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Journal articles on the topic 'Automated DNA extraction'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Sanqoor, Afra Naqib, Fatma Altamimi, Naeema Aljanahi, et al. "Comparative Study of Two Semi-automated Forensic DNA Extraction Methods." Arab Journal of Forensic Sciences and Forensic Medicine 5, no. 2 (2023): 180–90. http://dx.doi.org/10.26735/yskr7711.

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Automation in forensic DNA analysis is crucial for analysts to reduce time, improve results, and decrease risk of contamination. With the variety of commercially available automated DNA extraction systems, comes the need for end-users to be informed of what they provide and what they might lack. Thus, this study aimed to evaluate the efficiency of two semi-automated DNA extraction systems used for forensic DNA analysis: Automate Express™ and Hamilton Microlab STAR™ system, for four parameters; reproducibility, stability, sensitivity and contamination. Overall, the results indicated that both s

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Côté, Annie, Manon Landry, Sara-Kim Rochette, Karine Gibson, Martine Lapointe, and Vahé Sarafian. "Automated DNA extraction from large volumes." Forensic Science International: Genetics Supplement Series 1, no. 1 (2008): 22–23. http://dx.doi.org/10.1016/j.fsigss.2007.10.216.

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Stangegaard, Michael, Benjamin B. Hjort, Thomas N. Hansen, Anders J. Hansen, and Niels Morling. "Automated extraction of DNA from clothing." Forensic Science International: Genetics Supplement Series 3, no. 1 (2011): e403-e404. http://dx.doi.org/10.1016/j.fsigss.2011.09.063.

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Brevnov, Maxim, Janna Mundt, Jacki Benfield, et al. "Automated Extraction of DNA from Forensic Sample Types Using the PrepFiler Automated Forensic DNA Extraction Kit." Journal of the Association for Laboratory Automation 14, no. 5 (2009): 294–302. http://dx.doi.org/10.1016/j.jala.2009.06.006.

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Liu, Andrew W., Alejandro Villar-Briones, Nicholas M. Luscombe, and Charles Plessy. "Automated phenol-chloroform extraction of high molecular weight genomic DNA for use in long-read single-molecule sequencing." F1000Research 11 (February 28, 2022): 240. http://dx.doi.org/10.12688/f1000research.109251.1.

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Background: Automation has increasingly become more commonplace in the research laboratory workspace. The introduction of articulated robotic arms allows the researcher more flexibility in the tasks a single piece of automated machinery can perform. We set out to incorporate automation in processing of genomic DNA organic extractions to increase throughput and limit researchers to the exposure of organic solvents. Methods: In order to automate the genome sequencing pipeline in our laboratory, we programmed a dual-arm anthropomorphic robot, the Robotic Biology Institute's Maholo LabDroid, to pe

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Williams, Sheri M., Cindy A. Meadows, and Elaine Lyon. "Automated DNA Extraction for Real-Time PCR." Clinical Chemistry 48, no. 9 (2002): 1629–30. http://dx.doi.org/10.1093/clinchem/48.9.1629.

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Guertler, Patrick, Adelina Eicheldinger, Paul Muschler, Ottmar Goerlich, and Ulrich Busch. "Automated DNA extraction from pollen in honey." Food Chemistry 149 (April 2014): 302–6. http://dx.doi.org/10.1016/j.foodchem.2013.10.129.

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Fujii, Koji, Shota Inokuchi, Tetsushi Kitayama, Hiroaki Nakahara, Natsuko Mizuno, and Kazumasa Sekiguchi. "DNA Extraction From Various Forensic Samples Using Automated Extraction Instruments." Japanese Journal of Forensic Science and Technology 17, no. 1 (2012): 15–26. http://dx.doi.org/10.3408/jafst.17.15.

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Bogožalec Košir, Alexandra, Dane Lužnik, Viktorija Tomič, and Mojca Milavec. "Evaluation of DNA Extraction Methods for Reliable Quantification of Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa." Biosensors 13, no. 4 (2023): 463. http://dx.doi.org/10.3390/bios13040463.

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Detection and quantification of DNA biomarkers relies heavily on the yield and quality of DNA obtained by extraction from different matrices. Although a large number of studies have compared the yields of different extraction methods, the repeatability and intermediate precision of these methods have been largely overlooked. In the present study, five extraction methods were evaluated, using digital PCR, to determine their efficiency in extracting DNA from three different Gram-negative bacteria in sputum samples. The performance of two automated methods (GXT NA and QuickPick genomic DNA extrac

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Cangi, Nídia, Valérie Pinarello, Laure Bournez, et al. "Efficient high-throughput molecular method to detect Ehrlichia ruminantium in ticks." Parasites & Vectors 10, no. 1 (2017): 566. https://doi.org/10.1186/s13071-017-2490-0.

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<strong>Background: </strong> <i>Ehrlichia ruminantium</i> is the causal agent of heartwater, a fatal tropical disease affecting ruminants with important economic impacts. This bacterium is transmitted by <i>Amblyomma</i> ticks and is present in sub-Saharan Africa, islands in the Indian Ocean and the Caribbean, where it represents a threat to the American mainland.<strong>Methods: </strong>An automated DNA extraction method was adapted for <i>Amblyomma</i> ticks and a new qPCR targeting the <i>pCS20</i> region was developed to improve <i>E. ruminantium</i> screening capacity and diagnosis. The

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Merel, P., B. Dupin, F. Comeau, L. Lacoste, and G. Vezon. "Completely automated extraction of DNA from whole blood." Clinical Chemistry 42, no. 8 (1996): 1285–86. http://dx.doi.org/10.1093/clinchem/42.8.1285.

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Meslier, Victoria, Elisa Menozzi, Aymeric David, et al. "Evaluation of an Adapted Semi-Automated DNA Extraction for Human Salivary Shotgun Metagenomics." Biomolecules 13, no. 10 (2023): 1505. http://dx.doi.org/10.3390/biom13101505.

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Recent attention has highlighted the importance of oral microbiota in human health and disease, e.g., in Parkinson’s disease, notably using shotgun metagenomics. One key aspect for efficient shotgun metagenomic analysis relies on optimal microbial sampling and DNA extraction, generally implementing commercial solutions developed to improve sample collection and preservation, and provide high DNA quality and quantity for downstream analysis. As metagenomic studies are today performed on a large number of samples, the next evolution to increase study throughput is with DNA extraction automation.

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Muscarella, Fabrizio, De Bonis, et al. "Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics." Cancers 11, no. 11 (2019): 1691. http://dx.doi.org/10.3390/cancers11111691.

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Thanks to personalized medicine trends and collaborations between industry, clinical research groups and regulatory agencies, next generation sequencing (NGS) is turning into a common practice faster than one could have originally expected. When considering clinical applications of NGS in oncology, a rapid workflow for DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue samples, as well as producing high quality library preparation, can be real challenges. Here we consider these targets and how applying effective automation technology to NGS workflows may help improve yield, tim

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Edberg, Andreas, Fredrik Aronsson, Eva Johansson, Elisabeth Wikander, Thomas Ahlqvist, and Hans Fredlund. "Endocervical swabs transported in first void urine as combined specimens in the detection of Mycoplasma genitalium by real-time PCR." Journal of Medical Microbiology 58, no. 1 (2009): 117–20. http://dx.doi.org/10.1099/jmm.0.003681-0.

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The aim of this study was to determine whether a patient's endocervical swab specimen can be transported in first void urine (FVU) as combined specimens for the detection of Mycoplasma genitalium by real-time PCR. The study also compared two different DNA extraction methods for observation of possible PCR inhibition. Three specimens, one endocervical swab specimen transported in 2-SP medium, one endocervical swab specimen transported in FVU and a FVU specimen, were collected from 329 women. All sample types underwent manual DNA extraction whereas in the DNA extraction study, 329 endocervical s

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Menu, Estelle, Jordi Landier, Elsa Prudent, Stéphane Ranque, and Coralie L’Ollivier. "Evaluation of 11 DNA Automated Extraction Protocols for the Detection of the 5 Mains Candida Species from Artificially Spiked Blood." Journal of Fungi 7, no. 3 (2021): 228. http://dx.doi.org/10.3390/jof7030228.

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The molecular detection of Candida plays an important role in the diagnosis of candidaemia, a major cause of morbidity and mortality. The sensitivity of this diagnosis is partly related to the efficiency of yeast DNA extraction. In this monocentric study, we investigated the suitability of 11 recent automated procedures for the extraction of low and high amounts of Candida DNA from spiked blood. The efficacy of the DNA extraction procedures to detect Candida spp. in blood samples ranged from 31.4% to 80.6%. The NucliSENSTM easyMAGTM procedure was the most efficient, for each species and each i

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Han, Sang-Ho, Ae-Ja Park, Ah-Reum Park, and Mun-Ho Ryu. "Automated DNA Extraction Monitoring System Based on MTConnect Technology." Applied Sciences 11, no. 2 (2021): 684. http://dx.doi.org/10.3390/app11020684.

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MTConnect standard technology provides simplicity, flexibility, and scalability in integrating various equipment and operating systems and enabling accurate and consistent data collection from any MTConnect-compatible system. Using MTConnect technology, it is possible to immediately identify the cause of a problem and respond quickly when a problem occurs. Molecular genetic diagnostic point-of-care testing (POCT) devices have received attention in recent years because they enable rapid disease diagnosis. A molecular genetic diagnostic POCT device is under development by the authors. The system

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Gruentzig, Alexander W., Catherine M. Klapperich, Andre Sharon, Jeff Braman, Anirban Chatterjee, and Alexis F. Sauer-Budge. "A new DNA extraction method for automated food analysis." Analytical Methods 3, no. 7 (2011): 1507. http://dx.doi.org/10.1039/c0ay00701c.

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Ordoñez, Elena, Laura Rueda, M. Paz Cañadas, Carme Fuster, and Vincenzo Cirigliano. "Evaluation of Sample Stability and Automated DNA Extraction for Fetal Sex Determination Using Cell-Free Fetal DNA in Maternal Plasma." BioMed Research International 2013 (2013): 1–4. http://dx.doi.org/10.1155/2013/195363.

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Objective. The detection of paternally inherited sequences in maternal plasma, such as the SRY gene for fetal sexing or RHD for fetal blood group genotyping, is becoming part of daily routine in diagnostic laboratories. Due to the low percentage of fetal DNA, it is crucial to ensure sample stability and the efficiency of DNA extraction. We evaluated blood stability at 4°C for at least 24 hours and automated DNA extraction, for fetal sex determination in maternal plasma.Methods. A total of 158 blood samples were collected, using EDTA-K tubes, from women in their 1st trimester of pregnancy. Samp

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Boucher St-Amour, Vincent-Thomas, Vipin Tomar, and François Belzile. "High-Throughput DNA Extraction Using Robotic Automation (RoboCTAB) for Large-Scale Genotyping." Plants 14, no. 15 (2025): 2263. https://doi.org/10.3390/plants14152263.

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Efficient and consistent DNA extraction is crucial for genotyping but often hindered by the limitations of traditional manual processes, which are labour-intensive, error-prone, and costly. We introduce a semi-automated, robotic-assisted DNA extraction (RoboCTAB) tailored for large-scale plant genotyping, leveraging advanced yet affordable liquid-handling robotic systems. The protocol/workflow integrates a CTAB extraction protocol specifically adapted for a robotic liquid-handling system, making it compatible with high-throughput genotyping techniques such as SNP genotyping and sequencing. Var

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Silgado, Aroa, Zaira Moure, Maykon T. de Oliveira, et al. "Comparison of DNA extraction methods and real-time PCR assays for the molecular diagnostics of chronic Chagas disease." Future Microbiology 15, no. 12 (2020): 1139–45. http://dx.doi.org/10.2217/fmb-2020-0023.

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Aim: This work aimed to compare the sensitivity of four protocols for the detection of Trypanosoma cruzi DNA in 98 blood samples from chronic Chagas disease patients. Materials & methods: Two DNA extraction (automated and manual) methods and two T. cruzi satellite DNA qPCRs (with a recent design and the usually used set of primers) were analyzed. Results: Both DNA extraction methods and qPCR assays tested in this work gave comparable qualitative results, although the lowest Ct values were obtained when samples were analyzed using the new set of primers for T. cruzi satellite DNA. Conclusio

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Al-Saud, Haya, Khaldoun Al-Romaih, Razan Bakheet, et al. "Automated SARS-COV-2 RNA extraction from patient nasopharyngeal samples using a modified DNA extraction kit for high throughput testing." Annals of Saudi Medicine 40, no. 5 (2020): 373–81. http://dx.doi.org/10.5144/0256-4947.2020.373.

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ABSTRACT BACKGROUND: The pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) has prompted a need for mass testing to identify patients with viral infection. The high demand has created a global bottleneck in testing capacity, which prompted us to modify available resources to extract viral RNA and perform reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) to detect SARS-COV-2. OBJECTIVES: Report on the use of a DNA extraction kit, after modifications, to extract viral RNA that could then be detected using an FDA-approved SARS-COV-2 RT-qPCR ass

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Patel, Shruti B., Jon Jurica, and Xiaoqing Hua. "Implementation of a high throughput automated platform for residual DNA quantitation." PLOS One 20, no. 4 (2025): e0322133. https://doi.org/10.1371/journal.pone.0322133.

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Host cell DNA is an impurity from cell-based manufacturing processes that must be controlled and monitored to ensure drug purity and safety. Conventional methods for measurement of residual host cell DNA in therapeutic protein require numerous preparations of plates, DNA extraction from the protein samples, followed by quantification of the extracted DNA using real-time PCR (qPCR). Preparation of plates for extraction is the most laborious step, including numerous manual steps such as sample dilution, standard curve preparation, as well as reagent and sample plate preparation. Additionally, mu

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Lee, Dongkyu, Deawook Kim, Jounghyuk Han, et al. "Integrated, Automated, Fast PCR System for Point-Of-Care Molecular Diagnosis of Bacterial Infection." Sensors 21, no. 2 (2021): 377. http://dx.doi.org/10.3390/s21020377.

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We developed an integrated PCR system that performs automated sample preparation and fast polymerase chain reaction (PCR) for application in point-of care (POC) testing. This system is assembled from inexpensive 3D-printing parts, off-the-shelf electronics and motors. Molecular detection requires a series of procedures including sample preparation, amplification, and fluorescence intensity analysis. The system can perform automated DNA sample preparation (extraction, separation and purification) in ≤5 min. The variance of the automated sample preparation was clearly lower than that achieved us

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Lee, Dongkyu, Deawook Kim, Jounghyuk Han, et al. "Integrated, Automated, Fast PCR System for Point-Of-Care Molecular Diagnosis of Bacterial Infection." Sensors 21, no. 2 (2021): 377. http://dx.doi.org/10.3390/s21020377.

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We developed an integrated PCR system that performs automated sample preparation and fast polymerase chain reaction (PCR) for application in point-of care (POC) testing. This system is assembled from inexpensive 3D-printing parts, off-the-shelf electronics and motors. Molecular detection requires a series of procedures including sample preparation, amplification, and fluorescence intensity analysis. The system can perform automated DNA sample preparation (extraction, separation and purification) in ≤5 min. The variance of the automated sample preparation was clearly lower than that achieved us

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Bekaert, Bram, Maarten H. D. Larmuseau, Maarten P. M. Vanhove, Anouschka Opdekamp, and Ronny Decorte. "Automated DNA extraction of single dog hairs without roots for mitochondrial DNA analysis." Forensic Science International: Genetics 6, no. 2 (2012): 277–81. http://dx.doi.org/10.1016/j.fsigen.2011.04.009.

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Becherer, Lisa, Jacob Friedrich Hess, Sieghard Frischmann, et al. "Point-of-Care System for HTLV-1 Proviral Load Quantification by Digital Mediator Displacement LAMP." Micromachines 12, no. 2 (2021): 159. http://dx.doi.org/10.3390/mi12020159.

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This paper presents a universal point-of-care system for fully automated quantification of human T-cell lymphotropic virus type 1 (HTLV-1) proviral load, including genomic RNA, based on digital reverse RNA transcription and c-DNA amplification by MD LAMP (mediator displacement loop-mediated isothermal amplification). A disposable microfluidic LabDisk with pre-stored reagents performs automated nucleic acid extraction, reaction setup, emulsification, reverse transcription, digital DNA amplification, and quantitative fluorogenic endpoint detection with universal reporter molecules. Automated nuc

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Khelaifia, Saber, Pierre-Yves Ramonet, Marielle Bedotto Buffet, and Michel Drancourt. "A semi-automated protocol for Archaea DNA extraction from stools." BMC Research Notes 6, no. 1 (2013): 186. http://dx.doi.org/10.1186/1756-0500-6-186.

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Montpetit, Shawn A., Ian T. Fitch, and Patrick T. O'Donnell. "A Simple Automated Instrument for DNA Extraction in Forensic Casework." Journal of Forensic Sciences 50, no. 3 (2005): 1–9. http://dx.doi.org/10.1520/jfs2004181.

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Willis, A., T. Morris, T. Hsiao, and C. Lind. "Assessment of automated cell-free DNA extraction utilizing Promega Maxwell." Human Immunology 85 (September 2024): 111078. http://dx.doi.org/10.1016/j.humimm.2024.111078.

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Chiodi, Claudia, Matteo Moro, Andrea Squartini, et al. "High-Throughput Isolation of Nucleic Acids from Soil." Soil Systems 4, no. 1 (2019): 3. http://dx.doi.org/10.3390/soilsystems4010003.

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DNA-based technologies have become widespread tools for soil microbiological analyses in recent years. DNA extraction from the soil is a key step for these approaches: it is a challenge for researchers as it is still both expensive and time-consuming when large surveys are planned. The aim of this study was to develop a high-throughput automated protocol for DNA extraction and purification from soil. The protocol was based on the BioSprint 96 platform and compared for validation with another automated procedure and two commercial column-based kits. To evaluate the performances of the protocols

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Lamia, Diouri, Uwiringiyeyezu Théophile, Abdelouahab Hinde, Malki Mohamed, Baibai Tarik, and Soukri Abdelaziz. "Comparison and Validation of Ichthyoplankton DNA Extraction Methods." Methods and Protocols 4, no. 4 (2021): 87. http://dx.doi.org/10.3390/mps4040087.

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Ichthyoplankton is the cluster of planktonic organisms that consists of fish eggs and larvae. These planktonic stages belong to the temporary zooplankton, representing future exploitable stocks. The study of the early ontogenesis of fish plays a key role in the understanding and evaluation of these populations through the study of their abundance and their spatio-temporal distribution. To better understand and protect these fisheries resources, it is essential to identify the different stages of fish embryonic development. This identification is usually performed using the classical method, ba

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Li, Xiaolan, Caroline J. Bosch-Tijhof, Xi Wei, et al. "Efficiency of chemical versus mechanical disruption methods of DNA extraction for the identification of oral Gram-positive and Gram-negative bacteria." Journal of International Medical Research 48, no. 5 (2020): 030006052092559. http://dx.doi.org/10.1177/0300060520925594.

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Objective Clinical diagnostics often requires the detection of multiple bacterial species in limited clinical samples with a single DNA extraction method. This study aimed to compare the bacterial DNA extraction efficiency of two lysis methods automated with the MagNA-Pure LC instrument. The samples included five oral bacterial species (three Gram-positive and two Gram-negative) with or without human saliva background. Methods Genomic DNA (gDNA) was extracted from bacterial cultures by bead-beating lysis (BMP) or chemical lysis (MP), followed by automated purification and measurement by quanti

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Rubinstein, Linda. "Comparing Two Automated Methods of DNA Extraction from Degraded Skeletal Remains." Forensic Medicine and Anatomy Research 09, no. 02 (2021): 24–30. http://dx.doi.org/10.4236/fmar.2021.92003.

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Mathot, Lucy, Monica Wallin, and Tobias Sjöblom. "Automated serial extraction of DNA and RNA from biobanked tissue specimens." BMC Biotechnology 13, no. 1 (2013): 66. http://dx.doi.org/10.1186/1472-6750-13-66.

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Hong, Seung-Bum, Yangseop Kim, and Jung-Hyun Park. "High-efficiency automated DNA extraction method for degraded old skeletal samples." Forensic Science International: Genetics Supplement Series 6 (December 2017): e365-e367. http://dx.doi.org/10.1016/j.fsigss.2017.09.108.

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Kim, Jungkyu, Michael Johnson, Parker Hill, Rahul S. Sonkul, Jongwon Kim, and Bruce K. Gale. "Automated microfluidic DNA/RNA extraction with both disposable and reusable components." Journal of Micromechanics and Microengineering 22, no. 1 (2011): 015007. http://dx.doi.org/10.1088/0960-1317/22/1/015007.

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Farani, Priscila S. G., Jacqueline Lopez, Amanda Faier-Pereira, Alejandro Marcel Hasslocher-Moreno, Igor C. Almeida, and Otacilio C. Moreira. "Utility of Magnetic Bead-Based Automated DNA Extraction to Improve Chagas Disease Molecular Diagnosis." International Journal of Molecular Sciences 26, no. 3 (2025): 937. https://doi.org/10.3390/ijms26030937.

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Chagas disease, caused by Trypanosoma cruzi, remains a significant global health challenge, particularly in the molecular diagnostics of low parasitemia during the chronic phase. This highlights the critical need for enhanced diagnostic methodologies. In response, this study evaluates the effectiveness of an automated magnetic beads-based DNA extraction method in improving the molecular diagnosis of Chagas disease compared to the traditional silica column-based extraction. Accordingly, this research seeks to enhance the DNA yield, purity, and sensitivity of real-time PCR (qPCR) assays for dete

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Polatoglou, Eleni, Zsuzsanna Mayer, Vida Ungerer, Abel J. Bronkhorst, and Stefan Holdenrieder. "Isolation and Quantification of Plasma Cell-Free DNA Using Different Manual and Automated Methods." Diagnostics 12, no. 10 (2022): 2550. http://dx.doi.org/10.3390/diagnostics12102550.

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Plasma cell-free DNA (cfDNA) originates from various tissues and cell types and can enable minimally invasive diagnosis, treatment and monitoring of cancer and other diseases. Proper extraction of cfDNA is critical to obtain optimal yields and purity. The goal of this study was to compare the performance of six commercial cfDNA kits to extract pure, high-quality cfDNA from human plasma samples and evaluate the quantity and size profiles of cfDNA extracts—among them, two spin-column based, three magnetic bead-based and two automatic magnetic bead-based methods. Significant differences were obse

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Vidergar, Nina, Nataša Toplak, and Matjaž Kuntner. "Streamlining DNA Barcoding Protocols: Automated DNA Extraction and a New cox1 Primer in Arachnid Systematics." PLoS ONE 9, no. 11 (2014): e113030. http://dx.doi.org/10.1371/journal.pone.0113030.

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Gehrig, C., D. Kummer, and V. Castella. "Automated DNA extraction using the QIAsymphony platform: Estimation of DNA recovery from simulated forensic stains." Forensic Science International: Genetics Supplement Series 2, no. 1 (2009): 85–86. http://dx.doi.org/10.1016/j.fsigss.2009.07.009.

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Smit, Maarten L., Belinda A. J. Giesendorf, Jacqueline A. M. Vet, Frans J. M. Trijbels, and Henk J. Blom. "Semiautomated DNA Mutation Analysis Using a Robotic Workstation and Molecular Beacons." Clinical Chemistry 47, no. 4 (2001): 739–44. http://dx.doi.org/10.1093/clinchem/47.4.739.

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Abstract Background: Our increasing knowledge of the genetic basis of inheritable diseases requires the development of automated reliable methods for high-throughput analyses. Methods: We investigated the combination of semiautomated DNA extraction from blood using a robotic workstation, followed by automated mutation detection using highly specific fluorescent DNA probes, so-called molecular beacons, which can discriminate between alleles with as little as one single-base mutation. We designed two molecular beacons, one recognizing the wild-type allele and the other the mutant allele, to dete

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Stan, Emanuela, Camelia-Oana Muresan, Raluca Dumache, et al. "From Jane Doe to Sofia: DNA Extraction Protocol from Bones and Teeth without Liquid Nitrogen for Identifying Skeletal Remains." International Journal of Molecular Sciences 25, no. 10 (2024): 5114. http://dx.doi.org/10.3390/ijms25105114.

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DNA analysis plays a crucial role in forensic investigations, helping in criminal cases, missing persons inquiries, and archaeological research. This study focuses on the DNA concentration in different skeletal elements to improve human identification efforts. Ten cases of unidentified skeletal remains brought to the Institute of Forensic Medicine in Timisoara, Romania, underwent DNA analysis between 2019 and 2023. The results showed that teeth are the best source for DNA extraction as they contain the highest concentration of genetic material, at 3.68 ng/µL, compared to the petrous temporal b

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Harper, Kathryn A., Kelly A. Meiklejohn, Richard T. Merritt, Jessica Walker, Constance L. Fisher, and James M. Robertson. "Isolation of Mitochondrial DNA from Single, Short Hairs without Roots Using Pressure Cycling Technology." SLAS TECHNOLOGY: Translating Life Sciences Innovation 23, no. 1 (2017): 97–105. http://dx.doi.org/10.1177/2472630317732073.

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Hairs are commonly submitted as evidence to forensic laboratories, but standard nuclear DNA analysis is not always possible. Mitochondria (mt) provide another source of genetic material; however, manual isolation is laborious. In a proof-of-concept study, we assessed pressure cycling technology (PCT; an automated approach that subjects samples to varying cycles of high and low pressure) for extracting mtDNA from single, short hairs without roots. Using three microscopically similar donors, we determined the ideal PCT conditions and compared those yields to those obtained using the traditional

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Kil, Byeong-Heon, Ji-Seong Park, Mun-Ho Ryu, Chan-Young Park, Yu-Seop Kim, and Jong-Dae Kim. "Cloud-Based Software Architecture for Fully Automated Point-of-Care Molecular Diagnostic Device." Sensors 21, no. 21 (2021): 6980. http://dx.doi.org/10.3390/s21216980.

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This paper proposes a cloud-based software architecture for fully automated point-of-care molecular diagnostic devices. The target system operates a cartridge consisting of an extraction body for DNA extraction and a PCR chip for amplification and fluorescence detection. To facilitate control and monitoring via the cloud, a socket server was employed for fundamental molecular diagnostic functions such as DNA extraction, amplification, and fluorescence detection. The user interface for experimental control and monitoring was constructed with the RESTful application programming interface, allowi

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Zhuang, Bin, Junping Han, Guangxin Xiang, et al. "A fully integrated and automated microsystem for rapid pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms." Lab on a Chip 16, no. 1 (2016): 86–95. http://dx.doi.org/10.1039/c5lc01094b.

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Stangegaard, Michael, Claus Børsting, Laura Ferrero-Miliani, et al. "Evaluation of Four Automated Protocols for Extraction of DNA from FTA Cards." Journal of Laboratory Automation 18, no. 5 (2013): 404–10. http://dx.doi.org/10.1177/2211068213484472.

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Taylor, Sheryle A., Angela O. W. Tsang, Kin H. Fu, Dagmar K. Kalousek, and Douglas E. Horsman. "Comparative Study of Automated Versus Manual Extraction of DNA from Clinical Specimens." American Journal of Clinical Pathology 93, no. 6 (1990): 749–53. http://dx.doi.org/10.1093/ajcp/93.6.749.

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Zupanič Pajnič, Irena, Magdalena Debska, Barbara Gornjak Pogorelc, et al. "Highly efficient automated extraction of DNA from old and contemporary skeletal remains." Journal of Forensic and Legal Medicine 37 (January 2016): 78–86. http://dx.doi.org/10.1016/j.jflm.2015.11.001.

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Nourrisson, Céline, Julie Brunet, Pierre Flori, et al. "Comparison of DNA Extraction Methods and Real-Time PCR Assays for the Detection of Blastocystis sp. in Stool Specimens." Microorganisms 8, no. 11 (2020): 1768. http://dx.doi.org/10.3390/microorganisms8111768.

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Abstract:

Diagnosis of Blastocystis in stool may be challenging, as microscopic examination and culture-based methods have demonstrated low sensitivity. Molecular detection assays are now available for this enteric parasite, based on “in-house” or commercial-developed techniques. The aim of this study was to assess and compare the performance of (i) two DNA extraction methods (manual versus automated), and (ii) four qPCR assays (three “in-house” and one commercialized), for detection of Blastocystis sp. in human stools. One hundred and forty stools were included, among which 76 were confirmed to be posi

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FRANCEZ, PABLO ABDON DA COSTA, CIRO AUGUSTO FERNANDES DE OLIVEIRA PENIDO, GILCELENE DO SOCORRO MEDEIROS DE BRITO COSTA, et al. "Comparison between automated DNA extraction employing the EZ1 platform and manual methods using real forensic samples." Revista Brasileira de Criminalística 10, no. 1 (2021): 44–56. http://dx.doi.org/10.15260/rbc.v10i1.476.

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In the last 30 years, advances in Molecular Biology techniques have allowed its application as an essential tool in the criminal investigation, with a particularly relevant application in the determination of authorship of crimes and the identification of missing persons and unknown corpses. However, with the popularization of Forensic Genetics and the growth of violence, there has been a significant increase in the demand for these tests, which has led Forensic Genetics Laboratories to search for more efficient and safe methods of processing this increasing volume of samples. In this sense, a

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