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Journal articles on the topic 'Bacillus (Bacteria) – Identification'

Author: Grafiati

Published: 4 June 2021

Last updated: 13 February 2022

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1

Sepriana, Citra, and Eti Sumiati. "Identifikasi Dan Uji Daya Hambat Isolat Bakteri Endofit Bunga Tanaman Cengkeh (Syzygium aromaticum L.) Terhadap Bakteri Patogen." Jurnal Penelitian Pendidikan IPA 6, no. 1 (2020): 101. http://dx.doi.org/10.29303/jppipa.v6i1.340.

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This research was conducted to find out the capabilities of endophytic bacteria isolated from flowers of the clove plants in inhibiting the growth of bacteria Streptococcus mutans, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli and to identify endophytic bacteria that potensial to produce an antibacterial. Stages of this research include the isolation of endophytic bacteria from flowers of the clove plants, antibacterial test, and molecular identification based on 16S rRNA. Isolates of endophytic bacterial of clove plants flower produce 5 isolates, 4 isolate inhibited the ba

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Zebua, Abdi Hikmat Petra, Nursyirwani Nursyirwani, and Feliatra Feliatra. "MOLECULAR IDENTIFICATION OF PROTEOLITIC BACTERIA FROM MANGROVE SEDIMENT IN DUMAI MARINE STATION." Asian Journal of Aquatic Sciences 3, no. 2 (2020): 179–88. http://dx.doi.org/10.31258/ajoas.3.2.179-188.

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Proteolytic bacteria have an important role in the degradation of complex compounds to a simple compounds by the enzyme proteases. Sediment in the mangrove ecosystem of Dumai Marine Station may contain the bacteria. This research aims to identify the proteolytic bacteria of mangrove sediments molecularly and to examine the antagonism of pathogenic bacteria. The method used in this research was a survey. The results obtained 10 bacterial isolates (AZ1, AZ2, AZ3, AZ4, AZ6, AZ10, AZ11, AZ15, AZ18, and AZ20). Identification using 16S rRNA analysis revealed that 3 isolates showed different results,

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Watanabe, Katsuji, and Koichi Hayano. "Distribution and identification of proteolytic Bacillus spp. in paddy field soil under rice cultivation." Canadian Journal of Microbiology 39, no. 7 (1993): 674–80. http://dx.doi.org/10.1139/m93-097.

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Proteolytic bacteria in paddy field soils under rice cultivation were characterized and enumerated using azocoll agar plates. Bacillus spp. were the proteolytic bacteria that were most frequently present, comprising 59% of the isolates. They were always the numerically dominant proteolytic bacteria isolated from three kinds of fertilizer treatments (yearly application of rice-straw compost and chemical fertilizer, yearly application of chemical fertilizer, and no fertilizer application) and at three different stages of rice development (vegetative growth stage, maximal tillering stage, and har

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Hasan, R., M. N. H. Rony, M. S. A. Sarker, M. Z. Tareq, and R. Ahmed. "Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh." Journal of Bioscience and Agriculture Research 26, no. 02 (2020): 2211–16. http://dx.doi.org/10.18801/jbar.260220.270.

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Bacteria are essential elements of natural environments. As bacteria are the key critical components of food webs and nutrient cycles, they contribute to ecosystem functioning via mutualists and pathogens for larger species. The present study has provided substantial grounds to confirm that microbial communities present in natural environments are much more diverse. Here, we tend to study a singular environment Lawachara National Park. Total 125 bacterial strains were isolated using serial dilution method. Thirteen unique colonies were selected, cultured and characterized by gram staining and

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Ngalimat, Mohamad Syazwan, Raja Noor Zaliha Raja Abd. Rahman, Mohd Termizi Yusof, Amir Syahir, and Suriana Sabri. "Characterisation of bacteria isolated from the stingless bee, Heterotrigona itama, honey, bee bread and propolis." PeerJ 7 (August 22, 2019): e7478. http://dx.doi.org/10.7717/peerj.7478.

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Bacteria are present in stingless bee nest products. However, detailed information on their characteristics is scarce. Thus, this study aims to investigate the characteristics of bacterial species isolated from Malaysian stingless bee, Heterotrigona itama, nest products. Honey, bee bread and propolis were collected aseptically from four geographical localities of Malaysia. Total plate count (TPC), bacterial identification, phenotypic profile and enzymatic and antibacterial activities were studied. The results indicated that the number of TPC varies from one location to another. A total of 41 d

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Jung, Ryan, Minzae Kim, Bhoomi Bhatt, Jong Choi, and Jung Roh. "Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis." International Journal of Environmental Research and Public Health 16, no. 6 (2019): 912. http://dx.doi.org/10.3390/ijerph16060912.

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Hazardous organisms may thrive on surfaces that are often exposed to human contact, including children’s library books. In this study, swab samples were taken from 42 children’s books collected from four public libraries in Texas and California. Samples were then cultivated in brain–heart infusion (BHI) medium and then in Luria broth (LB) medium containing either ampicillin or kanamycin. All 42 samples (100%) were positive for bacterial growth in normal BHI medium. Furthermore, 35 samples (83.3%) and 20 samples (47.6%) in total were positive in LB medium containing ampicillin or kanamycin, res

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Sijabat, Octanina Sari, Marheni Marheni, and Darma Bakti. "THE IDENTIFICATION OF BACTERIAL SYMBIONT’S OF THE LARVAE ORYCTES RHINOCEROS L. AND THE ROLE OF THE BACTERIA IN COMPOSTING PROCESS." Journal of Community Research and Service 1, no. 2 (2018): 43. http://dx.doi.org/10.24114/jcrs.v1i2.9334.

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AbstractOryctes rhinoceros L. has symbioses with micro organisms in their hind guts which further break down plant material consumed by beetle. The aim of this research is to determine the identification of the existence of the bacterial species in the hind gut larvae of the symbiotic bacteria using biochemical test and analysis based on 16S rRNA. The result of this research indicate that there were two different bacterials: Bacillus siamensis and Bacillus stratosphericus found. The bacteria was used for starting the composting and more specifically, the Bacillus siamensis can speed up compost

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Haakensen, Monique, and Barry Ziola. "Identification of novel horA-harbouring bacteria capable of spoiling beer." Canadian Journal of Microbiology 54, no. 4 (2008): 321–25. http://dx.doi.org/10.1139/w08-007.

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An ATP-binding cassette (ABC) multi-drug resistance (MDR) gene was found in 4 Gram-positive bacterial isolates of environmental origin and found capable of spoiling beer. The bacteria isolated were Bacillus cereus , Bacillus licheniformis , Paenibacillus humicus , and Staphylococcus epidermidis ; all of which were previously unappreciated as beer-spoilage bacteria. The MDR gene found in these bacteria has less than 37% similarity to known ABC MDR proteins described for Bacillus and Staphylococcus , and this is the first finding of an ABC MDR gene in the genus Paenibacillus . The sequenced regi

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Ikerismawati, Senja. "BIOREMEDIASI Pb OLEH BAKTERI INDIGEN LIMBAH CAIR AGAR." Jurnal Biosilampari : Jurnal Biologi 1, no. 2 (2019): 51–58. http://dx.doi.org/10.31540/biosilampari.v1i2.288.

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Bacteria isolated from ad environment contaminated with heavy metals are very potential as heavy metal bioremediation agents called indigenous bacteria. The purpose of this study was to isolate and identify indigenic bacteria that have the potential as Pb bioremediation agents in agar liquid waste and to analyze the ability of indigenic bacteria in reducing Pb. The research design used Completely Randomized Design (RAL) two factorial with variation of bacteria and eight days of treatment. Data were analyzed using two-way ANOVA with Duncan's advanced test. The results showed that there were eig

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Saha, Mihir Lal, Khondokar Nowshin Islam, Taslima Akter, Iffat Ara Rahman, Tahmina Islam, and Tahsin Khan. "Isolation and identification of amylolytic bacteria from garbage and garden soil." Bangladesh Journal of Botany 48, no. 3 (2019): 537–45. http://dx.doi.org/10.3329/bjb.v48i3.47915.

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An analysis for the abundance and diversity of amylolytic bacteria of two different soil types viz. garbage and garden soil was carried out. pH of the garbage and garden soil samples ranged between 7.73 and 9.84, 6.88 and 7.93, respectively. Average bacterial load on both NA and PYG agar media was found to be higher in garbage than garden soils. Bacterial load of garbage soil samples ranged from 2.08 × 108 to 3.79 ×108 cfu/g and 1.45 × 108 to 2.74 × 108 cfu/g on NA and PYG agar, respectively. On the other hand, bacterial load of the garden soil samples ranged from 3.3×106 to 9.7 ×106 cfu/g on

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Suldina, Е. V., N. А. Feoktistova, and I. I. Bogdanov. "ISOLATION OF NEW STRAINS OF BACILLUS MEGATERIUM BACTERIA AND STUDY OF THEIR BIOLOGICAL PROPERTIES." Vestnik of Ulyanovsk state agricultural academy, no. 3(50) (September 8, 2020): 60–67. http://dx.doi.org/10.18286/1816-4501-2020-3-60-67.

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Bacillus megaterium is a gram - positive rod-shaped endospore-forming bacteria. They are used as an effective soil modifier, having the ability to disperse phosphorus. In turn, phosphorus plays an important role in several physiological and biochemical processes of plants, such as photosynthesis, the conversion of sugar to starch, and the transfer of genetic traits. Based on the current state of research in the development of biofertilizers, it can be argued that the biocomposition of Bacillus megaterium bacteria will lead to an increase in the availability of a number of macronutrients for pl

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Nurfitri, Nurfitri, Fiddy Semba Prasetiya, Indah Riyantini, Yuniar Mulyani, Sulastri Arsad, and Mochamad Untung Kurnia Agung. "Study of Symbiont Bacteria of Acropora digitifera Coral From Ciletuh Bay, Sukabumi by Using Culture and Molecular Approach." Omni-Akuatika 15, no. 2 (2019): 106. http://dx.doi.org/10.20884/1.oa.2019.15.2.748.

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Bacteria are one of the prokaryotic microorganisms that are symbiotic with coral reefs. These microorganisms help corals in secreting mucus layers which are used as a place to live for bacteria and control the presence of pathogenic bacteria in corals. The purpose of this study was to determine the diversity of bacterial communities associated with Acropora digitifera corals in Ciletuh bay, West Java, by culture approach. Sampling was carried out at a depth of 4 meters. Morphology-based identification and molecular approach based on sequence of the small ribosomal unit (16S) rRNA gene were use

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Ghazouani, Abdullah, Khaled El-Gayar, and Emad Abada. "Molecular characterization of gene encoding halo tolerant amylase of Bacillus paralicheniformis isolated from Jazan region." Modern Journal of Microbial Biology 2 (November 27, 2020): 1. http://dx.doi.org/10.25259/mjmb_2_2020.

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Objectives: This study aims to characterize the gene encoding halo tolerant amylase of bacteria isolated from Jazan region. Materials and Methods: Soil samples were collected from several area of Jazan region, KSA. The samples were serially diluted and plateted on starch agar plates. The amylase producing bacteria were detected by iodine test. To determine the halophilic amylase producing bacteria, several colonies were tested for their ability to grow at higher concentrations of NaCl ranging from 7 to 16%. The bacteria was identified by 16S rRNA and the full length amylase gene was fully iden

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Resti, Zurai, Trimurti Habazar, Deddi Prima Putra, and Nasrun Nasrun. "SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH." Jurnal Hama dan Penyakit Tumbuhan Tropika 13, no. 2 (2013): 167–78. http://dx.doi.org/10.23960/j.hptt.213167-178.

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Screening and identification of endophytic bacteria to control bacterial leaf blight disease on Shallot. The experiment was conducted in Laboratory and Green House, from January to June 2012. Laboratory experiment consisted of three steps: (1) isolation of endophytic bacteria from healthy onion roots, (2)In planta /screening of endophytic isolates capable of reducing bacterial leaf blight disease, and (3) molecular identification of potential endophytic isolates. Treatments of in planta test were arranged in Completely Randomized Design. Collected isolates were tested for their capability in c

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Puspitasari, Dhewanti, Hendro Pramono, and Oedjijono Oedjijono. "IDENTIFIKASI BAKTERI PENGOKSIDASI BESI DAN SULFUR BERDASARKAN GEN 16S rRNA DARI LAHAN TAMBANG TIMAH DI BELITUNG." Scripta Biologica 1, no. 1 (2014): 10. http://dx.doi.org/10.20884/1.sb.2014.1.1.12.

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Heavy metals contamination disturb balance and diversity of microorganism in soil. Microorganisms which can able to survive in those conditions are bacteria capable of oxidizing heavy metals. Identification based on 16S rRNA was used to determine characteristics and phylogenetic relationship of bacteria which can oxidize iron and sulphur in tin mining areas. The aim of this research was able to determine the bacterias characteristics isolated from tin mining areas and determine the phylogenetic relation of iron-sulphur oxidizing bacteria on tin mining soil in Belitung based on 16S rRNA sequenc

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Kurniawan, Ardiansyah, Suci Puspita Sari, Euis Asriani, Andi Kurniawan, Abu Bakar Sambah, and Asep Awaludin Prihanto. "Molecular Identification of Cellulolytic Bacteria From Mangrove Sediment at Tin Minning Region In West Bangka." International Journal of Applied Biology 3, no. 1 (2019): 7. http://dx.doi.org/10.20956/ijab.v3i1.5848.

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Cellulose as an abundant source of glucose in Indonesia requires acceleration of decomposition utilizing cellulolytic bacteria. Cellulolytic bacteria can be obtained from the isolation of mangrove organic matter, such as sediments. Muntok Sub-district is one of the regions with the most tin mining in West Bangka Regency also has mangroves in the coastal area. Exploration of cellulolytic bacteria in mangroves with different environmental characteristics encourages researchers to find new bacterial strains that produce cellulase enzymes with new properties. Thirteen isolates were successfully is

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De Clerck, E., T. Vanhoutte, T. Hebb, J. Geerinck, J. Devos, and P. De Vos. "Isolation, Characterization, and Identification of Bacterial Contaminants in Semifinal Gelatin Extracts." Applied and Environmental Microbiology 70, no. 6 (2004): 3664–72. http://dx.doi.org/10.1128/aem.70.6.3664-3672.2004.

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ABSTRACT Bacterial contamination of gelatin is of great concern. Indeed, this animal colloid has many industrial applications, mainly in food and pharmaceutical products. In a previous study (E. De Clerck and P. De Vos, Syst. Appl. Microbiol. 25:611-618), contamination of a gelatin production process with a variety of gram-positive and gram-negative bacteria was demonstrated. In this study, bacterial contamination of semifinal gelatin extracts from several production plants was examined. Since these extracts are subjected to harsh conditions during production and a final ultrahigh-temperature

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Luang-In, Vijitra, Manatchanok Yotchaisarn, Worachot Saengha, Piyachat Udomwong, Sirirat Deeseenthum, and Kedsukon Maneewan. "Isolation and Identification of Amylase-producing Bacteria from Soil in Nasinuan Community Forest, Maha Sarakham, Thailand." Biomedical & Pharmacology Journal 12, no. 3 (2019): 1061–68. http://dx.doi.org/10.13005/bpj/1735.

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This study aimed to isolate and identify bacteria that can produce amylase enzyme from the unexplored Nasinuan Forest, Kantarawichai District, Mahasarakham Province, Thailand. Thirteen bacterial isolates with amylase-producing capacity on 1% starch agar were identified using 16S rRNA sequencing. Twelve bacteria were gram-positive, rod shaped and identified as Bacillus spp. and one bacterium with gram-negative and rod shaped character was Enterobacter cloacae. Their closest relatives were found in India, China, Korea, Indonesia, Argentina, Italy, Israel, USA, Argentina and South Africa. These b

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Pașca, Claudia, Liviu Alexandru Mărghitaș, Ioana Adriana Matei, et al. "Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation." Applied Sciences 11, no. 13 (2021): 5816. http://dx.doi.org/10.3390/app11135816.

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This study aimed to characterize raw honeys from different geographical origins in Romania, in respect of chemical composition, microbiological examination and evaluate their probiotic potential. The physico-chemical determinations were performed in APHIS-DIA Laboratory, Cluj-Napoca, Romania, using standard validated methods. Bacterial identification was performed for each sample and each colony type using Vitek® 2 Compact 15 system and PCR amplification using 16S rDNA bacterial universal primers (27F, 1492R), species being confirm by sequences analysis. In five raw honey samples, we have iden

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Gunaedi, Tri, Sebastian Margino, Langkah Sembiring, and Rarastoeti Pratiwi. "IDENTIFIKASI DAN KLASIFIKASI BAKTERI AMILOLITIK ISOLAT TG12, TG19, DAN TG31 PENYEBAB KEMASAMAN PADA TEPUNG SAGU BASAH BERDASARKAN ANALISIS GEN 16SrDNA." Berkala Penelitian Hayati 15, no. 1 (2009): 25–30. http://dx.doi.org/10.23869/bphjbr.15.1.20096.

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16SrDNA gene were known essentialy for procaryotic life involved bacteria. The gene very concerved such as usefull for bacterial identification and classification in phylogeny tree constructed. The object of this research were identified and cllassified amylolitic bacteria TG12, TG19 and TG31 isolates, causers sourness on raw starch sago by 16SrDNA gene sequences analysis approach. The native isolates from raw starch sago under traditionality processing arround Jayapura and selected depend on activity amylolitic and organic acid productivity. Before DNA genom extraction, isolates were throught

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Wong, F. H., L. H. Lim, and S. S. Teo. "Identification of bacteria species in aquaponics system using RapID system and 16S rRNA sequencing." Research Journal of Biotechnology 16, no. 7 (2021): 1–14. http://dx.doi.org/10.25303/167rjbt121.

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Aquaponics is an integrated production system of aquaculture and hydroponics. Beneficial bacteria convert the ammonia and solid wastes produced by fish in the aquaculture component into essential nutrients for plant growth in hydroponics. The objective of the present study was to identify the bacteria community in the aquaponics system. This study was set by using barb fish and basils as organisms of cultivation. The fish tank (aquaculture) water was pumped into the treatment tank containing bio-ring functions as nitrifying bacteria by converting ammonia to nitrates. The nutrient-rich water fr

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Enaigbe, A. A., O. S. Imade, O. A. Akpoka, E. Ufuah, O. E. Izevbuwa, and S. O. Okhuebor. "Characterization of Autochthonous Bacterial Isolates with The Potentiality to Degrade Spent Engine Oil from Contaminated Soil Area Enriched with Glycine max." Journal of Applied Sciences and Environmental Management 24, no. 8 (2020): 1463–66. http://dx.doi.org/10.4314/jasem.v24i8.23.

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This study was conducted to identify and characterize bacteria capable of degrading spent oil contaminated soil. The physicochemical parameters of used engine oil were analyzed according to existing standards, while the total heterotrophic plate counts (HBC) and hydrocarbon utilizing bacteria counts were ascertained with the pour plate methods using nutrient agar and minimal salt agar (MSA) media respectively. The results indicated a mean total HBC ranging from 2.86 ± 0.08 to 5.76 log10 CFU/g and mean hydrocarbon utilizing bacterial counts from 1.32 ± 0.09 to 3.82 ± 0.25 log10 CFU/g, with samp

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Weerasinghe, Weerasinghe Mudiyanselage Lakshika Iroshani, Dampe Acharige Tharindu Madusanka, and Pathmalal Marakkale Manage. "Isolation and Identification of Cellulase Producing and Sugar Fermenting Bacteria for Second-Generation Bioethanol Production." International Journal of Renewable Energy Development 10, no. 4 (2021): 699–711. http://dx.doi.org/10.14710/ijred.2021.35527.

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Over the last decades, the negative impacts of fossil fuel on the environment and increasing demand for energy due to the unavoidable depletion of fossil fuels, has transformed the world’s interests towards alternative fuels. In particular, bioethanol production from cellulosic biomass for the transportation sector has been incrementing since the last decade. The bacterial pathway for bioethanol production is a relatively novel concept and the present study focused on the isolation of potential “cellulase-producing” bacteria from cow dung, compost soil, and termite gut and isolating sugar ferm

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Ainul, Asri, M. Hasbi, and Eko Purwanto. "Isolation and Identification of Biosurfactant Producing Bacteria From Workshop Wastewater." Ilmu Perairan (Aquatic Science) 9, no. 1 (2021): 31. http://dx.doi.org/10.31258/jipas.9.1.p.31-37.

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Automotive workshop activities produce oil ills that may pollute waters around the workshop area. The oil-polluted water may inhabit biosurfactant producing bacteria that are able to degrade the oil. A study aimed to isolate and identify the bacteria has been conducted from July to September 2020. The bacteria samples were sampled from workshop wastewater at Kubang Raya street KM 2,5 Pekanbaru, Riau Province and sampling were conducted three times. The bacteria were isolated using TSB (Tryptone Soy Broth) and TSA (Tryptone Soy Agar) media and were identified by using biochemical methods. Resul

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Feoktistova, N. A. "Development and testing of bacteriological diagram of bacillus cereus bacteria identification." Vestnik of Ulyanovsk state agricultural academy, no. 4(48) (December 20, 2019): 144–52. http://dx.doi.org/10.18286/1816-4501-2019-4-144-152.

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Krishna, Lal. "THE IDENTIFICATION, PRODUCTION AND CHARACTERIZATION OF NATTOKINASE, BACTERIOCIN FROM BACTERIAL SPECIES." Bacterial Empire 2, no. 4 (2019): 91. http://dx.doi.org/10.36547/be.2019.2.4.91-95.

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The study was aimed at identification, production and characterization of nattokinase, bacteriocin from bacterial species. Nattokinase and bacteriocins finds a wide range of applications in Pharmaceutical industry, health care and medicine. Nattokinase is a highly active fibrinolytic enzyme secreted by Bacillus subtilis and bacteriocins are proteinaceous toxins produced by Lactobacillus to inhibit the growth of closely related bacterial strains. Bacillus subtilis and Lactobacillus isolates shown positive results to microscopic, biochemical analysis. The nattokinase and bacteriocins were produc

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Masrukhin, Masrukhin, Ade Lia Putri, Tri Ratna Sulistiyani, et al. "Antifungal Activity of Bacterial Isolates from Straw Mushroom Cultivation Medium against Phytopathogenic Fungi." Journal of Tropical Biodiversity and Biotechnology 6, no. 1 (2021): 59235. http://dx.doi.org/10.22146/jtbb.59235.

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Several bacteria were isolated from straw mushroom (Volvariella volvacea) cultivation medium. There are three potential isolates previously characterized and has growth inhibition effect against V. volvacea. This screening result lead to the further study about the inhibition activity against phytopathogenic fungi. The aim of this research is to investigate the antifungal activity of three bacterial isolates against three phytopathogenic fungi and identification of the bacteria. The method used in this study are antifungal assay using co-culture method and disk difussion assay using the filtra

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Jannah, Akyunul, Aulanni`am Aulanni`am, Tri Ardyati, and Suharjono Suharjono. "Isolation, Cellulase Activity Test and Molecular Identification of Selected Cellulolytic Bacteria Indigenous Rice Bran." Indonesian Journal of Chemistry 18, no. 3 (2018): 514. http://dx.doi.org/10.22146/ijc.26783.

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Rice bran is the waste product of rice milling which is abundant in Indonesia, it can be used as a raw material for the manufacture of bioethanol by fermentation. Before being fermented, rice bran must be hydrolyzed into glucose by biomass degrading. This study was aimed to isolate indigenous cellulolytic bacteria from rice bran as producer of cellulolytic enzymes and resulted in 22 bacterial isolates that demonstrated cellulolytic activity being identified. Among them, BE 8 and BE 14 isolates showed the highest endoglucanase activity at 2.16 and 1.31 U/mL respectively. Identification of the 1

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Andriani, Yuli, Emma Rochima, Ratu Safitri, and Sri Rejeki Rahayuningsih. "Characterization of Bacillus megaterium and Bacillus mycoides Bacteria as Probiotic Bacteria in Fish and Shrimp Feed." KnE Life Sciences 2, no. 6 (2017): 127. http://dx.doi.org/10.18502/kls.v2i6.1029.

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This study was aimedto identify probiotic characteristics and to test the cellulolytic ability of Bacillus megaterium and Bacillus mycoides bacteria for probiotic microbe candidates in fish and shrimp feed. The description of the cellulolytic and amylolytic abilities of these bacteriawas obtained by non-experimental method and descriptive analysis. Probiotic characteristic identification includes growth curve was obtained through total plate count method, cellular and colony morphology, and cellulase and amylase enzyme activity test using DNS method. Results indicated that the maximum growth o

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Abdullah, Nur Syazrin Amalina, Shareena Fairuz Abdul Manaf, Nur Shahidah Ab Aziz, Fazlena Hamzah, and Norazlina Idris. "Characterization and Identification of Isolated Microorganism from Agricultural Soil for Degradation of Cellulose Based Waste." Advanced Materials Research 1113 (July 2015): 230–35. http://dx.doi.org/10.4028/www.scientific.net/amr.1113.230.

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Paper wastes accumulating in the environment are posing an ever increasing ecological threat. This study aimed to isolate soil bacteria closely associated with the bacteria degrading waste paper. A total of six soil samples were collected from dumped soil hostel garden at Universiti Teknologi MARA, Shah Alam. Bacillus, Aspergillus and Micrococcus species were found to be the most widely distributed on the entire sample taken. Six cultures were screened for the ability to degrade cellulose. Bacillus was identified as only bacteria isolated that can degrade paper at highest production of glucose

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Priadie, Bambang. "TEKNIK BIOREMEDIASI SEBAGAI ALTERNATIF DALAM UPAYA PENGENDALIAN PENCEMARAN AIR." Jurnal Ilmu Lingkungan 10, no. 1 (2012): 38. http://dx.doi.org/10.14710/jil.10.1.38-48.

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ABSTRAK Walaupun telah diberlakukan berbagai macam kebijakan dan peraturan terkait dengan pengendalian pencemaran air, namun penurunan kualitas badan air masih terus berlangsung. Hal ini disebabkan karena lemahnya pengawasan dan penegakan hukum maupun teknologi pengendalian pencemaran air yang berbasis pembubuhan bahan kimia masih belum bisa memenuhi kriteria yang diberlakukan. Tulisan ini menguraikan proses bioremediasi sebagai alternatif dalam upaya pengendalian pencemaran air, meliputi: isolasi, pengujian degradasi zat pencemar, dan perbanyakan bakteri. Hasil isolasi dan identifikasi yang b

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Rennie, R. J. "The API 20B microtube system to aid in the identification of N2-fixing Bacillaceae." Canadian Journal of Microbiology 33, no. 6 (1987): 504–9. http://dx.doi.org/10.1139/m87-084.

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N2-fixing bacterial isolates from soil have been identified successfully using the API 20E and 50E microtubes in conjunction with a computer-assisted biochemical profile search. The 20E and 50E result in few positive tests with N2-fixing Bacillaceae. Recently, a new battery of 20 microtube tests, the 20B, has been introduced for identifying aerobic, heterotrophic bacteria isolated from natural environments. This system was compared with the 20E and evaluated for N2-fixing bacillus species from soil. It was then used in conjunction with guanine-plus-cytosine analysis to identify the C-11-25 (5D

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Rahman, Md Tanvir, Md Shafiqul Islam, and Mahmudul Hasan. "Isolation and Identification of Bacterial Agents Causing Clinical Mastitis in Cattle in Mymensingh and Their Antibiogram Profile." Microbes and Health 2, no. 1 (2014): 19–21. http://dx.doi.org/10.3329/mh.v2i1.17258.

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Mastitis is a serious problem for the dairy cattle in many countries of the world including Bangladesh. Among the microbial etiology bacteria plays a major role in the onset of the clinical form of the disease. Many of these bacteria are resistant to one or more antibiotics thus make the mastitis cases difficult to cure. In the present study Staphylococcus spp., Streptococcus spp., Bacillus spp. and E. coli were identified as the dominant bacterial species causing clinical mastitis in cattle in Mymensingh. Antibiogram study revealed chloramphenicol and erythromycin as the most effective antibi

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Fahrul, Muhammad, Ira Sari, and Dian Iriani. "Antibacterial Effectiveness of Seaweed (Eucheuma cottonii) Extract with Different Solvent." JURNAL AGROINDUSTRI HALAL 7, no. 1 (2021): 001–8. http://dx.doi.org/10.30997/jah.v7i1.3253.

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This research was aims to determine the antibacterial characteristics of seaweed extract Eucheuma cottonii with different solvents against bacteria Bacillus cereus and Salmonella typhi, to determine the diameter of the inhibition zone and the minimum inhibition concentration of Bacillus cereus and Salmonella typhi bacteria. The research method used was an experimental method by extraction of Eucheuma cottonii with different solvents, namely ethanol 96% and hexane. The analysis parameters consisted of phytochemical identification tested, antibacterial tested, inhibition zone diameter, and minim

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Mulyani, Yuniar, Karimah Syakirotin, Kiki Haetami, Yeni Mulyani, Evi Liviawaty, and Mochamad Untung Kurnia Agung. "Molecular Identification of Bacillus Derived from the Common Carp Intestine and Their Antibiotics Resistance." Jurnal Biodjati 5, no. 2 (2020): 294–302. http://dx.doi.org/10.15575/biodjati.v5i2.5263.

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The use of bacteria as a biological control agent in aquaculture is such new hope overcoming the negative impacts of the impropriate use of antibiotics. Bacillus is widely known as a potential probiotic for aquaculture, especially which are derived from aquatic biota. Due to a concern that the role of Bacillus as a probiotic agent will be disrupted by residues of antibiotics in the water, this research aimed to identify Bacillus species from common carp intestine based on the molecular method and investigate their resistance to some com-mercial antibiotics that widely used in aquaculture. Chlo

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Pereira, Débora Francine Gomes Silva, Silvia Nietsche, Adelica Aparecida Xavier, Suzane Ariádina de Souza, Márcia Regina Costa, and Anunciene Barbosa Duarte. "Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)." Revista Ceres 65, no. 5 (2018): 381–87. http://dx.doi.org/10.1590/0034-737x201865050001.

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ABSTRACT The objective of this study was to characterize banana tree endophytic bacteria at genus and species level and to determine the metabolic reactions associated with the nitrogen transformations. The identification at genus and species levels was performed using the partial sequencing of the rDNA 16S region. The assimbyotic nitrogen fixation, the reduction of nitrate and the production of urease were in vitro evaluated. The DNA of the bacterial isolates was also amplified to verify the presence of the nifH, nirK and nirS regions. Biochemical tests were performed in a complete randomized

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WANG, YING, TIANLI YUE, YAHONG YUAN, and ZHENPENG GAO. "Isolation and Identification of Thermo-Acidophilic Bacteria from Orchards in China." Journal of Food Protection 73, no. 2 (2010): 390–94. http://dx.doi.org/10.4315/0362-028x-73.2.390.

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Eight strains of thermo-acidophilic bacteria have been isolated from apple orchards in Shaanxi Province, China. The isolated strains were identified at the species level by comparing 16S rRNA gene sequences. It was found that all strains could be assigned to two genera. The strain YL-5 belonged to Alicyclobacillus, and other isolates belonged to Bacillus. The enzymatic patterns by the API ZYM system showed very significant differences between 12 strains of Alicyclobacillus and 8 strains of Bacillus. The ability of guaiacol production varied among different strains.

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Setiawan, Bagus, Didik Sulistyanto, and Kartika Senjarini. "Characterization Physiology and Molecular Bacteria Symbiont- Entomopathogenic Nematodes based of Gene Sequences Encoding the 16S rRNA District of Bromo Probolinggo." Jurnal ILMU DASAR 18, no. 1 (2017): 39. http://dx.doi.org/10.19184/jid.v18i1.2723.

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   This study aims to identify entomopathogenic nematodes symbiotic bacteria phenotypically and based on the gene encoding 16S rRNA sequences. Bacterial symbionts of entomopathogenic nematodes, obtained from isolates from the area Wonokerto (WN01) and isolates Sukapura (SP01), Bromo, Probolinggo, two symbiont bacteria was found in entomopathogenic nematodes Steinernema sp. The method used in this study are: the isolation of entomopathogenic nematodes Steinernema sp. and bacterial symbionts conventionally for the identification of phenotypically, after the characterization of

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Li, Kai, Liuhao Wang, Zhengyi Zhang, et al. "Isolation of Bacillus From the Gut of Bombus terrestris and Its Correlation in Queen Mating." Journal of Agricultural Science 13, no. 9 (2021): 155. http://dx.doi.org/10.5539/jas.v13n9p155.

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The gut of bumblebees harbors bacteria that play a crucial role in physiology, nutrition, and health. The mating rate is important for the reproductive activity of a colony; however, few studies have investigated the relationship between mating and gut bacteria. In this study, bacterial functions were identified in the intestinal tract of bumblebees, and biochemical identification and screening were performed using genetic detection technology. By isolating and identifying bacteria, we obtained a single strain and fed it to queens. The results indicated that Bacillus cereus and Bacillus pumilu

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Mardiah, Irma. "Identification of Endophytic Bacterial Isolated from Oil Palm Plants with Anti-Fungal Acitvity against Ganoderma boninense." Pharmacology and Clinical Pharmacy Research 3, no. 2 (2018): 41. http://dx.doi.org/10.15416/pcpr.v3i2.20005.

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Endophytic bacteria is an excellent candidates for the biological control of pathogenic fungi in plantations. The objectives of this study were to isolate and examine anti-fungal activity of endophytic bacteria from oil palm plantations in South Kalimantan against Ganoderma boninense. This research was performed in three phases, i.e., isolation, selection, and assessment of endophytic bacteria isolates against Ganoderma boninense. A total of 126 colonies of endophytic bacteria were isolated. The result of the anti-fungal activity test with dual culture method demostrated that BKA 10 isolate ha

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Hirata, T., K. Tosa, K. Kawamura, J. Nakajima, M. Kaneko, and K. Taguchi. "Heterotrophic Bacteria in Chlorinated Drinking Water Distribution Systems: Detection and Identification." Water Science and Technology 27, no. 3-4 (1993): 155–58. http://dx.doi.org/10.2166/wst.1993.0339.

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In order to determine bacterial quality of supplied water, researches on detection and identification of heterotrophic bacteria In distribution basin water and tap water were conducted In several drinking water supply systems In Japan, using pour plate technique with two agar media of SPG (Incubated at 36°C) and PGY (at 25°C). Bacterial colonies formed on SPC agar were randomly selected, purified and identified to genus. Results obtained from this study were as follow. The density of SPC bacteria ranged from 0 to 190 cfu/mL, although no colony was detected after 1-day Incubation in all of wate

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Pringgenies, Delianis, and Riyada Idris. "The antioxidant activity of carotenoid pigments in the bacterial symbionts of seagrass Syringodium isoetifolium." Indonesian Journal of Natural Pigments 1, no. 1 (2019): 12. http://dx.doi.org/10.33479/ijnp.2019.01.1.12.

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Carotenoids are pigments of red, yellow and orange which are found in plants, animals and bacteria, and are known to have antioxidant activity. This study aims to identify the carotenoid pigments detected in seagrass Syringodium isoetifolium bacterial symbionts. Isolation of bacteria was conducted using dispersive media Zobell 2116E. Bacterial isolates were cultured and then centrifuged at 8000 rpm for 10 minutes and extracted using methanol. Identification of the pigment was done by using High Performance Liquid Chromatography (HPLC) reversed phase ODS / C18. The mobile phase was carried out

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Setianingsih, Ika, Muhammad Rasyid Ridha, Syarif Hidayat, and Dicky Andiarsa. "Semut sebagai vektor mekanik bakteri di dalam gedung Balai Litbang P2B2 Tanah Bumbu, Kalimantan Selatan: studi pendahuluan." Journal of Health Epidemiology and Communicable Diseases 3, no. 2 (2019): 42–49. http://dx.doi.org/10.22435/jhecds.v3i2.1791.

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Ants are now now noticeable as a mechanical vector of pathogenic bacteria, some of which have been resistant to certain antibiotics. Its wide presence allows ants to easily transmit disease agents. This study aims to determine the type of ants in Balai Litbang P2B2 Tanah Bumbu and possible role as a vector mechanical bacteria. This research was conducted at Balai Litbang P2B2 Tanah Bumbu from August to September 2017, the sample was obtained from 15 rooms by using the sugar solution feed placed in the microtube, then the ant was identified by referring to Key to Identifying Common Household An

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Yu, Chen, Gao Hongwei, Zhang Yanming, et al. "Analysis of the Bacterial Diversity Existing on Animal Hide and Wool: Development of a Preliminary PCR-Restriction Fragment Length Polymorphism Fingerprint Database for Identifying Isolates." Journal of AOAC INTERNATIONAL 95, no. 6 (2012): 1750–54. http://dx.doi.org/10.5740/jaoacint.11-482.

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Abstract Twenty-one bacterial strains were isolated from imported cattle hide and rabbit wool using two types of media, nutrient broth, and nutrient broth with serum. The bacteria identified were Brevibacillus laterosporus, Leclercia adecarboxylata, Peptococcus niger, Bacillus circulans, Raoultella ornithinolytica, Bacillus subtilis, Bacillus cereus, Bacillus thermobacillus, Bacillus choshinensis, Bacillus sphaericus, Acinetobacter haemolyticus, Sphingomonas paucimobilis, Bacillus thuringiensis, Staphylococcus intermedius, Mycobacteria, Moraxella, Klebsiella pneumoniae, Ralstonia pickettii, St

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Sirvas Cornejo, Susana, Vanesa Buleje, Licia Salvatierra, and Michael L. Jaramillo. "Isolation and identification of proteolytic, amylolytic, lipolytic, and chitinolytic bacteria from shrimp waste." Revista Peruana de Biología 28, no. 1 (2021): e18353. http://dx.doi.org/10.15381/rpb.v28i1.18353.

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Bacteria and microbial enzymes are biocatalysts and can be used as an alternative to industrial chemical processes. The present study focused on isolating and identifying bacterial strains from shrimp waste, that produce amylases, lipases, proteases and chitinases with potential use on shrimp waste treatment. Thirty-two bacterial strains were isolated, phenotypically characterized, and identified by the API system and the molecular analysis of the 16S rDNA. It was found that 28.13% of the isolated bacterial strains had amylolytic capacity, 87.50% lipolytic, 96.88% proteolytic and 28.13% chitin

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Qian, Fang, Shu Juan Jiang, Tao Liu, and Guang Qing Mu. "Isolation and Identification of Thermostable Lactase-Producing Bacillus coagulans T242." Advanced Materials Research 634-638 (January 2013): 1253–58. http://dx.doi.org/10.4028/www.scientific.net/amr.634-638.1253.

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Lactase from the thermophilic bacteria has the good characteristics of heat-stability, high production and low pollution, and can effectively solve people’s problem of “lactose intolerance”. In the present work, a thermostable lactase-producing strain was isolated from the fermenting sample in fermenting tank of a spice factory in Chinese city of Dalian. Base on morphological observation, physiological and biochemical tests and molecular identification, the thermostable lactase-producing strain T242 was identified as Bacillus coagulans. Moreover, investigation revealed that lactase from Bacill

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Onawola, O. O., I. S. Akande, W. O. Okunowo, and A. A. Osuntoki. "Isolation and identification of phytase-producing Bacillus and Enterobacter species from Nigerian soils." Nigerian Journal of Biotechnology 36, no. 2 (2020): 127–38. http://dx.doi.org/10.4314/njb.v36i2.13.

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The bioavailability of phosphorus and other nutrients in feed for monogastrics is restricted by the presence of phytate. Exogenous supply of phytase produced by bacteria and other microbes can enhance the bioavailability of these nutrients and reduce phosphorus pollution. The objective of the study was to isolate bacteria with phytase-producing potentials from soil; which may be employed for the bioavailability of phosphorus and other nutrients in feed for monogastrics in Nigeria. Top soil samples were collected from two dumpsites in Lagos, Nigeria and bacteria were isolated and screened for p

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Melo, Flávia Mandolesi Pereira de, Marli Fátima Fiore, Luiz Alberto Beraldo de Moraes, et al. "Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a." Scientia Agricola 66, no. 5 (2009): 583–92. http://dx.doi.org/10.1590/s0103-90162009000500002.

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In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythi

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Sangi, Fahimeh, Abbas Mohammadi, Nazar Afzali3, and Mansoureh Mirabolfathy. "Identification of New Aflatoxin B1-Degrading Bacteria from Iran." Iranian Journal of Toxicology 12, no. 3 (2018): 39–44. http://dx.doi.org/10.32598/ijt.12.3.507.1.

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Background: Aflatoxin B1 (AFB1) is a mutagenic and carcinogenic compound mainly produced by the Aspergillus parasiticus, A. flavus, A. nomius, A. tamari, and A. pseudotamarii. AFB1 biodegradation is the most important strategy for reducing AFB1 in plant tissues. Bacteria can deactivate and biodegrade AFB1 for effective detoxification of contaminated products. The present study investigated the efficiency of AFB1 degradation by soil bacteria from the Southern Khorasan Province in Eastern Iran by thin-layer and high-performance liquid chromatography during 2014–2015. Methods: DNA was extracted f

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Deng, Jing, Qijue Chen, Boyong Hu, et al. "Synergic Effect of Adsorption and Biodegradation by Microsphere Immobilizing Bacillus velezensis for Enhanced Removal Organics in Slaughter Wastewater." Processes 9, no. 7 (2021): 1145. http://dx.doi.org/10.3390/pr9071145.

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Bacterial cell immobilization offers considerable advantages over traditional biotreatment methods using free bacteria. Bacillus velezensis was underwented isolation and genetic identification as COD-degrading bacteria in slaughter wastewaterand immobilized on the surface of polyvinyl alcohol (PVA) microsphere with the adhesion to bio-carrier through direct physical adsorption. The removal CODMn rates of microsphere (PVA) immobilized cells were 16.99%, increased 9.38% from a 50% concentration of slaughter wastewater within 24 h at 37 °C, pH 7.0, and 120 rpm, which was about 2.2 times that of t

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