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Journal articles on the topic 'ATP'

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Published: 4 June 2021

Last updated: 25 July 2025

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1

WATANABE, Kimiko, Satsuki TOMIOKA, Kiyoko TANIMURA, Hisae OKU, and Koichiro ISOI. "Uptake of AMP, ADP, and ATP inEscherichia coliW." Bioscience, Biotechnology, and Biochemistry 75, no. 1 (2011): 7–12. http://dx.doi.org/10.1271/bbb.100063.

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2

Schulte, P. M., C. D. Moyes, and P. W. Hochachka. "Integrating metabolic pathways in post-exercise recovery of white muscle." Journal of Experimental Biology 166, no. 1 (1992): 181–95. http://dx.doi.org/10.1242/jeb.166.1.181.

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Purine nucleotides (ATP, ADP, AMP, IMP), creatine, phosphocreatine, lactate, pyruvate and glycogen were measured in rainbow trout (Oncorhynchus mykiss) white muscle following exercise to exhaustion. Estimates of intracellular pH permitted calculation of free concentrations of nucleotides ([nucleotide]f) required for most models of control of energy metabolism. Creatine charge, [PCr]/([PCr]+[Cr]), fell from 0.49 +/− 0.05 (mean +/− S.E.M.) to 0.08 +/− 0.02 with exercise but recovered completely by the first sample (2 h). Although [ATP] declined to 24% of resting levels and recovered very slowly,

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3

Schultz, V., I. Sussman, K. Bokvist, and K. Tornheim. "Bioluminometric Assay of ADP and ATP at High ATP/ADP Ratios: Assay of ADP After Enzymatic Removal of ATP." Analytical Biochemistry 215, no. 2 (1993): 302–4. http://dx.doi.org/10.1006/abio.1993.1591.

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4

Duval, M., A. R. Beaudoin, G. Bkaily, F. P. Gendron, and P. D'Orléans-Juste. "Characterization of the NTPDase activities in the mesentery pre- and post-capillary circuits of the guinea pig." Canadian Journal of Physiology and Pharmacology 81, no. 3 (2003): 212–19. http://dx.doi.org/10.1139/y03-043.

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NTPDase is one of the principal enzymes involved in the sequential hydrolysis of ATP. In the present study, the presence and functionality of NTPDase in the mesenteric vein and artery were examined. Adenosine triphosphate (ATP) (0.011000 pmol) induces a dose-dependent vasodilation in the isolated arterial and venous mesenteric vasculatures of the guinea pig. Adenosine diphosphate (ADP) (0.011000 pmol) but not adenosine monophosphate (AMP) (0.011000 pmol) induces a similar response in the mesenteric vascular circuit. L-NAME, a nitric oxide synthase inhibitor (200 µM, 30 min), significantly r

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5

BAKKE, MIKIO, and SHIGEYA SUZUKI. "Development of a Novel Hygiene Monitoring System Based on the Detection of Total Adenylate (ATP+ADP+AMP)." Journal of Food Protection 81, no. 5 (2018): 729–37. http://dx.doi.org/10.4315/0362-028x.jfp-17-432.

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ABSTRACT ATP is the universal energy molecule found in animals, plants, and microorganisms. ATP rapid hygiene monitoring tests have been employed in the food industry to ensure that adequate cleanliness is being maintained. However, because ATP is hydrolyzed to ADP and AMP by metabolic processes, by heat treatment, or under acidic or alkaline conditions, total adenylate (ATP+ADP+AMP [A3]) could be a more reliable sanitation indicator of food residues that may cause biofilm formation and allergen contamination. Therefore, a novel hygiene monitoring system to measure A3 was developed based on th

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6

Resnick, Sol M., and Alexander J. B. Zehnder. "In Vitro ATP Regeneration from Polyphosphate and AMP by Polyphosphate:AMP Phosphotransferase and Adenylate Kinase from Acinetobacter johnsonii 210A." Applied and Environmental Microbiology 66, no. 5 (2000): 2045–51. http://dx.doi.org/10.1128/aem.66.5.2045-2051.2000.

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ABSTRACT In vitro enzyme-based ATP regeneration systems are important for improving yields of ATP-dependent enzymatic reactions for preparative organic synthesis and biocatalysis. Several enzymatic ATP regeneration systems have been described but have some disadvantages. We report here on the use of polyphosphate:AMP phosphotransferase (PPT) fromAcinetobacter johnsonii strain 210A in an ATP regeneration system based on the use of polyphosphate (polyP) and AMP as substrates. We have examined the substrate specificity of PPT and demonstrated ATP regeneration from AMP and polyP using firefly luci

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7

Vetri, Francesco, Haoliang Xu, Lizhen Mao, Chanannait Paisansathan, and Dale A. Pelligrino. "ATP hydrolysis pathways and their contributions to pial arteriolar dilation in rats." American Journal of Physiology-Heart and Circulatory Physiology 301, no. 4 (2011): H1369—H1377. http://dx.doi.org/10.1152/ajpheart.00556.2011.

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ATP is thought to be released to the extracellular compartment by neurons and astrocytes during neural activation. We examined whether ATP exerts its effect of promoting pial arteriolar dilation (PAD) directly or upon conversion (via ecto-nucleotidase action) to AMP and adenosine. Blockade of extracellular direct ATP to AMP conversion, with ARL-67156, significantly reduced sciatic nerve stimulation-evoked PADs by 68%. We then monitored PADs during suffusions of ATP, ADP, AMP, and adenosine in the presence and absence of the following: 1) the ecto-5′-nucleotidase inhibitor α,β-methylene adenosi

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8

Chen, G., and H. Suzuki. "Endothelium-dependent hyperpolarization elicited by adenine compounds in rabbit carotid artery." American Journal of Physiology-Heart and Circulatory Physiology 260, no. 4 (1991): H1037—H1042. http://dx.doi.org/10.1152/ajpheart.1991.260.4.h1037.

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Electrical responses of the membrane of intimal and adventitial smooth muscle cells of the rabbit carotid artery to ATP, ADP, AMP, and adenosine were recorded. In intimal cells, these compounds hyperpolarized the membrane. Mechanical removal of the endothelium altered the responses to ATP and ADP to one of a transient depolarization, with no alteration of the response to AMP and adenosine. In the adventitial cells, ATP and ADP produced a transient depolarization, whereas AMP and adenosine produced a sustained hyperpolarization, irrespective of the presence or absence of the endothelium. In tis

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9

Warburton, D., S. Buckley, and L. Cosico. "P1 and P2 purinergic receptor signal transduction in rat type II pneumocytes." Journal of Applied Physiology 66, no. 2 (1989): 901–5. http://dx.doi.org/10.1152/jappl.1989.66.2.901.

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Extracellular ATP is a potent agonist of surfactant phosphatidylcholine (PC) exocytosis from type II pneumocytes in culture. We studied P1 and P2 receptor signal transduction in type II pneumocytes. The EC50 for ATP on PC exocytosis was 10(-6) M, whereas the EC50 for ADP, AMP, adenosine, and the nonmetabolizable ATP analogue alpha,beta-methylene ATP was 10(-4) M. The rank order of agonists for PC exocytosis was ATP greater than ADP greater than AMP greater than adenosine greater than alpha,beta-methylene ATP. The rank order of agonists for phosphatidylinositol (PI) hydrolysis was ATP greater t

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10

Ko, W. H., J. J. O'Dowd, J. D. Pediani, et al. "Extracellular ATP can activate autonomic signal transduction pathways in cultured equine sweat gland epithelial cells." Journal of Experimental Biology 190, no. 1 (1994): 239–52. http://dx.doi.org/10.1242/jeb.190.1.239.

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Changes in intracellular free calcium concentration ([Ca2+]i) were monitored in a cell line that was derived from the equine sweat gland epithelium. ATP and closely related compounds could increase [Ca2+]i with a rank order of potency of UTP &gt; or = ATP &gt; ADP &gt; AMP = adenosine = alpha,beta-methylene-ATP. The responses to ATP and to UTP were initiated by the release of calcium from an internal store and subsequently sustained by calcium influx. The rise in [Ca2+]i thus seems to be mediated by P2U receptors that are coupled to phosphoinositidase C. Some desensitisation of thi

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11

Pinna, Silvana, Cäcilia Kunz, Aaron Halpern, et al. "A prebiotic basis for ATP as the universal energy currency." PLOS Biology 20, no. 10 (2022): e3001437. http://dx.doi.org/10.1371/journal.pbio.3001437.

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ATP is universally conserved as the principal energy currency in cells, driving metabolism through phosphorylation and condensation reactions. Such deep conservation suggests that ATP arose at an early stage of biochemical evolution. Yet purine synthesis requires 6 phosphorylation steps linked to ATP hydrolysis. This autocatalytic requirement for ATP to synthesize ATP implies the need for an earlier prebiotic ATP equivalent, which could drive protometabolism before purine synthesis. Why this early phosphorylating agent was replaced, and specifically with ATP rather than other nucleoside tripho

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12

Randak, Christoph O., Qian Dong, Amanda R. Ver Heul, Adrian H. Elcock, and Michael J. Welsh. "ATP and AMP Mutually Influence Their Interaction with the ATP-binding Cassette (ABC) Adenylate Kinase Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) at Separate Binding Sites." Journal of Biological Chemistry 288, no. 38 (2013): 27692–701. http://dx.doi.org/10.1074/jbc.m113.479675.

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Cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel in the ATP-binding cassette (ABC) transporter protein family. In the presence of ATP and physiologically relevant concentrations of AMP, CFTR exhibits adenylate kinase activity (ATP + AMP ⇆ 2 ADP). Previous studies suggested that the interaction of nucleotide triphosphate with CFTR at ATP-binding site 2 is required for this activity. Two other ABC proteins, Rad50 and a structural maintenance of chromosome protein, also have adenylate kinase activity. All three ABC adenylate kinases bind and hydrolyze ATP in the abse

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13

Meghji, P., J. D. Pearson, and L. L. Slakey. "Kinetics of extracellular ATP hydrolysis by microvascular endothelial cells from rat heart." Biochemical Journal 308, no. 3 (1995): 725–31. http://dx.doi.org/10.1042/bj3080725.

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We have characterized the ectonucleotidases that catalyse the reaction sequence ATP-->ADP-->AMP-->adenosine on microvascular endothelial cells cultured from the rat heart. Computer simulation and data fitting of progress of reaction curves showed that depletion of substrate at the cell surface dominates the regulation of the rate of hydrolysis of ATP when it is presented to the cells. Preferential delivery of AMP by ADPase to 5′-nucleotidase makes a significant contribution to the regulation of adenosine production from ATP or ADP. By contrast, we found no evidence for the preferentia

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14

Li, Min-ji, Qin-ping Wei, Fu-tian Peng, Wen Yu, Jing-jing Luo, and Yong-fei Zhao. "Identification and Characterization of ATP/ADP Isopentenyltransferases (ATP/ADP PpIPTs) Genes in Peach." Journal of Plant Growth Regulation 38, no. 2 (2018): 416–30. http://dx.doi.org/10.1007/s00344-018-9851-6.

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15

Hardie, D. Grahame. "Keeping the home fires burning: AMP-activated protein kinase." Journal of The Royal Society Interface 15, no. 138 (2018): 20170774. http://dx.doi.org/10.1098/rsif.2017.0774.

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Living cells obtain energy either by oxidizing reduced compounds of organic or mineral origin or by absorbing light. Whichever energy source is used, some of the energy released is conserved by converting adenosine diphosphate (ADP) to adenosine triphosphate (ATP), which are analogous to the chemicals in a rechargeable battery. The energy released by the conversion of ATP back to ADP is used to drive most energy-requiring processes, including cell growth, cell division, communication and movement. It is clearly essential to life that the production and consumption of ATP are always maintained

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16

Fleetwood, G., S. B. Coade, J. L. Gordon, and J. D. Pearson. "Kinetics of adenine nucleotide catabolism in coronary circulation of rats." American Journal of Physiology-Heart and Circulatory Physiology 256, no. 6 (1989): H1565—H1572. http://dx.doi.org/10.1152/ajpheart.1989.256.6.h1565.

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We have used the rat isolated, perfused heart to study the metabolism of adenine nucleotides on a single passage through the coronary circulation. Low doses (3-30 nmol) of ATP, ADP, or AMP injected as a bolus were extensively catabolized by ectoenzymes. Increasing doses of each nucleotide demonstrated saturability of catabolism that occurred at significantly lower doses of AMP than of ADP or ATP. The patterns of catabolites formed in each case were consistent with the major pathway of metabolism being sequential dephosphorylation of ATP----ADP----AMP----adenosine, although from experiments in

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17

Gowans, Graeme J., and D. Grahame Hardie. "AMPK: a cellular energy sensor primarily regulated by AMP." Biochemical Society Transactions 42, no. 1 (2014): 71–75. http://dx.doi.org/10.1042/bst20130244.

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AMPK (AMP-activated protein kinase) is a cellular energy sensor that monitors the ratio of AMP/ATP, and possibly also ADP/ATP, inside cells. Once activated by falling cellular energy levels, it acts to restore energy homoeostasis by switching on catabolic pathways that generate ATP, while switching off anabolic pathways and other processes consuming ATP. AMPK is switched on by increases in AMP via three mechanisms, all of which are antagonized by ATP: (i) promotion of phosphorylation of Thr172 by upstream activating kinases; (ii) inhibition of dephosphorylation of Thr172 by phosphatases; and (

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18

Dho, S., K. Stewart, and J. K. Foskett. "Purinergic receptor activation of Cl- secretion in T84 cells." American Journal of Physiology-Cell Physiology 262, no. 1 (1992): C67—C74. http://dx.doi.org/10.1152/ajpcell.1992.262.1.c67.

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The regulation by ATP of Cl- secretion in T84 cells grown on filters was investigated by measuring short-circuit current (Isc = net Cl- secretion). ATP (greater than or equal to 10 microM) added to the basolateral side markedly stimulated Isc both in the presence and absence of forskolin-activated Isc. Fluorescence microscopy of cells loaded with the Ca2+ indicator fura-2 showed that ATP stimulated a transient increase in intracellular free Ca2+ concentration [Ca2+]i. The augmentation of forskolin-stimulated Isc by ATP was at least partly caused by mobilization of Ca2+ from an internal store b

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19

VALERO, Edelmira, Ramón VARÓN, and Francisco GARCÍA-CARMONA. "Kinetics of a self-amplifying substrate cycle: ADP–ATP cycling assay." Biochemical Journal 350, no. 1 (2000): 237–43. http://dx.doi.org/10.1042/bj3500237.

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A kinetic study of an ATP–ADP amplification cyclic system involving the enzymes adenylate kinase, pyruvate kinase and l-lactate dehydrogenase has been made. The stoichiometry of the cycle is 2:1, because two molecules of ADP are synthesized from one each of ATP and AMP, and one molecule of ADP is converted back into one of ATP at each turn of the cycle. This results in a continuous exponential increase in the concentrations of ATP and ADP in the reaction medium, according to the equations obtained. This is therefore a substrate cycle that amplifies itself, the cycling rate increasing continuou

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20

Mysin, I. E., I. Yu Popova, and A. A. Osipov. "The Mathematical Model of Disturbance of Energy Metabolism in Brain during Development of Neurodegenerative Diseases: A Proposed Mechanism of Cell Death." Mathematical Biology and Bioinformatics 13, no. 2 (2018): 591–608. http://dx.doi.org/10.17537/2018.13.591.

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The paper presents a theoretical study of the failure of energy metabolism in nervous tissue on a detailed biophysical model of metabolic coupling between neurons and astrocytes under afferent stimulation simulating by the release of glutamate into the presynaptic cleft. The main result of the model study is the detection of the phenomenon of adenylate collapse, the essence of which is the irreversible drop of ATP concentration after reaching the critical threshold value. This effect occurs as a result of the reversing of the reaction catalyzed by adenylate kinase. At high ATP values, the reac

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21

Asensi, M., A. Lopez-Rodas, J. Sastre, J. Vina, and J. M. Estrela. "Inhibition of gluconeogenesis by extracellular ATP in isolated rat hepatocytes." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 261, no. 6 (1991): R1522—R1526. http://dx.doi.org/10.1152/ajpregu.1991.261.6.r1522.

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The aim of this study was to determine the effect of externally added ATP on gluconeogenesis by isolated hepatocytes from starved rats. High concentrations of extracellular ATP inhibited gluconeogenesis from lactate and pyruvate but not from glycerol or fructose. This inhibition was associated with an increase in intracellular adenosine contents. ADP, AMP, or adenosine but not guanosine 5'triphosphate, inosine 5' triphosphate, or adenine also inhibited gluconeogenesis. alpha, beta-Methylene-ATP, a nonmetabolizable structural analogue of ATP, did not affect the rate of gluconeogenesis. Intracel

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22

Markaryan, Adam, Olga Zaborina, Vasu Punj, and A. M. Chakrabarty. "Adenylate Kinase as a Virulence Factor ofPseudomonas aeruginosa." Journal of Bacteriology 183, no. 11 (2001): 3345–52. http://dx.doi.org/10.1128/jb.183.11.3345-3352.2001.

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ABSTRACT Adenylate kinase (AK; ATP:AMP phosphotransferase, EC 2.7.4.3 ) is a ubiquitous enzyme that contributes to the homeostasis of adenine nucleotides in eukaryotic and prokaryotic cells. AK catalyzes the reversible reaction Mg · ATP + AMP ↔ Mg · ADP + ADP. In this study we show that AK secreted by the pathogenic strains of Pseudomonas aeruginosa appears to play an important role in macrophage cell death. We purified and characterized AK from the growth medium of a cystic fibrosis isolate strain of P. aeruginosa 8821 and hyperproduced it as a fusion protein with glutathione S-transferase. W

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23

Popovic, Zvezdana, and Douglas M. Templeton. "Interaction of iron regulatory protein-1 (IRP-1) with ATP/ADP maintains a non-IRE-binding state." Biochemical Journal 430, no. 2 (2010): 315–24. http://dx.doi.org/10.1042/bj20100111.

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In its aconitase-inactive form, IRP-1 (iron regulatory protein-1)/cytosolic aconitase binds to the IRE (iron-responsive element) of several mRNAs to effect post-transcriptional regulation. We have shown previously that IRP-1 has ATPase activity and that binding of ATP suppresses the IRP-1/IRE interaction. In the present study, we characterize the binding activity further. Binding is observed with both [α-32P]ATP and [α-32P]ADP, but not with [γ-32P]ATP. Recombinant IRP-1 binds approximately two molecules of ATP, and positive co-operativity is observed with a Hill coefficient of 1.67±0.36 (EC50=

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Meghji, P., J. D. Pearson, and L. L. Slakey. "Regulation of extracellular adenosine production by ectonucleotidases of adult rat ventricular myocytes." American Journal of Physiology-Heart and Circulatory Physiology 263, no. 1 (1992): H40—H47. http://dx.doi.org/10.1152/ajpheart.1992.263.1.h40.

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We have investigated the kinetic properties of the extracellular reaction sequence ATP----ADP----AMP----adenosine catalyzed by ectonucleotidases at the surface of adult rat cardiac myocytes. Analysis of progress of reaction curves indicates that depletion of substrate at cell surfaces dominates the regulation of the rate of hydrolysis of ATP or of ADP when it is the initial substrate. Preferential delivery of intermediate products to be substrates at cell surfaces makes a significant contribution to the regulation of adenosine production from ATP or ADP. Preferential delivery has more impact o

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25

Wilson, David F., and Franz M. Matschinsky. "Metabolic homeostasis: oxidative phosphorylation and the metabolic requirements of higher plants and animals." Journal of Applied Physiology 125, no. 4 (2018): 1183–92. http://dx.doi.org/10.1152/japplphysiol.00352.2018.

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A model of oxidative phosphorylation and its regulation is presented, which is consistent with the experimental data on metabolism in higher plants and animals. The variables that provide real-time control of metabolic status are: intramitochondrial [NAD+]/[NADH], energy state ([ATP]/[ADP][Pi]), and local oxygen concentration ([O2]). ATP consumption and respiratory chain enzyme content are tissue specific (liver vs. heart muscle), and the latter is modulated by chronic alterations in ATP consumption (i.e., endurance training etc.). ATP consumption affects the energy state, which increases or d

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26

Katchanov, G., J. Xu, A. Clay, and A. Pelleg. "Electrophysiological-anatomic correlates of ATP-triggered vagal reflex in the dog. IV. Role of LV vagal afferents." American Journal of Physiology-Heart and Circulatory Physiology 272, no. 4 (1997): H1898—H1903. http://dx.doi.org/10.1152/ajpheart.1997.272.4.h1898.

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The negative chronotropic action and the time to peak effect (t(p)) of ATP and its related analogs [2-methylthio-ATP (2-MeSATP), alpha,beta-methylene-ATP (alpha,beta-mATP), and beta,gamma-methylene-ATP (beta,gamma-mATP)] as well as ADP, AMP, and adenosine were determined in anesthetized dogs. Intra-right atrium (RA) and intra-left main coronary artery (LM) ATP markedly suppressed sinus node automaticity. ATP induced a much greater response when administered into the LM than into the RA. The t(p) of ATP administered at the former site was much shorter than that at the latter site. Intra-LM aden

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27

Monterrey, Dianelis T., Leire Azcona, Julia Revuelta, Israel Sánchez-Moreno, and Eduardo García-Junceda. "Polyphosphate Kinase from Burkholderia cenocepacia, One Enzyme Catalyzing a Two-Step Cascade Reaction to Synthesize ATP from AMP." International Journal of Molecular Sciences 25, no. 23 (2024): 12995. https://doi.org/10.3390/ijms252312995.

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This study characterizes a novel polyphosphate kinase from Burkholderia cenocepacia (BcPPK2-III), an enzyme with potential applications in ATP regeneration processes. Bioinformatic and structural analyses confirmed the presence of conserved motifs characteristic of PPK2 enzymes, including Walker A and B motifs, and the subclass-specific residue E137. Molecular docking simulations showed AMP had the highest binding affinity (−7.0 kcal/mol), followed by ADP (−6.5 kcal/mol), with ATP having the lowest affinity (−6.3 kcal/mol). It was overexpressed in Escherichia coli, after purification enzymatic

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28

Buxton, D. B., S. M. Robertson, and M. S. Olson. "Stimulation of glycogenolysis by adenine nucleotides in the perfused rat liver." Biochemical Journal 237, no. 3 (1986): 773–80. http://dx.doi.org/10.1042/bj2370773.

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Infusion of adenine nucleotides and adenosine into perfused rat livers resulted in stimulation of hepatic glycogenolysis, transient increases in the effluent perfusate [3-hydroxybutyrate]/[acetoacetate] ratio, and increased portal vein pressure. In livers perfused with buffer containing 50 microM-Ca2+, transient efflux of Ca2+ was seen on stimulation of the liver with adenine nucleotides or adenosine. ADP was the most potent of the nucleotides, stimulating glucose output at concentrations as low as 0.15 microM, with half-maximal stimulation at approx. 1 microM, and ATP was slightly less potent

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29

SMITH, N. W., J. J. SINDELAR, and S. A. RANKIN. "Quantities of Adenylate Homologues (ATP+ADP+AMP) Change over Time in Prokaryotic and Eukaryotic Cells." Journal of Food Protection 82, no. 12 (2019): 2088–93. http://dx.doi.org/10.4315/0362-028x.jfp-19-223.

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ABSTRACT Rapid assays for the assessment of the hygienic state of surfaces in food and medical industries include the use of technologies designed to detect the presence of the metabolite ATP. ATP is a critical metabolite and energy source for most living organisms; therefore, the presence of ATP can be an indicator of surface hygiene based on the presence of soil or food residues associated with inadequate cleaning. The concentrations of ATP vary based on an organism's metabolic state, thus potentially influencing the sensitivity of ATP-based assays. However, little has been published detaili

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30

Weinreich, Frank, John R. Riordan, and Georg Nagel. "Dual Effects of Adp and Adenylylimidodiphosphate on Cftr Channel Kinetics Show Binding to Two Different Nucleotide Binding Sites." Journal of General Physiology 114, no. 1 (1999): 55–70. http://dx.doi.org/10.1085/jgp.114.1.55.

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The CFTR chloride channel is regulated by phosphorylation by protein kinases, especially PKA, and by nucleotides interacting with the two nucleotide binding domains, NBD-A and NBD-B. Giant excised inside-out membrane patches from Xenopus oocytes expressing human epithelial cystic fibrosis transmembrane conductance regulator (CFTR) were tested for their chloride conductance in response to the application of PKA and nucleotides. Rapid changes in the concentration of ATP, its nonhydrolyzable analogue adenylylimidodiphosphate (AMP-PNP), its photolabile derivative ATP-P3-[1-(2-nitrophenyl)ethyl]est

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Bertrand, G., J. Chapal, and M. M. Loubatieres-Mariani. "Potentiating synergism between adenosine diphosphate or triphosphate and acetylcholine on insulin secretion." American Journal of Physiology-Endocrinology and Metabolism 251, no. 4 (1986): E416—E421. http://dx.doi.org/10.1152/ajpendo.1986.251.4.e416.

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The interaction between adenosine di- or triphosphate (ADP or ATP) and acetylcholine (ACh) was studied on insulin secretion. The experiments were performed on the isolated perfused rat pancreas, in the presence of a physiological nonstimulating glucose concentration (5.5 mM). ADP or ATP (1.65 microM) and ACh (0.05 microM) elicited a comparable peak of insulin secretion. When either ATP or ADP was simultaneously infused with ACh, insulin secretory response was significantly higher than the sum of the responses of each agonist applied separately. Similar effects were obtained with stable structu

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32

Gough, N. R. "ADP for ATP Exchange Mechanism." Science's STKE 2007, no. 410 (2007): tw392. http://dx.doi.org/10.1126/stke.4102007tw392.

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33

Kudryashov, Dmitri S., and Emil Reisler. "ATP and ADP actin states." Biopolymers 99, no. 4 (2013): 245–56. http://dx.doi.org/10.1002/bip.22155.

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34

Rouse, D., M. Leite, and W. N. Suki. "ATP inhibits the hydrosmotic effect of AVP in rabbit CCT: evidence for a nucleotide P2u receptor." American Journal of Physiology-Renal Physiology 267, no. 2 (1994): F289—F295. http://dx.doi.org/10.1152/ajprenal.1994.267.2.f289.

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In rabbit renal cortical collecting tubule (CCT), perfused in vitro at 38 degrees C, ATP in concentrations of 10(-7) M and greater inhibits arginine vasopressin (AVP)-stimulated osmotic water permeability (Pf). The P1-purinergic receptor antagonist 8-phenyltheophylline did not attenuate the inhibitory action of ATP, and the poorly hydrolyzable ATP analogue, 5'-adenylylimidodiphosphate (AMP-PNP), mimicked the effect of ATP, arguing against an effect of ATP on a P1 receptor or the "P site." Purinergic receptor agonists inhibited AVP-stimulated Pf with the following rank order efficacy: ATP = ADP

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35

Gorman, Mark W., Kayoko Ogimoto, Margaret V. Savage, Kenneth A. Jacobson, and Eric O. Feigl. "Nucleotide coronary vasodilation in guinea pig hearts." American Journal of Physiology-Heart and Circulatory Physiology 285, no. 3 (2003): H1040—H1047. http://dx.doi.org/10.1152/ajpheart.00981.2002.

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The role of P1 receptors and P2Y1 receptors in coronary vasodilator responses to adenine nucleotides was examined in the isolated guinea pig heart. Bolus arterial injections of nucleotides were made in hearts perfused at constant pressure. Peak increase in flow was measured before and after addition of purinoceptor antagonists. Both the P1 receptor antagonist 8-( p-sulfophenyl)theophylline and adenosine deaminase inhibited adenosine vasodilation. AMP-induced vasodilation was inhibited by P1 receptor blockade but not by adenosine deaminase or by the selective P2Y1 antagonist N6-methyl-2′-deoxya

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36

Hancock, Chad R., Jeffrey J. Brault, Robert W. Wiseman, Ronald L. Terjung, and Ronald A. Meyer. "31P-NMR observation of free ADP during fatiguing, repetitive contractions of murine skeletal muscle lacking AK1." American Journal of Physiology-Cell Physiology 288, no. 6 (2005): C1298—C1304. http://dx.doi.org/10.1152/ajpcell.00621.2004.

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Metabolic control within skeletal muscle is designed to limit ADP accumulation even during conditions where ATP demand is out of balance with ATP synthesis. This is accomplished by the reactions of adenylate kinase (AK; ADP+ADP ↔ AMP+ATP) and AMP deaminase (AMP+H2O → NH3+IMP), which limit ADP accumulation under these conditions. The purpose of this study was to determine whether AK deficiency (AK−/−) would result in sufficient ADP accumulation to be visible using 31P-NMRS during the high energy demands of frequent in situ tetanic contractions. To do this we examined the high-energy phosphates

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Sarkis, J. J. F., J. A. Guimarães, and J. M. C. Ribeiro. "Salivary apyrase of Rhodnius prolixus. Kinetics and purification." Biochemical Journal 233, no. 3 (1986): 885–91. http://dx.doi.org/10.1042/bj2330885.

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The salivary apyrase activity of the blood-sucking bug Rhodnius prolixus was found to reside in a true apyrase (ATP diphosphohydrolase, EC 3.6.1.5) enzyme. The crude saliva was devoid of 5′-nucleotidase, inorganic pyrophosphatase, phosphatase and adenylate kinase activities. ATP hydrolysis proceeded directly to AMP and Pi without significant accumulation of ADP. Km values for ATP and ADP hydrolysis were 229 and 291 microM respectively. Ki values for ATP and ADP inhibition of ADP and ATP hydrolysis were not different from the Km values, and these experiments indicated competitive inhibition. Ac

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Wang, W., and G. Giebisch. "Dual effect of adenosine triphosphate on the apical small conductance K+ channel of the rat cortical collecting duct." Journal of General Physiology 98, no. 1 (1991): 35–61. http://dx.doi.org/10.1085/jgp.98.1.35.

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We used the patch-clamp technique to study the effects of ATP on the small-conductance potassium channel in the apical membrane of rat cortical collecting duct (CCD). This channel has a high open probability (0.96) in the cell-attached mode but activity frequently disappeared progressively within 1-10 min after channel excision (channel "run-down"). Two effects of ATP were observed. Using inside-out patches, low concentrations of ATP (0.05-0.1 mM) restored channel activity in the presence of cAMP-dependent protein kinase A (PKA). In contrast, high concentrations (1 mM) of adenosine triphosphat

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39

Naito, Y., and J. M. Lowenstein. "5′-Nucleotidase from rat heart membranes. Inhibition by adenine nucleotides and related compounds." Biochemical Journal 226, no. 3 (1985): 645–51. http://dx.doi.org/10.1042/bj2260645.

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ADP and ATP and their analogues were evaluated as inhibitors of 5′-nucleotidase purified from heart plasma membrane. ADP analogues are more powerful inhibitors than the corresponding ATP analogues. The most powerful inhibitor found is adenosine 5′-[alpha beta-methylene]diphosphate (AOPCP) for which the enzyme shows a Ki of 5 nM at pH 7.2. Measurements of pKi values for ADP and AOPCP as a function of pH indicate that the major inhibitory species of both nucleotides is the dianion. In the physiological range of pH values, AOPCP is a more powerful inhibitor than ADP principally because a higher p

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40

Balazovich, K. J., and L. A. Boxer. "Extracellular adenosine nucleotides stimulate protein kinase C activity and human neutrophil activation." Journal of Immunology 144, no. 2 (1990): 631–37. http://dx.doi.org/10.4049/jimmunol.144.2.631.

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Abstract We studied the effect of adenosine nucleotides on several aspects of the functional activation of human peripheral blood polymorphonuclear leukocytes (PMN). Radiolabeled ATP bound to PMN in a manner suggesting the existence of specific binding sites because: 1) binding was reversed (92 +/- 6%) by 100-fold excess concentrations of unlabeled ATP but minimally by either ADP (43 +/- 12%) or GTP (37 +/- 8%); and 2) binding saturation was achieved (i.e., specific binding did not increase) above 250 microM ATP. Binding studies revealed that significant ATP hydrolysis occurred, even at low te

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Murgo, AJ, JG Contrera, and FD Sistare. "Evidence for separate calcium-signaling P2T and P2U purinoceptors in human megakaryocytic Dami cells." Blood 83, no. 5 (1994): 1258–67. http://dx.doi.org/10.1182/blood.v83.5.1258.1258.

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Abstract Recently (J Pharmacol Exp Ther 261:580, 1992), we have shown that K562 leukemia cells express a calcium-signaling purinoceptor with characteristics of the P2T receptor subtype for adenosine diphosphate (ADP) previously found only in platelets. Because these results suggested that the P2T receptor may be an early marker for megakaryocytic differentiation, we studied whether this calcium- signaling receptor is also expressed in Dami cells, a human megakaryocytic leukemia cell line. Here we report evidence that Dami cells express a P2T receptor for ADP. The calcium response EC50 values f

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Murgo, AJ, JG Contrera, and FD Sistare. "Evidence for separate calcium-signaling P2T and P2U purinoceptors in human megakaryocytic Dami cells." Blood 83, no. 5 (1994): 1258–67. http://dx.doi.org/10.1182/blood.v83.5.1258.bloodjournal8351258.

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Recently (J Pharmacol Exp Ther 261:580, 1992), we have shown that K562 leukemia cells express a calcium-signaling purinoceptor with characteristics of the P2T receptor subtype for adenosine diphosphate (ADP) previously found only in platelets. Because these results suggested that the P2T receptor may be an early marker for megakaryocytic differentiation, we studied whether this calcium- signaling receptor is also expressed in Dami cells, a human megakaryocytic leukemia cell line. Here we report evidence that Dami cells express a P2T receptor for ADP. The calcium response EC50 values for ADP, 2

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Mo, M., S. G. Eskin, and W. P. Schilling. "Flow-induced changes in Ca2+ signaling of vascular endothelial cells: effect of shear stress and ATP." American Journal of Physiology-Heart and Circulatory Physiology 260, no. 5 (1991): H1698—H1707. http://dx.doi.org/10.1152/ajpheart.1991.260.5.h1698.

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The effect of hemodynamic flow on apparent cytosolic free Ca2+ concentration ([Ca2+]i) of cultured bovine aortic endothelial cells was examined in the absence and presence of adenine nucleotides using microfluorimetric analysis of fura-2 fluorescence. In the absence of adenine nucleotides, flow-induced shear stress produced little change (less than 10 nM) in [Ca2+]i. Similar results were obtained using calf pulmonary artery and human umbilical vein endothelial cells. However, addition of the adenine nucleotides ATP, ADP, or AMP under flow conditions produced a transient peak increase in [Ca2+]

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44

Konduri, Girija G., Ivane Bakhutashvili, Recia Frenn, Indira Chandrasekhar, Elizabeth R. Jacobs, and Ashwani K. Khanna. "P2Y purine receptor responses and expression in the pulmonary circulation of juvenile rabbits." American Journal of Physiology-Heart and Circulatory Physiology 287, no. 1 (2004): H157—H164. http://dx.doi.org/10.1152/ajpheart.00617.2003.

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The purine nucleotide ATP mediates pulmonary vasodilation at birth by stimulation of P2Y purine receptors in the pulmonary circulation. The specific P2Y receptors in the pulmonary circulation and the segmental distribution of their responses remain unknown. We investigated the effects of purine nucleotides, ATP, ADP, and AMP, and pyrimidine nucleotides, UTP, UDP, and UMP, in juvenile rabbit pulmonary arteries for functional characterization of P2Y receptors. We also studied the expression of P2Y receptor subtypes in pulmonary arteries and the role of nitric oxide (NO), prostaglandins, and cyto

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Shanbhag, S. M., and G. R. Choppin. "Thermodynamics of Mg and Ca complexation with AMP, ADP, ATP." Inorganica Chimica Acta 138, no. 3 (1987): 187–92. http://dx.doi.org/10.1016/s0020-1693(00)81221-9.

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Hosseini, Mir Wais, and Jean-Marie Lehn. "Binding of AMP, ADP, and ATP Nucleotides by Polyammonium Macrocycles." Helvetica Chimica Acta 70, no. 5 (1987): 1312–19. http://dx.doi.org/10.1002/hlca.19870700512.

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Zhang, Haoling, Haolong Zhang, Rui Zhao, et al. "ATP Cellotoxicity, ATP-Induced Cell Death and ATP Depletion." International Journal of Public Health and Medical Research 1, no. 2 (2024): 35–38. http://dx.doi.org/10.62051/ijphmr.v1n2.05.

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This paper delves into three pivotal themes concerning adenosine triphosphate (ATP): ATP cytotoxicity, ATP-induced cell death, and ATP depletion. Initially, we scrutinize ATP's involvement in cytotoxicity, with a specific focus on elucidating the mechanisms underlying cell demise triggered by augmented extracellular ATP concentrations. Subsequently, we investigate the process of ATP-induced cell death, emphasizing the repercussions of heightened extracellular ATP levels on cellular dynamics and associated biological responses. Lastly, we probe the impact of ATP depletion on cellular function a

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Guo, Jinli, Guanyu Zhu, Lianguo Li, Huan Liu, and Shuang Liang. "Ultraweak photon emission in strawberry fruit during ripening and aging is related to energy level." Open Life Sciences 12, no. 1 (2017): 393–98. http://dx.doi.org/10.1515/biol-2017-0046.

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AbstractBackgroundUltra-weak photon emission (UPE), or biophoton emission, is a phenomenon observed in various living organisms, including plants. In this study, we analyzed the UPE from ripening strawberry fruits, to elucidate its source and association with cellular energy.MethodsFreshly harvested and stored strawberry fruits were measured for levels of UPE and energy molecules adenosine triphosphate (ATP), adenosine monophosphate (AMP) and adenosine diphosphate (ADP). The associations between them were calculated.ResultsIn ripening fruit, a decrease in UPE positively correlated with declini

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Green, A. K., P. H. Cobbold, and C. J. Dixon. "Elevated intracellular cyclic AMP exerts different modulatory effects on cytosolic free Ca2+ oscillations induced by ADP and ATP in single rat hepatocytes." Biochemical Journal 302, no. 3 (1994): 949–55. http://dx.doi.org/10.1042/bj3020949.

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Single aequorin-injected hepatocytes respond to agonists acting via the phosphoinositide signalling pathway by the generation of oscillations in cytosolic free Ca2+ concentration ([Ca2+]free). The duration of [Ca2+]free transients is characteristic of the stimulating agonist. We have previously reported that ADP and ATP, which are believed to act through a single P(2y)-purinoceptor species, induce very different oscillatory [Ca2+]free responses in the majority of hepatocytes. We have interpreted these data as evidence for two separate Ca(2+)-mobilizing purinoceptors for these nucleotides. We s

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Sun, Tianjun, Koto Hayakawa, and Marie E. Fraser. "ADP–Mg2+bound to the ATP-grasp domain of ATP-citrate lyase." Acta Crystallographica Section F Structural Biology and Crystallization Communications 67, no. 10 (2011): 1168–72. http://dx.doi.org/10.1107/s1744309111028363.

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