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1

Haladjova, Emi, Stanislav Rangelov, Martin Geisler, et al. "Asymmetric Flow Field-Flow Fractionation Investigation of Magnetopolyplexes." Macromolecular Chemistry and Physics 216, no. 18 (2015): 1862–67. http://dx.doi.org/10.1002/macp.201500177.

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2

Hupfeld, Stefan, Dominik Ausbacher, and Martin Brandl. "Asymmetric flow field-flow fractionation of liposomes: optimization of fractionation variables." Journal of Separation Science 32, no. 9 (2009): 1465–70. http://dx.doi.org/10.1002/jssc.200800626.

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3

Wagner, Michael, Stephan Holzschuh, Anja Traeger, Alfred Fahr, and Ulrich S. Schubert. "Asymmetric Flow Field-Flow Fractionation in the Field of Nanomedicine." Analytical Chemistry 86, no. 11 (2014): 5201–10. http://dx.doi.org/10.1021/ac501664t.

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4

Eskelin, Katri, Mirka Lampi, Florian Meier, Evelin Moldenhauer, Dennis H. Bamford, and Hanna M. Oksanen. "Asymmetric flow field flow fractionation methods for virus purification." Journal of Chromatography A 1469 (October 2016): 108–19. http://dx.doi.org/10.1016/j.chroma.2016.09.055.

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5

Kirkland, J. J., C. H. Dilks, S. W. Rementer, and W. W. Yau. "Asymmetric-channel flow field-flow fractionation with exponential force-field programming." Journal of Chromatography A 593, no. 1-2 (1992): 339–55. http://dx.doi.org/10.1016/0021-9673(92)80303-c.

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6

Pitkänen, Leena, Päivi Tuomainen, and Katri Eskelin. "Analysis of plant ribosomes with asymmetric flow field-flow fractionation." Analytical and Bioanalytical Chemistry 406, no. 6 (2013): 1629–37. http://dx.doi.org/10.1007/s00216-013-7454-4.

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7

Schachermeyer, Samantha, Jonathan Ashby, and Wenwan Zhong. "Aptamer–protein binding detected by asymmetric flow field flow fractionation." Journal of Chromatography A 1295 (June 2013): 107–13. http://dx.doi.org/10.1016/j.chroma.2013.04.063.

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8

Dutz, Silvio, Judith Kuntsche, Dietmar Eberbeck, Robert Müller, and Matthias Zeisberger. "Asymmetric flow field-flow fractionation of superferrimagnetic iron oxide multicore nanoparticles." Nanotechnology 23, no. 35 (2012): 355701. http://dx.doi.org/10.1088/0957-4484/23/35/355701.

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9

Yohannes, G., S. K. Wiedmer, M. Jussila, and M. L. Riekkola. "Fractionation of Humic Substances by Asymmetrical Flow Field-Flow Fractionation." Chromatographia 61, no. 7-8 (2005): 359–64. http://dx.doi.org/10.1365/s10337-005-0510-2.

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10

Runyon, J. Ray, Lars Nilsson, Johan Alftrén, and Björn Bergenståhl. "Characterization of oat proteins and aggregates using asymmetric flow field-flow fractionation." Analytical and Bioanalytical Chemistry 405, no. 21 (2013): 6649–55. http://dx.doi.org/10.1007/s00216-013-7115-7.

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11

Sulaeva, Irina, Philipp Vejdovszky, Ute Henniges, Arnulf Kai Mahler, Thomas Rosenau, and Antje Potthast. "Molar Mass Characterization of Crude Lignosulfonates by Asymmetric Flow Field-Flow Fractionation." ACS Sustainable Chemistry & Engineering 7, no. 1 (2018): 216–23. http://dx.doi.org/10.1021/acssuschemeng.8b02856.

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12

Jochem, Aljosha-Rakim, Genesis Ngwa Ankah, Lars-Arne Meyer, Stephan Elsenberg, Christoph Johann, and Tobias Kraus. "Colloidal Mechanisms of Gold Nanoparticle Loss in Asymmetric Flow Field-Flow Fractionation." Analytical Chemistry 88, no. 20 (2016): 10065–73. http://dx.doi.org/10.1021/acs.analchem.6b02397.

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13

Loeschner, Katrin, Jana Navratilova, Samuel Legros, et al. "Optimization and evaluation of asymmetric flow field-flow fractionation of silver nanoparticles." Journal of Chromatography A 1272 (January 2013): 116–25. http://dx.doi.org/10.1016/j.chroma.2012.11.053.

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14

Hee Moon, Myeong, P. Stephen Williams, Dukjin Kang, and Inmi Hwang. "Field and flow programming in frit-inlet asymmetrical flow field-flow fractionation." Journal of Chromatography A 955, no. 2 (2002): 263–72. http://dx.doi.org/10.1016/s0021-9673(02)00226-1.

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15

Niezabitowska, Edyta, Adam R. Town, Bassem Sabagh, et al. "Insights into the internal structures of nanogels using a versatile asymmetric-flow field-flow fractionation method." Nanoscale Advances 2, no. 10 (2020): 4713–21. http://dx.doi.org/10.1039/d0na00314j.

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16

Itabashi, Daisuke, Reiko Murao, Shunsuke Taniguchi, Kazumi Mizukami, Hideaki Takagi, and Masao Kimura. "Determination of Size Distribution of Nanoparticles Using Asymmetric Flow Field-flow Fractionation (AF4)." ISIJ International 60, no. 5 (2020): 979–87. http://dx.doi.org/10.2355/isijinternational.isijint-2019-387.

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17

Koopmans, G. F., T. Hiemstra, I. C. Regelink, B. Molleman, and R. N. J. Comans. "Asymmetric flow field-flow fractionation of manufactured silver nanoparticles spiked into soil solution." Journal of Chromatography A 1392 (May 2015): 100–109. http://dx.doi.org/10.1016/j.chroma.2015.02.073.

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18

Iavicoli, Patrizia, Patricia Urbán, Angelo Bella, Maxim G. Ryadnov, François Rossi, and Luigi Calzolai. "Application of Asymmetric Flow Field-Flow Fractionation hyphenations for liposome–antimicrobial peptide interaction." Journal of Chromatography A 1422 (November 2015): 260–69. http://dx.doi.org/10.1016/j.chroma.2015.10.029.

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19

Hu, Yingwen, Rachael M. Crist, and Jeffrey D. Clogston. "The utility of asymmetric flow field-flow fractionation for preclinical characterization of nanomedicines." Analytical and Bioanalytical Chemistry 412, no. 2 (2019): 425–38. http://dx.doi.org/10.1007/s00216-019-02252-9.

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20

Galyean, Anne A., James J. Filliben, R. David Holbrook, Wyatt N. Vreeland, and Howard S. Weinberg. "Asymmetric flow field flow fractionation with light scattering detection – an orthogonal sensitivity analysis." Journal of Chromatography A 1473 (November 2016): 122–32. http://dx.doi.org/10.1016/j.chroma.2016.10.063.

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21

Zielke, Claudia, Catalina Fuentes, Lennart Piculell, and Lars Nilsson. "Co-elution phenomena in polymer mixtures studied by asymmetric flow field-flow fractionation." Journal of Chromatography A 1532 (January 2018): 251–56. http://dx.doi.org/10.1016/j.chroma.2017.12.028.

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22

Podzimek, Stepan, and Christoph Johann. "Asymmetric Flow Field-Flow Fractionation: Current Status, Possibilities, Analytical Limitations and Future Trends." Chromatographia 84, no. 6 (2021): 531–34. http://dx.doi.org/10.1007/s10337-021-04035-w.

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23

Kang, Dukjin, and Myeong Hee Moon. "Miniaturization of Frit Inlet Asymmetrical Flow Field-Flow Fractionation." Analytical Chemistry 76, no. 13 (2004): 3851–55. http://dx.doi.org/10.1021/ac0496704.

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24

Fuentes, Catalina, Jaeyeong Choi, Claudia Zielke, J. Mauricio Peñarrieta, Seungho Lee, and Lars Nilsson. "Comparison between conventional and frit-inlet channels in separation of biopolymers by asymmetric flow field-flow fractionation." Analyst 144, no. 15 (2019): 4559–68. http://dx.doi.org/10.1039/c9an00466a.

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Asymmetric flow field-flow fractionation (AF4) is a separation technique in which a focusing/relaxation step is used after the sample is injected onto the separation channel. This step is avoid when a Frit Inlet (FI-AF4) channel is used.
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25

Osorio-Macías, Daniel E., Dongsup Song, Johan Thuvander, et al. "Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation." Journal of Agricultural and Food Chemistry 68, no. 49 (2020): 14564–76. http://dx.doi.org/10.1021/acs.jafc.9b07251.

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26

Yang, Joon Seon, and Myeong Hee Moon. "Flow optimisations with increased channel thickness in asymmetrical flow field-flow fractionation." Journal of Chromatography A 1581-1582 (December 2018): 100–104. http://dx.doi.org/10.1016/j.chroma.2018.10.053.

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27

Itabashi, Daisuke, Reiko Murao, Shunsuke Taniguchi, Kazumi Mizukami, Hideaki Takagi, and Masao Kimura. "Determination of the Size Distribution of Nanoparticles Using Asymmetric Flow Field-flow Fractionation (AF4)." Tetsu-to-Hagane 107, no. 1 (2021): 82–91. http://dx.doi.org/10.2355/tetsutohagane.tetsu-2020-088.

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28

Hupfeld, Stefan, Dominik Ausbacher, and Martin Brandl. "Asymmetric flow field-flow fractionation of liposomes: 2. Concentration detection and adsorptive loss phenomena." Journal of Separation Science 32, no. 20 (2009): 3555–61. http://dx.doi.org/10.1002/jssc.200900292.

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29

Li, Jingjing, Yonggang Liu, and Quan Chen. "Conformation of dilute poly(vinyl alcohol)-borax complex by asymmetric flow field-flow fractionation." Journal of Chromatography A 1624 (August 2020): 461260. http://dx.doi.org/10.1016/j.chroma.2020.461260.

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30

Moon, Myeong Hee, and Inmi Hwang. "HYDRODYNAMIC VS. FOCUSING RELAXATION IN ASYMMETRICAL FLOW FIELD-FLOW FRACTIONATION." Journal of Liquid Chromatography & Related Technologies 24, no. 20 (2001): 3069–83. http://dx.doi.org/10.1081/jlc-100107720.

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31

LIU, Pan-Pan, Can QUAN, Hong-Mei LI, and Jun-Su JIN. "Characterization of Nanoparticle Diameter by Asymmetrical Flow Field-Flow Fractionation." CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION) 41, no. 7 (2013): 1063. http://dx.doi.org/10.3724/sp.j.1096.2013.21224.

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32

Andreev, Victor P., Yuri V. Stepanov, and J. C. Giddings. "Field-flow fractionation with asymmetrical electroosmotic flow. I. Uncharged particles." Journal of Microcolumn Separations 9, no. 3 (1997): 163–68. http://dx.doi.org/10.1002/(sici)1520-667x(1997)9:3<163::aid-mcs4>3.0.co;2-#.

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33

Andreev, V. P., and Y. V. Stepanov. "Field-Flow Fractionation with Asymmetrical Electroosmotic Flow. II. Charged Particles." Journal of Liquid Chromatography & Related Technologies 20, no. 16-17 (1997): 2873–86. http://dx.doi.org/10.1080/10826079708005598.

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34

Oh, Sunok, Dukjin Kang, Sung-Min Ahn, Richard J. Simpson, Bong-Hee Lee, and Myeong Hee Moon. "Miniaturized asymmetrical flow field-flow fractionation: Application to biological vesicles." Journal of Separation Science 30, no. 7 (2007): 1082–87. http://dx.doi.org/10.1002/jssc.200600394.

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35

Quattrini, Federico, Germán Berrecoso, José Crecente-Campo, and María José Alonso. "Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers." Drug Delivery and Translational Research 11, no. 2 (2021): 373–95. http://dx.doi.org/10.1007/s13346-021-00918-5.

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AbstractThe importance of polymeric nanocarriers in the field of drug delivery is ever-increasing, and the accurate characterization of their properties is paramount to understand and predict their behavior. Asymmetric flow field-flow fractionation (AF4) is a fractionation technique that has gained considerable attention for its gentle separation conditions, broad working range, and versatility. AF4 can be hyphenated to a plurality of concentration and size detectors, thus permitting the analysis of the multifunctionality of nanomaterials. Despite this potential, the practical information that
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36

Moon, Myeong Hee, Hansun Kwon, and Ilyong Park. "Stopless Flow Injection in Asymmetrical Flow Field-Flow Fractionation Using a Frit Inlet." Analytical Chemistry 69, no. 7 (1997): 1436–40. http://dx.doi.org/10.1021/ac960897b.

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37

Gigault, Julien, Tae Joon Cho, Robert I. MacCuspie, and Vincent A. Hackley. "Gold nanorod separation and characterization by asymmetric-flow field flow fractionation with UV–Vis detection." Analytical and Bioanalytical Chemistry 405, no. 4 (2012): 1191–202. http://dx.doi.org/10.1007/s00216-012-6547-9.

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38

Gigault, Julien, and Vincent A. Hackley. "Observation of size-independent effects in nanoparticle retention behavior during asymmetric-flow field-flow fractionation." Analytical and Bioanalytical Chemistry 405, no. 19 (2013): 6251–58. http://dx.doi.org/10.1007/s00216-013-7055-2.

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39

Mudalige, Thilak K., Haiou Qu, Germarie Sánchez-Pomales, Patrick N. Sisco, and Sean W. Linder. "Simple Functionalization Strategies for Enhancing Nanoparticle Separation and Recovery with Asymmetric Flow Field Flow Fractionation." Analytical Chemistry 87, no. 3 (2015): 1764–72. http://dx.doi.org/10.1021/ac503683n.

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40

Guan, Xia, Rafael Cueto, Paul Russo, Yadong Qi, and Qinglin Wu. "Asymmetric Flow Field-Flow Fractionation with Multiangle Light Scattering Detection for Characterization of Cellulose Nanocrystals." Biomacromolecules 13, no. 9 (2012): 2671–79. http://dx.doi.org/10.1021/bm300595a.

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41

Lattuada, Marco, Carlos Olivo, Cornelius Gauer, Giuseppe Storti, and Massimo Morbidelli. "Application of Asymmetric Flow-Field Flow Fractionation to the Characterization of Colloidal Dispersions Undergoing Aggregation." Langmuir 26, no. 10 (2010): 7062–71. http://dx.doi.org/10.1021/la904390h.

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42

Zhang, Haiying, Daniela Freitas, Han Sang Kim, et al. "Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation." Nature Cell Biology 20, no. 3 (2018): 332–43. http://dx.doi.org/10.1038/s41556-018-0040-4.

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43

Gigault, Julien, John M. Pettibone, Charlène Schmitt, and Vincent A. Hackley. "Rational strategy for characterization of nanoscale particles by asymmetric-flow field flow fractionation: A tutorial." Analytica Chimica Acta 809 (January 2014): 9–24. http://dx.doi.org/10.1016/j.aca.2013.11.021.

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44

Viktor, Zanelle, and Harald Pasch. "Variable temperature asymmetric flow field-flow fractionation for the topology separation of poly(methyl methacrylate)." Analytica Chimica Acta 1144 (February 2021): 150–57. http://dx.doi.org/10.1016/j.aca.2020.12.017.

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45

Zhang, Haiying, and David Lyden. "Asymmetric-flow field-flow fractionation technology for exomere and small extracellular vesicle separation and characterization." Nature Protocols 14, no. 4 (2019): 1027–53. http://dx.doi.org/10.1038/s41596-019-0126-x.

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46

Abbate, Raffaele Andrea, Norbert Raak, Susanne Boye, et al. "Asymmetric flow field flow fractionation for the investigation of caseins cross-linked by microbial transglutaminase." Food Hydrocolloids 92 (July 2019): 117–24. http://dx.doi.org/10.1016/j.foodhyd.2019.01.043.

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47

Safenkova, Irina V., Elvira S. Slutskaya, Vasily G. Panferov, Anatoly V. Zherdev, and Boris B. Dzantiev. "Complex analysis of concentrated antibody-gold nanoparticle conjugates’ mixtures using asymmetric flow field-flow fractionation." Journal of Chromatography A 1477 (December 2016): 56–63. http://dx.doi.org/10.1016/j.chroma.2016.11.040.

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48

Podzimek, Stepan, and Christoph Johann. "Correction to: Asymmetric Flow Field-Flow Fractionation: Current Status, Possibilities, Analytical Limitations and Future Trends." Chromatographia 84, no. 7 (2021): 703. http://dx.doi.org/10.1007/s10337-021-04054-7.

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49

Liang, Qihui, Di Wu, Bailing Qiu, and Nanyin Han. "Present situation and development trends of asymmetrical flow field-flow fractionation." Chinese Journal of Chromatography 35, no. 9 (2017): 918. http://dx.doi.org/10.3724/sp.j.1123.2017.05021.

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50

John, C., T. Herz, J. Boos, K. Langer, and G. Hempel. "Asymmetrical flow field-flow fractionation for the analysis of PEG-asparaginase." Talanta 146 (January 2016): 335–39. http://dx.doi.org/10.1016/j.talanta.2015.08.028.

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