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Relevant bibliographies by topics / Azotobacter. Nitrogen

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Academic literature on the topic 'Azotobacter. Nitrogen'

Author: Grafiati

Published: 4 June 2021

Last updated: 14 February 2022

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Journal articles on the topic "Azotobacter. Nitrogen"

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Hindersah, Reginawanti, Nadia Nuraniya Kamaluddin, Suman Samanta, Saon Banerjee, and Sarita Sarkar. "Role and perspective of Azotobacter in crops production." SAINS TANAH - Journal of Soil Science and Agroclimatology 17, no. 2 (December 30, 2020): 170. http://dx.doi.org/10.20961/stjssa.v17i2.45130.

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<p>Low nitrogen content in soil is usually overcome by chemical fertilization. After long application period, high-dose and intensive use of N fertilizers can cause ammonia volatilization and nitrates accumulation in soil. In sustainable agriculture, the use of bacterial inoculant integrated with nutrient management system has a role in soil health and productivity. Azotobacter-based biofertilizer is suggested as a chemical nitrogen fertilizer substitute or addition in crop production to improve available nutrients in the soil, provide some metabolites during plant growth, and minimize fertilizer doses. The objective of this literature reviewed paper is to discuss the role of Azotobacter in agriculture; and the prospective of Azotobacter to increase yield and substitute the chemical fertilizer in food crops production. The results revealed that mechanisms by Azotobacter in plant growth enhancement are as biofertilizer, biostimulant, and bioprotectant. Nitrogen fixation by Azotobacter is the mechanism to provide available nitrogen for uptake by roots. Azotobacter stimulates plant growth through phytohormones synthesis; indole acetic acid, cytokinins, and gibberellins are detected in the liquid culture of Azotobacter. An indirect effect of Azotobacter is exopolysaccharide production and plant protection. Inoculation of Azotobacter in the field integrated with organic matter and reduced chemical fertilizer are reported to improve plant growth and yield.</p>

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Hindersah, Reginawanti, Marthin Kalay, Abraham Talahaturuson, and Yansen Lakburlawal. "NITROGEN FIXING BACTERIA AZOTOBACTER AS BIOFERTILIZER AND BIOCONTROL IN LONG BEAN." Agric 30, no. 1 (July 31, 2018): 25–32. http://dx.doi.org/10.24246/agric.2018.v30.i1.p25-32.

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Azotobacter is Plant Growth Promoting Rhizobacteria through the mechanism of nitrogen fixation and phytohormon production but this rhizobacteria has a role to control plant diseases. The objective of experiment was to evaluate the activity of Azotobacter as biofertilizers as well as biocontrol on long bean cultivation in damping off endemic land in Ambon city, Maluku Province. The field experiment was arranged in completely randomized block design. Inoculation of long bean by Azotobacter has been done by seed inoculation, soil inoculation before planting, and plant inoculation. Plants treated with Azotobacter received fertilizer NPK of ¾ or ½ dosage recommendation while control plants were received 100% NPK. Research showed that no differences between yield of long bean inoculated with Azotobacter sp +reduced doses of NPK with that of control plants. Any application method of Azotobacter inoculation lowered damping off diseases incidence significantly until 10 days after planting, but no effect of inoculation on late blight at 21 days after planting. This study confirmed that Azotobacter has dual activity to reduce the dose of NPK fertilizer and control damping off.

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Bhattarai, Dhruba R., Krishna P. Poudyal, and Shanta Pokhrel. "Effect of Azotobacter and Nitrogen Levels on Fruit Yield and Quality of Bell Pepper." Nepal Journal of Science and Technology 12 (July 21, 2012): 29–34. http://dx.doi.org/10.3126/njst.v12i0.6475.

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Inorganic fertilizers alone cannot sustain high levels of productivity. It may cause deterioration of soil environment leading to low productivity. An investigation was carried out to develop integrated nutrient management technology for fruit yield of bell pepper (Capsicum annuum L.). The experiment was conducted at Horticulture Research Division, Nepal Agricultural Research Council Khumaltar, Lalitpur during 2010/011. The experiment was laid out in a randomized block design with three replications and having seven treatments viz., Control (100% NPK), Azotobacter + 25% N + PK, Azotobacter + 50% N + PK, Azotobacter + 75% N + PK, Azotobacter + FYM + 25% N + PK, Azotobacter + FYM + 50% N + PK, Azotobacter + FYM + 75% N + PK. Among the tested treatments, T7 (Azotobacter + FYM + 75 % N + PK) recorded the maximum plant height (66.18 cm), number of primary branches (8.20) and fruit yield (18.08 kg) per plot.DOI: http://dx.doi.org/10.3126/njst.v12i0.6475 Nepal Journal of Science and Technology 12 (2011) 29-34

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Das, A. C., and D. Saha. "Influence of diazotrophic inoculations on nitrogen nutrition of rice." Soil Research 41, no. 8 (2003): 1543. http://dx.doi.org/10.1071/sr03115.

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An experiment was conducted in microplots (7 by 7m) to investigate the effect of 2 non-symbiotic N2-fixing bacteria [Azotobacter (strain CS1) and Azospirillum (strain CM4)] in the presence of 50 kg N/ha on the performances of the diazotrophs with respect to nitrogen accretion and its transformation in the rhizosphere soils of rice (Oryza sativa L. cv. IR-36). In most cases, a successful inoculation of the diazotrophs was recorded, with the proliferation of Azotobacter and Azospirillum, either alone or in combination, in the rhizosphere soils, and nitrogenase activity (C2H2 reduction) of the microbes was present in rice roots. The uninoculated soil receiving 100 kg N/ha recorded the highest amount of total nitrogen, non-hydrolysable organic nitrogen, available nitrogen, and hydrolysable organic nitrogen content in the rhizosphere soils, resulting in greater yield of the crop. Inoculation of the diazotrophs substantially increased different fractions of nitrogen content in the rhizosphere soils, and the increase in total nitrogen, non-hydrolysable organic nitrogen, and hydrolysable organic nitrogen was greater due to Azotobacter than either Azospirillum or a combination of Azotobacter and Azospirillum. Total and mineral nitrogen content increased at maximum tillering to flowering stages of the crop, followed by a decline at maturity, whereas, hydrolysable organic nitrogen decreased with a concomitant increase in non-hydrolysable fraction with the age of the crop.

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Shawky, B. T., Y. Ghali, F. A. Ahmed, and T. Kahil. "Ammonium-nitrogen metabolism and nitrogen fixation in azotobacter vinelandii." Acta Biotechnologica 7, no. 6 (1987): 555–62. http://dx.doi.org/10.1002/abio.370070617.

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Wahyuni, Mardiana, Agus Triani, and Mariani Sembiring. "Pengaruh Kompos Mucuna bracteata Dan Azotobacter Terhadap Pertumbuhan Dan Kadar Nitrogen Bibit Kelapa Sawit." Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian 4, no. 2 (June 29, 2020): 119–27. http://dx.doi.org/10.31289/agr.v4i2.3735.

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Through the nursery process with certain treatments can be produced quality seeds. Application of Mucuna bracteata compost and Azotobacter on growing media in polybags is one alternative that expected to increase plant growth. This study aimed to determine the effect of M. bracteata compost and Azotobacter and its combination on growth and nitrogen levels of oil palm seedlings. This research was conducted at STIPAP Medan in December 2018 - June 2019 with a Factorial Randomized Block Design (RBD). Treatment compost (K) 3 levels ie K0, K1 and K2 and Azotobacter (A) with 3 levels are A0, A1 and A2. M. bracteata treatment had a very significant effect on seedling height, number of leaves, shoot dry weight and root dry weight. Azotobacter treatment did not significantly affect the parameters of observation, increasing levels of leaf N 13-15%. Interaction compost and Azotobacter treatment had no significant effect. The best treatment is the K2A2 of 1.2 kg of compost and 30 ml of Azotobacter each oil palm seedlings planted in the polybag.

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Hindersah, Reginawanti, Neni Rostini, Arief Harsono, and Dan Nuryani. "Peningkatan Populasi, Pertumbuhan dan Serapan Nitrogen Tanaman Kedelai dengan Pemberian Azotobacter Penghasil Eksopolisakarida." Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) 45, no. 1 (May 4, 2017): 30–35. http://dx.doi.org/10.24831/jai.v45i1.13801.

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Nitrogen-fixing Azotobacter is widely used as biofertilizer in sustainable agriculture. The bacteria produce exopolysaccharide which might have a significant role in enhancing soybean nitrogen uptake and growth. The objective of this research was to obtain growth media of Exopolysaccharide–producing Azotobacter; and increase shoot and root growth as well as nitrogen uptake of soybean var. Anjasmoro at early vegetative phase following inoculation of Azotobacter chroococcum liquid. Research consist of two phase, 1) determination of organic-based media for A. chroococcum liquid inoculant production, and 2) pot experiment for application of liquid inoculant on soybean. The first experiment was performed in a series of batch fermenter consisted of several organic media for 72 hours. The second experiment was set in completely randomized design consisted of three density of liquid inoculant. The results verified that the best media which induced exopolysachharide production of A. chroococcum was 1% molase enriched with 0.1% NH4Cl. Liquid inoculant clearly enhanced population of Azotobacter in soybean rhizosphere, plant height, roots dry weight and N uptake of 21 day old soybean. This research implied that A. chroococcum might be used as biofertilizer at early growth of soybean. Keywords: Azotobacter chroococcum, biofertilizer, liquid inoculat

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Saffeullah, Peer, Neelofer Nabi, and Shahid Umar. "Influence of Integrated Approach of Azotobacter and Nitrogen Fertilizer on Various Morpho-Physiological and Biochemical Parameters of Brassica Oleracea L. var. capitata." Current World Environment Special Issue, no. 1 (June 16, 2021): 68–77. http://dx.doi.org/10.12944/cwe.16.special-issue1.06.

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This study was formulated to evaluate the impact of bacterization with Azotobacter of two cabbage genotypes (Pusa Early golden acre and Pusa drum head) under field conditions. The study was performed in herbal garden of Jamia Hamdard, New Delhi. The plants were treated with graded treatments (60,120,180 kg N ha-1) of N alone and in combination with seedling inoculation withAzotobacter. The plants were sampled and tested for various morpho-physiological and biochemical parameters. Chlorophyll content, NR activity, protein content, sugar content and phenol content was found to be significantly higher in plants treated with N in combination in Azotobacter. Thus, the use of Azotobacteras a supplement or biofertilizer in integrated nutrient management systems was highly recommended to minimize the application rates of synthetic fertilizers and attain the goal of sustainable agriculture.

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Jain, Devendra, Jyoti Sharma, Gunnjeet Kaur, Ali Asger Bhojiya, Surya Chauhan, Vimal Sharma, Archna Suman, Santosh Ranjan Mohanty, and Elina Maharjan. "Phenetic and Molecular Diversity of Nitrogen Fixating Plant Growth Promoting Azotobacter Isolated from Semiarid Regions of India." BioMed Research International 2021 (January 9, 2021): 1–9. http://dx.doi.org/10.1155/2021/6686283.

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In the present study, 24 Azotobacter strains were isolated from soils of different areas of southern Rajasthan and characterized at biochemical, functional, and molecular levels. The isolated Azotobacter strains were gram negative and cyst forming when viewed under the microscope. These strains were also screened for their plant growth promoting activities and the ability of these isolates to survive under abiotic stress conditions viz. salt, pH, temperature, and drought stress. All the isolates showed IAA, siderophore, HCN, and ammonia production, whereas seven Azotobacter strains showed phosphate solubilization. Amplified Ribosomal DNA Restriction Analysis (ARDRA) revealed significant diversity among Azotobacter strains and the dendrogram obtained differentiated twenty-four of the strains into two major clusters at a similarity coefficient of 0.64. Qualitative and quantitative N2 fixation abilities of these strains were also detrained, and the amounts of acetylene reduced by Azotobacter strains were in the range of 1.31 to 846.56 nmol C2H4 mg protein−1 h−1. The strains showing high nitrogen fixation ability with multiple PGP activities were selected for further pot studies, and these Azotobacter strains significantly increased the various plant growth parameters of maize plantlets. Furthermore, the best Azotobacter isolates were subjected to 16S rRNA sequencing and confirmed their identities as Azotobacter sp. The indigenous Azotobacter strains with multiple PGP activities could be further used for commercial production.

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Hindersah, Reginawanti, Gina Nurhabibah, Priyanka Asmiran, and Etty Pratiwi. "Antibiotic Resistance of Azotobacter Isolated from Mercury-Contaminated Area." Journal of Agricultural Studies 7, no. 2 (August 1, 2019): 70. http://dx.doi.org/10.5296/jas.v7i3.14834.

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Nitrogen-fixing Azotobacter is a renewable source of biofertilizer for plant growth. Increased of antibiotic level in soil due to intensive used manure is believed to induce bacterial sensitivity to antaibiotic. An antibiotic sensitivity test has been carried out to study the inhibition effect of ampicillin, streptomycin, tetracycline and chloramphenicol on Azotobacter isolated from mercury-contaminated taling. The resistance test was performend by using disc plate method in Nitrogen-free Ashby’s agar with and without mercury. The results showed that the presence of 20 mg/L mercury in plate agar totally inhibited Azotobater growth. In the absence of mercury chloride, all isolates showed different sensitivity to antibiotics. Growth of Azotobacter buru1 was only inhibited by tetracycline. Azotobacter buru2 was susceptible to high and low concentration of tetracycline and streptomycin but they were resistance to low concentration of chloramphenicol as well as ampicillin; while Azotobacter bd3a were sensitive to all tested antibiotic. In conclusion, order of Azotobacter resistance to antibiotics in the absence of mercury was Bd3a<Buru2<Buru1. This research have not revealed the resistance of Azotobacter to antibiotic in the presence of mercury.

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Dissertations / Theses on the topic "Azotobacter. Nitrogen"

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Sarıbay, Gül Fidan. "Growth and nitrogen fixation dynamic of azotobacter chroococcum in nitrogen-free and omw containing medium." Ankara : METU, 2003. http://etd.lib.metu.edu.tr/upload/1098961/index.pdf.

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Kahindi, James H. P. "Efficiency of nitrogen fixation in Azotobacter chroococcum." Thesis, University of Sussex, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358929.

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Wilson, Mark Steven Michael. "Mutagenesis of nifE and nifN from Azotobacter vinelandii." Thesis, Virginia Tech, 1988. http://hdl.handle.net/10919/43071.

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The products of nifE and nifN from Azotobacter vinelandii, which are involved in the biosynthesis of the iron-molybdenum cofactor (FeMo-co) co) from nitrogenase, have been analyzed using a variety of mutagenic techniques. NifE was the object of several site-specific, amino acid substitutions that were designed to elicit information regarding metal cluster ligands, subunit-subunit interactions, and the proposed transfer of FeMo-co.from a nifEN-products complex to the apo-MoFe protein. A model of metal cluster binding; regions within the nifEN-products is discussed insofar as it relates to the rationale for the targeting of particular amino acids for-substitution. A translational fusion between nifN and lacZ was constructed and used to study the regulation of nifEN. This gene fusion was regulated in the same manner as wild type nifN and produced a fusion protein which was enzymatically active with respect to substrates of Î²-galactosidase. Results from mutant strains which carry lesions in nifH or nifA in addition to the nifN::lacZ fusion are presented and discussed.
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Brewin, Brett. "Mechanism of ammonium excretion in NifL mutants of Azotobacter vinelandii." Thesis, University of East Anglia, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323244.

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Saribay, Gul Fidan. "Growth And Nitrogen Fixation Dynamics Of Azotobacter Chroococcum In Nitrogen-free And Omw Containing Medium." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/1098961/index.pdf.

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Olive Mill Wastewater (OMW), by-product of oil industry, is a dark liquid with a characteristic fetid smell, bitter taste and bright appearance
having a high pollution potential, creating serious problems in countries producing olive oil. Azotobacter chroococcum as a Nitrogen-fixing bacteria can bioremediate OMW, by degrading its toxic constituents. With the help of this detoxification process OMW can be used as biofertilizer. In this study, the dynamics of growth and nitrogen fixation at different physiological conditions and nutrient requirements of A. chroococcum in chemically defined N-free medium was determined. These parameters were cultivation conditions such as pH, temperature and aeration and some additives such as inorganic salts, boric acid and nitrogen. Consequently, the maximum cell concentration were obtained when A. chroococcum was grown at neutral pH, 35&
#61616
C, 150 rpm and in medium supplemented with manganese salt at 0.01% concentration. The maximum nitrogen fixation products were attained when A. chroococcum was grown under the same conditions except at pH 8. Further, bioremediation of OMW by A. chroococcum was examined. When A. chroococcum was cultivated in OMW containing basal medium at 10% OMW concentration, a cell density 12 times higher than in the OMW free medium was achieved. Also, it was found to have maximum increase in extracellular protein concentration (112 mg/l) at 10% OMW containing medium and maximum increase in ammonia concentration (9.05 mg/l) at 5% OMW containing medium.

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Kim, ChulHwan. "Azotobacter vinelandii nitrogenase : role of the MoFe protein [alpha]-subunit histidine-195 residue in catalysis /." This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-06062008-164937/.

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Burger, Eva-Maria Michaela [Verfasser], and Oliver [Akademischer Betreuer] Einsle. "Structural characterisation of nitrogen fixation by the enzyme nitrogenase of Azotobacter vinelandii." Freiburg : Universität, 2015. http://d-nb.info/1119805805/34.

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Setterquist, Robert Alan. "Site-directed mutagenesis of the nitrogenase MoFe protein from Azotobacter vinelandii." Thesis, Virginia Polytechnic Institute and State University, 1989. http://hdl.handle.net/10919/50091.

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A model describing the potential amino acid ligands to the four 4Fe-4S centers (P-clusters) within the Azotobacter vinelandii nitrogenase MoFe protein is presented. Based on interspecies and intersubunit amino acid comparisons of the α- and ß-subunits of the MoFe protein, and the FeMoco biosynthetic proteins, NifE and NifN, four conserved residues (Cys62, His83, Cys88, Cys154 all proposed P-cluster ligands) within the α- subunit were targeted for site-directed mutagencsis studies. In order to define a range of acceptable substitutions, 35 specific site-mutants have been constructed, each with a different amino acid replacement at one of the four targeted positions. Previous studies indicated that these residues were important for MoFe activity, and may act as metallocenter ligands. Unusual redox and spectroscopic properties of the Fe-S centers suggest the involvement of ligands other than the four typical cysteines, though extrusion requirements indicate that some thiol ligands are likely. Surprisingly, mutants with an Asp, Gly, Thr, or Ser substituted for Cys88 are still capable of diazotrophic growth (Nif+), though whole cell and crude extract acetylene reduction activity is lowered. Several substitutions (Cys, Asp, Phe, Asn, Met, Tyr, Leu) are tolerated at the His83 position, these Nif+ mutant strains also have varying acetylene reduction rates and growth rates. All mutants with substitutions at positions 62, 154, resulted in complete loss of diazotrophic growth. The results could be interpreted by the following explanations: 1) Our proposed model for the P-cluster ligation within the MoFe protein is incorrect. 2) Some substitutions permit P-cluster rearrangement to a semi-functional state. 3) Either, P-clusters are not absolutely essential for diazotrophic growth, or the enzyme can function with a reduced number of these metal centers.
Master of Science
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Jacob, Jansen Philip. "Specificity and regulatory properties of the transcriptional activators VnfA and AnfA of Azotobacter vinelandii." Thesis, University of Sussex, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259964.

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Sippel, Daniel [Verfasser], and Oliver [Akademischer Betreuer] Einsle. "Structure of the Vanadium Nitrogenase of Azotobacter vinelandii and mechanistic insights into biological nitrogen fixation." Freiburg : Universität, 2017. http://d-nb.info/1166559327/34.

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Book chapters on the topic "Azotobacter. Nitrogen"

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Eady, R. R., R. Pau, D. J. Lowe, and F. J. Luque. "Vanadium nitrogenase of Azotobacter." In Nitrogen Fixation, 125–33. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4684-6432-0_14.

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Blanco, G., F. Ramos, J. R. Medina, J. C. Gutierrez, and Ma Tortolero. "A Chromosomal Linkage Map of Azotobacter Vinelandii." In Nitrogen Fixation, 59–60. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3486-6_9.

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Oelze, J. "Diazotrophic mixed culture of Azotobacter vinelandii and Rhodobacter capsulatus." In Nitrogen Fixation, 509–12. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3486-6_103.

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Sarić, M., Sarić Zora, and B. Krstić. "Specific Responses of Azotobacter Strains and Sugar Beet Genotypes." In Nitrogen Fixation, 333–35. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3486-6_69.

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Bishop, Paul E., Sheela I. MacDougal, Elizabeth D. Wolfinger, and Casendra L. Shermer. "Genetics of alternative nitrogen fixation systems in Azotobacter vinelandii." In Nitrogen Fixation, 789–95. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4684-6432-0_67.

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Poza-Carrión, César, Carlos Echavarri-Erasun, and Luis M. Rubio. "Regulation of nif Gene Expression in Azotobacter vinelandii." In Biological Nitrogen Fixation, 99–108. Hoboken, NJ, USA: John Wiley & Sons, Inc, 2015. http://dx.doi.org/10.1002/9781119053095.ch9.

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Georgiadis, Millie M., Pinak Chakrabarti, and Douglas C. Rees. "Crystal structure of the nitrogenase iron protein from Azotobacter vinelandii." In Nitrogen Fixation, 111–16. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4684-6432-0_12.

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El-Mokadem, T. M., O. H. El-sayed, Y. M. Ahmad, and M. Hassan. "Xylanase Production by Transformed Azotobacter." In Biological Nitrogen Fixation for the 21st Century, 486. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5159-7_301.

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Kennedy, Christina, Anil Bali, Gonzalo Blanco, Asunscion Contreras, Martin Drummond, Mike Merrick, Jean Walmsley, and Paul Woodley. "Regulation of Expression of Genes for Three Nitrogenases in Azotobacter Vinelandii." In Nitrogen Fixation, 13–23. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3486-6_2.

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Miclaus, N., C. Vannini, E. Gregori, G. Chini-Zittelli, and P. Coianiz. "Characterization of a Mutant of Azotobacter Chroococcum Resistant to some Fungicides." In Nitrogen Fixation, 57–58. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3486-6_8.

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Conference papers on the topic "Azotobacter. Nitrogen"

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HINDERSAH, REGINAWANTI. "Pertumbuhan dan komposisi eksopolisakarida bakteri pemfiksasi nitrogen Azotobacter spp. pada media yang mengandung kadmium." In Seminar Nasional Masyarakat Biodiversitas Indonesia. Masyarakat Biodiversitas Indonesia, 2015. http://dx.doi.org/10.13057/psnmbi/m010718.

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Breica Borozan, Aurica, Despina-Maria Bordean, Gabriel Bujanca, Delia Dumbrava, and Sorina Popescu. "CONTROL OF PLANTS OF LOTUS CORNICULATUS L. ON AEROBIC AND ANAEROBIC FREE NITROGEN-FIXING BACTERIA." In GEOLINKS International Conference. SAIMA Consult Ltd, 2020. http://dx.doi.org/10.32008/geolinks2020/b1/v2/07.

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The free nitrogen fixing bacteria are able to mobilize important soil nutrients, transforming through biological processes the unusable molecular nitrogen into an active form and to improve soil fertility, influence many aspects of plant health and ensure their growth, showing interest for the scientific world and farmers. But, on the other hand, this bacterial segment may be influenced by the edaphic factors and the interconnection with the plants, the growth phase, the physiological state and the root system of the plant, by the root exudates, which demonstrates the importance of the bacterial community monitoring from the area of plants influence throughout the growing periods The aim of this study was to evaluate the influence of the age of the plants used as biofertilizer and soil moisture on the free nitrogen fixing bacterial communities (the genera Azotobacter and Clostridium) associated with the roots of the perennial plants of Lotus corniculatus L. There were two zones of interest, namely the area of influence of the roots of the plants (rhizosphere) but also the more distant area (edaphosphere). For the study of aerobic and anaerobic free nitrogen fixing bacteria soil samples were taken together with adjacent plants of Lotus corniculatus L. The experimental variants were located in the western part of Romania, the plants being cultivated on the same soil type, but on different plots, that were in the I-IV years of culture. The influence of Lotus corniculatus L. plants on the free nitrogen fixing bacteria has been reported in control experimental variants. Isolation and study of this bacterial group from the 8 experimental variants was performed on a specific mineral medium, favorable for the growth of the two bacterial genera. The results were evaluated after 5 and 10 days of incubation. Between the two assesments there were no noticeable differences in the nitrogen fixing bacterial community, except for the stimulatory effect observed in the control vatiant and rhizosphere of the first year culture. The plants influence on aerobic and anaerobic free nitrogen fixing bacteria was obvious in the II and IV years of the Lotus corniculatus L. culture, compared to the 76 control variants and varies substantially depending on the age of the plant. In most analyzed soil samples, both bacterial genera, Azotobacter and Clostridium were present, confirming the known ecological relation of unilateral advantage or passive stimulation of the aerobic bacteria compared to the anaerobic clostridia. Exceptions were the samples from the cultures of the first year (rhizosphere and control), but also the rhizosphere from the culture of the year II, where only anaerobic nitrogen fixing bacteria were detected. Our results suggested that plant-soil interactions exert control over the bacteria being studied.

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Melnichuk, T. N., A. A. Gongalo, A. Yu Egovtseva, E. R. Abdurashytova, and E. N. Turin. "Impact of microbial preparations on the activity of rhizosphere and the productivity of oil flax under no-till." In РАЦИОНАЛЬНОЕ ИСПОЛЬЗОВАНИЕ ПРИРОДНЫХ РЕСУРСОВ В АГРОЦЕНОЗАХ. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-15.05.2020.16.

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Microbial preparations improve mineral nutrition of plants, protect against phytopathogens, and increase their resistance to stress factors. The aim of our research is to study the effect of microbial preparations on the biological activity of rhizosphere and the productivity of oil flax under no-till in the Crimean Steppe. Microbiological analysis of the rhizosphere of oil flax showed that there is a tendency to increase the number of microorganisms of various ecological and trophic groups both under the conditions of the conventional farming system (CFS) and no-till when seeds are inoculated with a complex of microbial preparations (CMP). Under CFS, the number of microorganisms using mineral forms of nitrogen as nutrition increased by 28 %; pedotrophs – by 37 %; ammonifiers and oligotrophs increased under both farming systems. The total number of nitrogen fixers increased by 29 % under CFS as a result of biological preparations use, while under no-till there was only a trend towards increasing the amount of azotobacter. The number of actinomycetes increased under the influence of CMP by 50% under direct sowing; micromycetes decreased under both farming systems. The number of cellulose-degrading microorganisms increased by 18 and 27 % under no- till and CFS, respectively. The yield of oilseed flax under no-till was 0.11 t/ha (12.9 %) higher than under conventional farming system. On average, over three years (2017-2019), an increase in yield amounted to 0.12 t/ha (19%) due to the use of microbial preparations.

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Reports on the topic "Azotobacter. Nitrogen"

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Kennedy, Christina K. Final Report: The Rhizosphere Association of the Nitrogen Fixing Bacterial Species Azotobacter Paspali with the Tropical Grass Paspalum Notatum: Specificity of Colonization and Contribution to Plant Nutrition, July 1, 1995 - February 14, 1997. Office of Scientific and Technical Information (OSTI), February 1997. http://dx.doi.org/10.2172/765727.

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