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1

Yohannes, G., S. K. Wiedmer, M. Jussila, and M. L. Riekkola. "Fractionation of Humic Substances by Asymmetrical Flow Field-Flow Fractionation." Chromatographia 61, no. 7-8 (March 4, 2005): 359–64. http://dx.doi.org/10.1365/s10337-005-0510-2.

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2

Hee Moon, Myeong, P. Stephen Williams, Dukjin Kang, and Inmi Hwang. "Field and flow programming in frit-inlet asymmetrical flow field-flow fractionation." Journal of Chromatography A 955, no. 2 (May 2002): 263–72. http://dx.doi.org/10.1016/s0021-9673(02)00226-1.

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3

Kang, Dukjin, and Myeong Hee Moon. "Miniaturization of Frit Inlet Asymmetrical Flow Field-Flow Fractionation." Analytical Chemistry 76, no. 13 (July 2004): 3851–55. http://dx.doi.org/10.1021/ac0496704.

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4

Moon, Myeong Hee, and Inmi Hwang. "HYDRODYNAMIC VS. FOCUSING RELAXATION IN ASYMMETRICAL FLOW FIELD-FLOW FRACTIONATION." Journal of Liquid Chromatography & Related Technologies 24, no. 20 (December 31, 2001): 3069–83. http://dx.doi.org/10.1081/jlc-100107720.

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5

LIU, Pan-Pan, Can QUAN, Hong-Mei LI, and Jun-Su JIN. "Characterization of Nanoparticle Diameter by Asymmetrical Flow Field-Flow Fractionation." CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION) 41, no. 7 (2013): 1063. http://dx.doi.org/10.3724/sp.j.1096.2013.21224.

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6

Andreev, Victor P., Yuri V. Stepanov, and J. C. Giddings. "Field-flow fractionation with asymmetrical electroosmotic flow. I. Uncharged particles." Journal of Microcolumn Separations 9, no. 3 (1997): 163–68. http://dx.doi.org/10.1002/(sici)1520-667x(1997)9:3<163::aid-mcs4>3.0.co;2-#.

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7

Andreev, V. P., and Y. V. Stepanov. "Field-Flow Fractionation with Asymmetrical Electroosmotic Flow. II. Charged Particles." Journal of Liquid Chromatography & Related Technologies 20, no. 16-17 (September 1997): 2873–86. http://dx.doi.org/10.1080/10826079708005598.

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8

Oh, Sunok, Dukjin Kang, Sung-Min Ahn, Richard J. Simpson, Bong-Hee Lee, and Myeong Hee Moon. "Miniaturized asymmetrical flow field-flow fractionation: Application to biological vesicles." Journal of Separation Science 30, no. 7 (May 2007): 1082–87. http://dx.doi.org/10.1002/jssc.200600394.

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9

Osorio-Macías, Daniel E., Dongsup Song, Johan Thuvander, Raúl Ferrer-Gallego, Jaeyeong Choi, J. Mauricio Peñarrieta, Lars Nilsson, Seungho Lee, and Björn Bergenståhl. "Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation." Journal of Agricultural and Food Chemistry 68, no. 49 (November 25, 2020): 14564–76. http://dx.doi.org/10.1021/acs.jafc.9b07251.

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10

Yang, Joon Seon, and Myeong Hee Moon. "Flow optimisations with increased channel thickness in asymmetrical flow field-flow fractionation." Journal of Chromatography A 1581-1582 (December 2018): 100–104. http://dx.doi.org/10.1016/j.chroma.2018.10.053.

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11

Liang, Qihui, Di Wu, Bailing Qiu, and Nanyin Han. "Present situation and development trends of asymmetrical flow field-flow fractionation." Chinese Journal of Chromatography 35, no. 9 (2017): 918. http://dx.doi.org/10.3724/sp.j.1123.2017.05021.

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12

John, C., T. Herz, J. Boos, K. Langer, and G. Hempel. "Asymmetrical flow field-flow fractionation for the analysis of PEG-asparaginase." Talanta 146 (January 2016): 335–39. http://dx.doi.org/10.1016/j.talanta.2015.08.028.

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13

Moon, Myeong Hee, P. Stephen Williams, and Hansun Kwon. "Retention and Efficiency in Frit-Inlet Asymmetrical Flow Field-Flow Fractionation." Analytical Chemistry 71, no. 14 (July 1999): 2657–66. http://dx.doi.org/10.1021/ac990040p.

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14

Schauer, Thadeus. "Symmetrical and Asymmetrical Flow Field-Flow Fractionation for Particle Size Determination." Particle & Particle Systems Characterization 12, no. 6 (December 1995): 284–88. http://dx.doi.org/10.1002/ppsc.19950120606.

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15

Juna, Shazia, and Anton Huber. "Characterisation of normal corn starch using asymmetrical flow field-flow fractionation." Starch - Stärke 64, no. 1 (September 21, 2011): 18–26. http://dx.doi.org/10.1002/star.201100068.

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16

Dubascoux, S., F. Von Der Kammer, I. Le Hécho, M. Potin Gautier, and G. Lespes. "Optimisation of asymmetrical flow field flow fractionation for environmental nanoparticles separation." Journal of Chromatography A 1206, no. 2 (October 2008): 160–65. http://dx.doi.org/10.1016/j.chroma.2008.07.032.

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17

Bria, Carmen R. M., and S. Kim Ratanathanawongs Williams. "Impact of asymmetrical flow field-flow fractionation on protein aggregates stability." Journal of Chromatography A 1465 (September 2016): 155–64. http://dx.doi.org/10.1016/j.chroma.2016.08.037.

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18

Williams, P. Stephen. "Retention ratio and nonequilibrium bandspreading in asymmetrical flow field-flow fractionation." Analytical and Bioanalytical Chemistry 407, no. 15 (May 8, 2015): 4327–38. http://dx.doi.org/10.1007/s00216-015-8734-y.

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19

Coelho, Christian, Jérémie Parot, Michael Gonsior, Maria Nikolantonaki, Philippe Schmitt-Kopplin, Edith Parlanti, and Régis D. Gougeon. "Asymmetrical flow field-flow fractionation of white wine chromophoric colloidal matter." Analytical and Bioanalytical Chemistry 409, no. 10 (February 8, 2017): 2757–66. http://dx.doi.org/10.1007/s00216-017-0221-1.

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20

Marioli, Maria, and Wim Th Kok. "Recovery, overloading, and protein interactions in asymmetrical flow field-flow fractionation." Analytical and Bioanalytical Chemistry 411, no. 11 (February 21, 2019): 2327–38. http://dx.doi.org/10.1007/s00216-019-01673-w.

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21

Yohannes, Gebrenegus, Susanne K. Wiedmer, Esa K. J. Tuominen, Paavo K. J. Kinnunen, and Marja-Liisa Riekkola. "Cytochrome c?dimyristoylphosphatidylglycerol interactions studied by asymmetrical flow field-flow fractionation." Analytical and Bioanalytical Chemistry 380, no. 5-6 (November 2004): 757–66. http://dx.doi.org/10.1007/s00216-004-2842-4.

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22

Häusele, Benedikt, Maxim B. Gindele, and Helmut Cölfen. "Revision of the calibration experiment in asymmetrical flow field-flow fractionation." Journal of Chromatography A 1635 (January 2021): 461631. http://dx.doi.org/10.1016/j.chroma.2020.461631.

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23

Moon, Myeong Hee, Hansun Kwon, and Ilyong Park. "Stopless Flow Injection in Asymmetrical Flow Field-Flow Fractionation Using a Frit Inlet." Analytical Chemistry 69, no. 7 (April 1997): 1436–40. http://dx.doi.org/10.1021/ac960897b.

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24

Williams, P. Stephen. "Design of an asymmetrical flow field-flow fractionation channel for uniform channel flow velocity." Journal of Microcolumn Separations 9, no. 6 (1997): 459–67. http://dx.doi.org/10.1002/(sici)1520-667x(1997)9:6<459::aid-mcs3>3.0.co;2-0.

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25

Berger, Martin, Christian Scherer, Sergey Noskov, Christoph Bantz, Conrad Nickel, Wolfgang Schupp, and Michael Maskos. "Influence of oscillating main flow on separation efficiency in asymmetrical flow field-flow fractionation." Journal of Chromatography A 1640 (March 2021): 461941. http://dx.doi.org/10.1016/j.chroma.2021.461941.

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26

Prestel, Harald, Reinhard Niessner, and Ulrich Panne. "Increasing the Sensitivity of Asymmetrical Flow Field-Flow Fractionation: Slot Outlet Technique." Analytical Chemistry 78, no. 18 (September 2006): 6664–69. http://dx.doi.org/10.1021/ac060259l.

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27

Sitar, Simona, Anja Kejžar, David Pahovnik, Ksenija Kogej, Magda Tušek-Žnidarič, Metka Lenassi, and Ema Žagar. "Size Characterization and Quantification of Exosomes by Asymmetrical-Flow Field-Flow Fractionation." Analytical Chemistry 87, no. 18 (August 25, 2015): 9225–33. http://dx.doi.org/10.1021/acs.analchem.5b01636.

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28

Suslov, S. A., and A. J. Roberts. "Modeling of Sample Dynamics in Rectangular Asymmetrical Flow Field-Flow Fractionation Channels." Analytical Chemistry 72, no. 18 (September 2000): 4331–45. http://dx.doi.org/10.1021/ac9915022.

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29

Eskelin, Katri, Minna M. Poranen, and Hanna M. Oksanen. "Asymmetrical Flow Field-Flow Fractionation on Virus and Virus-Like Particle Applications." Microorganisms 7, no. 11 (November 12, 2019): 555. http://dx.doi.org/10.3390/microorganisms7110555.

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Abstract:
Asymmetrical flow field-flow fractionation (AF4) separates sample components based on their sizes in the absence of a stationary phase. It is well suited for high molecular weight samples such as virus-sized particles. The AF4 experiment can potentially separate molecules within a broad size range (~103−109 Da; particle diameter from 2 nm to 0.5−1 μm). When coupled to light scattering detectors, it enables rapid assays on the size, size distribution, degradation, and aggregation of the studied particle populations. Thus, it can be used to study the quality of purified viruses and virus-like particles. In addition to being an advanced analytical characterization technique, AF4 can be used in a semi-preparative mode. Here, we summarize and provide examples on the steps that need optimization for obtaining good separation with the focus on virus-sized particles.
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30

Ciric, Jelena, Agnès Rolland-Sabaté, Sophie Guilois, and Katja Loos. "Characterization of enzymatically synthesized amylopectin analogs via asymmetrical flow field flow fractionation." Polymer 55, no. 24 (November 2014): 6271–77. http://dx.doi.org/10.1016/j.polymer.2014.09.059.

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31

Maskos, Michael, and Wolfgang Schupp. "Circular Asymmetrical Flow Field-Flow Fractionation for the Semipreparative Separation of Particles." Analytical Chemistry 75, no. 22 (November 2003): 6105–8. http://dx.doi.org/10.1021/ac034394z.

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32

Lohrke, Jessica, Andreas Briel, and Karsten Mäder. "Characterization of superparamagnetic iron oxide nanoparticles by asymmetrical flow-field-flow-fractionation." Nanomedicine 3, no. 4 (August 2008): 437–52. http://dx.doi.org/10.2217/17435889.3.4.437.

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33

Scherer, Christian, Sergey Noskov, Stefanie Utech, Christoph Bantz, Waltraut Mueller, Korinna Krohne, and Michael Maskos. "Characterization of Polymer Nanoparticles by Asymmetrical Flow Field Flow Fractionation (AF-FFF)." Journal of Nanoscience and Nanotechnology 10, no. 10 (October 1, 2010): 6834–39. http://dx.doi.org/10.1166/jnn.2010.2973.

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34

Moon, Myeong Hee, Hansun Kwon, and Ilyong Park. "Stopless Separation of Proteins by Frit-Inlet Asymmetrical Flow Field-Flow Fractionation." Journal of Liquid Chromatography & Related Technologies 20, no. 16-17 (September 1997): 2803–14. http://dx.doi.org/10.1080/10826079708005593.

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35

Ahn, Ji Yeon, Ki Hun Kim, Ju Yong Lee, P. Stephen Williams, and Myeong Hee Moon. "Effect of asymmetrical flow field-flow fractionation channel geometry on separation efficiency." Journal of Chromatography A 1217, no. 24 (June 2010): 3876–80. http://dx.doi.org/10.1016/j.chroma.2010.04.021.

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36

Ashby, Jonathan, Samantha Schachermeyer, Yaokai Duan, Luis A. Jimenez, and Wenwan Zhong. "Probing and quantifying DNA–protein interactions with asymmetrical flow field-flow fractionation." Journal of Chromatography A 1358 (September 2014): 217–24. http://dx.doi.org/10.1016/j.chroma.2014.07.002.

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37

Dou, Haiyang, Euo Chang Jung, and Seungho Lee. "Factors affecting measurement of channel thickness in asymmetrical flow field-flow fractionation." Journal of Chromatography A 1393 (May 2015): 115–21. http://dx.doi.org/10.1016/j.chroma.2015.03.025.

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38

Fraunhofer, Wolfgang, and Gerhard Winter. "The use of asymmetrical flow field-flow fractionation in pharmaceutics and biopharmaceutics." European Journal of Pharmaceutics and Biopharmaceutics 58, no. 2 (September 2004): 369–83. http://dx.doi.org/10.1016/j.ejpb.2004.03.034.

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39

Bria, Carmen R. M., Farsad Afshinnia, Patrick W. Skelly, Thekkelnaycke M. Rajendiran, Pradeep Kayampilly, Thommey P. Thomas, Victor P. Andreev, Subramaniam Pennathur, and S. Kim Ratanathanawongs Williams. "Asymmetrical flow field-flow fractionation for improved characterization of human plasma lipoproteins." Analytical and Bioanalytical Chemistry 411, no. 3 (November 23, 2018): 777–86. http://dx.doi.org/10.1007/s00216-018-1499-3.

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40

Lampi, Mirka, Hanna M. Oksanen, Florian Meier, Evelin Moldenhauer, Minna M. Poranen, Dennis H. Bamford, and Katri Eskelin. "Asymmetrical flow field-flow fractionation in purification of an enveloped bacteriophage ϕ6." Journal of Chromatography B 1095 (September 2018): 251–57. http://dx.doi.org/10.1016/j.jchromb.2018.07.008.

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41

Moon, Myeong Hee, Dukjin Kang, Jaehong Jung, and Jongmin Kim. "Separation of carbon nanotubes by frit inlet asymmetrical flow field-flow fractionation." Journal of Separation Science 27, no. 9 (June 2004): 710–17. http://dx.doi.org/10.1002/jssc.200401743.

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42

Eskelin, K., HM Oksanen, and MM Poranen. "Sample carryover and cleaning procedures for asymmetrical flow field-flow fractionation instrument." Journal of Chromatography B 1181 (September 2021): 122920. http://dx.doi.org/10.1016/j.jchromb.2021.122920.

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43

Mukherjee, Arnab, and Vincent A. Hackley. "Separation and characterization of cellulose nanocrystals by multi-detector asymmetrical-flow field-flow fractionation." Analyst 143, no. 3 (2018): 731–40. http://dx.doi.org/10.1039/c7an01739a.

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44

Williams, P. Stephen. "Fractionating power and outlet stream polydispersity in asymmetrical flow field-flow fractionation. Part I: isocratic operation." Analytical and Bioanalytical Chemistry 408, no. 12 (February 13, 2016): 3247–63. http://dx.doi.org/10.1007/s00216-016-9388-0.

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45

Williams, P. Stephen. "Fractionating power and outlet stream polydispersity in asymmetrical flow field-flow fractionation. Part II: programmed operation." Analytical and Bioanalytical Chemistry 409, no. 1 (November 12, 2016): 317–34. http://dx.doi.org/10.1007/s00216-016-0007-x.

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46

Kim, Young Beom, Joon Seon Yang, and Myeong Hee Moon. "Investigation of steric transition with field programming in frit inlet asymmetrical flow field-flow fractionation." Journal of Chromatography A 1576 (November 2018): 131–36. http://dx.doi.org/10.1016/j.chroma.2018.09.036.

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47

Wahlund, Karl Gustav, and J. Calvin Giddings. "Properties of an asymmetrical flow field-flow fractionation channel having one permeable wall." Analytical Chemistry 59, no. 9 (May 1987): 1332–39. http://dx.doi.org/10.1021/ac00136a016.

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48

Marioli, Maria, and Wim Th Kok. "Continuous asymmetrical flow field-flow fractionation for the purification of proteins and nanoparticles." Separation and Purification Technology 242 (July 2020): 116744. http://dx.doi.org/10.1016/j.seppur.2020.116744.

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49

Jungmann, Nadja, Manfred Schmidt, and Michael Maskos. "Characterization of Polyorganosiloxane Nanoparticles in Aqueous Dispersion by Asymmetrical Flow Field-Flow Fractionation." Macromolecules 34, no. 23 (November 2001): 8347–53. http://dx.doi.org/10.1021/ma0106752.

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50

Hawe, Andrea, Stefan Romeijn, Vasco Filipe, and Wim Jiskoot. "Asymmetrical Flow Field-Flow Fractionation Method for the Analysis of Submicron Protein Aggregates." Journal of Pharmaceutical Sciences 101, no. 11 (November 2012): 4129–39. http://dx.doi.org/10.1002/jps.23298.

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