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Journal articles on the topic 'BACTEC 460/MGIT-TB'

Author: Grafiati
Published: 2 June 2025
Last updated: 31 July 2025

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1

Hanna, Bruce A., Adeleh Ebrahimzadeh, L. Bruce Elliott, et al. "Multicenter Evaluation of the BACTEC MGIT 960 System for Recovery of Mycobacteria." Journal of Clinical Microbiology 37, no. 3 (1999): 748–52. http://dx.doi.org/10.1128/jcm.37.3.748-752.1999.

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We evaluated the BACTEC MGIT 960 system, which is a fully automated, noninvasive system for the growth and detection of mycobacteria with a capacity to incubate and continuously monitor 960 7-ml culture tubes. We studied 3,330 specimens, 2,210 respiratory and 1,120 nonrespiratory specimens, collected from 2,346 patients treated at six sites. Processed specimens were inoculated into the BACTEC MGIT 960 and BACTEC 460 TB systems, as well as onto Lowenstein-Jensen slants and Middlebrook 7H11/7H11 selective plates. From all culture systems, a total of 362 isolates of mycobacteria were recovered; t
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2

Werngren, J., E. Sturegård, P. Juréen, K. Ängeby, S. Hoffner, and T. Schön. "Reevaluation of the Critical Concentration for Drug Susceptibility Testing of Mycobacterium tuberculosis against Pyrazinamide Using Wild-Type MIC Distributions andpncAGene Sequencing." Antimicrobial Agents and Chemotherapy 56, no. 3 (2011): 1253–57. http://dx.doi.org/10.1128/aac.05894-11.

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ABSTRACTPyrazinamide (PZA) is a potent first-line agent for the treatment of tuberculosis (TB) with activity also against a significant part of drug-resistantMycobacterium tuberculosisstrains. Since PZA is active only at acid pH, testing for susceptibility to PZA is difficult and insufficiently reproducible. The recommended critical concentration for PZA susceptibility (MIC, 100 mg/liter) used in the Bactec systems (460 and MGIT 960) has not been critically evaluated against wild-type MIC distributions in clinical isolates ofMycobacterium tuberculosis. Using the Bactec MGIT 960 system, we dete
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3

Maningi, Nontuthuko E., Luke T. Daum, John D. Rodriguez, et al. "Improved Detection by Next-Generation Sequencing of Pyrazinamide Resistance in Mycobacterium tuberculosis Isolates." Journal of Clinical Microbiology 53, no. 12 (2015): 3779–83. http://dx.doi.org/10.1128/jcm.01179-15.

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The technical limitations of common tests used for detecting pyrazinamide (PZA) resistance inMycobacterium tuberculosisisolates pose challenges for comprehensive and accurate descriptions of drug resistance in patients with multidrug-resistant tuberculosis (MDR-TB). In this study, a 606-bp fragment (comprising thepncAcoding region plus the promoter) was sequenced using Ion Torrent next-generation sequencing (NGS) to detect associated PZA resistance mutations in 88 recultured MDR-TB isolates from an archived series collected in 2001. These 88 isolates were previously Sanger sequenced, with 55 (
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4

Cruciani, M., C. Scarparo, M. Malena, O. Bosco, G. Serpelloni, and C. Mengoli. "Meta-Analysis of BACTEC MGIT 960 and BACTEC 460 TB, with or without Solid Media, for Detection of Mycobacteria." Journal of Clinical Microbiology 42, no. 5 (2004): 2321–25. http://dx.doi.org/10.1128/jcm.42.5.2321-2325.2004.

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5

Scarparo, Claudio, Paola Piccoli, Alessandra Rigon, Giuliana Ruggiero, Paolo Ricordi, and Claudio Piersimoni. "Evaluation of the BACTEC MGIT 960 in comparison with BACTEC 460 TB for detection and recovery of mycobacteria from clinical specimens." Diagnostic Microbiology and Infectious Disease 44, no. 2 (2002): 157–61. http://dx.doi.org/10.1016/s0732-8893(02)00437-6.

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6

Payaslıoğlu, Melda, İmran Sağlık, and Cüneyt Özakın. "Resistance Rates of Mycobacterium tuberculosis Complex Strains: A Retrospective Study in Türkiye." Medicina 61, no. 6 (2025): 1060. https://doi.org/10.3390/medicina61061060.

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Background and Objectives: Tuberculosis (TB) is one of the most common infectious diseases in developing countries. The resistance of the causative agent, Mycobacterium tuberculosis, to two or more first-line anti-TB drugs results in multidrug-resistant (MDR) TB, posing a serious challenge to the control of TB worldwide. This study was designed to determine the changes in drug resistance over time in TB strains isolated from patients in all departments of Uludağ University Hospital in western Türkiye. Materials and Methods: We retrospectively analyzed 104,598 clinical samples sent to our labor
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7

Somoskövi, Ákos, Csaba Ködmön, Ákos Lantos, et al. "Comparison of Recoveries of Mycobacterium tuberculosis Using the Automated BACTEC MGIT 960 System, the BACTEC 460 TB System, and Löwenstein-Jensen Medium." Journal of Clinical Microbiology 38, no. 6 (2000): 2395–97. http://dx.doi.org/10.1128/jcm.38.6.2395-2397.2000.

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8

Somoskövi, Ákos, Csaba Ködmön, Ákos Lantos, et al. "Comparison of Recoveries of Mycobacterium tuberculosis Using the Automated BACTEC MGIT 960 System, the BACTEC 460 TB System, and Löwenstein-Jensen Medium." Journal of Clinical Microbiology 38, no. 6 (2000): 2395–97. http://dx.doi.org/10.1128/.38.6.2395-2397.2000.

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9

Robbe-Austerman, Suelee, Doris M. Bravo, and Beth Harris. "Comparison of the MGIT 960, BACTEC 460 TB and solid media for isolation of Mycobacterium bovis in United States veterinary specimens." BMC Veterinary Research 9, no. 1 (2013): 74. http://dx.doi.org/10.1186/1746-6148-9-74.

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10

Bergmann, John S., Geoffrey Fish, and Gail L. Woods. "Evaluation of the BBL MGIT (Mycobacterial Growth Indicator Tube) AST SIRE System for Antimycobacterial Susceptibility Testing of Mycobacterium tuberculosis to 4 Primary Antituberculous Drugs." Archives of Pathology & Laboratory Medicine 124, no. 1 (2000): 82–86. http://dx.doi.org/10.5858/2000-124-0082-eotbmm.

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Abstract Objective.—To evaluate the performance of the BBL MGIT (Mycobacterial Growth Indicator Tube) AST SIRE system for the antimycobacterial susceptibility testing of Mycobacterium tuberculosis to isoniazid (at a concentration equivalent to the lower concentration used for testing by the method of proportion), rifampin, ethambutol, and streptomycin. Design.—Thirty-one clinical isolates and 30 challenge strains provided by the Centers for Disease Control and Prevention (CDC) were tested by MGIT AST SIRE using 2 methods of inoculum preparation, and results were compared with those of the meth
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11

Arbab Nasr, Mohamed H., Abdullahi Nor Hasan, Tarig M. S Alnour, and Eltayib H. Ahmed. "Evaluation of Newly Modified Potassium Nitrate Containing Media in Detection and Antibiogram of Mycobacterium tuberculosis." SVOA Microbiology 5, no. 4 (2024): 122–30. http://dx.doi.org/10.58624/svoamb.2024.05.049.

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Background: Mycobacterium is a genus of Actinobacteria, given its own family, the Mycobacteriaceae. The genus includes pathogens known to cause serious diseases in mammals, including tuberculosis (Mycobacterium tuberculosis) and the classic Hansen's strain of leprosy (Mycobacterium leprae) [1]. Tuberculosis is the most common life-threatening opportunistic infection (OI) and AIDS defining illness in developing countries [2]. The routine identification methods of tuberculosis includes, Tuberculin skin test, Chest X-ray, Growth and physical characteristics of the bacteria, automated based system
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12

Mokaddas, Eiman, Suhail Ahmad, Hanaa S. Eldeen, and Noura Al-Mutairi. "Discordance between Xpert MTB/RIF Assay and Bactec MGIT 960 Culture System for Detection of Rifampin-Resistant Mycobacterium tuberculosis Isolates in a Country with a Low Tuberculosis (TB) Incidence." Journal of Clinical Microbiology 53, no. 4 (2015): 1351–54. http://dx.doi.org/10.1128/jcm.03412-14.

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Among 452 samples that were positive by the Xpert MTB/RIF (Xpert) assay and MGIT 960 system (MGIT), 440 and 10Mycobacterium tuberculosissamples were detected as rifampin susceptible and rifampin resistant, respectively. Two isolates that were rifampin susceptible by the MGIT system were rifampin resistant by the Xpert assay.rpoBsequencing identified a silent (CTG521TTG) mutation in one isolate and a missense (GAC516TAC) mutation in another. The detection of rifampin resistance is imperfect with both the Xpert assay and MGIT system. Any discordant rifampin resistance results should be confirmed
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13

Trivedi, Mahendra Kumar, Shrikant Patil, Harish Shettigar, Sambhu Charan Mondal, and Snehasis Jana. "An Impact of Biofield Treatment: Antimycobacterial Susceptibility Potential Using BACTEC 460/MGIT-TB System." Mycobacterial Diseases 5, no. 4 (2015). https://doi.org/10.4172/2161-1068.1000189.

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The aim was to evaluate the impact of biofield treatment modality on mycobacterial strains in relation to antimycobacterials susceptibility. Mycobacterial sensitivity was analysed using 12 B BACTEC vials on the BACTEC 460 TB machine in 39 lab isolates (sputum samples) from stored stock cultures. Two American Type Culture Collection (ATCC) strains were also used to assess the minimum inhibitory concentration (MIC) of antimicrobials (Mycobacterium smegmatis 14468 and Mycobacterium tuberculosis 25177). Rifampicin, ethambutol and streptomycin in treated samples showed increased susceptibility as 3
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14

Trivedi, Mahendra. "An Impact of Biofield treatment: Antimycobacterial Susceptibility Potential Using BACTEC 460/MGIT-TB System." OMICS International, July 27, 2015. https://doi.org/10.5281/zenodo.813388.

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The aim was to evaluate the impact of biofield treatment modality on mycobacterial strains in relation to antimycobacterials susceptibility. Mycobacterial sensitivity was analysed using 12 B BACTEC vials on the BACTEC 460 TB machine in 39 lab isolates (sputum samples) from stored stock cultures. Two American Type Culture Collection (ATCC) strains were also used to assess the minimum inhibitory concentration (MIC) of antimicrobials (Mycobacterium smegmatis 14468 and Mycobacterium tuberculosis 25177). Rifampicin, ethambutol and streptomycin in treated samples showed increased susceptibility as 3
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15

Kumar Trivedi, Mahendra. "An Impact of Biofield Treatment: Antimycobacterial Susceptibility Potential Using BACTEC 460/MGIT-TB System." Mycobacterial Diseases 05, no. 04 (2015). http://dx.doi.org/10.4172/2161-1068.1000189.

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