation. Sixty-eight RAPD markers were identified and used for determining genetic parameters. One-hundred-twenty pawpaw accessions were sampled from the KSU repository for RAPD analysis. These accessions represented nine widely distributed states within pawpaw's native range. RAPD data were subjected to various analyses using the NTSYS-PC computer program (ver. 1.8). Information generated from isozyme and RAPD markers will be used to formulate future germplasm collection strategies from wild populations within the native range. The implications of such information to the genetic enhancement of our repository and establishment of a core collection will be discussed.
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27

Ye, Quin, Lu Zeng, Yan Zhang, Geng-Xian Zhao, Jerry L. McLaughlin, Mi Hee Woo, and Dean R. Evert. "Longicin and Goniothalamicinone: Novel Bioactive Monotetrahydrofuran Acetogenins from Asimina longifolia." Journal of Natural Products 58, no. 9 (September 1995): 1398–406. http://dx.doi.org/10.1021/np50123a010.

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28

Кrasovsky, Volodymyr. "Формування інтродукційних популяцій субтропічних плодових культур у Хорольському ботанічному саду." Lesya Ukrainka Eastern European National University Scientific Bulletin. Series: Biological Sciences, no. 7(356) (June 26, 2018): 7–12. http://dx.doi.org/10.29038/2617-4723-2017-356-7-7-12.

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Зазначено, що формування інтродукційних популяцій Asimina triloba (L.) Dunal, Punica granatum L., Zizyphus jujuba Mill., Ficus carica L., Amygdalus communis L., Diospyros virginiana L. у лісостеповій зоні України дає змогу створити біотичні системи субтропічних плодових культур, які характеризуються певною екологічною й генетичною структурою цих видів, де реалізуються гібридизаційні процеси.
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29

Crabtree*, Sheri B., Kirk W. Pomper, and Robert L. Geneve. "Trench Layering as a Method of Clonally Propagating Pawpaw (Asimina triloba)." HortScience 39, no. 4 (July 2004): 890B—890. http://dx.doi.org/10.21273/hortsci.39.4.890b.

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The pawpaw (Asimina triloba) is the largest fruit native to the U.S. and has potential as a new fruit crop. Few methods are available to clonally propagate pawpaw, with grafting or budding onto a seedling rootstock being the only currently feasible method. Developing new options for clonal propagation of pawpaw could help advance this growing industry. Layering has been used to clonally propagate other difficult to root tree species. The objective of this study was to examine trench layering as a method to clonally propagate pawpaw. A randomized factorial experiment was implemented to examine the roles of plant juvenility and auxin concentration on rooting in a greenhouse trench layering system. Seedlings were defoliated, tipped, and transplanted into trench layering beds at 3, 6, and 12 weeks after emergence. Shoots were etiolated, then girdled and treated with three levels of IBA (0, 5000, and 10,000 ppm). The main effects of age and IBA concentration significantly affected the percentage of shoots producing roots. Juvenility enhanced rooting, with 15% of the shoots of the 3-week-old pawpaw seedlings producing roots, compared to only about 5% of the 12-week-old seedlings rooting. Auxin application to shoots also promoted rooting, with 16% of IBA-treated shoots producing roots, compared to the untreated control, with only 2% of shoots producing roots. There was no significant difference in rooting percentage between the two concentrations of IBA. The treatment combination most successful at promoting root initiation was 10,000 ppm IBA applied to shoots of 3-week-old seedlings, with 31% of shoots rooting.
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30

Huang, Hongwen, and Desmond R. Layne. "Allozyme Variation and Geographic Differentiation in Pawpaw [Asimina triloba (L.) Dunal]." HortScience 31, no. 4 (August 1996): 592a—592. http://dx.doi.org/10.21273/hortsci.31.4.592a.

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The pawpaw is the largest tree fruit native to the United States and the only temperate member of the tropical Custard Apple family (Annonaceae). In 1995, Kentucky State Univ. was established as the USDA-ARS-National Clonal Germplasm Repository for Asimina spp. Seedling trees from 400 pawpaw accessions representing 70 distinct geographic regions from 17 states are currently being grown at our research farm. In a preliminary study, 18 pawpaw cultivars were assayed in 30 enzyme systems using an isoelectric focusing polyacrylamide slab gel system of pH 4-9. Twelve enzymes produced high resolution without tissue specificity and were further used for evaluation of allozyme diversity of geographic populations. Degree of genetic diversity within populations and differentiation between populations as evaluated by the expected heterozygosity (He), the proportion of polymorphic loci (P), the average number of alleles per locus (A), chi-squared analysis of allele frequency heterogeneity, Nei's standard genetic distance (D), and identity (I) will be discussed. Dendrograms were generated by cluster analysis using the unweighted pair group method to demonstrate the relationships of geographic populations in the 17 states evaluated. The strategy for germplasm conservation and cultivar development through breeding will also be discussed.
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31

L. McLaughlin, Jerry, Geng-xian Zhao, Jocelyn H. Ng, John F. Kozlowzki, and David L. Smith. "Bullatin and Bullanin: Two Novel, Highly Cytotoxic Acetogenins from Asimina triloba." HETEROCYCLES 38, no. 8 (1994): 1897. http://dx.doi.org/10.3987/com-94-6765.

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32

Goodrich, Katherine R., and Robert A. Raguso. "The olfactory component of floral display in Asimina and Deeringothamnus (Annonaceae)." New Phytologist 183, no. 2 (May 21, 2009): 457–69. http://dx.doi.org/10.1111/j.1469-8137.2009.02868.x.

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33

FANG, CAODI, CHANGZHENG WANG, YOULING L. XIONG, and KIRK W. POMPER. "EXTRACTION AND CHARACTERIZATION OF POLYPHENOL OXIDASE IN PAWPAW (ASIMINA TRILOBA) FRUIT." Journal of Food Biochemistry 31, no. 5 (October 2007): 603–20. http://dx.doi.org/10.1111/j.1745-4514.2007.00133.x.

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34

Кrasovsky, Volodymyr, and Тaisiya Chernyak. "Ризики культивування субтропічних плодових культур у лісостеповій зоні України." Lesya Ukrainka Eastern European National University Scientific Bulletin. Series: Biological Sciences, no. 12(337) (January 23, 2019): 4–11. http://dx.doi.org/10.29038/2617-4723-2016-337-12-4-11.

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На прикладі Полтавської обл., природно-кліматичні умови якої є типовими для фізико-географічної зони Лісостепу України, показано, що в окремі роки в зимовий період сукупна дія низьких від’ємних температур повітря та вітру може бути критичною з огляду на морозостійкість інтродукованих видів Asimina triloba (L.) Dunal, Punica granatum L., Zizyphus jujuba Mill., Ficus carica L., Amygdalus communis L., Diospyros virginiana L.
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35

Koslanund, Rumphan, Douglas D. Archbold, and Kirk W. Pomper. "Pawpaw [Asimina triloba (L.) Dunal] Fruit Ripening. I. Ethylene Biosynthesis and Production." Journal of the American Society for Horticultural Science 130, no. 4 (July 2005): 638–42. http://dx.doi.org/10.21273/jashs.130.4.638.

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Pawpaw fruit ethylene production, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) and ACC oxidase (ACO) activities, and tissue content of the ethylene precursor ACC and conjugate malonyl-ACC (MACC) were measured during postharvest ripening. Fruit were harvested near the advent of the ripening process and were ripened at room temperature. The fruit displayed increases in ethylene production and respiration rate during ripening with maxima for both 3 days after harvest. Mean ethylene maxima on a fresh weight basis were 4.7 and 7.6 μg·kg-1·h-1 and mean respiratory (CO2 production) maxima on a fresh weight basis were 220 and 239 mg·kg-1·h-1 in 1999 and 2001, respectively. The increase in ethylene evolution coincided with an increase in respiration and a rapid decline in fruit firmness. Internal and external fruit firmness declined in a parallel manner. The ethylene climacteric peak occurred after the greatest decline in fruit firmness, indicating that low levels of ethylene may be sufficient to initiate the ripening process. The ethylene climacteric peak also coincided with the highest activities of both ACS and ACO as well as the maximum tissue ACC content. As ACC content increased, MACC content declined, suggesting a regulation of ethylene production via free ACC levels by malonylation of ACC. Thus, the climacteric development of ethylene production may be regulated by an increase of ACS activity and a decrease in ACC malonyltransferase activity, making more free ACC available for the production of ethylene by increased activity of ACO.
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36

Norman, Eliane M., and David Clayton. "Reproductive Biology of two Florida Pawpaws: Asimina obovata and A. pygmaea (Annonaceae)." Bulletin of the Torrey Botanical Club 113, no. 1 (January 1986): 16. http://dx.doi.org/10.2307/2996229.

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37

Callaway, M. Brett. "Current Research for the Commercial Development of Pawpaw [Asimina triloba (L.) Dunal]." HortScience 27, no. 2 (February 1992): 90–191. http://dx.doi.org/10.21273/hortsci.27.2.90.

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38

Levine, Robert A., Kristy M. Richards, Kevin Tran, Rensheng Luo, Andrew L. Thomas, and Robert E. Smith. "Determination of Neurotoxic Acetogenins in Pawpaw (Asimina triloba) Fruit by LC-HRMS." Journal of Agricultural and Food Chemistry 63, no. 4 (January 22, 2015): 1053–56. http://dx.doi.org/10.1021/jf504500g.

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39

Olson, Matthew G., and Michael T. Keeley. "Controlling Dense Layers of Pawpaw (Asimina triloba) in Mesic Upland Forest Understories." Forest Science 64, no. 3 (March 27, 2018): 324–29. http://dx.doi.org/10.1093/forsci/fxx015.

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40

TABACU, Andrei Florin, Ana Cornelia BUTCARU, Andreea STAN, Cosmin Alexandru MIHAI, and Florin STĂNICĂ. "Pawpaw Hybrid Genotypes (Asimina triloba (L.) Dunal) Cultivated in the Bucharest Area." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture 77, no. 2 (November 18, 2020): 103. http://dx.doi.org/10.15835/buasvmcn-hort:2020.0014.

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Pawpaw or Northern banana (Asimina triloba (L.) Dunal) is the only representative of the Annonaceae family that can be cultivated in temperate areas. The increasing demands from the consumers for this nearly unknown fruit with high nutraceutical potential require specific researches. The aim of this study is to present the fruits pomological characteristics for 23 genotypes analyzed, cultivated in the Bucharest area. For each genotype yield, fruit size, average number of seeds per fruit, average weight of seeds and seeds size were measured. Fruit biochemical analyses as dry matter, total soluble solids, ascorbic acid content, acidity, were done for several genotypes. Fruit quality evaluation through sensorial analyses was conducted for 13 genotypes. The most valuable genotypes from the Experimental field according to the analyses performed and the consumer’s preferences are taken in consideration for further research.
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41

Lolletti, D., L. Principio, R. Ciorba, F. Mitrano, D. Ceccarelli, F. Antonucci, R. Manganiello, and R. Ciccoritti. "Asimina triloba: Crop years, cultivars and ripening time influence on qualitative parameters." Scientia Horticulturae 289 (November 2021): 110481. http://dx.doi.org/10.1016/j.scienta.2021.110481.

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42

Ratnayake, Sunil, Zhe-Ming Gu, Laura R. Miesbauer, David L. Smith, Karl V. Wood, Dean R. Evert, and Jerry L. McLaughlin. "Parvifloracin and parviflorin: cytotoxic bistetrahydrofuran acetogenins with 35 carbons from Asimina parviflora (Annonaceae)." Canadian Journal of Chemistry 72, no. 2 (February 1, 1994): 287–93. http://dx.doi.org/10.1139/v94-044.

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Using lethality to brine shrimp, activity-directed fractionation of extracts of Asimina parviflora (Michx.) Dunal. (Annonaceae) has led to the isolation of two new and four known acetogenins (1–6). Compound 1, parvifloracin, is the first nonadjacent bistetrahydrofuran acetogenin with 35 carbons. Compound 2, parviflorin, is an unusual adjacent bistetrahydrofuran acetogenin with 35 carbons. Bullatacin (3), molvizarin (4), annonacin (5), and goniothalamicin (6) are also reported for the first time from this plant. Brine shrimp lethality test data and comparative cytotoxicities of 1–6 against three human solid tumor cell lines are presented.
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43

L. McLaughlin, Jerry, J. Kent Rupprecht, Ching-jer Chang, John M. Cassady, Kenneth L. Mikolkajczak, and David Weisleder. "Asimicin, a New Cytotoxic and Pesticidal Acetogenin from the Pawpaw, Asimina triloba (Annonaceae)." HETEROCYCLES 24, no. 5 (1986): 1197. http://dx.doi.org/10.3987/r-1986-05-1197.

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44

Brannan, Robert G. "Polyphenol Oxidase in Pawpaw (Asimina triloba [L.] Dunal) Fruit Pulp from Different Varieties." Journal of Food Research 5, no. 1 (December 22, 2015): 33. http://dx.doi.org/10.5539/jfr.v5n1p33.

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Pawpaw (<em>Asimina triloba</em> L. Dunal) is a tree fruit from the tropical Annonaceae family. Pawpaw currently has very limited commercial production because of its high perishability. Polyphenol oxidase (PPO) is responsible for enzymatic browning in pawpaw pulp. The objective of this research is to characterize PPO extracted from different pawpaw varieties. With respect to PPO activity, six of the varieties (Taytwo, Rebecca’s Gold, NC-1, Overleese, Rappahannock, and Green River Belle) exhibited PPO activity that was statistically higher than Quaker’s Delight and Lynn’s Favorite. The other four varieties (SAA Zimmerman, Shenendoah, KSU-Atwood, IXL) exhibited PPO activity that was not significantly different from each other or Quaker’s Delight and Lynn’s Favorite, but were significantly lower than Taytwo, Rebecca’s Gold, and NC-1. Kinetic parameters (Vmax, Km) and their ratio can be used to relate enzyme velocity with substrate affinity. Varieties that exhibited a high ratio, i.e. a very active enzyme due to a high Vmax and/or a low Km, are Rebecca’s Gold, Taytwo, NC-1, and KSU-Atwood). The results presented indicate that certain varieties exhibit conditions that suggest PPO could have a lower inherent impact on tissue browning, especially Lynn’s Favorite, Green River Belle, IXL, SAA Zimmerman, and Overleese. On the other hand, certain varieties (Taytwo, Rebecca’s Gold, NC-1, and perhaps KSU Atwood) exhibit PPO activity, Vmax, and Km values that suggest inherently high PPO activity and thus increased potential for browning. Overall, understanding PPO activity may help to explain post-harvest discoloration of pawpaw pulp and aid the commercial selection of more shelf-stable varieties.
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45

Hosaka, Naomi, Sara Gómez, Naoki Kachi, Josef F. Stuefer, and Dennis F. Whigham. "The Ecological Significance of Clonal Growth in the Understory Tree, Pawpaw (Asimina triloba)." Northeastern Naturalist 12, no. 1 (March 2005): 11–22. http://dx.doi.org/10.1656/1092-6194(2005)012[0011:tesocg]2.0.co;2.

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46

Young, Donald R., and Joseph B. Yavitt. "DIFFERENCES IN LEAF STRUCTURE, CHLOROPHYLL, AND NUTRIENTS FOR THE UNDERSTORY TREE ASIMINA TRILOBA." American Journal of Botany 74, no. 10 (October 1987): 1487–91. http://dx.doi.org/10.1002/j.1537-2197.1987.tb12140.x.

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47

Brannan, Robert G., Trisha Peters, and Stephen T. Talcott. "Phytochemical analysis of ten varieties of pawpaw (Asimina triloba [L.] Dunal) fruit pulp." Food Chemistry 168 (February 2015): 656–61. http://dx.doi.org/10.1016/j.foodchem.2014.07.018.

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48

Avalos, Javier, J. Kent Rupprecht, Jerry L. McLaughlin, and Eloy Rodriguez. "Guinea pig maximization test of the bark extract from pawpaw,Asimina triloba(Annonaceae)." Contact Dermatitis 29, no. 1 (July 1993): 33–35. http://dx.doi.org/10.1111/j.1600-0536.1993.tb04533.x.

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49

Brannan, Robert G., and Maria N. Coyle. "Worldwide Introduction of North American Pawpaw (Asimina triloba): Evidence Based on Scientific Reports." Sustainable Agriculture Research 10, no. 3 (May 5, 2021): 19. http://dx.doi.org/10.5539/sar.v10n3p19.

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The North American pawpaw (Asimina triloba) is a small tree native to the Appalachian region of the United States that produces large, yellowish-green to brown fruit that has the flavor of a banana and mango. The scope of this review is limited to pawpaw orchards outside of North America that were intentionally planted for commercial or research purposes and are documented in the scientific literature. This review identified nine countries outside of North America (Romania, Italy, Ukraine, Slovakia, Austria, Georgia, Russia, Japan, Korea) and across two continents that have scientific literature that describes pawpaw cultivation. There are reports that pawpaws are cultivated in China, Israel, Belgium, and Portugal, however, no scientific literature could be found that describes pawpaw research from these countries. Most of the research since 2010 has featured pawpaws grown in Romania, Italy, and Korea with a focus on characterizing roots, twigs, leaves, pulp and seeds or the toxicity and/or bioactivity of the annonaceous acetogenins naturally found in these parts of the pawpaw.
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50

Pomper, Kirk W., Sheri B. Crabtree, Shawn P. Brown, Snake C. Jones, Tera M. Bonney, and Desmond R. Layne. "Assessment of Genetic Diversity of Pawpaw (Asimina triloba) Cultivars with Intersimple Sequence Repeat Markers." Journal of the American Society for Horticultural Science 128, no. 4 (July 2003): 521–25. http://dx.doi.org/10.21273/jashs.128.4.0521.

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The pawpaw [Asimina triloba (L.) Dunal.] is a tree fruit native to many areas of the southeastern and mid-western United States. Kentucky State University (KSU) is designated as a satellite repository for Asimina for the U.S. Department of Agriculture (USDA), National Plant Germplasm System (NPGS). An assessment of the level of genetic diversity in cultivated pawpaw would assist in development of the future germplasm repository collection strategies for cultivar improvement. The objectives of this study were to identify intersimple sequence repeat (ISSR) markers that segregate in a simple Mendelian fashion and to use these markers to assess genetic diversity in 19 pawpaw cultivars. Leaf samples from the 34 progeny of controlled crosses (1-7-1 × 2-54 and reciprocal) and the parents were collected, DNA was extracted, and subjected to the ISSR methodology using the University of British Columbia microsatellite primer set #9. Seven primers yielded 11 Mendelian markers with either a 3:1 or 1:1 ratio that was confirmed by chi-square analysis. Analysis of genetic diversity using 10 of the ISSR markers from 19 pawpaw cultivars revealed a moderate to high level of genetic diversity, with a percent polymorphic loci P = 80 and an expected heterozygosity He = 0.358. These diversity values are higher than those reported for cultivated pawpaw using isozyme or randomly amplified polymorphic DNA (RAPD) markers, indicating that the ISSR marker methodolgy has a higher level of discrimination in evaluating genetic diversity in pawpaw and/or pawpaw has greater levels of genetic diversity than previously found.
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