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Journal articles on the topic 'BACTEC 960'

Author: Grafiati
Published: 2 June 2025
Last updated: 31 July 2025

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1

Hanna, Bruce A., Adeleh Ebrahimzadeh, L. Bruce Elliott, et al. "Multicenter Evaluation of the BACTEC MGIT 960 System for Recovery of Mycobacteria." Journal of Clinical Microbiology 37, no. 3 (1999): 748–52. http://dx.doi.org/10.1128/jcm.37.3.748-752.1999.

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We evaluated the BACTEC MGIT 960 system, which is a fully automated, noninvasive system for the growth and detection of mycobacteria with a capacity to incubate and continuously monitor 960 7-ml culture tubes. We studied 3,330 specimens, 2,210 respiratory and 1,120 nonrespiratory specimens, collected from 2,346 patients treated at six sites. Processed specimens were inoculated into the BACTEC MGIT 960 and BACTEC 460 TB systems, as well as onto Lowenstein-Jensen slants and Middlebrook 7H11/7H11 selective plates. From all culture systems, a total of 362 isolates of mycobacteria were recovered; t
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2

A. Abdul Kareem, Mo A. AL-Mazini, T. Bukeet, and. "Comparison of recoveries of Mycobacterium tuberculosis using the automated BACTEC MGIT 960 System and Lowenstein – Jensen medium." Al-Qadisiyah Journal of Veterinary Medicine Sciences 9, no. 1 (2010): 44. http://dx.doi.org/10.29079/vol9iss1art92.

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We examined whether the BACTEC/ Mycobacteria Growth Indicator Tube (MGIT) System alone could supplant the use of a supplemental Lowenstein–Jensen (LJ) slant for routine recovery of M. tuberculosis from clinical specimens. A total of 392 specimens of sputum were included in the study, collected from 196 patients. Specimens were processed with standard N-acetyl- L- Cysteine (NALC-NaOH) method, then inoculated onto BACTEC MGIT 960 and onto LJ media. The recovery rates of M.tuberculosis were 100 % (256/256) with BACTEC MGIT 960 and 72.6%(186/256) with LJ. The rates of contamination for each of the
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3

Williams-Bouyer, Natalie, Rebecca Yorke, Hung I. Lee, and Gail L. Woods. "Comparison of the BACTEC MGIT 960 and ESP Culture System II for Growth and Detection of Mycobacteria." Journal of Clinical Microbiology 38, no. 11 (2000): 4167–70. http://dx.doi.org/10.1128/jcm.38.11.4167-4170.2000.

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The performances of two continuously monitoring mycobacterial culture systems—ESP Culture System II (ESP II; Trek Diagnostics, Inc., Westlake, Ohio) and BACTEC MGIT 960 (BD Biosciences, Sparks, Md.)—were compared. In addition to both liquid media, all specimens were plated onto Middlebrook 7H11/7H11 selective agar. A total of 3,151 specimens of all types (56.3% were respiratory specimens) were cultured; 231 (7.3%) yielded mycobacteria. The most common species recovered wereMycobacterium avium complex (69 isolates) andMycobacterium tuberculosis complex (MTBC; 65 isolates). The recovery rates fo
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Presialia, Athira, and Ariyani Kiranasari. "Perbandingan Tingkat Kontaminasi Kultur Mycobacterium tuberculosis pada Medium Lowenstein-Jensen dan BACTEC MGIT 960." Jurnal Kedokteran Meditek 26, no. 2 (2020): 36–42. http://dx.doi.org/10.36452/jkdoktmeditek.v26i2.1829.

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Sampai saat ini, kultur masih merupakan standar pemeriksaan diagnosis tuberkulosis. Metode yang sering dilakukan adalah menggunakan Medium Lowenstein-Jensen dan BACTEC MGIT 960, namun kontaminasi oleh bakteri lain maupun jamur dapat terjadi dan dapat mengganggu hasil interpretasi. Penelitian ini bertujuan untuk mengetahui perbandingan tingkat kontaminasi kultur Mycobacterium tuberculosis pada Medium Lowenstein-Jensen dan BACTEC MGIT 960. Penelitian ini menggunakan desain studi potong lintang dengan 204 sampel dari Laboratorium Mikrobiologi Klinik FKUI-RSCM yang memenuhi kriteria penelitian. Da
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Tortoli, Enrico, Paola Cichero, Claudio Piersimoni, M. Tullia Simonetti, Giampietro Gesu, and Domenico Nista. "Use of BACTEC MGIT 960 for Recovery of Mycobacteria from Clinical Specimens: Multicenter Study." Journal of Clinical Microbiology 37, no. 11 (1999): 3578–82. http://dx.doi.org/10.1128/jcm.37.11.3578-3582.1999.

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The BACTEC MGIT 960 instrument is a fully automated system that exploits the fluorescence of an oxygen sensor to detect growth of mycobacteria in culture. Its performance was compared to those of the radiometric BACTEC 460 instrument and egg-based Lowenstein-Jensen medium. An identical volume of sample was inoculated in different media, and incubation was carried out for 6 weeks with the automatic systems and for 8 weeks on solid media. A total of 2,567 specimens obtained from 1,631 patients were cultured in parallel. Mycobacteria belonging to nine different taxa were isolated by at least one
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Barreto, Leonardo Bruno Paz Ferreira, Maria Cristina da Silva Lourenço, Valéria Cavalcanti Rolla, Valdiléia Gonçalves Veloso, and Gisele Huf. "Use of amplified Mycobacterium tuberculosis direct test in respiratory samples from HIV-infected patients in Brazil." Jornal Brasileiro de Pneumologia 40, no. 2 (2014): 148–54. http://dx.doi.org/10.1590/s1806-37132014000200008.

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OBJECTIVE: To compare the accuracy of the amplified Mycobacterium tuberculosis direct (AMTD) test with reference methods for the laboratory diagnosis of tuberculosis in HIV-infected patients. METHODS: This was a study of diagnostic accuracy comparing AMTD test results with those obtained by culture on Löwenstein-Jensen (LJ) medium and by the BACTEC Mycobacteria Growth Indicator Tube 960 (BACTEC MGIT 960) system in respiratory samples analyzed at the Bioassay and Bacteriology Laboratory of the Oswaldo Cruz Foundation Evandro Chagas Clinical Research Institute in the city of Rio de Janeiro, Braz
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7

Whyte, Tom, Martin Cormican, Belinda Hanahoe, Geraldine Doran, Tom Collins, and Geraldine Corbett-Feeney. "Comparison of BACTEC MGIT 960 and BACTEC 460 for culture of Mycobacteria." Diagnostic Microbiology and Infectious Disease 38, no. 2 (2000): 123–26. http://dx.doi.org/10.1016/s0732-8893(00)00179-6.

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8

de Almeida, Isabela Neves, Suely Conceição Alves da Silva, Haliton Alves de Oliveira Junior, et al. "The Activity-Based Cost of Drug-Susceptibility Test of Mycobacterium tuberculosis Through Kit SIRE Nitratase® Plastlabor." International Journal of Mycobacteriology 9, no. 1 (2020): 24–28. http://dx.doi.org/10.4103/ijmy.ijmy_8_20.

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Background: Drug-resistant tuberculosis (TB) is an ongoing health threat, and the greatest challenge to adequate control of TB in many countries lies in the lack of proper laboratory drug-susceptibility test. The aim of this study was to evaluate the activity-based costs (ABC) of Kit SIRE Nitratase® (Kit SIRE) and compare its values with the conventional drug-susceptibility test. Methods: The ABC was calculated for three different approaches: Kit SIRE (clinical samples and cultures), proportion methods in Lowenstein Jensen (PM-LJ), and the Bactec™ MGIT™ 960 system based on Mycobacterial Resear
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9

Alcaide, Fernando, Miguel Angel Benítez, Josep M. Escribà, and Rogelio Martín. "Evaluation of the BACTEC MGIT 960 and the MB/BacT Systems for Recovery of Mycobacteria from Clinical Specimens and for Species Identification by DNA AccuProbe." Journal of Clinical Microbiology 38, no. 1 (2000): 398–401. http://dx.doi.org/10.1128/jcm.38.1.398-401.2000.

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ABSTRACT A total of 120 mycobacterial isolates were recovered from 1,068 clinical specimens. Of these, 82.5% were in MGIT 960, 83.3% were in MB/BacT, 80% were in BACTEC 460, and 70% were on Löwenstein-Jensen medium. Mean times to detection of Mycobacterium tuberculosis ( n = 96) were significantly shorter with MGIT 960 (12.6 days, P = 0.003) and BACTEC 460 (11.8 days, P < 0.001) than with MB/BacT (15.9 days). Although, MGIT 960 showed the lowest rate of recovery of M. kansasii genotype I (64.3%), the earliest growth was detected with this system (8.9 days). Low and similar rates of contami
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10

Werngren, J., E. Sturegård, P. Juréen, K. Ängeby, S. Hoffner, and T. Schön. "Reevaluation of the Critical Concentration for Drug Susceptibility Testing of Mycobacterium tuberculosis against Pyrazinamide Using Wild-Type MIC Distributions andpncAGene Sequencing." Antimicrobial Agents and Chemotherapy 56, no. 3 (2011): 1253–57. http://dx.doi.org/10.1128/aac.05894-11.

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ABSTRACTPyrazinamide (PZA) is a potent first-line agent for the treatment of tuberculosis (TB) with activity also against a significant part of drug-resistantMycobacterium tuberculosisstrains. Since PZA is active only at acid pH, testing for susceptibility to PZA is difficult and insufficiently reproducible. The recommended critical concentration for PZA susceptibility (MIC, 100 mg/liter) used in the Bactec systems (460 and MGIT 960) has not been critically evaluated against wild-type MIC distributions in clinical isolates ofMycobacterium tuberculosis. Using the Bactec MGIT 960 system, we dete
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11

Rufai, Syed Beenish, Amit Singh, Parveen Kumar, Jitendra Singh, and Sarman Singh. "Performance of Xpert MTB/RIF Assay in Diagnosis of Pleural Tuberculosis by Use of Pleural Fluid Samples." Journal of Clinical Microbiology 53, no. 11 (2015): 3636–38. http://dx.doi.org/10.1128/jcm.02182-15.

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Prospectively, 162 pleural fluid samples from patients with probable tuberculous pleural effusion were tested by the Xpert MTB/RIF assay and the Bactec MGIT-960 culture system. Of these, 43 (26.5%) were positive in the MGIT-960 culture, and 23 (14.2%), in the Xpert MTB/RIF assay. The sensitivity and specificity of the Xpert MTB/RIF compared with the MGIT-960 culture were 54.8% and 100%, respectively.
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12

Thakur, Rajeev, Renu Goyal, and Smita Sarma. "Laboratory Diagnosis of Tuberculous Meningitis – Is There a Scope for Further Improvement?" Journal of Laboratory Physicians 2, no. 01 (2010): 021–24. http://dx.doi.org/10.4103/0974-2727.66705.

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ABSTRACT Aims: Tuberculous meningitis (TBM) still remains a diagnostic challenge because of inconsistent clinical presentation and lack of rapid, sensitive and specific tests. This study was carried out to diagnose TBM by a combination of direct microscopy on Ziehl-Neelsen (ZN) staining, culture by conventional Lowenstein Jensen (LJ) media and Bactec MGIT 960 system in clinically suspected cases, supported by laboratory parameters. Materials and Methods: A total of 164 cerebrospinal fluid (CSF) samples from suspected cases of TBM were processed for direct acid fast bacilli (AFB) smear examinat
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13

Mikhaylova, I. G., E. I. Slynko, E. S. Grechko, T. А. Rastorgueva, and M. B. Pikalova. "EXPERIENCE OF USING BACTEC MGIT 960 BY TIME FRAMES FOR CULTURE." TUBERCULOSIS AND LUNG DISEASES 97, no. 5 (2019): 74–75. http://dx.doi.org/10.21292/2075-1230-2019-97-5-74-75.

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14

Севастьянова, Е. В., Т. Г. Смирнова, Е. Е. Ларионова та Л. Н. Черноусова. "КУЛЬТУРАЛЬНЫЙ МЕТОД ИССЛЕДОВАНИЯ МИКОБАКТЕРИЙ. ЖИДКИЕ ПИТАТЕЛЬНЫЕ СРЕДЫ И АВТОМАТИЗИРОВАННЫЕ СИСТЕМЫ, "Вестник Центрального научно-исследовательского института туберкулеза"". Вестник ЦНИИТ, № 4 (2020): 88–95. http://dx.doi.org/10.7868/s258766782004010x.

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15

Umpeleva, T. V., E. A. Mazurina, D. V. Vakhrusheva, and N. I. Eremeeva. "Comparison of Different Methods for Drug Susceptibility Testing of Mycobacterium tuberculosis to Rifampicin." Tuberculosis and Lung Diseases 100, no. 1 (2022): 41–48. http://dx.doi.org/10.21292/2075-1230-2022-100-1-41-48.

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The objective: to compare results of drug susceptibility testing to rifampicin by molecular genetic methods and phenotypic tests of Mycobacterium tuberculosis isolates obtained from clinical specimens of tuberculosis patients.Subjects and Methods. 915 samples of M. tuberculosis DNA and 426 cultures were used in this study. Genotypic tests (TB-TEST (BIOCIP-IMB, Russia), GenoType MTBDRplusV2) and phenotypic technologies (absolute concentration method, Bactec MGIT 960 system, Sensititre Myco TB kit) were used.Results. A high percentage (98.7%; CI 97.7-99.7%) of confirmation of the results of the
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Kadhim, Afraa Ali, Mohammed Faraj AL-Marjan, Kadhim Ali Kadhim, and Ahmed Asmar Manki. "Isolation of Mycobacterium tuberculosis and testing their susceptibility to antimicrobial agents by using Bactec 960." Al Mustansiriyah Journal of Pharmaceutical Sciences 13, no. 1 (2013): 175–81. http://dx.doi.org/10.32947/ajps.v13i1.213.

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This study was done from 3/7/2011 to 1/1/2012 , different clinical samples were collected from (1235) patients that coming to the Specialized center for chest and respiratory diseases / Baghdad to investigate Mycobacterium tuberculosis and study their resistant to first and second line antimicrobial agents by using Bactec 960.The results obtained from direct smear examination with Ziehl – Neelsen stain in (294) patients that indicated these patients are Infected with tuberculosis . These samples has been cultured on solid medium Lowenstein Jensen media (L.J.) and liquid medium ( middle brook 7
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Thuansuwan, Waraporn, Charoen Chuchottaworn, Chie Nakajima, Yasuhiko Suzuki, and Nuntaree Chaichanawongsaroj. "Biphasic Medium Using Nicotinamide for Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis." Antibiotics 13, no. 6 (2024): 563. http://dx.doi.org/10.3390/antibiotics13060563.

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Reliable drug susceptibility testing of pyrazinamide (PZA) is technically difficult, since PZA activity is pH sensitive. The aim of this study was to evaluate a biphasic medium assay (BMA) for the reliable detection of PZA resistance in Mycobacterium tuberculosis (MTB) using nicotinamide (NIC) as a surrogate for PZA and identifying the appropriate cut-off value for the assay. The PZA susceptibility of 122 multidrug-resistant tuberculosis (MDR-TB) isolates and 39 drug-susceptible tuberculosis (DS-TB) isolates was examined using the BMA with NIC at four different concentrations (250, 500, 1000,
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Owusu, Enid, and Mercy Jemima Newman. "Microscopic Observation Drug Susceptibility (MODS) Assay: A Convenient Method for Determining Antibiogram of Clinical Isolates of Mycobacterium tuberculosis in Ghana." Medical Sciences 8, no. 1 (2020): 5. http://dx.doi.org/10.3390/medsci8010005.

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(1) Background: Present methods for drug susceptibility tests (DST) rely on culture methods that are sophisticated and relatively faster, or a slow and cheaper option. These methods frustrate disease control; therefore, there is a need for methods that incorporate key functions of microscopy and culture, with reduced cost burden and sophistry. Thus, the purpose of this study was to identify which, among the most commonly used (in Ghana) methods, can conveniently be used at health centers located in rural areas for effective DST determination of Mycobacterium tuberculosis (MTB). (2) Methods: My
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Domotenko, L. V., T. P. Morozova, I. G. Shemyakin, and A. P. Shepelin. "EXPERIENCE OF USING TB TEST KIT FOR THE RAPID DRUG SUSCEPTIBILITY TESTING OF M. TUBERCULOSIS." Russian Clinical Laboratory Diagnostics 65, no. 2 (2020): 122–30. http://dx.doi.org/10.18821/0869-2084-2020-65-2-122-130.

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The results of the comparative testing of the susceptibility of M. tuberculosis clinical strains to isoniazid, streptomycin, rifampicin and ethambutol using the TB test kit, developed in SCRAMB, (Obolensk) and the absolute concentrations method; the TB test kit and the BACTEC MGIT 960 automated system are presented in the study. A total of 629 and 220 strains, respectively, were tested. A high degree of agreement of the results was shown: 89.1-98.6% for isoniazid, 96.2-98.0% for rifampicin, 91.5-98.2% for streptomycin and 89.1-95.9% for ethambutol. The smallest number of discrepancies in the r
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Pang, Yu, Biyi Su, Huiwen Zheng, et al. "Factors Associated with Missed Detection ofMycobacterium tuberculosisby Automated BACTEC MGIT 960 System." BioMed Research International 2016 (2016): 1–4. http://dx.doi.org/10.1155/2016/5972021.

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Despite the demonstration of excellent performance, mycobacterial growth in BACTEC MGIT 960 can go undetected. The aim of this study was to investigate the prevalence of “false-negative” culture sample in Beijing and the potential factors associated with the detection failures by MGIT 960. Of the 577 sputum samples tested, 141 (24.4%) were culture-positive for mycobacteria, of which 133 (94.3%) were automatically determined by MGIT 960 system and 8 (5.7%) were positive for visual growth (false negative by MGIT). Statistical analysis showed that positive grade of specimen had no influence on th
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21

Heysell, Scott K., Suporn Pholwat, Stellah G. Mpagama, et al. "Sensititre MycoTB Plate Compared to Bactec MGIT 960 for First- and Second-Line Antituberculosis Drug Susceptibility Testing in Tanzania: a Call To Operationalize MICs." Antimicrobial Agents and Chemotherapy 59, no. 11 (2015): 7104–8. http://dx.doi.org/10.1128/aac.01117-15.

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ABSTRACTMIC testing forMycobacterium tuberculosisis now commercially available. Drug susceptibility testing by the MycoTB MIC plate has not been directly compared to that by the Bactec MGIT 960. We describe a case of extensively drug-resistant tuberculosis (XDR-TB) in Tanzania where initial MIC testing may have prevented acquired resistance. From testing on archived isolates, the accuracy with the MycoTB plate was >90% for important first- and second-line drugs compared to that with the MGIT 960, and clinically useful quantitative interpretation was also provided.
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22

Eliseev, P. I., E. I. Nikishova, A. Yu Krupskaya, V. I. Shtraukh, E. S. Khimova, and A. O. Maryandyshev. "Detection of <i>M. tuberculosis</i> Using Cartridge-Based Technology in Patients with Negative Sputum Microscopy Results in a Regional Laboratory." Tuberculosis and Lung Diseases 102, no. 2 (2024): 36–42. http://dx.doi.org/10.58838/2075-1230-2024-102-2-36-42.

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The objective: to evaluate results of M. tuberculosis detection and testing drug susceptibility to rifampicin using molecular genetic methods in tuberculosis patients with a negative sputum smear microscopy result.Subjects and Methods. Pulmonary tuberculosis patients registered in Arkhangelsk Region from 2016 to 2020 were enrolled in the study; those patients were notified as new cases and relapses with a negative sputum smear microscopy result before treatment and had results of molecular genetic testing (MGT) (Xpert MTB/RIF MTB/RIF) and BACTEC MGIT 960 available.Results. 479 people (40%) wer
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23

Cruciani, M., C. Scarparo, M. Malena, O. Bosco, G. Serpelloni, and C. Mengoli. "Meta-Analysis of BACTEC MGIT 960 and BACTEC 460 TB, with or without Solid Media, for Detection of Mycobacteria." Journal of Clinical Microbiology 42, no. 5 (2004): 2321–25. http://dx.doi.org/10.1128/jcm.42.5.2321-2325.2004.

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Pinheiro, M. D., and M. M. Ribeiro. "Comparison of the Bactec 460TB system and the Bactec MGIT 960 system in recovery of mycobacteria from clinical specimens." Clinical Microbiology and Infection 6, no. 3 (2000): 171–73. http://dx.doi.org/10.1046/j.1469-0691.2000.00036-4.x.

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Hannan, Abdul, Saira Munir, Muhammad Usman Arshad, and Nabila Bashir. "In Vitro Antimycobacterial Activity of Pakistani Beri Honey Using BACTEC MGIT 960." International Scholarly Research Notices 2014 (October 7, 2014): 1–4. http://dx.doi.org/10.1155/2014/490589.

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Background. Tuberculosis (TB) is a chronic bacterial disease. Mycobacterium tuberculosis, being the leading member of the MTB complex, is the main cause of tuberculosis worldwide. Tuberculosis is managed with combination of drugs: streptomycin, isoniazid, rifampicin, ethambutol, and pyrazinamide. Over the recent past years resistance against first line antituberculous drugs has emerged rapidly throughout the world resulting in MDR strains. The new threat in the management of MDR-TB is the development of resistance against second line drugs: aminoglycosides, polypeptides, fluoroquinolones, and
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Peña, Jeremy A., Mary Jane Ferraro, Colleen G. Hoffman, and John A. Branda. "Growth Detection Failures by the Nonradiometric Bactec MGIT 960 Mycobacterial Culture System." Journal of Clinical Microbiology 50, no. 6 (2012): 2092–95. http://dx.doi.org/10.1128/jcm.00108-12.

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27

Heginbothom, M. L., P. G. Flanagan, and J. T. Magee. "Susceptibility testing of Mycobacterium tuberculosis with the BACTEC™ MGIT™ 960." Journal of Infection 40, no. 2 (2000): A16. http://dx.doi.org/10.1016/s0163-4453(00)80059-6.

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Chen, Junlin, Fei Huang, Feifan Xu, et al. "Rapid Detection of Mycobacterium Tuberculosis in Lung Tissue and Analysis of Drug Resistance." Nanoscience and Nanotechnology Letters 11, no. 5 (2019): 728–35. http://dx.doi.org/10.1166/nnl.2019.2939.

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This study investigated the methods for optimizing the workflow for improving the diagnostic efficiency for detection of Mycobacterium tuberculosis (MTB) in lung tissue specimens. A total of 278 specimens were used in this study. M. tuberculosis in fresh lung tissue samples was detected by BACTEC MGIT 960 culture system, culturing L-form MTB, rifampicin (RFP) and levofloxacin (LVFX) susceptibility test, and ribonucleic acid (RNA) simultaneous amplification and testing (SAT). Specimen samples were embedded in paraffin and serially sectioned. The sections were subjected to Ziehl-Neelsen staining
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Andres, Sönke, Doris Hillemann, Sabine Rüsch-Gerdes, and Elvira Richter. "Occurrence ofrpoBMutations in Isoniazid-Resistant but Rifampin-Susceptible Mycobacterium tuberculosis Isolates from Germany." Antimicrobial Agents and Chemotherapy 58, no. 1 (2013): 590–92. http://dx.doi.org/10.1128/aac.01752-13.

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ABSTRACTFour out of 143 phenotypically isoniazid-resistant but rifampin-susceptibleMycobacterium tuberculosisstrains that were isolated from patients in Germany in 2011 had mutations in the rifampin resistance-determining region ofrpoB. After performing drug susceptibility testing (DST) with two methods, the proportion method on Löwenstein-Jensen medium and using the Bactec 960 Mycobacteria Growth Indicator Tube system, we conclude that the two methods are equally reliable for phenotypic DST and MIC determination.
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Marwah, Mohamed, M. Moussa I, F. Mohamed Kh, et al. "BACTEC MGIT 960 TM system for screening of Mycobacterium tuberculosis complex among cattle." African Journal of Biotechnology 10, no. 63 (2011): 13919–23. http://dx.doi.org/10.5897/ajb11.1374.

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Piersimoni, C., D. Nista, S. Bornigia, and G. Gherardi. "Unreliable Detection of Mycobacterium xenopi by the Nonradiometric Bactec MGIT 960 Culture System." Journal of Clinical Microbiology 47, no. 3 (2009): 804–6. http://dx.doi.org/10.1128/jcm.01444-08.

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Ovsyankina, E. S., L. V. Panova, E. A. Krushinskaya, O. A. Piskunova, and F. G. Poluektova. "ON THE QUESTION OF REDUCED DURATION OF PULMONARY TB TREATMENT IN ADOLESCENTS WITH DRUG SUSCEPTIBILITY DETERMINATION DURING TREATMENT." Вестник ЦНИИТ 7, no. 2 (2023): 67–72. http://dx.doi.org/10.57014/2587-6678-2023-7-2-67-72.

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We carried out a cohort retrospective study to justify the possibilities to continue the main TB treatment course in adolescents with determined drug susceptibility during treatment (without return to treatment commencement) in order to reduce the total duration of treatment. Out of 123 patients who completed treatment in 2019–2022 we selected 31 patients with drug susceptibility test (DST) results obtained by only cultural studies using BACTEC MGIT 960 after 1.5-2 months of treatment. We established that DST results obtained using BACTEC MGIT 960 during treatment would determine further treat
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Nosova, E. Yu, M. A. Sviridenko, A. A. Khakhalina, K. Yu Galkina, Yu D. Mikhailova, and S. G. Safonova. "Rapid diagnostics of tuberculosis using the Xpert MTB/Rif Ultra test." Tuberculosis and socially significant diseases 13, no. 1 (2025): 22–29. https://doi.org/10.54921/2413-0346-2025-13-1-22-29.

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The aim of the study was evaluation of the diagnostic efficiency of the Xpert MTB/RIF Ultra test for detection of M. tuberculosis DNA and determination of drug susceptibility to rifampicin in various diagnostic samples of patients examined for disease verification.Materials and methods. We analyzed 427 respiratory and extrapulmonary diagnostic samples obtained from 378 patients (including HIV-infected ones) requiring rapid TB diagnostics. The study was conducted from one portion of the sediment of the diagnostic sample using bacteriological methods (luminescent microscopy, cultivation on Middl
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Mikhailova, Y. D., M. V. Makarova, L. Y. Krylova, et al. "Study of drug susceptibility of Mycobacterium tuberculosis to delamanid using BACTECTM MGITTM 960 automated system." Tuberculosis and socially significant diseases 12, no. 4 (2025): 25–35. https://doi.org/10.54921/2413-0346-2024-12-4-25-35.

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Aim. To study the susceptibility of clinical strains of M. tuberculosis complex (MTB) to delamanid using the automated Bactec MGIT system.Material and methods. We studied 79 M. tuberculosis isolates from 78 TB patients treated in 2017-2023. 39 MTB strains were susceptibile and 40 had different drug resistance profiles to anti-tubercular drugs. Minimum inhibitory concentrations (MIC) of delamanid in Middlebrook 7H9 liquid medium were determined using the Bactec MGIT system, the susceptibility results were assessed using the critical concentration of 0.06 μg/ml recommended by WHO. Whole genome s
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Muchwa, Christopher, Joseph Akol, Alfred Etwom, et al. "Evaluation of Capilia TB assay for rapid identification of Mycobacterium tuberculosis complex in BACTEC MGIT 960 and BACTEC 9120 blood cultures." BMC Research Notes 5, no. 1 (2012): 44. http://dx.doi.org/10.1186/1756-0500-5-44.

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Scarparo, Claudio, Paola Piccoli, Alessandra Rigon, Giuliana Ruggiero, Paolo Ricordi, and Claudio Piersimoni. "Evaluation of the BACTEC MGIT 960 in comparison with BACTEC 460 TB for detection and recovery of mycobacteria from clinical specimens." Diagnostic Microbiology and Infectious Disease 44, no. 2 (2002): 157–61. http://dx.doi.org/10.1016/s0732-8893(02)00437-6.

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37

Domotenko, L. V., T. P. Morozova, M. V. Khramov, and А. P. Shepelin. "Testing Susceptibility of M. tuberculosis to Second Line Anti-Tuberculosis Drugs Using the XDR Test in Clinical Trials and International Professional Testing Cycles." Tuberculosis and Lung Diseases 99, no. 8 (2021): 13–20. http://dx.doi.org/10.21292/2075-1230-2021-99-8-13-20.

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The objective of the study: to evaluate the commercial XDR test for susceptibility testing of M. tuberculosis to second line anti-tuberculosis drugs in clinical trials and as part of annual professional testing cycles coordinated by the World Health Organization (WHO).Subjects and Methods. Cultures of M. tuberculosis (n = 90) freshly isolated on egg media from clinical samples collected in tuberculosis patients were tested using the Bactec MGIT 960 system and the XDR test under identical conditions. Well-studied strains of M. tuberculosis (n = 216) obtained from the WHO supranational laborator
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38

Somoskövi, Ákos, Csaba Ködmön, Ákos Lantos, et al. "Comparison of Recoveries of Mycobacterium tuberculosis Using the Automated BACTEC MGIT 960 System, the BACTEC 460 TB System, and Löwenstein-Jensen Medium." Journal of Clinical Microbiology 38, no. 6 (2000): 2395–97. http://dx.doi.org/10.1128/jcm.38.6.2395-2397.2000.

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39

Somoskövi, Ákos, Csaba Ködmön, Ákos Lantos, et al. "Comparison of Recoveries of Mycobacterium tuberculosis Using the Automated BACTEC MGIT 960 System, the BACTEC 460 TB System, and Löwenstein-Jensen Medium." Journal of Clinical Microbiology 38, no. 6 (2000): 2395–97. http://dx.doi.org/10.1128/.38.6.2395-2397.2000.

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40

Hines, Nichole, Janet B. Payeur, and Lorraine J. Hoffman. "Comparison of the Recovery ofMycobacterium BovisIsolates Using the BACTEC MGIT 960 System, BACTEC 460 System, and Middlebrook 7H10 and 7H11 Solid Media." Journal of Veterinary Diagnostic Investigation 18, no. 3 (2006): 243–50. http://dx.doi.org/10.1177/104063870601800302.

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41

Mondal, Rajesh, Gomathi Sivaramakrishnan, Balaji Subramanyam, MichelPrem Kumar, Radhika Golla, and SrikanthPrasad Tripathy. "Validation of bedaquiline drug-susceptibility testing by BACTEC MGIT 960 system for Mycobacterium tuberculosis." International Journal of Mycobacteriology 8, no. 4 (2019): 329. http://dx.doi.org/10.4103/ijmy.ijmy_151_19.

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42

Kanchana, M. V., D. Cheke, I. Natyshak, B. Connor, A. Warner, and T. Martin. "Evaluation of the BACTEC™ MGIT™ 960 system for the recovery of mycobacteria." Diagnostic Microbiology and Infectious Disease 37, no. 1 (2000): 31–36. http://dx.doi.org/10.1016/s0732-8893(99)00151-0.

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43

Maslov, Dmitry A., Marina V. Zaĭchikova, Larisa N. Chernousova, et al. "Resistance to pyrazinamide in Russian Mycobacterium tuberculosis isolates: pncA sequencing versus Bactec MGIT 960." Tuberculosis 95, no. 5 (2015): 608–12. http://dx.doi.org/10.1016/j.tube.2015.05.013.

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44

Martin, Anandi, Krista Fissette, Francis Varaine, Françoise Portaels, and Juan Carlos Palomino. "Thin layer agar compared to BACTEC MGIT 960 for early detection of Mycobacterium tuberculosis." Journal of Microbiological Methods 78, no. 1 (2009): 107–8. http://dx.doi.org/10.1016/j.mimet.2009.05.001.

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Mustazzolu, Alessandro, Angelo Iacobino, Federico Giannoni, Claudio Piersimoni, and Lanfranco Fattorini. "Improved Bactec MGIT 960 Pyrazinamide Test Decreases Detection of False Mycobacterium tuberculosis Pyrazinamide Resistance." Journal of Clinical Microbiology 55, no. 12 (2017): 3552–53. http://dx.doi.org/10.1128/jcm.01437-17.

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46

Belyaev, D. V., T. V. Umpeleva, D. V. Dianov, L. S. Lavrenchuk, T. Yu Boteva, and D. V. Vakhrusheva. "Phenotypic Resistance to Rifampicin of Mycobacterium tuberculosis with the rpoB Leu430Pro Mutation." Tuberculosis and Lung Diseases 102, no. 5 (2024): 64–69. http://dx.doi.org/10.58838/2075-1230-2024-102-5-64-69.

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The objective: to determine the role of the rpoB Leu430Pro mutation in the degree of phenotypic resistance of Mycobacterium tuberculosis to rifampicin.Subjects and Methods. The WHO classifies the rpoB Leu430Pro mutation of Mycobacterium tuberculosis as a borderline resistance mutation but of clinical significance. From an array of cultures, we selected and analyzed 14 isolates of M. tuberculosis with discrepancies in the results of molecular genetic and phenotypic testing, carrying only this mutation in rpoB. For all samples, the phenotypic resistance level (MIC) of these isolates to rifampici
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47

Isakova, A. I., Yu D. Mikhailova, K. Yu Galkina, E. Yu Nosova, and S. G. Safonova. "Evaluation of the effectiveness of molecular genetic and bacteriological methods in determining heteroresistance Mycobacterium tuberculosis to rifampicin." Tuberculosis and socially significant diseases 10, no. 4 (2022): 32–41. http://dx.doi.org/10.54921/2413-0346-2022-10-4-48-57.

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We analyzed bacterial suspensions (mixtures) containing various proportions of resistant and susceptible strains to assess the effectiveness of molecular genetic tests and bacteriological methods for determination of the heteroresistant population of M. tuberculosis (MTB) to rifampicin (RIF). We established that the efficiency of determining the RIF-resistant MTB population in mixtures depends on the method of mutation detection used in the test system. The Genotype MTBDRplus, TB-TEST, and TB-BIOCHIP-1 tests showed the same results in detecting a resistant population with the Ser531Leu mutatio
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Novikova, T. A., N. L. Shaporova, T. V. Rubanik, N. A. Ilyina, and J. S. Cherkanova. "Features of the diagnosis and treatment of nontuberculous mycobacterial lung disease caused by M. avium and M. abscessus in outpatient settings (a clinical case)." New St. Petersburg Medical Records, no. 4 (March 19, 2025): 70–78. https://doi.org/10.24884/1609-2201-2024-103-4-70-78.

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A case study of a 71-year-old patient with non-tuberculous mycobacteriosis (NTML) of the lungs is presented, caused by a combination of two types of non-tuberculosis mycobacteria: M. avium and M. abscessus.The aim is to demonstrate the complexity of the diagnosis and therapy of NTML in outpatient settings, as well as the need for repeated cultural studies, species typing and determination of sensitivity to biomaterial drugs.Materials and methods: clinical and biochemical blood tests, general sputum and VC analysis, examination of the function of external respiration, computed tomography of the
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Myneedu, VP, R. Singhal, M. Bhalla, et al. "Presumptive identification of Mycobacterium tuberculosis complex based on cord formation in BACTEC MGIT 960 medium." Indian Journal of Medical Microbiology 30, no. 2 (2012): 218. http://dx.doi.org/10.4103/0255-0857.96697.

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Hasan, Mehedi, Saurab Kishore Munshi, Mst Sabiha Banu Momi, Farjana Rahman, and Rashed Noor. "Evaluation of the effectiveness of BACTEC MGIT 960 for the detection of mycobacteria in Bangladesh." International Journal of Mycobacteriology 2, no. 4 (2013): 214–19. http://dx.doi.org/10.1016/j.ijmyco.2013.09.001.

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