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Banson, A., S. O. Ajeighe, and M. A. Ajayi. "Studies on the Control of Mycotoxin Producing Fungi Isolated from Sorghum Sold in Bida, Niger State Nigeria." Journal of Applied Sciences and Environmental Management 27, no. 7 (2023): 1403–7. http://dx.doi.org/10.4314/jasem.v27i7.10.
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Sorghum is an important crop in Africa including Nigeria, Mali and Niger. Fungi contaminate grains including sorghum with fungal poisonous secondary metabolites called mycotoxins. The objectives of this study are the isolation of fungi associated with sorghum in storage and assay for the presence of mycotoxins in stored sorghum. Data obtained showed that stored sorghum used in this study contains Rhizopus stolonifer, Aspergilus oryzae, Aspergilus flavus, Aspergilus niger, Aspergilus solani, Aspergilus terreus and Fusarium oxysposum. Rhizopus stolonifer and Fusarium oxysporum produced zearalenone while Aspergillus oryzae, Aspergillus niger, Aspergillus solani and Aspergillus tereus produced aflatoxins B1. Fumanisin B1 and aflatoxinB1 were produced by Aspergillus flavus. Alium sativum and Zingiber officinale exhibited antifungal activity against the test fungi. This research work will provide a long term economic impact in reducing mycotoxicoses which are acute and chronic toxic diseases caused by mycotoxins. The findings will also serve the purpose of alerting consumers on the dangers of consuming poorly stored sorghum.
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Rohmi, Rohmi, Zainal Fikri, and Ni Ketut Riska Pujasari. "Ubi Jalar Putih (Ipomoea Batatas L.) Media Alternatif Pertumbuhan Aspergillus Niger." Jurnal Kesehatan Prima 13, no. 2 (2019): 143. http://dx.doi.org/10.32807/jkp.v13i2.234.
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Jamur Aspergilus niger menghasilkan alergan yang menyebabkan reaksi alergi, yaitu dapat menyebabkan reaksi hipersensitivitas seperti asma dan alveolitis pada manusia. Ubi jalar putih (Ipomoea batatas L.) mengandung karbohidrat yang dapat digunakan sebagai pengganti sumber karbohidrat pada media PDA. Tujuan penelitian ini adalah untuk mengetahui pengaruh Penggunaan ubi jalar putih (Ipomoea batatas L.) sebagai media alternatif pertumbuhan jamur Aspergillus niger. Penelitian ini bersifat true eksperimet dengan menggunakan 6 replikasi dan 4 perlakuan yaitu media PDA sebagai kontrol, media tepung ubi jalar putih dengan konsentrasi 10%, 20%, dan 30%. Hasil uji laboratorium pada media PDA pertumbuhan diameter jamur Aspergilus niger adalah 43.5 mm dengan sporulasi lebat dan miselium tebal, pada media alternatif tepung ubi jalar putih (Ipomoea batatas L.) pada konsentrasi 10% memiliki pertumbuhan diameter jamur Aspergilus niger adalah 40.8 mm dengan sporulasi tipis dan miselium putih tipis, pada media alternatif tepung ubi jalar putih (Ipomoea batatas L.) pada konsentrasi 20% memiliki pertumbuhan diameter jamur Aspergilus niger adalah 57 mm dengan sporulasi cukup lebat dan miselium putih tipis, dan pada media alternatif tepung ubi jalar putih (Ipomoea batatas L.) pada konsentrasi 30% memiliki pertumbuhan diameter jamur Aspergilus niger adalah 37.5 mm dengan sporulasi cukup lebat dan miselium putih tipis. Dapat disimpulkan bahwa tepung ubi jalar putih (Ipomoea batatas L.) dapat digunakan sebagai media alternatif untuk pertumbuhan Aspergillus niger.
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Nisak, Rohmatin, Farida Fathul, Erwanto Erwanto, and Liman Liman. "PENGARUH LAMA FERMENTASI DAUN NANAS DAN Aspergillus niger TERHADAP KECERNAAN BAHAN ORGANIK DAN SERAT KASAR SECARA IN VITRO." Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) 7, no. 4 (2023): 488–95. http://dx.doi.org/10.23960/jrip.2023.7.4.488-495.
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Penelitian ini bertujuan untuk mengetahui pengaruh dan kombinasi terbaik antara lama fermentasi dan level pemberian Aspergillus niger pada daun nanas terhadap kecernaan bahan organik dan serat kasar secara in vitro. Penelitian ini telah dilaksanakan pada Januari 2022-Maret 2022 bertempat di Laboratorium Nutrisi dan Makanan Ternak dan Laboratorium Ilmu Nutrisi Ternak Perah, Fakultas Peternakan, Institut Pertanian Bogor. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktorial yang terdiri dari 3x3 perlakuan dan 3 ulangan sehingga terdapat 27 unit satuan percobaan. Perlakuan yang digunakan yaitu D0L0 (level Aspergillus niger 0% tanpa difermentasi), D0L1 (level Aspergillus niger 0% dengan lama fermentasi 6 hari), D0L2 (level Aspergillus niger 0% dengan lama fermentasi 12 hari), D1L0 (level Aspergillus niger 2% tanpa difermentasi), D1L1 (level Aspergillus niger 2% dengan lama fermentasi 6 hari), D1L2 (level Aspergillus niger 2% dengan lama fermentasi 12 hari), D2L0 (level Aspergillus niger 4% tanpa difermentasi), D2L1 (level Aspergillus niger 4% dengan lama fermentasi 6 hari, dan D2L2 (level Aspergillus niger 4% dengan lama fermentasi 12 hari). Data yang diperoleh dianalisis ragam pada taraf nyata 5% dan atau 1% dan dilanjutkan menggunakan uji BNT (Beda Nyata Terkecil). Hasil penelitian terdapat interaksi yang berbeda nyata antara lama fermentasi dan level pemberian Aspergillus niger terhadap Kecernaan Bahan Organik dan Serat Kasar. Kombinasi perlakuan terbaik yaitu pada perlakuan D2L0 (level Aspergillus niger 4% tanpa fermentasi) terhadap Kecernaan Bahan Organik sebesar 55,02% dan perlakuan D0L2 (level Aspergillus niger 0% + fermentasi 12 hari) terhadap Kecernaan Serat Kasar sebesar 66,39%.
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Irmawati, Irmawati, Muhtarudin Muhtarudin, Rudy Sutrisna, and Farida Fathul. "PENGARUH LAMA FERMENTASI DAUN NANAS MENGGUNAKAN Aspergillus niger DENGAN LEVEL BERBEDA TERHADAP KONSENTRASI VFA DAN NH3 SECARA IN VITRO." Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) 7, no. 4 (2023): 505–13. http://dx.doi.org/10.23960/jrip.2023.7.4.505-513.
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Penelitian ini bertujuan untuk mengetahui pengaruh terbaik antara lama fermentasi dan level pemberian Aspergillus niger pada daun nanas terhadap konsentrasi VFA dan NH3 secara In Vitro. Penelitian ini dilaksanakan pada Januari-Maret 2022 bertempat di Laboratorium Ilmu Nutrisi Ternak Perah, Fakultas Peternakan, Institut Pertanian Bogor. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktorial yang terdiri dari 3x3 perlakuan dan 3 ulangan sehingga terdapat 27 unit satuan percobaan. Perlakuan yang digunakan yaitu D0L0 (level Aspergillus niger 0% dengan lama fermentasi 0 hari), D0L1 (level Aspergillus niger 0% dengan lama fermentasi 6 hari), D0L2 (level Aspergillus niger 0% dengan lama fermentasi 12 hari), D1L0 (level Aspergillus niger 2% dengan lama fermentasi 0 hari), D1L1 (level Aspergillus niger 2% dengan lama fermentasi 6 hari), D1L2 (level Aspergillus niger 2% dengan lama fermentasi 12 hari), D2L0 (level Aspergillus niger 4% dengan lama fermentasi 0 hari), D2L1 (level Aspergillus niger 4% dengan lama fermentasi 6 hari) dan D2L2 (level Aspergillus niger 4% dengan lama fermentasi 12 hari). Data yang diperoleh dianalisis ragam pada taraf nyata 5% dan atau 1% dan dilanjutkan menggunakan uji BNT. Hasil penelitian terdapat interaksi yang berbeda sangat nyata antara lama fermentasi dan level pemberian Aspergillus niger terhadap konsentrasi VFA dan NH3. Dari hasil penelitian dapat disimpulkan bahwa kombinasi pengaruh terbaik level Aspergillus niger 4% dengan lama fermentasi 0 hari terhadap konsentrasi VFA sebesar 121,73 mM dan kombinasi level Aspergillus niger 0%, 2%, 4% dengan lama fermentasi 0 hari pada konsentrasi NH3 sebesar 10.55, 10.65 dan 10.80 mM.
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KATAYAMA, Kan, Tsuyoshi NAKAYAMA, Sukenari KOYABU, Tomoyasu TAGAMI, Shinsuke NOMURA, and Takeshi NAKANO. "Pulmonary Aspergillus niger." Internal Medicine 42, no. 9 (2003): 912. http://dx.doi.org/10.2169/internalmedicine.42.912.
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Wuryanti, Wuryanti. "Pengaruh Penambahan Biotin Pada Media Pertumbuhan Terhadap Produksi Sel Aspergillus niger." Bioma : Berkala Ilmiah Biologi 10, no. 2 (2012): 46. http://dx.doi.org/10.14710/bioma.10.2.46-50.
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Aspergillus niger is one of microorganisms which has potensial as L-asparaginase source. Biotin are vitamins soluble in water and have function to facilitate the increasing cell growth. The research has purposes to determine the influences of adding biotin into growing medium of Aspergillus niger to its cell production. The results from research show that the optimum incubation time for producing biomass weight of Aspergillus niger was at the 48th hour. Addition of biotin 0.1 mg/L into growing medium of Aspergillus niger might increase biomass weight of Aspergillus niger until 40.17 %.
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Muwakhid, Badat, Umi Kalsum, and Rifa'i Rifa'i. "Kualitas Jerami Jagung (Zea mays) yang di Fermentasi Dengan Aspergillus niger Sebagai Pakan Ternak." Jurnal Nutrisi Ternak Tropis 6, no. 2 (2023): 98–103. http://dx.doi.org/10.21776/ub.jnt.2023.006.02.4.
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Peningkatan kualitas pakan berbahan baku jerami jagung dapat dilakukan dengan pengolahan menggunakan teknologi fermentasi menggunakan kapang. Penelitian ini bertujuan untuk mengetahui kualitas jerami jagung yang telah diberikan perlakuan fermentasi dengan menggunakan Aspergillus niger. Materi pada penelitian ini menggunakan jerami jagung dan Aspergillus niger. Percobaan dalam penelitian ini dilakukan menggunakan metode rancangan acak lengkap. Berdasarkan perlakuan yang diberikan yaitu P0 (kontrol), P1 (Aspergillus niger 105 CFU per gram bahan segar), P2 (Aspergillus niger 106 CFU per gram bahan segar) dan P3 (Aspergillus niger 107 CFU per gram bahan segar). Hasil penelitian mengindikasikan bahwa perlakuan pemberian Aspergillus niger memberikan pengaruh yang signifikan secara nyata (P<0,01) terhadap kadar BO, SK, NDF, ADF, selulosa, BETN, serta kecernaan BK dan BO, sedangkan perlakuan tersebut memberikan pengaruh nyata (P<0,05) terhadap kandungan BK jerami jagung. Berdasarkan penelitian ini, jumlah penambahan Aspergillus niger 107 per gram bahan segar dalam fermentasi jerami jagung memberikan hasil yang paling baik terhadap kualitas pakan yang dihasilkan.
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Mazhar, Modasarah. "GAMMA RAYS MUTAGENESIS OF ASPERGILLUS NIGER FOR HYPERPRODUCTION OF MUTAROTASE." Canadian Journal of Applied Sciences 2 (2012): 173. http://dx.doi.org/10.21065/19257430.173.2.
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Aspergillus niger was subjected to gamma rays mutagenesis at different dose rates (40 to 120 k.Rads). The mutant strains of Aspergillus niger were isolated and selected by random screening method. The selected mutant derived strains were compared with parent type of Aspergillus niger for enhanced production potential of mutarotase. A mutant strain of Aspergillus niger with maximum production potential of mutarotase was finally selected.
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Saurabh, Patil, Sufiyan Mohammed, Anwer Zahid, and Aman B. Upaganlawar Dr. "Pharmacological & Anti-fungal Activity of Garlic (Allium Sativum)." International Journal of Innovative Science and Research Technology 7, no. 11 (2022): 1724–28. https://doi.org/10.5281/zenodo.7480890.
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Due to the growing problem of antifungal resistance as well as the scarcity of antifungals, the prevalence of fungal diseases is rising. As with some fungi that have a negative impact on human health, like Candida albicans, or their useful plants, like Aspergillus niger. Aspergillus niger as well as Candida albicans, two fungi that have been reported, were examined in the current investigation using crushed garlic cloves from of the Sudanese variety. This finding demonstrates that garlic juice has an audible high activity against fungus like Candida albicans and Aspergillus nigar. We have now established the potent antifungal properties of garlic juice. We established that garlic has significant therapeutic properties.
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Ngo, Nha Minh, and Thu Thi Thanh Dong. "STUDYING THE EXTRACTION AND THE IMMOBILIZATION GLUCOAMYLASE FROM ASPERGILLUS NIGER AND ASPERGILLUS AWAMORI ON KAOLIN." Science and Technology Development Journal 12, no. 4 (2009): 61–67. http://dx.doi.org/10.32508/stdj.v12i4.2233.
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Nowadays the immobilized enzyme used universally in industrials thanked to capability re-use and industrial process control capability by enzyme. This research studying the receiving and the immobilization glucoamylase from Aspergillus niger and Aspergillus awamori on kaolin. Activity of enzyme glucoamylase extract from Aspergillus niger is 143325, 62 UI/ g-product enzyme and Aspergillus awamori 133418, 20 UI/ g-enzyme product. Examination the time of support-enzyme, activity of enzyme glucoamylase extract from Aspergillus niger is highest at 50 and from Aspergillus awamori is 40 minutes. The suitable mass of kaolin is 1g/0,1g enzymes The capability re-use of the immobilized enzyme glucoamylase from Aspergillus niger is higher than the immobilized enzyme from Aspergillus awamori.
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Hernández, Mario Cruz, Juan Carlos Contreras Esquivel, Faustino Lara, Raúl Rodríguez, and Cristóbal N. Aguilar. "Isolation and Evaluation of Tannin-degrading Fungal Strains from the Mexican Desert." Zeitschrift für Naturforschung C 60, no. 11-12 (2005): 844–48. http://dx.doi.org/10.1515/znc-2005-11-1205.
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Eleven fungal strains (4 Penicillium commune, 2 Aspergillus niger, 2 Aspergillus rugulosa, Aspergillus terricola, Aspergillus ornatus and Aspergillus fumigatus) were isolated, characterized morphologically and by their capacity to degrade tannins. Aspergillus niger Aa-20 was used as control strain. Several concentrations of hydrolysable tannin (tannic acid) were used as sole carbon source. All strains were able to degrade hydrolysable tannins. Aspergillus niger GH1 and PSH showed the highest tannin-degrading capacity (67 and 70%, respectively). Also, the fungal capacity to degrade condensed tannin (catechin) was tested. Aspergillus niger PSH and Penicillium commune EH2 degraded 79.33% and 76.35% of catechin. The results demonstrated the capacity of fungi to use hydrolysable and condensed tannins as carbon source.
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Rachmawati, Laili, and Mustafidah Udkhiyati. "Toxicity Test of Chromium and Glutaraldehyde to Determine Greener Chemical in Tannery Industry." Materials Science Forum 901 (July 2017): 160–65. http://dx.doi.org/10.4028/www.scientific.net/msf.901.160.
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The aim of this study was to investigate the toxicity level of chromium and glutaraldehyde. This research was conducted by testing the resistance level of fungi (Aspergillus niger sp.) toward glutaraldehyde and chromium in the medium of Potato Dextrose Agar (PDA). Variable of this experiment was the growth of Aspergillus niger sp. that indicates the resistance level of Aspergillus niger sp. The growth rate of Aspergillus niger sp. was classified on four scales. They were, (I) scale 1: zero growth; (II) scale 2: low growth (+); (III) scale 3: medium growth (++); (IV) Scale: 4 high growth (+++). All collected data were analysed by One Way ANOVA. The result showed that the toxicity of chromium and glutaraldehyde tanning material were highly significantly different toward Aspergillus niger (P<0,01). The Aspergillus niger sp. growth rate scale in chromium medium was high (scale 4: 4.00±0.00). Meanwhile, the growth rate scale of the Aspergillus niger sp. in glutaraldehyde medium was low (scale 2: 1.33 ± 0.58). Based on the fungi growth rate, it can be concluded that glutaraldehyde was more toxic than chromium tanning material toward Aspergillus niger sp. Furthermore, it can be said that glutaraldehyde is not quite ideal to be used as an alternative of chromium as a tanning material.
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Karim, Majid, Rashida Perveen, and Syed A. H. Naqvi. "FUNGICIDAL MANAGEMENT OF ASSOCIATED MYCOFLORA WITH STORED SEEDS OF WHEAT AND CHICKPEA." Pakistan Journal of Phytopathology 30, no. 2 (2018): 135. http://dx.doi.org/10.33866/phytopathol.030.02.0426.
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Wheat and chickpea are considered the most important sources of food and energy and stored for longer period of time in the rural areas in the farmers sheds as seed for the next crop and as market commodity to sale. Both of these crops are liable to many mycoflora which not only deteriorate their quality but also leads toward the crop failure during the coming season. A number of seed borne pathogens viz., Aspergills niger, Aspergillus flavus, Aspergillus sulphureus, Aspergillus nidulense, Rhizopus stolonifer, Penicillium spp. and Drechslera spp, were isolated from wheat seed samples while Aspergillus niger, Aspergillus flavus, Bipolaris spp., Penicillium spp. and Rhizopus stolonifer were isolated from seed samples of chickpea. Various broad spectrum fungicides were evaluated against the isolated mycoflora which showed significant result. Score, Topsin-M and ridomil gold to be the most significant fungicides at all doses against the isolated mycoflora.
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Padli, Nadia, Eva Yulia, and Ramaiyulis. "Kadar gula reduksi, protein kasar, dan bahan organik biokonversi kotoran ayam petelur menggunakan kapang Aspergillus niger dan Trichoderma viride sebagai bahan pakan alternatif." Agrivet : Jurnal Ilmu-Ilmu Pertanian dan Peternakan (Journal of Agricultural Sciences and Veteriner) 12, no. 2 (2024): 268–75. https://doi.org/10.31949/agrivet.v12i2.10478.
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Layer manure is generally only used as organic fertilizer, whereas it has the potential as animal feed through bioconversion using Aspergillus niger and Trichoderma viride fungi. The study aims to determine the levels of reducing sugar, crude protein, and organic matter from layer manure resulting from bioconversion with Aspergillus niger and Trichoderma viride. The study used a completely randomized design (CRD) of 4 treatments and four replications. The treatments given were manure inoculated (3% DM) with A: Trichoderma viride (6 days incubation); B Aspergillus niger (6 days incubation); C. Trichoderma viride (3 days incubation) followed by Aspergillus niger (3 days incubation); D. Aspergillus niger (3 days incubation) followed by Trichoderma viride (3 days incubation). The results showed that inoculation of Aspergillus niger and Trichoderma viride in manure bioconversion had a very significant effect (P<0.01) on the levels of reducing sugar, crude protein, and organic matter. The highest levels of reducing sugar were produced in treatment B with Aspergillus niger inoculation. The highest levels of crude protein were produced in treatment A with Trichoderma viride inoculation, and the highest organic matter content was produced through inoculation of a combination of both types of molds. It can be concluded that the manure bioconversion inoculated with Aspergillus niger and Trichoderma viride produced varying levels of reducing sugar, crude protein, and organic matter.
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Yang, Lei, Rui Feng Yang, Jie Lu, and Yan Jun Liu. "Research to Deal with the Pulping Black Liquor by Using Aspergillus niger." Advanced Materials Research 610-613 (December 2012): 2192–96. http://dx.doi.org/10.4028/www.scientific.net/amr.610-613.2192.
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We treat black liquor using Aspergillus niger to extract lignin and to improve the utilization value of pulping black liquor. Results show that: The original black liquor directly to the biological treatment by Aspergillus niger, biological bacteria cannot grow, when reaction liquid pH ≤ 9; Inorganic salt concentration ≤7% (mass ratio); The black liquor load ratio is 20% (mass ratio), the condition of Aspergillus niger growth is good, the ability to produce acid is strong. After treatment of Aspergillus niger, liquid pH decreased to 3, Aspergillus niger changes settling performance of lignin, and it’s good for extraction and purification of lignin. Lignin methoxyl content reduced 2.81%, alcoholic hydroxyl content reduced 0.31%, and carboxyl content increased 0.15%.
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Anwar, Yelfi, Andrianopsyah Mas Jaya Putra, Elvina Dhiaul Iftitah, Partomuan Simanjuntak, and Shirly Kumala. "Prediction of Geraniol Bond Mode in Aspergillus niger Linalool Dehydratase – Isomerase." Majalah Obat Tradisional 24, no. 2 (2019): 133. http://dx.doi.org/10.22146/mot.45599.
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Geraniol is a very valuable aroma chemical and has commonly been used in fragrances and aroma compound. Geraniol biotransformation by Aspergillus niger has been studied. The main bioconversion products obtained from geraniol and liquid culture of A. niger are linalool and alpha-terpineol. Linalool plays a major role in anti-inflammatory, antibacterial and antioxidant activities. This study aims to know the interaction of geraniol in Aspergillus niger enzyme with docking molecular. Comparative modeling of Aspergillus niger enzyme was conducted by means of one of the crystal structure of Linalool Dehydratase – Idomerase (LDI) as a template. The best model of this comparative modeling was then used in docking molecular to investigate geraniol binding mode inactive site enzyme of Aspergillus niger. Inactive site enzyme of Aspergillus niger, geraniol is located with hydrophobic and hydrogen bonds: Amino acid – the amino acids are Asn 105, Arg 96, Lys 112 inactive site - OH with hydrogen bond, Arg 97 inactive site – CH3 with hydrophobic bond and Leu54 inactive site – CH3 with the hydrophobic bond. The distances among pharmacophore respectively are 3,603 A, 6,768 A, and 7,345A. It has higher score (ΔGbind: -3.4 kcal/mol) compared to linalool (ΔGbind: -3.6 kcal/mol). Virtual tethering of linalool with LDI Aspergillus niger enzyme in amino acid Leu120 and Glu118 had been done. The pharmacophore is - OH and methyl C8 group. The distances among pharmacophore respectively are 5,835 Å, 2,52 Å, and 5,32 Å. Virtual tethering of LDI Aspergillus niger enzyme with geraniol has a higher score (ΔGbind: -3.4 kcal/mol) compared to linalool (ΔGbind: -3.6 kcal/mol). It shows that interaction between linalool and LDI Aspergillus niger enzyme is easier to occur than the interaction between geraniol and LDI Aspergillus niger enzyme, geraniol reaction to linalool that occurs is rearrangement reaction.
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Prastio, Hendro, Liman Liman, Rudy Sutrisna, and Muhtarudin Muhtarudin. "PENGARUH BERBAGAI CAMPURAN DAUN SINGKONG DAN ONGGOK TERFERMENTASI ASPERGILLUS NIGER TERHADAP KUALITAS BAHAN KERING, SERAT KASAR, DAN PROTEIN KASAR." Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) 6, no. 2 (2022): 207–13. http://dx.doi.org/10.23960/jrip.2022.6.2.207-213.
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This study aimed to determine the effect of various mixture of cassava leaves and cassava waste fermented by Aspergillus niger on dry matter, crude fiber, and crude protein. This research was carried out in August 2021--September 2021 at the Animal Nutrition and Feeding Laboratory, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung. The experimental design used was a completely randomized design (CRD) with 5 treatments and 3 replications. The treatments were cassava waste without mixture + Aspergillus niger 1% BK (P0), cassava leaves without mixture + Aspergillus niger 1% BK (P1), cassava waste 20% + cassava leaves 80% + Aspergillus niger 1% BK (P2), cassava waste 40% + cassava leaves 60% + Aspergillus niger 1% BK (P3), and cassava waste 60% + 40% cassava leaves + Aspergillus niger 1% BK (P4). The variables observed in this study included dry matter, crude fiber, and crude protein of product of fermentation. The data obtained were analyzed using analysis of variance with a significance level of 1% or 5% and continued with Duncan's Multiple Range Test. The results showed that fermentation with Aspergillus niger 1% BK could reduce the crude fiber content in a mixture of cassava waste and cassava leaves from 18.24% to 12.45% after fermentation, and could increase the crude protein content of fermented products. While the best response of fermentation of mixture of cassava waste and cassava leaves on the chemical quality of dry matter, crude fiber, and crude protein was obtained in a mixture of 40% cassava waste + 60% cassava leaves + Aspergillus niger 1% BK which produced 20.48% crude protein with low crude fiber of 10.81%.
 
 Keywords: Aspergillus niger, crude fiber, crude protein, Dry matter, fermentation
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Nelwida, Nelwida. "Pengaruh Pemberian Kulit Ari Biji Kedelai Hasil Fermentasi dengan Aspergillus niger dalam Ransum terhadap Bobot Karkas Ayam Pedaging." Jurnal Ilmiah Ilmu-Ilmu Peternakan 14, no. 1 (2011): 23–29. http://dx.doi.org/10.22437/jiiip.v0i0.584.
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The study was conducted to determine the effect of feeding fermented epidermis of soybean sedd with Aspergillus niger as substitution of maize and soybean meal in the ration on carcass weight of broiler . The study was carried out in Laboratory of Feed Science to analyze the nutrient content of feedstuffs and in the Cages under Laboratory of Poulty Nutrition, Faculty of Animal Science, University of Jambi for feeding trial. The experiment was designed to Completely Randomized Design (CRD) with 5 treatments and 4 replications. Feeding trial used 100 DOC starins MB-202 P fed basal ration with different level of fermented epidermis of soybean seed with Aspergillus niger as treatment. The treatments were R0 (basal ration without fermented epidermis of soybean seed with Aspergillus niger ), R1 (basal ration containing 10 % of fermented epidermis of soybean seed with Aspergillus niger), R2 (basal ration containing 20 % of fermented epidermis soybean seed with Aspergillus niger), R3 (basal ration containing 30 % of fermented epidermis soybean seed with Aspergillus niger) and R4 (basal ration containing 40 % of fermented epidermis soybean seed with Aspergillus niger). Parameters measured were feed consumption, body weight and carcass weight. Result of this study showed that substitution of maize and soybean meal with fermented epidermis of soybean seed using Aspergillus niger significantly (P<0,05) influenced feed consumption, body weight and absolute carcass weight but no significantlt (P>0,05) on percentage carcass weight of broiler. It is concluded that fermented epidermis of soybean seed with Aspergillus niger could be mixed in to the ration up to 40 % to substitute maize and soybean meal and it was not negative influenced on carcass weight of broiler.
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Susana, I. W. R., B. Tangenjaya, and S. Hastiono. "Selection of phytase-producing moulds." Jurnal Ilmu Ternak dan Veteriner 5, no. 2 (2014): 113–18. https://doi.org/10.14334/jitv.v5i2.207.
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Organic phosphorous in the form of phytate salts are found in feed component originated from cereals which can not be digested by chicken unless after the addition of an enzyme, i.e. phytase. A research to investigate phytase-producing moulds had been carried out. A total of 60 isolates from various collection units and isolation from samples had been collected then screened in Czapek’s medium with Ca-phytate as the source of phosphorous. From mould growth measurements and clearing zones formation there were 4 mould isolates, i.e. Aspergillus ficuum NRRL 3135 and NRRL 320, Aspergillus niger M94/18/D2 (Balitvet isolate), and Aspergillus niger no. 58 (IPB isolate), which were further observed. Enzyme was produced from medium containing 8% of corn’s starch, 3% of glucose, 0.0005% of KCl and MgSO4.7H2O, 0.0002% of K2HPO4, and 0.00018% of FeSO4.7H2O. The enzyme activity, protein and biomass contents were observed at 16, 24, 32, 48 and 72 hours incubations for Aspergillus niger and at 1, 2, 3, 4 and 5 days incubations for Aspergillus ficuum. The highest protein content was found at 5 days incubation for Aspergillus ficuum NRRL 3135 and NRRL 320, (165 and 169 μg/ml respectively), while for Aspergillus niger (Balitvet and IPB isolates) at 16 hours incubation (102 and 112 μg/ml respectively). Meanwhile, the biomass contents were decreased in accordance with the duration of incubations. The maximum enzyme productions were reached at 24 hours incubation for Aspergillus niger and at 4 days incubation for Aspergillus ficuum. The highest phytase activities were reached at 2.808 U/ml for Aspergillus ficuum NRRL 3135, followed by 1.520 U/ml for Aspergillus ficuum NRRL 320. Aspergillus niger produced enzyme quicker than the other moulds, with lower activity level and decreases quicker at the following hours.  Key words: Phytate, phytase, Aspergillus ficuum, Aspergillus niger
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Madika, Abubakar, Ulem Asagha Eugene, Musa Bishir, Mamunu Abdulkadir Sulaiman, and Ibrahim Mohammed Hussaini. "SCREENING OF ASPERGILLUS NIGER ISOLATED FROM SOIL FOR PECTINASE PRODUCTION." FUDMA JOURNAL OF SCIENCES 4, no. 2 (2020): 244–49. http://dx.doi.org/10.33003/fjs-2020-0402-165.
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This study was conducted to screen for pectinase production by Aspergillus niger isolated from soil samples collected from three different locations within Ahmadu Bello University, Zaria, namely; botanical garden, refuse dump and sheep pen sites. A total of fifteen (15) soil samples were collected from different locations and used for isolation by a cultural method. Isolates suspected to be Aspergillus niger were further identified by microscopic examination of the lactophenol cotton blue stained-preparation and slide culture technique. The isolates were then screened in a pectin-containing medium for their pectinase activity. The isolates were further subjected to pectinase production using citrus pectin as the substrate under submerged fermentation conditions. Seven (7) isolates were confirmed to be Aspergillus niger with percentage occurrence of 60% each from sheep pen (SP) and refuse dumpsites (RD), and 20% from the botanical garden (BG). Aspergillus niger RD3 produced the highest zone of pectin hydrolysis (53 ± 1.1 mm) while isolate RD5 produced the lowest (35 ± 3.1 mm). Under submerged fermentation conditions, Aspergillus niger SP5 had the highest pectinase activity of 2.92 U/mL while isolate RD4 had the lowest pectinase activity of 1.29 U/mL. Aspergillus niger can be readily isolated from various soil types with the highest frequency in soils from sheep pen and refuse dumpsites. All the Aspergillus niger isolates demonstrated the potential for pectinase production. The study reveals the potential of various Aspergillus niger isolates from different soil in the production of pectinase.
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Mulyana, Nana, Tri Retno Dyah Larasati, Nurhasni Nurhasni, and Meliana Ningrum. "Peningkatan Aktivitas Enzim Selulase dan Produksi Glukosa Melalui Fermentasi Substrat Jerami Padi Dengan Fungi Aspergillus niger yang Dipapari Sinar Gamma." Jurnal Ilmiah Aplikasi Isotop dan Radiasi 11, no. 1 (2016): 13. http://dx.doi.org/10.17146/jair.2015.11.1.2695.
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Penelitian ini bertujuan untuk meningkatkan aktivitas enzim selulase dan produksi glukosa dalam substrat jerami padi dengan fungi Aspergillus niger yang dipapari sinar gamma Chamber 4000A. Kaldu kentang dektrosa (PDB), garam mineral dengan substrat jerami padi 0 dan 5% berat/volum digunakan sebagai medium cair. Fungi Aspergillus niger dalam media agar miring (slent) dipapari dengan iradiasi gamma pada dosis 0 (kontrol),125, 250, 375, 500 dan 625 Gy. Fungi Aspergillus niger yang dipapari sinar gamma 500 Gy memiliki aktivitas selulase lebih tinggi (2,5 kali) dibanding kontrol (0 Gy) yaitu 2,02 U/ml-2,28 U/ml untuk fungi yang dipapari iradiasi gamma dan 0,60 U/ml-1,12 U/ml untuk kontrol. Pada fermentasi fase padat substrat jerami padi dengan kadar kelembaban awal 81% selama 14 hari menggunakan fungi Aspergillus niger yang dipapari sinar gamma 500 Gy dan kontrol. Hasilnya menunjukkan bahwa fungi Aspergillus niger 500 Gy memiliki aktivitas selulase lebih tinggi (3,9 kali) dibandingkan kontrol yaitu 31,01 U/g untuk fungi yang dipapari sinar gamma dan 7,85 U/g untuk kontrol. Di samping itu, fungi Aspergillus niger (500 Gy) mampu memproduksi glukosa lebih tinggi (2,6 kali) yaitu 125,79 mg/g sedangkan kontrol (0 Gy) adalah 48,00 mg/g. Penggunaan ekstrak enzim kasar yang dihasilkan oleh fungi Aspergillus niger yang dipapar sinar gamma 500 Gy sesuai untuk hidrolisis substrat jerami padi dalam memproduksi glukosa serta mampu meningkatkan aktivitas selulase. Kata kunci : Aspergillus niger, iradiasi gamma, aktivitas selulase, glukosa, fermentasi padat
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Mohamed, Dina Yousif. "Detection the antifungal effect of zirconium oxide nanoparticles on mold which isolated from domestic's bathroom." Al-Mustansiriyah Journal of Science 29, no. 1 (2018): 15. http://dx.doi.org/10.23851/mjs.v29i1.274.
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The aim of this study is to detection the antifungal effect of Zirconium oxide nanoparticles on mold which isolated from domestic's bathroom during April 2017 in Baghdad City. Twenty species were isolated from one hundred samples which were Aspergillus niger, Aspergillus. flavus, , Aspergillus duricaulis, Aspergillus nidulans Aspergillus. parasiticus ,Aspergillus fumigatus, Aspergillus. brasiliensis ,Aspergillus heteromorphus , Curvularia lunata, Penicillium sp., Fusarium oxysporum, , Alternaria alternate, Cladosporium sp. Trichoderma sp., Mucor, Rhizopus sp., Rhodotorula mucilaginosa, , Stachybotrys and yeast. Among the isolated species Aspergillus niger, was the most abundant (14.92%) followed by Aspergillus flavus (10.14%), while less abundant (0.95 & 0.63 %) Cladosporium sp. &, Mucor respectively. The higher demoralization of fungal growth was recorded at 8mg/ml concentration of Zirconium oxide nanoparticles which was (3.8cm) in molds Aspergillus niger, Aspergillus. flavus, , & Aspergillus. brasiliensis
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Nusantara, Mada Jochim, Rudy Sutrisna, Muhtarudin Muhtarudin, and Liman Liman. "PENGARUH CAMPURAN DAUN SINGKONG ONGGOK FERMENTASI MENGGUNAKAN Aspergillus niger TERHADAP BAHAN KERING, ABU, BAHAN ORGANIK, SERAT KASAR, DAN PROTEIN KASAR." Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) 6, no. 4 (2022): 418–29. http://dx.doi.org/10.23960/jrip.2022.6.4.418-429.
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Penelitian ini bertujuan untuk mengetahui pengaruh campuran daun singkong onggok fermentasi menggunakan Aspergillus niger terhadap kualitas bahan kering, abu, bahan organik, serat kasar, dan protein kasar. Penelitian ini dilaksanakan pada Desember 2021--Februari 2022 di Laboratorium Nutrisi dan Makanan Ternak, Jurusan Peternakan, Fakultas Pertanian, Universitas Lampung. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 3 ulangan. Perlakuan yang diberikan yaitu onggok 50% + daun singkong 50% tanpa fermentasi (P0), onggok 20% + daun singkong 80% + Aspergillus niger 1% BK (P1), onggok 30% + daun singkong 70% + Aspergillus niger 1% BK (P2), onggok 40% + daun singkong 60% + Aspergillus niger 1% BK (P3), dan onggok 50% + daun singkong 50% + Aspergillus niger 1% BK (P4). Data yang diperoleh dianalisis menggunakan analisis ragam dengan taraf nyata 1% atau 5% dan dilanjutkan uji berganda Duncan. Hasil penelitian didapatkan fermentasi dengan Aspergillus niger 1% BK dapat menurunkan kandungan serat kasar serta dapat menigkatkan kandungan protein kasar campuran onggok dan daun singkong fermentasi. Respon terbaik pada campuran onggok dan daun singkong terhadap kualitas bahan organik, serat kasar, dan protein kasar didapatkan pada campuran onggok 40% + daun singkong 60% + Aspergillus niger 1% BK yang menghasilkan bahan organik sebesar 40,20% dengan rendah serat kasar sebesar 13,39% dan menghasilkan protein kasar sebesar 24,32%.
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Yakin, Engkus Ainul, Ahimsa Kandi Sariri, and Sri Sukaryani. "Pengaruh Penambahan Aspergilllus niger terhadap Kandungan Nutrien pada Proses Fermentasi Kulit Buah Kakao (Theobroma cacao)." Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) 10, no. 2 (2020): 135. http://dx.doi.org/10.46549/jipvet.v10i2.109.
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Abstract
 This research goal is to determine the nutrient content increased and decreased lignin content of cocoa pod fermented by Aspergillus niger. The assessment method was performed using the three treatments and four replications. T0 = cocoa pod fermentation without the addition of Aspergillus niger, T1 = cocoa pod fermentation by the addition of 5% Aspergillus niger, and T2 = cocoa pod fermentation by the addition of 10% of Aspergillus niger. Fresh cocoa pods were chopped to a size of 1-2 cm. Some of the cocoa pods are directly chopped, rolled, and dried while the rest are supplemented with Aspergillus niger. The mixture is stored within an aerobic medium for seven days. Observed variables include dry matter (DM), crude protein (CP), crude fiber (CFt), crude fat (CF), and lignin. Data were analyzed with analysis of variance unidirectional pattern (Oneway ANOVA) of the completely randomized design (completely randomized design), then if there are significant differences will be further tested by Duncan multiple range test. The results showed that cocoa pod fermented with A. niger decrease of dry matter, crude fat, crude fiber, and lignin. The conclusion is the addition of Aspergillus niger 5% will lower the lignin content (5.38%) compared to without the addition of Aspergillus niger (7.84%).
 Keywords: Aspergillus niger; Cocoa pod; Fermentation; Lignin
 
 Abstrak
 Penelitian dilakukan untuk mengetahui peningkatan kandungan nutrien dan penurunan kandungan lignin dari kulit buah kakao (KBK) yang difermentasi dengan A. niger. Metode penelitian yang dilakukan yaitu menggunakan tiga perlakuan dan empat ulangan. P0 = KBK fermentasi tanpa penambahan A. niger, P1 = KBK fermentasi dengan penambahan 5% A. niger, dan P2=KBK fermentasi dengan penambahan 10% A. niger . KBK segar dicacah dengan ukuran 1-2 cm. Sebagian KBK yang dicacah langsung dikeringkan kemudian digiling dan sebagian lainnya diberi penambahan A. niger. Campuran dimasukkan ke dalam wadah aerob selama 7 hari. Penelitian ini dirancang menggunakan desain penelitian rancangan acak lengkap. Variabel yang diamati meliputi kandungan bahan kering (BK), protein kasar (PK), serat kasar (SK), lemak kasar (LK) dan lignin. Data dianalisis menggunakan sidik ragam pola searah (oneway ANOVA) dan dilanjutkan uji jarak berganda Duncan (Duncan multiple range test/DMRT), apabila nilai P<0,05. Hasil penelitian menunjukkan bahwa fermentasi kulit buah kakao dengan A. niger menurunkan kandungan air, lemak kasar, serat kasar dan lignin. Disimpulkan bahwa penambahan A. niger 5% dapat menurunkan kandungan lignin (5,38%) dibandingkan dengan tanpa penambahan A. niger 10% (7,84%).
 Kata kunci : Aspergillus niger; Fermentasi; Kulit buah kakao; Lignin
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Ajibo, Adeyinka Elizabeth, and Rukayat Olaitan Said. "Production of gluconic acid from sweet potato peels using naturally occurring fungi by submerged fermentation." Bio-Research 21, no. 1 (2023): 1828–37. http://dx.doi.org/10.4314/br.v21i1.6.
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The oxidation of glucose produces gluconic acid, a significant organic acid. The aim of this study was to produce gluconic acid from sweet potato peels by submerged fermentation. Isolation and identification of fungi were done using standard microbiological methods. Proximate analysis of substrate and screening of fungal isolates for gluconic acid production was done using standard procedures. Gluconic acid yields were determined using High Performance Liquid Chromatography. A standard gluconic acid producer, Aspergillus niger ATCC 10577, was used as control. A sum of six different fungal species were isolated and identified. They included Aspergillus niger, Aspergillus flavus, Penicillum sp., Cladosporium sp., Rhizopus stolonifer and Aspergillus terreus. Proximate composition of the sweet potato peels showed percentage carbohydrate of 20.81 ± 0.07, percentage moisture of 64.02 ± 0.27. Screening for gluconic acid production showed that Aspergillus niger had the highest zone of clearance and identified as Aspergillus niger UFMGCB 14248. Our data further showed that gluconic acid concentrations (mg/ml) was highest at substrate concentration 50 g/L, carbon source starch, incubation day 7 and pH 6 for both Aspergillus niger UFMGCB 14248 and Aspergillus niger ATCC 10577. The findings showed that the fungal isolates used in this study were good gluconic acid producers
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Alyaa Hussein Talib and Ihsan Flayyih Hasan AL-Jawhari. "Decolorization and Degradation of azo dyes in textile wastewater effluent by Aspergillus Niger and Aspergillus ochraceous." GSC Advanced Research and Reviews 17, no. 3 (2023): 109–21. http://dx.doi.org/10.30574/gscarr.2023.17.3.0473.
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In this study, fungi were isolated from contaminated soil collected from textile wool factories in Thi Qar Governorate. The results showed a high potential for removing Methylene Blue dye by Aspergillus niger and Aspergillus ochraceous. The removal process was conducted under optimized conditions, including different concentrations of the azo dye, pH levels, carbon sources, and nitrogen sources. The results indicated that the fungi Aspergillus niger and Aspergillus. Ochraceus were the most common isolates. Both fungi were treated with Methylene Blue dye in solid and liquid media, and both showed the ability to degrade this dye. However, Aspergillus niger demonstrated higher activity in color removal compared to Aspergillus ochraceous, with a diameter of (9) cm in solid medium for Aspergillus niger and (8) cm for Aspergillus. ochraceus. These results were confirmed by statistical analysis, which showed significant differences between these fungi and concentrations. The decolorization percentage was confirmed by Fourier Transform Infrared (FTIR) spectroscopy of which showed complete disappearance of peaks at 500 nm and at 1500 nm, indicates the degradation of dyes due to fungal activity, The technology also confirmed that a pH of 7 was the optimal pH for dye biodegradation compared to pH 4. This was observed through the dry weight of the fungi, with Aspergillus niger reaching to (2.35) and Aspergillus ochraceous reaching to (2.15) at pH 7. Also, in pH 4, the dry weight of Aspergillus niger and Aspergillus. ochraceus reached (2.17) and (2.05) respectively. Furthermore, the study confirmed the potential use of filamentous fungi in treating dye-contaminated water, regardless of their pH values.
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Marpaung, Agustina E., T. Sabrina, Abdul Rauf, and Dwi N. Susilowati. "The role of phosphate solubilizing fungi from the shallot rhizosphere on increasing the growth and yield of shallot on Andisol." IOP Conference Series: Earth and Environmental Science 1417, no. 1 (2024): 012003. https://doi.org/10.1088/1755-1315/1417/1/012003.
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Abstract Plants need the nutrient P to increase growth and yield. P availability is low in Andisol soil and has high retention by 97.8%. Giving the phosphate solubilizing fungi (PSF) is an alternative to increase available P which plants can use to increase growth and yield. This research aims to determine the benefits of providing phosphate-solubilising fungi on the growth and yield of shallots in Andisol. The study used a block randomized design with three replications. The first factor is Andisol soil origin (Karo regency and Tapanuli Utara regency) and the second is isolate of phosphate solubilizing fungi (Aspergillus niger P13, Aspergillus niger P21, Aspergillus pseudodeflectus BJ21, and Aspergillus niger BJ23). The research results showed that the soil from Karo can increase plant dry weight and root dry weight respectively by 36.38% and 37.66% compared to Tapanuli Utara. The application of Aspergillus pseudodeflectus BJ21 and Aspergillus niger BJ23 fungi was able to increase plant dry weight by 35.10% and 34.25% and root dry weight by 29.38% and 23.46% compared to without PSF application. The soil origin of Karo Regency increased yield by 36.40% from Tapanuli Utara Regency. Giving fungi Aspergillus pseudodeflectus BJ21 and Aspergillus niger BJ23 fungi was able to increase plant dry weight by 35.10% and 34.25% and root dry weight Aspergillus niger P13 was able to increase yield of 65.63% and 63.12% compared to without giving PSF.
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Makwin Danladi Makut, Chioma Deborah Nzeduru, Ike Kenneth Ekeleme, Jibril Egwu Owuna, and Obuneme Smart Obiekezeie. "Production of gluconic acid by fungal species isolated from soil in Keffi, Nigeria." World Journal of Advanced Research and Reviews 11, no. 3 (2021): 382–90. http://dx.doi.org/10.30574/wjarr.2021.11.3.0465.
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This investigation aimed at production of gluconic acid by fungal species isolated from soil in Keffi. Standard microbiological methods were employed for isolation and identification of the fungal isolates. The yields of gluconic acid produced by the different isolates of the fungi were determined using gas chromatograph and mass Spectrometry. The occurrence of fungi showed that Rhizopus oryzae was 100%, Aspergillus carneus was 75.0%, Aspergillus niger was 75.0% and Aspergillus terreus was 100% while Trichoderma viride was 25.0% and Fusarium moniliforme was 25.0%. The result further demonstrated that three species of the fungal isolates Aspergillus niger, Aspergillus carneus and Fusarium moniliforme were found to produce gluconic acid. Screening for gluconic acid production showed that Aspergillus carneus isolated from locations As1, Cs1 and Cs2, Aspergillus niger isolated from locations Bs1, Bs2 and Cs4 were able to produce gluconic acid. Result of effect of temperature, pH, substrate concentration and fermentation time on production of gluconic acid showed that Aspergillus niger Bs2 produced highest amount of gluconic acid at 28oC, similarly highest amount for gluconic acid produced by Aspergillus carneus As1 was at 28oC, whereas Fusarium moniliforme Bs4 produced highest at 30OC. pH 6.5 was found to the best optima pH for production of both gluconic acid for the fungi studied namely Aspergillus niger Bs2 and Aspergillus carneus As1 and Fusarium moniliforme Bs4 produced highest gluconic acid at pH 5.5. The substrate concentration showed highest production of gluconic acid was produced by Aspergillus niger Bs2 at substrate concentration of 25%. Aspergillus carneus As1 produced highest at substrate concentration of 20% and Fusarium moniliforme Bs4 produced highest at substrate concentration of 20%. The fermentation time showed highest production of gluconic acid by Aspergillus niger Bs2 and Aspergillus carneus As1 was after 144 hours whereas F. moniliforme Bs4 produced gluconic acid after 120hrs respectively. The fungi species isolated from soil in keffi revealed great ability in production of gluconic acid.
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Dewi, Pramesti, Retno Indrati, Ria Millati, and Sardjono Sardjono. "Effect of Lime Pretreatment on Microstructure of Cassava Stalk Fibers and Growth of Aspergillus niger." Biosaintifika: Journal of Biology & Biology Education 10, no. 1 (2018): 205–12. http://dx.doi.org/10.15294/biosaintifika.v10i1.13802.
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Cassava stalk can be converted into sugar-based product by using microorganism. Unfortunately, lignin act as a barrier of optimal bioconversion. Cassava stalk needs pretreatment process for removing this barrier. The effect of lime pretreatment on microstructure of cassava stalk fibers and the growth of Aspergillus niger FNCC 6114 were observed in this research. The cassava stalks were reduced into 0.147- 0.297 mm size and pretreated with 1 % Ca(OH)2. Lime pretreated and unpretreated cassava stalk was used as solid medium for Aspegillus niger FNCC 6114. The effect of pretreatment method on fibers microstructure of cassava stalk was evaluated through SEM micrograph. The growth and metabolism activities of Aspergillus niger FNCC 6114 were monitored through SEM micrograph of media after fermentation. The other parameters examined were changes in glucosamine, reducing sugar levels, and spores’ quantity. Lime pretreatment altered microstructure of cassava stalk fibers. However, cassava stalk without lime pretreatment gave better growth of Aspergillus niger FNCC 6144 based on metabolism activities parameters. Cassava stalks is suitable as media for Aspergillus niger FNCC 6144 through solid state fermentation. For better growth of Aspergillus niger FNCC 6144 fine-sized cassava stalk should not be lime pretreated. The results of this study provide information about the pretreatment of cassava stems which was effective in supporting the growth of Aspergillus niger. Enhancements the utilization of cassava stems by using fungi, for example Aspergillus niger can overcome the accumulation of organic waste that can interfere with environmental sustainability.
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De la Motte, Rebecca S., and Fred W. Wagner. "Aspergillus niger sulfhydryl oxidase." Biochemistry 26, no. 23 (1987): 7363–71. http://dx.doi.org/10.1021/bi00397a025.
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Elenwo, C. E., S. O. N. Dimkpa, and G. C. Amadi. "Molecular and Morphological Assessment of the Impact of Gas Flares on the Fungal Abundance and Diversity in soils of Niger Delta Area, Nigeria." British Journal of Environmental Sciences 12, no. 6 (2024): 48–59. http://dx.doi.org/10.37745/bjes.2013/vol12n64859.
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Fungal abundance and diversity in soils of three communities flow stations (ID, IR and OB) owned by an unnamed oil exploration firm operating in the Niger Delta area for about five decades were determined using nucleic acid analytical methods. Top soil (0-15 cm) samples were collected from mini pits using sterile bottles at 50, 100 and 150 meters from the flare barrier of each community flow station and soil samples from the control was taken at 2.5 kilometers during the dry and wet seasons. The fungal properties were determined first using the cultural method of serial dilution method which revealed the presence of the following fungi; Aspergillus sp, and penicillium chrysogenum at 50 m away from flare in all the three flow stations during the dry seasons and in the wet season Aspergillus sp, penicillium chrysogenum, Mucor sp., yeast cells, A. niger, and Rhizopus sp. At 100 m away from flare, Mucor sp, A. niger and rhizopus were isolated in all three-flow station in the dry season and in the wet season, Yeast cells, A. niger, Aspegillus sp. and Rhizopus were isolated. P. chrysogenum, Aspergillus sp. and yeast cells were isolated at 150 m away from flare in the dry season and in the same distance, yeast cells, A. niger aggregate, A. niger and P. chrysogenum were isolated. At the control site, during the dry season, A. niger, P. chrysogenum, Aspergillus sp., Rhizopus sp. and Botryodiplodia threobome were isolated in the dry and A. niger, P. chrysogenum and yeast in the wet season. Fungal DNA was extracted using Norgen fungal genomic DNA extraction kits for the molecular analysis to establish the true characteristics and species identity of the isolates earlier identified with cultural methods. This revealed the presence of the following fungi upon sequence blast on NCBI database; Aspergillus fumigatus (Aspergillus fumigatus brown 2 (abr2) gene, complete cds strain), Aspergillus fumigatus (Aspergillus fumigates strain IHM 15988 pigment biosynthesis protein), Fusarium phyllophilum , Fusarium graminearum, Aspergillus oryzae (a strain of Aspergillus niger) Aspergillus oryzae (niger). Findings from this work have shown that molecular technique gave the exact identity of fungal species isolated from study area. All six (6) molecularly identified fungi were isolated from the three-study flow station and in both seasons. The Aspergillus group was dominant as a result of their ability to adapt to the flare environment
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Adebayo, M. R., and T. O. Osuolale. "Biopreservative Potential of Lemon Grass (Cymbopogon citratus) Oil against Common Food Spoilage Fungi." Journal of Applied Sciences and Environmental Management 28, no. 7 (2024): 2049–56. http://dx.doi.org/10.4314/jasem.v28i7.16.
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Food spoilage fungi are becoming a global challenge to food storage and food safety, hence tons of food are lost annually to the activities of these fungi while consumption of spoilt food are dangerous to human health. Therefore, the objective of this paper is to assess the antifungal activities of lemon grass oil (Cymbopogon citratus) against some food spoilage fungi using different standard techniques such as dilution plating technique, colonial and morphological characterization. Oil extraction was done using fractional distillation method and biocompounds evaluation with GC-MS, antifungal activity, the minimum inhibitory (MI) and fungicidal concentrations (MFC) were determine using standard methods. Results show that five fungi were identified as Aspergillus niger, Penicillum sp., Aspergillus oryzae, Aspergillus flavusand Aspergillus terreus. The GC-MS of the oil detectscitral, citronellol, neral, geranial, limonene and geranyl acetate, flavonoids, alcohol and terpenoids. The oil was cidal to 100 % of the fungi at stock concentration. The minimum inhibitory concentrations against Aspergillus niger, Aspergillus oryzae and Aspergillus terreus was 0.781 μL/ml, Penicillumsp. 0.391μL/ml, and Aspergillus flavus, 6.25μL/ml. The MFC for Aspergillus nigerwas 1.563μL/ml, 0.781μL/ml for Penicillumsp and Aspergillus oryzae 6.25μL/ml for Aspergillus flavus and 1.563 μL/ml for Aspergillust erreus. Lemon grass oil extracted was active against common food spoilage fungi examined and could be a potential biopreservative and antifungal agent.
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Miłek, Justyna. "Thermodynamics and kinetics of thermal deactivation of catalase Aspergillus niger." Polish Journal of Chemical Technology 22, no. 2 (2020): 67–72. http://dx.doi.org/10.2478/pjct-2020-0018.
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AbstractThe thermal stability of enzyme-based biosensors is crucial in economic feasibility. In this study, thermal deactivation profiles of catalase Aspergillus niger were obtained at different temperatures in the range of 35°C to 70°C. It has been shown that the thermal deactivation of catalase Aspergillus niger follows the first-order model. The half-life time t1/2 of catalase Aspergillus niger at pH 7.0 and the temperature of 35°C and 70°C were 197 h and 1.3 h respectively. Additionally, t1/2 of catalase Aspergillus niger at the temperature of 5°C was calculated 58 months. Thermodynamic parameters the change in enthalpy ΔH*, the change in entropy ΔS* and the change Gibbs free energy ΔG* for the deactivation of catalase at different temperatures in the range of 35°C to 70°C were estimated. Catalase Aspergillus niger is predisposed to be used in biosensors by thermodynamics parameters obtained.
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Zaharia, Roxana, Cristina Petrișor, Petruța Cornea, Camelia Diguță, Stelica Cristea, and Ștefan Sorin. "Isolation and Molecular Identification of Fungal Isolates from Stored Cereals Using PCR-RFLP Method." Romanian Agricultural Research 39 (2022): 13–22. http://dx.doi.org/10.59665/rar3902.
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Contamination of grain cereals with toxic metabolites of fungi, both pathogenic and saprotrophic, is one of the particularly important problems in global agriculture. The aim of the current study was molecular identification of fungi isolates from different samples of stored cereals and evaluate the utility of PCR-RFLP of the ITS region technique. The results established that the most abundant species were found belonging to Aspergillus genera (50%), followed by Fusarium spp. (19%) and Penicillium spp. (19%). Aspergillus flavus was the most frequent species, representing almost 40% of the isolates belonging to the genus Aspergillus. Also were identified as Aspergilus versicolor, Aspergilus ruber and Aspergilus niger by molecular analysis representing 10% each.
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Nasiu, Firman, Wa Laili Salido, Andi Murlina Tasse, Syamsuddin Syamsuddin, Hairil A. Hadini, and Amiluddin Indi. "Evaluasi Kecernaan In Vitro Bahan Kering dan Bahan Organik Kulit Singkong Fermentasi Sebagai Bahan Pakan Ternak." Jurnal Ilmu dan Teknologi Peternakan Tropis 7, no. 2 (2020): 127. http://dx.doi.org/10.33772/jitro.v7i2.11482.
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ABSTRAKPenelitian ini bertujuan untuk melakukan evaluasi kecernaan in vitro bahan kering dan bahan organik kulit singkong yang difermentasi menggunakan jamur Aspergillus niger dan EM-4 sehingga dapat memberikan informasi tambahan mengenai kualitas kulit siongkong yang difermentasi maupun tanpa fermentasi. Rancangan penelitian yang digunakan adalah rancangan acak lengkap (RAL) yang terdiri atas 4 perlakuan dan 4 ulangan. Perlakuan yang diberikan terhadap kulit singkong adalah P1 (tanpa fermentasi), P2 (fermentasi dengan Aspergillus niger), P3 (fermentasi dengan EM-4), P4 (fermentasi dengan kombinasi A. niger dan EM-4). Evaluasi kecernaan in vitro ini dilakukan dengan menggunakan metode Tilley and Terry. Hasil penelitian menunjukkan bahwa penambahan jamur A. niger dan EM-4 dalam proses fermentasi kulit singkong dapat meningkatkan kecernaan bahan kering dan bahan kering kulit singkong secara signifikan dimana hasil terbaik diperoleh pada penambahan kombinas A. niger dan EM-4.Kata kunci: Aspergillus niger, bahan kering, bahan organik, EM-4, kecernaan in vitroABSTRACTThe aim of this study was to evaluate the in vitro digestibility of dry matter and organic matter of fermented cassava peel using Aspergillus niger and EM-4 inoculant to provide additional information about the quality of fermented and unfermented cassava peel. The study design used was a completely randomized design (CRD) consisting of 4 treatments and 4 replications. The treatments were P1 (without fermentation), P2 (fermented with Aspergillus niger), P3 (fermented with EM-4), P4 (fermented with a combination of A. niger and EM-4). This in vitro digestibility evaluation was conducted by using the Tilley and Terry method. The results showed that the addition of A. niger fungi and EM-4 in the fermentation process of cassava peel could significantly improve the digestibility of dry matter and dry matter of cassava peel where the best results were obtained on the addition of A. niger and EM-4 combinations.Keywords: Aspergillus niger, dry matter, EM-4, in vitro digestibility, organic matter
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Zhan, Shou Fa, Ye Chen, and De Si Sun. "Effects of Potassium-Dissolution Microorganisms on Surface Properties of Orthoclase and Bioleaching." Advanced Materials Research 753-755 (August 2013): 109–13. http://dx.doi.org/10.4028/www.scientific.net/amr.753-755.109.
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The alternation of surface properties of orthoclase after biological conditioning with Bacillus mucilaginosus and Aspergillus niger was investigated by zeta potential and adsorption studies. Bioleaching potassium from orthoclase by the two strains were also studied. Results show that IEPs of the two strain continuously decrease in their each logarithmic phase and stable phase. the IEP of orthoclase, after which interaction with cells, moves towords the IEP of cells. The adsorption of B·mucilaginosus is faster and higher than Aspergillus niger onto orthoclase. The potassium extractions by adapted B·mucilaginosus and unadapted B·mucilaginosus reach 24.0% and 18.5% respectively, 23.0% and 20.0% respectively by adapted Aspergillus niger and unadapted Aspergillus niger after bioleaching 9 d.
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Alyaa, Hussein Talib, and Flayyih Hasan AL-Jawhari Ihsan. "Decolorization and Degradation of azo dyes in textile wastewater effluent by Aspergillus Niger and Aspergillus ochraceous." GSC Advanced Research and Reviews 17, no. 3 (2023): 109–21. https://doi.org/10.5281/zenodo.11212488.
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In this study, fungi were isolated from contaminated soil collected from textile wool factories in Thi Qar Governorate. The results showed a high potential for removing Methylene Blue dye by <em>Aspergillus niger</em> and <em>Aspergillus ochraceous</em>. The removal process was conducted under optimized conditions, including different concentrations of the azo dye, pH levels, carbon sources, and nitrogen sources. The results indicated that the fungi <em>Aspergillus niger </em>and <em>Aspergillus. Ochraceus</em> were the most common isolates. Both fungi were treated with Methylene Blue dye in solid and liquid media, and both showed the ability to degrade this dye. However, <em>Aspergillus niger</em> demonstrated higher activity in color removal compared to Aspergillus<em> ochraceous</em>, with a diameter of (9) cm in solid medium for <em>Aspergillus niger</em> and (8) cm for <em>Aspergillus. ochraceus</em>. These results were confirmed by statistical analysis, which showed significant differences between these fungi and concentrations. The decolorization percentage was confirmed by Fourier Transform Infrared (FTIR) spectroscopy of which showed complete disappearance of peaks at 500 nm and at 1500 nm, indicates the degradation of dyes due to fungal activity, The technology also confirmed that a pH of 7 was the optimal pH for dye biodegradation compared to pH 4. This was observed through the dry weight of the fungi, with <em>Aspergillus niger</em> reaching to (2.35) and <em>Aspergillus ochraceous</em> reaching to (2.15) at pH 7. Also, in pH 4, the dry weight of <em>Aspergillus niger</em> and <em>Aspergillus. ochraceus</em> reached (2.17) and (2.05) respectively. Furthermore, the study confirmed the potential use of filamentous fungi in treating dye-contaminated water, regardless of their pH values.
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Ramaiyulis, Ramaiyulis, Muhammad Fajar, and Salvia Salvia. "Inokulasi Aspergillus Niger, Trichoderma Viride, dan Saccharomyces Cerevisiae pada Manure Layer dalam Produksi Protein Sel Tunggal untuk Pakan Ternak." Jurnal Peternakan Lokal 6, no. 2 (2024): 80–88. http://dx.doi.org/10.46918/peternakan.v6i2.2309.
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Protein sel tunggal merupakan salah satu bahan sumber protein yang berasal dari mikroba dan dapat digunakan untuk menggantikan bahan pakan protein yang mahal. Penelitian ini bertujuan untuk mengetahui inokulasi Aspergillus niger, Trichoderma viride, dan Saccharomyces cerevisiae pada manure layer dalam produksi protein sel tunggal untuk pakan ternak. Metode penelitian ini menggunakan desain Rancangan Acak Lengkap (RAL) dengan 4 perlakuandan 4 ulangan. Perlakuan A (Trichoderma viride + Saccharomyces cerevisiae), B (Aspergillus niger + Saccharomyces cerevisiae), C ( Trichoderma viride + Aspergillus niger + Saccharomyces cerevisiae ), D (Aspergillus niger + Trichoderma viride + Saccharomyces cerevisiae). Parameter yang diamati dalam penelitian ini adalah kandungan bahan kering, kandungan protein kasar, dan produksi protein sel tunggal. Hasil penelitian ini menunjukkan persentase kandungan bahan kering berkisar 44,43% - 61,77%. Persentase kandungan protein kasar berkisar 17,33 – 21,68%. Produksi protein sel tunggal berkisar 754 (mg/ml) – 1.608 (mg/ml). Berdasarkan hasil penelitian disimpulkan inokulasi Aspergillus niger, Trichoderma viride, dan Saccharomyces cerevisiae pada manure layer berpengaruh nyata (P<0,05) terhadap kandungan bahan kering, kandungan protein kasar, dan produksi protein sel tunggal.
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Kusters-van Someren, Margo A., Jan A. M. Harmsen, Harry C. M. Kester, and Jaap Visser. "Structure of the Aspergillus niger pelA gene and its expression in Aspergillus niger and Aspergillus nidulans." Current Genetics 20, no. 4 (1991): 293–99. http://dx.doi.org/10.1007/bf00318518.
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Yani, Sri Sukar, Muhamad Arif Fadillah, Catur Suci Purwati, Engkus Ainul Yakin, and Muhammad Husein. "Nutritional quality of fermented bran using aspergillus niger with different incubation time." Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) 14, no. 4 (2024): 165–69. https://doi.org/10.46549/jipvet.v14i4.415.
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This study aims to determine the effect of long fermentation of rice bran using Aspergillus niger fungus on the content of dry matter (BK), crude protein (PK), and crude fiber (SK). This study used a unidirectional pattern Complete Randomized Design (RAL) with four treatments, and each treatment was repeated three times, namely T0: Fermentation of bran using 2.5% Aspergillus niger for 0 days. Q1: Fermentation of bran using 2.5% Aspergillus niger for 3 days. Q2: Fermentation of bran using 2.5% Aspergillus niger for 5 days. Q3: Fermentation of bran using 2.5% Aspergillus niger for 7 days. Fermentation of rice bran with the addition of 5 grams of urea and 20 ml of molasses The results showed that the average value of BK obtained T0: 94.43%, T1: 82.53%, T2: 95.41%, and T3: 88.01%. The average PK value was obtained: T0:13.03%, T1:13.27%, T2:14.12%, and T3:15.27%. The average SK value was obtained: T0:16.64%, T1:16.56%, T2:15.78%, and T3:12.76%. The conclusion obtained from this study is that the fermentation period of 0 to 7 days using Aspergillus niger mushrooms as much as 2.5% has a real effect on dry matter and crude fiber but has no real effect on crude protein content.
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Kadek Sinta Cahyani, Ni Diah, Ida Bagus Rai Wiadnya, Ari Khusuma, and I. Wayan Getas. "Analisis Konsentrasi Letal Dan Waktu Letal Isolat Jamur Aspergillus Niger Terhadap Nyamuk Aedes Aegypti." Jurnal Analis Medika Biosains (JAMBS) 9, no. 2 (2022): 78. http://dx.doi.org/10.32807/jambs.v9i2.264.
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Latar Belakang: Nyamuk Aedes aegypti merupakan penyebab utama penyakit DBD, kasus DBD di dunia pada tahun 2015 sebanyak 3,2 juta kasus. Di Indonesia pada tahun 2018 terdapat 65.602 kasus. Upaya pengendalian terhadap vektor DBD telah banyak dilakukan baik dengan menggunakan insektisida kimia dan alami salah satunya dengan jamur Aspergillus niger sebagai fungi entomopatogen. Tujuan Penelitian: Mengetahui konsentrasi letal dan waktu letal dari isolat jamur Aspergillus niger terhadap nyamuk Aedes aegypti. Metode Penelitian: Penelitian ini merupakan penelitian true eksperimental dengan rancangan acak lengkap. Perlakuan dengan 3 konsentrasi isolat jamur Aspergillus niger yaitu 10-5, 10-6, 10-7 spora/ml dengan 6 replikasi. Penelitian ini menggunakan obat nyamuk semprot sebagai kontrol positif. Data diperoleh dianalisa secara statistik menggunakan analisis probit. Hasil Penelitian: Konsentrasi isolat jamur Aspergillus niger yang efektif terhadap nyamuk Aedes aegypti adalah konsentrasi 10-7. Hasil analisis probit uji patogenitas isolat jamur Aspergillus niger terhadap nyamuk Aedes aegypti dengan tingkat kepercayaan 95% didapatkan LC50, LC70, LC90 adalah 6,1 x 107 spora/ml, 8,5 x 107 spora/ml, 1,1 x 108 spora/ml dan diketahui LT50, LT70, LT90 berturut-turut adalah 1,919 jam, 1,510 jam, 1,184 jam. Kesimpulan: Konsentrasi isolat jamur Aspergillus niger secara signifikan memberikan pengaruh terhadap kematian nyamuk Aedes aegypti.
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Subhabrata, Guha Barman, and K. Banik A. "Development of high concentration hexavalent chromium resistant strain of Aspergillus niger for improved Cr6+ biosorption : Scanning Electron Microscopic and spectrophotometric studies." Journal of Indian Chemical Society Vol. 87, Jul 2010 (2010): 847–55. https://doi.org/10.5281/zenodo.5797251.
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Biotechnology Division, Department of Chemical Engineering, University of Calcutta, 92, Acharya Prafulla Chandra Road, Kolkata-700 009, India <em>E-mail :</em> sg_barman@yahoo.co.in <em>Manuscript received 25 August 2009, accepted 14 January 2010</em> Hexavalent chromium (Cr<sup>6+</sup>) is one of the most potent heavy metal pollutant released from various industries into water bodies. Most commonly used physico-chemical methods, for the removal of Cr<sup>6+</sup> from waste waters are Inefficient, expensive and also produces unmanagable toxic sludge. Recent studies showed that biosorption, based on metal-biomass interaction can be an alternative to commonly employed methods for Cr<sup>6+</sup> removal from waste waters. Among the various biosorbent, bacteria, yeast, algae and fungi hue potential for Cr<sup>6+</sup> biosorption. In this study, <em>Bacillus circulans</em> MTCC 3161, <em>Saccharomyces cenvisiae</em> and <em>Aspergillus niger</em> were screened for high Cr<sup>6+</sup> concentration tolerant strain isolation. It was found that, <em>Bacillus circulans</em> MTCC 3161 and Sacclulromycts cenvisiae are resistant up to 800 ppm of Initial Cr<sup>6+</sup> concentration present In the fermentation media, whereas, <em>Aspergillus niger</em> can resist up to 900 ppm of Initial Cr<sup>6+</sup> concentration. Moreover, It was also found that Cr<sup>6+</sup> resistant <em>Aspergillus niger</em> have better biosorption potential (48% at 800 ppm) than that of Cr<sup>6+</sup> resistant <em>Bacillus circulans</em> MTCC 3161 and <em>Saccharomyces cerevisiae </em>(40% and 38% respectively at 800 ppm). Scanning Electron Microscopic studies of mycelia with spores of parent cells of <em>Aspergillus niger</em>, Cr<sup>6+</sup> resistant <em>Aspergillus niger</em> and Cr<sup>6+</sup> loaded Cr'+ resistant <em>Aspergillus niger</em> revealed that exposure of parent <em>Aspergillus niger</em> to high Cr<sup>6+</sup> concentration induces morphological changes. Moreoler, Cr<sup>6+</sup> loaded Cr<sup>6+</sup> resistant <em>Aspergillus niger</em> shows further changes in mycelial and hyphal morphology which occurs may be due to biosorption of Cr<sup>6+</sup> onto Cr<sup>6+</sup> resistant <em>Aspergillus niger</em>. Metal-biomass Interaction was confirmed by Fourier Transform Infra Red (FTIR) spectroscopic study which also Indicates the presence of hydroxyl, amine, amide, carbonyl or carboxyl groups, on cell wall or <em>Aspergillus niger.</em>
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Salman, Buraq shakir, Hashim M. S, Fadwa abdulrazaq Jameel, et al. "Study about environmental naturally fungi infection in pet animals with experimental study." Journal of Kerbala for Agricultural Sciences 11, no. 2 (2024): 76–93. http://dx.doi.org/10.59658/jkas.v11i2.2066.
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The current study was designed to investigate Environmental and natural fungi infection in pet animals and then experimentally to study fungi effects on tissues. Naturally infectious samples were fifty swabs in different pet animals (dogs, cats, rats, pigeons, and chickens) from the veterinary hospital of Eden Square in January 202. The findings were Cladosporum spp at (16%) ; Aspergillus flavus, Aspergillus niger at (14%); Rhizopus spp at (8%); Aspergillus ochraceus, Aspergillus terreus, and Penicillium chrysogenum at (6%); Chrysosporium spp, and Cryptococcus neoformans at (4%); Aspergillus fumigatus , Aspergillus ustus and Fusarium spp at (2%) in percentage with other unknown fungi. In doge, only 9 (9/50) samples of fungi, mainly Aspergillus flavus at (33.3%) with cladogram spp.at (22.3%). In cats, also 9 (9/50), mainly Penicillium spp at (22.25 %). In wild rats, fifty cotton swabs showed 12 (12/50) mainly Aspergillus niger, besides Rhizopus spp. At (25%). While in pigeons, only 10 (10/50), mainly Aspergillus niger, Cladosporum spp., and Cryptococcus neoformans at (20%). In chicken, only 5 (5/50), mainly Aspergillus ochraceus at (40%). Fifty albino mice aged 6-8 weeks and 25± 3gm body weight. They were separated in plastic cages and kept for adaptation at 2-3 weeks in the Animal House of the College of Veterinary Medicine, and they were fed standard pellets and water. These animals were divided into 5 groups containing 10 mice each: 1st one: control group treated with normal saline; 2nd Group infected with 0.1ml of Aspergillus flatus intraperitoneally; 3rd group infected with Aspergillus ochraceus 0.1ml intraperitoneally (IP); 4th group infected with 0.1ml Aspergillu niger IP; 5th group mice infected with Cryptococcus neoformans 0.1ml IP at single dose. After 3 weeks animals were scarified. Histopathological examination, in general, reports high incidences of necrotic lesions with granuloma and severe destructive changes, mainly in areas containing fungi hyphae that appear very clearly enclosed to necrotic and inflammatory foci, lung tissues suffering from prominent interstitial pneumonia with thick exudation, mainly fibrinous type. Liver tissues show massive necrosis with Kupfer cells and other inflammatory cell proliferation and evidence of apoptosis. Kidney samples appear with heavy degenerative changes ranging from swelling tubules to necrosis associated with severe inflammation caused by pyelonephritis and phlebitis. also, we saw fungi in the affected area. Spleen shows severe depletion and megakaryocyte proliferation and necrotic lesion. Fungi naturally infects occurs in pet animals and can transmitted to other animals in different environmental; area and can causes severe pathological lesions.
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Salmon, Amelia M., Mellissa E. S. Ledo, and Merpiseldin Nitsae. "KARAKTERISASI SUBSTRAT DAN SUHU EKSTRAK KASAR LIPASE Aspergillus niger M1407." Jurnal Saintek Lahan Kering 3, no. 1 (2020): 13–15. http://dx.doi.org/10.32938/slk.v3i1.1038.
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Isolasi ekstrak kasar Aspergillus niger M1407 menggunakan medium tepung biji kesambi melalui solid state fermentation telah dilakukan sehingga perlu dilakukan karakterisasi substrat dan suhu untuk menentukan kondisi optimal reaksi enzim substrat ekstrak kasar lipase Aspergillus niger M1407.Penelitian ini bertujuan untuk mengetahui profil substrat dan suhu ekstrak kasar lipase Aspergillus nigerM1407, Penelitian ini dilakukan di laboratorium (mikrobiologi dan kimia) Program Studi Pendidikan Biologi Universitas Kristen Artha Wacana Kupang, pada bulan januari 2019 – maret 2019. Metode yang digunakan dalam penelitian ini terbagi menjadi empat tahapan yaitu pembuatan medium untuk isolat lipase Aspergillus niger M1407; pembuatan medium Solid-State Fermentation selama 7 hari; karakterisasi substrat menggunakan asam oleat +etanol dalam isooktan dan asam oleat + metanol dalam isooktan dan karakterisasi suhu pada variasi suhu 20oC, 30oC, 40oC, 50oC, dan 60oC; serta pengujian Analisis aktivitas lipase menggunakan metode titrasi. Data yang di peroleh di analisis secara deskriptif kuantitatif. Hasil penelitian ini menunjukan bahwa aktivitas enzim ekstrak kasar lipase Aspergillus niger M1407 lebih baik pada substrat asam oleat dalam metanol yang memiliki aktivitas 200 U/mL sedangkan karakterisasi suhu, merupakan aktivitas ekstrak kasar lipase Aspergillus niger M1407 memiliki suhu optimum pada suhu 40oC dengan aktivitas enzim yaitu 580 u/mL.
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Ma, Li Wei, Jin Song Yang, Li Juan Zhang, and Hai Sheng Tan. "Optimization of Inferior Banana Saccharifiction with Response Surface Methodology." Advanced Materials Research 864-867 (December 2013): 553–57. http://dx.doi.org/10.4028/www.scientific.net/amr.864-867.553.
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A study was implemented, referring to inferior banana saccharification on the glycosylation interaction of α-amylase and Aspergillus niger. During the experiment of defective banana saccharification, the interaction of the additive amount of α-amylase, Aspergillus niger, initial pH, saccharification temperature and saccharification time was analyzed. On the basis of confirmation of solid-to-liquid ratio of inferior bananas, saccharification temperature and additive amount of α-amylase, the interaction of the additive amount of Aspergillus niger, the initial pH and saccharification time in the different response surface was experimented and analyzed, then the optimal proportions of glycosylation saccharifying inferior bananas were as follows: Aspergillus niger 0.5%, saccharification time 7 h, pH 4.61. Brix of reducing sugar is 16.693 mg/g.
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Sun, Ke, Yu-Jie Zhang, Si-Yuan Tong, Meng-Di Tang, and Chang-Bao Wang. "Study on Rice Grain Mildewed Region Recognition Based on Microscopic Computer Vision and YOLO-v5 Model." Foods 11, no. 24 (2022): 4031. http://dx.doi.org/10.3390/foods11244031.
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This study aims to develop a high-speed and nondestructive mildewed rice grain detection method. First, a set of microscopic images of rice grains contaminated by Aspergillus niger, Penicillium citrinum, and Aspergillus cinerea are acquired to serve as samples, and the mildewed regions are marked. Then, three YOLO-v5 models for identifying regions of rice grain with contamination of Aspergillus niger, Penicillium citrinum, and Aspergillus cinerea in microscopic images are established. Finally, the relationship between the proportion of mildewed regions and the total number of colonies is analyzed. The results show that the proposed YOLO-v5 models achieve accuracy levels of 89.26%, 91.15%, and 90.19% when detecting mildewed regions with contamination of Aspergillus niger, Penicillium citrinum, and Aspergillus cinerea in the microscopic images of the verification set. The proportion of the mildewed region area of rice grain with contamination of Aspergillus niger/Penicillium citrinum/Aspergillus cinerea is logarithmically correlated with the logarithm of the total number of colonies (TVC). The corresponding determination coefficients are 0.7466, 0.7587, and 0.8148, respectively. This study provides a reference for future research on high-speed mildewed rice grain detection methods based on MCV technology.
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Silfiyani, Wahyu, Muhtarudin Muhtarudin, Farida Fathul, and Erwanto Erwanto. "PENGARUH LAMA FERMENTASI DAUN NANAS DAN TINGKAT PENGGUNAAN Aspergillus niger TERHADAP KECERNAAN PROTEIN KASAR DAN LEMAK KASAR SECARA IN VITRO." Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) 7, no. 4 (2023): 514–22. http://dx.doi.org/10.23960/jrip.2023.7.4.514-522.
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Penelitian ini bertujuan untuk mengetahui pengaruh lama fermentasi dan level terbaik pemberian Aspergillus niger pada daun nanas terhadap kecernaan protein kasar dan lemak kasar secara in vitro. Penelitian ini dilaksanakan pada bulan Januari--Maret 2022 berlokasi di Laboratorium Makanan Ternak, Jurusan Peternakan, Fakultas Pertanian, Universitas Lampung. Untuk analisis kecernaan protein kasar dan lemak kasar dilaksanakan di Laboratorium Nutrisi Perah, Fakultas Peternakan, Institut Pertanian Bogor. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 3 × 3 perlakuan dan 3 ulangan sehingga terdapat 27 unit satuan percobaan. Perlakuan yang diberikan yaitu lama fermentasi (0, 6, dan 12 hari) dan level Aspergillus niger (0, 2, dan 4%). Data yang diperoleh dianalisis ragam pada taraf nyata 5% dan atau 1% dan dilanjutkan menggunakan uji BNT (Beda Nyata Terkecil). Hasil penelitian terdapat interaksi yang berbeda nyata antara lama fermentasi dan level pemberian Aspergillus niger terhadap kecernaan ptorein kasar dan lemak kasar. Kombinasi perlakuan terbaik yaitu pada perlakuan D1L0 (level Aspergillus niger 2% tanpa fermentasi) terhadap kecernaan protein kasar sebesar 61,33% dan perlakuan D2L0 (level Aspergillus niger 4% tanpa fermentasi) terhadap kecernaan lemak kasar sebesar 72,83%.
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Hashim, Huda Raheem, Wisam Jasim Abed Ali, and Zina Abdulhussein Jawad. "Calculating the Percentage of Air Pollution with Fungi Through Rainwater." Asian Journal of Water, Environment and Pollution 21, no. 3 (2024): 19–25. http://dx.doi.org/10.3233/ajw240030.
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The first location where the frequency ratios of the isolated fungi were 73.78% - Aspergillus niger, 66.87% - Aspergillus flavus, 34.69% - Alternaria sp., 22.99% - Fusarium sp., 15.65% - Rhizopus sp., 11.54% - Penicillium sp. and Cladosprium sp. - 5.87% was in the north of Al-Diwaniya city. Whereas the percentage of fungi frequency in the air before the rains occurred in the same location was 41.77% Aspergillusniger, 43.56% Aspergillusflavus, 11.56% Alternaria sp., 8.95% Fusarium sp., 4.64% Rhizopus sp., 4.55% Penicillium sp. and Cladosprium sp. was 0% (while the frequency ratio for fungi isolated from the air after the rain was 3.45% Aspergillus niger, 2.63% Aspergillus flavus, 0% Alternaria sp., 0% Fusarium sp., 0% Rhizopus sp., 0% Penicillium sp. and Cladosprium sp. was 0%). In the second location in the south of Al-Diwaniya city the frequency ratios of isolated fungi was 77.45% Aspergillus niger, 71.64% Aspergillus flavus, 40.18% Fusarium sp., 30.24% Rhizopus sp., 23.85% Alternaria sp. and Penicillium sp. was 14.74%, respectively, while the fungus Cladosprium sp. did not appear. The results showed that the percentage of fungi contamination was more in the southern region of the city. Whereas the percentage of fungi frequency in the air before the rains occurred in the same location was 50.43% Aspergillus niger, 48.54% Aspergillus flavus, 22.36% Fusarium sp., 18.19% Rhizopus sp., 13.54% Alternaria sp., 11.67% Penicillium sp. and Cladosprium sp. was 0%. While was the frequency ratio for fungi isolated from the air after the rain was 3.46% Aspergillus niger, 3.53% Aspergillus flavus, 0% Alternaria sp., 0% Fusarium sp., 0% Rhizopus sp., 0% Penicillium sp. and Cladosprium sp. was 0%).
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Rida, Siddique* Komal Siddiqui Noor-e.-Saba and Faria Aslam. "SCREENING OF ASPERGILLUS NIGER FOR BIODEGARADATION OF DIFFERENT HYDROCARBONS." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 09 (2018): 9084–90. https://doi.org/10.5281/zenodo.1433697.
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Today hydrocarbons/oil pollution causes serious damage to our environment. Chemical or physical methods are failed to degrade such contaminants hence biodegradation provides simple and cost effective process. In this study the biodegradation potential of Aspergillus niger for different hydrocarbons was analyzed. Two kinds of hydrocarbons i.e. edible (almond oil, mustard oil and cooking oil) and non-edible (engine oil, diesel and petrol) were used. For the initial screening, culture was inoculated in Bushnell-Haas (BH) plate assay each plate containing respective hydrocarbon. Aspergillus niger displayed highest growth on medium containing cooking oil. While least growth was noted on petrol and diesel. Furthermore to analyze the degradation ability of Aspergillus niger, dextrose media was used. The biodegradable efficiency of Aspergillus niger was noted on the basis of dry weight, total protein, total sugar and reducing sugar in presence of hydrocarbons. The highest growth of Aspergillus niger was noted on media containing 2% cooking oil after 6 days of incubation. Among the non-edible hydrocarbons highest growth was noted on 3% engine oil after 6 days of incubation. Total protein content on edible hydrocarbon was found to be higher than non-edible hydrocarbons. Total sugar content showed great variation among edible and non-edible hydrocarbons. Reducing sugar was found to be very low in both edible and non edible hydrocarbon containing media. 2, 6-dichlorophenol indophenols (DCPIP) dye assay was also used for detection. The highest reduction in absorbance was observed on edible hydrocarbons than non-edible hydrocarbons. Hence Aspergillus niger have ability to degrade hydrocarbons at different rate. Key words: Biodegradation, Aspergillus niger, edible hydrocarbons, non edible hydrocarbons.
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Kristandia, Yanuardi, Fanny M. Laihad, and Astrid Palmasari. "Pengaruh Induksi Aspergillus Niger/Brasilliensis Strain ATCC®16404™ Secara Sistemik Dan Tindakan Pencabutan Gigi Terhadap Jumlah Koloni Pada Mukosa Gingiva." DENTA 9, no. 2 (2015): 163. http://dx.doi.org/10.30649/denta.v9i2.18.
Full textAbstract:
<strong>Latar belakang:</strong> Pencegahan kerusakan jaringan mukosa gingiva disebabkan infeksi jamur invasive aspergillus niger dalam mulut masih sulit untuk ditentukan diagnosis dan terapinya. Kerusakan mukosa gingiva maksila penyebabnya dapat di indikasikan karena infeksi jamur sistemik yang dipicu oleh pencabutan gigi. Belum ada penelitian mengenai pengaruh infeksi jamur invasif aspergillus niger pada mukosa maksila yang dilakukan pencabutan dan tidak dilakukan pencabutan. <strong>Tujuan:</strong> Untuk mengetahui pengaruh induksi <em>aspergillus niger/ brasiliensis strain ATCC<sup>®</sup>16404™ </em>secara sistemik dan tindakan pencabutan gigi terhadap jumlah koloni pada mukosa gingiva maxilla.<strong> Bahan dan metode: </strong>Penelitian ini menggunakan rancangan post test only control group design. 32 tikus wistar jantan dewasa dibagi secara acak menjadi 4 kelompok. Kelompok (K-) sweb mukosa maksila, kelompok (P1) pencabutan dan sweb, (P2) sweb dan suntik 0,3 ml jamur <em>aspergillus niger</em> strain ATCC<sup>®</sup>16404™ 0,5 <em>Mc Farland</em>, (P3) sweb, pencabutan 1 gigi maxilla dan suntik 0,3 ml jamur <em>aspergillus niger</em> strain ATCC<sup>®</sup>16404™ 0,5 <em>Mc Farland</em>. Dilakukan sweb tiap kelompok (Hari 1,3,5) di mukosa maxilla dan dilakukan pemeriksaan mikrobiologi kultur media <em>saboround dextrose agar</em> (SDA) dengan tehnik <em>spreader</em> dan diinkubasi (37°C) selama 48 jam dan dilakukan pemeriksaan secara makroskopis dan mikroskopis. Data dianalisis dengan uji statistik Kruskal Wallis. <strong>Hasil :</strong> Tidak ada perbedaan bermakna jumlah jamur aspergillus niger pada setiap kelompok. <strong>Kesimpulan: </strong>Induksi aspergillus niger secara sistemik ternyata belum bisa menyebabkan kondisi yang signifikan pada rongga mulut, maka dari itu pencabutan bukan merupakan faktor yang memicu keparahan timbulnya aspergillus niger