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1

Duarte, Sabrina Castilho, Juliana Alves Parente, Maristela Pereira, Célia Maria de Almeida Soares, and Guido Fontgalland Coelho Linhares. "Phylogenetic characterization of Babesia canis vogeli in dogs in the state of Goiás, Brazil." Revista Brasileira de Parasitologia Veterinária 20, no. 4 (2011): 274–80. http://dx.doi.org/10.1590/s1984-29612011000400004.

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The genus Babesia comprises protozoa that cause diseases known as babesiosis. Dogs are commonly affected by Babesia canis or Babesia gibsoni. Babesia canis is divided into the subspecies Babesia canis canis, Babesia canis vogeli and Babesia canis rossi. Among these, Babesia canis vogeli predominates in Brazil. The objective of this study was to conduct a phylogenetic analysis on Babesia isolates from dogs in Goiânia, Goiás. Blood samples were obtained from 890 dogs presenting clinical signs suggestive of canine babesiosis that were attended at a veterinary hospital of Goiás. Only samples prese
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2

Mihaljica, D., Z. Radulovic, Snezana Tomanovic, Sanja Cakic, Aleksandra Penezic, and Marija Milutinovic. "Molecular detection of Babesia spp. in ticks in northern Serbia." Archives of Biological Sciences 64, no. 4 (2012): 1591–98. http://dx.doi.org/10.2298/abs1204591m.

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In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pancevacki Rit, Titov Gaj, Makis, PKB and Kljajicevo. Prev
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3

Nolte, I., and Jennifer Jensen. "Autochthone Babesia-canis-Infektion bei einem Hund aus Norddeutschland." Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere 33, no. 06 (2005): 408–12. http://dx.doi.org/10.1055/s-0037-1622499.

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Zusammenfassung Gegenstand und Ziel: Beschreibung einer autochthonen Babesia-canis-Infektion in Norddeutschland. Untersuchungsgut, Material und Methode: Fallbericht über einevier Jahre alte Mischlingshündin. Die Diagnose erfolgte mittels Direktnachweis von großen Babesien in Erythrozyten. Ergebnisse: Die Hündin hatte Norddeutschland nie verlassen. Sie zeigte eine schwere perakute bis akute Erkrankung und verstarb an der Infektion. Schlussfolgerungen: Endemische Gebiete für Babesia canis in Deutschland sind für einige Regionen Süddeutschlands bekannt. Für Norddeutschland liegen bisher keine Ber
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4

Cardoso, Luís, Álvaro Costa, Joana Tuna, et al. "Babesia canis canis and Babesia canis vogeli infections in dogs from northern Portugal." Veterinary Parasitology 156, no. 3-4 (2008): 199–204. http://dx.doi.org/10.1016/j.vetpar.2008.05.027.

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5

Brkljačić, Mirna, Vesna Matijatko, Ivana Kiš, et al. "Molecular evidence of natural infection with Babesia canis canis in Croatia." Acta Veterinaria Hungarica 58, no. 1 (2010): 39–46. http://dx.doi.org/10.1556/avet.58.2010.1.4.

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The aim of the present study was to detect and characterise the species and subspecies of Babesia spp. that cause canine babesiosis in Croatia. Twenty-eight dogs with typical signs of babesiosis (lethargy, anorexia, fever, dark urine and thrombocytopenia) were included in this study. Their blood smears showed the presence of Babesia canis . The results showed the detection of one subspecies, namely Babesia canis canis using PCR, and subsequent sequence analysis demonstrated portions of the nss rRNA gene in 27 out of 28 samples. Sequence analysis of the isolates showed 100% identity in 11 sampl
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6

Demeter, Zoltán, Elena Palade, Éva Balogh, et al. "Postmortem small babesia-like morphology of Babesia canis — Short communication." Acta Veterinaria Hungarica 59, no. 4 (2011): 427–32. http://dx.doi.org/10.1556/avet.2011.029.

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Here we report a case of canine babesiosis with unusual morphology of the causative agent. A male, seven-week-old Labrador retriever puppy, exhibiting severe anaemia and haemoglobinuria, was presented at the Clinic of Internal Medicine in February 2011. The puppy was euthanised. The most relevant pathological changes were icterus, severe splenomegaly, generalised lymphadenopathy and haemoglobin nephrosis. Samples were collected from various organs for histology within one hour post mortem. Impression smears were also prepared from the spleen after overnight storage at 4 °C. Tissue sections and
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7

Ciuca, Lavinia, Gabriela Martinescu, Liviu Dan Miron, et al. "Occurrence of Babesia Species and Co-Infection with Hepatozoon canis in Symptomatic Dogs and in Their Ticks in Eastern Romania." Pathogens 10, no. 10 (2021): 1339. http://dx.doi.org/10.3390/pathogens10101339.

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Although the distribution of Babesia spp. and Hepatozoon canis is well known in Romania, there is still a marked lack of information in many places of the country. This study aimed to investigate the occurrence of these haemoparasites in symptomatic dogs and in their ticks in Iasi, eastern Romania. Ninety owned dogs were subjected to clinical examination at the Faculty of Veterinary Medicine of Iasi and all detectable ticks (58 ticks from 15 dogs) were collected. Additionally, 124 ticks collected from the coat of other dogs (no. = 23) were included. Three Babesia species were found in dogs: Ba
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8

Panait, Luciana Cătălina, Kristýna Hrazdilová, Angela Monica Ionică, et al. "Babesia pisicii n. sp. and Babesia canis Infect European Wild Cats, Felis silvestris, in Romania." Microorganisms 9, no. 7 (2021): 1474. http://dx.doi.org/10.3390/microorganisms9071474.

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Haemoparasites of the genus Babesia infect a wide range of domestic and wild animals. Feline babesiosis is considered endemic in South Africa, while data on Babesia spp. infection in felids in Europe is scarce. Using samples from 51 wild felids, 44 Felis silvestris and 7 Lynx lynx, the study aimed to determine the presence and genetic diversity of Babesia spp. in wild felids in Romania by analyzing the 18S rDNA and two mitochondrial markers, cytochrome b (Cytb) and cytochrome c oxidase subunit I (COI) genes. By 18S rDNA analyses, Babesia spp. DNA was detected in 20 European wild felids. All se
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9

Pawełczyk, Olga, Damian Kotela, Marek Asman, et al. "The First Records of Canine Babesiosis in Dogs from Dermacentor reticulatus—Free Zone in Poland." Pathogens 11, no. 11 (2022): 1329. http://dx.doi.org/10.3390/pathogens11111329.

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Tick-borne microorganisms belong to important etiological agents of many infectious diseases affecting humans and animals. Among them, there are haemoprotozoans of the Babesia genus, which infect erythrocytes of a host and may cause many clinical symptoms. Canine babesiosis is an emerging tick-borne disease in Southern and Central Europe. In this study, we report two cases of symptomatic canine babesiosis caused by Babesia canis in domestic dogs from the Silesian Voivodeship, Poland, as well as the presence of Dermacentor reticulatus ticks detected on one of the Babesia-infected dogs (D. retic
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10

Загуменнов, А. В., and И. Д. Генгин. "STATISTICS OF DOGS BABESIOSIS SPREADING IN CONDITIONS OF SCIENTIFIC AND EDUCATIONAL CENTER OF VETERINARY MEDICINE AND ITS THERAPY FOR THE PERIOD 2021–2022." Bulletin of Izhevsk State Agricultural Academy, no. 1(77) (March 29, 2024): 49–56. http://dx.doi.org/10.48012/1817-5457_2024_1_49-56.

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Бабезиоз (пироплазмоз) собак является насущной проблемой в Евразии и Америке для владельцев животных, а также ветеринарных врачей и фельдшеров. Сотни тысяч собак во всем мире ежегодно инвазируются бабезиями и распространяют их посредством промежуточных хозяев паразита. Бабезиоз можно отнести к заболеваниям средней тяжести, он относительно тяжело поддается лечению, и нередки случаи перехода в хроническую форму. В статье представлена эпизоотологическая статистика распространения кровепаразитов Babesia Canis и Babesia Gibsoni в Железнодорожном районе г. Пензы за период 2021 и 2022 гг., сравнитель
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11

Oyamada, Maremichi, Bernard Davoust, Mickaël Boni, et al. "Detection of Babesia canis rossi, B. canis vogeli, and Hepatozoon canis in Dogs in a Village of Eastern Sudan by Using a Screening PCR and Sequencing Methodologies." Clinical Diagnostic Laboratory Immunology 12, no. 11 (2005): 1343–46. http://dx.doi.org/10.1128/cdli.12.11.1343-1346.2005.

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ABSTRACT Babesia and Hepatozoon infections of dogs in a village of eastern Sudan were analyzed by using a single PCR and sequencing. Among 78 dogs, 5 were infected with Babesia canis rossi and 2 others were infected with B. canis vogeli. Thirty-three dogs were positive for Hepatozoon. Hepatozoon canis was detected by sequence analysis.
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12

Cacciò, Simone M., Boris Antunovic, Annabella Moretti, et al. "Molecular characterisation of Babesia canis canis and Babesia canis vogeli from naturally infected European dogs." Veterinary Parasitology 106, no. 4 (2002): 285–92. http://dx.doi.org/10.1016/s0304-4017(02)00112-7.

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13

Duarte, Sabrina Castilho, Guido Fontgalland Coelho Linhares, Tatiana Nunes Romanowsky, Osvaldo José da Silveira Neto, and Ligia Miranda Ferreira Borges. "Assessment of primers designed for the subspecies-specific discrimination among Babesia canis canis, Babesia canis vogeli and Babesia canis rossi by PCR assay." Veterinary Parasitology 152, no. 1-2 (2008): 16–20. http://dx.doi.org/10.1016/j.vetpar.2007.12.013.

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14

Грубіч, П. Ю., А. Ф. Курман, Л. В. Лепета та Є. А. Пархоменко. "Розробка ПЛР тест-системи для видової ідентифікації збудників бабезіозу тварин". Вісник Полтавської державної аграрної академії, № 2 (28 червня 2013): 98–101. http://dx.doi.org/10.31210/visnyk2013.02.27.

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Розроблена система олігонуклеотидних праймерів, що дозволяє ампліфікувати в ПЛР ділянки гену 18S рРНК 6 видів роду Babesia. Наведено особливостіконструювання праймерів та випробування мультиплексної ПЛР тест-системи для ідентифікації представників роду Babesia. Визначені довжини ампліфікованих фрагментів – від 299 до 258 пар нуклеотидів для Babesia canis, Babesia divergens, Babesia caballi, Babesia major, Babesia bovis. Досліджено 342 зразки крові від різних видів тварин і встановлено 100 % збіг із результатами мікроскопічних досліджень.
 The system of oligonucleotide primers that allow P
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15

AZMI, KIFAYA, AMER AL-JAWABREH, ABEDELMAJEED NASEREDDIN, et al. "Detection and molecular identification of Hepatozoon canis and Babesia vogeli from domestic dogs in Palestine." Parasitology 144, no. 5 (2016): 613–21. http://dx.doi.org/10.1017/s0031182016002201.

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SUMMARYDogs serve as hosts for a great number of parasites, which may affect their health and wellbeing. This study aimed to observe tick borne pathogens in dogs from Palestine including Hepatozoon canis and Babesia species. The prevalence of both H. canis and Babesia species infections in apparently healthy dogs, from ten districts of the West Bank was surveyed. DNA was extracted from blood samples obtained from dogs (n = 362) and ticks (n = 213) collected from dogs (n = 77). A primer set that amplifies a partial sequence of the Babesia and Hepatozoon 18S rRNA gene was used for PCR and the DN
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16

Spolidorio, Mariana Granziera, Mariana de Medeiros Torres, Wilma Neres da Silva Campos, et al. "Molecular detection of Hepatozoon canis and Babesia canis vogeli in domestic dogs from Cuiabá, Brazil." Revista Brasileira de Parasitologia Veterinária 20, no. 3 (2011): 253–55. http://dx.doi.org/10.1590/s1984-29612011000300015.

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The objective of this study was to report for the first time infection by Hepatozoon spp. and Babesia spp. in 10 dogs from the city of Cuiabá, State of Mato Grosso, central-western Brazil. A pair of primers that amplifies a 574 bp fragment of the 18S rRNA of Hepatozoon spp., and a pair of primers that amplifies a 551 bp fragment of the gene 18S rRNA for Babesia spp. were used. Six dogs were positive for Babesia spp., and 9 were positive for Hepatozoon spp. Co-infection of Babesia spp. and Hepatozoon spp. was seen in 5 dogs. Sequenced samples revealed 100% identity with B. canis vogeli, and H.
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17

Silva Júnior, Edilson, Adriana Cortez, Renata Bottura, Simone Gonçalves Rodrigues Gomes, Rafael Garabet Agopian, and Jonas Moraes Filho. "Diagnóstico molecular para hemoparasitos em cães atendidos no Hospital Veterinário da Universidade Santo Amaro, São Paulo, SP, Brasil." Brazilian Journal of Global Health 1, no. 3 (2021): 7–11. http://dx.doi.org/10.56242/globalhealth;2021;1;3;7-11.

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OBJECTIVE: Detection of Ehrlichia canis, Rickettsia rickettsii, Anaplasma platys, Rangelia vitalii, and Babesia canis vogeli using real-time PCR in dogs treated at the veterinary hospital of the Universidade Santo Amaro, located in the south zone, in the city of São Paulo, SP, Brazil. METHODS: DNA was extracted from 63 whole blood samples using the “PureLink Genomic DNA” extraction kit (Invitrogen®) according to the manufacturer's instructions and real-time PCR was performed to detect Ehrlichia canis, Rickettsia rickettsii, Anaplasma platys, Rangelia vitalii, and Babesia canis vogeli. RESULTS:
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18

Kelly, Patrick J., and Helene Lucas. "Failure to demonstrate Babesia, Anaplasma or Ehrlichia in thrombocytopenic dogs from St Kitts." Journal of Infection in Developing Countries 3, no. 07 (2009): 561–63. http://dx.doi.org/10.3855/jidc.475.

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Background: Thrombocytopenia is common in dogs on St Kitts but there is no data on the possible etiological role played by infections with Anaplasma, Babesia and Ehrlichia, other than E. canis, which are known to occur in the Caribbean. Methodology: Blood from 13 thrombocytopenic but apparently healthy dogs seronegative (Snap 3Dx) for E. canis were tested by PCR for Ehrlichia, Anaplasma and Babesia . Results: All PCRs were negative. Conclusions: The results confirm the high sensitivity of SNAP testing for E. canis and indicate Anaplasma and Babesia are not important causes of thrombocytopenia
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19

Mitkova, B., K. Hrazdilova, M. Novotna, et al. "Autochthonous Babesia canis, Hepatozoon canis and imported Babesia gibsoni infection in dogs in the Czech Republic." Veterinární Medicína 62, No. 3 (2017): 138–46. http://dx.doi.org/10.17221/152/2016-vetmed.

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The epidemiology of tick-borne diseases as well as the ecology of the associated tick vectors is currently undergoing dynamic change. Canine babesiosis has, to date, only been reported in the Czech Republic as an imported disease, despite the presence of an established population of D. reticulatus in the South Moravian region. A recent report of autochthonous H. canis in dogs indicates that the spectrum of tick-borne diseases in dogs in the Czech Republic may be broader than previously expected and that a survey of a large set of dogs may be required. The aim of the present study was to survey
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20

Mokryi, Yu O., I. M. Ksyonz, P. Yu Grubich, P. O. Kasala та O. M. Lysak. "Індикація та видова диференціація найпростіших роду Babesia за методом ПЛР у кліщах, знятих з тварин". Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 19, № 78 (2017): 99–103. http://dx.doi.org/10.15421/nvlvet7820.

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The article presents the study results on the indication and species differentiation of the Babesia genus protozoa in the organism of ticks taken off dogs and cattle. Diagnostic tests were performed using a multiplex PCR test system, being a self-engineering product, which allows to determine the DNA presence of 6 Babesia genus species in any biological samples, namely: B. canis, B. divergens, B. caballi, B. major, B Bigemina, B. bovis, three of them being species-specific, by the bands’ size at the amplification products’ electrophoregrams. The above test system contains 2 direct and 3 revers
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21

Carret, C., F. Walas, N. Grande, et al. "Bebesia canis canis and Babesia canis rossi : two different species?" Parasitology International 47 (August 1998): 285. http://dx.doi.org/10.1016/s1383-5769(98)80797-6.

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22

Ebani, Valentina Virginia, Chiara Trebino, Lisa Guardone, et al. "Occurrence of Bacterial and Protozoan Pathogens in Red Foxes (Vulpes vulpes) in Central Italy." Animals 12, no. 20 (2022): 2891. http://dx.doi.org/10.3390/ani12202891.

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Most surveys of pathogens in red foxes (Vulpes vulpes) have focused on particular agents. The aim of this study was to verify, with bacteriological and molecular analyses, the occurrence of the main bacterial and protozoan pathogens that are able to infect canids, in red foxes regularly hunted in Central Italy. Spleen, brain, kidney and fecal samples from red foxes were submitted to bacteriological and/or molecular analyses to detect Salmonella spp., Yersinia spp., Listeria monocytogenes, Brucella spp., Ehrlichia canis, Anaplasma phagocytophilum, Coxiella burnetii, Leptospira spp., Neospora ca
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23

Gottlieb, Juliana, Marcos Rogério André, João Fábio Soares, et al. "Rangelia vitalii, Babesia spp. and Ehrlichia spp. in dogs in Passo Fundo, state of Rio Grande do Sul, Brazil." Revista Brasileira de Parasitologia Veterinária 25, no. 2 (2016): 172–78. http://dx.doi.org/10.1590/s1984-29612016041.

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Abstract Pathogens transmitted by ticks are an emerging problem worldwide, this study aimed to diagnose the causal agents of infection in dogs presenting suspected hemoparasitoses. Fifty-eight dogs with clinical signs such as depression, hemorrhagic diathesis and fever were evaluated regarding clinical presentation, hemogram, blood smears and serological tests, using the indirect immunofluorescence method for the agents Babesia vogeli and Ehrlichia canis and conventional PCR for Babesia spp. (gene 18S rRNA), Rangelia vitalii (gene 18S rRNA) and Ehrlichia spp. (gene dsb). Five (8.6%) of the 58
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Badawi, Naseir Mohammed, and Afaf Abdulrahman Yousif. "Babesia canis spp. in dogs in Baghdad Province, Iraq: First molecular identification and clinical and epidemiological study." Veterinary World 13, no. 3 (2020): 579–85. http://dx.doi.org/10.14202/vetworld.2020.579-585.

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Aim: The aim of this study was to investigate babesiosis in dogs of different breeds and ages and of both sexes in Baghdad Province by molecular detection of Babesia canis using conventional polymerase chain reaction (PCR) and sequencing followed by phylogenetic analyses. Materials and Methods: Blood samples were collected from 310 dogs of different ages and breeds, and of both sexes in different areas of Baghdad Province from December 2018 to September 2019; during clinical examinations, body temperature, pulse, respiratory rate, and signs of diseases were recorded. PCR was used to amplify a
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Bilgic, Huseyin Bilgin, Gulcan Kırlı Pekel, Murat Hosgor, and Tulin Karagenc. "A Retrospective Epidemiological Study: The Prevalence of Ehrlichia canis and Babesia vogeli in Dogs in the Aegean Region of Turkey." Acta Veterinaria 69, no. 2 (2019): 164–76. http://dx.doi.org/10.2478/acve-2019-0013.

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Abstract Among tick-borne diseases, Ehrlichia canis and Babesia piroplasm cause important diseases in dogs where the distributions of the pathogen, vector and host overlap. The primary aim of the present study was to detect the prevalence of Babesia spp. and E. canis using PCR and reverse line blot (RLB) hybridization assay in a total of 379 samples comprising stray and owned dogs and to compare the diagnostic sensitivity of the two tests. Overall, 41.4% of dogs were infected with B. vogeli and/or E. canis as single (35.4%) and mixed (6.1%) infections. The majority of Babesia positive dogs (74
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CARRET, CÉLINE, FABIEN WALAS, BERNARD CARCY, et al. "Babesia Canis Canis, Babesia Canis Vogeli, Babesia Canis Rossi: Differentiation of the Three Subspecies By A Restriction Fragment Length Polymorphism Analysis On Amplified Small Subunit Ribosomal Rna Genes." Journal of Eukaryotic Microbiology 46, no. 3 (1999): 298–301. http://dx.doi.org/10.1111/j.1550-7408.1999.tb05128.x.

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27

Vinclérová, Veronika, Csilla Tóthová, and Viera Karaffová. "Uncomplicated canine babesiosis without prophylaxis: Case report and retrospective analysis." Folia Veterinaria 69, no. 2 (2025): 20–27. https://doi.org/10.2478/fv-2025-0014.

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Abstract Canine babesiosis is a serious disease caused by intraerythrocytic protozoa of the genus Babesia, most commonly Babesia canis and Babesia gibsoni. A four-year-old male Yorkshire Terrier was presented to a private veterinary clinic with anorexia, fever, weakness, vomiting, and hematuria. Microscopic examination of peripheral blood revealed the presence of Babesia canis. Further laboratory findings indicated marked thrombocytopenia and elevated total bilirubin levels, suggesting a severe clinical course potentially associated with hematopoietic dysfunction. The aim of this study was to
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Yamane, I., I. A. Gardner, C. P. Ryan, M. Levy, J. Urrico, and P. A. Conrad. "Serosurvey of Babesia canis, Babesia gibsoni and Ehrlichia canis in pound dogs in California, USA." Preventive Veterinary Medicine 18, no. 4 (1994): 293–304. http://dx.doi.org/10.1016/0167-5877(94)90054-x.

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BITTENCOURT, JOYCE, EMY HIURA, Samilla Alves Sobral, et al. "OCORRÊNCIA DE Babesia sp., Ehrlichia canis E Hepatozoon canis EM CÃES DOMICILIADOS, EM DOIS MUNICÍPIOS DO ESTADO DO ESPÍRITO SANTO – BRASIL." Veterinária e Zootecnia 29 (March 15, 2022): 1–9. http://dx.doi.org/10.35172/rvz.2022.v29.611.

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As doenças transmitidas por carrapatos são afecções de grande importância na clínica médica de pequenos animais, devido à alta casuística e ampla distribuição vetorial no território brasileiro. Os principais agentes responsáveis pelas infecções em cães são Babesia sp., Ehrlichia canis e Hepatozoon canis. Os animais infectados são assintomáticos ou apresentam sinais clínicos inespecíficos, sendo necessário a utilização de testes diagnósticos para definição do agente etiológico, e diagnóstico seguro. O objetivo do presente estudo foi determinar a ocorrência desses micro-organismos em cães natura
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Araujo, Andreina C., Júlia A. G. Silveira, Sérgio S. Azevedo, et al. "Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil." Pesquisa Veterinária Brasileira 35, no. 5 (2015): 456–61. http://dx.doi.org/10.1590/s0100-736x2015000500012.

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Abstract:This study aimed to report the prevalence of Babesia canis vogeli in dogs and ticks in the urban and rural areas of Petrolina, Pernambuco. Serum and peripheral blood samples of 404 dogs were tested by indirect immunofluorescence assay (IFA) and by blood smears, respectively. The presence of tick infestation was evaluated, and some specimens were submitted to DNA amplification by polymerase chain reaction (PCR). The presence of antibodies anti-B. canis vogeli was determinate in 57.9% (234/404) of dogs. The direct detection of Babesia spp was obtained in 0.5% (2/404) dogs by visualizati
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Carvalho, Tânia Regina Vieira de, Paula Rocha Andrade, and Jonas Moraes Filho. "PESQUISA DE HEMOPARASITOS EM CARRAPATOS COLETADOS EM CÃES RESIDENTES NO MUNICIPIO DE ITU, SÃO PAULO/SP, BRASIL." Brazilian Journal of Global Health 2, no. 8 (2022): 20–22. http://dx.doi.org/10.56242/globalhealth;2022;2;8;20-22.

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Resumo OBJETIVO: Objetivou-se nesse estudo relatar a ocorrência de Ehrlichia canis, Babesia canis vogeli e Rangelia vitalli, em carrapatos coletados em cães residentes no município de Itu_São Paulo/SP, Brasil. MÉTODOS: Foi realizada a extração de DNA de 200 amostras de carrapatos com o “kit” de extração “PureLink Genomic DNA Kit” (Invitrogen®) e realizada a PCR em tempo real para a detecção de Ehrlichia canis, Rangelia vitalli, Babesia canis vogeli. RESULTADOS: Foram testados 200 carrapatos, sendo 1/200 (0,5%) da espécie do gênero Amblyomma aureolatum e 199/200 (99,5%) de Rhipicephalus sanguin
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Dalrymple, B. P., C. M. Dimmock, F. Parrodi, and I. G. Wright. "Babesia bovis, Babesia bigemina, Babesia canis, Babesia microti and Babesia rodhaini: Comparison of ribosomal RNA gene organization." International Journal for Parasitology 22, no. 6 (1992): 851–55. http://dx.doi.org/10.1016/0020-7519(92)90139-c.

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Łyp, P., Ł. Adaszek, B. Furmaga, and S. Winiarczyk. "Identification of new 18S rRNA strains of Babesia canis isolated from dogs with subclinical babesiosis." Polish Journal of Veterinary Sciences 18, no. 3 (2015): 573–77. http://dx.doi.org/10.1515/pjvs-2015-0074.

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Abstract In this study, we used PCR to detect and characterize B. canis from naturally infected dogs in Poland with subclinical babesiosis by amplifying and sequencing a portion of the 18S ribosomal RNA (rRNA) gene. Venous blood samples were collected from ten dogs with subclinical babesiosis. A 559-bp fragment of the B. canis 18S rRNA gene was amplified by PCR. Sequencing of the PCR products led to the identification of a new variant of Babesia canis, differing from the previously detected protozoa genotypes (18S rRNA-A and 18S rRNA-B) with nucleotide substitutions in positions 150 and 151 of
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Skornyakova, O. O. "Identification of the causative agent of canine babesiosis in the Kirov Region." Russian Journal of Parasitology 15, no. 2 (2021): 24–28. http://dx.doi.org/10.31016/1998-8435-2021-15-2-24-28.

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The purpose of the research is identifying a species of the causative agent of canine babesiosis in the Kirov Region.Materials and methods. To determine the parasite species, we microscopically examined smears from the peripheral blood of sick dogs stained by the Romanowsky-Giemsa method and Leucodif. The microscopy was performed with a binocular microscope Micromed-1. For morphometric assessment of the causative agent, we used a digital camera and software for image analysis ToupView.Results and discussion. We found parasites of various shapes and sizes in erythrocytes of the dogs’ blood in a
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RAFIQI, SHAFIYA IMTIAZ, SANJEEV KUMAR, K. K. REENA, et al. "Molecular characterization of Hepatozoon sp. and Babesia sp. isolated from endangered Asiatic lion (Panthera leo persica)." Indian Journal of Animal Sciences 88, no. 6 (2018): 662–66. http://dx.doi.org/10.56093/ijans.v88i6.80880.

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Hemoparasitic infections are frequently encountered in wild carnivores. Although, mostly, the infections are typically asymptomatic, they can be pathogenic under certain circumstances, viz. concurrent disease and stress due to captivity, habitat degradation, adverse climatic conditions or immunosuppression. The present study was undertaken to genotype Babesia sp. and Hepatozoon sp. isolated from lions of Lion Safari, Etawah, Uttar Pradesh, India and establishing phylogenetic relationship based on 18S rRNA sequence with other isolates around the globe. Blood samples of five Asiatic lions, recei
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SCHETTERS, TH P. M., K. MOUBRI, E. PRÉCIGOUT, J. KLEUSKENS, N. C. SCHOLTES, and A. GORENFLOT. "Different Babesia canis isolates, different diseases." Parasitology 115, no. 5 (1997): 485–93. http://dx.doi.org/10.1017/s0031182097001686.

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Using surface immunofluorescence isolate-specific antigens were detected on the membrane of erythrocytes infected with Babesia parasites. In addition, the strains reacted differently with Plasmagel in that the European isolate (B.c. canis) could be purified on Plasmagel effectively, whereas infected erythrocytes of the South-African isolate (B.c. rossi) could not. Experimental infection of dogs with Babesia canis isolates from geographically different areas revealed different pathology. The European isolate obtained from France exhibited transient parasitaemia, usually below 1%, associated wit
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Baneth, G., M. J. Kenny, S. Tasker, et al. "Infection with a Proposed New Subspecies of Babesia canis, Babesia canis subsp. presentii, in Domestic Cats." Journal of Clinical Microbiology 42, no. 1 (2004): 99–105. http://dx.doi.org/10.1128/jcm.42.1.99-105.2004.

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Bajer, Anna, Maciej Kowalec, Viktoriya A. Levytska, et al. "Tick-Borne Pathogens, Babesia spp. and Borrelia burgdorferi s.l., in Sled and Companion Dogs from Central and North-Eastern Europe." Pathogens 11, no. 5 (2022): 499. http://dx.doi.org/10.3390/pathogens11050499.

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Ticks are important vectors of numerous pathogens of medical and veterinary significance. The aim of the current study was to determine the prevalence of Babesia spp. and Borrelia burgdorferi s.l. in sled and pet dogs from Central and North-Eastern Europe. Neither Babesia spp. nor Borrelia burgdorferi s.l. infections were detected in sled dogs from seven countries (Poland, Lithuania, Latvia, Estonia, Belarus, Russia and Finland). The DNA of Babesia spp. was detected in 100% of symptomatic and 5.4% of asymptomatic pet dogs from Poland. Similarly, the DNA of Babesia spp. was identified in 82% of
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Gonzales Barbosa, Liliana, and Luis Alfonso Lesmes. "Determinación de Babesia canis y Ehrlichia canis, en hemolinfa de garrapatas teleoginas de Riphicephalus sanguineus." Revista Sistemas de Producción Agroecológicos 4, no. 1 (2013): 66–77. http://dx.doi.org/10.22579/22484817.611.

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El presente estudio se llevó a cabo en el laboratorio de microbiología de la Universidad de los Llanos Orientales, ubicada a 4 km de la ciudad de Villavicencio (Meta), con una temperatura promedio de 28°C, humedad relativa del 80% y una altura de 440 msnm. El objetivo fue determinar la presencia de Babesia canis y Ehrlichia canis, por medio del extendido y coloración de Giemsa en la hemolinfa de garrapatas teleoginas de Riphicephalus sanguineus (RS), mediante capturadas en perros de la ciudad de Villavicencio, utilizando la técnica de identificación propuesta por López (1980); con el fin de co
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Tadesse, Hana, Marika Grillini, Giulia Simonato, et al. "Epidemiological Survey on Tick-Borne Pathogens with Zoonotic Potential in Dog Populations of Southern Ethiopia." Tropical Medicine and Infectious Disease 8, no. 2 (2023): 102. http://dx.doi.org/10.3390/tropicalmed8020102.

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Dogs are known to host several tick-borne pathogens with zoonotic potential; however, scant information is available on the epidemiology of these pathogens in low-income tropical countries and in particular in sub-Saharan Africa. With the aim of investigating a wide range of tick-borne pathogens (i.e., Rickettsia spp., Anaplasma spp., Erhlichia spp., Borrelia spp., Hepatozoon spp. and Babesia spp.), 273 blood samples were collected from dogs in selected districts of Ethiopia and analyzed by real-time and/or end-point PCR. The results of the study showed that Hepatozoon canis was the most preva
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Scott, John D., and Risa R. Pesapane. "Detection of Anaplasma phagocytophilum, Babesia odocoilei, Babesia sp., Borrelia burgdorferi Sensu Lato, and Hepatozoon canis in Ixodes scapularis Ticks Collected in Eastern Canada." Pathogens 10, no. 10 (2021): 1265. http://dx.doi.org/10.3390/pathogens10101265.

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Tick-borne pathogens cause infectious diseases that inflict much societal and financial hardship worldwide. Blacklegged ticks, Ixodes scapularis, are primary vectors of several epizootic and zoonotic pathogens. The aim was to find varius pathogens of I. scapularis and to determine their prevalence. In Ontario and Quebec, 113 I. scapularis ticks were collected from songbirds, mammals, including humans, and by flagging. PCR and DNA sequencing detected five different microorganisms: Anaplasma phagocytophilum, 1 (0.9%); Babesia odocoilei, 17 (15.3%); Babesia microti-like sp., 1 (0.9%); Borrelia bu
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Al Izzi, Salah, Donald S. Martin, Roxanne Y. Y. Chan, and Christian M. Leutenegger. "Babesia canis vogeli,Ehrlichia canis, andAnaplasma platysinfection in a dog." Veterinary Clinical Pathology 42, no. 4 (2013): 471–75. http://dx.doi.org/10.1111/vcp.12090.

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Berzina, Inese, Valentina Capligina, Viesturs Baumanis, Renate Ranka, Dina Cirule, and Ilze Matise. "Autochthonous canine babesiosis caused by Babesia canis canis in Latvia." Veterinary Parasitology 196, no. 3-4 (2013): 515–18. http://dx.doi.org/10.1016/j.vetpar.2013.03.015.

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Silaghi, Cornelia, Lisa Weis, and Kurt Pfister. "Dermacentor reticulatus and Babesia canis in Bavaria (Germany)—A Georeferenced Field Study with Digital Habitat Characterization." Pathogens 9, no. 7 (2020): 541. http://dx.doi.org/10.3390/pathogens9070541.

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The hard tick Dermacentor reticulatus transmits Babesia canis, the causative agent of canine babesiosis. Both the occurrence and local distribution of D. reticulatus as well as infection rates of questing ticks with B. canis are thus far poorly known in Bavaria, Germany. The objectives of this study were to conduct (1) a georeferenced field study on the occurrence of D. reticulatus with digital habitat characterization and (2) a PCR analysis of D. reticulatus collected in Bavaria for infection with B. canis. Dermacentor reticulatus were collected by flagging at 60 sites specifically selected a
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Gulyukin, E. "DEPENDENCE OF THE EFFECTIVENESS OF LIGHT IMMERSION MICROSCOPY ON THE TIME OF INFECTING WITH BABESIASIS (BABESIA CANIS)." THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL, no. 23 (April 18, 2022): 156–60. http://dx.doi.org/10.31016/978-5-6046256-9-9.2022.23.156-160.

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As a result of the studies, it was determined that the identification of Babesia canis by light immersion microscopy is not effective on the day the ixodid tick is detected on the animal; however, when clinical signs appear, this method makes it possible to detect Babesia canis in a blood smear of the animal with maximum accuracy. It was also found during the study that the optimal time for contacting veterinary clinics to diagnose babesiosis (piroplasmosis) by light immersion microscopy and receiving effective treatment is 3–5 days from the time the tick was detected. But, it is worth remembe
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Řeháčková, K., M. Haláková, B. Víchová, and A. Kočišová. "Epizootiological Study of the Occurrence of Canine Babesiosisin Southwestern Slovakia." Folia Veterinaria 60, no. 3 (2016): 39–42. http://dx.doi.org/10.1515/fv-2016-0027.

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Abstract This epizootiological study was carried out to investigate the occurrence of canine babesiosis in southwestern Slovakia. The study focused on the proportion of the species of ticks serving as babesia vectors of babesiosis in the ticks collected from selected locations close to Komárno, in southwestern Slovakia. Additionally, observations were made on the health and overall clinical signs in dogs suspected of having babesiosis. In 2014 we collected ticks from vegetation by the drag cloth (flagging) method and also directly from dogs. A totally of 622 ticks were collected: 491 Dermacent
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Do, Thom, Pornkamol Phoosangwalthong, Ketsarin Kamyingkird, Chanya Kengradomkij, Wissanuwat Chimnoi, and Tawin Inpankaew. "Molecular Detection of Tick-Borne Pathogens in Stray Dogs and Rhipicephalus sanguineus sensu lato Ticks from Bangkok, Thailand." Pathogens 10, no. 5 (2021): 561. http://dx.doi.org/10.3390/pathogens10050561.

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Canine tick-borne pathogens (CTBPs) such as Babesia vogeli, Ehrlichia canis, Anaplasma platys, Hepatozoon canis, and Mycoplasma haemocanis are important pathogens in dogs worldwide. Rhipicephalus sanguineus sensu lato, the main vector of several CTBPs, is the most common tick species found on dogs in Thailand. The present study identified CTBPs in dogs and ticks infested dogs. Samples (360 dog blood samples and 85 individual ticks) were collected from stray dogs residing in 37 temples from 24 districts in Bangkok and screened for CTBPs using molecular techniques. The most common CTBP found inf
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Puchalski, A., M. Dec, S. Winiarczyk, and L. Adaszek. "Analysis of the culture-derived soluble Babesia canis canis antigens derived from the Polish strains of the parasites." Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere 40, no. 06 (2012): 399–403. http://dx.doi.org/10.1055/s-0038-1623671.

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Summary Objective, material and methods: The aim of this study was to analyse the protein fractions of the soluble parasitic antigen (SPA) from in vitro cultures of the native Polish strains of Babesia canis canis and to determine their immunogenicity through Western blotting using the sera of dogs vaccinated with this antigen. Results: Polyacrylamide gel electrophoresis revealed 21 protein fractions with molecular weights from 12 to 205 kDa. The most intense reaction in Western blotting was observed between the serum antibodies of the SPA-vaccinated dogs and the fraction with the molecular we
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Reichert, Nika S., Daniela Mathieu, Christopher J. Katz, and Kent A. Hatch. "Exposure of American Black Bears (Ursus americanus) to Ticks, Tick-Borne Diseases, and Intestinal Parasites in Wisconsin." Diversity 16, no. 9 (2024): 537. http://dx.doi.org/10.3390/d16090537.

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We surveyed 159 American black bears (Ursus americanus) over a period of three years for the occurrence of ticks, tick-borne diseases, and intestinal parasites in Wisconsin. We collected blood from the bears to test for the presence of antibodies to Borrelia burgdorferi (Lyme disease), Rickettsia rickettsii (Rocky Mountain spotted fever (RMSF)), Babesia, Ehrlichia, Ehrlichia canis, Brucella canis, and Anaplasma phagocytophilum. We also examined scat samples for intestinal parasites. We commonly found the tick Dermacentor variabilis, but also present the first report of Rhipicephalus sanguineus
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Zygner, W., O. Gójska-Zygner, and H. Wędrychowicz. "Changes in the SUSPPUP ratio and fractional excretion of strong monovalent electrolytes in hospitalized dogs with canine babesiosis." Polish Journal of Veterinary Sciences 15, no. 4 (2012): 791–92. http://dx.doi.org/10.2478/v10181-012-0119-y.

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Abstract In this study an increased SUSPPUP ratio and fractional excretion of potassium in dogs infected with Babesia canis suggested mineralocorticoid excess in canine babesiosis. A significant increase in strong monovalent electrolyte fractional excretions in azotaemic dogs infected with B. canis probably resulted from acute tubular necrosis.
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