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Relevant bibliographies by topics / Bacteria transformed

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Academic literature on the topic 'Bacteria transformed'

Author: Grafiati

Published: 24 April 2022

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Journal articles on the topic "Bacteria transformed"

1

Reed, M. L. E., and Bernard R. Glick. "Growth of canola (Brassica napus) in the presence of plant growth-promoting bacteria and either copper or polycyclic aromatic hydrocarbons." Canadian Journal of Microbiology 51, no. 12 (2005): 1061–69. http://dx.doi.org/10.1139/w05-094.

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Growth of canola (Brassica napus) seeds treated with plant growth-promoting bacteria in copper-contaminated and polycyclic aromatic hydrocarbon (PAH)-contaminated soils was monitored. Pseudomonas asplenii AC, isolated from PAH-contaminated soil, was transformed to express a bacterial gene encoding 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and both native and transformed bacteria were tested for growth promotion. Inoculation of seeds, grown in the presence of copper or creosote, with either native or transformed P. asplenii AC significantly increased root and shoot biomass. Native and

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Nie, Xuan, Fan Gao, Fei Wang, Cheng Liu, and Ye-Zi You. "Charge-reversal silver clusters for targeted bacterial killing." Journal of Materials Chemistry B 9, no. 19 (2021): 4006–14. http://dx.doi.org/10.1039/d1tb00378j.

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Ag(−)/Ag(+) clusters with negatively charged nanoparticles and big-size are transformed into positively charged nanoparticles with small size in a bacterial acidic environment to kill the bacteria effectively.

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Buwembo, W., S. Aery, C. M. Rwenyonyi, G. Swedberg, and F. Kironde. "Point Mutations in thefolPGene Partly Explain Sulfonamide Resistance ofStreptococcus mutans." International Journal of Microbiology 2013 (2013): 1–6. http://dx.doi.org/10.1155/2013/367021.

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Cotrimoxazole inhibits dhfr and dhps and reportedly selects for drug resistance in pathogens. Here,Streptococcus mutansisolates were obtained from saliva of HIV/AIDS patients taking cotrimoxazole prophylaxis in Uganda. The isolates were tested for resistance to cotrimoxazole and theirfolPDNA (which encodes sulfonamide-targeted enzyme dhps) cloned in pUC19. A set of recombinant plasmids carrying different point mutations in cloned folP were separately transformed intofolP-deficientEscherichia coli. Using sulfonamide-containing media, we assessed the growth offolP-deficient bacteria harbouring p

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Simonova, Elena Vasil'evna, and Evgenija Nikolaevna Maksimova. "Microbiological monitoring of anthropogenically transformed soils." Samara Journal of Science 5, no. 1 (2016): 62–66. http://dx.doi.org/10.17816/snv20161113.

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The article presents results of microbiological monitoring of artificial soils. These soils were formed in the process of reclaiming technical lignin in special drives of the Baikal Pulp and Paper Mill (Irkutsk region). The test drives are in Solzanskom landfill, which is located between the vil. Solzan and the r. Bol'shaja Osinovka south of Irkutsk - Ulan-Ude highway. Investigations were carried out in drives № 1, № 4, № 5, № 6. Soil samples were homogenized in a selected point on three levels. Evaluation of sludge lignin was conducted in accordance with generally accepted sanitary and microb

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García-Ruiz, R., J. Lucena, and F. X. Niell. "Do bacteria regenerate phosphorus while decomposing seston?" Marine and Freshwater Research 50, no. 5 (1999): 459. http://dx.doi.org/10.1071/mf98077.

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The C:P ratio of both seston in suspension and seston collected by sediment traps were systematically higher than the Redfield ratio of 106 : 1 (molar) and decreased significantly with depth in the mesotrophic, phosphorus-limited La Concepción reservoir. An aerobic decomposition experiment of seston at different concentrations by a mixed population of reservoir bacteria was performed in batch cultures to investigate the bacterial role in the changes of the C:P ratio. Dissolved phosphorus was taken up during the bacterial growth phase, while carbon was transformed to CO2 . Therefore, the C:P ra

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Saleh, Saleema S., and Bernard R. Glick. "Involvement ofgacSandrpoSin enhancement of the plant growth-promoting capabilities ofEnterobacter cloacaeCAL2 and UW4." Canadian Journal of Microbiology 47, no. 8 (2001): 698–705. http://dx.doi.org/10.1139/w01-072.

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The plant growth-promoting bacteria Enterobacter cloacae CAL2 and UW4 were genetically transformed with a multicopy plasmid containing an rpoS or gacS gene from Pseudomonas fluorescens. The transformed strains were compared with the nontransformed strains for growth, indoleacetic acid (IAA) production, antibiotic production, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, siderophore production, cell morphology, and the ability to promote canola root elongation. All transformed strains had a longer lag phase, were slower in reaching stationary phase, and attained a higher cell

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Al –Mallah, Mozahim K., and Semaa Abd Al- Kader. "Electro-conjugation between Klebsiella pneumoniae and Proteus mirabilis and between Agrobacterium rhizogenes R1601 and Sinorhizobium meliloti." Journal of Biotechnology Research Center 6, no. 1 (2012): 80–87. http://dx.doi.org/10.24126/jobrc.2012.6.1.206.

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Bacterial conjugations represent one of the possibilities to produce transformed bacteria. This study aimed to detect the occurrence of conjugation between the pathogenic bacteria Klebsiella pneumoniae and Proteus mirabilis and between Agrobacterium rhizogenes R1601 and Sinorhizobium meliloti. The results proved the conjugation in those bacterial parents through the sensitivity of bacterial species to specific types of antibiotics. The obtained data reported the stimulation of their growth when exposed to different electrical pulses. These electrotreatments include three voltages (200, 250, 30

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Kay, Elisabeth, Timothy M. Vogel, Frank Bertolla, Renaud Nalin, and Pascal Simonet. "In Situ Transfer of Antibiotic Resistance Genes from Transgenic (Transplastomic) Tobacco Plants to Bacteria." Applied and Environmental Microbiology 68, no. 7 (2002): 3345–51. http://dx.doi.org/10.1128/aem.68.7.3345-3351.2002.

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ABSTRACT Interkingdom gene transfer is limited by a combination of physical, biological, and genetic barriers. The results of greenhouse experiments involving transplastomic plants (genetically engineered chloroplast genomes) cocolonized by pathogenic and opportunistic soil bacteria demonstrated that these barriers could be eliminated. The Acinetobacter sp. strain BD413, which is outfitted with homologous sequences to chloroplastic genes, coinfected a transplastomic tobacco plant with Ralstonia solanacearum and was transformed by the plant's transgene (aadA) containing resistance to spectinomy

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Nunes, Cátia, Luísa Gonçalves, and Paula Baptista. "Promising Bacteria for Glyphosate Degradation." Biology and Life Sciences Forum 4, no. 1 (2020): 82. http://dx.doi.org/10.3390/iecps2020-08872.

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Soil contamination is particularly serious. One of the main factors contributing to this is the accelerated development of agriculture, which has led to a strong increase in the use of herbicides. Studies show that herbicides formulated on the basis of glyphosate have a carcinogenic potential in laboratory animals, being classified by the WHO as a probable carcinogen for humans. In this context, our research works in the area of decontamination of agricultural soils, using native microorganisms, where the pollutants are transformed into by-products that are less harmful to the environment. A s

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Kazunga, Chikoma, and Michael D. Aitken. "Products from the Incomplete Metabolism of Pyrene by Polycyclic Aromatic Hydrocarbon-Degrading Bacteria." Applied and Environmental Microbiology 66, no. 5 (2000): 1917–22. http://dx.doi.org/10.1128/aem.66.5.1917-1922.2000.

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ABSTRACT Pyrene is a regulated pollutant at sites contaminated with polycyclic aromatic hydrocarbons (PAH). It is mineralized by some bacteria but is also transformed to nonmineral products by a variety of other PAH-degrading bacteria. We examined the formation of such products by four bacterial strains and identified and further characterized the most apparently significant of these metabolites.Pseudomonas stutzeri strain P16 and Bacillus cereus strain P21 transformed pyrene primarily tocis-4,5-dihydro-4,5-dihydroxypyrene (PYRdHD), the first intermediate in the known pathway for aerobic bacte

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Dissertations / Theses on the topic "Bacteria transformed"

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Carranza, Masso Laura Carolina. "Standardization and internal validation of a bacteria identification method utilizing focal-plane-array fourier transformed infrared spectroscopy." Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=106248.

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Food-borne diseases collectively affect significant portions of the world's population. In Canada recent food-borne outbreaks and diseases resulted in significant expenses and resource allocations. Consequently, finding methods that can detect and identify microorganisms in faster, reliable and cost effective ways is a pressing need. Microbial identification methods based on the infrared spectral analysis of microorganisms have been shown to be potentially viable. The recent development of focal plane array Fourier transform infrared (FPA-FTIR) spectroscopy provided the means of acquiring thou

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Alliotte, Thierry. "Contribution a l'etude de l'expression genetique chez la plante : identification et caracterisation de quatre genes presentant un profil d'expression remarquable." Paris 7, 1988. http://www.theses.fr/1988PA077001.

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Garip, Sebnem. "The Characterization Of Bacteria With Fourier Transform Infrared(ftir) Spectroscopy." Master's thesis, METU, 2005. http://etd.lib.metu.edu.tr/upload/12606673/index.pdf.

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New and rapid techniques for the characterization and identification of bacteria would have an important role in clinical microbiology and in food analysis because of an increasing prevalence of infectious diseases and In this work we carried out two approaches. In the first study the characterization and differentiation of mesophilic and thermophilic bacteria were investigated by using Fourier Transform Infrared (FTIR) Spectroscopic technique. In the second study, we investigated the characterization and identification of 3 Bacillus and Micrococcus species Our results from first approach show

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De, Castro Therese C. "Analysis of Arabidopsis plants transformed with AtPRX34 for defence against bacterial wilt." Diss., University of Pretoria, 2011. http://hdl.handle.net/2263/27515.

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Pinchuk, Tommy. "Optimization of pre-processing variables for hyperspectral analysis of focal plane array Fourier transform infrared images." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=98769.

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A genetic algorithm was employed to select the optimal combination of preprocessing variables, including data pretreatment, data manipulation and feature extraction procedures, for eventual clustering of a data set consisting of hyperspectral images acquired by a focal plane array Fourier transform infrared (FPA-FTIR) spectrometer. The data set consisted of infrared images of bacterial films, and the classification task investigated was the discrimination between Gram-positive and Gram-negative bacteria. The genetic algorithm evaluated combinations of variables pertaining to bacterial film thi

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Sang, Shu-Chih. "The in-situ infrared microspectroscopy of bacterial colonies on agar plates." Virtual Press, 1996. http://liblink.bsu.edu/uhtbin/catkey/1014855.

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The purpose of this research was to develop a more convenient method to distinguish bacteria using Fourier transform infrared (FT-IR) spectroscopy. The specular reflectance infrared spectra of bacterial colonies were obtained in-situ, without removing them from the agar growth media. The spectra of a variety of bacterial species were obtained by the infrared microscope and then were analyzed by factor analysis. Using this statistical method in conjunction with in-situ sampling we evaluated how well Grampositive species were sorted from Gram-negative species. Also, how the type of agar used and

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Mitra, Adinpunya. "4-hydroxycinnamoyl-CoA hydratase/lyase from Pseudomonas fluorescens AN103 : characterisation and effects of expression in transformed root cultures of Datura stramonium." Thesis, University of East Anglia, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301994.

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Carlsson, Sofia. "Surface Characterization ofGram-Negative Bacteria andtheir Vesicles : Using Fourier Transform Infrared Spectroscopy andDynamic Light Scattering." Thesis, Umeå universitet, Kemiska institutionen, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-64292.

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Pegram, Sarah. "Use of Fourier Transform Infrared Spectroscopy for the Classification and Identification of Bacteria of Importance to the Food Industry." DigitalCommons@USU, 2007. http://digitalcommons.usu.edu/etd/73.

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The aim of this work was to use Fourier Transform Infrared Spectroscopy to characterize and identify bacteria of particular significance to the food industry. FT-IR spectroscopy is a rapid technique that can be applied to all groups of bacteria. The two objectives were to determine a suitable sampling procedure to record a spectrum and to determine a suitable statistical technique to identify characteristic regions of the spectrum associated with the genus and, potentially, the species. Pure cultures of bacteria were grown in broth, suspended in saline and dried to produce a film on a halide s

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Prévost, Kirkwood Jonah. "Identification of bacteria by infrared imaging with the use of focal plane array Fourier transform infrared spectroscopy." Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=111855.

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The application of infrared imaging employing focal plane array Fourier transform infrared (FPA-FTIR) instrumentation for the identification of bacteria was investigated. FPA-FTIR spectroscopy was shown to provide new opportunities for bacteria identification with unprecedented reliability and throughput by allowing 102--103 FTIR spectra to be acquired simultaneously from surface areas of 90 x 90 to 200 x 200 mum with a spatial resolution of &sim;6 mum. The combination of data redundancy and spatial resolution afforded by infrared imaging made it possible to acquire highly reproducible spectra

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Books on the topic "Bacteria transformed"

1

John, Waller. The discovery of the germ: Twenty years that transformed the way we think about disease. Icon, 2002.

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The first miracle drugs: How the sulfa drugs transformed medicine. Oxford University Press, 2006.

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Lesch, John E. The first miracle drugs: How the sulfa drugs transformed medicine. Oxford University Press, 2005.

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The Discovery of the Germ: Twenty Years That Transformed The Way We Think About Disease (Revolutions in Science). Columbia University Press, 2003.

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Waller, John. The Discovery of the Germ: Twenty Years That Transformed the Way We Think About Disease (Revolutions in Science). Columbia University Press, 2005.

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Archibald, John. Genomics: A Very Short Introduction. Oxford University Press, 2018. http://dx.doi.org/10.1093/actrade/9780198786207.001.0001.

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Genomics has transformed the biological sciences. From epidemiology and medicine to evolution and forensics, the ability to determine an organism’s complete genetic makeup has changed the way science is done and the questions that can be asked of it. Genomics: A Very Short Introduction explores the science of genomics and its rapidly expanding toolbox. Sequencing a human genome can now take only a few days and those of simple bacteria and viruses, a matter of hours. The resulting sequences can be used to better understand our biology in health and disease and to ‘personalize’ medicine. This VS

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Transforma tu salud : la clave está en las bacterias intestinales y las hormonas. Grijalbo, 2018.

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Transforma tu salud : la clave está en las bacterias intestinales y las hormonas. Grijalbo, 2018.

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Book chapters on the topic "Bacteria transformed"

1

Ardeleanu, M., R. Morisset, and L. Bertrand. "Fourier Transform Infrared Photoacoustic Spectra of Bacteria." In Photoacoustic and Photothermal Phenomena III. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-540-47269-8_22.

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Schmid, Katherine M. "Dihydrosterculate in Tobacco Transformed with Bacterial Cyclopropane Fatty Acid Synthase." In Plant Lipid Metabolism. Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-015-8394-7_31.

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Goff, Steve. "Assay of Bacterial Chloramphenicol Acetyl Transferase in Transformed Maize Tissues." In The Maize Handbook. Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4612-2694-9_110.

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Amiel, C., J. Travert, M. C. Curk-Daubie, P. Pichon, D. Lefier, and L. Mariey. "Identification of Lactic Acid Bacteria by Fourier Transform Infrared Spectroscopy (FTIR)." In Spectroscopy of Biological Molecules: New Directions. Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4479-7_251.

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Amiel, C., M. C. Curk, C. Denis, J. C. Ingouf, J. Reyrolle, and J. Travert. "Identification and Classification of Bacteria by Fourier Transform Infrared Spectroscopy (FTIR)." In Spectroscopy of Biological Molecules: Modern Trends. Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5622-6_194.

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Mattioli, T. A., B. Robert, and M. Lutz. "Asymmetric Structural Aspects of the Primary Donor in Several Photosynthetic Bacteria: the Near-IR Fourier Transform Raman Approach." In The Photosynthetic Bacterial Reaction Center II. Springer US, 1992. http://dx.doi.org/10.1007/978-1-4615-3050-3_15.

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Picand, C., J. Lebaud, G. Novel, and J. Travert. "Differentiation, Identification and Classification of Bacteria by Fourier Transform Infrared Spectroscopy (FT-IR)." In Spectroscopy of Biological Molecules. Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0371-8_234.

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Geethanjali, M., V. Kannan, and A. V. R. Anjana. "Bacterial Foraging Optimization Algorithm Trained ANN Based Differential Protection Scheme for Power Transformers." In Swarm, Evolutionary, and Memetic Computing. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-27242-4_31.

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Tamas, I. A., W. H. R. Langridge, S. D. Abel, et al. "Hormonal control of apical dominance. Studies in tobacco transformed with bacterial luciferase and Agrobacterium rol genes." In Progress in Plant Growth Regulation. Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2458-4_49.

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Bourne, David J., Eliane Abou-Mansour, Russell T. Hill, and Peter T. Murphy. "Dereplication and Profiling of Marine Bacteria by Fatty Acid Analysis of Crude Extracts Using Fourier Transform Mass Spectrometry." In New Developments in Marine Biotechnology. Springer US, 1998. http://dx.doi.org/10.1007/978-1-4757-5983-9_11.

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Conference papers on the topic "Bacteria transformed"

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Krebsbach, Meaghen A., Karim H. Muci-Küchler, and Brandon J. Hinz. "Influence of Projectile Momentum and Kinetic Energy on Bacterial Distribution in an Extremity Surrogate “Wound Track”." In ASME 2012 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/imece2012-89142.

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This paper presents an experimental study that examines the relationship between the initial momentum and the initial kinetic energy of a projectile and the distribution of bacterial contamination along a “wound track” created in an extremity surrogate representative of the superior (upper) portion of the lower leg (i.e., the calf region) of an average adult human male. Initial surface contamination was represented using circular filter paper moistened with a solution containing 5 × 106 colony forming units per milliliter (CFU/ml) of Escherichia coli strain K-12 that was previously transformed

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Abir, Mirazul Mahmud, Yuichi Otsuka, and Yukio Miyashita. "Effects of Composition on Antibacterial and Antiviral Properties of Suspension Plasma-Sprayed Hydroxyapatite/Titania Coating." In ITSC2021, edited by F. Azarmi, X. Chen, J. Cizek, et al. ASM International, 2021. http://dx.doi.org/10.31399/asm.cp.itsc2021p0585.

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Abstract This study investigates the effect of composition on the antibacterial and antiviral properties of hydroxyapatite/titania composite coatings deposited by suspension plasma spraying. Hydroxyapatite is a bioceramic material used as a plasma-sprayed coating to promote osseointegration of femoral stems. TiO2 has promising photocatalytic activity and good efficiency in destroying bacteria, viral species, and parasites. Prior to coating, substrates were grit blasted, ultrasonically cleaned, and heated to enhance adhesion strength. The microstructure of the resulting coatings was then charac

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Gamburg, K. Z., V. A. Bybin, G. B. Borovskii, and Yu A. Markova. "EFFECTS OF POTATO (SKARB VARIETY) TRANSFORMED WITH GOX GENE FROM PENICILLIUM FISTULOSUM ON THE GROWTH OF NONPATHOGENIC ESHERICHIA COLI AND PHYTOPATHOGENIC PECTOBACTERIUM CAROTOVORUM AND CLAVIBACTER MICHIGANENSIS BACTERIA." In The All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-319-8-893-897.

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Kamnev, A. A., and A. V. Tugarova. "Intracellular transformations in bacteria as a response to external factors: molecular spectroscopic characterization." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.110.

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Baranskaya, M. I., L. A. Chaikovskaya, and N. N. Klimenko. "Primary evaluation of a new strain of phosphate-mobilizing bacteria." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-104.

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An initial assessment of new strains of phosphate-mobilizing bacteria isolated from the rhizosphere of cereals was carried out in the laboratory. According to the results of the quantitative evaluation of the phosphate solubilizing activity of bacteria, we found that 12 strains have the greatest ability to transform Ca3(PO4)2 into a soluble form. The highest Кr value was noted for strain 0613: the coefficient of phosphate solubilizing activity – 10. The maximum content of soluble (212 mg/l P2O5) phosphorus was in the culture liquid of strain 1702.

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Fabian, Heinz, Anne-Marie Gilles, Octavian Barzu, and Henry H. Mantsch. "Fine differences in structure and stability of bacterial adenylate kinases as revealed by IR difference spectroscopy." In Fourier Transform Spectroscopy: Ninth International Conference, edited by John E. Bertie and Hal Wieser. SPIE, 1994. http://dx.doi.org/10.1117/12.166609.

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Buzalewicz, Igor, Katarzyna Wysocka, and Halina Podbielska. "Exploiting of optical transforms for bacteria evaluation in vitro." In European Conference on Biomedical Optics. OSA, 2009. http://dx.doi.org/10.1364/ecbo.2009.7371_1h.

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Buzalewicz, Igor, Katarzyna Wysocka, and Halina Podbielska. "Exploiting of optical transforms for bacteria evaluation in vitro." In European Conferences on Biomedical Optics, edited by Christian D. Depeursinge and I. Alex Vitkin. SPIE, 2009. http://dx.doi.org/10.1117/12.831777.

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Gwak, Ho-Jin, and Mina Rho. "Transformer-based embedding applied to classify bacterial species using sequencing reads." In 2022 IEEE International Conference on Big Data and Smart Computing (BigComp). IEEE, 2022. http://dx.doi.org/10.1109/bigcomp54360.2022.00084.

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Samuels, Alan C., A. Peter Snyder, Diane St. Amant, et al. "Classification of select category A and B bacteria by Fourier transform infrared spectroscopy." In SPIE Defense and Security Symposium, edited by Augustus Way Fountain III and Patrick J. Gardner. SPIE, 2008. http://dx.doi.org/10.1117/12.767292.

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Reports on the topic "Bacteria transformed"

1

Shapira, Roni, Judith Grizzle, Nachman Paster, Mark Pines, and Chamindrani Mendis-Handagama. Novel Approach to Mycotoxin Detoxification in Farm Animals Using Probiotics Added to Feed Stuffs. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7592115.bard.

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T-2 toxin, a toxic product belongs to the trichothecene mycotoxins, attracts major interest because of its severe detrimental effects on the health of human and farm animals. The occurrence of trichothecenes contamination is global and they are very resistant to physical or chemical detoxification techniques. Trichothecenes are absorbed in the small intestine into the blood stream. The hypothesis of this project was to develop a protecting system using probiotic bacteria that will express trichothecene 3-O-acetyltransferase (Tri101) that convert T-2 to a less toxic intermediate to reduce inges

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Zhou, Ting, Roni Shapira, Peter Pauls, Nachman Paster, and Mark Pines. Biological Detoxification of the Mycotoxin Deoxynivalenol (DON) to Improve Safety of Animal Feed and Food. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7613885.bard.

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The trichothecene deoxynivalenol (DON, vomitoxin), one of the most common mycotoxin contaminants of grains, is produced by members of the Fusarium genus. DON poses a health risk to consumers and impairs livestock performance because it causes feed refusal, nausea, vomiting, diarrhea, hemolytic effects and cellular injury. The occurrence of trichothecenes contamination is global and they are very resistant to physical or chemical detoxification techniques. Trichothecenes are absorbed in the small intestine into the blood stream. The overall objective of this project was to develop a protecting

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Anderson, Olin, and Gad Galili. Development of Assay Systems for Bioengineering Proteins that Affect Dough Quality and Wheat Utilization. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568781.bard.

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The quality and utilization of wheat is largely dependent upon the exact physical/chemical properties of the doughs made from flour/water mixtures. Among the wheat seed components most correlated with dough visoelastic parameters are the high-molecular-weight (HMW) glutenin subunits whose disulfide cross-linked macropolymer is critical for dough functionality. We have used the tools of molecular biology, wheat transformation, heterologous expression of HMW-glutenin subunits in bacteria, and dough micro-mixing experiments to examine some of the molecular basis of HMW-glutenin functionality. In

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Reisch, Bruce, Avichai Perl, Julie Kikkert, Ruth Ben-Arie, and Rachel Gollop. Use of Anti-Fungal Gene Synergisms for Improved Foliar and Fruit Disease Tolerance in Transgenic Grapes. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7575292.bard.

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Original objectives . 1. Test anti-fungal gene products for activity against Uncinula necator, Aspergillus niger, Rhizopus stolonifer and Botrytis cinerea. 2. For Agrobacterium transformation, design appropriate vectors with gene combinations. 3. Use biolistic bombardment and Agrobacterium for transformation of important cultivars. 4. Characterize gene expression in transformants, as well as level of powdery mildew and Botrytis resistance in foliage of transformed plants. Background The production of new grape cultivars by conventional breeding is a complex and time-consuming process. Transfer

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Manulis-Sasson, Shulamit, Christine D. Smart, Isaac Barash, Laura Chalupowicz, Guido Sessa, and Thomas J. Burr. Clavibacter michiganensis subsp. michiganensis-tomato interactions: expression and function of virulence factors, plant defense responses and pathogen movement. United States Department of Agriculture, 2015. http://dx.doi.org/10.32747/2015.7594405.bard.

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Clavibactermichiganensissubsp. michiganensis(Cmm), the causal agent of bacterial wilt and canker of tomato, is the most destructive bacterial disease of tomato causing substantial economic losses in Israel, the U.S.A. and worldwide. The goal of the project was to unravel the molecular strategies that allow Cmm, a Gram-positive bacterium, to develop a successful infection in tomato. The genome of Cmm contains numerous genes encoding for extracellular serine proteases and cell wall degrading enzymes. The first objective was to elucidate the role of secreted serine proteases in Cmm virulence. Mut

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Adam, Zach, and Eran Pichersky. Degradation of Abnormal Proteins in Chloroplasts of Higher Plants. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568768.bard.

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In this study we attempted to get a better understanding of processes involved in the degradation of abnormal proteins i chloroplasts. To achieve this goal, we used a number of complementary approaches. We first characterized the expression of the two subunits of Clp protease. We demonstrated that both of them were expressed in chloroplasts in a constitutive fashion, but the expression of the regulatory subunit ClpC was enhanced by light. We generated a mutant the lumenal protein OEE33 which was targeted to the stroma in in vitro experiments. In the wrong compartment it was found unstable, and

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Jander, Georg, Gad Galili, and Yair Shachar-Hill. Genetic, Genomic and Biochemical Analysis of Arabidopsis Threonine Aldolase and Associated Molecular and Metabolic Networks. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7696546.bard.

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Since the amino acids threonine and isoleucine can be limiting in mammalian diet and there is interest in increasing their abundance in certain crop plants. To meet this need, a BARD proposal was written with two main research objectives: (i) investigate new avenues for manipulating threonine and isoleucine content in plants and (ii) study the role of threonine aldolase in plant metabolism. Research conducted to meet these goals included analysis of the sub-cellular localization of threonine aldolase in the plant, analysis of metabolic flux in developing embryos, over- and under-expression of

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