To see the other types of publications on this topic, follow the link: B-lap.
Journal articles on the topic 'B-lap'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'B-lap.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Rambaldi, A., M. Terao, S. Bettoni, ML Tini, R. Bassan, T. Barbui, and E. Garattini. "Expression of leukocyte alkaline phosphatase gene in normal and leukemic cells: regulation of the transcript by granulocyte colony- stimulating factor." Blood 76, no. 12 (December 15, 1990): 2565–71. http://dx.doi.org/10.1182/blood.v76.12.2565.2565.
Full textAbstract:
Abstract The levels of leukocyte alkaline phosphatase (LAP) messenger RNA (mRNA) are evaluated in B and T lymphocytes, monocytes, and polymorphonuclear cells (PMNs), and this transcript is found to be present only in PMNs. Precursors of the myelomonocytic pathway, represented by leukemic cells isolated from several cases of chronic myelogenous leukemia (CML) in its stable and blastic phase and acute myelogenous leukemia (AML), are devoid of LAP transcript. These data support the notion that LAP is a marker of the granulocyte terminal differentiation. Despite the absence of LAP mRNA in both the myeloid and the lymphoid precursors, nuclear run-on experiments show constitutive transcription of the LAP gene in leukemic cells obtained from AML, CML, as well as acute lymphoblastic leukemia (ALL) and B-cell chronic lymphocytic leukemia (B-CLL). In CML and in chronic myelo-monocytic leukemia (CMML) PMNs, granulocyte colony- stimulating factor (G-CSF) specifically accumulates LAP mRNA without showing a substantial increase in the rate of transcription of the LAP gene. Once increased by G-CSF, LAP mRNA is very stable, showing a half- life of more than 4 hours in the presence of actinomycin-D. G-CSF is suggested to play a pivotal role in the modulation of LAP transcript in PMNs.
2
Rambaldi, A., M. Terao, S. Bettoni, ML Tini, R. Bassan, T. Barbui, and E. Garattini. "Expression of leukocyte alkaline phosphatase gene in normal and leukemic cells: regulation of the transcript by granulocyte colony- stimulating factor." Blood 76, no. 12 (December 15, 1990): 2565–71. http://dx.doi.org/10.1182/blood.v76.12.2565.bloodjournal76122565.
Full textAbstract:
The levels of leukocyte alkaline phosphatase (LAP) messenger RNA (mRNA) are evaluated in B and T lymphocytes, monocytes, and polymorphonuclear cells (PMNs), and this transcript is found to be present only in PMNs. Precursors of the myelomonocytic pathway, represented by leukemic cells isolated from several cases of chronic myelogenous leukemia (CML) in its stable and blastic phase and acute myelogenous leukemia (AML), are devoid of LAP transcript. These data support the notion that LAP is a marker of the granulocyte terminal differentiation. Despite the absence of LAP mRNA in both the myeloid and the lymphoid precursors, nuclear run-on experiments show constitutive transcription of the LAP gene in leukemic cells obtained from AML, CML, as well as acute lymphoblastic leukemia (ALL) and B-cell chronic lymphocytic leukemia (B-CLL). In CML and in chronic myelo-monocytic leukemia (CMML) PMNs, granulocyte colony- stimulating factor (G-CSF) specifically accumulates LAP mRNA without showing a substantial increase in the rate of transcription of the LAP gene. Once increased by G-CSF, LAP mRNA is very stable, showing a half- life of more than 4 hours in the presence of actinomycin-D. G-CSF is suggested to play a pivotal role in the modulation of LAP transcript in PMNs.
3
Nagree, Yusuf, Tor N. O. Ercleve, and Peter C. Sprivulis. "After-hours general practice clinics are unlikely to reduce low acuity patient attendances to metropolitan Perth emergency departments." Australian Health Review 28, no. 3 (2004): 285. http://dx.doi.org/10.1071/ah040285.
Full textAbstract:
Objective: To model the effectiveness of afterhours general practice (GP) in reducing metropolitan Perth emergency departments? (ED) low acuity patient (LAP) attendances and costs. Methods: We estimated LAP attendances by comparison of the product of (A) the difference between self-referred and GP-referred ED discharge rates and (B) total self-referred attendances (LAP attendances = A - B). We then compared after-hours ED LAP attendance rates and costs with inner metropolitan 'working-week' ED LAP attendance rates and costs, when GP services are maximally available. Results: Working-week LAP attendances comprised 8.2% (95% CI, 8.0%?8.4%) of inner metropolitan ED attendances. Excess weekend and evening LAP attendances were estimated to comprise 16.5% (95%CI, 15.9%?17.0%) and 4.5% (95%CI, 4.1%?4.9%) of outer and inner metropolitan ED attendances respectively and totalled less than 3.0% of ED costs. Conclusions: Low acuity patients form a relatively constant, inexpensive proportion of ED workloads. After-hours GP LAP services are unlikely to significantly reduce ED attendances or costs.
4
Goel, Sachin, Ankur Gupta, Kanwar Sen, and Pooja Swami. "Kikuchi Necrotising Lymphadenitis in a Child: A Rare Case Report." Dubai Medical Journal 3, no. 3 (August 23, 2020): 126–28. http://dx.doi.org/10.1159/000509835.
Full textAbstract:
<b><i>Introduction:</i></b> Kikuchi-Fujimoto disease (KFD) is a rare benign disease with a self-limiting course. Patients usually present with clinical features of tender cervical lymphadenopathy (LAP), fever, malaise, weight loss, and night sweats. The disease may mimic infective LAP or lymphoma on clinical and histopathological examination (HPE). <b><i>Case Report:</i></b> We report a case of 3-year-old male child who presented to us with bilateral cervical LAP not responding to conservative management. Excision biopsy was done and HPE revealed KFD. <b><i>Conclusion:</i></b> Clinicians should have high index of suspicion for diagnosis of KFD in patients with cervical LAP not responding to conservative management. Excision biopsy is important for accurate diagnosis and to avoid aggressive interventions like chemotherapy.
5
Zhou, Chengcheng, Jian Wang, Peng Zhou, and Guowei Wang. "A polymerization-induced self-assembly process for all-styrenic nano-objects using the living anionic polymerization mechanism." Polymer Chemistry 11, no. 15 (2020): 2635–39. http://dx.doi.org/10.1039/d0py00296h.
Full textAbstract:
By combination of the living anionic polymerization (LAP) mechanism with the polymerization-induced self-assembly (PISA) technique, the all-styrenic diblock copolymer poly(p-tert-butylstyrene)-b-polystyrene (PtBS-b-PS) based LAP PISA was successfully developed.
6
Dargent, Jerome. "Mark J Watson, Daniel B Jones: Lap-band Companion Handbook." Obesity Surgery 18, no. 8 (June 6, 2008): 1057. http://dx.doi.org/10.1007/s11695-008-9567-y.
Full text
7
Galgaro, Maria Leticia, and Catalina Romero Lopes. "Avaliação da variabilidade isoenzimática de cinco cultivares de amendoim." Bragantia 53, no. 2 (1994): 135–40. http://dx.doi.org/10.1590/s0006-87051994000200002.
Full textAbstract:
A variabilidade genética foi avaliada dentro e entre amostras de diferentes cultivares de amendoim, Arachis hypogaea L., conhecidos como Roxo, Tatu Branco, Tatu Vermelho, Tatuí Vermelho e Tatuí (sementes com película branca), fornecidos por fazendas situadas nas regiões dos municípios de Marília, Presidente Prudente e São Manuel. Para tal análise, foi utilizada a técnica de eletroforese horizontal em gel de poliacrilamida, para os sistemas da leucil-aminopeptidase (LAP), aspartato aminotransferase (ATT) e peroxidase (PER). No sistema da leucil-aminopeptidase, foram observadas três bandas enzimáticas, denominadas LAP-A, LAP-B e LAP-C. Os padrões de bandas obtidos para o sistema da aspartato-aminotransferase mostraram a existência de três bandas anódicas, AAT-A, AAT-B e AAT-C. No sistema da peroxidase (PER), foram observadas quinze bandas, sendo oito anódicas (PER-A a PER-H) e sete catódicas (PER-I a PER-P). Os sistemas enzimáticos da peroxidase e leucil-aminopeptidase não foram discriminativos para as amostras analisadas dos diferentes cultivares obtidos nas diversas regiões. O sistema da aspartato-aminotransferase apresentou um padrão composto pelas bandas AAT-B e AAT-C, que se mostrou característico e discriminativo para as amostras do cultivar Tatu Branco, procedente de Presidente Prudente, e do 'Tatuí Vermelho', proveniente de Presidente Prudente e São Manuel.
8
Adamska, Agnieszka, Aleksandra Maria Polak, Anna Krentowska, Agnieszka Łebkowska, Justyna Hryniewicka, Monika Leśniewska, and Irina Kowalska. "Increased serum fetuin-B concentration is associated with HOMA-β and indices of liver steatosis in women with polycystic ovary syndrome: a pilot study." Endocrine Connections 8, no. 8 (August 2019): 1159–67. http://dx.doi.org/10.1530/ec-19-0243.
Full textAbstract:
Objective PCOS women are characterized by insulin resistance and have higher tendency to the development of hepatic steatosis. Fetuin-B has been introduced as a hepatokine/adipokine, which is increased in hepatic steatosis and may be connected with glucose metabolism disturbances. The aim of the study was to evaluate the relationships between serum fetuin-B concentration and indices of insulin resistance, insulin secretion and markers of liver steatosis in PCOS women in comparison to the control group. Patients and methods The study group included 108 women – 57 women with PCOS and 51 women matched for age and BMI as a control group. Serum concentration of fetuin-B was estimated. Homeostasis model assessment of insulin resistance (HOMA-IR) and homeostasis model assessment β cell function (HOMA-β) were calculated. Fatty liver index (FLI), lipid accumulation product (LAP) and visceral adiposity index (VAI) were used as markers of liver steatosis. Results We found higher serum concentration of fetuin-B and FLI in PCOS women in comparison to the control group (all P < 0.05). We observed a positive relationship between serum fetuin-B concentration and HOMA-β (r = 0.43, P = 0.01), HOMA-IR (r = 0.31, P = 0.01), FLI (r = 0.29, P = 0.02), VAI (r = 0.29, P = 0.02) and LAP (r = 0.32, P = 0.01) in PCOS women. We also noticed a relationship between HOMA-IR and FLI (r = 0.42, P = 0.01), VAI (r = 0.38, P = 0.004) and LAP (r = 0.41, P = 0.001) in this group. Multiple regression analysis revealed that HOMA-β (β = 0.39, P = 0.002) and LAP (β = 0.27, P = 0.02) were independently connected with serum fetuin-B levels in women with PCOS. Conclusions Serum fetuin-B levels are higher in women with PCOS and are independently connected with HOMA-β and hepatic steatosis.
9
Dumpies, C., T. Conrad, I. Marintschev, and G. Hofmann. "Locking Attachment Plate – Erste Erfahrungen." Zeitschrift für Orthopädie und Unfallchirurgie 150, no. 03 (June 2012): 302–8. http://dx.doi.org/10.1055/s-0031-1298530.
Full textAbstract:
Hintergrund: In dieser Arbeit stellen wir unsere ersten Erfahrungen in der Anwendung einer neuen, winkelstabilen Plattenosteosynthese vor: Locking Attachment Plate (LAP, Fa. Synthes, Oberdorf, Schweiz). Wir prüften, ob die LAP eine sinnvolle Ergänzung in der bisherigen technischen Versorgung periprothetischer Frakturen darstellt. Die LAP wird bei periprothetischen Frakturen mit stabil einliegender Endoprothese bzw. Frakturen um intramedulläre Implantate angewandt. Die Plattenosteosynthese wird z. B. auf eine 4,5-mm Locking Compression Plate (LCP) installiert. Über ihre 4 diagonal angebrachten Arme können Verriegelungs- oder Kortikalisschrauben winkelstabil, bikortikal um den Prothesenschaft in den Knochen eingebracht werden. Die LAP soll den lateralen Schraubenausbruch verhindern, den Prothesenschaft stabilisieren und somit die frühzeitige postoperative Mobilisierung der Patienten ermöglichen. Patienten und Methoden: Wir implantierten die LAP mittels operativer Frakturbehandlung in ORIF-Technik bei 17 Patienten mit periprothetischen Frakturen an Femur, Tibia und Humerus. Der Nachbeobachtungszeitraum betrug mindestens 13 Monate. Ergebnisse: Es entgingen 2 Patienten der Verlaufsbeobachtung, da sie wenige Wochen postoperativ aufgrund ihrer Begleitmorbiditäten starben. Insgesamt kam es in 2 von 17 Fällen zum Infektgeschehen (11,8 %). Es wurden 15 Patienten nachuntersucht. Davon erlangten 11 Patienten ihren ursprünglichen Bewegungsumfang (ROM) und 9 Patienten ihre Ausgangsmobilität wieder. Alle Patienten mit periprothetischen Humerusfrakturen verzeichneten muskuläre Defizite. In 5 von 15 Fällen (33,3 %) konnte zumindest radiologisch keine Frakturheilung festgestellt werden. Dennoch lag bei 14 von 15 Patienten das Implantat stabil ein, ohne Zeichen einer Implantat- oder Prothesenlockerung. Es trat kein Fall von Materialversagen auf. Schlussfolgerung: Periprothetische Frakturen sind eine immer häufiger werdende Komplikation beim alten, oft multimorbiden Patienten. Eine rasche therapeutische Intervention und die frühzeitige postoperative Mobilisierung tragen entscheidend zum Behandlungserfolg bei. Die ersten Ergebnisse in der Anwendung der LAP als neues Implantat zur Versorgung periprothetischer Frakturen an Femur, Tibia und Humerus sind vielversprechend. Es bedarf weiterer Studien, um zu zeigen, ob unsere insgesamt guten Ergebnisse in größeren Patientenkollektiven reproduzierbar sind und die LAP einen festen Stellenwert im technischen Repertoire zur Therapie periprothetischer Frakturen einnehmen kann.
10
Radojicic, Ratko, Snezana Spasic, Zorica Saicic, Tamara Jovanovic, and Jovana Simic-Krstic. "Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells." Journal of the Serbian Chemical Society 69, no. 12 (2004): 1005–11. http://dx.doi.org/10.2298/jsc0412005r.
Full textAbstract:
The C3 clone of B-16 mouse melanoma was cultured for 1, 6 and 9 days and analyzed. The changes which are not directly linked to melanogenesis in the B-16 / C3 cultures during their maturation were characterized. Early (1 day) confluent (6 days) and old (9 days) cell cultures are distinguished by their leucine aminopeptidase (LAP) and ?-naphthyl acetate esterase (ANAE) isoenzyme patterns. Both quantitative and qualitative changes in LAP and ANAE isoenzyme can be observed during culture maturation. There is an increase in the activity of the enzyme copper, zinc-containing superoxide-dismutase (CuZn SOD). The increaase in the CuZn SOD enzyme activity might be related to B-16/C3 cell melanogenesis and / or to differentiation.
11
Rademaker, Miriam T., Christopher J. Charles, Iain C. Melton, A. Mark Richards, Christopher M. Frampton, Jeff Siou, Fujian Qu, Neal L. Eigler, Dan Gutfinger, and Richard W. Troughton. "Monitoring of heart failure: comparison of left atrial pressure with intrathoracic impedance and natriuretic peptide measurements in an experimental model of ovine heart failure." Clinical Science 120, no. 5 (November 19, 2010): 207–17. http://dx.doi.org/10.1042/cs20100388.
Full textAbstract:
Monitoring of HF (heart failure) with intracardiac pressure, intrathoracic impedance and/or natriuretic peptide levels has been advocated. We aimed to investigate possible differences in the response patterns of each of these monitoring modalities during HF decompensation that may have an impact on the potential for early therapeutic intervention. Six sheep were implanted with a LAP (left atrial pressure) sensor and a CRT-D (cardiac resynchronization therapy defibrillator) capable of monitoring impedance along six lead configuration vectors. An estimate of ALAP (LAP from admittance) was determined by linear regression. HF was induced by rapid ventricular pacing at 180 and 220 bpm (beats/min) for a week each, followed by a third week with daily pacing suspensions for increasing durations (1–5 h). Incremental pacing induced progressively severe HF reflected in increases in LAP (5.9 ± 0.4 to 24.5 ± 1.6 mmHg) and plasma atrial (20 ± 3 to 197 ± 36 pmol/l) and B-type natriuretic peptide (3.7 ± 0.7 to 32.7 ± 5.4 pmol/l) (all P<0.001) levels. All impedance vectors decreased in proportion to HF severity (all P<0.001), with the LVring (left ventricular)-case vector correlating best with LAP (r2=0.63, P<0.001). Natriuretic peptides closely paralleled rapid acute changes in LAP during alterations in pacing (P<0.001), whereas impedance changes were delayed relative to LAP. ALAP exhibited good agreement with LAP. In summary, impedance measured with an LV lead correlates significantly with changes in LAP, but exhibits a delayed response to acute alterations. Natriuretic peptides respond rapidly to acute LAP changes. Direct LAP, impedance and natriuretic peptide measurements all show promise as early indicators of worsening HF. ALAP provides an estimate of LAP that may be clinically useful.
12
Liu, Fangcen, Jielei He, Ye Zhu, Hongdong Wang, Wenhuan Feng, Xitai Sun, Yan Bi, and Dalong Zhu. "Body Adiposity Index Is Predictive of Weight Loss after Roux-en-Y Gastric Bypass." Annals of Nutrition and Metabolism 77, no. 3 (2021): 168–77. http://dx.doi.org/10.1159/000516522.
Full textAbstract:
<b><i>Background/Aims:</i></b> Roux-en-Y gastric bypass (RYGB) is one of the most effective therapies for morbid obesity, yet some patients who have taken the surgery still undergo insufficient weight loss. Visceral adiposity index (VAI), lipid accumulation product (LAP), body adiposity index (BAI), and cardiometabolic index (CMI) have been regarded as clinical indicators of adiposity phenotypes that associated closely with obesity-related metabolic diseases. However, no studies have evaluated the relationship between these indexes and weight loss after bariatric surgery. In this prospective study, we aimed to evaluate whether VAI, LAP, BAI, and CMI would predict postoperative weight loss outcomes after RYGB. <b><i>Methods:</i></b> This study included 38 men and 67 women who have undergone RYGB between January 2017 and May 2018 and recorded their %TWL (percent of total weight loss), %EBMIL (percent of excess body mass index loss), %EWL (percent of excess weight loss), anthropometric indices, and biochemical parameters before and 12 months after the surgery. In addition, VAI, LAP, BAI, and CMI were measured with anthropometric measures or lipid profiles using related equations and analyzed with metabolic characteristics. <b><i>Results:</i></b> Subjects with lower BAI (<32.54 in men and 37.39 in women) displayed higher %EBMIL and %EWL 12 months after surgery. BAI was independently associated with %EWL 12 months after surgery in both men and women (both <i>p</i> < 0.05). The area under the receiver operating characteristic curve for BAI was significantly higher (0.773 in men and 0.818 in women) than VAI, LAP, and CMI. <b><i>Conclusions:</i></b> BAI serves as a reliable surrogate marker of the weight loss outcome after RYGB. The predictivity of adiposity indexes in beneficial outcomes after weight loss therapies is of important referential value for the implementation and optimization of individualized and refined weight loss treatments for obese patients.
13
He, Changshu, Zhiqiang Zhang, Ying Li, Jingxun Wei, Menggang Zhai, Su Zhao, and Xiang Zhao. "Interface Characteristics and Mechanical Properties of Ultrasonic-Assisted Friction Stir Lap Welded 7075-T6 Aluminium Alloy." Materials 13, no. 23 (November 25, 2020): 5335. http://dx.doi.org/10.3390/ma13235335.
Full textAbstract:
In this work, friction stir lap welding (FSLW) and ultrasonic-assisted friction stir lap welding (UAFSLW) was applied to 6-mm-thick 7075-T6 alloy sheets using three welding tools with the same process parameters. The joint formation, microstructural characteristics, and mechanical properties of the resulting lap joints were then investigated. The results showed that ultrasonic vibration significantly promoted the flow of metal at the interface, enlarged the size of the stirred zone (SZ), and reduced the angle between the hook defect and the interface. During lap shear testing, the FSLW and UAFSLW joints fractured in a similar manner. The fracture modes included tensile fracture, shear fracture, and a mixture of both. Cold lap and hook defects may have served as crack-initiation zones within the joint. Under configuration A (i.e., upper sheet on the retreating side (RS)), all joints failed in the shear-fracture mode. The effective lap width (ELW) of the joint welded using tool T2 was the greatest. This resulted in a higher shear fracture strength. The maximum shear fracture strength of the UAFSLW joint was 663.1 N/mm. Under configuration B (i.e., upper sheet on the advancing side (AS)), the shear fracture strength was greatly affected by the fracture mode. The highest shear fracture strength of the UAFSLW joint, 543.7 N/mm, was welded by tool T3. Thus, under otherwise identical conditions, UAFSLW joints can withstand a greater fracture shear strength than FSLW joints, as ultrasonic vibration helps to mix the material at the interface, thus, enlarging the SZ and diminishing the cold lap defects.
14
Sharma, Eeshita, Manjit Kumar, Rohit Sharma, Ajay Bansal, and Sumit Katoch. "Comparative Evaluation of Bond Strength between Ridge Lap Surface of Acrylic Teeth and Denture Base Resin: An In Vitro Study." Dental Journal of Advance Studies 07, no. 01 (March 28, 2019): 012–18. http://dx.doi.org/10.1055/s-0039-1684881.
Full textAbstract:
Abstract Statement of Problem Acrylic teeth are preferred to porcelain teeth in dentures as they unite chemically with denture base resin, but their fracture from denture is common. Purpose The purpose of this study was to improve the bond strength of denture teeth to acrylic resin denture base by chemical or mechanical modification of the ridge lap surface of denture teeth. Materials and Methods Total 100 artificial cross-linked acrylic resin central incisors were divided into five groups: group A, 20 samples without modification (control group); group B, 20 samples (ridge lap surface of teeth treated with monomer); group C, 20 samples (ridge lap surface of teeth treated with monomer and the glaze layer removed with aluminum oxide abrasive stone); group D, 20 samples (ridge lap surface of teeth treated with dichloromethane); and group E, 20 samples (dichloromethane application followed by abrasion with aluminum oxide stone on ridge lap surface of teeth). They were mounted on wax blocks, and the blocks were acrylized. The bond strength values were obtained by subjecting the samples to shear compressive load under universal testing machine. Result The results were subjected to statistical analysis by applying analysis of variance and Bonferroni test for multiple group comparisons, and graphs were plotted. The mean value of bond strength was highest for group E (modified by aluminum oxide abrasion prior to dichloromethane application), followed by group C (modified by aluminum oxide abrasion prior to monomer application), group D (modified by dichloromethane application), group B (modified by monomer application), and lastly group A (control group). Significantly improved bond strength values were obtained in modified groups as compared with the control group. Conclusion Dichloromethane application followed by aluminum oxide abrasion provided the highest bond strength and is recommended to prevent debonding of the teeth from the denture base.
15
Wong, Stuart J., Pedro A. Torres-Saavedra, Nabil F. Saba, George Shenouda, Jeffrey Bumpous, Robert E. Wallace, Christine H. Chung, et al. "TRYHARD, a randomized phase II trial (RTOG Foundation 3501) of concurrent accelerated radiation plus cisplatin (cis) with or without lapatinib (Lap) for stage III- IV Non-HPV head and neck carcinoma (HNC)." Journal of Clinical Oncology 39, no. 15_suppl (May 20, 2021): 6014. http://dx.doi.org/10.1200/jco.2021.39.15_suppl.6014.
Full textAbstract:
6014 Background: Chemoradiation (CRT) with cis or anti-EGFR Ab has been shown to improve survival of patients with stage III-IV HNC. Since Lap, a dual EGFR and HER2 inhibitor, has shown effectiveness with CRT in a pilot non-HPV HNC cohort, the RTOG Foundation launched a phase II trial to test the hypothesis that adding Lap to the RT-cis for frontline therapy of stage III-IV Non-HPV HNC improves progression-free survival (PFS). Methods: Patients with stage III-IV carcinoma of the oropharynx (p16-negative), larynx, and hypopharynx, having Zubrod performance of 0-1, and meeting predefined blood chemistry criteria were enrolled after providing consent. Patients were randomized (1:1) to 70 Gy (6 weeks) + 2 cycles of CDDP (q3 weeks) plus either Lap (1500 mg daily, Arm A) or placebo (Arm B) starting 1 week prior to RT and concurrent with RT and for 3 months post RT. PFS was the primary endpoint. The protocol specified 69 PFS events (142 patients) for the final analysis based on HR = 0.65, 80% power, 1-sided alpha 0.20, and one interim efficacy and futility analysis at 50% information. PFS rates between arms for all randomized patients were compared by 1-sided log-rank test (1-sided alpha 0.1803). Overall survival (OS) was a secondary endpoint. Results: From 10/’12 to 04/’17, 142 patients were enrolled, of whom 127 were randomized, 63 to Arm A and 64 to Arm B. Arms A vs B, respectively, were similar in baseline patient characteristics, radiation delivery, completing ≥ 70 Gy (85.7% vs. 82.8%) and cisplatin delivery, completing 200 (±5%) mg/m2 (65.1% vs 70.3%), but dissimilar in Lap/placebo delivery (median dose, 87000 mg vs. 125250 mg). Median follow-up was 4.1 years for surviving patients. The final analysis suggests no improvement in PFS of adding Lap to CRT (HR [A/B]: 0.91, 95% confidence interval CI 0.56-1.46; P= 0.34; 2-year rates: 50.6%, CI 37.5-63.7% vs. 56.2% CI 43.0-69.4%), or in OS (HR: 1.06, CI 0.61-1.86; P = 0.58; 2-year rates: 71.8% CI 60.1-83.5% vs. 76% CI 64.5-87.4%), death within 30 days of therapy (3.3% vs. 3.4%), and overall treatment-related grade 3-5 adverse event rate (86.7% vs. 84.7%). Grade 3-4 mucositis rates on Arm A and Arm B were 21.7% vs. 23.7%, all grade dysphagia and rash rates were 43.3% vs. 59.3%, and 13.3% vs. 6.8%, respectively. Conclusions: The addition of Lap to the radiation-cisplatin platform did not improve progression-free or overall survival in unselected non-HPV HN. Thus, dual EGFR, HER-2 inhibition does not appear to enhance the effects of chemoradiation. Although we showed that accrual to a non-HPV HN specific trial is feasible, new strategies must be investigated to improve the outcome for this poor prognosis HN population.
16
Rao, N. Venugopal, D. Lakshmayya Naidu, JSV Bharat, M. Venugopal Reddy, and P. Naveen. "Effect of Trichloromethane on the Bond Strengths between Acrylic Teeth and Different Heat-cured Denture Bases: A Comparative Study." Journal of Contemporary Dental Practice 13, no. 6 (2012): 850–55. http://dx.doi.org/10.5005/jp-journals-10024-1241.
Full textAbstract:
ABSTRACT Aim This study is to evaluate the role of 1:1 v/v 30% trichloromethane and monomer solvent in enhancing the durability of bonding between cross-linked acrylic teeth and different heatcured denture bases with or without mechanical preparations made on ridge lap portion of the artificial teeth. Materials and methods Two high impact denture base resin materials (Trevalon HI, DeTrey, UK, and DPI Tuff, Mumbai) and one nonhigh impact denture base resin material (DPI Quick Set, Mumbai) were selected to form three groups. Each group contains 30 specimens prepared by five different methods. A mixture of 30% trichloromethane and monomer, mixed in the ratio of 1:1 and applied for 1 minute on the ridge lap area of experimental specimens of methods—B, C, D and E (Specimens of method—A being control group, where no alterations were made at the ridge lap portion of acrylic teeth) before curing. Hounsfield universal testing machine is employed to evaluate the comparative bond strengths. Results No significant difference was seen in bond strengths between specimens of experimental methods in all groups. When each group was assessed separately method B specimens in group 1 (739.2 N), group 2 (758 N) and method D specimens in group 3 (729 N) showed highest mean bond strengths. Control group specimens showed the least bond strength (400-460 N) in all groups with more adhesive failures. Conclusion Ridge lap portion of the specimens treated with chemical solvent as in method B showed increased bond strength in groups 1 and 2. Hence, this is a preferred method. Clinical significance Evaluation of effect of different chemical and mechanical preparations at the ridge lap areas of acrylic teeth before acrylization helps the clinician and technician to overcome the problem of debonding of teeth from denture bases and in turn provides better quality prosthesis to the patient. How to cite this article Bharat JSV, Naidu DL, Reddy MV, Naveen P, Rao NV. Effect of Trichloromethane on the Bond Strengths between Acrylic Teeth and Different Heat-cured Denture Bases: A Comparative Study. J Contemp Dent Pract 2012;13(6):850-855.
17
Branco-de-Almeida, L. S., Y. Cruz-Almeida, Y. Gonzalez-Marrero, H. Huang, I. Aukhil, P. Harrison, S. M. Wallet, and L. M. Shaddox. "Local and Plasma Biomarker Profiles in Localized Aggressive Periodontitis." JDR Clinical & Translational Research 2, no. 3 (April 14, 2017): 258–68. http://dx.doi.org/10.1177/2380084417701898.
Full textAbstract:
Localized aggressive periodontitis (LAP) patients possess a systemic hyperinflammatory response after lipopolysaccharide stimulation. However, the levels of inflammatory and bone biomarkers in plasma, as well as possible associations between local and plasma biomarkers, are unknown in LAP. This cross-sectional study aimed to characterize gingival crevicular fluid (GCF) and plasma biomarker profiles in LAP patients, their healthy siblings (HS), and healthy unrelated controls (HC). Fifty-eight LAP subjects, 33 HS, and 49 HC (African Americans, aged 5 to 25 y) were included. Following collection of clinical parameters with GCF and plasma samples, levels of 16 inflammatory and bone resorption biomarkers were determined with Milliplex. Univariate and correlation analyses were performed among all clinical and laboratorial parameters. Discriminant analyses were used to investigate groups of biomarkers discriminating LAP from HS and HC in GCF and plasma. GCF levels of multiple cytokines and chemokines and RANKL:OPG ratio (receptor activator of nuclear factor kappa-B ligand:osteoprotegerin) were higher in LAP disease, most of which positively correlated with probing depth and attachment loss of sampled sites. A group of IL-12p40, IL-6, IL-12p70, IL-2, and MIP-1α discriminated LAP diseased sites from twheir healthy sites, as well as from HS and HC healthy sites. In plasma, only RANKL levels were increased in LAP versus controls, which positively correlated with the percentage of affected sites and deep/bleeding sites. A plasma inflammatory profile including MIP-1α, IL-8, IL-10, and INF-γ could significantly discriminate LAP patients from HS and HC. No correlations were found between GCF and plasma levels of biomarkers. In conclusion, an inflammatory profile including groups of specific biomarkers in GCF and plasma may significantly discriminate LAP from healthy individuals. The hyperinflammatory response previously found in the peripheral blood of LAP patients is dependent on lipopolysaccharide stimulation, apparently resulting mostly in local tissue destruction and changes in biomarker profile, with a slight influence in the systemic inflammatory profile ( ClinicalTrials.gov NCT01330719). Knowledge Transfer Statement: The results of this study can be possibly used by clinicians in the future as diagnostic tools for localized aggressive periodontitis. Thus, in the future, with proper consideration of cost, patient preference, chair-side feasibility and ultimately further studies validating the role of GCF markers for disease progression and response to treatment, this information could lead to more appropriate therapeutic decisions and the development of preventive approaches for susceptible patients.
18
Konings, Marco J., Olaf S. Noorbergen, David Parry, and Florentina J. Hettinga. "Pacing Behavior and Tactical Positioning in 1500-m Short-Track Speed Skating." International Journal of Sports Physiology and Performance 11, no. 1 (January 2016): 122–29. http://dx.doi.org/10.1123/ijspp.2015-0137.
Full textAbstract:
Purpose:To gain more insight in pacing behavior and tactical positioning in 1500-m short-track speed skating, a sport in which several athletes directly compete in the same race.Methods:Lap times and intermediate rankings of elite 1500-m short-track-skating competitors were collected over the season 2012–13 (N = 510, 85 races). Two statistical approaches were used to assess pacing behavior and tactical positioning. First, lap times were analyzed using a MANOVA, and for each lap differences between sex, race type, final rankings, and stage of competition were determined. Second, Kendall tau b correlations were used to assess relationships between intermediate and final rankings. In addition, intermediate rankings of the winner of each race were examined.Results:In 1500 m (13.5 laps of 111.12 m), correlations between intermediate and final ranking gradually increased throughout the race (eg, lap 1, r = .05; lap 7, r = .26; lap 13, r = .85). Moreover, the percentage of race winners skating in the leading position was over 50% during the last 3 laps. Top finishers were faster than bottom-place finishers only during the last 5 laps, with on average 0.1- to 1.5-s faster lap times of the race winners compared with the others during the last 5 laps.Conclusions:Although a fast start led to faster finishing times, top finishers were faster than bottom-placed finishers only during the last 5 laps. Moreover, tactical positioning at 1 of the foremost positions during the latter phase of the race appeared to be a strong determinant of finishing position.
19
Noorbergen, Olaf S., Marco J. Konings, Dominic Micklewright, Marije T. Elferink-Gemser, and Florentina J. Hettinga. "Pacing Behavior and Tactical Positioning in 500- and 1000-m Short-Track Speed Skating." International Journal of Sports Physiology and Performance 11, no. 6 (September 2016): 742–48. http://dx.doi.org/10.1123/ijspp.2015-0384.
Full textAbstract:
Purpose:To explore pacing behavior and tactical positioning during the shorter 500- and 1000-m short-track competitions.Methods:Lap times and intermediate rankings of elite 500- and 1000-m short-track-skating competitors were collected over the 2012–13 season. First, lap times were analyzed using a MANOVA, and for each lap, differences between sex, race type, final ranking, and stage of competition were determined. Second, Kendall tau-b correlations were used to assess relationships between intermediate and final rankings. In addition, intermediate rankings of the winner of each race were examined.Results:Top-placed athletes appeared faster than bottom-placed athletes in every lap in the 500-m, while in the 1000-m no differences were found until the final 4 laps (P < .05). Correlations between intermediate and final rankings were already high at the beginning stages of the 50-m (lap 1: r = .59) but not for the 1000-m (lap 1: r = .21).Conclusions:Although 500- and 1000-m short-track races are both relatively short, fundamental differences in pacing behavior and tactical positioning were found. A fast-start strategy seems to be optimal for 500-m races, while the crucial segment in 1000-m races seems to be from the 6th lap to the finish line (ie, after ± 650 m). These findings provide evidence to suggest that athletes balance between choosing an energetically optimal profile and the tactical and positional benefits that play a role when riding against an opponent, as well as contributing to developing novel insights in exploring athletic behavior when racing against opponents.
20
Gianni, M., M. Terao, P. Norio, T. Barbui, A. Rambaldi, and E. Garattini. "All-trans retinoic acid and cyclic adenosine monophosphate cooperate in the expression of leukocyte alkaline phosphatase in acute promyelocytic leukemia cells." Blood 85, no. 12 (June 15, 1995): 3619–35. http://dx.doi.org/10.1182/blood.v85.12.3619.bloodjournal85123619.
Full textAbstract:
Treatment of acute promyelocytic leukemia (APL) blasts with cyclic adenosine monophosphate (cAMP) analogs, in combination with all-trans retinoic acid (ATRA), results in the upregulation of the expression of leukocyte alkaline phosphatase (LAP), a marker for the differentiation of the granulocyte. The synergistic interaction between the cyclic nucleotide analogs and the retinoid is not unique to APL cells, as it is observed also in the peripheral granulocytes of chronic myelogenous leukemia (CML) patients. The molecular mechanisms underlying LAP induction were studied in NB4, an immortalized APL cell line. Induction of LAP enzymatic activity is dependent on the time of exposure and on the concentrations of dibutyryl-cAMP or 8-bromo-cAMP and ATRA, two factors that influence the kinetics of appearance of detectable levels of the enzyme. Augmentation of LAP levels by ATRA and cAMP is the result of both transcriptional and early posttranscriptional events and requires de novo protein synthesis. LAP induction correlates with augmentation in the levels of the type I catalytic subunit of cAMP-dependent protein kinase transcript and with granulocytic differentiation. The transcriptional component of the process leading to increased LAP gene expression was reproduced in its main features by transient transfection experiments performed in COS-7 cells using the normal retinoic acid receptor type alpha (RAR-alpha) or the APL-specific aberrant form (PML-RAR) and the upstream promoter of the liver/bone/kidney (L/B/K)-type alkaline phosphatase gene. The promoter is upregulated by treatment with ATRA, and this upregulation is further increased by cAMP analogs.
21
GENG, Y. L., D. XU, D. L. SUN, X. Q. WANG, G. H. ZHANG, and T. B. LI. "GROWTH HILLOCKS ON THE {100} FACES OF L-ARGININE PHOSPHATE MONOHYDRATE SINGLE CRYSTALS INVESTIGATED BY ATOMIC FORCE MICROSCOPY." Surface Review and Letters 11, no. 01 (February 2004): 71–75. http://dx.doi.org/10.1142/s0218625x04005913.
Full textAbstract:
Growth hillocks on the {100} faces of L-arginine phosphate monohydrate (LAP) single crystals grown at 25°C and at a supersaturation of 0.32 have been discussed. The typical dislocation growth hillocks are lopsided and elongate along the b direction. The dislocation sources are probably caused by the extra stress field which is introduced by the hollow cavities distributing on the steps and hillocks generated by the two-dimensional nucleus. The elongated shape is due to the characteristic structure of the LAP crystal. Apart from that, the formation of the lopsided growth hillocks is explained by the liquid flow theory.
22
Furukawa, K. "LAP2 binding protein 1 (L2BP1/BAF) is a candidate mediator of LAP2-chromatin interaction." Journal of Cell Science 112, no. 15 (August 1, 1999): 2485–92. http://dx.doi.org/10.1242/jcs.112.15.2485.
Full textAbstract:
Lamina-associated polypeptide (LAP) 2, which directly interacts with B-type lamins and chromosomes, is an integral membrane protein specifically distributed along the inner nuclear membrane of the nuclear envelope. The chromatin- and lamin-binding activity of LAP2 suggests that LAP2 plays an important role in targeting mitotic vesicles to chromosomes and reorganizing the nuclear structure at the end of mitosis. Here I identified a LAP2 interacting protein, termed L2BP1 (LAP2 binding protein 1). The rat L2BP1 cDNA sequence is predicted to encode a protein of 89 amino acids which turns out to be a rat homolog of mouse and human BAF (Barrier-to-Autointegration Factor). L2BP1 is distributed diffusely throughout the nucleus in interphase cells. It is, however, highly concentrated at the chromosomes during the M-phase. Further, the L2BP1 binding domain of LAP2 overlaps its chromosome-binding region. These findings suggest that L2BP1 is a candidate mediator of LAP2-chromosome interaction at the end of mitosis.
23
Mathew, Zacharia, Tina M. Knox, and Charles G. Miller. "Salmonella enterica Serovar Typhimurium Peptidase B Is a Leucyl Aminopeptidase with Specificity for Acidic Amino Acids." Journal of Bacteriology 182, no. 12 (June 15, 2000): 3383–93. http://dx.doi.org/10.1128/jb.182.12.3383-3393.2000.
Full textAbstract:
ABSTRACT Peptidase B (PepB) of Salmonella enterica serovar Typhimurium is one of three broad-specificity aminopeptidases found in this organism. We have sequenced the pepB gene and found that it encodes a 427-amino-acid (46.36-kDa) protein, which can be unambiguously assigned to the leucyl aminopeptidase (LAP) structural family. PepB has been overexpressed and purified. The active enzyme shows many similarities to other members of the LAP family: it is a heat-stable (70°C; 20 min) hexameric (∼270-kDa) metallopeptidase with a pH optimum of 8.5 to 9.5. A detailed study of the substrate specificity of the purified protein shows that it differs from other members of the family in its ability to hydrolyze peptides with N-terminal acidic residues. The preferred substrates for PepB are peptides with N-terminal Asp or Glu residues. Comparison of the amino acid sequence of PepB with those of other LAPs leads to the conclusion that PepB is the prototype of a new LAP subfamily with representatives in several other eubacterial species and to the prediction that the members of this family share the ability to hydrolyze peptides with N-terminal acidic residues. Site-directed mutagenesis has been used to show that this specificity appears to be determined by a single Lys residue present in a sequence motif conserved in all members of the subfamily.
24
Chen, Yi, Yingchao Chen, Ningjian Wang, Chi Chen, Xiaomin Nie, Qin Li, Bing Han, and Yingli Lu. "Thyroid Stimulating Hormone Within the Reference Range is Associated with Visceral Adiposity Index and Lipid Accumulation Product: A Population-Based Study of SPECT-China." Hormone and Metabolic Research 50, no. 01 (November 17, 2017): 29–36. http://dx.doi.org/10.1055/s-0043-122235.
Full textAbstract:
AbstractFew studies about the relationship of thyroid stimulating hormone (TSH) level with visceral adiposity index (VAI) and lipid accumulation product (LAP) in large Chinese population are available. The aim of this study was to explore whether TSH level within the reference range was associated with these two newer reliable cardiovascular risk factors. The data were obtained from a cross-sectional study (SPECT-China study, 2014–2015), which was based on the population. Participants underwent several checkups, which included anthropometric parameters, blood pressure, TSH levels, and glucose and lipid profiles. VAI and LAP were calculated. A total of 8727 subjects were enrolled in this study. The mean age was 53.00±13.05 years. Age, female to male ratio, BMI, systolic pressure, and HOMA-IR increased along with the increasing levels of TSH (p<0.01). Analysis of lipid profile showed significantly higher serum triglycerides and LDL cholesterol levels in the higher TSH quartile (p<0.001 and p=0.028, respectively). The levels of VAI and LAP were all increased along with increasing TSH level (all p<0.001). After full adjustment, TSH levels were positively associated with VAI, LAP, and TG/HDL levels significantly (B=0.041, 0.028 and 0.037, respectively, all p<0.01). Higher TSH concentrations among the reference range were common in older people as well as in women subjects, and they were positively associated with VAI and LAP. This highlighted that even subtle variations of serum TSH within the normal range may be potential risk factors for cardiovascular diseases.
25
Gianni, M., M. Terao, S. Zanotta, T. Barbui, A. Rambaldi, and E. Garattini. "Retinoic acid and granulocyte colony-stimulating factor synergistically induce leukocyte alkaline phosphatase in acute promyelocytic leukemia cells." Blood 83, no. 7 (April 1, 1994): 1909–21. http://dx.doi.org/10.1182/blood.v83.7.1909.1909.
Full textAbstract:
Abstract In this report we show a strong synergistic interaction between granulocyte colony-stimulating factor (G-CSF) and all-trans retinoic acid (ATRA) on the expression of leukocyte alkaline phosphatase (LAP) in freshly isolated acute promyelocytic leukemia (APL) blasts as well as in NB40 and HL-60 cell lines. The strong synergism observed in these cell types was not evident in two acute leukemia cell lines (K562 and GF-D8), in normal granulocytes, and in monocytes. In freshly isolated leukocytes derived from chronic myelogenous leukemia (CML), in the stable phase of the disease, a weaker interaction between ATRA and G- CSF was documented. The cross-talk between the cytokine and the retinoid was studied in detail in NB4, an immortalized APL leukemia cell line, retaining the 15′17 chromosomal translocation involving the retinoic acid receptor type alpha. The treatment of NB4 cells with G- CSF alone or ATRA alone leads to no increase and to minor induction in LAP activity, respectively. If the cells are treated with the two compounds simultaneously, a dramatic elevation of LAP is observed after 4 days. The synergism between G-CSF and ATRA is evident at concentrations of the retinoid between 10(-7) and 10(-5) mol/L and at concentrations of the cytokine between 1 and 10 ng/mL. The simultaneous presence of the two compounds is necessary to obtain maximal increase of LAP activity and the effect is cell density-dependent. Synergism is specific for G-CSF, and it is not observed with other cytokines and functional inducers of the granulocyte. The augmentation of LAP activity is the consequence of an increased transcriptional rate of the liver/bone/kidney-type (L/B/K-type) alkaline phosphatase gene, as determined by Northern blotting and nuclear run-on analysis using specific cDNA probes. Only one of the two possible alternatively spliced forms of L/B/K-type alkaline phosphatase transcript is detected in NB4 cells after stimulation with G-CSF and ATRA. This mRNA form, which is the one observed in normal polymorphonuclear leukocytes, contains the most upstream leader exon. In NB4 cells, ATRA induces G- CSF, alpha, and beta retinoic acid receptor transcripts, whereas G-CSF has minor effects on the expression of these mRNAs.
26
Gianni, M., M. Terao, S. Zanotta, T. Barbui, A. Rambaldi, and E. Garattini. "Retinoic acid and granulocyte colony-stimulating factor synergistically induce leukocyte alkaline phosphatase in acute promyelocytic leukemia cells." Blood 83, no. 7 (April 1, 1994): 1909–21. http://dx.doi.org/10.1182/blood.v83.7.1909.bloodjournal8371909.
Full textAbstract:
In this report we show a strong synergistic interaction between granulocyte colony-stimulating factor (G-CSF) and all-trans retinoic acid (ATRA) on the expression of leukocyte alkaline phosphatase (LAP) in freshly isolated acute promyelocytic leukemia (APL) blasts as well as in NB40 and HL-60 cell lines. The strong synergism observed in these cell types was not evident in two acute leukemia cell lines (K562 and GF-D8), in normal granulocytes, and in monocytes. In freshly isolated leukocytes derived from chronic myelogenous leukemia (CML), in the stable phase of the disease, a weaker interaction between ATRA and G- CSF was documented. The cross-talk between the cytokine and the retinoid was studied in detail in NB4, an immortalized APL leukemia cell line, retaining the 15′17 chromosomal translocation involving the retinoic acid receptor type alpha. The treatment of NB4 cells with G- CSF alone or ATRA alone leads to no increase and to minor induction in LAP activity, respectively. If the cells are treated with the two compounds simultaneously, a dramatic elevation of LAP is observed after 4 days. The synergism between G-CSF and ATRA is evident at concentrations of the retinoid between 10(-7) and 10(-5) mol/L and at concentrations of the cytokine between 1 and 10 ng/mL. The simultaneous presence of the two compounds is necessary to obtain maximal increase of LAP activity and the effect is cell density-dependent. Synergism is specific for G-CSF, and it is not observed with other cytokines and functional inducers of the granulocyte. The augmentation of LAP activity is the consequence of an increased transcriptional rate of the liver/bone/kidney-type (L/B/K-type) alkaline phosphatase gene, as determined by Northern blotting and nuclear run-on analysis using specific cDNA probes. Only one of the two possible alternatively spliced forms of L/B/K-type alkaline phosphatase transcript is detected in NB4 cells after stimulation with G-CSF and ATRA. This mRNA form, which is the one observed in normal polymorphonuclear leukocytes, contains the most upstream leader exon. In NB4 cells, ATRA induces G- CSF, alpha, and beta retinoic acid receptor transcripts, whereas G-CSF has minor effects on the expression of these mRNAs.
27
Zhao, Yaxian, Jincheng Du, Xin Cao, Chong Zhang, Gang Xu, Xvsheng Qiao, Yong Liu, Shou Peng, and Gaorong Han. "A modified random network model for P2O5–Na2O–Al2O3–SiO2 glass studied by molecular dynamics simulations." RSC Advances 11, no. 12 (2021): 7025–36. http://dx.doi.org/10.1039/d0ra10810c.
Full textAbstract:
(A) A modified structural model proposed for P2O5-bearing sodium aluminosilicate glasses. (B) Degree of preferred connection (DPC) of different T–O–T network linkage for LAP, MAP and HAP glass compositions with various P2O5 content.
28
Atsumi, Yosuke, Masakatsu Numata, Toru Aoyama, Tsutomu Hayashi, Naoto Yamamoto, Takashi Oshima, Norio Yukawa, et al. "Safety and feasibility of laparoscopic surgery for colorectal cancer in elderly patients (YSURG1401 study)." Journal of Clinical Oncology 34, no. 4_suppl (February 1, 2016): 725. http://dx.doi.org/10.1200/jco.2016.34.4_suppl.725.
Full textAbstract:
725 Background: The safety and feasibility of laparoscopic surgery (LAP) for colorectal cancer has not yet been fully evaluated in elderly patients. The aim of this study was to compare the short term surgical outcomes of LAP and evaluate the safety and feasibility of LAP in colorectal cancer patients aged > 75 years. Methods: This retrospective study enrolled consecutive patients who underwent laparoscopic surgery for colorectal cancer between April 2013 and March 2014 at Yokohama City University Hospital and its related general hospitals. The patients were categorized into two groups: elderly patients (≧75 years of age: group A) and non-elderly patients ( < 75 years of age: group B). Surgical outcomes and postoperative complications were compared between the two groups. The severity of complications was evaluated using the Clavien–Dindo classification. Results: A total of 237 patients were evaluated in the present study. Eighty-four patients were classified into group A, and 153 into group B. Preoperative clinicopathological outcomes demonstrated no significant differences except for the ASA score. When comparing the surgical outcomes between group A and group B, the rate of conversion to open procedure (3.6 % vs 5.2 %, P = 0.751), median operation time (232 min vs 232 min, P = 0.318), median blood loss (20 ml vs 12 ml, P = 0.353). There was no significant difference in the surgical outcomes. Although the incidence of Japanese D3 dissection was significantly lower in Group A (56 % vs 69.3 %, P = 0.047), the incidences of postoperative surgical complications of grade ≧ Ⅱ were similar between two groups (15.5 % vs 11.8 %, p = 0.427). The length of postoperative hospital stay was also similar (10days vs 10days, p = 0.347). Conclusions: The present study suggested that laparoscopic surgery for colorectal cancer is safe and feasible, regardless of the age of the patient, especially for elderly patients who may be candidates for colorectal cancer surgery.
29
Sutton, Damon, Shihu Wang, Norased Nasongkla, Jinming Gao, and Elena E. Dormidontova. "Doxorubicin and β-Lapachone Release and Interaction with Micellar Core Materials: Experiment and Modeling." Experimental Biology and Medicine 232, no. 8 (September 2007): 1090–99. http://dx.doi.org/10.3181/0702-rm-31.
Full textAbstract:
Polymer micelles with two different core-forming blocks, poly(d,l -lactide) (PLA) and poly(ε-caprolactone) (PCL), but the same coronal material, poly(ethylene glycol) (PEG), were investigated in this study as nanoscopic drug carriers. The release of two different drugs, doxorubicin (DOX) and β-lapachone (β-lap), from PEG(5k)-b-PCL(5k) and PEG(5k)-b-PLA(5k) micelles was studied at pH 5.0 and 7.4. Mathematical solutions of both Higuchi’s model and Fickian diffusion equations were utilized to elucidate the differences between the micelle core materials for the two drugs. The neutral and smaller of the two drugs tested, β-lap, demonstrated faster, pH-independent release, suggesting that no substantial changes occurred in either micelle core at lower pH. In contrast, the release rate of DOX was found to noticeably increase at lower pH with a larger cumulative amount of drug released. Different core materials were shown to have considerable influence on the release kinetics of both drugs: in both cases, the more hydrophobic PCL core showed slower drug release rates compared with the less hydrophobic PLA core.
30
Božić-Antić, Ivana, Dušan Ilić, Jelica Bjekić-Macut, Tamara Bogavac, Danijela Vojnović-Milutinović, Biljana Kastratovic-Kotlica, Nataša Milić, Olivera Stanojlović, Zoran Andrić, and Djuro Macut. "Lipid accumulation product as a marker of cardiometabolic susceptibility in women with different phenotypes of polycystic ovary syndrome." European Journal of Endocrinology 175, no. 6 (December 2016): 551–60. http://dx.doi.org/10.1530/eje-16-0775.
Full textAbstract:
AbstractObjectiveThere are limited data on cardiometabolic risk factors and the prevalence of metabolic syndrome (MetS) across the different PCOS phenotypes in Caucasian population. Lipid accumulation product (LAP) is a clinical surrogate marker that could be used for evaluation of MetS in clinical practice. The aim of the study was to analyze metabolic characteristics and the ability of LAP to predict MetS in different PCOS phenotypes.DesignCross-sectional clinical study analyzing 365 women with PCOS divided into four phenotypes according to the ESHRE/ASRM criteria, and 125 healthy BMI-matched controls.MethodsIn all subjects, LAP was determined and MetS was diagnosed according to the National Cholesterol Education Program/Adult Treatment Panel III (NCEP-ATP III), the International Diabetes Federation (IDF) and the Joint Interim Statement (JIS) criteria. Logistic regression and ROC curve analyses were used to determine predictors of MetS in each PCOS phenotype. All analyses were performed with age and BMI adjustment.ResultsAll PCOS phenotypes in comparison to controls had higher prevalence of MetS assessed by NCEP-ATP III criteria, and only classic phenotypes when IDF and JIS criteria were used. All phenotypes had the same prevalence of MetS irrespective of used definition. LAP and exhibited the highest diagnostic accuracy and was an independent predictor of MetS in all phenotypes.ConclusionLAP is an independent and accurate clinical determinant of MetS in all PCOS phenotypes in our Caucasian population. All PCOS phenotypes, including non-classic ones, are metabolically challenged and with cardiovascular risk, particularly phenotype B.
31
Arnaz, Ahmet, and Dilek Altun. "Monitoring Left Atrial Pressure With a Useful Epidural Catheter." Heart Surgery Forum 21, no. 4 (June 14, 2018): E250—E253. http://dx.doi.org/10.1532/hsf.1991.
Full textAbstract:
Background: Left atrial pressure (LAP) monitoring provides a valuable option for management of hemodynamic status in pediatric congenital heart surgery during the postoperative period.Methods: We used a Perifix Soft Tip 701 filter set catheter (B. Braun Melsungen AG 34209 Melsungen, Germany), which is commonly used for continuous epidural anesthesia. The catheter was placed into the left atrium to provide LAP monitoring. The study included 33 children, who needed LAP monitoring during and post-surgery between January 2016 and September 2017. Patients retrospectively were reviewed for ease of catheter placement, effectivity, accurate LAP, postoperative stability, and complications after removal of the catheter, including bleeding, air embolism, or thromboembolic events.Results: Patients ranged in age from 2 days to 131.27 months (Mean 11.80 days ± 25.76 days). Fifteen patients were newborn. Of the newborn patients, 39.4% (N = 13) were female. Catheters successfully were implanted in all patients, and there were no procedural complications. Left atrial catheters were removed between 1 to 12 days, with an average of 3.55 days ± 3.39 days before chest tube removal. During follow-up, patients did not suffer complications, such as thrombus formation, catheter-related infection, systemic embolism or stroke. Also, after the catheter was withdrawn, patients did not suffer from bleeding, haemodynamic deterioration, catheter retention, or require the need for intervention. During the stay in ICU, catheter nonfunction was the most frequent complication at 12.1 percent (N = 4). Conclusion: This is a simple, easy, and safe technique for wide use in congenital cardiac surgery patients.
32
Wojdat, Tomasz, Paweł Kustroń, Karol Jaśkiewicz, and Jarosław Pabian. "Study of Corrosion, Structural, and Mechanical Properties of EN AW-6082 and EN AW-7075 Welded Joints." Materials 14, no. 16 (August 4, 2021): 4349. http://dx.doi.org/10.3390/ma14164349.
Full textAbstract:
The purpose of the work was to test the welded joints of aluminum alloys EN AW-7075 and EN AW-6082, which are used to join individual structural elements of car bodies, e.g., B-pillar with the body. The joints were made using the low-energy cold metal transfer (CMT) arc welding method. The results of the structure investigations of lap and butt joints, as well as tests of mechanical properties are presented. The influence of linear energy and the way of arranging materials in lap joints on the possibility of hot cracks occurrence has been demonstrated. The shear strength of lap joints was equal to 150 MPa, while the tensile strength of butt joints was equal to 375 MPa. The highest hardness reduction was observed in the heat affected zone (HAZ) from the EN AW-7075 alloy side in the range of 98 to 138 HV 0.05. In addition, a significant reduction of the corrosion resistance in the transition zone between HAZ and the base material (EN AW-7075 alloy) in the medium salinity environment, corresponding to the sea conditions according to ASTM G85 was indicated.
33
Elbalshy, Mohammed A., Hossam Abdelkader El Fol, Mohamed S. Ammar, and Mahmoud Gamaleldeen Hagag. "Outcomes of laparoscopic assisted versus open complete mesocolic excision for right sided colon cancer." International Surgery Journal 6, no. 12 (November 26, 2019): 4203. http://dx.doi.org/10.18203/2349-2902.isj20195376.
Full textAbstract:
Background: Complete mesocolic excision (CME) as a standard surgery for left sided colon cancer is proven however there is a great debate and deal of discussion if the extra effort needed for it in right sided colon cancer worth the gain that is achieved. We aimed in our study to assess the outcome of CME with central vascular ligation for treatment of right sided colon cancer performed either laparoscopic (lap.) assisted or by open technique.Methods: This was a prospective randomized study on sixty patients who were presented with right sided colonic cancer. Patients were divided into two groups. Group (A) open one and group (B) lap. assisted group.Results: Both groups were comparable regarding demographic data, although operative time was significantly shorter in open group (p>0.001). Lap. assisted group achieved a significant difference as regards to blood loss, incision length, duration of hospital stay, resume of oral feeding, tumor to high vascular tie as well as higher number of harvested lymph nodes with no difference in recurrence rate and overall survival rate over a period of 3 years follow up.Conclusions: The principles underlying CME are anatomical and logical as it entails return back to embryology, and the results published points to the improved survival and decreased local recurrence particularly when it is performed in the proper mesocolic plane, using of laparoscopy may help in that due to better visualization of planes thus the gain of CME even in right sided colon cancer may exceed the pain in hand skilled colorectal lap. surgeons.
34
Carulli, Giovanni, Alessandra Marini, Ugo Baicchi, Paolo Simi, Federico Papineschi, and Fabio Ambrogi. "Chronic Lymphocytic Leukemia (B-Cell) in the Course of Polycythemia Vera. Description of a Case with an Unusual Chromosomic Anomaly." Tumori Journal 73, no. 6 (December 1987): 639–43. http://dx.doi.org/10.1177/030089168707300616.
Full textAbstract:
The present paper describes the case of a patient who developed a B-cell chronic lymphocytic leukemia (B-CLL) 15 months after the diagnosis of polycythemia vera, which had been treated only with phlebotomies. In spite of lymphocytosis and the clinical signs and symptoms of leukemia, the patient exhibited at the same time presumptive elements of polycythemia (high LAP index levels, a high number of neutrophils). Cytogenetic investigations, carried out after the appearance of B-CLL, revealed the presence of an unusual abnormality (18 p+) both in bone marrow not stimulated by mitogens and in PWM-stimulated circulating lymphocytes. This case, which is the ninth of its kind described in the literature, offers some interesting observations about the association between myeloproliferative and lymphoproliferative syndromes.
35
You, Min, Jia Ling Yan, Xiao Ling Zheng, Nan Feng Xiong, and Ding Feng Zhu. "The Effect of Adhesive for Fillet on Stress in Weld-Bonded Joints." Key Engineering Materials 450 (November 2010): 502–5. http://dx.doi.org/10.4028/www.scientific.net/kem.450.502.
Full textAbstract:
The influence of the fillet with different elastic modulus on the stress distributed in weld-bonded aluminum alloy single lap joint was investigated using elasto-plastic finite element method. The results show that the peak values of the stress along the mid-bondline at the points near the fillet edge were increased as the elastic modulus of the fillet increased. But at points near the both ends of the adherend in over lap zone as well as in the region of the nugget the peak stresses were decreased except longitudinal stress Sx. The peak value of Seqv decreased first, and then it increased again as the elastic modulus in fillet increased. The load-bearing capacity of the whole weld-bonded joints may be improved for the fillet with Adhesive B (825 MPa) for the relative high stress region in nugget was wider and the stress distribution in overlap zone was more uniform.
36
Foisner, Roland. "Cell Cycle Dynamics of the Nuclear Envelope." Scientific World JOURNAL 3 (2003): 1–20. http://dx.doi.org/10.1100/tsw.2003.06.
Full textAbstract:
The nuclear envelope (NE) consists of an inner and an outer membrane, nuclear pore complexes, and the underlying nuclear lamina, a filamentous scaffold structure formed by lamins. The inner membrane is linked to the lamina and chromatin by its integral membrane proteins, such as lamin B receptor (LBR), emerin, and various isoforms of lamina-associated polypeptides (LAP) 1 and 2, which bind lamins and/or chromatin. During mitosis, the NE is disassembled upon phosphorylation of its core components, and the NE is torn apart by a dynein-driven microtubule-dependent mechanism. Nuclear reassembly after sister chromatid separation requires a timely coordinated and dephosphorylation-dependent association of lamin-binding proteins and lamins with chromosomal proteins and targeting of membranes to specific sites on chromosomes. Various chromatin-binding domains in lamina proteins, such as the LEM domain, present in all LAP2 isoforms and in emerin, as well as unique regions in lamina proteins and in specific LAP2 isoforms have been implicated in defined steps of NE reformation. Furthermore, novel mechanisms of membrane fusion involving Ran GTPase are just beginning to emerge.
37
Souza, D. L., A. Evangelista-Rodrigues, M. N. Ribeiro, F. Padilla Álvarez, E. S. L. Farias, and W. E. Pereira. "Análises morfométricas entre apis mellifera da mesorregião do sertão paraibano." Archivos de Zootecnia 58, no. 221 (June 28, 2007): 65–71. http://dx.doi.org/10.21071/az.v58i221.5321.
Full textAbstract:
Objetivou-se, com esse trabalho, caracterizar as populações de Apis mellifera da mesorregião do Sertão paraibano, comparando os indivíduos morfometricamente. As medidas tomadas foram: comprimento da asa anterior (CAA) e largura da asa anterior (LAA), ângulos B4, E9, G18, J16, K19, N23, O26 e distância b (Db); comprimento da asa posterior (CAP) e largura da asa posterior (LAP), distância L2 (DL2), L5 (DL5) e ângulo W3, comprimento do fêmur (CF), comprimento da tíbia (CT), comprimento do tarso (CTS) e largura do tarso (LTS) e comprimento da probóscide (CP). Para a comparação das diferentes variáveis estudadas utilizou-se a análise de variância, o teste de Tukey, análise canônica e o teste de Tocher a 5% de significância. Para as medidas realizadas em cada amostra utilizou-se um equipamento de análise de imagens, Programa Image Pro Plus 4.0. As variáveis CTS e LTS, ângulos G18 e K19 e DL2 diferiram significativamente (p
38
Sato, Atsushi, Hideyo Hirai, Asumi Yokota, Akihiro Tamura, Tsukimi Shoji, Takahiro Kashiwagi, Masaki Iwasa, Aya Fujishiro, Yasuo Miura, and Taira Maekawa. "C/EBPβ Isoforms Distinctively and Collaboratively Regulate the Behavior of Hematopoietic Stem and Progenitor Cells in Regenerative Conditions." Blood 126, no. 23 (December 3, 2015): 3580. http://dx.doi.org/10.1182/blood.v126.23.3580.3580.
Full textAbstract:
Abstract CCAAT Enhancer Binding Protein b (C/EBPb) is a leucine zipper type transcription factor. While C/EBPa plays a critical role in maintaining steady-state granulopoiesis, C/EBPb is required for stress-induced granulopoiesis (Hirai et al., 2006). We have been focusing on the functions of C/EBPb in the regulation of hematopoietic stem and progenitor cells (HSPCs) especially under stressed conditions. Last year in this meeting, we have shown that 1) C/EBPb was upregulated at protein level in HSPCs after hematopoietic stresses, 2) C/EBPb was required for initial expansion of HSPCs after transplantation, and 3) C/EBPb promoted exhaustion of HSPCs under repetitive hematopoietic stresses (56th ASH, abstract #67850). Here, we further investigated the significance of C/EBPb in cell cycle regulation of HSPCs and the distinct roles of C/EBPb isoforms in HSPCs during regenerative conditions. To clarify the involvement of C/EBPb in cell cycle regulation of HSPCs, we compared the cell cycle status of wild-type (WT) and Cebpb knockout (KO) HSPCs by intracellular Ki67 staining and short-term BrdU incorporation assay in combination with multi-color flow cytometric analysis. In order to exclude the difference in the bone marrow microenvironment, CD45.2+ WT or Cebpb KO bone marrow (BM) cells were transplanted into lethally irradiated CD45.1+ WT mice. At steady state (12 weeks after the BM transplantation), the cell cycle status of Cebpb KO HSPCs was identical to that of WT HSPCs. Then cell cycle status of HSPCs was assessed at various time points during regeneration after intraperitoneal administration of 5-fluorouracil (5-FU, 150mg/kg). We found that significantly more Cebpb KO HSPCs remained in the G0 phase than WT HSPCs (in LT-HSCs on days 3-10; in MPPs on days 6-12). Significantly less Cebpb KO HSPCs were BrdU+ and were in the S/G2/M phase on day 7. These findings suggest that C/EBPb, in a cell-intrinsic manner, facilitates cell cycle entry, progression and consequent earlier expansion of HSPCs in response to hematopoietic stresses. Next, we investigated the distinct roles of C/EBPb isoforms in regulation of HSPCs. C/EBPb is a unique single exon gene and utilization of three different initiating codons result in three distinct isoforms. Liver-enriched activating protein* (LAP*) and LAP are the longer isoforms containing transactivating domains, DNA binding and dimerization domains, and liver-enriched inhibitory protein (LIP) is the shortest isoform which lacks the transactivating domains. In order to examine the expression pattern of C/EBPb isoforms in vivo in scarce populations of regenerating HSPCs, we developed a novel flow cytometric method to distinguish the cells predominantly expressing shorter isoform (LIP) from the cells expressing both LIP and the longer isoforms (LAP* and LAP) by intracellular double staining. Using this method, we found that predominantly LIP-expressing cells transiently emerged within MPP fraction in the regenerating bone marrow (on days 5-6 after administration of 5-FU, Figure below), while overall C/EBPb expression levels were significantly upregulated in most cells. To examine the roles of respective C/EBPb isoforms in regulation of HSPCs, EML cells, a murine hematopoietic stem cell line, were retrovirally transduced with one of the C/EBPb isoforms and the transduced cells were subjected to further analysis (vectors are kind gifts from Dr Watanabe-Okouchi N and Dr Kurokawa M, Tokyo Univ). LIP-expressing EML cells were more proliferative and actively cycling than EML cells transduced with a control vector, whereas the proliferation of LAP*- or LAP-expressing cells were markedly suppressed. LIP-expressing cells remained undifferentiated status (c-kithigh CD11b-) for more than 2 weeks, while LAP*- or LAP-expressing cells rapidly differentiated into c-kitlow CD11b+ myeloid cells and eventually exhausted within a week. These results indicate LIP plays quite distinct roles from LAP* and LAP in regulation of HSPCs. Collectively, our data suggest that C/EBPb isoforms distinctively and collaboratively regulate HSPCs in regenerative conditions: early transient elevation of LIP contributes to cell cycle activation and rapid expansion of HSPC population, which is in turn converted into supply of mature myeloid cells by more abundant upregulation of LAP* and LAP. Figure 1. Figure 1. Disclosures No relevant conflicts of interest to declare.
39
Farfán, R. A., J. A. Espíndola, M. I. Gomez, M. C. L. de Jiménez, M. A. Martínez, O. E. Piro, and E. E. Castellano. "Structural and Spectroscopic Properties of Two New Isostructural Complexes of Lapacholate with Cobalt and Copper." International Journal of Inorganic Chemistry 2012 (April 29, 2012): 1–6. http://dx.doi.org/10.1155/2012/973238.
Full textAbstract:
The molecular structures of two isostructural complexes of lapacholate (Lap) anion and dimethylformamide (DMF), M(Lap)2(DMF)2 with M: Co Cu, were determined by X-ray diffraction methods. The substances crystallize in the triclinic space group P1¯ with one molecule per unit cell and cell constants a=7.7591(3), b=10.3560(3), c=11.2224(4) Å, α=95.110(2), β=94.310(2), and γ=107.704(2)° for the Co complex and a=7.9308(2), b=10.0033(4), c=10.7508(4), α=97.387(2), β=93.621(2), and γ=103.980(2)° for the Cu complex. The structures were solved from 2933 (Co) and 2888 (Cu) reflections with I>2σ (I) and refined by full matrix least squares to agreement R1-factors of 0.041 (Co) and 0.033 (Cu). The metal M(II) ion is sited on a crystallographic inversion center in a MO6 distorted octahedral environment. This ion is coordinated equatorially to two lapacholate anions through their adjacent carbonyl and phenol oxygen atoms [M–O bond distances of 2.134(1) and 2.008(1) Å (Co) and 2.301(1) and 1.914(1) Å (Cu)] and axially to two DMF molecules through oxygen atoms [M–O bond lengths of 2.143(1) Å (Co) and 2.069(1) Å (Cu)]. The solid state IR transmittance and solution electronic absorption spectra of both Co and Cu compounds are also reported and compared to each other and to the corresponding spectra of other members of the lapacholate metal family of complexes.
40
Wu, Bing, Shui Li Gong, and Li Chen. "Stress Distribution Characteristics of Different Laser Welding Joints." Advanced Materials Research 146-147 (October 2010): 1863–69. http://dx.doi.org/10.4028/www.scientific.net/amr.146-147.1863.
Full textAbstract:
For quantitatively understanding the influences of the welded lap joint forms on the stress concentration, six different forms of overlap joints were designed, and then the stress distribution characteristics of these overlap joints were carried out in this paper. The results show that, the stress concentration of the model (a) and (b) are the highest level; and the stress concentration of the model (c) and (d) are significantly lower; the stress concentration of the model (e) is even lower than that of model (c) and (d); the stress concentration of the model (f) is zero.
41
Zhang, Yu, Richard Morgan, Chuan Chen, Yancheng Cai, Emily Clark, Wasif Noor Khan, Seung-Uon Shin, et al. "Mammary-tumor-educated B cells acquire LAP/TGF-β and PD-L1 expression and suppress anti-tumor immune responses." International Immunology 28, no. 9 (February 19, 2016): 423–33. http://dx.doi.org/10.1093/intimm/dxw007.
Full text
42
Fan, Sijia, Jing Wang, Guangqing Yu, Fangjing Rong, Dawei Zhang, Chenxi Xu, Juan Du, Zhi Li, Gang Ouyang, and Wuhan Xiao. "TET is targeted for proteasomal degradation by the PHD-pVHL pathway to reduce DNA hydroxymethylation." Journal of Biological Chemistry 295, no. 48 (September 22, 2020): 16299–313. http://dx.doi.org/10.1074/jbc.ra120.014538.
Full textAbstract:
Hypoxia-inducible factors are heterodimeric transcription factors that play a crucial role in a cell's ability to adapt to low oxygen. The von Hippel-Lindau tumor suppressor (pVHL) acts as a master regulator of HIF activity, and its targeting of prolyl hydroxylated HIF-α for proteasomal degradation under normoxia is thought to be a major mechanism for pVHL tumor suppression and cellular response to oxygen. Whether pVHL regulates other targets through a similar mechanism is largely unknown. Here, we identify TET2/3 as novel targets of pVHL. pVHL induces proteasomal degradation of TET2/3, resulting in reduced global 5-hydroxymethylcytosine levels. Conserved proline residues within the LAP/LAP-like motifs of these two proteins are hydroxylated by the prolyl hydroxylase enzymes (PHD2/EGLN1 and PHD3/EGLN3), which is prerequisite for pVHL-mediated degradation. Using zebrafish as a model, we determined that global 5-hydroxymethylcytosine levels are enhanced in vhl-null, egln1a/b-double-null, and egln3-null embryos. Therefore, we reveal a novel function for the PHD-pVHL pathway in regulating TET protein stability and activity. These data extend our understanding of how TET proteins are regulated and provide new insight into the mechanisms of pVHL in tumor suppression.
43
Hempel, Charlotte. "CD Manuscript B and the Rule of the Community—Reflections on a Literary Relationship." Dead Sea Discoveries 16, no. 3 (2009): 370–87. http://dx.doi.org/10.1163/156851709x473987.
Full textAbstract:
AbstractThis article begins by noting the proliferation of textual overlap between the Damascus Document and the Rule of the Community. Some examples of such over-lap, such as the penal code, have received a large amount of scholarly attention in the wake of the publication of all the Cave 4 manuscripts. This study returns to a passage from CD manuscript B (CD 20:1b‐8a) that has long been recognized as closely related to 1QS 8‐9 and offers a reconsideration of the relationship between both texts in the light of the full publication of all the S manuscripts. The analysis offered here uncovers a complex inter-relationship between both texts as well as some intriguing pointers towards a complex literary history within the S tradition.
44
Martins, Sandra, Sissel Eikvar, Kazuhiro Furukawa, and Philippe Collas. "HA95 and LAP2β mediate a novel chromatin–nuclear envelope interaction implicated in initiation of DNA replication." Journal of Cell Biology 160, no. 2 (January 20, 2003): 177–88. http://dx.doi.org/10.1083/jcb.200210026.
Full textAbstract:
HA95 is a chromatin-associated protein that interfaces the nuclear envelope (NE) and chromatin. We report an interaction between HA95 and the inner nuclear membrane protein lamina-associated polypeptide (LAP) 2β, and a role of this association in initiation of DNA replication. Precipitation of GST–LAP2β fusion proteins and overlays of immobilized HA95 indicate that a first HA95-binding region lies within amino acids 137–242 of LAP2β. A second domain sufficient to bind HA95 colocalizes with the lamin B–binding domain of LAP2β at residues 299–373. HA95–LAP2β interaction is not required for NE formation. However, disruption of the association of HA95 with the NH2-terminal HA95-binding domain of LAP2β abolishes the initiation, but not elongation, of DNA replication in purified G1 phase nuclei incubated in S-phase extract. Inhibition of replication initiation correlates with proteasome-mediated proteolysis of Cdc6, a component of the prereplication complex. Rescue of Cdc6 degradation with proteasome inhibitors restores replication. We propose that an interaction of LAP2β, or LAP2 proteins, with HA95 is involved in the control of initiation of DNA replication.
45
del Villar Pérez, Víctor Manuel, Alberto Barreras Serrano, Lourdes Carolina Pujol Manríquez, Luis Tinoco Gracia, Tonatiuh Melgarejo, and Alma Rossana Tamayo Sosa. "Expression of b-defensins LAP (lingual antimicrobial peptide) and TAP (antimicrobial peptide tracheal), and Psoriasin (S100A7), in bovine mammary gland with chronic mastitis by Staphylococcus aureus." Acta Universitaria 26, no. 4 (September 2016): 29–35. http://dx.doi.org/10.15174/au.2016.930.
Full text
46
Zhao, Yadong, Yalong Luo, Haixiao Zhang, Xuefeng Guo, Shuguang Wang, Hongbao Cui, Yangming Zhang, Zhigang Wang, and Yafang Zhai. "Dissimilar Friction Stir Lap Welding of Al to Mg: Characteristic Signal, Microstructure and Mechanical Properties." Coatings 11, no. 1 (December 25, 2020): 19. http://dx.doi.org/10.3390/coatings11010019.
Full textAbstract:
The spindle characteristic signal (forces and vibrations) at different friction stir lap welding (FSLW) parameters were studied. The result indicated that the spindle force and vibration have different trends with the change of welding parameters. For further study, the spindle dynamic performance evaluation model by means of the Technique for Order Preference by Similarity to the Ideal Solution (TOPSIS method) was established. The model was used to calculate the relative approach degree B under different welding process parameters. The correlation between the characteristic signal and the joint properties was obtained. The model was validated by mechanical performance testing and microscopic observation. The results showed that the model evaluations were consistent with the experimental results.
47
Jacobs, J. P., K. J. Murray, C. J. Schultz, J. F. Wilson, M. S. Goswitz, C. W. Stevens, and J. D. Cox. "Central lymphatic irradiation for stage III nodular malignant lymphoma: long-term results." Journal of Clinical Oncology 11, no. 2 (February 1993): 233–38. http://dx.doi.org/10.1200/jco.1993.11.2.233.
Full textAbstract:
PURPOSE To report the long-term results of central lymphatic irradiation for stage III nodular malignant lymphoma. PATIENTS AND METHODS Between 1969 and 1985, 34 patients (26 with nodular poorly differentiated lymphoma, four with nodular mixed lymphocytic/histiocytic lymphoma, and four with nodular histiocytic lymphoma) were treated with central lymphatic irradiation. Median age of the group was 51 years (range, 30 to 73). There were 15 men and 19 women. Staging work-up included a physical examination and bone marrow biopsy in all patients. Seventy-four percent had a lymphangiogram (LAG) and 44% a laparotomy (LAP). Eighty-two percent had either a LAP or a LAG. Thirty-two patients were Ann Arbor stage IIIA and two were stage IIIB. All patients received lymphatic irradiation that encompassed cervical, supraclavicular, axillary, mediastinal, paraaortic, mesenteric, pelvic, and femoral lymphatics to total doses ranging from 20 to 30 Gy in 1.0- to 1.8-Gy fractions. Waldeyer's ring was initially treated in 17 patients. Follow-up information is available on all 34 patients. Median follow-up is 9 years, 8 months (range, 15 to 244 months). RESULTS Life-table actuarial overall, disease-free, and cause-specific survival rates at 15 years are 28%, 40%, and 46%, respectively. Only one relapse was observed after 9 years. Disease-free survival was significantly improved in patients with five or fewer sites of involvement (P = .02). Age, sex, B symptoms, histology, and technique of irradiation were not prognostically significant. Salvage therapy, including further irradiation and/or chemotherapy, was delivered to 20 patients. Ten percent of these patients remain alive without evidence of disease. Toxicity data were available for the patients treated at the Medical College of Wisconsin (MCW). Radiation Therapy Oncology Group (RTOG) acute hematologic, gastrointestinal, and salivary toxicity scores were < or = 2 in 83% of patients. Late toxicity scores were < or = 2 in 96%. Persistent xerostomia was noted in 23% of patients who received initial treatment to Waldeyer's ring. CONCLUSION These results suggest that initial comprehensive central lymphatic irradiation may be the preferred approach to achieve a durable relapse-free interval for this group of patients.
48
Rossi, Patrizia, Giovanni Giuseppe Vendramin, and Raffaello Giannini. "Estimation of mating system parameters in two Italian natural populations of Fagussylvatica." Canadian Journal of Forest Research 26, no. 7 (July 1, 1996): 1187–92. http://dx.doi.org/10.1139/x26-132.
Full textAbstract:
Mating system parameters were estimated in two Italian natural populations of European beech (Fagussylvatica L.) using a mixed mating model and considering seven allozyme loci (Idh-A, Lap-A, Mdh-B, Pgd-A, Pgd-B, Pgd-C, Skd-A). High values of multilocus estimates of the outcrossing rate were found in both populations, ranging from 94 to 98%. Comparison of single- and multi-locus estimates of outcrossing rates seems to indicate the presence of consanguineous matings, probably because the populations are substructured. This hypothesis seems to be confirmed by the presence of a heterogeneity of the pollen allele frequencies across female parent trees and by the significant coefficient of the regression of pollen allele frequencies on ovule genotype. Variation in the fixation indices in different life-cycle phases was observed, indicating possible presence of selective processes between seed set and sexual maturity. Possible explanations of these results are presented.
49
Martins, S. B., T. Eide, R. L. Steen, T. Jahnsen, S. Skalhegg B, and P. Collas. "HA95 is a protein of the chromatin and nuclear matrix regulating nuclear envelope dynamics." Journal of Cell Science 113, no. 21 (November 1, 2000): 3703–13. http://dx.doi.org/10.1242/jcs.113.21.3703.
Full textAbstract:
We report a role for HA95, a nuclear protein with high homology to the nuclear A-kinase anchoring protein AKAP95, in the regulation of nuclear envelope-chromatin interactions. Biochemical and photobleaching data indicate that HA95 is tightly associated with chromatin and the nuclear matrix/lamina network in interphase, and bound to chromatin at mitosis. HA95 resides in a complex together with lamin B receptor (LBR), lamina-associated polypeptide (LAP)2 and emerin, integral proteins of the inner nuclear membrane. Cross-linking experiments, however, illustrate a tight association of HA95 with LBR and LAP2 only. Intra-nuclear blocking of HA95 with anti-HA95 antibodies abolishes nuclear breakdown in a mitotic HeLa cell extract. The antibodies inhibit nuclear membrane breakdown and chromatin condensation - the latter independently of nuclear membranes. However, lamina disassembly is not affected, as judged by immunological analyses of A/C- and B-type lamins. In contrast, immunoblocking of HA95 bound to condensed chromosomes does not impair chromatin decondensation, nuclear membrane reassembly or lamina reformation. Our results argue for a role for HA95 in anchoring nuclear membranes and lamins to chromatin in interphase, and in releasing membranes from chromatin at mitosis. The data also suggest that HA95 is not involved in initial binding of membranes to chromatin upon nuclear reassembly. We propose that HA95 is a central platform at the chromatin/nuclear matrix interface implicated in regulating nuclear envelope-chromatin interactions during the cell cycle.
50
Lopes, Patricia P., George Todorov, Thanh T. Pham, Anthony B. Nesburn, Elmostafa Bahraoui, and Lbachir BenMohamed. "Laser Adjuvant-Assisted Peptide Vaccine Promotes Skin Mobilization of Dendritic Cells and Enhances Protective CD8+TEMand TRMCell Responses against Herpesvirus Infection and Disease." Journal of Virology 92, no. 8 (February 7, 2018): e02156-17. http://dx.doi.org/10.1128/jvi.02156-17.
Full textAbstract:
ABSTRACTThere is an urgent need for chemical-free and biological-free safe adjuvants to enhance the immunogenicity of vaccines against widespread viral pathogens, such as herpes simplex virus 2 (HSV-2), that infect a large proportion of the world human population. In the present study, we investigated the safety, immunogenicity, and protective efficacy of a laser adjuvant-assisted peptide (LAP) vaccine in the B6 mouse model of genital herpes. This LAP vaccine and its laser-free peptide (LFP) vaccine analog contain the immunodominant HSV-2 glycoprotein B CD8+T cell epitope (HSV-gB498–505) covalently linked with the promiscuous glycoprotein D CD4+T helper cell epitope (HSV-gD49–89). Prior to intradermal delivery of the LAP vaccine, the lower-flank shaved skin of B6 or CD11c/eYFP transgenic mice received a topical skin treatment with 5% imiquimod cream and then was exposed for 60 s to a laser, using the FDA-approved nonablative diode. Compared to the LFP vaccine, the LAP vaccine (i) triggered mobilization of dendritic cells (DCs) in the skin, which formed small spots along the laser-treated areas, (ii) induced phenotypic and functional maturation of DCs, (iii) stimulated long-lasting HSV-specific effector memory CD8+T cells (TEMcells) and tissue-resident CD8+T cells (TRMcells) locally in the vaginal mucocutaneous tissues (VM), and (iv) induced protective immunity against genital herpes infection and disease. As an alternative to currently used conventional adjuvants, the chemical- and biological-free laser adjuvant offers a well-tolerated, simple-to-produce method to enhance mass vaccination for widespread viral infections.IMPORTANCEHerpes simplex viruses 1 and 2 (HSV-1 and HSV-2) infect a large proportion of the world population. There is an urgent need for chemical-free and biological-free safe adjuvants that would advance mass vaccination against the widespread herpes infections. The present study demonstrates that immunization with a laser-assisted herpes peptide vaccine triggered skin mobilization of dendritic cells (DCs) that stimulated strong and long-lasting HSV-specific effector memory CD8+T cells (TEMcells) and tissue-resident CD8+T cells (TRMcells) locally in the vaginal mucocutaneous tissues. The induced local CD8+T cell response was associated with protection against genital herpes infection and disease. These results draw attention to chemical- and biological-free laser adjuvants as alternatives to currently used conventional adjuvants to enhance mass vaccination for widespread viral infections, such as those caused by HSV-1 and HSV-2.