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Dissertations / Theses on the topic 'Azotobacter. Nitrogen'

Author: Grafiati

Published: 4 June 2021

Last updated: 14 February 2022

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1

Sarıbay, Gül Fidan. "Growth and nitrogen fixation dynamic of azotobacter chroococcum in nitrogen-free and omw containing medium." Ankara : METU, 2003. http://etd.lib.metu.edu.tr/upload/1098961/index.pdf.

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2

Kahindi, James H. P. "Efficiency of nitrogen fixation in Azotobacter chroococcum." Thesis, University of Sussex, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358929.

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3

Wilson, Mark Steven Michael. "Mutagenesis of nifE and nifN from Azotobacter vinelandii." Thesis, Virginia Tech, 1988. http://hdl.handle.net/10919/43071.

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The products of nifE and nifN from Azotobacter vinelandii, which are involved in the biosynthesis of the iron-molybdenum cofactor (FeMo-co) co) from nitrogenase, have been analyzed using a variety of mutagenic techniques. NifE was the object of several site-specific, amino acid substitutions that were designed to elicit information regarding metal cluster ligands, subunit-subunit interactions, and the proposed transfer of FeMo-co.from a nifEN-products complex to the apo-MoFe protein. A model of metal cluster binding; regions within the nifEN-products is discussed insofar as it relates t

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4

Brewin, Brett. "Mechanism of ammonium excretion in NifL mutants of Azotobacter vinelandii." Thesis, University of East Anglia, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323244.

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5

Saribay, Gul Fidan. "Growth And Nitrogen Fixation Dynamics Of Azotobacter Chroococcum In Nitrogen-free And Omw Containing Medium." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/1098961/index.pdf.

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Olive Mill Wastewater (OMW), by-product of oil industry, is a dark liquid with a characteristic fetid smell, bitter taste and bright appearance<br>having a high pollution potential, creating serious problems in countries producing olive oil. Azotobacter chroococcum as a Nitrogen-fixing bacteria can bioremediate OMW, by degrading its toxic constituents. With the help of this detoxification process OMW can be used as biofertilizer. In this study, the dynamics of growth and nitrogen fixation at different physiological conditions and nutrient requirements of A. chroococcum in chemically defined N-

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6

Kim, ChulHwan. "Azotobacter vinelandii nitrogenase : role of the MoFe protein [alpha]-subunit histidine-195 residue in catalysis /." This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-06062008-164937/.

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7

Burger, Eva-Maria Michaela [Verfasser], and Oliver [Akademischer Betreuer] Einsle. "Structural characterisation of nitrogen fixation by the enzyme nitrogenase of Azotobacter vinelandii." Freiburg : Universität, 2015. http://d-nb.info/1119805805/34.

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8

Setterquist, Robert Alan. "Site-directed mutagenesis of the nitrogenase MoFe protein from Azotobacter vinelandii." Thesis, Virginia Polytechnic Institute and State University, 1989. http://hdl.handle.net/10919/50091.

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A model describing the potential amino acid ligands to the four 4Fe-4S centers (P-clusters) within the Azotobacter vinelandii nitrogenase MoFe protein is presented. Based on interspecies and intersubunit amino acid comparisons of the α- and ß-subunits of the MoFe protein, and the FeMoco biosynthetic proteins, NifE and NifN, four conserved residues (Cys62, His83, Cys88, Cys154 all proposed P-cluster ligands) within the α- subunit were targeted for site-directed mutagencsis studies. In order to define a range of acceptable substitutions, 35 specific site-mutants have been constructed, each with

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9

Jacob, Jansen Philip. "Specificity and regulatory properties of the transcriptional activators VnfA and AnfA of Azotobacter vinelandii." Thesis, University of Sussex, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259964.

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10

Sippel, Daniel [Verfasser], and Oliver [Akademischer Betreuer] Einsle. "Structure of the Vanadium Nitrogenase of Azotobacter vinelandii and mechanistic insights into biological nitrogen fixation." Freiburg : Universität, 2017. http://d-nb.info/1166559327/34.

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11

Kim, ChulHwan. "Azotobacter vinelandii nitrogenase: role of the MoFe protein α-subunit histidine-195 residue in catalysis". Diss., Virginia Tech, 1994. http://hdl.handle.net/10919/38311.

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Site-directed mutagenesis and gene replacement procedures were used to isolate mutant strains of <i>Azotobacter vinelandii</i> that produce altered MoFe proteins where the α-subunit residue-195 position, normally occupied by a histidine residue, was individually substituted by a variety of other amino acids. Structural studies have revealed that this histidine residue is associated with the FeMo-cofactor binding domain and probably provides an NH→S hydrogen bond to a central bridging sulfide located within FeMo-cofactor. The present study investigates the role of the α-histidine-195 residue in

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12

Li, Hong. "Azotobacter vinelandii Nitrogenase: Multiple Substrate-Reduction Sites and Effects of pH on Substrate Reduction and CO Inhibition." Diss., Virginia Tech, 2002. http://hdl.handle.net/10919/27608.

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Mo-nitrogenase consists of two component proteins, the Fe protein and the MoFe protein. The site of substrate binding and reduction within the Mo-nitrogenase is provided by a metallocluster, the FeMo cofactor, located in the a-subunit of the MoFe protein. The FeMo cofactorâ s polypeptide environment appears to be intimately involved in the delicate control of the MoFe proteinâ s interactions with its substrates and inhibitors (Fisher K et al., 2000c). In this work, the a-subunit 278-serine residue of the MoFe protein was targeted because (i) a serine residue at this position is conserved bot

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13

Shen, Joan. "Roles of MoFe protein α-274-histidine, α-276-tyrosine and α-277-arginine residues in Azotobacter vinelandii nitrogenase catalysis". Diss., Virginia Tech, 1994. http://hdl.handle.net/10919/38211.

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14

Harris, Kelvin. "Understanding the NifM dependence of NifH in Azotobacter vinelandii functional substitution of NifH by a NifH-ChlL chimeric construct in a NifM- strain /." Master's thesis, Mississippi State : Mississippi State University, 2007. http://library.msstate.edu/etd/show.asp?etd=etd-07062007-110702.

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15

Pabon, Sanclemente Miguel Alejandro. "A Comparative Study of the Structural Features and Kinetic Properties of the MoFe and VFe Proteins from Azotobacter Vinelandii." DigitalCommons@USU, 2009. https://digitalcommons.usu.edu/etd/233.

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Biological nitrogen fixation is accomplished in the bacterium Azotobacter vinelandii by means of three metalloenzymes: The molybdenum, vanadium, and iron-only nitrogenase. The knowledge regarding biological nitrogen fixation has come from studies on the Mo-dependent reaction. However, the V- and Fe-only-dependent reduction of nitrogen remains largely unknown. By using homology modeling techniques, the protein folds that contain the metal cluster active sites for the V- and Fe-only nitrogenases were constructed. The models uncovered similarities and differences existing among the nitrogenases r

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16

Brigle, Kevin Eugene. "Studies on the structure and function of various nif and nif- associated gene products encoded within the Azotobacter vinelandii nif gene cluster." Diss., Virginia Polytechnic Institute and State University, 1989. http://hdl.handle.net/10919/54498.

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The present study investigates the structural and functional roles of the metalloclusters present within the MoFe protein of nitrogenase from Azotobacter vinelandii. A gene replacement strategy was developed for oligonucleotide-directed mutagenesis of these proteins and the resulting biological and biochemical effects of these changes were examined. Identification of structurally important regions in the MoFe protein subunits and assignment of specific amino acid residues as potential metal cluster ligands were based upon several criteria: i. metallocluster extrusion requirements; spectroscopi

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17

Edwards, Sian Eleri. "Haemoprotein synthesis in oxidase mutants of the obligate aerobe Azotobacter vinelandii and the role of cytochrome-bd during nitrogen fixation." Thesis, University of London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313764.

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18

Duda, Royce D. "Genetic Manipulation and Culturing of Azotobacter vinelandii for the Production of Nitrogenase for Use in Protein-Engineered Electrochemical Systems." Case Western Reserve University School of Graduate Studies / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1530282333596752.

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19

Shen, Joan. "Roles of MoFe protein [alpha]-274-histidine, [alpha]-276-tyrosine and [alpha]-277-arginine residues in Azotobacter vinelandii nitrogenase catalysis /." This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-06062008-162850/.

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20

Lespinat, Paul Antoine. "Métabolisme bactérien de l'hydrogène : aspects physiologiques et enzymologiques." Grenoble 1, 1988. http://www.theses.fr/1988GRE10061.

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21

Chen, Syuan-Lu, and 陳宣如. "Effects of elevated CO₂, nitrogen fertilization, and inoculated Azotobacter spp. on rice growth." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/13270573847616568615.

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碩士<br>國立中興大學<br>土壤環境科學系所<br>105<br>Rice is one of the most important food crops in the world, but climate change may affect its yield. The purpose of this study was to study the effects of CO₂ concentrations, soil types, Azotobacter inoculations, and nitrogen fertilization rates on rice growth. A factorial design experiment was carried out including two CO₂ concentrations (500 and 1000 ppm), two soil types (Dali and Houlong soils), four N application rates (0, 60, 120, and 180 kg ha-¹), and four Azotobacter inoculation treatments with a non-inoculated control. Each treatment included three rep

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