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Journal articles on the topic 'Bacillus cereus Bihar region'

Author: Grafiati

Published: 3 June 2025

Last updated: 1 August 2025

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1

Xu, Dong, and Jean-Charles Côté. "Sequence Diversity of the Bacillus thuringiensis and B. cereus Sensu Lato Flagellin (H Antigen) Protein: Comparison with H Serotype Diversity." Applied and Environmental Microbiology 72, no. 7 (2006): 4653–62. http://dx.doi.org/10.1128/aem.00328-06.

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ABSTRACT We set out to analyze the sequence diversity of the Bacillus thuringiensis flagellin (H antigen [Hag]) protein and compare it with H serotype diversity. Some other Bacillus cereus sensu lato species and strains were added for comparison. The internal sequences of the flagellin (hag) alleles from 80 Bacillus thuringiensis strains and 16 strains from the B. cereus sensu lato group were amplified and cloned, and their nucleotide sequences were determined and translated into amino acids. The flagellin allele nucleotide sequences for 10 additional strains were retrieved from GenBank for a

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2

NAKANO, SHIGERU, HIDEKI MAESHIMA, ATSUSHI MATSUMURA, et al. "A PCR Assay Based on a Sequence-Characterized Amplified Region Marker for Detection of Emetic Bacillus cereus." Journal of Food Protection 67, no. 8 (2004): 1694–701. http://dx.doi.org/10.4315/0362-028x-67.8.1694.

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A PCR assay for the detection of Bacillus cereus strains able to produce an emetic toxin (cereulide) was developed in this study based on a sequence-characterized amplified region (SCAR) derived from a random amplified polymorphic DNA (RAPD) fragment. One of the RAPD fragments generated was selected, cloned, and sequenced. A set of PCR primers was newly designed from the SCAR obtained (the sequence of the cloned RAPD fragment) and used in this assay. To determine the specificity of the assay, 30 different B. cereus strains, 8 other Bacillus strains (of six species), and 16 other non-Bacillus s

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3

Worthy, Harley L., Lainey J. Williamson, Husam Sabah Auhim, et al. "The Crystal Structure of Bacillus cereus HblL1." Toxins 13, no. 4 (2021): 253. http://dx.doi.org/10.3390/toxins13040253.

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The Hbl toxin is a three-component haemolytic complex produced by Bacillus cereus sensu lato strains and implicated as a cause of diarrhoea in B. cereus food poisoning. While the structure of the HblB component of this toxin is known, the structures of the other components are unresolved. Here, we describe the expression of the recombinant HblL1 component and the elucidation of its structure to 1.36 Å. Like HblB, it is a member of the alpha-helical pore-forming toxin family. In comparison to other members of this group, it has an extended hydrophobic beta tongue region that may be involved in

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Slamti, Leyla, Stéphane Perchat, Myriam Gominet, et al. "Distinct Mutations in PlcR Explain Why Some Strains of the Bacillus cereus Group Are Nonhemolytic." Journal of Bacteriology 186, no. 11 (2004): 3531–38. http://dx.doi.org/10.1128/jb.186.11.3531-3538.2004.

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ABSTRACT Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis are closely related species belonging to the Bacillus cereus group. B. thuringiensis and B. cereus generally produce extracellular proteins, including phospholipases and hemolysins. Transcription of the genes encoding these factors is controlled by the pleiotropic regulator PlcR. Disruption of plcR in B. cereus and B. thuringiensis drastically reduces the hemolytic, lecithinase, and cytotoxic properties of these organisms. B. anthracis does not produce these proteins due to a nonsense mutation in the plcR gene. We screene

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Metleva, Anastasia, and Oksana Smolovskaya. "Epizootological data of cattle infection caused by bacillus cereus on a dairy farm of the Kemerovo region." E3S Web of Conferences 380 (2023): 01006. http://dx.doi.org/10.1051/e3sconf/202338001006.

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Diseases caused by Bacillus cereus of non-food origin are becoming relevant. This article describes a case of an epizootic situation associated with the spread of B. cereus through cattle feed and its ubiquitous distribution to all environmental objects and animal biotopes on the farm. B. cereus can cause not only food poisoning but also a generalized form of the infectious process, characterized by severe intestinal stagnation with a thickened and edematous wall, hemorrhages on the mucosal surface, catarrhal enteritis, systemic lymphadenopathy, degeneration of muscle fibers, interstitial pneu

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Xu, Bowen, Xinyi Huang, Haixiong Qin, et al. "Evaluating the Safety of Bacillus cereus GW-01 Obtained from Sheep Rumen Chyme." Microorganisms 12, no. 7 (2024): 1457. http://dx.doi.org/10.3390/microorganisms12071457.

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Bacillus cereus is responsible for 1.4–12% food poisoning outbreaks worldwide. The safety concerns associated with the applications of B. cereus in health and medicine have been controversial due to its dual role as a pathogen for foodborne diseases and a probiotic in humans and animals. In this study, the pathogenicity of B. cereus GW-01 was assessed by comparative genomic, and transcriptome analysis. Phylogenetic analysis based on a single-copy gene showed clustering of the strain GW-01, and 54 B. cereus strains from the NCBI were classified into six major groups (I–VI), which were then asso

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Todd, Sarah J., Arthur J. G. Moir, Matt J. Johnson, and Anne Moir. "Genes of Bacillus cereus and Bacillus anthracis Encoding Proteins of the Exosporium." Journal of Bacteriology 185, no. 11 (2003): 3373–78. http://dx.doi.org/10.1128/jb.185.11.3373-3378.2003.

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ABSTRACT The exosporium is the outermost layer of spores of Bacillus cereus and its close relatives Bacillus anthracis and Bacillus thuringiensis. For these pathogens, it represents the surface layer that makes initial contact with the host. To date, only the BclA glycoprotein has been described as a component of the exosporium; this paper defines 10 more tightly associated proteins from the exosporium of B. cereus ATCC 10876, identified by N-terminal sequencing of proteins from purified, washed exosporium. Likely coding sequences were identified from the incomplete genome sequence of B. anthr

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Dey, Uttiya, Naba Kumar Mondal, Soumendranath Chatterjee, et al. "The Isolation and Identification of Novel Arsenic-Resistant Bacteria from an Arsenic-Contaminated Region—A Study to Understand the Efficiency of Bacteria for Arsenic Removal from Aqueous Media." Water 16, no. 17 (2024): 2401. http://dx.doi.org/10.3390/w16172401.

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Drinking water sources with groundwater arsenic (As) contamination face multifaceted challenges in the removal and supply of fresh drinking water resources. To eradicate this problem, bioremediation has evolved to become more effective than other chemical and physical removal processes in its cost-effectiveness, high removal efficiency, and lesser production of secondary by-products or waste. Thus, this study aimed to treat As from aqueous media and to detoxify highly toxic forms of As by the isolated bacteria from As-affected areas. We isolated two new Gram-positive bacteria, which are report

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9

MESSELHÄUSSER, U., P. KÄMPF, M. FRICKER, et al. "Prevalence of Emetic Bacillus cereus in Different Ice Creams in Bavaria." Journal of Food Protection 73, no. 2 (2010): 395–99. http://dx.doi.org/10.4315/0362-028x-73.2.395.

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In this study, 809 samples of ice cream from different sources were investigated by using cultural methods for the presence of presumptive Bacillus cereus. Isolates from culture-positive samples were examined with a real-time PCR assay targeting a region of the cereulide synthetase gene (ces) that is highly specific for emetic B. cereus strains. The samples were collected from ice cream parlors and restaurants that produced their own ice cream and from international commercial ice cream companies in different regions of Bavaria during the summer of 2008. Presumptive B. cereus was found in 508

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Al-Thobaiti, Bander Mohammed, Emad Abada, and Khaled El-Gayar. "Biochemical and molecular characterization of proteolytic bacterial strains isolated from Jazan region, KSA with the application as an antibacterial agent." Modern Journal of Microbial Biology 3 (April 7, 2020): 1. http://dx.doi.org/10.25259/mjmb_1_2021.

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Objectives: Biochemical and molecular characterization of proteolytic bacterial strains isolated from Jazan region, KSA with the application as an antibacterial agent. Materials and Methods: Three samples were collected from extreme environment, Jazan, KSA. Skim milk nutrient agar medium was used for protease screening for several colonies by streaking method at 37°C. API biochemical kit was used to characterize the three isolates using some selective media. The genetic identification was done using 16S rRNA gene sequencing. The sensitivity of the tested strains;Staphylococcus aureus, Escheric

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Silfiani, Intan, Abdul Latief Abadi, Luqman Qurata Aini, Kestrillia Rega Prillianti, Matheus Randy Prabowo, and Sylvia Herli Dianti. "FTIR (Fourier Transform Infrared) Spectroscopy Method for Bacillus cereus Characterization." Research Journal of Life Science 9, no. 2 (2022): 69–76. http://dx.doi.org/10.21776/ub.rjls.2022.009.02.3.

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One of the potentials of Bacillus cereus bacteria is as an inducer of systemic resistance in plants against plant diseases. FTIR spectroscopy is a traditional method extensively utilized in the pharmaceutical industry, since it is fast, non-destructive, and requires minimal sample preparation. In this study, the indicators that were effectively discovered were the functional groups that compose carbohydrates and lipids. The absorption region to be analyzed for the functional groups that compose carbohydrates is in the wavenumber region of 1200-800 cm-1, whereas the functional groups that compo

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Andreeva, Irina S., Aleksandr S. Safatov, Larisa I. Puchkova, et al. "Occurrence and characteristics of Bacillus cereus group bacterial atmospheric aerosols in Novosibirsk region." Vestnik Tomskogo gosudarstvennogo universiteta. Biologiya, no. 56 (2021): 60–85. http://dx.doi.org/10.17223/19988591/56/3.

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The microbial diversity of atmospheric bioaerosols involves microorganisms that can cause allergic and infectious diseases or toxic effects. They include bacteria of the Bacillus cereus group (B. cereus, B. thuringiensis, B. anthracis, B. mycoides, B. pseudomycoides, etc.), which can result in diarrhea, pneumonia, meningitis, septicemia, and other infectious diseases. Accordingly, monitoring the presence of Bacillus cereus group bacteria in aerosols is critical. However, practically no data exist on Bacillus cereus and other cereus-group bacteria in southwestern Siberia’s poorly investigated a

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Emmert, Elizabeth A. B., Amy K. Klimowicz, Michael G. Thomas, and Jo Handelsman. "Genetics of Zwittermicin A Production by Bacillus cereus." Applied and Environmental Microbiology 70, no. 1 (2004): 104–13. http://dx.doi.org/10.1128/aem.70.1.104-113.2004.

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ABSTRACT Zwittermicin A represents a new chemical class of antibiotic and has diverse biological activities, including suppression of oomycete diseases of plants and potentiation of the insecticidal activity of Bacillus thuringiensis. To identify genes involved in zwittermicin A production, we generated 4,800 transposon mutants of B. cereus UW101C and screened them for zwittermicin A accumulation. Nine mutants did not produce detectable zwittermicin A, and one mutant produced eightfold more than the parent strain. The DNA flanking the transposon insertions in six of the nine nonproducing mutan

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Carlson, Cathrine Rein, and Anne-Brit Kolsto. "A small (2.4 Mb) Bacillus cereus chromosome corresponds to a conserved region of a larger (5.3 Mb) Bacillus cereus chromosome." Molecular Microbiology 13, no. 1 (1994): 161–69. http://dx.doi.org/10.1111/j.1365-2958.1994.tb00411.x.

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Zahner, Viviane, Diana Aparecida Cabral, Adriana Hamond Régua-Mangia, Leon Rabinovitch, Gaétan Moreau, and Douglas McIntosh. "Distribution of Genes Encoding Putative Virulence Factors and Fragment Length Polymorphisms in the vrrA Gene among Brazilian Isolates of Bacillus cereus and Bacillus thuringiensis." Applied and Environmental Microbiology 71, no. 12 (2005): 8107–14. http://dx.doi.org/10.1128/aem.71.12.8107-8114.2005.

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ABSTRACT One hundred twenty-one strains of the Bacillus cereus complex, of which 80 were isolated from a variety of sources in Brazil, were screened by PCR for the presence of sequences (bceT, hblA, nheBC, plc, sph, and vip3A) encoding putative virulence factors and for polymorphisms in variable-number tandem repeats (VNTR), using a variable region of the vrrA open reading frame as the target. Amplicons were generated from isolates of B. cereus and Bacillus thuringiensis for each of the sequences encoding factors suggested to play a role in infections of mammals. Intriguingly, the majority of

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Harvie, Duncan R., Susana Vílchez, James R. Steggles, and David J. Ellar. "Bacillus cereus Fur regulates iron metabolism and is required for full virulence." Microbiology 151, no. 2 (2005): 569–77. http://dx.doi.org/10.1099/mic.0.27744-0.

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A homologue of the Bacillus subtilis fur gene was identified in Bacillus cereus and characterized. The predicted amino acid sequence of the cloned gene was found to be highly similar to other members of the Fur family of transcriptional regulators. The B. cereus fur gene was shown to partially complement an Escherichia coli fur mutant. Purified B. cereus Fur bound specifically to a 19 bp DNA sequence homologous to the B. subtilis Fur box in a metal-dependent manner. Analysis of the available B. cereus genome data identified a number of genes which contain predicted Fur box sequences in the pro

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Catania, Angela Maria, Tiziana Civera, Pierluigi Aldo Di Ciccio, Maria Ausilia Grassi, Patrizia Morra, and Alessandra Dalmasso. "Characterization of Vegetative Bacillus cereus and Bacillus subtilis Strains Isolated from Processed Cheese Products in an Italian Dairy Plant." Foods 10, no. 11 (2021): 2876. http://dx.doi.org/10.3390/foods10112876.

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Processed cheese is a commercial product characterized by high microbiological stability and extended shelf life obtained through the application of severe heat treatment. However, spore-forming bacteria can survive through thermal processes. Among them, microorganisms belonging to Bacillus genus have been reported. In this study, we examined the microbiological population of the first hours’ production of processed cheeses in an Italian dairy plant during two seasons, between June and October 2020. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was

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Owusu-Darko, Rodney, Mushal Allam, Arshad Ismail, Carlos A. S. Ferreira, Sílvia D. de Oliveira, and Elna M. Buys. "Comparative Genome Analysis of Bacillus sporothermodurans with Its Closest Phylogenetic Neighbor, Bacillus oleronius, and Bacillus cereus and Bacillus subtilis Groups." Microorganisms 8, no. 8 (2020): 1185. http://dx.doi.org/10.3390/microorganisms8081185.

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Bacillus sporothermodurans currently possesses one of the most highly heat-resistant spores (HRS), which can withstand ultra-high temperature (UHT) processing. Determination of multiple whole genome sequences of B. sporothermodurans provided an opportunity to perform the first comparative genome analysis between strains and with B. oleronius, B. cereus, and B. subtilis groups. In this study, five whole genome sequences of B. sporothermodurans strains, including those belonging to the HRS clone (SAD and BR12) normally isolated from UHT milk, were compared with the aforementioned Bacillus specie

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Koo, Kai, Peggy M. Foegeding, and Harold E. Swaisgood. "Construction and Expression of a Bifunctional Single-Chain Antibody against Bacillus cereusSpores." Applied and Environmental Microbiology 64, no. 7 (1998): 2490–96. http://dx.doi.org/10.1128/aem.64.7.2490-2496.1998.

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ABSTRACT The variable-region genes of monoclonal antibody againstBacillus cereus spores were cloned from mouse hybridoma cells by reverse transcription-PCR. The heavy- and light-chain variable-region genes were connected by a 45-base linker DNA to allow folding of the fusion protein into a functional tertiary structure. For detection of protein expression, a 10-amino-acid strep tag (biotin-like peptide) was attached to the C terminus of recombinant antibody as the reporter peptide. The single-chain antibody construct was inserted into the expression vector and expressed in Escherichia coliunde

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Ibraheem, Khalida Jhalil. "Antimicrobial Activity of Extracts Bacillus Species Isolated From Baghdad Soil Against Some Human Pathogenic Microorganisms." Al Mustansiriyah Journal of Pharmaceutical Sciences 16, no. 2 (2016): 82–87. http://dx.doi.org/10.32947/ajps.v16i2.114.

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Bacillus species are the predominant soil bacteria because of their resistant endospore formation and can produce many different antimicrobial substances. The main aim of this study was to isolate Bacillus species from soil and investigate their antimicrobial activity against some pathogenic bacteria and fungi isolated from human. 48 soil samples were collected from different region of Baghdad city (Rashidiya, Mahmudiyah, Alkraat and Aldora) during December 2015 and analyzed for the presence of Bacillus species. Bacterial isolates were identified by using different microscopical ex

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Nagel, Alexey S., Olesya S. Vetrova, Natalia V. Rudenko та ін. "A High-Homology Region Provides the Possibility of Detecting β-Barrel Pore-Forming Toxins from Various Bacterial Species". International Journal of Molecular Sciences 25, № 10 (2024): 5327. http://dx.doi.org/10.3390/ijms25105327.

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The pathogenicity of many bacteria, including Bacillus cereus and Staphylococcus aureus, depends on pore-forming toxins (PFTs), which cause the lysis of host cells by forming pores in the membranes of eukaryotic cells. Bioinformatic analysis revealed a region homologous to the Lys171-Gly250 sequence in hemolysin II (HlyII) from B. cereus in over 600 PFTs, which we designated as a “homologous peptide”. Three β-barrel PFTs were used for a detailed comparative analysis. Two of them—HlyII and cytotoxin K2 (CytK2)—are synthesized in Bacillus cereus sensu lato; the third, S. aureus α-toxin (Hla), is

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Schwenk, Valerie, Richard Dietrich, Andreas Klingl, Erwin Märtlbauer, and Nadja Jessberger. "Characterization of strain-specific Bacillus cereus swimming motility and flagella by means of specific antibodies." PLOS ONE 17, no. 3 (2022): e0265425. http://dx.doi.org/10.1371/journal.pone.0265425.

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One of the multiple factors determining the onset of the diarrhoeal disease caused by enteropathogenic Bacillus cereus is the ability of the bacteria to actively move towards the site of infection. This ability depends on flagella, but it also varies widely between different strains. To gain more insights into these strain-specific variations, polyclonal rabbit antisera as well as monoclonal antibodies (mAbs) were generated in this study, which detected recombinant and natural B. cereus flagellin proteins in Western blots as well as in enzyme immunoassays (EIAs). Based on mAb 1A11 and HRP-labe

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BARON, FLORENCE, MARIE-FRANÇOISE COCHET, NOËL GROSSET, et al. "Isolation and Characterization of a Psychrotolerant Toxin Producer, Bacillus weihenstephanensis, in Liquid Egg Products." Journal of Food Protection 70, no. 12 (2007): 2782–91. http://dx.doi.org/10.4315/0362-028x-70.12.2782.

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A psychrotolerant bacteria of the Bacillus cereus group was found responsible for the spoilage of whole liquid egg products. By sequencing a 16S rRNA region and performing a PCR amplification of specific 16S rRNA and cspA signatures, a Bacillus weihenstephanensis was identified. Characterization of this strain shows its ability to grow in defined medium as well as in whole liquid egg at refrigerated temperatures. The strain isolated possesses genes encoding for hemolysin BL, nonhemolytic enterotoxin, and B. cereus enterotoxins and produces enterotoxins with cytotoxic activity in whole liquid e

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Kayath, Christian Aimé, Saturnin Nicaise Mokemiabeka, and Nicole Prisca Makaya Dangui Nieko. "Correlation of Mutations in the hblA Gene with Bacillus cereus Disease Isolated from Loungwila, a Traditional Fermented Beverages in Republic of Congo." Journal of Advances in Microbiology 25, no. 6 (2025): 38–45. https://doi.org/10.9734/jamb/2025/v25i6947.

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Bacillus cereus is a pathogenic bacterium recognized for triggering foodborne illnesses, particularly through its enterotoxins, which are linked to various gastroenteritis outbreaks. This study explores the relationship between mutations in the hblA gene, which encodes for the haemolysin BL toxin, and B. cereus strains isolated from Lougwila, a traditional fermented beverage in the Republic of Congo. A total of 145 B. cereus strains were obtained from Lougwila samples, and their hblA gene sequences were analyzed for mutations by using PCR, sequencing and in silico translation. In the hblA regi

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Sun, Yang, Wenjing Shuai, Lanmengya Nie, Xiangfei Li, and Ling Jiang. "Investigating the Role of OrbF in Biofilm Biosynthesis and Regulation of Biofilm-Associated Genes in Bacillus cereus BC1." Foods 13, no. 5 (2024): 638. http://dx.doi.org/10.3390/foods13050638.

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Bacillus cereus (B. cereus), a prevalent foodborne pathogen, constitutes a substantial risk to food safety due to its pronounced resilience under adverse environmental conditions such as elevated temperatures and ultraviolet radiation. This resilience can be attributed to its capacity for biofilm synthesis and sustained high viability. Our research aimed to elucidate the mechanisms governing biofilm biosynthesis in B. cereus. To this end, we constructed a 5088-mutant library of the B. cereus strain BC1 utilizing the transposon TnYLB-1. Systematic screening of this library yielded mutants exhib

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Li, Tingting, Qinpei Zou, Cheng Chen, et al. "A foodborne outbreak linked to Bacillus cereus at two middle schools in a rural area of Chongqing, China, 2021." PLOS ONE 18, no. 10 (2023): e0293114. http://dx.doi.org/10.1371/journal.pone.0293114.

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Bacillus cereus (B. cereus) is a common cause of foodborne illness. An outbreak of acute gastrointestinal illness occurred at two middle schools in a rural region of Chongqing, China, in 2021. This study aimed to elucidate the outbreak’s characteristics, identify risk factors, and determine the source of contamination. A retrospective cohort study and an environmental investigation were conducted. Vomit samples, anal swabs, and food samples were collected and tested by RT-PCR for 18 species of bacteria and viruses, including B. cereus. Positive samples of B. cereus underwent biochemical experi

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Chakraborty, Brinta, Anish Mallick, Sumana Annagiri, Supratim Sengupta, and Tapas K. Sengupta. "Deciphering a survival strategy during the interspecific competition between Bacillus cereus MSM-S1 and Pseudomonas sp. MSM-M1." Royal Society Open Science 3, no. 11 (2016): 160438. http://dx.doi.org/10.1098/rsos.160438.

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Interspecific competition in bacteria governs colony growth dynamics and pattern formation. Here, we demonstrate an interesting phenomenon of interspecific competition between Bacillus cereus MSM-S1 and Pseudomonas sp . MSM-M1, where secretion of an inhibitor by Pseudomonas sp . is used as a strategy for survival. Although B. cereus grows faster than Pseudomonas sp., in the presence of Pseudomonas sp. the population of B. cereus reduces significantly, whereas Pseudomonas sp. do not show any marked alteration in their population growth. Appearance of a zone of inhibition between growing colonie

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Goncharova, Y. O., V. V. Evseeva, R. I. Mironova, et al. "Characteristics of <i>Bacillus cereus</i> complex Group Strains Isolated from Permafrost in Yakutia for Assessment of Microbiological Risks During Climate Change." Прикладная биохимия и микробиология 59, no. 6 (2023): 589–98. http://dx.doi.org/10.31857/s0555109923060053.

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Strains of Bacillus genus were isolated from soil samples in the permafrost region (Yakutia, Russia). The phenotypic characteristics of the strains are given. The analysis of the obtained data made it possible to assign them to the group Bacillus cereus complex. PCR analysis made it possible to determine the profile of B. cereus toxin synthesis genes in the genomes of the studied strains. Genetic characterization was obtained by RAPD genotyping and using MLVA loci used for genotyping of the anthrax pathogen. The results of genotyping at different levels of resolution made it possible to differ

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Bianco, Angelica, Giovanni Normanno, Loredana Capozzi, et al. "High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy." Foods 12, no. 7 (2023): 1548. http://dx.doi.org/10.3390/foods12071548.

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The Bacillus cereus group includes species that act as food-borne pathogens causing diarrheal and emetic symptoms. They are widely distributed and can be found in various foods. In this study, out of 550 samples of milk and cheeses, 139 (25.3%) were found to be contaminated by B. cereus sensu lato (s.l.). One isolate per positive sample was characterized by Multilocus Sequence Typing (MLST) and for the presence of ten virulence genes. Based on MLST, all isolates were classified into 73 different sequence types (STs), of which 12 isolates were assigned to new STs. Virulence genes detection reve

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Brillard, Julien, and Didier Lereclus. "Comparison of cytotoxin cytK promoters from Bacillus cereus strain ATCC 14579 and from a B. cereus food-poisoning strain." Microbiology 150, no. 8 (2004): 2699–705. http://dx.doi.org/10.1099/mic.0.27069-0.

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The cytotoxin CytK produced by Bacillus cereus is believed to be involved in food-borne diseases. The transcriptional activity of the cytK promoter region in a food-poisoning strain was studied using a reporter gene and compared with that in the reference B. cereus strain ATCC 14579. In the food-poisoning strain, cytK is more strongly transcribed, possibly explaining the pathogenicity. The global regulator PlcR in B. cereus controls several putative virulence factors. It was found that PlcR regulates cytK in this clinical strain despite a mismatch in the PlcR recognition site, as currently def

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Hansen, Bjarne Munk, and Niels Bohse Hendriksen. "Detection of Enterotoxic Bacillus cereus andBacillus thuringiensis Strains by PCR Analysis." Applied and Environmental Microbiology 67, no. 1 (2001): 185–89. http://dx.doi.org/10.1128/aem.67.1.185-189.2001.

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ABSTRACT Many strains of Bacillus cereus cause gastrointestinal diseases, and the closely related insect pathogen B. thuringiensis has also been involved in outbreaks of diarrhea. The diarrheal types of diseases are attributed to enterotoxins. Two different enterotoxic protein complexes, hemolysin BL (HBL) and nonhemolytic enterotoxin (NHE), and an enterotoxic protein, enterotoxin T, have been characterized, and the genes have been sequenced. PCR primers for the detection of these genes were deduced and used to detect the genes in 22 B. cereus and 41 B. thuringiensis strains. At least one gene

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Zerhouni, K., B. Abbouni, K. Kanoun, et al. "Isolation and identification of low density polythene-degrading bacteria from soil of North West of Algeria." South Asian Journal of Experimental Biology 8, no. 3 (2019): 76–82. http://dx.doi.org/10.38150/sajeb.8(3).p76-82.

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Plas c bags (Low Density Polyethylene (LDPE) belong to the polymers, which plays a very important role in our daily lives by their diversi ed applica on. However, the accumula on of the plas c bags in the environment cons - tutes a serious problem and a real source for visual nuisance, pollu on of soil and marine environments. Furthermore, their biodegradation was the safest method of breakdown that possibly leaves behind less toxic residues and showed poten al of bio-geo chemical cycling of the substrate. The aim of the present work was the characterization of the isolated bacterial strains f

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Guermoudi, S., M. Ziane, and M. Bouamra. "Modeling of Bacillus cereus evolution in pasteurized cow milk locally distributed at point of sale: a case of three companies in Tlemcen region, Algeria." Food Research 8, no. 5 (2024): 310–18. http://dx.doi.org/10.26656/fr.2017.8(5).590.

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Bacillus cereus is among the most hazardous bacteria that could reduce the shelf life of pasteurized milk and could threaten consumers’ health. These bacteria groups are known for their heat resistance and their ability to produce two types of toxins, diarrheic and emetic. This study aimed to enumerate the B. cereus in raw cow’s milk collected from three companies in the Tlemcen region, then modeled, based on a probabilistic approach, their evolution throughout the process, split into six steps, until the point of sale. At first, the analysis of 242 samples on MYP medium, shows a high prevalen

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Kong, Minsuk, and Sangryeol Ryu. "Bacteriophage PBC1 and Its Endolysin as an Antimicrobial Agent against Bacillus cereus." Applied and Environmental Microbiology 81, no. 7 (2015): 2274–83. http://dx.doi.org/10.1128/aem.03485-14.

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ABSTRACTBacillus cereusis an opportunistic human pathogen responsible for food poisoning and other, nongastrointestinal infections. Due to the emergence of multidrug-resistantB. cereusstrains, the demand for alternative therapeutic options is increasing. To address these problems, we isolated and characterized aSiphoviridaevirulent phage, PBC1, and its lytic enzymes. PBC1 showed a very narrow host range, infecting only 1 of 22B. cereusstrains. Phylogenetic analysis based on the major capsid protein revealed that PBC1 is more closely related to theBacillus clarkiiphage BCJA1c and phages of lact

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Rao, Shilpakala Sainath, Ketha V. K. Mohan, and Chintamani D. Atreya. "High Sensitivity Detection of Bacillus Cereus in Plasma Samples." Blood 112, no. 11 (2008): 1990. http://dx.doi.org/10.1182/blood.v112.11.1990.1990.

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Abstract Microbial contamination of blood and blood products remains a major concern in transfusion medicine. Detection of bacterial contamination has been traditionally carried out by inoculating the contaminated blood/blood products into a liquid or solid nutrient medium followed by 18–24 h incubation period to observe any bacterial colony formation (growth). Although this technique is highly sensitive and specific, the test requires at least 24 hours for accurate diagnosis, which is a limitation Nucleic acid-based and antigen-based assays that have been recently in use also have limitations

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СУЩИХ, В. Ю., М. Р. ЮСУПОВ, Б. КАНАТОВ, С. М. ДЮСЕНОВ, and А. А. КАРИМОВ. "EPIZOOTOLOGICAL AND MICROBIOLOGICAL MONITORING OF SOIL ANTHRAX FOCUSES LOCATED IN AKMOLA REGION." МИКРОБИОЛОГИЯ ЖӘНЕ ВИРУСОЛОГИЯ, no. 1(40) (March 16, 2023): 180–91. http://dx.doi.org/10.53729/mv-as.2023.01.12.

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Почвенные очаги сибирской язвы, расположенные на территории Республики Казахстан, представляют опасность для населения и окружающей среды. Согласно ретроспективным данным, в августе 2019 года в селе Ольгинка Аршалинского района Акмолинской области была зарегистрирована вспышка сибирской язвы, при этом заболели 2 головы крупного рогатого скота. Осуществлены выезды в село Николаевка, где расположены два сибиреязвенных захоронения, и в село Ольгинка, на территорию мест убоя больных животных. В процессе работы проведено эпизоотологическое обследование двух сибиреязвенных очагов и мест прирезки бол

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Bailey-Smith, Karen, Sarah J. Todd, Thomas W. Southworth, John Proctor, and Anne Moir. "The ExsA Protein of Bacillus cereus Is Required for Assembly of Coat and Exosporium onto the Spore Surface." Journal of Bacteriology 187, no. 11 (2005): 3800–3806. http://dx.doi.org/10.1128/jb.187.11.3800-3806.2005.

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ABSTRACT The outermost layer of spores of the Bacillus cereus family is a loose structure known as the exosporium. Spores of a library of Tn917-LTV1 transposon insertion mutants of B. cereus ATCC 10876 were partitioned into hexadecane; a less hydrophobic mutant that was isolated contained an insertion in the exsA promoter region. ExsA is the equivalent of SafA (YrbA) of Bacillus subtilis, which is also implicated in spore coat assembly; the gene organizations around both are identical, and both proteins contain a very conserved N-terminal cortex-binding domain of ca. 50 residues, although the

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Ifediegwu, Marcel Chikelue, Michael Uchenna Orji, Samuel Chinedu Onuorah, and Bright Obidinma Uba. "Evaluation of the degrading potentials of plasmid and non–plasmid borne soil bacterial strains on bonny light crude oil." Archives of Agriculture and Environmental Science 9, no. 1 (2024): 14–22. http://dx.doi.org/10.26832/24566632.2024.090103.

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This study was undertaken to evaluate the degrading potentials of plasmid and non–plasmid borne soil bacterial strains on Bonny light crude oil. Enrichment technique, turbidometric test, plasmid curing test as well as gas chromatographic flame ionization detection technique was adopted for isolating and evaluating the oil degrading capabilities of the selected bacterial strains. The preliminary physicochemical results revealed that pH was recorded slightly neutral, higher conductivity (0.41 to 0.44 μS/cm), higher organic carbon (2.32 to 4.34 %) but lower nitrogen and phosphorus contents (0.27

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Lücking, Genia, Monica K. Dommel, Siegfried Scherer, Agnes Fouet, and Monika Ehling-Schulz. "Cereulide synthesis in emetic Bacillus cereus is controlled by the transition state regulator AbrB, but not by the virulence regulator PlcR." Microbiology 155, no. 3 (2009): 922–31. http://dx.doi.org/10.1099/mic.0.024125-0.

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Cereulide, a depsipeptide structurally related to the antibiotic valinomycin, is responsible for the emetic type of gastrointestinal disease caused by Bacillus cereus. Recently, it has been shown that cereulide is produced non-ribosomally by the plasmid-encoded peptide synthetase Ces. Using deletion mutants of the emetic reference strain B. cereus F4810/72, the influence of the well-known transcription factors PlcR, Spo0A and AbrB on cereulide production and on the transcription of the cereulide synthetase gene cluster was investigated. Our data demonstrate that cereulide synthesis is independ

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S., Padmavathy, K. Asha Devi N., and S. Bhuvaneshwari D. "Biosorption of cyanide using Bacillus cereus from Salt Pan, Tuticorin, Tamilnadu – Isotherm and kinetic studies." Journal of Indian Chemical Society Vol. 93, Feb 2016 (2016): 211–22. https://doi.org/10.5281/zenodo.5603142.

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Department of Zoology and Microbiology, Thiagarajar College, Madurai-625 009, Tamilnadu, India Department of Chemistry, Thiagarajar College, Madurai-625 009, Tamilnadu, India <em>E-mail </em>: bukanch@yahoo.co.in <em>Manuscript received online 14 February 2015, revised 07 June 2015, accepted 09 June 2015</em> In the present study, with the aim of isolating cyanide degrading organisms, four different Gram-positive, aerobic, endospore-forming bacteria (S1 to S4) having the ability to degrade cyanide were isolated by enrichment technique from salt pan region. They were identified as <em>Bacillus

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Peng, Hui, Hua Yin, and Jin Shao Ye. "Alteration of Heavy Metal Fractions in Soil of Guiyu, South China by Native Bacillus cereus under the Effect of BDE209." Advanced Materials Research 726-731 (August 2013): 3901–7. http://dx.doi.org/10.4028/www.scientific.net/amr.726-731.3901.

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Microbiologic experiments together with Tessier sequential extraction and ICP-OES determination showed that distributions of Pb, Cu, Cd and Cr in soil of Guiyu, an e-waste dismantling region in South China, have changed after activities of native combined Bacillus cereus. Exchangeable fractions of these heavy metals were 1.52, 12.32, 3.98 and 34.50-fold those of original ones after 8 h interaction, here the organic phases were supposed to be the main alteration origin. The changing interaction time brought different impacts on concentration variation of heavy metals. Decabromodiphenyl ether (B

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Lemos, Ana Claudia Alcantara, Amanda Souza e. Souza, Pedro Henrique do Bomfim Nascimento, et al. "Bacillus cereus UFPEDA 1040B as a potential plant growth promoter: an in vitro study." Research, Society and Development 11, no. 12 (2022): e449111234517. http://dx.doi.org/10.33448/rsd-v11i12.34517.

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The Brazilian northeastern semi-arid is a region composed of countless microorganisms adapted to extreme conditions, and to ensure their survival, they can produce several biomolecules and develop peculiar mechanisms. The use of microorganisms has been reported as a promising alternative for promoting plant growth through the production of several bioactive molecules, which can replace chemical agents that cause environmental problems. In this study, Bacillus sp. Ar 16 (UFPEDA), isolated from the rhizosphere of Aroeira (Schinus terebinthifolia) from the Caatinga biome, northeastern semi-arid r

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Ahmed, Tablo Abdulrahim. "BACTERIOLOGICAL ISOLATION IN THE RESTAURANT OF HAWLER CITY KURDISTAN REGION, IRAQ." Science Heritage Journal 8, no. 2 (2024): 79–82. https://doi.org/10.26480/gws.02.2024.79.82.

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This investigation was applied to isolate and identify bacteria in three different restaurants order to predict their effects on people. Power plate method for isolation of bacteria the samples were inoculated into nutrient agar media by streak plate technique to obtain well isolated colonies. The results show the identity and the total colony forming units (CFU) for bacteria. The most frequently isolated bacteria were the bacterial species: Bacillus cereus, Clostridium botulinum, Escherichia coli, Salmonella typhi, Shigella dysenteriae, Staphylococcus aureus and Lactobacillus subtilus (11.12,

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Pomerantsev, Andrei P., Olga M. Pomerantseva, and Stephen H. Leppla. "A Spontaneous Translational Fusion of Bacillus cereus PlcR and PapR Activates Transcription of PlcR-Dependent Genes in Bacillus anthracis via Binding with a Specific Palindromic Sequence." Infection and Immunity 72, no. 10 (2004): 5814–23. http://dx.doi.org/10.1128/iai.72.10.5814-5823.2004.

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ABSTRACT Transformation of Bacillus anthracis with plasmid pUTE29-plcR-papR carrying the native Bacillus cereus plcR-papR gene cluster did not activate expression of B. anthracis hemolysin genes, even though these are expected to be responsive to activation by the global regulator PlcR. To further characterize the action of PlcR, we examined approximately 3,000 B. anthracis transformants containing pUTE29-plcR-papR and found a single hemolytic colony. The hemolytic strain contained a plasmid having a spontaneous plcR-papR intergenic region deletion. Transformation of the resulting plasmid pFP1

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Porrini, Constance, Cyprien Guérin, Seav-Ly Tran, Rozenn Dervyn, Pierre Nicolas, and Nalini Ramarao. "Implication of a Key Region of Six Bacillus cereus Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress." International Journal of Molecular Sciences 22, no. 10 (2021): 5079. http://dx.doi.org/10.3390/ijms22105079.

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Bacterial response to nitric oxide (NO) is of major importance for bacterial survival. NO stress is a main actor of the eukaryotic immune response and several pathogenic bacteria have developed means for detoxification and repair of the damages caused by NO. However, bacterial mechanisms of NO resistance by Gram-positive bacteria are poorly described. In the opportunistic foodborne pathogen Bacillus cereus, genome sequence analyses did not identify homologs to known NO reductases and transcriptional regulators, such as NsrR, which orchestrate the response to NO of other pathogenic or non-patho

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Wang, Hongbin, Yuanfu Zhang, Zhaoting Yuan, et al. "Crosslinking Mechanism on a Novel Bacillus cereus Transglutaminase-Mediated Conjugation of Food Proteins." Foods 11, no. 22 (2022): 3722. http://dx.doi.org/10.3390/foods11223722.

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Until now, Streptoverticillium mobaraense transglutaminase (TG) is the only commercialized TG, but limited information is known about its selection tendency on crosslinking sites at the protein level, restricting its application in the food industry. Here, four recombinant Bacillus TGs were stable in a broad range of pH (5.0–9.0) and temperatures (<50 °C), exhibiting their maximum activity at 50–60 °C and pH 6.0–7.0. Among them, TG of B. cereus (BCETG) demonstrated the maximal specific activity of 177 U/mg. A structural analysis indicated that the Ala147-Ala156 region in the substrate tunne

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Merghache, D., Z. Boucherit-Otmani, I. A. El Haci, I. Chikhi, and K. Boucherit. "Inhibitory Effect of Quercus ilex Wood Ash on the Growth of Pathogenic Microorganisms." Phytothérapie 16, S1 (2018): S269—S272. http://dx.doi.org/10.3166/phyto-2018-0071.

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This study was conducted to determine the antimicrobial (antibacterial and antifungal) activity of green oak (Quercus ilex) wood ash from Beni Snous region (Tlemcen, Algeria). For the evaluation of antimicrobial potency, we tested the effect of different ash concentrations on reference bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterobacter cloacae, and Proteus mirabilis) and reference yeasts (Candida albicans ATCC 10231 and Candida albicans 444IP). The antibacterial activity test

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Tinsley, Eowyn, Asma Naqvi, Agathe Bourgogne, Theresa M. Koehler, and Saleem A. Khan. "Isolation of a Minireplicon of the Virulence Plasmid pXO2 of Bacillus anthracis and Characterization of the Plasmid-Encoded RepS Replication Protein." Journal of Bacteriology 186, no. 9 (2004): 2717–23. http://dx.doi.org/10.1128/jb.186.9.2717-2723.2004.

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ABSTRACT A minireplicon of plasmid pXO2 of Bacillus anthracis was isolated by molecular cloning in Escherichia coli and shown to replicate in B. anthracis, Bacillus cereus, and Bacillus subtilis. The pXO2 replicon included (i) an open reading frame encoding the putative RepS replication initiation protein and (ii) the putative origin of replication. The RepS protein was expressed as a fusion with the maltose binding protein (MBP) at its amino-terminal end and purified by affinity chromatography. Electrophoretic mobility shift assays showed that the purified MBP-RepS protein bound specifically

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Nakano, Yoshio, Chiaki Kato, Eiichl Tanaka, Kinuko Kimura, and Koki Horikoshi. "Nucleotide Sequence of the Glutamine Synthetase Gene (glnA) and Its Upstream Region from Bacillus cereus." Journal of Biochemistry 106, no. 2 (1989): 209–15. http://dx.doi.org/10.1093/oxfordjournals.jbchem.a122834.

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Hafiz, Sharmin, and M. Habibur Rahman. "Detection and Diversity of Tetracycline Resistant Bacteria in Fish Ponds of Rajshahi Region." Journal of Bio-Science 32, no. 2 (2024): 23–30. http://dx.doi.org/10.3329/jbs.v32i2.75754.

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The growth of aquaculture as a key food production industry, particularly in Bangladesh, has led to the increased use of various chemicals and drugs. This study focuses on the critical analysis of bacterial profiles in the waters of fish farming ponds in the Rajshahi district of Bangladesh, a necessary step for disease management and addressing the public health issues related to antibiotic-resistant bacteria. The research found a significant presence of bacteria in these waters, with viable bacterial counts fluctuating from 3.0×10² to 4.2×10³ CFU/ml at a Tetracycline concentration of 32 µg/ml

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