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Journal articles on the topic 'Bacteria; Bone'

Author: Grafiati

Published: 4 June 2021

Last updated: 9 February 2022

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1

Carpenter, Kenneth. "Experimental investigation of the role of bacteria in bone fossilization." Neues Jahrbuch für Geologie und Paläontologie - Monatshefte 2005, no. 2 (2005): 83–94. http://dx.doi.org/10.1127/njgpm/2005/2005/83.

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2

Gollwitzer, Hans, Wolfram Mittelmeier, Monika Brendle, et al. "High Hydrostatic Pressure for Disinfection of Bone Grafts and Biomaterials: An Experimental Study." Open Orthopaedics Journal 3, no. 1 (2009): 1–7. http://dx.doi.org/10.2174/1874325000903010001.

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Background:Autoclaving, heat, irradiation or chemical detergents are used to disinfect autografts, allografts and biomaterials for tissue reconstruction. These methods are often associated with deterioration of mechanical, physical, and biological properties of the bone grafts and synthetic implants. High hydrostatic pressure has been proposed as a novel method preserving biomechanical and biological properties of bone, tendon and cartilage. This is the first study to assess the inactivation of clinically relevant bacteria on biomaterials and human bone by high hydrostatic pressure.Methods:Bac

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3

Charles, Krista. "Bacteria from yogurt speed bone healing." New Scientist 248, no. 3309 (2020): 22. http://dx.doi.org/10.1016/s0262-4079(20)32038-8.

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Parvaneh, Kolsoom, Rosita Jamaluddin, Golgis Karimi, and Reza Erfani. "Effect of Probiotics Supplementation on Bone Mineral Content and Bone Mass Density." Scientific World Journal 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/595962.

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A few studies in animals and a study in humans showed a positive effect of probiotic on bone metabolism and bone mass density. Most of the investigated bacteria wereLactobacillusandBifidobacterium. The positive results of the probiotics were supported by the high content of dietary calcium and the high amounts of supplemented probiotics. Some of the principal mechanisms include (1) increasing mineral solubility due to production of short chain fatty acids; (2) producing phytase enzyme by bacteria to overcome the effect of mineral depressed by phytate; (3) reducing intestinal inflammation follo

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Procopio, Noemi, Caley A. Mein, Sefora Starace, Andrea Bonicelli, and Anna Williams. "Bone Diagenesis in Short Timescales: Insights from an Exploratory Proteomic Analysis." Biology 10, no. 6 (2021): 460. http://dx.doi.org/10.3390/biology10060460.

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The evaluation of bone diagenetic phenomena in archaeological timescales has a long history; however, little is known about the origins of the microbes driving bone diagenesis, nor about the extent of bone diagenesis in short timeframes—such as in forensic contexts. Previously, the analysis of non-collagenous proteins (NCPs) through bottom-up proteomics revealed the presence of potential biomarkers useful in estimating the post-mortem interval (PMI). However, there is still a great need for enhancing the understanding of the diagenetic processes taking place in forensic timeframes, and to clar

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Mousa, H. A. "Bone infection." Eastern Mediterranean Health Journal 9, no. 1-2 (2003): 208–14. http://dx.doi.org/10.26719/2003.9.1-2.208.

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Osteomyelitis, or bone infection, affects all age groups and develops from various sources including haematogenously from distant infection foci, from external sources such as post-operative or post-traumatic wound infections and from adjoining soft tissue infections. Staphylococcus aureus, Streptococcus pyogenes and Haemophilus influenzae are the most common pathogens of haematogenous osteomyelitis. Aerobic and facultative gram-negative bacteria have emerged as significant pathogens in some types of osteomyelitis while anaerobic bacteria are increasingly recognized as potential pathogens in n

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Ren, Xiao Qing, Peng Liang, Li Zhen Ma, and Hong Shun Yang. "Antibacterial Mechanism of Catfish Bone Hydrolysate Revealed by Atomic Force and Transmission Electron Microscopy." Advanced Materials Research 554-556 (July 2012): 1346–49. http://dx.doi.org/10.4028/www.scientific.net/amr.554-556.1346.

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The aim of this study was to investigate the effect of the catfish bone hydrolysate (CBH) on morphology of bacteria which were observed by atomic force microscope (AFM) and transmission electron microscope (TEM). The CBH was found to inhibit Escherichia coli (E. coli) growth. The CBH at 10 mg/ml caused the significant fragmentariness in the bacterial membrane and a severe volume decrease. A possible mechanism is that CBH damages the structure of bacterial cell membrane which causes E. coli bacteria to die eventually.

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Jabbour, Zaher, Cássio do Nascimento, Michel El-Hakim, Janet E. Henderson, and Rubens F. de Albuquerque. "Profile of bacteria colonizing the exposed bone of patients with anti-osteoclastic drug-related osteonecrosis of the jaws." Canadian Journal of Microbiology 62, no. 9 (2016): 772–80. http://dx.doi.org/10.1139/cjm-2016-0212.

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Microbial etiology for anti-osteoclastic drug-related osteonecrosis of the jaw (ARONJ) was suggested. This study investigates any link between bacteria colonizing ARONJ sites and other oral cavity sites. Microbiota samples of 10 ARONJ patients were collected from the exposed bone, adjacent teeth, contralateral teeth, and tongue. DNA checkerboard hybridization was used for microbiota analysis with 43 genomic DNA probes prepared from human oral bacterial (38) and candida (5) species, using Socransky’s bacterial complexes as a guide. The frequency and the mean proportion of each bacterial species

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9

Kolbjørnsen, Ø., B. David, and M. Gilhuus. "Bacterial Osteomyelitis in a 3-Week-Old Broiler Chicken Associated With Enterococcus hirae." Veterinary Pathology 48, no. 6 (2011): 1134–37. http://dx.doi.org/10.1177/0300985810396513.

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Enterococcus hirae infections are reported to cause growth depression, encephalomalacia, endocarditis, and septicemia in chickens. This report describes osteomyelitis in the proximal femur of a 3-week-old broiler chicken that also suffered from valvular endocarditis and liver necrosis. Histologically, clusters of gram-positive coccoid bacteria were found in many organs, including bone lesions. In tissues from 5 of 6 examined chickens from the same flock, E hirae was isolated in large numbers. To the authors' knowledge, this is the first report of spontaneous bacterial osteomyelitis where E hir

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Winkler, Heinz, and Peter Haiden. "Allograft Bone as Antibiotic Carrier." Journal of Bone and Joint Infection 2, no. 1 (2017): 52–62. http://dx.doi.org/10.7150/jbji.17466.

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Abstract. The treatment of chronic bone and joint infections is characterized by obstinate persistency of the causing microorganisms and resulting long term disability to patients, associated with remarkable costs for the health care system. Difficulties derive from biofilm formed on dead bone and eventual implants, with resistance against immunological defences and antimicrobial substances. Biofilm embedded bacteria require up to 1000 times the antibiotic concentration of planktonic bacteria for elimination. Systemic antibiotic treatment alone cannot provide the concentrations required and su

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11

Kwon, Yeongkag, Chaeyeon Park, Jueun Lee, et al. "Regulation of Bone Cell Differentiation and Activation by Microbe-Associated Molecular Patterns." International Journal of Molecular Sciences 22, no. 11 (2021): 5805. http://dx.doi.org/10.3390/ijms22115805.

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Gut microbiota has emerged as an important regulator of bone homeostasis. In particular, the modulation of innate immunity and bone homeostasis is mediated through the interaction between microbe-associated molecular patterns (MAMPs) and the host pattern recognition receptors including Toll-like receptors and nucleotide-binding oligomerization domains. Pathogenic bacteria such as Porphyromonas gingivalis and Staphylococcus aureus tend to induce bone destruction and cause various inflammatory bone diseases including periodontal diseases, osteomyelitis, and septic arthritis. On the other hand, p

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Dapunt, Ulrike, Susanne Maurer, Thomas Giese, Matthias Martin Gaida та Gertrud Maria Hänsch. "The Macrophage Inflammatory Proteins MIP1α(CCL3) and MIP2α(CXCL2) in Implant-Associated Osteomyelitis: Linking Inflammation to Bone Degradation". Mediators of Inflammation 2014 (2014): 1–10. http://dx.doi.org/10.1155/2014/728619.

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Bacterial infections of bones remain a serious complication of endoprosthetic surgery. These infections are difficult to treat, because many bacterial species form biofilms on implants, which are relatively resistant towards antibiotics. Bacterial biofilms elicit a progressive local inflammatory response, resulting in tissue damage and bone degradation. In the majority of patients, replacement of the prosthesis is required. To address the question of how the local inflammatory response is linked to bone degradation, tissue samples were taken during surgery and gene expression of the macrophage

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Yagi, Haruyo, Shinsuke Kihara, Peter N. Mittwede, et al. "Development of a large animal rabbit model for chronic periprosthetic joint infection." Bone & Joint Research 10, no. 3 (2021): 156–65. http://dx.doi.org/10.1302/2046-3758.103.bjr-2019-0193.r3.

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Aims Periprosthetic joint infections (PJIs) and osteomyelitis are clinical challenges that are difficult to eradicate. Well-characterized large animal models necessary for testing and validating new treatment strategies for these conditions are lacking. The purpose of this study was to develop a rabbit model of chronic PJI in the distal femur. Methods Fresh suspensions of Staphylococcus aureus (ATCC 25923) were prepared in phosphate-buffered saline (PBS) (1 × 109 colony-forming units (CFUs)/ml). Periprosthetic osteomyelitis in female New Zealand white rabbits was induced by intraosseous inject

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De Sousa, Ricardo Barbosa, Ewerton Gomes Vieira, Andréia Bagliotti Meneguin, Rafael Miguel Sábio, Josy Anteveli Osajima Furtini, and Edson Cavalcanti Da Silva Filho. "Recent advances in methods of synthesis and applications of bacterial cellulose/calcium phosphates composites in bone tissue engineering." International Journal of Advances in Medical Biotechnology - IJAMB 1, no. 2 (2018): 11. http://dx.doi.org/10.25061/2595-3931/ijamb/2018.v1i2.16.

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Bacterial cellulose (BC) is a nanofibrous biomaterial biosynthetized by a series of acetic bacteria with unique properties with application in many tissue engineering purposes. Calcium phosphates (CPs), mainly hydroxyapatite, are bioceramics that possess similar composition of host bones and are able to stimulate osteoconduction and osteointegration to living tissues. Bacterial cellulose-calcium phosphates composites have caught the attention of researchers by their excellent mechanical properties and biocompatibility, being considered an excellent proposal to development of new synthetic graf

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15

Stracquadanio, Stefano, Nicolò Musso, Angelita Costantino, Lorenzo Mattia Lazzaro, Stefania Stefani, and Dafne Bongiorno. "Staphylococcus aureus Internalization in Osteoblast Cells: Mechanisms, Interactions and Biochemical Processes. What Did We Learn from Experimental Models?" Pathogens 10, no. 2 (2021): 239. http://dx.doi.org/10.3390/pathogens10020239.

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Bacterial internalization is a strategy that non-intracellular microorganisms use to escape the host immune system and survive inside the human body. Among bacterial species, Staphylococcus aureus showed the ability to interact with and infect osteoblasts, causing osteomyelitis as well as bone and joint infection, while also becoming increasingly resistant to antibiotic therapy and a reservoir of bacteria that can make the infection difficult to cure. Despite being a serious issue in orthopedic surgery, little is known about the mechanisms that allow bacteria to enter and survive inside the os

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Zapata, Mayra Eliana Valencia, Lina Marcela Ruiz Rojas, José Herminsul Mina Hernández, Johannes Delgado-Ospina, and Carlos David Grande Tovar. "Acrylic Bone Cements Modified with Graphene Oxide: Mechanical, Physical, and Antibacterial Properties." Polymers 12, no. 8 (2020): 1773. http://dx.doi.org/10.3390/polym12081773.

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Bacterial infections are a common complication after total joint replacements (TJRs), the treatment of which is usually based on the application of antibiotic-loaded cements; however, owing to the increase in antibiotic-resistant microorganisms, the possibility of studying new antibacterial agents in acrylic bone cements (ABCs) is open. In this study, the antibacterial effect of formulations of ABCs loaded with graphene oxide (GO) between 0 and 0.5 wt.% was evaluated against Gram-positive bacteria: Bacillus cereus and Staphylococcus aureus, and Gram-negative ones: Salmonella enterica and Esche

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Honda, Michiyo, Morio Matsumoto, and Mamoru Aizawa. "Potential Application of Protamine for Antimicrobial Biomaterials in Bone Tissue Engineering." International Journal of Molecular Sciences 21, no. 12 (2020): 4368. http://dx.doi.org/10.3390/ijms21124368.

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Bacterial infection of biomaterials is a serious problem in the field of medical devices. It is urgently necessary to develop new biomaterials with bactericidal activity. Antimicrobial peptides and proteins (AMPs), alternative antibacterial agents, are expected to overcome the bacterial resistance. The aim of this study was to develop a new intelligent material in bone tissue engineering based on protamine-loaded hydroxyapatite (protamine/HAp) that uses AMPs rather than antibiotics. It was found that the adsorption of protamine to HAp followed the Langmuir adsorption model and was due to elect

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18

Zink, Albert, Udo Reischl, Hans Wolf, and Andreas G. Nerlich. "Molecular Evidence of Bacteremia by Gastrointestinal Pathogenic Bacteria in an Infant Mummy From Ancient Egypt." Archives of Pathology & Laboratory Medicine 124, no. 11 (2000): 1614–18. http://dx.doi.org/10.5858/2000-124-1614-meobbg.

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Abstract In this study, we describe an infant mummy from ancient Egypt that showed macromorphologic signs of chronic anemia and vitamin C deficiency. From this infant, we have obtained a sterile sample from a metatarsal bone to extract ancient bacterial DNA. Following polymerase chain reaction amplification and subcloning of the amplicons, the sequence of the 16S ribosomal DNA was determined in several resulting clones. The presence of pathogenic and apathogenic bacteria, such as Escherichia coli, are indicated by our result, providing evidence of bacteremia, which probably contributed to deat

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19

Willekens, Christophe, and Thomas Boyer. "Bone marrow necrosis: a culture medium for bacteria." Blood 122, no. 16 (2013): 2775. http://dx.doi.org/10.1182/blood-2013-06-505677.

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20

Chen, Mei-Feng, Chih-Hsiang Chang, Chih-Chien Hu, Ying-Yu Wu, Yuhan Chang, and Steve W. N. Ueng. "Periprosthetic Joint Infection Caused by Gram-Positive Versus Gram-Negative Bacteria: Lipopolysaccharide, but not Lipoteichoic Acid, Exerts Adverse Osteoclast-Mediated Effects on the Bone." Journal of Clinical Medicine 8, no. 9 (2019): 1289. http://dx.doi.org/10.3390/jcm8091289.

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Periprosthetic joint infection (PJI)—the most common cause of knee arthroplasty failure—may result from Gram-positive (GP) or Gram-negative (GN) bacterial infections. The question as to whether PJI due to GP or GN bacteria can lead to different rates of aseptic loosening after reimplantation remains open. We have investigated this issue through a retrospective review of clinical records obtained from 320 patients with bacterial PJI. The results revealed that, compared with GP infections, GN infections were associated with an increased risk of aseptic loosening. In animal studies, mice underwen

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21

Cardoso, Flávia Goulart da Rosa, Adriana Chung, Frederico Canato Martinho, et al. "Investigation of Bacterial Contents From Persistent Endodontic Infection and Evaluation of Their Inflammatory Potential." Brazilian Dental Journal 27, no. 4 (2016): 412–18. http://dx.doi.org/10.1590/0103-6440201600520.

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Abstract This clinical study investigated and quantified cultivable bacteria and their levels of endotoxins in persistent endodontic infection, determining their antigenicity against macrophages and fibroblast cells by IL-1β and TNF-α secretion and evaluating their relationship with clinical and radiographic features. Samples from the root canals were obtained after root filling removal. Culture techniques were used to determine the bacterial count and the endotoxins were determined by LAL-assay. PCR analysis (16S rDNA) was used for bacterial detection. Raw 264.5 macrophages and V79 fibroblast

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Pöllänen, M. T., M. A. Laine, R. Ihalin, and V. J. Uitto. "Host-Bacteria Crosstalk at the Dentogingival Junction." International Journal of Dentistry 2012 (2012): 1–14. http://dx.doi.org/10.1155/2012/821383.

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The dentogingival junction is of crucial importance in periodontal host defense both structurally and functionally. Oral bacteria exert a constant challenge to the host cells and tissues at the dentogingival junction. The host response is set up to eliminate the pathogens by the innate and adaptive defense mechanisms. In health, the commensal bacteria and the host defense mechanisms are in a dynamic steady state. During periodontal disease progression, the dental bacterial plaque, junctional epithelium (JE), inflammatory cells, connective tissue, and bone all go through a series of changes. Th

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LIANG, RONGRONG, XIAOQIAO YU, RENHUAN WANG, et al. "Bacterial Diversity and Spoilage-Related Microbiota Associated with Freshly Prepared Chicken Products under Aerobic Conditions at 4°C." Journal of Food Protection 75, no. 6 (2012): 1057–62. http://dx.doi.org/10.4315/0362-028x.jfp-11-439.

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This study analyzed the bacterial diversity and spoilage-related microbiota associated with freshly prepared chicken products stored aerobically at 4°C, using “bone and chicken string,” a product popular in the People's Republic of China, as the study subject. Samples collected from three different factories were tray packaged with cling film and stored at 4°C. Bacterial diversity and dominant bacteria were analyzed using PCR amplification and denaturing gradient gel electrophoresis. Combined with selective cultivation of the dominant bacteria and correlation analysis, the dominant spoilage mi

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Holloway, Julianne L. "One step solution for fighting bacteria and growing bone." Science Translational Medicine 11, no. 479 (2019): eaaw5326. http://dx.doi.org/10.1126/scitranslmed.aaw5326.

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The delivery of copper ions from collagen-bioactive glass composite scaffolds offers a promising one-step approach to treating bone infections while also promoting new bone and blood vessel formation.

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Marriott, Ian, Dana M. Rati, Samuel H. McCall, and Susanne L. Tranguch. "Induction of Nod1 and Nod2 Intracellular Pattern Recognition Receptors in Murine Osteoblasts following Bacterial Challenge." Infection and Immunity 73, no. 5 (2005): 2967–73. http://dx.doi.org/10.1128/iai.73.5.2967-2973.2005.

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ABSTRACT Osteoblasts produce an array of immune molecules following bacterial challenge that could recruit leukocytes to sites of infection and promote inflammation during bone diseases, such as osteomyelitis. Recent studies from our laboratory have shed light on the mechanisms by which this cell type can perceive and respond to bacteria by demonstrating the functional expression of members of the Toll-like family of cell surface pattern recognition receptors by osteoblasts. However, we have shown that bacterial components fail to elicit immune responses comparable with those seen following ch

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Mongaret, Céline, Jennifer Varin-Simon, Fabien Lamret, et al. "Cutibacterium acnes Biofilm Study during Bone Cells Interaction." Microorganisms 8, no. 9 (2020): 1409. http://dx.doi.org/10.3390/microorganisms8091409.

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Cutibacterium acnes is an opportunistic pathogen involved in Bone and Prosthesis Infections (BPIs). In this study, we observed the behavior of commensal and BPI C. acnes strains in the bone environment through bacterial internalization by osteoblast-like cells and biofilm formation. For the commensal strains, less than 1% of the bacteria were internalized; among them, about 32.7 ± 3.9% persisted intracellularly for up to 48 h. C. acnes infection seems to have no cytotoxic effect on bone cells as detected by LDH assay. Interestingly, commensal C. acnes showed a significant increase in biofilm f

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Rahyussalim, Ahmad Jabir, Tri Kurniawati, and Andriansjah Rukmana. "Mycobacterium tuberculosisContaminant Risk on Bone Marrow Aspiration Material from Iliac Bone Patients with Active Tuberculous Spondylitis." BioMed Research International 2016 (2016): 1–5. http://dx.doi.org/10.1155/2016/3852940.

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There was a concern onMycobacterium tuberculosisspreading to the bone marrow, when it was applied on tuberculous spine infection. This research aimed to study the probability of using autologous bone marrow as a source of mesenchymal stem cell for patients with tuberculous spondylitis. As many as nine patients with tuberculous spondylitis were used as samples. During the procedure, the vertebral lesion material and iliac bone marrow aspirates were obtained for acid fast staining, bacteria culture, and PCR (polymerase chain reaction) tests forMycobacterium tuberculosisat the Clinical Microbiolo

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Flanagan, Dennis. "Implant Placement in Failed Endodontic Sites: A Review." Journal of Oral Implantology 42, no. 2 (2016): 224–30. http://dx.doi.org/10.1563/aaid-joi-d-15-00126.

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Dental implants may fail to osseointegrate in sites of endodontic failure. This may occur as a result colonization by various anaerobic and facultative bacterial species. If an implant is placed in a site where vegetative bacteria are residing, the implant may fail to integrate if a bacterial colonization proceeds coronally. If the implant apical cortical bone is thin or if there is an apical fenestration, the colonization may proceed through the thin or nonexistent bone through the covering mucosa, relieving inflammatory pressure to create an apical (retrograde) peri-implantitis. Enterococcus

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Richardson, EF, and NO Brown. "Hematological and biochemical changes and results of aerobic bacteriological culturing in dogs undergoing splenectomy." Journal of the American Animal Hospital Association 32, no. 3 (1996): 199–210. http://dx.doi.org/10.5326/15473317-32-3-199.

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Changes in complete blood count (CBC), bone marrow, reticulocyte count, coagulation profile, biochemical analysis, and serum iron, transferrin, and immunoglobulin M (IgM) concentrations were measured three and 10 days after splenectomy in 12 dogs. Spleens were cultured aerobically for bacteria and submitted for histopathological evaluation in 23 dogs undergoing splenectomy. There were no consistent changes in red blood cell (RBC), white blood cell (WBC), or platelet counts; bone-marrow samples; or biochemical profiles. Serum iron, transferrin, and IgM concentrations remained normal. Eight (35%

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Binte Atique, Fahmida, and Md Masudur Rahman Khalil. "The Bacterial Contamination of Allogeneic Bone and Emergence of Multidrug-Resistant Bacteria in Tissue Bank." BioMed Research International 2014 (2014): 1–5. http://dx.doi.org/10.1155/2014/430581.

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Present study was carried out for the microbiological evaluation of allogeneic bone processed from femoral heads. A total 60 bacterial isolates comprising five different species includingStreptococcusspp.,Staphylococcusspp.,Klebsiellaspp.,Bacillusspp., andPseudomonasspp. were characterized based on their cultural and biochemical characteristics. Average bioburden was ranged from5.7×101to3.9×104 cfu/gm. The majority (81.7%) of the microbial contaminants were detected as Gram positive with the predominant organism being skin commensal coagulase negative Staphylococci (43.3%). Antimicrobial resis

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Terada, Chika, Takahiro Imamura, Tomoko Ohshima, et al. "The Effect of Irradiation with a 405 nm Blue-Violet Laser on the Bacterial Adhesion on the Osteosynthetic Biomaterials." International Journal of Photoenergy 2018 (November 13, 2018): 1–10. http://dx.doi.org/10.1155/2018/2635964.

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Delayed postoperative infection is known as a major complication after bone surgeries using osteosynthetic biomaterial such as titanium (Ti) and bioresorbable organic materials. However, the precise cause of this type of infection is still unclear and no effective prevention has been established. The purpose of this study is to investigate the effect of irradiation with a 405 nm blue-violet laser on the bacteria adhered on the Ti and hydroxyapatite-poly-L-lactic acid- (HA-PLLA) based material surfaces and to verify the possibility of its clinical application to prevent the delayed postoperativ

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Naznin Akhtar, Naznin Akhtar. "Radiation Response of Bacteria Associated with Human Cancellous Bone." IOSR Journal of Pharmacy and Biological Sciences 6, no. 2 (2013): 79–84. http://dx.doi.org/10.9790/3008-627984.

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Novak, K. F., M. Govindaswami, J. L. Ebersole, W. Schaden, N. House, and M. J. Novak. "Effects of Low-energy Shock Waves on Oral Bacteria." Journal of Dental Research 87, no. 10 (2008): 928–31. http://dx.doi.org/10.1177/154405910808701009.

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We have recently demonstrated that extracorporeal shock-wave therapy (ESWT) is effective in promoting the healing of dermal wounds and in regenerating alveolar bone lost through periodontal disease. The objective of the present study was to determine any antibacterial effect of ESWT on oral bacteria. Monoculture suspensions of 6 bacterial species were treated with 100 to 500 pulses of ESWT at energy flux densities (EFD) of 0.12 mJ/mm2, 0.22 mJ/mm2, and 0.3 mJ/mm2. Following treatment, aliquots were plated for viability determination and compared with untreated controls. ESWT showed a significa

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Mariaux, Sandrine, Ulrika Furustrand Tafin, and Olivier Borens. "Diagnosis Of Persistent Infection In Prosthetic Two-Stage Exchange: PCR analysis of Sonication fluid From Bone Cement Spacers." Journal of Bone and Joint Infection 2, no. 4 (2017): 218–23. http://dx.doi.org/10.7150/jbji.23078.

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Abstract. Introduction: When treating periprosthetic joint infections with a two-stage procedure, antibiotic-impregnated spacers are used in the interval between removal of prosthesis and reimplantation. According to our experience, cultures of sonicated spacers are most often negative. The objective of our study was to investigate whether PCR analysis would improve the detection of bacteria in the spacer sonication fluid.Methods: A prospective monocentric study was performed from September 2014 to January 2016. Inclusion criteria were two-stage procedure for prosthetic infection and agreement

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Pandelaki, Elmi C. J., Audy D. Wuntu, and Henry F. Aritonang. "Aktivitas Antibakteri Komposit Ag – Tulang Ikan Cakalang pada Staphylococcus aureus." Jurnal MIPA 7, no. 2 (2018): 29. http://dx.doi.org/10.35799/jm.7.2.2018.21436.

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Telah dilakukan penelitian untuk mengetahui aktivitas antibakteri komposit Ag – tulang ikan cakalang pada Staphylococcus aureus. Tulang ikan cakalang dikeringkan, dihaluskan dan diayak 65 mesh kemudian dicampur dengan larutan perak nitrat dengan perbandingan Ag : tulang ikan sebesar 5:1 , 4:2, dan 3:3 selama 1 jam pada suhu 70 ℃. Campuran kemudian di kalsinasi pada suhu 650 ℃ selama 2 jam. Uji aktivitas antibakteri dari komposit yang terbentuk dikerjakan dengan metode sumuran menggunakan bakteri Staphylococcus aureus. Hasil penelitian menunjukkan aktivitas antibakteri pada perbandingan 4:2 den

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Jiang, Yanling, Chetan K. Mehta, Tun-Yi Hsu, and Fahad F. H. Alsulaimani. "Bacteria Induce Osteoclastogenesis via an Osteoblast-Independent Pathway." Infection and Immunity 70, no. 6 (2002): 3143–48. http://dx.doi.org/10.1128/iai.70.6.3143-3148.2002.

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ABSTRACT Bacteria or their products may cause chronic inflammation and subsequent bone loss. This inflammation and bone loss may be associated with significant morbidity in chronic otitis media, periodontitis, endodontic lesions, and loosening of orthopedic implants caused by lipopolysaccharide (LPS)-contaminated implant particles. Currently, it is not clear how bacteria or endotoxin-induced bone resorption occurs and what cell types are involved. Here we report that Porphyromonas gingivalis, a periodontal pathogen, and Escherichia coli LPS induce osteoclastic cell formation from murine leukoc

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Dauben, Thomas Josef, Josefin Ziebart, Thomas Bender, Sarah Zaatreh, Bernd Kreikemeyer, and Rainer Bader. "A Novel In Vitro System for Comparative Analyses of Bone Cells and Bacteria under Electrical Stimulation." BioMed Research International 2016 (2016): 1–12. http://dx.doi.org/10.1155/2016/5178640.

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Electrical stimulation is a promising approach to enhance bone regeneration while having potential to inhibit bacterial growth. To investigate effects of alternating electric field stimulation on both human osteoblasts and bacteria, a novel in vitro system was designed. Electric field distribution was simulated numerically and proved by experimental validation. Cells were stimulated on Ti6Al4V electrodes and in short distance to electrodes. Bacterial growth was enumerated in supernatant and on the electrode surface and biofilm formation was quantified. Electrical stimulation modulated gene exp

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Navarini, Alexander A., Karl S. Lang, Admar Verschoor, et al. "Innate immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections." Proceedings of the National Academy of Sciences 106, no. 17 (2009): 7107–12. http://dx.doi.org/10.1073/pnas.0901162106.

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Neutrophils are the most abundant leukocytes in circulation and provide a primary innate immune defense function against bacterial pathogens before development of a specific immune response. These specialized phagocytes are short lived (12–24 hours) and continuously replenished from bone marrow. We found that if the host is overwhelmed by a high inoculum ofListeria monocytogenes,neutrophils are depleted despite high granulocyte-colony stimulating factor induction. In contrast to a low-dose innocuousL. monocytogenesinfection, high-dose Listeria challenge blocks neutrophil recruitment to infecti

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Civantos, Ana, Ana M. Beltrán, Cristina Domínguez-Trujillo, et al. "Balancing Porosity and Mechanical Properties of Titanium Samples to Favor Cellular Growth against Bacteria." Metals 9, no. 10 (2019): 1039. http://dx.doi.org/10.3390/met9101039.

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Two main problems limit the success of titanium implants: bacterial infection, which restricts their osseointegration capacity; and the stiffness mismatch between the implant and the host cortical bone, which promotes bone resorption and risk of fracture. Porosity incorporation may reduce this difference in stiffness but compromise biomechanical behavior. In this work, the relationship between the microstructure (content, size, and shape of pores) and the antibacterial and cellular behavior of samples fabricated by the space-holder technique (50 vol % NH4HCO3 and three ranges of particle sizes

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Madrazo, Denise R., Susanne L. Tranguch, and Ian Marriott. "Signaling via Toll-Like Receptor 5 Can Initiate Inflammatory Mediator Production by Murine Osteoblasts." Infection and Immunity 71, no. 9 (2003): 5418–21. http://dx.doi.org/10.1128/iai.71.9.5418-5421.2003.

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ABSTRACT Murine osteoblasts express Toll-like receptor 5 (TLR5), and this expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Importantly, flagellin activates transcriptional regulators and elicits proinflammatory cytokine production, suggesting TLR5 functionality. TLR5 may represent an important mechanism underlying the recognition of bacterial pathogens by osteoblasts during bone infections.

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John, Minnie, Aseel Al-Jadiri, Christabelle Co, Maher Abulfaraj, and Lucia J. Santiago. "Acute Hematogenous Osteomyelitis in a Five-Month-Old Male with Rickets." Case Reports in Pediatrics 2017 (2017): 1–4. http://dx.doi.org/10.1155/2017/4627905.

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Osteomyelitis is defined as an infection of the bone, bone marrow, and the surrounding soft tissues. Most cases of acute hematogenous osteomyelitis in children are caused by Gram-positive bacteria, principally Staphylococcus aureus. We present a case where a 5-month-old male had an acute onset of decreased movement of his left leg and increased irritability and was subsequently diagnosed with rickets and hematogenous osteomyelitis with bacteremia. The case explores a possible association between hematogenous osteomyelitis and rickets.

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Eick, Sigrun, Kevin Hofpeter, Anton Sculean, et al. "Activity of Fosfomycin- and Daptomycin-Containing Bone Cement on Selected Bacterial Species Being Associated with Orthopedic Infections." BioMed Research International 2017 (2017): 1–13. http://dx.doi.org/10.1155/2017/2318174.

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The purpose of this study was to determine activity of fosfomycin/gentamicin and daptomycin/gentamicin-containing PMMA bone-cement against Staphylococcus aureus (MRSA, MSSA), Staphylococcus epidermidis, Enterococcus faecium (VRE), and E. coli (ESBL; only fosfomycin). Test specimens of the bone cement were formed and bacteria in two concentrations were added one time or repeatedly up to 96 h. All fosfomycin-containing cement killed ultimately all MSSA, Staphylococcus epidermidis, and E. coli within 24 h; growth of MRSA was suppressed up to 48 h. Activity of daptomycin-containing cement depended

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Chumsaengsri, Chumsaeng, Parichat Salee, and Worawit Louthrenoo. "Clinical Features and Treatment Outcomes of Bone-Joint Infection Between Bacteria and Mycobacterium Tuberculosis." Open Forum Infectious Diseases 4, suppl_1 (2017): S102. http://dx.doi.org/10.1093/ofid/ofx163.088.

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Abstract Background Bone-joint infection is an emergency condition that requires immediate management. Delayed in treatment or improper management can lead to a significant morbidity and mortality. Methods The medical records of patients with bone-joint infection seen at Maharaj Nakorn Chiang Mai Hospital between 1 November 2010 and 30 September 2015 were reviewed. The diagnosis of bone-joint infection was confirmed by pathogen identification or pathohistological report. Only those with adequate clinical features and treatment outcomes were included for analysis. Results Of 125 bone-joint infe

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Świątkiewicz, S., A. Arczewska-Włosek, and D. Józefiak. "Bones quality indices in laying hens fed diets with a high level of DDGS and supplemented with selected feed additives." Czech Journal of Animal Science 59, No. 2 (2014): 61–68. http://dx.doi.org/10.17221/7230-cjas.

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An experiment with 192 caged ISA Brown laying hens, fed a diet containing a high level of corn distillers dried grains with solubles (DDGS), was conducted to determine the influence of selected feed additives on biomechanical and geometrical indices of tibia and femur bones. At 26 weeks of age hens were randomly assigned to 8 treatments with 12 replicates (cages of two hens). To week 55, hens were fed isocaloric and isonitrogenous experimental diets either containing or not containing a high level of DDGS (200 g/kg). The diet containing 200 g/kg of DDGS was supplemented or not supplemented wit

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Holler, Ernst, Peter Oefner, Karin Landfried, et al. "Intestinal Microbiota: From Inflammatory Bowel Disease to Bone Marrow Transplantation." Blood 120, no. 21 (2012): SCI—50—SCI—50. http://dx.doi.org/10.1182/blood.v120.21.sci-50.sci-50.

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Abstract Abstract SCI-50 A dysregulated interaction of the intestinal microbiome with the patient's innate and adaptive immune response seems to contribute to both inflammatory bowel disease (IBD) and intestinal graft-versus-host disease (GVHD). In IBD, polymorphisms within genes involved in antibacterial defense have been identified as genetic risk factors, such as in NOD2, a gene coding for an intracytoplasmatic receptor for muramyldipeptide, a bacterial cell wall compound, or in ATG16L1, a gene involved in autophagy of bacteria. Disruption of these genes results in dysfunction of Paneth cel

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Badha, Vajra, Rex Moore, John Heffernan, Paulo Castaneda, Alex McLaren, and Derek Overstreet. "Determination of Tobramycin and Vancomycin Exposure Required to Eradicate Biofilms on Muscle and Bone Tissue In Vitro." Journal of Bone and Joint Infection 4, no. 1 (2019): 1–9. http://dx.doi.org/10.7150/jbji.29711.

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Abstract. Background: Bacterial biofilms cause chronic orthopaedic infections. Surgical debridement to remove biofilm can be ineffective without adjuvant local antimicrobials because undetected biofilm fragments may remain in the wound and reestablish the infection if untreated. However, the concentrations and duration of antimicrobial exposure necessary to eradicate bacteria from clinical biofilms remain largely undefined. In this study, we determined the minimum biofilm eradication concentration (MBEC) of tobramycin and vancomycin for bacterial biofilms grown on bone and muscle in vitro.Meth

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Bamashmous, Shatha, Georgios A. Kotsakis, Kristopher A. Kerns, et al. "Human variation in gingival inflammation." Proceedings of the National Academy of Sciences 118, no. 27 (2021): e2012578118. http://dx.doi.org/10.1073/pnas.2012578118.

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Oral commensal bacteria actively participate with gingival tissue to maintain healthy neutrophil surveillance and normal tissue and bone turnover processes. Disruption of this homeostatic host–bacteria relationship occurs during experimental gingivitis studies where it has been clearly established that increases in the bacterial burden increase gingival inflammation. Here, we show that experimental gingivitis resulted in three unique clinical inflammatory phenotypes (high, low, and slow) and reveal that interleukin-1β, a reported major gingivitis-associated inflammatory mediator, was not assoc

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Cortés-Vieyra, Ricarda, Carlos Rosales, and Eileen Uribe-Querol. "Neutrophil Functions in Periodontal Homeostasis." Journal of Immunology Research 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/1396106.

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Oral tissues are constantly exposed to damage from the mechanical effort of eating and to microorganisms, mostly bacteria. In healthy gingiva tissue remodeling and a balance between bacteria and innate immune cells are maintained. However, excess of bacteria biofilm (plaque) creates an inflammation state that recruits more immune cells, mainly neutrophils to the gingiva. Neutrophils create a barrier for bacteria to reach inside tissues. When neutrophils are insufficient, bacteria thrive causing more inflammation that has been associated with systemic effects on other conditions such as atheros

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Réglier-Poupet, Hélène, Elisabeth Pellegrini, Alain Charbit, and Patrick Berche. "Identification of LpeA, a PsaA-Like Membrane Protein That Promotes Cell Entry by Listeria monocytogenes." Infection and Immunity 71, no. 1 (2003): 474–82. http://dx.doi.org/10.1128/iai.71.1.474-482.2003.

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ABSTRACT The intracellular life of Listeria monocytogenes starts by a complex process of entry involving several bacterial ligands and eukaryotic receptors. In this work, we identified in silico from the sequence of the genome of L. monocytogenes a previously unknown gene designated lpeA (for lipoprotein promoting entry) encoding a 35-kDa protein homologous to PsaA, a lipoprotein belonging to the LraI family and implicated in the cell adherence of Streptococcus pneumoniae and related species. By constructing a mutant of L. monocytogenes in which lpeA is deleted (lpeA mutant), we show that the

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Lerner, Ulf H., G??ran Sundqvist, Acke Ohlin, and Jan B. Rosenquist. "Bacteria Inhibit Biosynthesis of Bone Matrix Proteins in Human Osteoblasts." Clinical Orthopaedics and Related Research 346 (January 1998): 244???254. http://dx.doi.org/10.1097/00003086-199801000-00032.

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