Academic literature on the topic 'Bacillus subtilis metal bioremediation'
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Journal articles on the topic "Bacillus subtilis metal bioremediation"
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Prasannan, Dijo, and Chellaiah Arunkumar. "Pentafluorophenyl dipyrrin as probe for transition metal ion detection and bioremediation in Bacillus subtilis and Bacillus cereus." New Journal of Chemistry 41, no. 19 (2017): 11190–200. http://dx.doi.org/10.1039/c7nj02157g.
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Deborah, S., and J. Sebastin Raj. "Bioremediation of heavy metals from Distilleries Effluent using Microbes." Journal of Applied and Advanced Research 1, no. 2 (August 24, 2016): 23. http://dx.doi.org/10.21839/jaar.2016.v1i2.21.
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One of the major universal threats is water pollution. The untreated distilleries effluent discharge into the environment creates a serious problem to the living systems. It may contain carcinogenic aromatics amines, dyes, organic and inorganic chemical compounds. The physicochemical characterization of effluent was collected from Trichy Distilleries & Chemicals pvt Ltd, Tiruchirappalli, Tamilnadu, India and the chemical analyzed in Soil Testing Laboratory, Tiruchirappalli. The effluent was analyzed by different parameters. The physical parameters are BOD, COD, and DO, TDS, pH and electrical conductivity. Heavy metal analysis included Zinc, Lead, Manganese, Arsenic, Iron, Mercury, Copper, Chromium, Cadmium and Nickel. The soil sample was taken from Trichy Distilleries and Chemicals Ltd and analyzed by serial dilution technique. The three microbes such as Escherichia coli, Bacillus subtilis and Pseudomonas putida, were isolated from soil source. The isolated organism was treated with the effluent for various concentrations in various hours. The metal accumulations were identified in soil test lab, Trichy. The hyper accumulations of metal in microbes were identified. The present study explores the effectiveness of bioremediation has a simultaneous removal of Zinc, Lead, Manganese, Arsenic, Iron, Mercury, Copper, Chromium, Cadmium and Nickel from the distilleries effluent. Bacillus subtilis bacterial strains effectively remove the heavy metal from the distilleries effluent.
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Tanu, FZ, and S. Hoque. "Bacterial Tolerance to Heavy Metal Contents Present in Contaminated and Uncontaminated Soils." Bangladesh Journal of Microbiology 29, no. 2 (June 25, 2016): 56–61. http://dx.doi.org/10.3329/bjm.v29i2.28437.
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Present study dealt with identification of some heavy metal tolerant bacteria from contaminated industrial soils of Dhaka Export Processing Zone (DEPZ) at Savar, tannery area at Hazaribagh and uncontaminated agricultural soils of Dhamrai and Kushtia in Bangladesh and determination of their tolerance to chromium (Cr6+) and cadmium (Cd2+). A total of 15 isolates from four soil samples were provisionally identified as different species of Bacillus, Micrococcus and Pseudomonas based on their morphological, physiological, and biochemical characteristics. Among them eight colonies were separated based on high level of heavy metal tolerance and identified at molecular level by PCR technique and 16S rRNA gene sequencing as Micrococcus luteus strain P43 (E4), Bacillus pocheonensis strain TR2-6 (T6), Bacillus megaterium strain H2 (T8), Bacillus amyloliquefaciens strain SCSAAB0007 (D10), Bacillus cereus isolate PGBw4 (D11), Bacillus cereus strain ES-4a1 (K12), Bacillus subtilis strain 1320, (K13), and Bacillus subtilis strain DP14 (K14). The Maximum Tolerable Concentration (MTC) of bacterial strains to Cr6+ and Cd2+ ranged between 250-1250 ?g/ml and 30-150 ?g/ml, respectively in nutrient broth medium. From the metal tolerance investigation Bacillus was found as the most heavy metal tolerant to both Cr6+ and Cd2+ among the three genera. The identified heavy metal tolerant bacteria could be useful for the bioremediation of heavy metal contaminated environment.Bangladesh J Microbiol, Volume 29, Number 2, Dec 2012, pp 56-61
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Sizentcov, Aleksey, Elena Sal’nikova, Elena Barysheva, Yaroslav Sizentcov, and Veronika Sal’nikova. "Biotoxicity of heavy metal salts to Bacillus subtilis and their sorption properties." E3S Web of Conferences 157 (2020): 02012. http://dx.doi.org/10.1051/e3sconf/202015702012.
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The prospect of the use of microorganisms in medical and veterinary practice at the present stage of scientific development has high potential based on natural mechanisms of protection and adaptation of organisms to the effects of adverse factors. The biological adaptation potential of microorganisms to the effects of heavy metals found its practical application in studies of soil and water bioremediation. Based on the above, we set the aim to assess inhibitory characteristics of various heavy metal compounds, the extent of their impact on the growth of populations of probiotic strains Bacillus subtilis, and to study biosorption criteria of the strains. To achieve this aim, we used isolated cultures of probiotic strains B. subtilis 534 and B. subtilis 10641 extracted from drugs Sporobacterin and Vetom 1.1, respectively. Various chemical compounds of iron, copper, zinc, cadmium, and lead with a various anion component were used as growth inhibitors in the study. The criterion for the selection of compounds was a high level of their dissociation in water solutions. Data presented in this work experimentally confirm the capability of using probiotic strains in ecosystems of biological origin. A comparison of two strains of Bacillus subtilis showed a high level of resistance to xenobiotic elements with strain differences in the studied microorganism. The B. subtilis 534 strain revealed a higher level of resistance to compounds with a direct correlation between the inhibitory characteristics of the elements and the level of element sorption from the nutrient substrate.
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Imam, S. S. Ali. "Comparative Study of Heavy Metal Bioremediation in Soil by Bacillus Subtilis and Saccharomyces Cerevisiae." Indian Journal of Science and Technology 9, no. 1 (January 20, 2016): 1–7. http://dx.doi.org/10.17485/ijst/2016/v9i47/106911.
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Bhuiyan, Md Arifur Rahman, Tazeen Fatima Khan, Shahjahan Choudhury, and SM Imamul Huq. "Cadmium and lead tolerant bacteria isolated from industrial waste water." Dhaka University Journal of Biological Sciences 26, no. 1 (January 20, 2017): 29–38. http://dx.doi.org/10.3329/dujbs.v26i1.46347.
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An in vitro study was conducted to isolate, identify and characterize heavy metal resistant bacteria from industrial waste water and to determine their tolerance capacity to cadmium and lead. Different morphological, physiological and biochemical tests were carried out to identify the bacterial isolates in the waste water. A total of eight bacterial isolates viz., Staphyllococcus intermedius, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Escherichia coli, Acinetobacter baumanii, Pseudomonas flavescens and Acinetobacter lwofii were identified from metal polluted tannery and steel industrial areas. Bacterial response to cadmium tolerance was determined by treating them with CdCl2 solution at a rate of 1, 2, 2.5 and 5 μg/ml. In case of lead tolerant bacteria, PbCl2 solution was applied at a rate of 0.05, 0.125, 0.2 and 0.5 μg/ml. Pseudomonas aeruginosa isolated from waste water of steel industries and Bacillus cereus from tannery area was found to be the most tolerant species to the different doses of cadmium and lead. The study indicated that Pseudomonas aeruginosa and Bacillus cereus could be good candidates for the treatment and elimination of heavy metals from industrial waste water. The present study may be helpful to the bioremediation of heavy metals in the contaminated environment.
Dhaka Univ. J. Biol. Sci. 26(1): 29-38, 2017 (January)
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Mardiyono, Mardiyono, Sajidan Sajidan, Mohammad Masykuri, and Prabang Setyono. "Bioremediation Using Bacillus subtilis and Saccharomyces cerevisiae to Reduce Chromium in Electroplating Liquid Waste." SAINS TANAH - Journal of Soil Science and Agroclimatology 16, no. 2 (December 30, 2019): 191. http://dx.doi.org/10.20961/stjssa.v16i2.29730.
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<p>The electroplating industry produces liquid waste containing a small number of heavy metals but is toxic. Wastewater containing chromium (Cr) absorbed into the soil will affect soil fertility. Waste management is needed so that the abiotic and biotic environment is not poisoned by Cr. Bioremediation using bacterial and fungal microbes are applicable to reduce Cr levels in electroplating liquid waste. The purpose of this research was to investigate the reduction level of Cr in electroplating liquid waste through bioremediation using <em>Bacillus subtilis</em> and <em>Saccharomyces cerevisiae</em>. Laboratory experiments were conducted using variations in microbial concentrations (10<sup>2.5 </sup>cells ml<sup>-1</sup> and 10<sup>5</sup> cells ml<sup>-1</sup>), variations in microbial types (<em>Bacillus subtilis</em> bacteria, <em>Saccharomyces cerevisiae</em> fungi, and mixtures of both microbes), and variations in incubation time (6, 12, and 24 hours). The initial Cr concentration and the results of the bioremediation process were determined by measuring the absorbance and the Cr levels using Atomic Absorption Spectrophotometry (AAS). Based on experiments, the use of <em>Bacillus subtilis </em>10<sup>2.5</sup> cells ml<sup>-1</sup> with a 24-hour incubation time reach the highest percentage reduction in Cr (88.96%), followed by 12-hours incubation time (84.73%), and 6-hours incubation time (79.21%). Furthermore, the use of a microbial mixture of <em>Bacillus subtilis</em> and <em>Saccharomyces cerevisiae</em> 10<sup>2.5</sup> cells ml<sup>-1</sup> with 6-hours, 12-hours and 24-hours incubation time was able to reduce the levels of Cr respectively by 77.46%; 80.18% and 83.04%. Next, <em>Saccharomyces cerevisiae</em> 10<sup>5</sup> cells ml<sup>-1</sup> with 6-hours, 12-hours, and 24-hours incubation time was able to reduce levels of Cr in a row by 50.17%; 52.35% and 55.63%. The results of this study indicate that the bioremediation process using the microbial <em>Bacillus subtilis</em> and <em>Saccharomyces cerevisiae</em> is proven to reduce the levels of Cr in the electroplating industry wastewater. The highest reduction results were achieved on the use of 24-hour incubation time and the use of <em>Bacillus subtilis</em> with a concentration of 10<sup>2.5</sup> cells ml<sup>-1</sup> at 88.96%.</p>
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K, Suchitra. "Reclamation of Abandoned Mine Soil Using Biosurfactant." International Journal for Research in Applied Science and Engineering Technology 9, no. 9 (September 30, 2021): 23–28. http://dx.doi.org/10.22214/ijraset.2021.37395.
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Abstract: Mining results in enormous land damage, varying the ecosystem of microbial culture and disturbing plant life leading to annihilation of the land. Due to this researcher’s has shifted their focus to alternative methods for the sustainable development. The present study aims at environmental friendly and cost effective technique for the reclamation of abandoned mine soil using bio-surfactant. In the current investigation bio-surfactant was produced using Bacillus Subtilis MTCC no. 1427 on 20% spent wash collected from distillery unit. The mined soil with bio-surfactant was able to minimize heavy metal concentration and the plants grown on this soil were healthy similar to the results of the agricultural soil. Keywords: Mining, Abandoned, Reclamation, Heavy Metals, Re-vegetation, Bioremediation.
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Xiao, Xian, Yan Zhu, Yuexiang Gao, Jing Fu, Yuan Zhao, and Lihua Zhao. "Inoculation of paddy soils with Rhodopseudomonas palustris enhanced heavy metal immobilisation." Plant, Soil and Environment 67, No. 1 (January 11, 2021): 55–60. http://dx.doi.org/10.17221/355/2020-pse.
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To investigate the effect of microbial inoculum on soil heavy metal immobilisation, pot experiments were conducted with paddy soils contaminated by cadmium (Cd), lead (Pb), arsenic (As), and mercury (Hg), respectively. The results showed that the inoculation of Rhodopseudomonas palustris was more effective in the immobilisation of Pb and Cd in soils than the composite of R. palustris and Bacillus subtilis. Interestingly, a lower dosage of inoculum immobilised significantly more heavy metals than the higher dosage, potentially due to the competition of bacteria with limited nutrients. The heavy metal contents in rice grains also supported this finding, as less Pb and Cd were accumulated under the lower dosage. However, there were limited effects of microbial inoculations on the immobilisation of Hg and As. In general, our study indicated the effectiveness of R. palustris in immobilising Pb and Cd in soils and highlighted the importance of determining the optimal dosage of inoculum in bioremediation.
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Xiao, Xian, Yan Zhu, Yuexiang Gao, Jing Fu, Yuan Zhao, and Lihua Zhao. "Inoculation of paddy soils with Rhodopseudomonas palustris enhanced heavy metal immobilisation." Plant, Soil and Environment 67, No. 1 (January 11, 2021): 55–60. http://dx.doi.org/10.17221/355/2020-pse.
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To investigate the effect of microbial inoculum on soil heavy metal immobilisation, pot experiments were conducted with paddy soils contaminated by cadmium (Cd), lead (Pb), arsenic (As), and mercury (Hg), respectively. The results showed that the inoculation of Rhodopseudomonas palustris was more effective in the immobilisation of Pb and Cd in soils than the composite of R. palustris and Bacillus subtilis. Interestingly, a lower dosage of inoculum immobilised significantly more heavy metals than the higher dosage, potentially due to the competition of bacteria with limited nutrients. The heavy metal contents in rice grains also supported this finding, as less Pb and Cd were accumulated under the lower dosage. However, there were limited effects of microbial inoculations on the immobilisation of Hg and As. In general, our study indicated the effectiveness of R. palustris in immobilising Pb and Cd in soils and highlighted the importance of determining the optimal dosage of inoculum in bioremediation.
Dissertations / Theses on the topic "Bacillus subtilis metal bioremediation"
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Singleton, Chloe. "Metal binding studies of CopZ and CopA from Bacillus subtilis." Thesis, University of East Anglia, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.439937.
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Montagnolli, Renato Nallin [UNESP]. "Biodegradação de derivados do petróleo com a aplicação de biossurfactante produzido por Bacillus subtilis." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/94963.
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A biodegradação tem sido há muito tempo um dos principais campos de pesquisa em microbiologia, cuja utilidade inclui a biodegradação de substâncias orgânicas e inorgânicas. Microrganismos podem ser ótimas ferramentas de remoção de poluentes em solo, água e sedimentos pelas suas inúmeras vantagens sobre outros processos. Para a limpeza de ambientes com hidrocarbonetos pela biorremediação deve-se explorar a habilidade dos microrganismos e compreender a maneira pela qual o processo ocorre em diferentes derivados do petróleo. Existem estudos que propõem a biorremediação de ambientes contaminados por hidrocarbonetos pela inoculação de microrganismos com potencial biodegradador e a adição de biossurfactantes. O presente trabalho estudou a biodegradação de diferentes derivados do petróleo com a proposta de aprimorar o processo pelo uso de biossurfactante produzido por Bacillus subtilis. Também foi avaliada a toxicidade das substâncias antes e depois da biodegradação. Os biossurfactantes produzidos foram adicionados aos ensaios de biodegradação de diferentes tipos de derivados do petróleo, incluindo óleo cru, compostos fenólicos, querosene, gasolina e óleos lubrificantes automotivos sintéticos e usados. Foram utilizados também, como comparação, óleos vegetais de soja e biodiesel. O estudo foi desenvolvido utilizando a técnica de respirometria a qual monitora a formação de CO2. Também foi utilizada a técnica de colorimetria pela análise da coloração do indicador redox DCPIP. Assim, a partir dos dados obtidos foram observados os diferentes perfis de biodegradação das diferentes substâncias, a fim de obter uma descrição de como a aplicação de biossurfactante produzido por B. subtilis afeta a biodegradação dos derivados do petróleo. Observou-se que a biodegradação dos derivados do petróleo ocorreu pela ação de consórcio de microrganismos...
Biodegradation has been for a long time a main field of study in microbiology whose application includes organic and inorganic substances biodegradation. Microorganisms can be great pollutant removal tools in soil, water and sediments due to their advantages compared to other processes. In order to accomplish hydrocarbon-polluted environments cleanup by bioremediation, the microorganisms’ capabilities must be exploited and studied, so then information on different petroleum byproducts can be achieved. Some studies propose environmental biorremediation not only by hydrocarbon-degrading microorganisms’ insertion, but also by adding biossurfactants. In this work, different petroleum byproducts biodegradation was studied, aiming a biodegradation improvement through inclusion of a Bacillus subtilis produced biosurfactant. Biosurfactant production was also evaluated, as well as the substances toxicity before and after biodegradation. The biossurfactant produced was added to biodegradation assays of many different substances: crude oil, phenolic compounds, kerosene, gasoline and automotive lubricant oils. As a comparison, vegetable soy oils and biodiesel had their biodegradation performance evaluated. This study was developed by using the respirometric technique which is capable of evaluate CO2 production during biodegradation. Also, another biodegradation monitoring technique was used with a redox dye and colorimetry. Thus, from data obtained, different biodegradation profiles were estabilished and a precise description on how the biossurfactant affects petroleum byproducts biodegradation could be designed. It was observed that biodegradation occurred through microorganism consortium and B. subtilis. Also, the biossurfactant application yielded a better biodegradation. The mathematical modeling of the biodegradation process was also possible... (Complete abstract click electronic access below)
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Montagnolli, Renato Nallin. "Biodegradação de derivados do petróleo com a aplicação de biossurfactante produzido por Bacillus subtilis /." Rio Claro : [s.n.], 2011. http://hdl.handle.net/11449/94963.
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Orientador: Edério Dino BidóiaBanca: Edson Aparecido Abdul Nour
Banca: Maria Aparecida Marin Morales
Resumo: A biodegradação tem sido há muito tempo um dos principais campos de pesquisa em microbiologia, cuja utilidade inclui a biodegradação de substâncias orgânicas e inorgânicas. Microrganismos podem ser ótimas ferramentas de remoção de poluentes em solo, água e sedimentos pelas suas inúmeras vantagens sobre outros processos. Para a limpeza de ambientes com hidrocarbonetos pela biorremediação deve-se explorar a habilidade dos microrganismos e compreender a maneira pela qual o processo ocorre em diferentes derivados do petróleo. Existem estudos que propõem a biorremediação de ambientes contaminados por hidrocarbonetos pela inoculação de microrganismos com potencial biodegradador e a adição de biossurfactantes. O presente trabalho estudou a biodegradação de diferentes derivados do petróleo com a proposta de aprimorar o processo pelo uso de biossurfactante produzido por Bacillus subtilis. Também foi avaliada a toxicidade das substâncias antes e depois da biodegradação. Os biossurfactantes produzidos foram adicionados aos ensaios de biodegradação de diferentes tipos de derivados do petróleo, incluindo óleo cru, compostos fenólicos, querosene, gasolina e óleos lubrificantes automotivos sintéticos e usados. Foram utilizados também, como comparação, óleos vegetais de soja e biodiesel. O estudo foi desenvolvido utilizando a técnica de respirometria a qual monitora a formação de CO2. Também foi utilizada a técnica de colorimetria pela análise da coloração do indicador redox DCPIP. Assim, a partir dos dados obtidos foram observados os diferentes perfis de biodegradação das diferentes substâncias, a fim de obter uma descrição de como a aplicação de biossurfactante produzido por B. subtilis afeta a biodegradação dos derivados do petróleo. Observou-se que a biodegradação dos derivados do petróleo ocorreu pela ação de consórcio de microrganismos... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Biodegradation has been for a long time a main field of study in microbiology whose application includes organic and inorganic substances biodegradation. Microorganisms can be great pollutant removal tools in soil, water and sediments due to their advantages compared to other processes. In order to accomplish hydrocarbon-polluted environments cleanup by bioremediation, the microorganisms' capabilities must be exploited and studied, so then information on different petroleum byproducts can be achieved. Some studies propose environmental biorremediation not only by hydrocarbon-degrading microorganisms' insertion, but also by adding biossurfactants. In this work, different petroleum byproducts biodegradation was studied, aiming a biodegradation improvement through inclusion of a Bacillus subtilis produced biosurfactant. Biosurfactant production was also evaluated, as well as the substances toxicity before and after biodegradation. The biossurfactant produced was added to biodegradation assays of many different substances: crude oil, phenolic compounds, kerosene, gasoline and automotive lubricant oils. As a comparison, vegetable soy oils and biodiesel had their biodegradation performance evaluated. This study was developed by using the respirometric technique which is capable of evaluate CO2 production during biodegradation. Also, another biodegradation monitoring technique was used with a redox dye and colorimetry. Thus, from data obtained, different biodegradation profiles were estabilished and a precise description on how the biossurfactant affects petroleum byproducts biodegradation could be designed. It was observed that biodegradation occurred through microorganism consortium and B. subtilis. Also, the biossurfactant application yielded a better biodegradation. The mathematical modeling of the biodegradation process was also possible... (Complete abstract click electronic access below)
Mestre
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Randazzo, Paola. "Study of the regulatory network linked to metal ion homeostasis in Bacillus subtilis." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS387.
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Le projet de thèse concerne l’étude des réseaux de régulation en lien avec l’homéostasie des ions métalliques chez la bactérie à Gram-positif Bacillus subtilis. Les ions métalliques tels que Fe(II), Mn(II) et Zn(II) sont essentiels dans un grand nombre de processus cellulaires entant que cofacteur d’enzymes ou en tant qu’élément structural dans les protéines. Cependant, à trop hautes concentrations, ils peuvent avoir des effets toxiques en endommageant la membrane cellulaire et l’ADN ainsi qu’en inactivant la fonction de certaines protéines. De plus, en condition aérobie, B. subtilis produit du peroxyde d’hydrogène H₂O₂ qui réagit avec les ions Fe(II) pour produire des radicaux libres hautement toxiques pour la cellule. La régulation de l’homéostasie des ions métalliques doit donc être parfaitement régulée et coordonnée avec les autres processus cellulaires. J’essaie de comprendre de façon globale, via des approches expérimentales à grande échelle, les interconnexions entre les régulateurs de l’homéostasie des ions métalliques et autres voies métabolique dans Bacillus subtilisThe present doctoral thesis concerns the study of the regulatory network linked tometal ions homeostasis in the Gram+ Bacillus subtilis. Metal ions such as Fe(II), Mn(II) andZn(II) are essential for many metabolic processes, since they function as enzyme cofactors andstructural ligands of proteins. Changes in ions availability can alter activity of enzymes of thecarbon metabolism and lead to changes in gene expression. In addition, the modulation of metalion homeostasis is intimately linked with the oxidative stress response: during aerobic growth,hydrogen peroxide is generated and it rapidly reacts with ferrous iron to form ROS molecules.Hence, regulation of metal ions uptake/efflux has to be finely regulated and coordinated with othercellular processes. With the present project, I aim to understand at system’s level how Bacillussubtilis integrates the control of metal ions homeostasis with other metabolic processes
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Nguyen, Sy Le Thanh, Keitarou Kimura, Thi Tuyen Do, and Thi Ngoc Anh Le. "Isolation, characterization of Bacillus sp. producing heavy metal absorption γ-PGA." Technische Universität Dresden, 2018. https://tud.qucosa.de/id/qucosa%3A32710.
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Poly-gamma-glutamic acid (γPGA), which is a biodegradable, non-immunogenic and unusual anionic amino-acid polymer consist of D- and L-glutamic acid units, was exploited for a wide array of useful applications. Bacillus are well known cellular system important for fermentation to synthesize γPGA, which is used as thickener, drugs carrier, cryoprotectant, humectant, biological adhesive, flocculants, or heavy metal absorbent. This study focused on the isolation of Bacillus spp. that is possible to produce γ-PGA from different soil samples from different places in Vietnam. Study the effect of precursors, temperature, carbon sources, times and pH on γ-PGA production. From 31 soil samples and 4 straws samples, strain 20.2 which produced the highest γ-PGA yields (riches 15.2 mg/ml), was identified as Bacillus sp. 20.2 by molecular biology method. The suitable conditions for growing of Bacillus sp. 20.2 strain to produce γ-PGA are at 37°C, pH 7 after 72 hours. Citric acid instead of glucose in a GSP medium is better for producing γ-PGA by strain Bacillus sp. 20.2.Poly-gamma-glutamic acid (γ-PGA) là một polymer amino-acid gồm D và L-glutamic acid, có khả năng phân hủy sinh học, không gây miễn dịch, đã được ứng dụng rộng rãi trong công nghiệp, y học. Bacillus subtilis được biết đến là hệ thống tế bào ý nghĩa quan trọng trong quá trình lên men để tổng hợp γ-PGA. γ-PGA hòa tan trong nước, phân hủy sinh học và không độc đối với con người và môi trường. γ-PGA ổn định với nhiều protease vì các protease thường không nhận acid γ- glutamic (Obst et al., 2004). γ-PGA có cấu trúc đồng phân đơn giản, không gây miễn dịch. Do đó, γ-PGA đã được quan tâm ứng dụng trong các lĩnh vực như y học, công nghiệp thực phẩm, mỹ phẩm và đặc biệt là xử lý nước nhiễm kim loại nặng. Trong nghiên cứu này chúng tôi tập trung phân lập, tuyển chọn các chủng Bacillus có khả năng sinh tổng hợp PGA cao. Sau đó định danh và đánh giá khả năng sinh tổng hợp PGA từ chủng đã phân lập được. Kết quả cho thấy từ 34 mẫu rơm và đất, chúng tôi đã phân lập được chủng với mã số 20.2 có khả năng sinh PGA cao nhất đạt 15.2 mg/ml. Chủng này đã được định danh bằng phân tích trình tự gene 16S rRNA và thuộc loài Bacillus sp. Môi trường thích hợp sinh tổng hợp PGA là GSP ở điều kiện 37oC pH7 sau 72 giờ nuôi cấy.
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Clark, Amy Louise. "Mechanisms of metal binding and resistance to toxic metals in bacteria from soils polluted with toxic metals." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341456.
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Gonzaga, Lívia Vieira. "Surfactina desidratada como agente potencializador da biorremediação de solos contaminados com petróleo." Universidade Federal de Viçosa, 2013. http://locus.ufv.br/handle/123456789/5369.
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Previous issue date: 2013-12-13Conselho Nacional de Desenvolvimento Científico e Tecnológico
The effectiveness of spray dried surfactin, produced from a culture of Bacillus subtilis 4914 strain, was evaluated in microcosms containing sandy clay loam soil (SCL) or clayey soil (CLA) contaminated with petroleum. The highest respiratory rates in the microcosms additioned of petroleum demonstrated that oil was utilized as carbon source for microbiota in the microcosms of both soils. Maltodextrin, utilized as a drying adjuvants in the preparation of spray dried surfactin, was also utilized as a source of carbon and energy. The means of accumulated CO2 during the decline phase of the respiration rate shared by all microcosms of a same soil type, ranged from 47,6 to 137,7 μmol g-1 for SCL soil and from 37,5 to 98,5 μmol g-1 for CLA soil. The removal of TPH ranged from 19,6 to 38,4 % and from 62,6 to 73,2 % for the SCL and CLA soils, respectively. Surfactin in its two forms (in liquid solution and dried) influenced positively the degradation of the TPH in the SCL and CLA soils and in late phase of bioremediation. Maltodextrin did not interfere in the degradation of TPH. The lowest values of residual TPH in microcosms assembled with CLA soil were attributed to the highest microbial activity and biomass. The microbial community dynamics was studied by T-RFLP multiplex analysis of the groups Bacteria, Fungi e Archaea. The results demonstrated the occurrence of negative impact of oil on the microbial communities of all microcosms of both soil types, in the beginning of incubation period, and recovery of the community structure in subsequent times. The fungal community showed highest values of Shannon-Weaver index (H ), Simpson index (D) and Richness (S), followed by the Bacteria. In conclusion, spray dried surfactin favors the biodegradation of petroleum hydrocarbons in soils.
A efetividade da surfactina desidratada em spray dryer, produzida a partir do cultivo do isolado Bacillus subtilis LBBMA 4914, foi avaliada em microcosmos contendo amostras de solos franco-argilo-arenoso (FAA) ou argiloso (ARG) contaminados com petróleo. As taxas respiratórias mais elevadas nos microcosmos adicionados de petróleo demonstraram que o petróleo foi utilizado como fonte de carbono pelas microbiotas nos microcosmos de ambos os tipos de solo. A maltodextrina, utilizada como adjuvante de secagem na preparação da surfactina desidratada por spray dryer, foi também utilizada como fonte de carbono e energia. As médias dos valores de CO2 acumulado durante a fase de declínio das taxas respiratórias compartilhada por todos os microcosmos em um mesmo tipo de solo variaram entre 47,6 a 137,7 μmol g-1 (microcosmos com o solo FAA) e 37,5 a 98,5 μmol g-1 (microcosmos com solo ARG). A remoção dos HTPs variou entre 19,6 a 38,4 % (solo FAA) e 62,6 a 73,2 % (solo ARG). A surfactina em suas duas formas (solução e desidratada) influenciou positivamente a degradação dos HTPs nos solos FAA e ARG e em fase tardia da biorremediação. A maltodextrina não interferiu na degradação dos HTPs. Os menores valores de HTPs residuais em microcosmos montados com o solo ARG foram atribuídos às maiores atividade e biomassa microbianas. A dinâmica das comunidades microbianas foi estudada pela análise de T-RFLP multiplex dos grupos de Bacteria, Fungi e Archaea. Os resultados demonstraram a ocorrência de impacto negativo do petróleo nas comunidades microbianas de todos os microcosmos em ambos os tipos de solo, no início do período de incubação, e recuperação da estrutura das comunidades nos tempos subsequentes. A comunidade de fungos apresentou valores mais elevados dos índices de Shannon-Weaver (H ), Simpson (D) e Riqueza (S), seguida pela de Bacteria. Conclui-se que surfactina desidratada por spray-drying favorece a biodegradação de hidrocarbonetos de petróleo em solos.
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Santos, Carla Roberta da Silva Neves dos. "Biodegradação de benzeno por Bacillus subtilis em águas de rejeitos industriais e de osmose reversa; validação do sistema com grãos de kefir e Kalanchoe pinnata como indicadores biológicos (OU) Bioprocessos e bioindicadores de tratamento e monitoramento de águas: desenvolvimento e aplicações." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/9/9135/tde-22122016-171405/.
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Atualmente, a má qualidade da água continua a representar uma grande ameaça para a saúde humana, causando doenças graves, como diarreias, e diversos tipos de câncer devido a ingestão de compostos aromáticos e metais pesados. Estima-se que 88% dos casos dessas doenças sejam atribuídos ao abastecimento de água inseguro, saneamento e higiene. Este fato, em grande parte, deve-se ao mal controle de qualidade das águas tratadas. Assim, atualmente, uma das principais preocupações no estabelecimento de índices de qualidade de água, seja em base biológica ou através de métodos físico-químicos e matemáticos, é tornar as avaliações ecológicas com fins de rotina aplicáveis para pessoal não especializado. O presente trabalho, visando oferecer à sociedade subsídios para a adoção de tecnologias alternativas de tratamento e monitoramento de qualidade de águas, realizou uma análise sobre os potenciais dos Grãos de kefir e da planta kalanchoe pinnata como elementos bioindicadores de qualidade de águas tratadas, por meio de biorremediação com o micro-organismo Bacillus subtilis, e águas tratadas na Unidade de beneficiamento de águas (UPIBA). Os resultados dos experimentos evidenciaram que os grãos de kefir geraram respostas biológicas distintas, quando submetidos a diferentes concentrações de benzeno, presente em águas biorremediadas com o B. subtilis. E os ensaios aplicando a K. pinnata como agente bioindicador de qualidade de águas pluviais e SABESP, sugeriram que a mesma pode ser uma excelente bioindicadora em águas eutrofizadas, isto é, ricas em matéria orgânicaCurrently, poor quality water continues to pose a major threat to human health, causing serious illnesses such as diarrhea and various types of cancer due to the ingestion of aromatic compounds and heavy metals. It is estimated that 88% of these diseases\' cases are caused by unsafe water supply, sanitation and hygiene. This is largely due to poor quality control of treated water. Thus, currently, one of the major concerns in establishing water quality indicators, whether on a biological basis or through physical-chemical and mathematical methods, is to make routine ecological assessments applicable to non-specialized personnel. The present paper, aiming to offer society subsidies for the adoption of alternative technologies for treating and monitoring water quality, carried out an analysis on the potentials of kefir grains and the kalanchoe pinnata plant as bioindicators of the quality of water treated by means of Bioremediation with the microorganism Bacillus subtilis and treated water in the UPIBA. The experiments\' results showed that the kefir grains generated different biological responses when submitted to different concentrations of benzene, present in bioremediated waters with B. subtilis. And trials applying K. pinnata as a bioindicator of rainwater quality and SABESP, have suggested that it may be an excellent bioindicator in eutrophic waters, that is, rich in organic matter.
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Almutairi, Hayfa Habes. "Investigations of protein structure-function relationships." Bowling Green State University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1530204962869878.
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Mathiba, Matsobane Taboga. "Influence of heat, aluminium toxicity and exposure to Bacillus subtilis on the germination of Abelmoschus esculentus." Diss., 2015. http://hdl.handle.net/10500/19972.
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Okra (Abelmuschus esculentus (L) Moench.) is one of the most popular crops within the Malvaceae family of plants. It is a common vegetable eminently cultivated in regions experiencing constraints to manage climate change. In South Africa climate change coupled with aluminium-enriched soils are responsible to drawbacks crop performance. Therefore, it is worthwhile to whether okra will thrive as an alternative crop in the country. Many studies have identified potential of okra to improve yields of resource poor farmers in Africa. The physiological responses of okra seed to variations in aluminium ions and temperature were not determined. Therefore, a study with okra, cv. Clemson Spineless, seed coated and uncoated with B. subtilis, was initiated to assess germination on moist filter paper in 90mm diameter Petri plates. Germination medium consisted of various concentrations of aluminium chloride (AlCl3), 0M, 0.001M, 0.01M, 0.05M and 0.1M. Each aluminium treatment was allocated into incubators adjusted to 22°C, 25°C and 37°C temperatures. This resulted into a 5 x 3 x 2 factorial experiment with five replicates and was conducted in three cycles. Daily scores of germinated seeds were assessed from the second to the fifth day after initiation of germination. During termination, five days after the initiation of the experiment 10 seeds with the longest coleoptiles had their coleoptiles measured using a digital caliper. At the fifth day after initiation of the experiment, coleoptile lengths from 10 seeds per treatment were measured using digital caliper. A total of 50 plates (10 from 37°C in Cycle 1; 30 from 22°C, 25°C and 37°C from Cycle 2; 10 from 37°C in Cycle 3), were selected and germinated were ground and stored at - 20°C before 1H NMR analysis. Metabolites were extracted from 50mg ground seed material with 750 μL methanol-D4 and 750 μL buffer (deuterium oxide + potassium dihydrogen phosphate). The mixture was vortexed for three minutes, sonicated for 20 minutes, centrifuged at 18000 rpms for 20 minutes and the supernatant filtered through cotton wool. Then the supernatant was dispensed into NMR tubes for further 1H NMR spectroscopic processing using a 600 MHz NMR xiii
Varian spectrometer to generate magnetic spectra of the fifty samples. Results of this study demonstrated that in all the experimental cycles, regardless of aluminium concentration and bacterial seed coating, 37°C inhibited germination percentages and coleoptile lengths in okra seed germination. Germination percentages and coleoptile lengths of bacteria-coated seeds growing in 25°C were most stimulated at all aluminium concentrations, but not at 0.1M. In this temperature germination percentages and coleoptile lengths were highly influenced by the interaction of aluminium concentrations and bacterial coating, respectively. 1H NMR metabolomic association showed no distinct grouping, but clusters across treatments showed to be linked through a subset of metabolites amongst aluminium concentrations, bacterial seed coating and temperatures, respectively. This infers that treatment variations in both seed and bacterial physiological responses were associated through shared metabolic pathways. In conclusion, the study proved that 25°C provide temperature environment within which B. subtilis can be able to stimulate growth and remediate physiological constraints from aluminium ions during okra seed germination.Agriculture, Animal Health and Human Ecology
M. Sc. (Agriculture)
Book chapters on the topic "Bacillus subtilis metal bioremediation"
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Herbig, Andrew F., and John D. Helmann. "Metal Ion Uptake and Oxidative Stress." In Bacillus subtilis and Its Closest Relatives, 405–14. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817992.ch29.
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Krishna, M. P., Rinoy Varghese, V. Arun Babu, S. Jyothy, and A. A. Mohamed Hatha. "Bioremediation of Zinc Using Bacillus sp. Isolated from Metal-Contaminated Industrial Zone." In Prospects in Bioscience: Addressing the Issues, 11–18. India: Springer India, 2012. http://dx.doi.org/10.1007/978-81-322-0810-5_2.
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Kanmani, P., J. Aravind, and K. Kumaresan. "Hydrolytic Enzyme Profiling of Bacillus Subtilis COM6B and Its Application in the Bioremediation of Groundnut Oil Mill Effluent." In Integrated Waste Management in India, 179–89. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-27228-3_17.
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Pal, Amit Kumar, Anjan Hazra, and Chandan Sengupta. "Cadmium- and Lead-Tolerant PGPRs as Proficient Toxicity Alleviators for Agricultural Crops." In Recent Advancements in Bioremediation of Metal Contaminants, 189–204. IGI Global, 2021. http://dx.doi.org/10.4018/978-1-7998-4888-2.ch010.
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Agricultural lands are being polluted with different contaminants due to various anthropogenic activities like toxic discharge from Ni-Cd battery industry, tannery industry, alloying of metals like steel, application of agrochemicals, etc. Cadmium and lead contamination in agricultural land are directed towards global food insecurity. Bioremediation, stress alleviation, and phytostimulation by Cd and Pb tolerant PGPR is a promising eco-friendly method to develop sustainable agricultural system. At present, cadmium and lead-tolerant plant growth promoting rhizobacteria (PGPR) can be a sustainable option for heavy metal-contaminated agricultural lands. PGPRs such as Bacillus, Bradyrhizobium, Enterobacter, Klebsiella, Micrococcus, Pseudomonas, Ralstonia, etc. can survive the metal stress and stimulate the plant growth under Cd and Pb contaminated condition by direct or indirect plant growth promoting ability. So, these PGPRs could be exploited as biofertilizers and bioremediators under Cd or Pb stressed conditions for futuristic agricultural development.
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Roy, Dipankar, and Arup Kumar Mitra. "Bacterial Remediation of Chromium From Industrial Sludge." In Recent Advancements in Bioremediation of Metal Contaminants, 97–125. IGI Global, 2021. http://dx.doi.org/10.4018/978-1-7998-4888-2.ch006.
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Chromium-like heavy toxic metals seriously influence the metabolism of living organisms and cause permanent threatening of health. Microorganisms can help to detoxify those hazardous heavy metals in the environment by the process of bioremediation. Two bacterial genera were isolated from industrial sludge designated P1 and P2. From the 16srRNA study, it is revealed that P1 is Bacillus cereus and P2 is Enterobacter sp. They are deposited in NCMR and NCBI and received the accession no. MCC 3868 for P1 and MCC 3788 for P2. P1 is gram positive, motile, and P2 is gram negative, motile. Eighteen antibiotics have been taken for antibiotic assay; P1 is resistant to 12; P2 is resistant to 8 antibiotics. For growth pattern analysis in chromium, three parameters have been selected, and they are temperature, pH, and biomass. In LD50 and above parameters, total chromium uptake by those bacteria in stressed conditions have been recorded. The two bacteria are not antagonistic to each other so they are used to bioremediate chromium from their contaminated sites and also treated as consortium.
Conference papers on the topic "Bacillus subtilis metal bioremediation"
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Mardiyono and Nur Hidayati. "Bioremediation of chrome heavy metals on metal coating waste with Bacillus subtilis bacteria." In INTERNATIONAL CONFERENCE ON SCIENCE AND APPLIED SCIENCE (ICSAS2020). AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0030561.
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Mardiyono, Sajidan, M. Masykuri, and P. Setyono. "Bioremediation of nickel heavy metals in electroplating industrial liquid waste with Bacillus subtilis." In INTERNATIONAL CONFERENCE ON SCIENCE AND APPLIED SCIENCE (ICSAS) 2019. AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5141697.
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Fang, Di, and Jie Li. "Notice of Retraction: Isolation of Heterotrophic Microorganism Bacillus subtilis Lj86 for Improving Autotrophic Sulfur-Based Bioleaching of Metal-Polluted Sediments." In 2011 5th International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5780788.
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