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Journal articles on the topic 'Bacillus sphericus'

Author: Grafiati

Published: 4 June 2021

Last updated: 29 July 2025

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1

Mmuoegbulam, Augusta Oluchi, Augustine Agorye Unimke, Iniobong Ebenge Andy, Ogemdi Chinwendu Anika, and Rachael Teniola Macaulay. "Effect of variations in heterotrophic plate count methods on bacterial estimation in some sachet water in Calabar Metropolis." World Journal of Advanced Research and Reviews 1, no. 2 (2019): 018–27. https://doi.org/10.5281/zenodo.4308311.

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Heterotrophic plate count (HPC) or standard plate count is a standardized method for determining the density of aerobic and facultative anaerobic heterotrophic microorganisms in water. In this study, water samples were analyzed to ascertain the effects of temperature, media, and incubation time in HPC methods of bacterial estimation. The samples were analyzed using the membrane filtration and direct plating methods. No faecal coliform was detected in all the samples and at all temperature. However, some coliforms and heterotrophic bacteria were present in the water samples. The organisms were

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Joshi, Bindu, and Satish Walia. "Characterization by arbitrary primer polymerase chain reaction of polychlorinated biphenyl (PCB)-degrading strains of Comamonas testosteroni isolated from PCB-contaminated soil." Canadian Journal of Microbiology 41, no. 7 (1995): 612–19. http://dx.doi.org/10.1139/m95-081.

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In this study, we isolated and characterized biphenyl (BP) and polychlorinated biphenyl (PCB) degrading bacterial strains found in PCB-contaminated soil from an auto manufacturing plant located in Syracuse, New York. Twenty-one BP and PCB-degrading bacteria were randomly selected to form a representative sample of the bacterial population present at the site. Of the 21 bacteria, 13 were identified as Comamonas testosteroni, constituting about 60% of the bacterial population examined. Other PCB degraders identified were Acidovorax facilis, Alcaligenes xylosoxydans, Bacillus sphericus, Hydrogeno

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GC, Ganga, Kshama Parajuli, Ishan Gautam, Megha Raj Banjara, Prakash Ghimire, and Komal Raj Rijal. "Efficacy of Native Bacillus thuringiensis against Mosquito Vector." Journal of Nepal Health Research Council 21, no. 3 (2024): 479–85. http://dx.doi.org/10.33314/jnhrc.v21i3.4742.

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Background: Larval source management is an effective measure to control mosquito-borne diseases. Bacillus thuringiensis produces specific insecticidal crystal proteins toxic to mosquito larvae. In many parts of the South East Asian region, Bacillus thuringiensis is used for larval source management. In Nepal, larvicidal Bacillus thuringiensis is not available. The study aims to isolate larvicidal Bacillus thuringiensis from soil samples of Nepal to control mosquitoes.Methods: Native Bacillus thuringiensis was obtained from soil samples by the acetate selection method. It was identified by obse

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Yang, Yankun, Liwei Wang, Adelaida Gaviria, Zhiming Yuan, and Colin Berry. "Proteolytic Stability of Insecticidal Toxins Expressed in Recombinant Bacilli." Applied and Environmental Microbiology 73, no. 1 (2006): 218–25. http://dx.doi.org/10.1128/aem.01100-06.

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ABSTRACT The production of the vegetative mosquitocidal toxin Mtx1 from Bacillus sphaericus was redirected to the sporulation phase by replacement of its weak, native promoter with the strong sporulation promoter of the bin genes. Recombinant bacilli developed toxicity during early sporulation, but this declined rapidly in later stages, indicating the proteolytic instability of the toxin. Inhibition studies indicated the action of a serine proteinase, and similar degradation was also seen with the purified B. sphaericus enzyme sphericase. Following the identification of the initial cleavage si

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Hong, Sung Wook, Soon-Wo Kwon, Soo-Jin Kim, et al. "Bacillus oryzaecorticis sp. nov., a moderately halophilic bacterium isolated from rice husks." International Journal of Systematic and Evolutionary Microbiology 64, Pt_8 (2014): 2786–91. http://dx.doi.org/10.1099/ijs.0.058768-0.

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A Gram-stain-positive, aerobic, endospore-forming, moderately halophilic rod, designated strain R1T, was isolated from rice husks and subjected to a taxonomic study using a polyphasic approach. Strain R1T produced spherical or ellipsoidal endospores at a subterminal position in swollen sporangia, and was catalase- and oxidase-positive. The isolate grew optimally at 37 °C and pH 6.0–7.0, and could grow in the presence of up to 9 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R1T belongs to the genus Bacillus . The closest relatives of strain R1T were

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Reddy, A. J., D. V. S. R. Kumar, S. Rao, V. C. Prasannakumari, and V. Roja. "Endospore morphology and efficacy of indigenous Bacillus thuringiensis strains against okra fruit and shoot borer, Earias vittella." Journal of Environmental Biology 46, no. 2 (2025): 239–48. https://doi.org/10.22438/jeb/46/2/mrn-5294.

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Aim: The aim of this study was to determine the size of endospores of native Bacillus thuringiensis strains and identify an effective strain for managing the okra fruit and shoot borer, Earias vittella. Methodology: Six native Bacillus thuringiensis strains were cultured on T3 agar plates and incubated at 300C for 96 hrs. Subsequently, detailed examination of the endospores was conducted using a NanoSem 450 scanning electron microscope (SEM) to discern intrinsic differences between endospores from six isolates. Bioassays were performed using six native Bacillus thuringiensis cultures at variou

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Tampangallo, B. R., H. Anshary, S. Wulan, and R. Syah. "Characterization of Bacillus subtilis (BR 610) isolated from the intestines of Rabbitfish, Siganus guttatus, maintained in Floating Net at Awerange Bay, Barru." IOP Conference Series: Earth and Environmental Science 1273, no. 1 (2023): 012025. http://dx.doi.org/10.1088/1755-1315/1273/1/012025.

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Abstract The use of some species of Bacillus as a probiotic has been widely reported. In general, using probiotics in the exact isolation location will improve the performance of these probiotics. This study aims to characterize the Bacillus subtilis (BR610) isolate, successfully isolated from the intestines of Rabbitfish previously cultivated in the floating net of Awerange bay, Barru, South Sulawesi. The research includes morphological characterization of BR610 isolates, activity tests of amylase, cellulose, and proteinase enzyme. BR610 isolate inhibition test against several types of pathog

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Hieu, H. N., D. T. H. Trang, V. T. T. Hien, N. V. Nghia, N. T. Lam, and T. M. D. Nguyen. "Microorganism-mediated green synthesis of silver nanoparticles using Aspergillus niger and Bacillus megaterium." Digest Journal of Nanomaterials and Biostructures 17, no. 1 (2022): 359–67. http://dx.doi.org/10.15251/djnb.2022.171.359.

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Research in the field of nanotechnologies, the development of reliable and environmentally friendly processes for the synthesis of metallic nanoparticles constitutes an important element in this field. In this study, biosynthesis of silver nanoparticles using Bacillus megaterium and Aspergillus niger is reported. The results show that the characteristic UV-vis absorbance peak of AgNPs synthesized using Aspergillus niger was observed in the 433 - 448 nm range and around 475 nm with Bacillus megaterium. Most of the particles were spherical in shape and within a size range of 1 to 10.5 nm using A

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HOANG, Nhat Hieu, Van Nghia NGUYEN, Thi Tho NGUYEN, and Thi Mong Diep NGUYEN. "Impact of microwave synthesis time on the shape of silver nanostructures and their antibacterial activity." Journal of Metals, Materials and Minerals 33, no. 1 (2023): 101–6. http://dx.doi.org/10.55713/jmmm.v33i1.1631.

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Silver is a well-known effective antibacterial and disinfectant material with relatively few side effects. Nanosilver derived from it, have strong antibacterial, antifungal and broad-spectrum antiviral properties. This study describes how the microwave synthesis durations of silver nanoparticles affect their shape, and the effect of the shapes of these nanoparticles on their antibacterial activity. The optical properties of the nanosilver were examined through UV-Vis absorption spectroscopy. The morphology of the grain was determined by transmission electron microscopy (TEM), and the crystalli

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AL-MUDHAFFAR, EMAN M. A. AL-KHERSSANI AND ADNAN W. H. "Biosynthesis and Evaluation of the Qualitative Characteristics of Bacillus subtilis Silver Nanoparticles as Biopesticides." BIOPESTICIDES INTERNATIONAL 20, no. 01 (2024): 103. http://dx.doi.org/10.59467/bi.2024.20.103.

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Silver nanoparticles (AgNPs) were biosynthesized using Bacillus subtilis bacteria and characterized. First, the reaction color change from light to dark indicated the production of AgNPs. After that, the AgNPs were characterized by conducting a series of tests. UV/Vis spectrometer examination showed the highest value of the ultraviolet spectrum at 401 nm. The Fourier-transform infrared spectroscopy spectrometry examination showed primary and secondary amides from the solution, as evidenced by the presence of amino acid residues and proteins that strongly bind metals. When measuring X-ray diffr

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David, Alwin, and Ram Kumar. "Biogenesis of MnO₂ Nanoparticles Using Momordica Charantia Leaf Extract." ECS Transactions 107, no. 1 (2022): 747–59. http://dx.doi.org/10.1149/10701.0747ecst.

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In this present study, an easy, eco-friendly, and efficient method for the biogenesis of manganese dioxide nanoparticles (MnO2 NPs) using Momordica charantia leaf extract is discussed. The MnO2 NPs were synthesised by reduction of potassium permanganate using Momordica charantia leaf extract as a reducing agent. Fourier-transform infrared spectra exposed the contribution of the biomolecules in the Momordica charantia leaf extract for the formation of MnO2 NPs. The UV–visible spectrum of the biosynthesized MnO2 NPs displayed absorption peaks at 371 nm, which was the absorption maximum of MnO2 N

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Khan, Sehresh, Nazneen Akhtar, Shafiq Ur Rehman, Shaukat Shujah, Eui Shik Rha, and Muhammad Jamil. "Biosynthesized Iron Oxide Nanoparticles (Fe3O4 NPs) Mitigate Arsenic Toxicity in Rice Seedlings." Toxics 9, no. 1 (2020): 2. http://dx.doi.org/10.3390/toxics9010002.

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Arsenic (As) contamination has emerged as a serious public health concern worldwide because of its accumulation and mobility through the food chain. Therefore, the current study was planned to check the effect of Bacillus subtilis-synthesized iron oxide nano particles (Fe3O4 NP) on rice (Oryza Sativa L.) growth against arsenic stress (0, 5, 10 and 15 ppm). Iron oxide nanoparticles were extracellular synthesized from Bacillus subtilis with a desired shape and size. The formations of nanoparticles were differentiated through UV-Visible Spectroscopy, FTIR, XRD and SEM. The UV-Visible spectroscopy

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Seiler, Herbert, Mareike Wenning, and Siegfried Scherer. "Domibacillus robiginosus gen. nov., sp. nov., isolated from a pharmaceutical clean room." International Journal of Systematic and Evolutionary Microbiology 63, Pt_6 (2013): 2054–61. http://dx.doi.org/10.1099/ijs.0.044396-0.

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A novel red-pigmented bacterial strain, designated WS 4628T, was isolated from a pharmaceutical clean room of a vaccine-producing company and was investigated in a taxonomic study using a polyphasic approach. The strain was Gram-stain-positive, strictly aerobic, motile, catalase-positive and produced spherical to slightly ellipsoidal endospores in rods. The genomic DNA G+C content was 44.1 mol%. The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 and the predominant quinone was MK-6. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidenti

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Srivastava, Amrisha, Puneet Singh Chauhan, and Rachana Singh. "Characterization of Stress-Tolerant Bacteria for the Biosynthesis of Silver Nanoparticles and their Applications." Journal of Nano Research 68 (June 29, 2021): 70–80. http://dx.doi.org/10.4028/www.scientific.net/jnanor.68.70.

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The biogenesis of silver nanoparticles by microbes has become an essential branch in the field of nanotechnology because of its safe, environment-friendly, economical, and time-saving nature. In the current research work, we have screened some stress-tolerant bacteria based on pH, temperature, salt-tolerant efficacy and further utilized them for the synthesis of silver nanoparticles. The test bacterium was isolated from the soil sample through the serial dilution method on nutrient agar media (NAM). Based on identification using morphological characteristics, biochemical analysis, and 16srRNA

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Singh, Priyanka, Yeon Ju Kim, Hina Singh, Ramya Mathiyalagan, Chao Wang, and Deok Chun Yang. "Biosynthesis of Anisotropic Silver Nanoparticles byBhargavaea indicaand Their Synergistic Effect with Antibiotics against Pathogenic Microorganisms." Journal of Nanomaterials 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/234741.

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The strainBhargavaea indicaDC1 isolated from four-year-oldP. ginsengrhizospheric soil was used to perform rapid and extracellular biosynthesis of anisotropic silver nanoparticles. The ultraviolet-visible (UV-vis) spectra of the reaction mixture containing silver nanoparticles showed a peak at 460 nm, corresponding to the surface plasmon absorbance of silver nanoparticles. Field-emission transmission electron microscopy (FE-TEM) structural characterization revealed the nanobar, pentagon, spherical, icosahedron, hexagonal, truncated triangle, and triangular nanoparticles, with the size range fro

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Rodríguez-Prieto, Tamara, Philipp F. Popp, José Luis Copa-Patiño, et al. "Silver (I) N-Heterocyclic Carbenes Carbosilane Dendritic Systems and Their Imidazolium-Terminated Analogues as Antibacterial Agents: Study of Their Mode of Action." Pharmaceutics 12, no. 10 (2020): 968. http://dx.doi.org/10.3390/pharmaceutics12100968.

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Spherical dendrimers and dendrons containing silver(I) N-heterocyclic carbenes (Ag(I)-NHC) and additionally bow-tie metal-free dendritic systems were synthesized in a simple and straightforward synthetic procedure and subsequently characterized. The antibacterial activity was evaluated, and in parallel, a comparative study with the cationic analogue precursors was performed to explore the effect of silver ions in the dendritic structure. Other parameters, such as topology, generation, and hydrophobicity, of the imidazole substituents were also studied. All these dendritic systems presented ant

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Zheng, Aiping, Jun Zhu, Lingxia Wang, et al. "Characterization and expression of a novel holotype insecticidal crystal protein gene from nativeBacillus thuringiensisBM59-2." Canadian Journal of Microbiology 56, no. 2 (2010): 156–61. http://dx.doi.org/10.1139/w09-120.

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We characterized a novel holotype cry gene (cry52Ba1) harbored in a Bacillus thuringiensis isolate BM59-2 native to China that expresses a spherical crystal protein of about 80 kDa. In this study, the full length of the cry52Ba1 gene was cloned from this strain. Sequence analysis of the gene was also performed. Furthermore, cry52Ba1 was expressed in Escherichia coli BL21(DE)pLysS, and the resulting insecticidal activity showed that the Cry52Ba1 protein exhibited high larvicidal activity against Aedes aegypti (Diptera), with a lethal concentration 50 of 1.526 µg/mL (95% confidence: 0.740–3.499

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Wright, G., and H. J. Rogers. "Spherical and rod shaped protoplasts from Î²-lactam antibiotic treated cultures of Bacillus subtilis." FEMS Microbiology Letters 56, no. 1 (1988): 15–19. http://dx.doi.org/10.1111/j.1574-6968.1988.tb03143.x.

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Almog, Orna, Ana González, Daniela Klein, Harry M. Greenblatt, Sergei Braun, and Gil Shoham. "The 0.93Å Crystal Structure of Sphericase: A Calcium-loaded Serine Protease from Bacillus sphaericus." Journal of Molecular Biology 332, no. 5 (2003): 1071–82. http://dx.doi.org/10.1016/j.jmb.2003.07.011.

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Wright, G. "Spherical and rod shaped protoplasts from β-lactam antibiotic treated cultures of Bacillus subtilis". FEMS Microbiology Letters 56, № 1 (1988): 15–19. http://dx.doi.org/10.1016/0378-1097(88)90120-6.

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Tan, Lam Van, Thanh Tran, and Van Doan Thi. "Biosynthesis of Silver Nanoparticles from Bacillus licheniformis TT01 Isolated from Quail Manure Collected in Vietnam." Processes 9, no. 4 (2021): 584. http://dx.doi.org/10.3390/pr9040584.

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Silver nanoparticles (AgNPs) find a wide range of use in many fields, and the biosynthesis of AgNPs via biological routines has recently gained currency. In this study, Bacillus licheniformis TT01 strain was isolated from quail feces collected in Vietnam and evaluated for its ability to synthesize AgNPs. Through visual confirmation and ultraviolet and visible (UV–Vis) spectrum analysis, we found that the biosynthesis of AgNPs was realized in the process in which biomass of B. licheniformis TT01 was incubated with AgNO3 solution. Obtained AgNPs were then assayed for antibacterial activity again

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Wasano, Naoya, Hiroyuki Saitoh, Minoru Maeda, Akira Ohgushi, Eiichi Mizuki, and Michio Ohba. "Cloning and characterization of a novel genecry9Ec1encoding lepidopteran-specific parasporal inclusion protein from aBacillus thuringiensisserovargalleriaestrain." Canadian Journal of Microbiology 51, no. 11 (2005): 988–95. http://dx.doi.org/10.1139/w05-084.

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A novel δ-endotoxin gene from a lepidopteran-specific Bacillus thuringiensis serovar galleriae strain was cloned, and the full sequence of the cry gene was determined. The cloned 6.5-kb DNA fragment included the full sequence of the cry gene and three open reading frames located upstream of the cry gene. The gene, designated cry9Ec1, encodes a polypeptide of 1154 amino acid residues with a predicted molecular weight of 130 237. The deduced amino acid sequence of the Cry9Ec1 protein had the highest homology (77.7%) with the Cry9Ea1 protein when compared with existing Cry proteins. The expressio

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Battah, Moses Victor, Otitoju Olawale, Moses Abah, et al. "Microbial Isolates in Microplastic-Polluted Soil." African Journal of Biochemistry and Molecular Biology Research 1, no. 1 (2024): 693–705. https://doi.org/10.58578/ajbmbr.v1i1.3705.

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Microplastic pollution is a growing environmental concern, with plastic debris fragmenting into microscopic particles that contaminate soil ecosystems. Microplastics have become a major environmental concern due to their persistent presence in various ecosystems, including soil. They can act as pollutants and have detrimental effects on the environment and human health. This study aimed at isolating and identifying microbes (Bacteria and Fungi species) present in microplastic-polluted soil. Microplastic-polluted soil samples were collected from three sites (which were Kwararafa University Area

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Gill, Richard L., Jean-Philippe Castaing, Jen Hsin, et al. "Structural basis for the geometry-driven localization of a small protein." Proceedings of the National Academy of Sciences 112, no. 15 (2015): E1908—E1915. http://dx.doi.org/10.1073/pnas.1423868112.

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In bacteria, certain shape-sensing proteins localize to differently curved membranes. During sporulation in Bacillus subtilis, the only convex (positively curved) surface in the cell is the forespore, an approximately spherical internal organelle. Previously, we demonstrated that SpoVM localizes to the forespore by preferentially adsorbing onto slightly convex membranes. Here, we used NMR and molecular dynamics simulations of SpoVM and a localization mutant (SpoVMP9A) to reveal that SpoVM’s atypical amphipathic α-helix inserts deeply into the membrane and interacts extensively with acyl chains

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Oktavia, Ira, Aidah Nur Fithriah, Nur Umriani Permatasari, Enny Ratnaningsih, and Rukman Hertadi. "Levan Produced by the Halophilic Bacterium Bacillus licheniformis BK1 as a Nanoparticle for Protein Immobilization." Indonesian Journal of Chemistry 20, no. 3 (2020): 493. http://dx.doi.org/10.22146/ijc.41064.

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This study examined the potential of levan from the halophilic bacterium Bacillus licheniformis BK1 as a nanoparticle system for protein immobilization. Levan produced by B. licheniformis BK1 was obtained by incubating the bacterium in the optimized Belghith medium, containing 15% (w/v) sucrose, 7.5% (w/v) NaCl and pH 8, in a rotary shaker at 150 rpm for 16 h, at 40 °C. The structure of the levan produced was verified by FTIR and NMR. It appeared that the levan had the same structure as that from Erwinia herbicola. The obtained levan was then used as a nanoparticle system to immobilize BSA and

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Silva, Najara da, Ana Maria Guidelli Thuler, Irlan Leite de Abreu, Camila Chiaradia Davolos, Ricardo Antonio Polanczyk, and Manoel Victor Franco Lemos. "Characterization and selection of Bacillus thuringiensis isolates effective against Sitophilus oryzae." Scientia Agricola 67, no. 4 (2010): 472–78. http://dx.doi.org/10.1590/s0103-90162010000400015.

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The entomopathogenic bacterium Bacillus thuringiensis is a control agent with toxic and environmental characteristics that allows the control of pest insects according to the Integrate Pest Management (IPM) precepts. In order to find new strains, potentially toxic to Sitophilus oryzae L. 1763 (Coleoptera: Curculinidae), 1.073 strains of B. thuringiensis from parts of Brazil were used. Genetic material was extracted with InstaGene Matrix kit, used for the amplification of sequences in Polymerase chain reaction (PCR), and viewed in 1.5% agarose gel. The gene cry35Ba class was represented by 60 B

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K. Ajintha, J. Shifa Vanmathi, and M. Sithi Jameela. "Green Synthesis of Silver Nanoparticles from Phyllanthus emblica (Indian Gooseberry) for Potent Antimicrobial Efficacy." Journal of Advanced Zoology 44, no. 4 (2023): 914–20. http://dx.doi.org/10.17762/jaz.v44i4.2340.

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Nowadays cost-effective and environmentally friendly technologies for nanomaterial synthesis have gained attention in the biosynthesis of nanoparticles. The present study is focused on synthesizing silver nanoparticles using Phyllanthus emblica for a green approach. UV-Vis Spectrophotometry, XRD, SEM, and FTIR were used to characterise the synthesised silver nanoparticles. It was determined that spherical shaped nanoparticles by SEM examination, which revealed that the average particle size was 20 nm. Antibacterial activity of AgNPs analyzed with the help of Staphylococcus aureus, Vibrio chole

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John, Maria Sindhura, Joseph Amruthraj Nagoth, Kesava Priyan Ramasamy, et al. "Synthesis of Bioactive Silver Nanoparticles Using New Bacterial Strains from an Antarctic Consortium." Marine Drugs 20, no. 9 (2022): 558. http://dx.doi.org/10.3390/md20090558.

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In this study, we report on the synthesis of silver nanoparticles (AgNPs) achieved by using three bacterial strains Rhodococcus, Brevundimonas and Bacillus as reducing and capping agents, newly isolated from a consortium associated with the Antarctic marine ciliate Euplotes focardii. After incubation of these bacteria with a 1 mM solution of AgNO3 at 22 °C, AgNPs were synthesized within 24 h. Unlike Rhodococcus and Bacillus, the reduction of Ag+ from AgNO3 into Ag0 has never been reported for a Brevundimonas strain. The maximum absorbances of these AgNPs in the UV-Vis spectra were in the range

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Açıkgöz Erkaya, İlkay, Dilek Yalçın, and Belgin Erdem. "Eco-Friendly Synthesis of Silver Nanoparticles Using Klebsormidium subtile and Evaluation of their Antimicrobial, Anti-Quorum Sensing, and Antibiofilm Activities." Cumhuriyet Science Journal 45, no. 3 (2024): 454–64. http://dx.doi.org/10.17776/csj.1408553.

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In this study, both dry and fresh biomass extracts of Klebsormidium subtile were used for the synthesis of silver nanoparticles (AgNPs). The UV-visible spectrum showed an absorption peak at 430 nm, indicating the presence of AgNPs through surface plasmon resonance. FT-IR analysis identified bioactive functional groups, such as amines, which acted as stabilizing agents for the nanoparticles. SEM imaging revealed well-dispersed, spherical AgNPs ranging from 5 to 25 nm and 40 to 60 nm in size, accumulating on cell surfaces. EDS analysis confirmed the presence of elemental silver. The antimicrobia

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Kang, Beom Ryong, Joon Seong Park, Gwang Rok Ryu, Woo-Jin Jung, Jun-Seok Choi, and Hye-Min Shin. "Effect of Chitosan Coating for Efficient Encapsulation and Improved Stability under Loading Preparation and Storage Conditions of Bacillus Lipopeptides." Nanomaterials 12, no. 23 (2022): 4189. http://dx.doi.org/10.3390/nano12234189.

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This study aims to evaluate the effect of chitosan coating on the formation and properties of Bacillus cyclic lipopeptide (CLP)-loaded liposomes. A nanoencapsulation strategy for a chitosan-coated liposomal system using lecithin phospholipids for the entrapment of antibiotic CLP prepared from Bacillus subtilis KB21 was developed. The produced chitosan-coated CLP liposome had mean size in the range of 118.47–121.67 nm. Transmission electron microscopy showed the spherical-shaped vesicles. Fourier transform infrared spectroscopy findings indicated the successful coating of the produced CLP-loade

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Todorova, Maria, Ventsislava Petrova, Bogdan Ranguelov, et al. "Green Synthesis of Antimicrobial Silver Nanoparticles (AgNPs) from the Mucus of the Garden Snail Cornu aspersum." Molecules 30, no. 10 (2025): 2150. https://doi.org/10.3390/molecules30102150.

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The green synthesis of metal nanoparticles, mediated by extracts from various biological sources, leads to the formation of nanoparticles with unique characteristics and potential biomedical applications. In this study, a fraction of the mucus from the snail Cornu aspersum with MW > 20 kDa was used as a bioreducing and biostabilizing agent to obtain silver nanoparticles (AgNPs) in different pH media (pH 1.5, 3.5, and 7.0). The AgNPs were characterized using UV-visible spectroscopy, SEM, TEM, XRD, and FTIR. The synthesis at pH 1.5 and pH 3.5 in the presence of two reducing agents (i.e., the

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Tyagi, Radhika, Dr Meenakshi Kumari, and Dr Sukhvinder Singh. "Characterisation and synthesis of agnps from Aristolochia bracteolata leaves." International Journal of Multidisciplinary Research and Growth Evaluation 5, no. 5 (2024): 148–54. http://dx.doi.org/10.54660/.ijmrge.2024.5.5.148-154.

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Objective: The objective of this research was to present the antimicrobial potential of AgNP’s synthesised from Aristolochia bracteolata leaves extract. Methods: Evaluation of antimicrobial activity of the leaves extract was tested using agar well diffusion method along with the commercial control antibiotics (Ciprofloxacin) and the synthesised AgNPs were also tested against several microbial cultures (MTCC 10619, MTCC 0424, MTCC 872 & MTCC 1755). Further phytochemical analysis and characterisation of the synthesised nanoparticles was done to determine the shape and size of synthesised nan

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Nascimento, Tatiane Aparecida, Maria José Paes, Fernando Hercos Valicente, and Margareth Maria de Carvalho Queiroz. "Bioactive Potential of Some Bacillus thuringiensis Strains from Macapá, Amazon, Brazil, Against the Housefly Musca domestica (Diptera: Muscidae) Under Laboratory Conditions." Insects 16, no. 1 (2024): 27. https://doi.org/10.3390/insects16010027.

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The high pathogenic activity of Bacillus thuringiensis (Bt) strains against various insect orders has positioned it as the most effective, environmentally safe, and sustainable approach to integrated insect management. We aimed to identify Bt strains capable of effectively controlling the housefly Musca domestica, a species of significant medical, veterinary, and public health concern. Twelve Bt strains from Macapá, Brazil, were tested against housefly larvae. PCR was employed to detect genes encoding Cry and Cyt proteins and Vips. Six strains exhibited 70 to 100% larval mortality, with five c

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Umoren, Saviour A., Alexis M. Nzila, Saravanan Sankaran, Moses M. Solomon, and Peace S. Umoren. "Green synthesis, characterization and antibacterial activities of silver nanoparticles from strawberry fruit extract." Polish Journal of Chemical Technology 19, no. 4 (2017): 128–36. http://dx.doi.org/10.1515/pjct-2017-0079.

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Abstract Silver nanoparticles (AgNPs) have been synthesized in the presence of Strawberry fruit extract (SBFE) at room temperature. The synthesized AgNPs was characterized by UV-vis spectroscopy, SEM, EDS, XRD, TEM and FTIR. The UV-vis spectra of the AgNPs show SPR band at 450 nm. TEM results indicate that AgNPs are spherical in shape and size range between 7–65 nm. Antibacterial activity of the synthesized AgNPs has been assessed against Pseudomonas aeruginosa and Bacillus licheniformis. The results show that AgNPs exhibit inhibitory effect and effect is a function of AgNPs concentration. The

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C. Pomal, Nandan, Keyur D. Bhatt, and Dinesh S. Kundariya. "Antimicrobial Activity of Calix[4]pyrrole-entrenched Silver Nanoparticles and Its Application as Colorimetric and Spectrophotometric Sensing of L-Histidine." Oriental Journal Of Chemistry 39, no. 1 (2023): 30–39. http://dx.doi.org/10.13005/ojc/390104.

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A calix[4]pyrrole tetrahydrazide functionalized silver nanoparticles (CPTPH-AgNPs) coupled with colorimetric findings were prepared. In the variety of essential amino acids, it detected the L-Histidine exclusively with an LOD of 6.1 μM and LOQ of 18.5 μM. The CPTPH-AgNPs were exhibited the SPR spectrum at 408nm, which further characterized with TEM, EDEX and SAED in that they show the monodispersed spherical morphology of AgNPs with 13±2nm size. The antimicrobial potential of CPTPH-AgNPs towards Gram-positive bacterial-Staphylococcus aureus, Bacillus subtilis, Gram-negative bacteria-Escherichi

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Zhang, Li, Zhihong Xu, and Bharat K. C. Patel. "Bacillus decisifrondis sp. nov., isolated from soil underlying decaying leaf foliage." International Journal of Systematic and Evolutionary Microbiology 57, no. 5 (2007): 974–78. http://dx.doi.org/10.1099/ijs.0.64440-0.

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An aerobic bacterium, designated strain E5HC-32T, was isolated from soil underlying the decaying leaf litter of a slash pine forest located in south east Queensland, Australia. The strictly aerobic, motile rod-shaped cells (0.8–1.6×2.6–4.8 μm) produced subterminal spherical spores which distended the cells. Strain E5HC-32T grew optimally in 1 % trypticase soy broth (TSB) at 30 °C (temperature range for growth, 25–40 °C) and a pH of 8.4 (pH growth range, pH 7.1–9.1). Electron microscopic examination of negatively stained cells revealed the presence of peritrichous flagella and thin sections sho

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Baloch, Hadia, Aisha Siddiqua, Asif Nawaz, et al. "Synthesis and Characterization of Sulfur Nanoparticles of Citrus limon Extract Embedded in Nanohydrogel Formulation: In Vitro and In Vivo Studies." Gels 9, no. 4 (2023): 284. http://dx.doi.org/10.3390/gels9040284.

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The study aimed to synthesize non-noxious, clean, reliable, and green sulfur nanoparticles (SNPs) from Citrus limon leaves. The synthesized SNPs were used to analyze particle size, zeta potential, UV–visible spectroscopy, SEM, and ATR-FTIR. The prepared SNPs exhibited a globule size of 55.32 ± 2.15 nm, PDI value of 0.365 ± 0.06, and zeta potential of −12.32 ± 0.23 mV. The presence of SNPs was confirmed by UV–visible spectroscopy in the range of 290 nm. The SEM image showed that the particles were spherical with a size of 40 nm. The ATR-FTIR study showed no interaction, and all the major peaks

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Elamparithi, R., and K. Sujatha. "Influence of Organic Seaweed Pelleting on Seed Quality Parameters of Onion Seeds." International Journal of Environment and Climate Change 13, no. 11 (2023): 3349–55. http://dx.doi.org/10.9734/ijecc/2023/v13i113508.

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Aim: Small and irregular seed shapes make precision sowing difficult. Seed pelleting, which helps to make seed handling easier during sowing, is converting such seeds into bold, spherical shapes with a smooth surface. Seaweeds are a rich source of nutrients that promote growth, such as IAA, kinetin, zeatin, and gibberellins; auxins and cytokinins; metabolic enhancers; macro and micro elements; amino acids; and vitamins. Their use in crop plants has been shown to have positive effects, including early seed germination and establishment, improved crop performance and yield, increased resistance

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Monowar, Tahmina, Md Rahman, Subhash Bhore, Gunasunderi Raju, and Kathiresan Sathasivam. "Silver Nanoparticles Synthesized by Using the Endophytic Bacterium Pantoea ananatis are Promising Antimicrobial Agents against Multidrug Resistant Bacteria." Molecules 23, no. 12 (2018): 3220. http://dx.doi.org/10.3390/molecules23123220.

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Antibiotic resistance is one of the most important global problems currently confronting the world. Different biomedical applications of silver nanoparticles (AgNPs) have indicated them to be promising antimicrobial agents. In the present study, extracellular extract of an endophytic bacterium, Pantoea ananatis, was used for synthesis of AgNPs. The synthesized AgNPs were characterized by UV–Vis spectroscopy, FTIR, transmission electron microscopy (TEM), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), and Zeta potential. The antimicrobial potential of the AgNPs agai

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J., Thelma, and Balasubramanian C. "Ovicidal, larvicidal and pupicidal efficacy of silver nanoparticles synthesized by Bacillus marisflavi against the chosen mosquito species." PLOS ONE 16, no. 12 (2021): e0260253. http://dx.doi.org/10.1371/journal.pone.0260253.

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Microbial synthesis of silver nanoparticles is more advantageous and is eco-friendly to combat the various vectors that cause diseases in humans. Hence, in the present study a Bacillus strain is isolated from marine habitat and is evaluated for its ability to synthesize silver nanoparticles (AgNPs) and its efficacy evaluated against the immature stages of selected mosquito species. The effective candidate was confirmed to be Bacillus marisflavi after 16S rRNA sequencing. The synthesis of AgNPs was confirmed by UV-Vis spectrophotometer. Atomic Force Microscopic (AFM) analysis showed spherical n

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Mohammed, Eman Jassim, Ahmed E. M. Abdelaziz, Alsayed E. Mekky, et al. "Biomedical Promise of Aspergillus Flavus-Biosynthesized Selenium Nanoparticles: A Green Synthesis Approach to Antiviral, Anticancer, Anti-Biofilm, and Antibacterial Applications." Pharmaceuticals 17, no. 7 (2024): 915. http://dx.doi.org/10.3390/ph17070915.

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This study utilized Aspergillus flavus to produce selenium nanoparticles (Se-NPs) in an environmentally friendly and ecologically sustainable manner, targeting several medicinal applications. These biosynthesized Se-NPs were meticulously characterized using X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectroscopy, transmission electron microscope (TEM), and UV–visible spectroscopy (UV), revealing their spherical shape and size ranging between 28 and 78 nm. We conducted further testing of Se-NPs to evaluate their potential for biological applications, including antiviral, antica

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Wasano, N., K. H. Kim, and M. Ohba. "Delta-endotoxin proteins associated with spherical parasporal inclusions of the four Lepidoptera-specific Bacillus thuringiensis strains." Journal of Applied Microbiology 84, no. 4 (1998): 501–8. http://dx.doi.org/10.1046/j.1365-2672.1998.00371.x.

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Maslienko, L. V., L. A. Deynega, and E. A. Zaveryukha. "Mechanism of antagonist action of the promising bacterial strain 5B-1 of Bacillus subtilis on the sunflower dry rot pathogen Rhizopus oryzae Went & Prins. Geerl." Oil Crops 2, no. 202 (2025): 110–14. https://doi.org/10.25230/2412-608x-2025-2-202-110-114.

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Earlier in the laboratory of biomethod of V.S. Pustovoit All-Russian Research Institute of Oil Crops, as a result of gradual screening, a promising bacterial producer strain of microbiopreparation 5B-1 Bacillus subtilis, showing antagonism to the sunflower dry rot pathogen Rhizopus oryzae Went & Prins. Geerl. was isolated. In this study, the character and dynamics of changes in the pathogen mycelium during co-cultivation with the antagonist were studied using a light microscope. It was shown that the bacterial strain has antibiotic activity, which is expressed in the formation of spherical

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G.C., Ganga, Charu Arjya, Yamuna Khadka, and Sabina Dhamala. "Diversity of Insecticidal Crystal proteins (ICPs) of indigenous Bacillus thuringiensis strains." Tribhuvan University Journal of Microbiology 5 (September 26, 2018): 11–18. http://dx.doi.org/10.3126/tujm.v5i0.22296.

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Objectives: The purpose of this study was to characterize the indigenous Bacillus thuringiensis (Bt) isolated from the soil samples of central development region of Terai. Methods: A total of 50 soil samples collected from cultivated and barren fields of Terai region. Isolation was carried out using the acetate selection protocol as described by (Russell and Al 1987) with a slight modification. The Nutrient broth (NB) was acetated by using 0.25M sodium acetate which is a selective enrichment method for isolation of Bt. Characterization of the isolate was done by phenotyping methods (micr

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E., Bhuvaneswari, and Sagaya Giri R. "Synthesis and Evaluation of Antibacterial Activity of Silver Nanoparticles from Lantana Camara Leaf Extract." RESEARCH REVIEW International Journal of Multidisciplinary 3, no. 12 (2018): 397–401. https://doi.org/10.5281/zenodo.2483249.

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There were many work have been made based on the plant and its extract mediated synthesis of nanoparticles. Metal related nanoparticles are usually synthesized by wet chemical methods, where the chemicals used are quite often toxic and flammable. In this work, we describe a cost effective and environment friendly technique for green synthesis of silver nanoparticles from 1mM AgNO3 solution through the extract of Lantana camara leaf as it is acts as a reducing as well as capping agent. Nanoparticles were characterized using UV–Vis absorption spectroscopy and SEM analysis showed the averag

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Chintha, Muthaiah, Balaswamy Puligilla, and Sadanand Vajja. "Microwave Assisted in situ Coating of Silver Nanoparticles on Cotton Fabric using Kalanchoe delagoensis Leaf Extract and Screening of Biological Activity." Asian Journal of Chemistry 37, no. 5 (2025): 1044–48. https://doi.org/10.14233/ajchem.2025.33485.

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The application of nanoparticle coatings on cotton through microwave heating is a well-established technique that has garnered considerable interest as an innovative and promising approach. Silver nanoparticles (AgNPs) get significant attention owing to their remarkable physical and chemical characteristics. The nanoparticles on cotton capping with phytochemicals has enhanced their applicability in the medicinal field. This study focuses on the green synthesis of silver nanoparticles on cotton using Kalanchoe delagoensis (AgNPs@KD) under microwave. UV-visible, XRD and EDX spectral reports show

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Attathom, Tipvadee, Wiboon Chongrattanameteekul, Jariya Chanpaisang, and Ratchanee Siriyan. "Morphological diversity and toxicity of delta-endotoxin produced by various strains of Bacillus thuringiensis." Bulletin of Entomological Research 85, no. 2 (1995): 167–73. http://dx.doi.org/10.1017/s0007485300034234.

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AbstractLocal isolates of Bacillus thuringiensis were isolated from infected rice stemborers, soils, rice bran and rice mill dust. Of 83 isolates serotyped by B. thuringiensis H-antisera, 13 serotypes were identified. Those serotypes were assigned to B. thuringiensis of subspecies canadensis, galleriae, kenyae, kurstaki, neoleonensis, aizawai, alesti, entomocidus, tochigiensis and tolworthi. Three serotypes, 3a:3c:3d, 5a:5b/21 and 17/27, were unnamed and portend to be new subspecies. Morphological studies of delta-endotoxin or crystal protein of each subspecies showed significant differences i

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Zhuravliova, O. A., T. A. Voeikova, and V. G. Debabov. "Photocatalytic Activity of CdS Nanoparticles Obtained by Microbial Synthesis on the Model of Decolorization of Methylene Blue Dye." Biotekhnologiya 36, no. 6 (2020): 127–37. http://dx.doi.org/10.21519/0234-2758-2020-36-6-127-137.

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It has been demonstrated for the first time that biogenic nanoparticles of cadmium sulfide (NPsCdS) obtained using the bacterial strain Bacillus subtilis 168 by an optimized environmentally friendly, nature-like microbial synthesis are able to photocatalytically decolorize methylene blue dye under UV irradiation (365 nm). NPsCdS, characterized as quantum dots, fluoresce in the blue spectral region (400-440 nm), have a spherical shape, a diameter of 5±1 nm and a hydrodynamic diameter of 250--300 nm. The dynamics of methylene blue decolorization was studied depending on the NPsCdS and the dye co

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AboElmaaty, Sabah A., Ali A. Shati, Mohammad Y. Alfaifi, et al. "Biofilm Inhibitory Activity of Actinomycete-Synthesized AgNPs with Low Cytotoxic Effect: Experimental and In Silico Study." Microorganisms 11, no. 1 (2022): 102. http://dx.doi.org/10.3390/microorganisms11010102.

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The emergence of resistance by biofilm-forming bacteria has reached alarming and dangerous levels that threaten human civilization. The current study sought to investigate the antibiofilm potential of green-synthesized silver nanoparticles, mediated by a new Streptomyces strain. Zeta potential, transmission electron microscopy (TEM), and UV-Vis spectroscopy were used to analyze the biosynthesized AgNPs. Results revealed that silver nanoparticles had a size of (5.55 and 45.00 nm) nm and a spherical shape, with surface plasmon resonance (SPR) absorption at 400–460 nm in the UV-vis spectra establ

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Mahmoud, Ruaa Kamel. "The Use of Bio-Manufactured Nano-Zinc from Bacillus cereus in the Control of Dreshslera oryzae Fungus." IOP Conference Series: Earth and Environmental Science 1158, no. 7 (2023): 072027. http://dx.doi.org/10.1088/1755-1315/1158/7/072027.

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Abstract This research set out to create biosynthetic nano zinc ZnO-NPs by employing Bacillus cereus, characterize said nano zinc, and then test said nano zinc’s ability to thwart the spread of Dreshslera oryzae, the fungus responsible for rice’s brown spot disease. Bacillus cereus was used in a biological process to produce nano-sized zinc oxide, and this nano-sized zinc was then analyzed using a number of different methods. The ZnO-NPs generated from B. cereus had a nano zinc size of 22.48 nm, and their UV-Spectrophotometer examination indicated a significant absorption peak at 390 nm. Furth

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