Academic literature on the topic 'Bacterial Artificial Chromosome (BAC)'

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Journal articles on the topic "Bacterial Artificial Chromosome (BAC)"

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Song, Junqi, Fenggao Dong, and Jiming Jiang. "Construction of a bacterial artificial chromosome (BAC) library for potato molecular cytogenetics research." Genome 43, no. 1 (2000): 199–204. http://dx.doi.org/10.1139/g99-099.

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Lack of reliable techniques for chromosome identification is the major obstacle for cytogenetics research in plant species with large numbers of small chromosomes. To promote molecular cytogenetics research of potato (Solanum tuberosum, 2n = 4x = 48) we developed a bacterial artificial chromosome (BAC) library of a diploid potato species S. bulbocastanum. The library consists of 23 808 clones with an average insert size of 155 kb, and represents approximately 3.7 equivalents to the potato genome. The majority of the clones in the BAC library generated distinct signals on specific potato chromo
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Narayanan, Kumaran, and Qingwen Chen. "Bacterial Artificial Chromosome Mutagenesis Using Recombineering." Journal of Biomedicine and Biotechnology 2011 (2011): 1–10. http://dx.doi.org/10.1155/2011/971296.

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Gene expression from bacterial artificial chromosome (BAC) clones has been demonstrated to facilitate physiologically relevant levels compared to viral and nonviral cDNA vectors. BACs are large enough to transfer intact genes in their native chromosomal setting together with flanking regulatory elements to provide all the signals for correct spatiotemporal gene expression. Until recently, the use of BACs for functional studies has been limited because their large size has inherently presented a major obstacle for introducing modifications using conventional genetic engineering strategies. The
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Hanson, Robert E., Michael S. Zwick, Sangdun Choi, et al. "Fluorescent in situ hybridization of a bacterial artificial chromosome." Genome 38, no. 4 (1995): 646–51. http://dx.doi.org/10.1139/g95-082.

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Fluorescent in situ hybridization (FISH) of a 130 kilobase cotton (Gossypium hirsutum L.) bacterial artificial chromosome (BAC) clone containing a high proportion of single-copy DNA produced a large pair of FISH signals on the distal end of the long arm of a pair of chromosomes of the D-genome species G. raimondii Ulbr. and produced a fainter pair of signals on a small submetacentric pair of chromosomes of the A-genome species G. herbaceum L. The signals were syntenic with a nucleolar organizer region in G. raimondii and G. herbaceum. Signal pairs were easily recognized in interphase and metap
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Murrell, Isa, Gavin S. Wilkie, Andrew J. Davison, et al. "Genetic Stability of Bacterial Artificial Chromosome-Derived Human Cytomegalovirus during CultureIn Vitro." Journal of Virology 90, no. 8 (2016): 3929–43. http://dx.doi.org/10.1128/jvi.02858-15.

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ABSTRACTClinical human cytomegalovirus (HCMV) strains invariably mutate when propagatedin vitro. Mutations in gene RL13 are selected in all cell types, whereas in fibroblasts mutants in the UL128 locus (UL128L; genes UL128, UL130, and UL131A) are also selected. In addition, sporadic mutations are selected elsewhere in the genome in all cell types. We sought to investigate conditions under which HCMV can be propagated without incurring genetic defects. Bacterial artificial chromosomes (BACs) provide a stable, genetically defined source of viral genome. Viruses were generated from BACs containin
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Gschwend, Andrea R., Qingyi Yu, Paul Moore, et al. "Construction of Papaya Male and Female BAC Libraries and Application in Physical Mapping of the Sex Chromosomes." Journal of Biomedicine and Biotechnology 2011 (2011): 1–7. http://dx.doi.org/10.1155/2011/929472.

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Papaya is a major fruit crop in the tropics and has recently evolved sex chromosomes. Towards sequencing the papaya sex chromosomes, two bacterial artificial chromosome (BAC) libraries were constructed from papaya male and female genomic DNA. The female BAC library was constructed using restriction enzymeBstY I and consists of 36,864 clones with an average insert size of 104 kb, providing 10.3x genome equivalents. The male BAC library was constructed using restriction enzymeEcoR I and consists of 55,296 clones with an average insert size of 101 kb, providing 15.0x genome equivalents. The male
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Janes, Daniel E., Nicole Valenzuela, Tariq Ezaz, Chris Amemiya, and Scott V. Edwards. "Sex Chromosome Evolution in Amniotes: Applications for Bacterial Artificial Chromosome Libraries." Journal of Biomedicine and Biotechnology 2011 (2011): 1–6. http://dx.doi.org/10.1155/2011/132975.

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Variability among sex chromosome pairs in amniotes denotes a dynamic history. Since amniotes diverged from a common ancestor, their sex chromosome pairs and, more broadly, sex-determining mechanisms have changed reversibly and frequently. These changes have been studied and characterized through the use of many tools and experimental approaches but perhaps most effectively through applications for bacterial artificial chromosome (BAC) libraries. Individual BAC clones carry 100–200 kb of sequence from one individual of a target species that can be isolated by screening, mapped onto karyotypes,
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Qian, Yaping, Li Jin, and Bing Su. "Construction and characterization of bacterial artificial chromosome library of black-handed spider monkey (Ateles geoffroyi)." Genome 47, no. 2 (2004): 239–45. http://dx.doi.org/10.1139/g03-122.

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The large-insert genomic DNA library is a critical resource for genome-wide genetic dissection of target species. We constructed a high-redundancy bacterial artificial chromosome (BAC) library of a New World monkey species, the black-handed spider monkey (Ateles geoffroyi). A total of 193 152 BAC clones were generated in this library. The average insert size of the BAC clones was estimated to be 184.6 kb with the small inserts (50-100 kb) accounting for less than 3% and the non-recombinant clones only 1.2%. Assuming a similar genome size with humans, the spider monkey BAC library has about 11×
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Meksem, Khalid, Jeffry Shultz, Faiza Tebbji, et al. "A bacterial artificial chromosome based physical map of the Ustilago maydis genome." Genome 48, no. 2 (2005): 207–16. http://dx.doi.org/10.1139/g04-099.

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Ustilago maydis, a basidiomycete, is a model organism among phytopathogenic fungi. A physical map of U. maydis strain 521 was developed from bacterial artificial chromosome (BAC) clones. BAC fingerprints used polyacrylamide gel electrophoresis to separate restriction fragments. Fragments were labeled at the HindIII site and codigested with HaeIII to reduce fragments to 50–750 bp. Contiguous overlapping sets of clones (contigs) were assembled at nine stringencies (from P ≤ 1 x 10–6 to 1 x 10–24). Each assembly nucleated contigs with different percentages of bands overlapping between clones (fro
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Zhang, Peng, Wanlong Li, Bernd Friebe, and Bikram S. Gill. "Simultaneous painting of three genomes in hexaploid wheat by BAC-FISH." Genome 47, no. 5 (2004): 979–87. http://dx.doi.org/10.1139/g04-042.

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Fluorescence in situ hybridization (FISH) is widely used in the physical mapping of genes and chromosome landmarks in plants and animals. Bacterial artificial chromosomes (BACs) contain large inserts, making them amenable for FISH mapping. In our BAC-FISH experiments, we selected 56 restriction fragment length polymorphism (RFLP)-locus-specific BAC clones from the libraries of Triticum monococcum and Aegilops tauschii, which are the A- and D-genome donors of wheat (Triticum aestivum, 2n = 6x = 42), respectively. The BAC clone 676D4 from the T. monococcum library contains a dispersed repeat tha
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Gómez, Martha I., M. Nurul Islam-Faridi, Sung-Sick Woo, et al. "FISH of a maize sh2-selected sorghum BAC to chromosomes of Sorghum bicolor." Genome 40, no. 4 (1997): 475–78. http://dx.doi.org/10.1139/g97-063.

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Fluorescence in situ hybridization (FISH) of a 205 kb Sorghum bicolor bacterial artificial chromosome (BAC) containing a sequence complementary to maize sh2 cDNA produced a large pair of FISH signals at one end of a midsize metacentric chromosome of S. bicolor. Three pairs of signals were observed in metaphase spreads of chromosomes of a sorghum plant containing an extra copy of one arm of the sorghum chromosome arbitrarily designated with the letter D. Therefore, the sequence cloned in this BAC must reside in the arm of chromosome D represented by this monotelosome. This demonstrates a novel
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Dissertations / Theses on the topic "Bacterial Artificial Chromosome (BAC)"

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Trapp, Sascha. "Klonierung und Mutagenese des Bovinen Herpesvirus Typ 1 als ein infektiöses künstliches bakterielles Chromosom (bacterial artificial chromosome, BAC)." Diss., lmu, 2003. http://nbn-resolving.de/urn:nbn:de:bvb:19-11529.

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McDowell, Erin. "Characterization of a bacterial artificial chromosome (BAC)-based infectious clone of a low passage Marek's disease virus (MDV) vaccine strain, CVI988." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 106 p, 2009. http://proquest.umi.com/pqdweb?did=1885544371&sid=6&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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Taylor, Suzanne. "Towards the construction of a bacterial artificial chromosome (BAC) library of Fragaria vesca L. to isolate a gene controlling seasonal flowering." Thesis, University of Reading, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369543.

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Guo, Yufang. "Quantitative genetic analysis for flowering time in primitive Upland cotton, Gossypium hirsutum L., and chromosome assignment of BAC-derived SSR markers." Diss., Mississippi State : Mississippi State University, 2007. http://library.msstate.edu/etd/show.asp?etd=etd-11062007-142438.

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Somridhivej, Benjaporn Liu Zhanjiang. "Characterization, polymorphism assessment, and database construction for microsatellites from BAC end sequences of catfish a resource for integration of linkage and physical maps /." Auburn, Ala., 2007. http://repo.lib.auburn.edu/Send%2011-10-07/SOMRIDHIVEJ_BENJAPORN_30.pdf.

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Penha, Helen Alves. "Construção de uma biblioteca genômica de Passiflora edulis f. flavicarpa inserida em BACs (Bacterial Artificial Chromosome) e mapeamento cromossômico usando hibridação in situ fluorescente\"." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-25092012-101518/.

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Passiflora (Passifloraceae) é um grande gênero de espécies vegetais encontradas, principalmente, na flora tropical. Algumas passifloras são cultivadas como plantas ornamentais, frutíferas ou exploradas pelas suas propriedades medicinais. A principal espécie comercial brasileira, o maracujá-azedo (Passiflora edulis f. flavicarpa, 2n = 18), ocupa 95% da área plantada. Os frutos são consumidos in natura ou processados pela indústria de suco. Estudos genéticos e cromossômicos têm sido gerados para esta espécie. Entretanto, devido ao pequeno tamanho e a similaridade morfológica dos seus cromossomos
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Bittorf, Blaine E. "Mapping Hybrid Lethal Genes on the X Chromosome of C. Briggsae." Wright State University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=wright152770556182685.

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Dougherty, John Kelly. "Identification of a Hybrid Lethal Gene on the X Chromosome of Caenorhabditis briggsae." Wright State University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=wright1579011194671611.

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Mammadov, Jafar. "Towards Cloning the Leaf Rust Resistance Gene Rph5." Diss., Virginia Tech, 2004. http://hdl.handle.net/10919/28704.

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Leaf rust caused by Puccinia hordei is an important disease of barley (Hordeum vulgare) in many regions of the world. Yield losses up to 62% have been reported in susceptible cultivars. The Rph5 gene confers resistance to the most prevalent races (8 and 30) of barley leaf rust in the United States. Therefore, the molecular mapping of Rph5 is of great interest. Genetic studies were performed by analysis of 93 and 91 F2 plants derived from the crosses 'Bowman' (rph5) x 'Magnif 102' (Rph5) and 'Moore' (rph5) x Virginia 92-42-46 (Rph5), respectively. Linkage analysis positioned the Rph5 locus to t
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Tonga, Lavon Paongo Hutt-Fletcher Lindsey M. "Development of an Akata-based bacterial artificial chromosome." Diss., UMK access, 2006.

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Thesis (Ph. D.)--School of Biological Sciences. University of Missouri--Kansas City, 2006.<br>"A dissertation in molecular biology and biochemistry and cell biology and biophysics." Advisor: Lindsey M. Hutt-Fletcher. Typescript. Vita. Title from "catalog record" of the print edition Description based on contents viewed Nov. 9, 2007. Includes bibliographical references (leaves 89-103). Online version of the print edition.
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Books on the topic "Bacterial Artificial Chromosome (BAC)"

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Shaying, Zhao, and Stodolsky Marvin, eds. Bacterial artificial chromosomes. Humana Press, 2004.

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Bacterial artificial chromosomes. Humana Press, 2004.

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(Editor), Shaying Zhao, and Marvin Stodolsky (Editor), eds. Bacterial Artificial Chromosomes: Volume 2: Functional Studies (Methods in Molecular Biology). Humana Press, 2004.

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Bacterial Artificial Chromosomes (Methods in Molecular Biology (Clifton, N.J.), V. 255-256.). Humana Pr, 2004.

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Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Sequencing (Methods in Molecular Biology). Humana Press, 2004.

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Book chapters on the topic "Bacterial Artificial Chromosome (BAC)"

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Sung, Myong-Hee. "Bacterial Artificial Chromosome (BAC)." In Encyclopedia of Systems Biology. Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_757.

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Choi, Sangdun, and Rod A. Wing. "The Construction of Bacterial Artificial Chromosome (BAC) Libraries." In Plant Molecular Biology Manual. Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4217-5_1.

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Beil, Jane, and Thorsten Buch. "Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1215-5_8.

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Ruzsics, Zsolt, Frederic Lemnitzer, and Christian Thirion. "Engineering Adenovirus Genome by Bacterial Artificial Chromosome (BAC) Technology." In Adenovirus. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-679-5_11.

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Biradar, Siddanagouda S., Xiaojun Nie, Kewei Feng, and Song Weining. "Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-715-0_6.

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Ritland, Kermit, Nima Farzaneh, Claire Cullis, et al. "Back to BACs: Conifer Genome Exploration with Bacterial Artificial Chromosomes." In Compendium of Plant Genomes. Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-21001-4_7.

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Yang, Zhongan, Hong Jiang, and Shuo Lin. "Bacterial Artificial Chromosome Transgenesis for Zebrafish." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-977-2_7.

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Cottingham, Matthew G. "Genetic Manipulation of Poxviruses Using Bacterial Artificial Chromosome Recombineering." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-876-4_3.

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Accardo, Maria Carmela, and Patrizio Dimitri. "Fluorescence in situ Hybridization with Bacterial Artificial Chromosomes (BACs) to Mitotic Heterochromatin of Drosophila." In Methods in Molecular Biology. Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-789-1_30.

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Stankiewicz, Elzbieta, Tianyu Guo, Xueying Mao, and Yong-Jie Lu. "Fluorescence In Situ Hybridization and Rehybridization Using Bacterial Artificial Chromosome Probes." In Methods in Molecular Biology. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9769-5_16.

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Conference papers on the topic "Bacterial Artificial Chromosome (BAC)"

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Balya, David, and Botond Roska. "High throughput design of Bacterial Artificial Chromosomes (BACs) for cell type specific gene expression in transgenic mice." In 2006 IEEE Biomedical Circuits and Systems Conference - Healthcare Technology (BioCas). IEEE, 2006. http://dx.doi.org/10.1109/biocas.2006.4600313.

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