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1

Lindberg, Beatrice. "Prevalens av ADHD bland fängelsedömda män och kvinnor : Vilka skillnader och likheter finns mellan könen?" Thesis, Högskolan Kristianstad, Sektionen för lärande och miljö, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:hkr:diva-8804.

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Studiens syfte är att undersöka hur stor del av de män och kvinnor som döms till fängelse som har uppmärksamhetsstörning (Attention Deficit Hyperactivity Disorder, ADHD). Den skall även besvara frågan om huruvida det finns skillnader mellan könen i prevalens, samt om det finns korrelation mellan ADHD och Antisocial personlighetsstörning (ASPD). Metoden som används är självskattningsformulär som är välbeprövade inom detta område, Wender-Utah rating Scale (WURS) och Mini Neuropsykiatrisk Intervju (M.I.N.I.). Formulären besvarades av 49 fängelsedömda män och kvinnor, vilka var inskrivna på kriminalvårdsanstalter i mellansverige. Resultatet av studien visar att en hög andel av den aktuella populationen har både ADHD och ASPD. Skillnaderna mellan könen är statistiskt inte signifikanta, men rent procentuellt ligger kvinnor högre i prevalens av ADHD än männen. Resultatet är dock svårtolkat då det var för få manliga deltagare, och bör därmed tolkas försiktigt.
The aim of this study is to investigate how many of the men and women sentenced to prison that suffer from Attention Deficit Hyperactivity Disorder (ADHD). It also aims to answer the question of however there is a difference between sexes according to prevalence, and the correlation between ADHD and antisocial personality disorder (ASPD). The method used is self-report questionnaires which are well tested for this purpose, Wender-Utah Rating Scale (WURS) and Mini Neuropsychiatric Interview (M.I.N.I). The questionnaires were answered by 49 male and female prison inmates, who were residing in prisons in mid-Sweden. The results of the study show that a high number of the population suffers from both ADHD and ASPD. The differences in sexes were not statistically significant, but based on numbers the females are higher in prevalence than the males in ADHD. Since there were few males in this study, the results, however, need to be interpreted with caution.
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2

Sorg, Abberley E. "The Impact of Medicalization on Individuals Labeled with Antisocial Personality Disorder." University of Toledo / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1564670634916647.

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3

Ng, Carol Ka Lo. "Role of aspartate aminotransferases AspB and AspC in the WhiB7-controlled intrinsic drug resistance system of mycobacteria." Thesis, University of British Columbia, 2012. http://hdl.handle.net/2429/43552.

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Intrinsic resistance of the intracellular pathogen Mycobacterium tuberculosis is one of the main reasons that the disease tuberculosis is difficult to treat and why it remains as one of the world’s most prevalent and dangerous infectious diseases. The intrinsic resistance regulator WhiB7 controls a regulon that contains many genes predicted to have physiological functions including aspartate aminotransferases, aspB and aspC. Multi-drug susceptibility was observed in an aspC mutant and an aspB constitutive expression strain. The expression of aspC was positively regulated by WhiB7 while expression of aspB downregulated whiB7 expression. The fitness of Mycobacterium smegmatis was affected negatively by oxaloacetate and positively by α-ketoglutarate, substrates of aspartate aminotransferase, which then altered the growth inhibition by antibiotics. Recombinant AspB and AspC both catalyze measurable transamination of aspartate and α-ketoglutarate. AspC plays an important role in mycobacteria physiology as deletion of this gene caused many growth deficiencies. Furthermore, the physiological role of AspC extended beyond amino acid intermediary metabolism to redox homeostasis and oxidative stress detoxification. These results revealed a link between intrinsic antibiotic resistance and metabolism mediated through AspB and AspC. Since antibiotic resistance in mycobacterium is a complex function of its physiology, it is important to screen for tuberculosis drugs under growth conditions that resemble those found in vivo.
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4

Wellausen, Rafael Stella. "Avaliação dos fatores associados ao uso de álcool e drogas na criminalidade : um estudo no sistema penitenciário." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2009. http://hdl.handle.net/10183/17846.

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A presente Dissertação de Mestrado consiste de um capítulo introdutório na qual são apresentados os quatro eixos que formam a estrutura teórica de dois estudos empíricos apresentados em forma de artigos. Os estudos foram realizados no Presídio Central de Porto Alegre e contaram com uma amostra de 50 prisioneiros sem condenação prévia com idades entre 18 e 35 anos. No primeiro estudo foram utilizados dois instrumentos: O Parental Bonding Instrument (PBI) e a Mini International Neuropsychiatric Interview (MINI). Buscou-se verificar a qualidade dos vínculos afetivos entre os presidiários e seus pais, assim como avaliar os principais quadros psiquiátricos que acometem esta população. Para análise dos dados foram realizadas análises estatísticas com o Teste t e o Teste Qui-quadrado. Os resultados desse estudo revelaram a presença de diversos transtornos psiquiátricos e problemas no vínculos afetivos entre pais e filhos. Houve alta incidência de uso/dependência de drogas sendo a maconha e o crack as mais consumidas. Aqueles prisioneiros que apresentavam um Transtorno Antissocial de Personalidade (TASP) também apresentavam fracasso biparental. Independentemente do diagnóstico psiquiátrico os crimes mais freqüentes foram os contra o patrimônio. O segundo estudo procurou investigar a relação entre uso de álcool e drogas e comportamento sexual de risco para HIV/AIDS. Neste estudo o Addiction Severity Index (ASI6) foi o instrumento utilizado para avaliar gravidade de dependência e comportamento sexual. Os resultados indicaram que com o advento do crack houve uma redução no número de usuários de drogas injetáveis (UDI). Apesar disso, foi verificado que o risco para a contaminação com o vírus do HIV ainda é alto entre os prisioneiros. Duas são as possíveis razoes: um descuido geral em relação à saúde, e o sexo ter-se tornado uma moeda de troca por drogas. Ambos os estudos têm relevância uma vez que revelaram aspectos pouco conhecidos sobre a realidade daqueles que se encontram aprisionados.
The present Master's Dissertation consists of an introductory chapter in which four axes are presented. They constitute the theoretical structure involving two empirical studies presented in article format. The studies were carried out at the Presídio Central in Porto Alegre. The sample comprised 50 inmates, ranging from 18 to 35 years old. In the first study, two instruments were used: The Parental Bonding Instrument (PBI) and the Mini International Neuropsychiatric Interview (MINI). It attempted to verify the quality of affective bonds between the inmates and their parents as well as to assess the main psychiatric histories which affected this population. For the data statistical analysis, the t Test and the Chi-square Test were used. The results revealed the presence of several psychiatric disorders and problems involving the affective bonds between parents and their sons. There was a high incidence of the use/dependency of drugs, with marijuana and crack being the most used. Those inmates who presented Antisocial Personality Disorder (ASPD) also presented biparental failure. Independently from the psychiatric diagnosis, the most frequent crimes are the ones against the patrimony. The second study investigated the relation between the use of drugs and the risky sexual behavior for HIV/AIDS. In this study, the Addiction Severity index (ASI6) was the instrument used to assess the severity of dependency and sexual behavior. The results showed that, with the advent of crack, there was a decrease in the number of injecting drug users (IDU). Nevertheless, it was found that the risk for contamination with the HIV virus is still high among the inmates. There are two possible reasons for this to happen: a general disregard with health and the fact that sex became a certain kind of currency to be exchanged for drugs. Both studies are relevant since they demonstrate some unknown aspects about the reality of those inmates.
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5

Weber, Sérgio. "ASPE/MSC." Florianópolis, SC, 2005. http://repositorio.ufsc.br/handle/123456789/102368.

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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Ciência da Computação
Made available in DSpace on 2013-07-16T00:46:12Z (GMT). No. of bitstreams: 1 224905.pdf: 5536445 bytes, checksum: 9c37dced0f425adf455a3f6d0fa8b466 (MD5)
Segundo dados do ministério da ciência e tecnologia (MCT), o mercado brasileiro na área de software é composto predominantemente por micro e pequenas empresas (MPEs), cujos processos são executados geralmente de modo informal, improvisado e com pouca visibilidade. Esse cenário gera uma série de dificuldades a essas organizações, tornando-as enfraquecidas para competir no mercado e crescerem. Uma alternativa para a mudança desse quadro é o investimento em estabelecimento de processos sistemáticos de software, já que a qualidade dos produtos desenvolvidos são altamente influenciados pelos seus processos de produção. Nesse contexto, o presente trabalho propõe e avalia a abordagem ASPE/MSC (Approach for Software Process Establishment in Micro and Small Companies), cujo objetivo é estabelecer processos de software em MPEs, considerando suas características e limitações. Seu desenvolvimento está baseado em estudos sobre estabelecimento de processos de software, na adaptação de técnicas e abordagens existentes nessa área de pesquisa e na experiência em modelagem de processos de software, obtida pelo LQPS - Laboratório de Qualidade e Produtividade de Software da UNIVALI e pelo Grupo de Qualidade de Software do centro GeNESS. A avaliação da abordagem é realizada por meio de dois estudos de caso, executados em duas pequenas empresas de software em Florianópolis/SC. Os principais resultados do trabalho são a abordagem ASPE/MSC e as experiências obtidas com a execução dos estudos de caso, que incluem dados qualitativos e quantitativos. A abordagem ASPE/MSC está documentada na forma de um guia, que descreve detalhadamente as atividades que a compõem, os papéis envolvidos na execução das respectivas atividades, templates de documentos para auxiliar na sua aplicação e diretrizes para sua execução num contexto real. A perspectiva do trabalho é auxiliar micro e pequenas empresas de software a se fortalecerem no mercado, através da melhoria contínua dos seus processos. Com isso, espera-se que, MPEs possam alcançar gradativamente patamares mais elevados de qualidade, produtividade e competitividade.
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García, Sánchez Ernesto. "Caracterización hidrogeológica de los acuíferos de Aspe y La Horna (Aspe, Alicante)." Doctoral thesis, Universidad de Alicante, 2016. http://hdl.handle.net/10045/59234.

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7

Huber, Mark M. "Legitimacy and Hafez al-Asad." Thesis, Monterey, California. Naval Postgraduate School, 1992. http://hdl.handle.net/10945/23943.

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Approved for public release; distribution is unlimited.
It has, been taken for granted that Hafez al-Asad relies exclusively upon an iron fist to perpetuate the survival of his regime. Close scrutiny of Asad's presidency, however, betrays the inadequacy of this explanation. In fact, Syria's conflict with Israel is the primary legitimizing agent for Asad's minority-Alawi regime, and it is because of this conflict that Asad's regime has endured. Consequently, the absence of a militant confrontation with Israel poses risks which the present Syrian leadership has been unwilling to assume. Furthermore, this condition acts as a restraint upon certain types of foreign policy activities and initiatives which Asad might otherwise elect to pursue. The reality of Israel's legitimizing function has specific relevance to U.S. foreign policy vis-a-vis both Syria and Israel, particularly regarding the peace process. Hafez al-Asad, Syria, Israel, Baath party, Pan-Arabism, Palestinians, 1973 War, Golan Heights, Muslim Brethren, Lebanon, Gulf crisis
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Johansson, Martin. "Hårdgörning av Asp." Thesis, Växjö University, School of Technology and Design, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:vxu:diva-2291.

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Hårdgörning av trä syftar till att göra materialet hårdare. Detta går att åstadkomma genom komprimering, det komprimerade materialet tenderar dock att återgå mot sin ursprungsform då materialet utsätts för fukt om ingen låsning sker. Låsningen kan ske mekaniskt i en treskiktskonstruktion som motverkar träets fuktrörelser. Dessa skivor har dock visat sig vara instabila och tidigare försök har uppvisat deformationer i form av kupning. Denna undersökning syftar till att utreda förutsättningarna för att använda komprimerad asp samt att ta fram underlag för framtagning av en kommersiell produkt.

Praktiska tester har genomförts för att studera hårdhet, återfjädring och densitet hos det komprimerade virket. Vidare har treskiktsskivor tagits fram för att testa om det går att få en stabil konstruktion genom att variera tjockleken på spärrskiktet. Även skillnader i acklimatiseringstid och fuktkvotens inverkan för virkets återfjädring har studerats.

Resultaten visar på att aspens hårdhet efter komprimering blir i samma nivå som bok och ask, en låg fuktkkvot vid komprimering av materialet ger en låg återfjädring, komprimerat material har en längre acklimatiseringstid till jämviktsfuktkvot och materialet går att låsa tvärs fiberriktningen i en treskiktskonstruktion.


To make wood harder it can be densified. This can be achieved by compression, the compressed material tends to return to the original shape when it is exposed to moisture fluxations and if no form of fixation occurs. The fixation can be done mechanically in a three-layer panel construction which reduce movements in the timber. These panels have been found to be unstable and previous attempts have shown deformations in form of cupping. This study aims to evaluate the conditions for using compressed aspen wood, and to provide a basis for development of a commercial product.

Practical tests have been conducted to study hardness, springback and density of the compressed wood. In addition, three-layer panels have been developed to test whether it is possible to have a stable construction by varying the thickness of the buttom layer. Differences in acclimatisation time and the moisture contents effect on the springback has aslo been studied.

The results show that the hardness of aspen wood after compression is in equal levels with beech and ashes, a low moisture contet provides a low springback, compressed timber have a longer time for acclimatisation and it is possible to fixate the material in the direction across the grain in a three-layer panel construction.

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Hrbáček, Martin. "Genarátor ASP stránek." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2008. http://www.nusl.cz/ntk/nusl-217610.

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Submisson of my project is identification with background ASP.NET and realization program for creating ASP pages based on database structure. I began with my study from Microsoft .NET Framework, which provides all tools and technologies for creating .NET aplications. I described features a structure ASP.NET. I followed also, for my project, very important tool ADO.NET, which contains rich group of functions for manipulation with lot of date types. In text I also described object model of this tool. One of parts is about very important ObjectDataSource class. Next part of my project is devote to SQL database and tools for managing and manipulation. One chapters is devote to System register, which serve for database description. Next is described some of realization this type of program. My work is finished by description of my program. Here are aplications of mentioned technologies, which together forms web aplication and her output is web page for data presentation.
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Hasan, Pipip Ahmad Rifai. "The political thought of Muhammad Asad." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape15/PQDD_0006/MQ39932.pdf.

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Thompson, Sydney. "Teaching about Autism Spectrum Disorder (ASD)| University Student's Understanding of ASD." Thesis, Southern Illinois University at Edwardsville, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10789824.

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Limited research exists on what the general population knows about ASD. Previous research that does exist shows that there is variability in what people believe as symptoms, causes, and treatments of ASD. The purpose of this study was to examine the effect that ASD training has on university students’ general knowledge about ASD. One hundred two college-aged students were randomly assigned to one of three conditions: Myths and Facts condition, Myths condition, or Control condition. Pretest and posttest data were collected to examine the likeliness of participants correctly identifying general knowledge of ASD. A mixed-model ANOVA and independent samples t-tests showed that from pretest to posttest, an ASD Myths and Facts Presentation as well as an ASD Myths Presentation significantly increased the likelihood of participants correctly identifying knowledge about ASD. Qualitative data was also examined regarding the percentage of participants answering in agreement to each question from pretest to posttest.

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Firth, Alison. "ASD and sibling relationships." Thesis, Bangor University, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.568971.

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Impairments associated with individuals with Autism Spectrum Disorder (ASD) have led to concerns that their typically developing brothers and sisters will be adversely affected. A literature review was conducted to examine sibling relationship quality for children and young people with a brother or sister with Autism Spectrum Disorder (ASD). A systematic review of the literature yielded ten studies. A number of methodological issues were highlighted, thus there were few studies from which to draw firm conclusions regarding affective and interactive -' . sibling relationship quality and ASD. The review highlights the need for an operational definition of relationship quality, and the development of methodology for future research. The empirical study considered the perceptions and experiences of eight parents regarding their children's sibling relationships where one child had received a diagnosis of ASD. A wealth of information was derived from semi-structured interviews and analysed using Interpretative Phenomenological Analysis (IPA). Five main themes were identified: i) Perceptions of closeness, ii) Perceptions of change, iii) Perceptions of responsibility, iv) Impact of ASD, and v) Perceptions of children's gains from the relationship. The findings provide some insight into parents' views of sibling relationship quality from the perspective ofthe child with ASD, as well as their children's overall sibling relationship quality, which may be important in informing further research projects. Implications for practice include an awareness of issues raised by families when working systemically. Future research and theory development and implications for clinical practice are considered in the final section of this thesis. 2
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Vilímek, Jan. "Konverze ASP do ASP.NET." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2007. http://www.nusl.cz/ntk/nusl-236849.

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The goal of this dissertation is to implement an application for ASP to ASPX conversion. The ASP pages should be written in the VBScript language, the target language for ASPX will be C#. The application is developed on the .NET platform. The conversion process should be automatic. There should be no need to alter the converted files by a programmer. The first part of this dissertation introduces the whole problematic. It shows also current solutions. The next part is the analysis and the design of the application itself. The main part of this dissertation is the VBScript grammar conversion, problems while conversion and its solving.
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Lee, Lee, and 李莉. "The ASPP family in gestational trophoblastic disease." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B40738486.

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Lee, Lee. "The ASPP family in gestational trophoblastic disease." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B40738486.

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Zhou, Y. "Developmental functions of Drosophila ASPP and RASSF8." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1456620/.

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Epithelial cells are connected to each other via intercellular junctions, which are established, remodelled and maintained by a complex molecular machinery. Aberrant regulation of cell-cell junctions leads to loss of tissue organisation and is a hallmark of cancer formation. Although many kinases that regulate the phosphorylation of junctional proteins have been described, much less is known about the reversal of phosphorylation by phosphatases. This thesis investigates the role of Drosophila ASPP, a scaffold protein that is localised at adherens junctions, as a regulatory subunit for PP1s. In vitro, ASPP can bind to PP1 using its RVXF motif and SH3 domain. In vivo, ASPP co-localises with the PP1a96A and PP1b9C isoforms at cell-cell junctions in the pupal retina and ASPP function is at least partially dependent on its ability to bind to PP1. Furthermore, ASPP can recruit two additional coiled coil containing scaffold proteins, RASSF8 and Ccdc85, to form trimeric complexes with PP1a96A. ccdc85 mutants that were generated in this work have a rough eye, similar to ASPP mutants, suggesting a similar function in vivo. Two potential substrates for the ASPP/PP1 complex were tested: (1) Yki a transcriptional co-activator that is part of the Hippo pathway and (2) Baz, a scaffold protein that is required for cell polarity. Although no evidence for dephosphorylation of Yki was found, Baz can be dephosphorylated in vitro. The well-described aPKC phosphorylation site (S980) and five additional serine/threonine residues are strongly dephosphorylated by ASPP/PP1. My work also identified three potential regulators/scaffolds of ASPP/PP1. The Hippo pathway kinase Wts can phosphorylate RASSF8, the E3 ubiquitin ligase Sina can ubiquitylate and degrade ASPP and the junctional protein Magi can associate with RASSF8 at adherens junctions. Finally, novel potential regulators, scaffolds or substrates of the ASPP/PP1 complex were identified through AP-MS experiments and tested for their ability to modulate the ASPP depletion phenotype in vivo.
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Larsson, Camilla. "Ritningar och underhållsplaner på bostadshus vid Aspa Gård." Thesis, Linköpings universitet, Institutionen för teknik och naturvetenskap, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-95356.

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Året var 1942 då egendomen Löfstad slott tillföll Svenska riddarhuset och Östergötlands Fornminnes‐ och Museiförening (nuvarande Östergötlands länsmuseum). Den sista ägarinnan på slottet, Emily Piper, testamenterade egendomen och ville med testamentet bevara slottet för att visa upp det för allmänheten som ett ”verkligt typiskt exempel på ett adligt hem på ett gammalt svenskt gods under 1800‐talet”. I dag förvaltar företaget Linköpings skogstjänst egendomen som förutom slottet består några tusen hektar jord‐ och skogsmark samt 39 bostadshus och ett 60 tal olika ekonomibyggnader. Flertalet gårdar är i dag utarrenderade men till årsskiftet kommer en gård, Aspa Gård tillbaka till förvaltaren som tar över ansvaret för uthyrning och underhåll. I mitt arbete har jag tagit fram planritningar, fasadritningar och underhållsplaner för tre bostadshus som är belägna på Aspa Gård. För att få underlag till ritningar har husen mäts upp både invändigt och utvändigt. För underhållsplanerna har husen även besiktigas okulärt. Utifrån resultatet av besiktningen har husens skick kunnat fastställas och underhållsplaner upprättas. Det visade sig att husens underhåll har varit bristfälligt under många år och att det behövs omfattande renoveringsåtgärder på husen. Det viktigaste är att åtgärda tak, fasad samt våtutrymmen.
The year was 1942 when the properties Löfstad accrue to Svenska riddarhuset and Östergötlands Fornminnes‐ och Museiförening (current Östergötlands länsmuseum). The last owner, Emily Piper, donated the property and wish to preserve the castle in order to sensible up it pursues the general public that one “ really typical examples of a noble home on an old Swedish goods during 1800‐century”. Today, Linköpings skogstjänst carried out the property that apart from castle consist some thousand hectares land‐ and woodland and 39 residential building and amount of economy buildings. The majority farms are today lease to farmers but to the turn of the year comes a farm, Aspa Gård back to the manager that takes over the responsibility for leasing and maintain. In my work I have produce drawings and maintaining plans for three residential building that is located on Aspa Gård. The houses have been measured up both inside and outside to get basis to blueprints. For the maintaining plans, the houses have also been inspected. On the basis of the results of the´inspection can the housesʹ condition be known and maintaining plans to be established. The housesʹ maintain has been inadequate during many years and that it is needed extensive renovate on the houses. The most important to deal with is the ceilings, facades and bathrooms.
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McCoy, Elizabeth. "Tenseless clauses : AspP and the case-licensing of subjects." Thesis, University of York, 2001. http://etheses.whiterose.ac.uk/13994/.

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Wille, Marc. "Hur möter skolan elever med ASD?" Thesis, Uppsala universitet, Institutionen för pedagogik, didaktik och utbildningsstudier, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-220048.

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Följande studie undersöker hur grundskolan bemöter elever med diagnosen Autism Spectrum Disorder (ASD). Här visas det vilka möjligheter det kan finnas för barnet med autism att bli rumsligt inkluderade i skolans verksamhet. Perspektivet är utifrån sju pedagogers erfarenheter och synsätt på inkluderingen av elever med autism. Två av pedagogerna har elever med autism inkluderade i klasserna medan pedagogerna i två andra verksamheter arbetar utifrån särskilda undervisningsgrupper som är knutna till skolan. Undersökningen fokuserar på pedagogernas erfarenheter av och synsätt på två olika typer av inkludering, pedagogisk respektive social inkludering. Med pedagogisk inkludering menas hur pedagogerna anpassar den rumsliga miljön efter elevens behov och vilka konsekvenser som uppstår i deras val. Det finns två verksamheter som inkluderar elever med ASD i de stora klasserna och tar delvis hjälp av resurspersoner. De har speciella sittplatser, timplaner och är indelade i olika nivågrupperingar. En verksamhet arbetar med en liten undervisningsgrupp av fyra barn som har en egen klassrum som beskrivs som en avskalad miljö med så lite störningar som möjlig runt eleven. Den fjärde verksamheten är en särskild undervisningsgrupp för elever med inlärningssvårigheter och utomstående personer beskriver klassrummen som tråkiga men finns det även tankar kring det. Den sociala inkluderingen handlar däremot om samspelet och kommunikationen mellan eleverna och pedagogen men även med andra elever. Det arbetet ställer pedagogerna inför stora utmaningar och samtal, positiv förstärkning och sociala lekar anses som några av verktygen för att fodra det sociala samspelet. En annan sak som tas upp i studien är hur balansen ska hanteras mellan det sociala arbetet och att nå kunskaperna som är skrivna i läroplanen för grundskolan (Lgr11). Några av verksamheterna beskriver att deras elever med ASD klara kunskapsmålen utan problem medan det finns två fall där elever har stora problem med de kraven som ställs av regeringen.
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Cernyw, Ela. "Group interventions for adults with ASD." Thesis, Bangor University, 2015. https://research.bangor.ac.uk/portal/en/theses/group-interventions-for-adults-with-asd(5c72b75b-da67-4166-a568-55bdf4e950a0).html.

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The literature review explored group based psychosocial interventions for adolescents and adults with ASD. The interventions detailed in the review addressed many of the reported difficulties of ASD (i.e. social interaction, communication skills, and managing emotional distress). Fifteen studies met the inclusion criteria and almost all studies (n = 14) reported improvements in most or all of their targeted outcomes. Our ability to assess the overall benefit of group based psychosocial interventions was limited, due to small sample sizes, variation in study qualities, and the heterogeneous nature of the interventions. Research in this field would benefit from moving in a coherent direction, with researchers developing an intervention and evaluating its effectiveness in large scale controlled studies, rather than numerous researchers publishing pilot or small scale studies. The empirical paper described a thematic analysis of participants with ASD (n=4) and facilitators’ (n=2) experiences of a social skills intervention. Richly detailed accounts from participants and facilitators described a broad range of individual and group based processes, and allowed a comparison of multiple perspectives. An overarching concept of separate togetherness was identified in the data, which refers to the shared but individual learning experience within and between the participants and the facilitators. Both papers highlight the challenge of generalisation of skills when working with individuals with ASD, and the difficulty of addressing the individual needs of participants in a group intervention. The results suggest that group based psychosocial interventions show promise, however further, longer-term, exploration is needed in order to consolidate the evidence base. The final paper examines the contributions made to theory and clinical practice, whilst outlining areas requiring further research.
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Fish, Jennifer. "The evolution of neuronal progenitor cell division in mammals: The role of the abnormal spindle-like microcephaly associated (Aspm) protein and epithelial cell polarity." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2007. http://nbn-resolving.de/urn:nbn:de:swb:14-1184837029919-80275.

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Among mammals, primates are exceptional for their large brain size relative to body size. Relative brain size, or encephalization, is particularly striking among humans and their direct ancestors. Since the human-chimp split 5 to 7 million years ago, brain size has tripled in the human lineage (Wood & Collard 1999). The focus of this doctoral work is to investigate some of the cell biological mechanisms responsible for this increase in relative brain size. In particular, the processes that regulate symmetric cell division (ultimately generating more progenitors), the constraints on progenitor proliferation, and how neural progenitors have overcome these constraints in the process of primate encephalization are the primary questions of interest. Both functionally analyses in the mouse model system and comparative neurobiology of rodents and primates are used here to address these questions. Using the mouse model system, the cell biological role of the Aspm (abnormal spindle-like microcephaly associated) protein in regulating brain size was investigated. Specifically, Aspm function in symmetric, proliferative divisions of neuroepithelial (NE) cells was analyzed. It was found that Aspm expression in the mouse neuroepithelium correlates in time and space with symmetric, proliferating divisions. The Aspm protein localizes to NE cell spindle poles during all phases of mitosis, and is down-regulated in cells that undergo asymmetric (neurogenic) cell divisions. Aspm RNAi alters the division plane in NE cells, increasing the likelihood of premature asymmetric division resulting in an increase in non-NE progeny. At least some of the non-NE progeny generated by Aspm RNAi migrate to the neuronal layer and express neuronal markers. Importantly, whatever the fate of the non-NE progeny, their generation comes at the expense of the expansion of the proliferative pool of NE progenitor cells. These data have contributed to the generation of an hypothesis regarding evolutionary changes in the regulation of spindle orientation in vertebrate and mammalian neural progenitors and their impact on brain size. Specifically, in contrast to invertebrates that regulate the switch from symmetric to asymmetric division through a rotation of the spindle (horizontal versus vertical cleavage), asymmetric NE cell division in vertebrates is accomplished by only a slight deviation in the cleavage plane away from the vertical, apical-basal axis. The requirement for the precise alignment of the spindle along the apical-basal axis in symmetric cell divisions may have contributed to selection on spindle “precision” proteins, thus increasing the number of symmetric NE cell division, and contributing to brain size increases during mammalian evolution. Previous comparative neurobiological analyses have revealed an increase in basally dividing NE cells in the brain regions of highest proliferation and in species with the largest brains (Smart 1972a,b; Martinez-Cerdeno et al. 2006). The cell biological characteristics of these basally dividing cells are still largely unknown. We found that primate basal progenitors, similar to rodent apical progenitors, are Pax6+. This suggests that primate basal progenitors may share other properties with rodent apical progenitors, such as maintenance of apical contact. Our previous finding that artificial alteration of cleavage plane in NE cells affects their ability to continue proliferating supports the hypothesis that the apical membrane and junctional complexes are cell fate determinants (Huttner & Kosodo 2005). As such, the need to maintain apical membrane contact appears to be a constraint on proliferation (Smart 1972a,b; Smart et al. 2002). Together, these data favor the hypothesis that primate basally dividing cells maintain apical contact and are epithelial in nature.
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Jiang, Lili, and 蒋莉莉. "Roles of ASPP family and FBI-1 in ovarian cancer." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45458893.

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23

Schmeiser, Katja. "Expression of apoptosis specific proteins (ASPs) during apoptosis and autophagy." Thesis, University of Birmingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.394165.

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ASPs (apoptosis specific proteins) are a protein family with a molecular weight betwccn 20K and 140K. Expression has been found mainly in apoptotic cells. However, some family members have also been detected in viable cells. It has been confirmed by confocal microscopy that these proteins are predominately located along the actin cytoskeleton vvith particular high concentrations corresponding to a-actinin. A gene encoding the 32K ASP component has been cloned from a human foetal liver expression library. This 32K ASP protein (termed Apg5L; ApgS [autophagy 5. S. cerevisiae]-like, human [h]Apg5; Apg5) is homologues to the product of the yeast Apg5 gene. In yeast. Apg5 is essential for a novel protein conjugation system during autophagy. It is believed that members of the ASP family (i.e. 32K ASP/Apg5L) are involved in a similar conjugation system in mammals. This assumption is based on the observation that polyclonal antibodies raised against ApgSL recognise a set of proteins with co-localise with members of the ASP family detected by immunofluorescence.
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24

Burkley, Benjamin M. "Molecular evolution of ASPM exon 18 indel locus in primates." [Gainesville, Fla.] : University of Florida, 2004. http://purl.fcla.edu/fcla/etd/UFE0005261.

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25

Boddy, Christopher Ryan. "Adult Separation Anxiety Disorder (ASAD): Developmental Implications and Clinical Correlates." NSUWorks, 2013. http://nsuworks.nova.edu/cps_stuetd/11.

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Separation anxiety (SA) is often thought to occur exclusively during childhood; however, recent research has identified a putative diagnosis of Adult Separation Anxiety Disorder (ASAD), which mirrors separation anxiety often exhibited during juvenile years. Several studies have suggested that exposure to certain parental features during childhood may lead to the development of ASAD, including parental depression, parental anxiety, and parental overprotection. This study aimed to address these hypotheses by examining retrospective report of exposure to specific parental features among a population of adults diagnosed with ASAD and to elucidate important clinical correlates related to ASAD, such as impairment in daily functioning. The present study used a survey design which was nationally representative and based on the National Comorbidity Survey-Replication and which consisted of a population of 436 participants with a lifetime diagnosis of ASAD and 2,438 participants with no history of a DSM-IV diagnosis. Participants were asked to respond retrospectively about their exposure to the above-mentioned parental features during childhood. Chi square tests revealed that participants with a diagnosis of Adult Separation Anxiety Disorder endorsed exposure to significantly more parental anxiety and parental depression. Additionally, chi square tests indicated that these individuals reported more features related to preoccupied attachment. Using survey participants with a diagnosis of ASAD or subclinical levels of ASAD, the effects of treatment on daily functioning, as well as the level of functional impairment associated with ASAD was measured. Individuals with a history of treatment for ASAD reported significantly more functional impairment in the area of work than those without treatment. An independent-samples t-test revealed that respondents with a diagnosis of ASAD endorsed significantly more parental overprotection than did respondents with no diagnosis. Last, the continuity hypothesis, which states that juvenile symptoms of separation anxiety continue into adulthood, was explored. A chi square goodness-of-fit test was used to determine whether these data fit the continuity hypothesis model. Results did not support the continuity hypothesis, as the number of participants who endorsed childhood separation anxiety was significantly lower than the expected count. Future research should aim to identify efficacious treatments for ASAD, as few studies to date have demonstrated effective means of treating patients with this disorder.
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石川, 久美, K. ISHIKAWA, 克彦 鈴木, K. SUZUKI, 一. 寺井, H. TERAI, 容子 加藤, et al. "アドバンスト・サイエンス・プロジェクト(ASP)学びの杜(V アドバンスト・サイエンス・プロジェクト(ASP)の取り組み)." 名古屋大学教育学部附属中学校 : 名古屋大学教育学部附属高等学校, 2008. http://hdl.handle.net/2237/12788.

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Torstensson, Janina. "MVC-mönster i ASP-NET : Projektblogg bygghemma.se." Thesis, Linnéuniversitetet, Institutionen för datavetenskap, fysik och matematik, DFM, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-12566.

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28

Homs, Raubert Aïda 1983. "Epigenetic alterations in autism spectrum disorders (ASD)." Doctoral thesis, Universitat Pompeu Fabra, 2015. http://hdl.handle.net/10803/403885.

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The aetiology of autism spectrum disorders (ASD), a group of neurodevelopmental conditions with early onset, characterized by social and communication impairment and restricted interests, is unknown in about a third of the patients. The intense research done over the past decade has revealed a genetic contribution, while the epigenetic contribution barely begins to show. The epigenetic marks can exert an effect in gene expression without altering the underlying genetic sequence. In turn, these marks can be impaired by genetic mutations in their target sequence. Therefore, research in genomic, epigenomic and transcriptomic fields will provide convergent information to unravel the causes of ASD, necessary to establish improved diagnostic protocols and therapeutic strategies, allowing an earlier diagnosis and personalized treatment crucial for a better prognosis. Our data reveal variants associated to the phenotype which shows genetic-epigenetic interplay along with gene expression consequences. It also reveals region epigenetic variants, which follow a polygenic or complex model. Finally, we found ASD genotype-specific epigenetic marks. In the future, the progress in cost-efficiency technologies assessing epigenomics, and the availability of a reference epigenome in various tissues and cell types will provide the background to set a step-forward in establishing the developmental stage, cell types and tissues involved in the epigenetic mechanisms of the disorder.
L'etiologia dels trastorns de l'espectre autista (TEA), un grup de malalties del neurodesenvolupament d’aparició primerenca caracteritzades per problemes de comunicació, relació social, i per la presencia d’interessos restringits, és desconeguda per un terç dels individus afectats. La intensa investigació feta durant l'última dècada ha revelat una gran contribució genètica en aquesta malaltia, mentre que de l’epigenètica tot just es comença a evidenciar. Les marques epigenètiques, sense alterar la seqüència genètica subjacent, tenen un efecte en l'expressió dels gens. A la vegada, aquestes marques epigenètiques es poden veure afectades per mutacions genètiques a la seqüència. Així doncs, la recerca en genòmica, epigenòmica i transcriptòmica proporcionarà informació convergent per determinar les causes dels TEA, indispensable per establir millores en els protocols de diagnòstic i en estratègies terapèutiques, facilitant el diagnòstic precoç i el tractament personalitzat, crucial per a un millor pronòstic. Les nostres dades mostren que hi ha alteracions genètiques i epigenètiques associades al fenotip, que interactuen i tenen conseqüències sobre l’expressió gènica. També hem trobat regions amb alteracions epigenètiques, que sembla que contribueixen de manera additiva i seguint un model complex. Finalment, trobem marques epigenètiques específiques de grups de genotips TEA. En el futur, la millora de les tecnologies disponibles per avaluar l’epigenòmica, i la disponibilitat d'un epigenoma de referència en diversos teixits i tipus cel•lulars, serviran com a base per fer un pas cap endavant en l'establiment de l’etapa del desenvolupament, dels tipus cel•lulars i els teixits involucrats en els mecanismes epigenètics del trastorn.
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Ingemarsson, Peter. "Säkerhetsaspekterna i Application Service Provider (ASP)-lösningar." Thesis, University of Skövde, Department of Computer Science, 2001. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-549.

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Bakgrunden till denna undersökning är den växande ASP-marknaden samt säkerhetsproblematiken som finns i en ASP-lösning.

Syftet är att utreda de säkerhetsaspekter som bör beaktas vid en Application Service Provider (ASP)-lösning. Olika aspekter har samlats in dels från litteraturen och dels med hjälp av intervjuer. En uppdelning har gjorts med avseende på mål, hot och kontroll. De mål som först och främst behandlas är de som berör integritet, konfidentialitet och tillgänglighet. De hot som tas upp och som delvis är specifika för en ASP-lösning är ASP-företagets ekonomi och personal samt kommunikationskanalen mellan ASP-företag och ASP-kund. Kontroller som tas upp är med hänsyn till hoten ovan bland annat kontroll av ASP-företagens ekonomi, att personalen kontrollerats samt kontroll av kommunikationsförbindelserna mellan ASP-företaget och kunden. Resultatet av undersökningen är bland annat tio kontrollpunkter med de viktigaste frågorna som en blivande ASP-kund bör ställa till ASP-företaget.

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Johannessen, Nikolay, and Jonas Kihlström. "Konvertering av webbapplikationer från ASP till ASP.NET." Thesis, Karlstad University, Division for Information Technology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-108.

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This dissertation is carried out on behalf of Two i Sverige AB. Two is a consulting agency which primarily works with the development of homepages. The company has several of its complete applications written in ASP e.g. the development of homepages. At the agency they have seen benefit migrating these applications from ASP to the more flexible environment ASP.NET. The motivation for the migration is that the company hopes that by using this technique they will be able to shorten the delivery time of completed products to customers and to develop homepages created in a more modular way. All of Two’s applications are today written in ASP. The company has requested documentation of the migration process to save time and to create a common standard for forthcoming migrations.

This dissertation describes the migration process of two applications made by “Two i Sverige AB” developed for the Internet. Also included is documentation of how the migration process should be performed.

The result of the project is two migrated applications that work with the .NET framework and have the same functionality as the original applications. The documentation of the migration process has resulted in a homepage that is situated on the company’s intranet.


Arbetet som denna uppsats beskriver är utfört på uppdrag av Two i Sverige AB. Two är ett konsultbolag som i första hand arbetar med utveckling av hemsidor. Företaget har flera av sina färdiga applikationer för bl.a. uppbyggnad av hemsidor skrivna i språket ASP. Man har på företaget sett vinster med att konvertera dessa applikationer från ASP till den mer flexibla miljön ASP.NET. Motivering till konverteringen är att företaget anser att man efter konvertering till ASP.NET kommer att kunna förkorta leveranstiden av färdiga produkter till kund, samt lättare skapa hemsidor uppbyggda med moduler. Idag är Two:s samtliga applikationer skrivna i ASP. Företaget vill även ha en dokumentation över konverteringsprocessen för att spara tid och att skapa en gemensam standard inför kommande konverteringar.

Denna uppsats beskriver konverteringsprocessen för två av företagets applikationer och även dokumentation för hur konverteringsprocessen bör se ut.

Resultatet av arbetet är två konverterade applikationer som fungerar på .NET ramverket och har samma funktionalitet som de ursprungliga applikationerna. Dokumentationen av konverteringsprocessen har resulterat i en hemsida som är belägen på företagets intranät.

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31

Wheeler, John J. "Designing Effective Instruction for Children with ASD." Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/etsu-works/264.

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32

Johnson, Jeremy Charles. "Designing an electron learning Website using ASP." CSUSB ScholarWorks, 2004. https://scholarworks.lib.csusb.edu/etd-project/2528.

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The primary purpose of this project is to develop an online forum to facilitate communication among educators, parents, and students resulting in an open environment for more informed decisions by all those involved in the educational process. The second purpose is the personal development of an electronic learning application using online tools needed for an effective online learning environment that will cost the school district little or nothing.
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33

Olsson, Alexandra, and Malin Fahlén. "Datahantering vid dynamiska webbteknologier : PHP eller ASP?" Thesis, Blekinge Tekniska Högskola, Institutionen för programvaruteknik och datavetenskap, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-3795.

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The last couple of years the use of Internet has changed in the way that the web pages can be affected of the person that uses them. Because of this dynamic Internet technology has emerged that makes it possible for a web page to receive information from a web browser. This information can then be used to affect the appearance of the page. PHP and ASP are two dynamic Internet technologies. They are integrated in HTML and are executed on the web server. E-commerce and search pages are example of this kind of web pages. The purpose of this examine thesis is to find out which of the technologies that are best suited when information needs to be stored on the web server. The thesis turns mostly to programmers that are planning to create a web application for this purpose. The analysis, which consists of three main questions, is based on a literature study and on a web application that has been developed. The result of the analysis is that ASP is more complicated because more external components are included and a lot of objects has to be created. PHP is a much more structured technology and is simpler constructed. Our conclusion of this thesis is that the functionality within both technologies are basically the same. But because PHP´s code is constructed much simpler this technology is more suited for storing. De senaste åren har användningen av Internet ändrats på så sätt att webbsidor ska kunna påverkas av den som använder sig av dem. Därför har något som kallas dynamisk webbhantering skapats, vilket gör det möjligt för en webbsida att ta emot information från en webbläsare. PHP och ASP är två teknologier inom dynamisk webbhantering. De integreras i HTML-kod och exekveras på webbservern. Exempel på sådana sidor är e-handelsidor och söksidor. Detta examensarbete har gjorts för att ta reda på vilken av teknologierna som passar bäst när lagring av data på webbservern krävs. Arbetet vänder sig främst till programmerare som planerar att skapa en webbapplikation för detta ändamål. Analys har gjorts utifrån en frågeställning baserad på litteraturstudier och på programmering av en minde webbapplikation. Resultatet av analysen är att ASP, i just de frågor vi tagit upp, är mer komplicerat då externa komponenter blandas in och många objekt måste skapas. PHP är mycket mer strukturerat och enklare uppbyggt. Vår slutsats av arbetet är att funktionaliteten hos de båda teknologierna i stort sett är den samma, men på grund av att PHP är enklare kodningsmässigt sett är det denna teknologi som är bäst lämpad vid datalagring.
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Mak, Chun-yin, and 麥俊然. "Molecular studies on ASPP and FBI-1 in gestational trophoblastic disease." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B47236279.

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Gestational trophoblastic disease (GTD) encompasses a heterogeneous group of trophoblastic lesions. It includes hydatidiform mole (HM), choriocarcinoma (CCA), placental site trophoblastic tumour (PSTT) and epithelioid trophoblastic tumour (ETT). While the latter three are classical malignancies, HM represents abnormal placenta harbouring malignant potential and may develop persistent gestational trophoblastic neoplasm (GTN) requiring therapy. Apoptotic activity and p53 are known to be important in pathogenesis of GTD. However, understanding on the underlying mechanisms is still limited. ASPP1 and ASPP2 are the two tumour suppressor members of the apoptosis stimulating protein of p53 (ASPP) family that stimulate p53-dependent apoptotic pathway. In this study, a differential downregulation of ASPP1 and ASPP2 was observed in GTD, illustrating their distinct roles in the pathogenesis. Results showed that both ASPP1 mRNA and protein levels were significantly downregulated in HM and CCA, when compared with normal placentas by quantitative polymerase chain reaction (qPCR) and immunohistochemistry. Methylation-specific polymerase chain reaction (MS-PCR) in placenta and GTD samples demonstrated a significant correlation between ASPP1 mRNA level and their hypermethylation status (P = 0.024). Most importantly, lower ASPP1 immunoreactivity was found in HM that progressed into persistent GTN than HM that regressed (P = 0.045). Significant correlation was also found between expression of ASPP1 and apoptotic activity (M30 CytoDeath index), p53 and caspase-8 immunoreactivities. In CCA cell lines, namely JEG-3 and JAR, ectopic expression of ASPP1 triggered apoptosis through intrinsic and extrinsic pathways as indicated by an increase in cleaved caspase-9 and Fas ligand protein expression, demonstrating a potent tumour suppressive function through its proapoptotic nature. Downregulation of ASPP2 was mainly detected in choriocarcinoma, the most aggressive form of GTD. In vitro, results showed that ASPP2 was a multi-functional protein with activities not limited to apoptosis stimulation. While activated Src is important in tumour progression, transfection of ASPP2 but not ASPP1 was found to be related to decreased Src-pY416 phosphorylation. This suggested an inactivating effect of ASPP2 on Src. Moreover, this ASPP2-induced inactivation of Src could be abolished by RNA interference (RNAi) with Csk small interfering RNA. Corresponding effect on aggressive behaviors such as cell migration was also confirmed. Hence, loss of ASPP2 in the choriocarcinoma implicates its crucial role in tumourigenesis. FBI-1 is a transcription factor frequently overexpressed in human cancers. Recently, overexpression of FBI-1 in GTD in association with GTN development was also reported. However, its role in GTD pathogenesis remains elucidated at molecular basis. This study unveiled the ability of FBI-1 in contributing overt aggressiveness in GTD. Ectopic FBI-1 expression resulted in decrease in apoptosis and repression of pro-apoptotic genes such as Bak, Fas and caspase-8 at mRNA level in vitro. FBI-1 overexpression also promoted Akt activation as indicated by Akt-pS473 phosphorylation. Interestingly, effect of FBI-1 on cell motility and invasiveness was eradicated in the presence of specific PI3 kinase inhibitor LY294002. This demonstrated that FBI-1 could promote cell migration and invasion through PI3K/Akt signaling. In summary, this study highlighted the effects of dysregulated ASPP and FBI-1 in apoptosis and aggressiveness, implicating their crucial roles in pathogenesis in GTD.
published_or_final_version
Pathology
Doctoral
Doctor of Philosophy
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35

Silva, Michele Oliveira da [UNESP]. "Avaliação sistematizada para professores de alunos com paralisia cerebral (ASPA-PC)." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/102197.

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Made available in DSpace on 2014-06-11T19:31:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2014Bitstream added on 2014-06-13T19:02:14Z : No. of bitstreams: 1 000739997.pdf: 3857913 bytes, checksum: 0e87faa7eaea45e721d05f835cf4ca42 (MD5)
Na área da Educação Especial, a elaboração do planejamento acadêmico depende de uma avaliação prévia das dificuldades e habilidades dos alunos para posteriormente elaborar uma intervenção de acordo com o que foi avaliado. Porém, os professores especializados nem sempre têm disponível um instrumento para avaliar e articular os dados dessa avaliação para uma intervenção pedagógica. No caso específico do aluno com paralisia cerebral, essa dificuldade pode ser atribuída à diversidade de características físicas e cognitivas que devem ser avaliadas e a relação delas com as exigências do meio. Diante disso, o objetivo do presente trabalho foi elaborar e avaliar a aplicabilidade da avaliação sistematizada para professores de alunos com paralisia cerebral (ASPA-PC) para avaliar alunos com paralisia cerebral, matriculados no 1º, 2º e 3º ano do Ensino Fundamental, para subsidiar o planejamento curricular e a estratégias de ensino. O estudo foi elaborado em três etapas. Na primeira etapa foi realizado o Estudo Piloto no qual uma aluna com paralisia cerebral foi avaliada com o uso de um instrumento de avaliação já publicado. A análise dos resultados da primeira etapa subsidiou a elaboração da primeira versão do ASPA-PC na segunda etapa. Ainda nessa etapa, a partir da avaliação de quatorze alunos com o uso do ASPA-PC foi indicado um kit de avaliação e um manual de aplicação que foram utilizados por professoras do atendimento educacional especializado. Os resultados das etapas anteriores definiram a divisão do ASPA-PC em ASPA-PC KIT I para avaliar alunos até o 2º ano e ASPA-PC KIT II para avaliar alunos do 3º ano do Ensino Fundamental, resultando na sua versão definitiva, na terceira etapa. Além do manual e de um roteiro para guiar a conversa inicial entre o avaliador e o professor do ensino regular do aluno, o instrumento foi constituído de cinco Cadernos: 1) Caderno para observação em sala de aula;...
In the area of Special Education, an academic plan development depends on a previous assessment of the difficulties and abilities of students to enable an intervention according to what has been reported. However, specialist teachers do not always have an available tool to assess and articulate the data from this assessment for an educational intervention. In the specific case of students with cerebral palsy, this difficulty can be attributed to the diversity of physical and cognitive characteristics that must be assessed and compared with the demands of the environment. Therefore, the aim of this research was to develop and evaluate the applicability of systematic assessment for teachers of students with cerebral palsy (ASPA-PC) to assess students with cerebral palsy enrolled in 1st, 2nd and 3rd grades of Elementary School, to support curriculum planning and teaching strategies. The study was prepared in three steps: in the first step, a pilot study was conducted when a student with cerebral palsy was assessed with the use of an assessment tool already published in the literature. The results of the first step subsidized the development of the first version of ASPA - PC in the second step. Still in the second step, based on the assessment of fourteen students using ASPA-PC, a kit and an application manual that was used by Special Education teachers and was referred. The results of the previous steps defined the separation of the material in two parts: ASPA-PC and ASPA-PC KIT I to assess students up to the 2nd grade and ASPA-PC KIT II to assess students of the 3rd grade of elementary school resulting in its final version during the third phase. In addition to manual and a roadmap to guide the initial conversation between the evaluator and the non-special education teacher, the tool consisted of five books: 1) Logbook for classroom observation, 2) Notebook for Portuguese Language; 3) Notebook for...
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Silva, Michele Oliveira da. "Avaliação sistematizada para professores de alunos com paralisia cerebral (ASPA-PC) /." Marília, 2014. http://hdl.handle.net/11449/102197.

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Orientador: Eduardo José Manzini
Banca: Lígia Maria Presumido Braccialli
Banca: Jáima Pinheiro de Oliveira
Banca: Olga Maria Piazentin Rolim Rodrigues
Banca: Adriana Garcia Gonçalves
Resumo: Na área da Educação Especial, a elaboração do planejamento acadêmico depende de uma avaliação prévia das dificuldades e habilidades dos alunos para posteriormente elaborar uma intervenção de acordo com o que foi avaliado. Porém, os professores especializados nem sempre têm disponível um instrumento para avaliar e articular os dados dessa avaliação para uma intervenção pedagógica. No caso específico do aluno com paralisia cerebral, essa dificuldade pode ser atribuída à diversidade de características físicas e cognitivas que devem ser avaliadas e a relação delas com as exigências do meio. Diante disso, o objetivo do presente trabalho foi elaborar e avaliar a aplicabilidade da avaliação sistematizada para professores de alunos com paralisia cerebral (ASPA-PC) para avaliar alunos com paralisia cerebral, matriculados no 1º, 2º e 3º ano do Ensino Fundamental, para subsidiar o planejamento curricular e a estratégias de ensino. O estudo foi elaborado em três etapas. Na primeira etapa foi realizado o Estudo Piloto no qual uma aluna com paralisia cerebral foi avaliada com o uso de um instrumento de avaliação já publicado. A análise dos resultados da primeira etapa subsidiou a elaboração da primeira versão do ASPA-PC na segunda etapa. Ainda nessa etapa, a partir da avaliação de quatorze alunos com o uso do ASPA-PC foi indicado um kit de avaliação e um manual de aplicação que foram utilizados por professoras do atendimento educacional especializado. Os resultados das etapas anteriores definiram a divisão do ASPA-PC em ASPA-PC KIT I para avaliar alunos até o 2º ano e ASPA-PC KIT II para avaliar alunos do 3º ano do Ensino Fundamental, resultando na sua versão definitiva, na terceira etapa. Além do manual e de um roteiro para guiar a conversa inicial entre o avaliador e o professor do ensino regular do ... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: In the area of Special Education, an academic plan development depends on a previous assessment of the difficulties and abilities of students to enable an intervention according to what has been reported. However, specialist teachers do not always have an available tool to assess and articulate the data from this assessment for an educational intervention. In the specific case of students with cerebral palsy, this difficulty can be attributed to the diversity of physical and cognitive characteristics that must be assessed and compared with the demands of the environment. Therefore, the aim of this research was to develop and evaluate the applicability of systematic assessment for teachers of students with cerebral palsy (ASPA-PC) to assess students with cerebral palsy enrolled in 1st, 2nd and 3rd grades of Elementary School, to support curriculum planning and teaching strategies. The study was prepared in three steps: in the first step, a pilot study was conducted when a student with cerebral palsy was assessed with the use of an assessment tool already published in the literature. The results of the first step subsidized the development of the first version of ASPA - PC in the second step. Still in the second step, based on the assessment of fourteen students using ASPA-PC, a kit and an application manual that was used by Special Education teachers and was referred. The results of the previous steps defined the separation of the material in two parts: ASPA-PC and ASPA-PC KIT I to assess students up to the 2nd grade and ASPA-PC KIT II to assess students of the 3rd grade of elementary school resulting in its final version during the third phase. In addition to manual and a roadmap to guide the initial conversation between the evaluator and the non-special education teacher, the tool consisted of five books: 1) Logbook ... (Complete abstract click electronic access below)
Doutor
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Piumatti, Matteo. "The interactome of the microcephaly gene ASPM in human cortical cells." Doctoral thesis, Universite Libre de Bruxelles, 2021. https://dipot.ulb.ac.be/dspace/bitstream/2013/322985/8/Table2.xlsx.

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Mutations in the Abnormal Spindle-like Microcephaly-associated (ASPM) gene are the most common cause of primary microcephaly, a rare condition characterized by a severe reduction of brain size at birth. Several studies allowed to identify ASPM as a centrosome and mitotic spindle protein that regulates cell division and spindle orientation. However, little is known about ASPM molecular mechanisms, especially in human neural cells relevant to the disease. In order to decipher the molecular mechanisms of action of ASPM in human corticogenesis, we used co-immunoprecipitation (coIP) followed by mass spectrometry to identify the interactors of ASPM in human HEK cells and human cortical progenitors differentiated from pluripotent stem cells engineered to tag the endogenous ASPM protein. We thus identified and validated 14 ASPM interactors of which 12 are newly reported, and seven are found specifically in neural cells, including the important spindle pole regulator Nuclear mitotic apparatus (NUMA). We then characterized the expression and localization of the identified proteins in human cortical progenitors differentiated from control and isogenic ASPM mutant cells. This revealed that many of the identified proteins are selectively located at the spindle pole, and that this selective localization is disrupted in mutant cells for several of the interactors, in particular the MAP7 domain-containing protein 1 (MAP7D1) and DnaJ homolog subfamily B member 6 (DNAJB6). Our data uncover some of the complex ASPM interactome relevant and specific to human brain development and microcephaly, and suggest that ASPM acts as a major molecular hub at the centrosome and mitotic spindle to control the patterns of cell division of cortical progenitors.
Doctorat en Sciences biomédicales et pharmaceutiques (Médecine)
info:eu-repo/semantics/nonPublished
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Kostova, Simona [Verfasser]. "Functional characterization of a novel p97:ASPL protein complex / Simona Kostova." Berlin : Freie Universität Berlin, 2021. http://d-nb.info/1236572866/34.

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39

Österlund, Ragnar. "Konvertering av CRM-system från ASP till ASP.NET." Thesis, Uppsala University, Department of Information Technology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-89415.

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S2 CRM is a Customer Relations Management system built in classic ASP that runs on a web server servicing customers with varying needs. The purpose of this thesis work is to convert parts of the system to the web development environment ASP.NET. The purpose is also to gain knowledge of the framework itself. The ASP.NET environment is compared with another framework, JBoss Seam, in order to understand the differences and to evaluate the possibility of using another framework than ASP.NET, when building the new system. The outcome of this comparison is that although JBoss Seam is just as good, and in some aspects better than ASP.NET, the use of ASP.NET simplifies the continuation of development on the S2 CRM product. The conversion that was made includes a way to share information between the new and the old system, an implementation of listing and search modules and an approach to the data model and techniques for achieving good performance. Modularity is also an important aspect of the work, and this was achieved by constructing controls that can be used in different contexts, configurable by configuration objects. The result of the thesis is a basic system for listing and searching that can coexist with the original system and that can be built further upon to create a new version of S2 CRM that fully runs on the ASP.NET platform.

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Petersson, Anders, and Toni Sjöö. "Böjhållfasthet och styvhet hos sågat virke av asp." Thesis, Växjö University, School of Technology and Design, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:vxu:diva-1160.

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Med ekonomiskt stöd från Stiftelsen för kunskaps- och kompetensutveckling skall ett lövträprojekt bedrivas under två års tid vid Institutionen för teknik och design på Växjö Universitet. Detta examensarbete ingår som en del av lövträprojektet, där böjhållfasthetsvärden och elasticitetsmoduler för asp tagits fram.

Virket har hållfasthetssorterats visuellt dels efter nordiska T-virkesstandarden INSTA 142, men även efter den brittiska standarden BS 4978-1996. Dessutom har en maskinell hållfasthetssortering av provkropparna genomförts i en böjprovningsmaskin av fabrikatet Cook-Bolinder. För vidare analyser av provkropparna har de körts i en WoodEye skanner som är en maskinell sorterings- och bildinläsningsapparat för sågat och hyvlat virke.

För att bestämma aspens elasticitetsmoduler och böjhållfasthet användes en böjprovningsmaskin av fabrikatet Alwetron. Global och lokal elasticitetsmodul har beräknats utifrån uppmätt nedböjning enligt den Europeiska standarden prEN 408:2003, därefter belastades provkroppen till brott för att få fram böjhållfastheten. Provkropparna har fotograferats innan påförd last, vid begynnande brott och slutligen efter brott. För möjlighet till ytterligare studier av de 150 provkroppar har en databas skapats med samtliga registrerade värden och foton.


With financially supports from the trust fond of the Knowledge Foundation a hardwood project will take part during two years at the School of Technology and Design at Växjö University. This report is one part in this project, were the bending strength and modulus of elasticity were evaluated for aspen.

The specimens were strength classified visually by using the Nordic standard INSTA 142 and by using the British standard BS 4978-1996. A classification was carried out by a bending strength grading machine named Cook-Bolinder. For further analysis the specimens were finally documented with a WoodEye scanner, which is a scanner that inspects and optimises sawn and planed timber.

An Alwetron, a bending strength grading machine, was used to determine modulus of elasticity and bending strength for aspen. Global and local modulus was calculated from the deflection according to the European standard prEN 408:2003. After that the specimens were loaded by tension to their ultimate strength so it was possible to evaluate the bending strength. Photos were taken of the specimens before, during and after loading. For further studies of the 150 specimens all values and pictures were saved.

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Morris, Hannah Christina. "Experiencing support: Parents of children diagnosed with ASD." Thesis, University of Bristol, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.654567.

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The aim of this study was to explore the differentiated experiences of parents who have a child with a diagnosis of ASD and investigate how parental engagement varies with a range of different circumstances, care time-lines and family backgrounds. This study reports data from seven parents who have a child with a diagnosis of ASD. Information was gathered through semi-structured interviews and the use of an events timeline. All data was analysed using Interpretative Phenomenological Analysis. The analysis confirmed that parents have very different experiences across the care-pathway. Some parents experienced a number of critical periods whereby they found it particularly difficult to cope with their child's behaviour. Relationships with professionals were extremely important in building parental confidence across the whole care-pathway. Parents cited that they would like more professionals to develop further care-giving skills in partnership building in order to understand, appreciate and include the parent voice. The study provides recommendations for all professionals and services working with parents who have a child with ASD, in particular the need for professional training in care-giving skills, the importance of providing early intervention in complex cases and the need for additional support at crisis points and during the secondary years.
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Roy, Christian. "La voie ASP/C5L2 dans le métabolisme énergétique." Thesis, Université Laval, 2014. http://www.theses.ulaval.ca/2014/30270/30270.pdf.

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L’obésité est un problème de santé majeur qui se traduit par un déséquilibre du métabolisme énergétique. La protéine stimulant l’acylation (ASP, C3adesArg) est une protéine produite par le tissu adipeux qui stimule la synthèse des triglycérides (TG) et le transport du glucose en se liant à son récepteur C5L2. Nos études précédentes ont établi que les souris avec le gène C3 invalidé (C3KO) sont des souris déficientes en ASP, hyperphagiques, mais de poids normal, affichant une dépense énergétique plus élevée que les souris sauvages. C’est à partir de ces résultats que nous avons basé notre objectif de déterminer le rôle de la voie ASP-C5L2 dans le métabolisme énergétique et cela autant au niveau du cerveau que des organes périphériques. Lors de notre première étude, nous avons observé qu’une injection centrale d’ASP au niveau du troisième ventricule chez des rats Wistar, amenait une diminution de la prise alimentaire et du poids corporel ainsi qu’une augmentation de l’expression d’ARNm du neuropeptide anorexigène pro-opiomélanocortine (POMC) dans la région du noyau arqué. Nos études révèlent également que les niveaux de lipides intramyocellulaires (IMCL) sont augmentés de six fois chez les souris déficientes en C5L2 (C5L2KO) par rapport aux souris sauvages (WT) suite à un régime riche en graisse. De plus, chez les sujets humains, l’expression protéique de C5L2 est réduite chez les patients diabétiques de type 2 par rapport aux témoins obèses. L'entraînement physique a augmenté l’expression protéique de C5L2 et la production d’ASP chez les hommes obèses insulino-résistants. Enfin, nos travaux avec les souris déficientes pour le récepteur du C5a (C5aRKO) montrent qu’après 12 semaines de diète riche en graisse et en sucre (DIO), les souris C5aRKO présentent une diminution du poids corporel ainsi que des dépôts adipeux gonadiques et inguinaux plus petits que leurs homologues WT. Des taux de triglycérides et d'acides gras non-estérifiés plasmatiques plus faibles et une clairance postprandiale plus rapide des triglycérides ont aussi été constatés. Nous avons donc démontré que la voie ASP/C5L2 représente une cible intéressante autant dans le contrôle de la prise alimentaire que dans le métabolisme des acides gras du muscle squelettique.
Obesity is a major health problem that results in an imbalanced energy metabolism. Acylation Stimulating Protein (ASP, C3adesArg) is a protein produced by the adipose tissue, which stimulates triglycerides (TG) synthesis and glucose transport by binding to its receptor C5L2. Our previous studies have shown that mice with a disabled C3 gene (C3KO) are ASP deficient, hyperphagic but of normal weight and demonstrate greater energy expenditure than wild-type mice. From these results, our goal was to determine the role of the ASP-C5L2 pathway in energy metabolism. In our first study, we observed that a central injection of ASP in the third ventricle of Wistar rats brought a reduction in food intake and body weight gain and an increased mRNA expression of the anorexigenic neuropeptide POMC in the region of the arcuate nucleus. Our studies also showed that the levels of intramyocellular lipids (IMCL) were increased six-fold in C5L2-deficient mice (C5L2KO) compared to wild mice (WT) after a high-fat diet. Furthermore, in humans, the protein expression of C5L2 is reduced in patients with type 2 diabetes compared with obese controls. Physical training increased protein expression of C5L2 and ASP output in obese insulin-resistant men. Finally, our work with C5a receptor-deficient mice (C5aRKO) showed that after 12 weeks of a high-fat and high-sugar diet (DIO), the C5aRKO mice exhibited reduced body weight and smaller gonadal and inguinal fat mass than their WT counterparts. This was accompanied with lower plasma levels of non-esterified fatty acids and triglycerides and faster postprandial triglyceride clearance. Our studies have demonstrated that the ASP/C5L2 pathway represents an attractive target in the control of food intake and skeletal muscle fatty acid metabolism.
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43

West, Simon David Collin. "Neutral ASD conformal structures with null Killing vectors." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.611987.

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44

Challa, Varshi. "Accessing timesheets via internet through ASP and ODBC." CSUSB ScholarWorks, 2000. https://scholarworks.lib.csusb.edu/etd-project/1605.

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The purpose of this project is to develop a computerized timesheet application. Using this application, an employee of a company can log onto the company's Web site and fill out a timesheet from anywhere in the world. The project involved automating timesheet data entry and approval procedures using contemporary technologies like Active Server Pages (ASP), JavaScript, VB Script, Component Object Model (COM), Components and Open Database connectivity (ODBC).
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Eliasson, Kent. "University enrollment and geographical mobility : the case of Sweden /." Umeå : Umeå universitet, 2001. http://signum.kb.se/getcode1.asp.

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46

Gulløv, Hans Christian. "From Middle Ages to Colonial Times : archaeological and ethnohistorical studies of the Thule culture in South West Greenland 1300-1800 AD /." Kbh. ; Brenderup : Kommissionen for Videnskabelige Undersøgelser i Grønland = Commission for Scientific Research in Greenland : Geografforlaget, 1997. http://www.dpc.dk/sw2403.asp.

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47

Savoret, Juliette. "ASP, la protéine AntiSens du VIH-1 : étude de ses transcrits et de la réponse anticorps anti-ASP chez le patient." Thesis, Montpellier, 2019. http://www.theses.fr/2019MONTT050.

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Le VIH-1 possède sur le brin antisens de son génome proviral un gène codant pour une protéine nommée ASP (Antisense Protein). Le gène codant pour ASP est uniquement conservé dans le groupe M du VIH-1 responsable de la pandémie, ce qui suggère fortement l’implication d’ASP et de ses transcrits dans la virulence ou la propagation du VIH-1. Plusieurs études ont rapporté l’expression de transcrits antisens du VIH-1 et de la protéine ASP dans des cellules infectées in vitro et ex vivo. Cependant, malgré ces avancées, l’expression d’ASP chez le patient reste contestée, et les informations concernant sa fonction potentielle sont limitées. Afin d’appuyer l’existence d’ASP et de ses transcrits in vivo et d’améliorer notre compréhension de leur expression chez le patient, j’ai étudié les transcrits d’asp et la réponse anticorps anti-ASP dans des échantillons biologiques de patients infectés par le VIH-1. J’ai développé une technique basée sur l’immuno-précipitation de la protéine de fusion nanoluciférase-ASP pour détecter les anticorps anti-ASP, ainsi qu’une technique de RT-qPCR brin-spécifique pour détecter les transcrits d’asp. A l’aide de ces deux techniques, j’ai détecté la présence de transcrits d’asp dans les cellules isolées de 2 patients, et la présence d’anticorps anti-ASP dans le plasma de 4 patients. Chez ces 4 patients, la réponse anticorps anti-ASP fluctue au cours du temps et cible un ou plusieurs épitopes conservés d’ASP. Dans l’ensemble, mes résultats démontrent que le gène asp est exprimé au cours de l’infection par le VIH-1, et confirment l’immunogénicité de différents domaines de la protéine ASP. Bien que l’étude d’un petit nombre de patients ne permette pas d’analyser la protection éventuellement conférée par la réponse anticorps anti-ASP, la compréhension des paramètres immuno-virologiques qui gouvernent les fluctuations de cette réponse anticorps chez le patient pourrait avoir d’intéressantes retombées thérapeutiques
HIV-1 presents a gene encoding for a protein named ASP (AntiSense Protein) on the antisense strand of its proviral genome. The gene encoding ASP is uniquely conserved within the M group of HIV-1 responsible for the pandemic, wich strongly suggests the implication of ASP and its transcripts in HIV-1 virulence or spreading. Several studies reported the expression of HIV-1 antisense transcripts and of ASP protein in cells infected in vitro and ex vivo. Despite these progress however, the expression of ASP in patients remains a subject of debate, and limited information is available on its potential function. To support the in vivo expression of ASP and its transcripts, and to improve our understanding of its expression in patients, I studied asp transcripts and the antibody response anti-ASP in biologic samples from HIV-1 infected patients. I developed a technic based on the immuno-precipitation of a nanoluciferase-ASP fusion protein to detect anti-ASP antibodies, and a strand-specific RT-qPCR to detect asp transcripts. Using both technics, I detected asp transcrits in the cells isolated from 2 patients, and the presence of anti-ASP antibodies in the plasma of 4 patients. In these 4 patients, the anti-ASP antibody response fluctuates overtime and targets one or several conserved epitopes of ASP. Overall, my results demonstrate that the asp gene is expressed during HIV-1 infection and confirm the immunogenicity of different domains of ASP. Even though the study of a small number of patients does not allow assessing the eventual protection conferred by the anti-ASP antibody response, the comprehension of the immuno-virological parameters underlying its fluctuations in patients could have interesting therapeutic implications
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Grantz, Caroline Jennifer. "The Emergence of Triadic Attention in Infant Siblings of Children with an Autism Spectrum Disorder." Scholarly Repository, 2010. http://scholarlyrepository.miami.edu/oa_theses/29.

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Triadic attention, sharing attention with a person about an object or event, typically develops between eight and 12 months of age. This ability facilitates the development of social skills and language through shared exploration of objects and social stimuli. Two key aspects of triadic attention are initiating joint attention (IJA), the use of gaze and gestures to involve another in an experience, and behavioral requesting (IBR), the use of verbal and nonverbal communication to elicit help from another. Both aspects of triadic attention are impaired in individuals with Autism Spectrum Disorders (ASDs), which are characterized by social and communication deficits. The current study investigated the early development of triadic attention in eight- to- 12 month old infants who either have an older sibling diagnosed with an ASD (ASD-Sibs), or have older siblings with no ASD symptomatology (COMP-Sibs). This study examined age-related changes in the frequency of infant-initiated triadic attention using two measures, the Early Social Communication Scales (ESCS) and parent-administered Triadic Play Interaction (TPI). Triadic attention had modest associations between the TPI and the ESCS, with three of the six possible associations significant. At eight months of age, ASD-Sibs initiated significantly fewer IJA bids during the ESCS than COMP-Sibs. At 12 months of age, presence of IBR during the TPI differed significantly by group, with 18 percent of ASD-Sibs initiating a behavioral request, compared to 58 percent of COMP-Sibs. This study demonstrated that infant-initiated triadic attention behaviors differed by group status in both measures used, suggesting these measures offer different and complementary information regarding triadic attention behaviors in ASD-Sibs compared to COMP-Sibs. The use of the TPI in examining triadic attention development in ASD-Sibs is discussed.
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Pullo, Omar Santiago Pillaca. "Produção de L-asparaginase (ASP3) de Saccharomyces cerevisiae expressa em Pichia pastoris." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/9/9134/tde-08112016-120926/.

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A enzima L-asparaginase (ASNase) usada como biofármaco no tratamento de Leucemia Linfoblástica Aguda (LLA) é de origem bacteriana, o que provoca reações imunológicas nos paciente e a ASNase usada no Brasil é importada o que dificulta seu uso por razões de abastecimento e de preço. Por outra parte, dentro dos sistemas de expressão de proteínas recombinantes usados pela biotecnologia, destaca-se a Pichia pastoris, levedura metilotrófica de fácil manipulação, crescimento rápido, alta capacidade de expressão e capaz de realizar modificações pós-traducionais. Neste projeto, o gene de ASNase II de Saccharomyces cerevisiae foi expressa em P. pastoris (Muts), tendo sido analisada a localização da enzima nos meios extracelular, intracelular e espaço periplasmático. Além disso, foram avaliadas diversas condições de crescimento e indução da ASNase em agitador orbital e finalmente foi feito o cultivo em biorreator de 3L operado em batelada. Segundo o analise da expressão, a enzima foi localizada no espaço periplasmático. O crescimento de P. pastoris em diferentes concentrações de glicerol (10,0 - 50,0 g.L-1) mostraram parâmetros cinéticos similares (µmáx = 0,35 h-1; tg= 2,0 h) e o maior fator de conversão de substrato em células (Y x/s = 0,9 g g-1) foi obtido com 10,0 g.L-1 de glicerol. A expressão de ASNase somente ocorreu a 20 °C e melhora com concentrações de metanol acima de 1,0% (v/v), obtendo-se a maior produção da enzima a 3% de metanol durante 48 horas, o que foi corroborado pelo planejamento fatorial fraccionado (3n-k + 2), n = 3 e k =1. Também se observou que o pH de indução, a suplementação com aminoácidos ou casaminoácidos e concentração de glicerol durante a fase de crescimento apresentaram pouca ou nenhuma influência na expressão. Entretanto, as células induzidas imediatamente após o glicerol ter sido consumido melhoraram a produção de ASNase. No cultivo em biorreator, a fase de crescimento foi feita com 10,0 e 40,0 g.L-1-1 de glicerol, e a fase de indução com pulsos de 3,0% (v/v) de metanol durante 120 horas. A maior atividade volumétrica (710,2 U.L-1) de ASNase foi obtida na batelada com 40,0 g.L-1 de glicerol e a atividade periplasmática foi constante durante o tempo de cultivo o que significa que o controle do pH afeta positivamente na expressão de ASNase.
The bacterial L-asparaginase (ASNase) is used as biopharmaceutical in the treatment of acute lymphoblastic leukemia (ALL) and non-Hodgkin lymphoma and because his origin causes immune reactions in patients. The ASNase used in Brazil is imported which hinders its use due to supply availability and price. The methylotrophic yeast Pichia pastoris, is a microrganism easy to handle and with fast growth and high capacity of expression of recombinant proteins and that is able to carry out post-translational modifications. In this work, Saccharomyces cerevisiae ASNase II gene was expressed in Pichia pastoris (Muts) and was analyzed its localization in extra and intracellular medium and periplasmic space. Also, different conditions of growth and induction were evaluated in shaker, that culminated in a cultivation carried out in 3L bioreactor operated in batch. According the expression analysis, the enzyme was localized in periplasmic space; Pichia pastoris growth in different concentrations of glycerol (10 - 50 g.L-1) show similar kinetic parameters (µmáx = 0.35 h-1; tg = 2.0 h), with the higher substrate to biomass yield (Y x/s= 0.9 g.g-1) obtained with 10 g.L-1 of glycerol. The ASNase expression only occurred at 20°C and improves with methanol concentrations above 1.0% (v/v) to yield the highest production ASNase 3.0% methanol for 48 hours, which was corroborated by factorial fractionated design (3n-k + 2), n = 3 and k = 1. Also was observed that the pH of induction, supplementation with amino acids or casaminoacids and glycerol concentration during the growth phase had little or null influence on the expression. However the induction immediately after glycerol depletion improved the ASNase production. In bioreactor, were used 10 and 40 g.L-1 of glycerol in the growth phase and in the induction phase were used methanol pulses of 3.0% (v/v) during 120 hours. The major volumetric activity (710,2 U.L-1) of ASNase occurred in batch cultivation with 40 g.L-1 of glycerol and periplasmic activity was almost constant during the process which means that the control of pH affects positively the ASNase expression.
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FOLHA, Rodrigo Barbosa. "CR-ASPE: uma técnica de criptografia para dados espaciais armazenados na nuvem." Universidade Federal de Pernambuco, 2017. https://repositorio.ufpe.br/handle/123456789/25851.

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CAPES
Diversas aplicações de banco de dados espaciais e sistemas baseados em localização hospedam-se na nuvem buscando alta disponibilidade e fácil gerenciamento de recursos. Entretanto, os dados armazenados remotamente estão sujeitos à observação dos funcionários da empresa de hospedagem ou das entidades governamentais dos países onde os centros de armazenamento estão localizados. Assim, para manter a confidencialidade dos dados espaciais, este trabalho propôs uma técnica de criptografia para permitir o processamento de busca circulares, poligonais e kNN de dados espaciais criptografados armazenados na nuvem, a técnica CR-ASPE. Baseados na CR-ASPE, dois esquemas de criptografia foram propostos e submetidos a um modelo de ameaça para avaliar o nível de segurança. O primeiro, o CR-ASPE básico, é mais veloz, enquanto o CR-ASPE estendido, ou CRASPEE, mostrou-se mais resistente a ataques. Juntamente, a formalização e a análise do nível de segurança de cada esquema foram apresentadas. Por fim, foi feita uma análise da complexidade temporal das funções do núcleo dos esquemas, assim como uma análise de desempenho de suas funções de busca e de consultas baseadas nestas buscas. No fim deste trabalho, espera-se uma técnica que permita criptografar dados espaciais na nuvem e executar buscas sobre eles com um desempenho superior ao de outras abordagens de criptografia estudadas.
Spatial databases and location-based applications are hosted on the cloud looking for high availability and easy configuration managing. However, the remotely stored data are under foreign governments’ laws and shares resources with other users. Thus, to keep the confidentiality of spatial data, this work proposed a cryptography technique, named CR-ASPE, to enable searches over encrypted spatial data kept in the cloud. Based on CR-ASPE, two cryptography schemes were proposed and submitted to a threat model to evaluate their security level. The first scheme, named CR-ASPE, is faster, on the other hand, the second scheme, named as CR-ASPEE, is more resistant to attacks. A formal definition is shown for each scheme, together with a security analysis. Lastly, time complexity analysis and performance analyses were made to evaluate the functions of each scheme and the queries on a database based on the searches functions. At the end of this work, a technique to encrypt spatial data and run circular, polygonal and kNN searches over them faster than other studied cryptography techniques may be available.
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