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1

Yıldırım, Musa, and Hacer Bilir Özhan. "EFFECT OF BACTERIAL CURING AND BACTERIAL ADDITIVE ON CONCRETE PROPERTIES." Advances in Civil and Architectural Engineering 14, no. 27 (2023): 32–43. http://dx.doi.org/10.13167/2023.27.3.

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In this study, calcium carbonate was formed on the surfaces and inner structure of concrete using the microbially induced carbonate precipitation method. Bacillus megaterium bacteria were supplemented into the curing water and concrete mixtures. Three types of concrete were tested: control concrete, bacteria-containing concrete, and concrete cured in bacterial liquid. Compressive strength, water absorption, capillary water absorption, scanning electron microscopy (SEM), and mapping analyses were conducted to investigate the effects of bacterial additive or bacterial curing to concrete specimen
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2

Kabir, Aliyu, Adamu Lawan, Jibrin Kaura, and Ibrahim Aliyu. "Effect of Calcite Precipitation on Properties of Fresh and Hardened Laterized Concrete." Nigerian Journal of Engineering 30, no. 3 (2023): 16. http://dx.doi.org/10.5455/nje.2023.30.03.03.

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Reduction in strength and durability is the limitation for use on laterized concrete in various engineering applications.This research focuses on the effect of calcite precipitation on the strength and durability of laterized concrete. The laterized concrete sample was prepared by fine aggregate replacement with laterite at 0 %, 10 %, 20 %, 30 %, and 40 % by weight of fine aggregate. A prescribed concrete mix proportion of 1:1.5:3 and a water-to-cement ratio of 0.5 was used to prepare the concrete. At each level of replacement, Sporosarcina Pasteurii bacteria at concentrations of 0, 1.5 x 108,
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3

Das, Prayash, Indrajeet Das, Mithilesh Kumar, Jagannath Pradhan, and Chitrabhanu Sahoo. "Bacterial Concrete." International Journal for Research in Applied Science and Engineering Technology 10, no. 5 (2022): 1120–23. http://dx.doi.org/10.22214/ijraset.2022.42323.

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Abstract: Cement mortar durability is the function of its internal pore structure and distribution, porosity and its permeation properties. Research has shown that some specific bacterial species isolatedfrom soil can tolerate harsh and challenging alkaline environment and can be used in remediating cracks in cement mortar structures. This state of-the-art microbial based crack healing mechanism is one such phenomenon on which studies were carried out to investigate the role of calcite mineral precipitation in improvement of durability in bacteria integrated cement mortar. Keywords: Bacteria,
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Sarma, Swapneel, Baharul Hussain, SharminaAktara Begum, et al. "Comparative Study of Mechanical Properties of Concrete Using Commercially Available Bacterial Strains." Proceedings of the 12th Structural Engineering Convention, SEC 2022: Themes 1-2 1, no. 1 (2022): 1311–15. http://dx.doi.org/10.38208/acp.v1.656.

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Bacteria impregnated concrete is a recent advancement in concrete technology which is being studied extensively by different researchers all around the world. This study aims to study the effect of bacteria on different parameters of concrete and compare the results from compressive test, split tensile test and flexure test with that of a normal mix concrete. The main objective of this research was to study the mechanical properties of concrete upon adding commercially available Bacillus Subtilis (5 billion cells/gm) and laboratory cultured Bacillus Cohnii (105 cells/ml) to the concrete mix. B
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G. Ghoneim, Amr, Hilal A. Hassan, and Louay A. Aboul-Nour. "Self-repairing polyethylene fiber-reinforced-concrete with bacillus subtilis bacteria a review." International Journal of Engineering & Technology 9, no. 2 (2020): 437. http://dx.doi.org/10.14419/ijet.v9i2.30172.

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Fibers and bacterial additives in concrete have achieved significant success as a construction material. This paper presents the field of concrete self-repairing by introducing both Bacillus subtilis bacteria and polyethylene fiber as a dual-components. The main research goal is to reveal the principles of concrete self-repairing. At first, the research investigates the fiber-reinforced-concrete behavior, the concrete self-repairing process with the Bacillus subtilis bacteria for forming bacterial-concrete. And then, the study highlights the damage-repairing numerical simulation of fiber-reinf
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6

Bhanusuresh, H. S. "Study on bacteria based self-healing properties of bio-concrete - An overview." i-manager’s Journal on Civil Engineering 13, no. 1 (2023): 25. http://dx.doi.org/10.26634/jce.13.1.19319.

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The use of bacteria-based self-healing concrete has gained attention in recent years due to its potential to improve the durability and sustainability of concrete structures. This paper provides an overview of the research conducted on the self-healing properties of bacteria-based bio-concrete. The paper discusses the mechanism of bacterial self-healing in concrete, the types of bacteria used in self-healing concrete, and the methods used to introduce bacteria into the concrete. The paper also reviews comparative studies that evaluate the mechanical properties and durability of selfhealing bac
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7

Balasubramaniam N, Boobalan SC, Prabakaran E, and Rajeshkumar V. "Mechanical and Microstructural Analysis of Self-Compacting Concrete." International Research Journal of Multidisciplinary Scope 06, no. 01 (2025): 1486–97. https://doi.org/10.47857/irjms.2025.v06i01.02121.

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Concrete possesses pores and the propensity to develop microcracks, both of which are very undesirable since they facilitate the penetration through water along with additional harmful elements into the building element. Using Microbiologically Induced Calcite Precipitation (MICP), the Bacterial Self Compacting technique is a potential way to fill concrete fractures. To promote the deposition of calcium carbonate within the concrete material, microorganisms use the urease enzyme in this process. Bacterial remediation is a long-term, ecologically safe, and bio-based treatment that works better
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8

Qtiashat, Deya, Mahmoud Al-Khazaleh, P. Krishna Kumar, Ali Alqatawna, and Islam A. Alshafei. "Influence of Bacillus Subtilis Bacteria on Strength and Durability of Concrete with Silica Fume." Civil Engineering Journal 11, no. 5 (2025): 1959–68. https://doi.org/10.28991/cej-2025-011-05-013.

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This study investigates the influence of Bacillus subtilis bacteria on the strength and durability properties of M30 concrete with and without silica fume. The experimental study was conducted on four concrete mix series: conventional concrete (B1), conventional concrete with silica fume (B2), bacterial concrete without any admixtures (B3), and bacterial concrete with silica fume (B4). Silica fume was incorporated at replacement levels of 5% and 10% by weight of cement for the B2 and B4 mix series to evaluate its effect on bacterial activity and concrete performance. The study measured compres
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9

Talluri, Rajesh, Prathap Mathangi, and Venkateshwarlu Musini. "A Critical Review on Bacterial Concrete." International Journal of Scientific Engineering and Research 5, no. 8 (2017): 64–69. https://doi.org/10.70729/8071701.

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10

Rahaman, Sk, Datunaka Sai Srujan, Jayati Ray Dutta, Arkamitra Kar, and Mohna Bandyopadhyay. "Cell Viability Studies on Bacillus sp. under Different Storage Conditions for Usage in Improving Concrete Compressive Strength." Buildings 13, no. 9 (2023): 2392. http://dx.doi.org/10.3390/buildings13092392.

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Bacterial concrete is a possible approach toward sustainability in concrete construction through crack-healing. Including a bacterial culture as an admixture in concrete can enhance the service life of a structure through the self-healing of cracks. Incorporating bacterial cells as an admixture in concrete is a major challenge as bacteria are living organisms with a limited shelf-life. It is essential to evaluate the shelf-life of bacterial cultures to encourage the inclusion of bacteria in concrete applications. Hence, the main focus of this study was to record the cell viability of these mic
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11

Sharma, Vaidik, and Prof Mayur Singi. "Experimental Study on Bacterial based Self-Healing Concrete." International Journal for Research in Applied Science and Engineering Technology 11, no. 7 (2023): 40–46. http://dx.doi.org/10.22214/ijraset.2023.54587.

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Abstract: The potential of concrete to self-heal has been studied in several works, hence in this study we are analyzing the strength properties of self-healing bacterial concrete by mixing bacteria (Bacillus Subtilis) with calcium source. In the study, bacteria (Bacillus Subtilis) were introduced at 3% and 5%, while calcium lactate was added at 5% and 10%, respectively. As a result, the study addresses economic issues not just for building but also for upkeep. The purpose of this experiment was to look into the strength qualities of bacterial self-healing concrete with varied ratios of Bacill
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Vempada, Srinivasa Reddy, Meduri V. Seshagiri Rao, Shrihari Saduwale, Pothula Mahesh, T. V. Suneetha, and Darya Viktorovna Nemova. "Pore structure characterization of bacterial concrete." MATEC Web of Conferences 392 (2024): 01002. http://dx.doi.org/10.1051/matecconf/202439201002.

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This work describes an experimental investigation on the impact of calcifying bacteria on permeation properties (such as absorptivity and sorptivity) of bacterial concrete. In order to evaluate the improvement of permeation properties owing to biomineralization in bacterial concrete, tests for sorptivity, porosity, water absorption capacity, and ordinary (M20), standard (M40), and high strength (M60 and M80) grade bacteria were then conducted on the concrete samples treated with the bacteria. The findings demonstrate the bacterial concrete's porosity, sorptivity, and absorptivity are significa
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Shukla, Ashish, Nakul Gupta, Saurav Dixit, et al. "Effects of Various Pseudomonas Bacteria Concentrations on the Strength and Durability Characteristics of Concrete." Buildings 12, no. 7 (2022): 993. http://dx.doi.org/10.3390/buildings12070993.

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The goal of this study is to improve concrete’s efficiency by using a microbiologically produced specific growth/filler. One such way of thinking has resulted in the invention of a highly unusual concrete known as microbial concrete, which uses bacteria to cure flaws in the concrete. Investigators working with various microorganisms suggested several microbial concretes. The bacterium “Pseudomonas” was used in this experiment. Pseudomonas bacteria, which can make calcite precipitates on a proper medium supplied with a calcium supply, is a typical science lab bacterium for calcite generation. T
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14

Kumar, J. Suresh, S. Ramesh, and K. Shobana. "An empirical study on the mechanical and durability characteristics of M50 grade high-strength bacterial concrete using Bacillus bacteria." Materials Express 14, no. 8 (2024): 1188–97. https://doi.org/10.1166/mex.2024.2729.

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Concrete is commonly utilised in construction. Curing strengthens and shrinks concrete. Reactive liquids enter concrete through shrinkage cracks. These fluids lower core pH, corroding reinforcing bars under ideal conditions. Therefore, concrete crack repair and structural integrity restoration methods must be developed. Currently, the use of synthetic polymers to repair environmentally detrimental cracks has prompted the advancement of biological therapy methods. These synthetic polymers affect the environment. The study seeks to improve concrete quality and mend cracks sustainably. In this st
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15

Srijan, Shrivastava, and Harsh Rathore Dr. "Exploring the Role of Bacteria in Concrete Repair and Durability: A Review of Bio-Concrete Innovations." Journal of Advances in Geotechnical Engineering 8, no. 1 (2024): 1–7. https://doi.org/10.5281/zenodo.14273308.

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<em>Bacterial concrete, an innovative approach in construction materials, utilizes microbial-induced calcite precipitation (MICP) to enhance the durability and strength of concrete. This process involves incorporating urease-producing bacteria, such as Bacillus pasteurii or Sporosarcina pasteurii, into concrete, which induces the formation of calcium carbonate (CaCO3) crystals that fill cracks and voids, thereby improving the material&rsquo;s structural integrity. This review explores the mechanisms underlying MICP, the influence of various bacterial species, and their effects on the physical
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16

Xu, Hongyin, Jijian Lian, Maomao Gao, Dengfeng Fu, and Yue Yan. "Self-Healing Concrete Using Rubber Particles to Immobilize Bacterial Spores." Materials 12, no. 14 (2019): 2313. http://dx.doi.org/10.3390/ma12142313.

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Bacteria-based self-healing concrete is a construction material used to repair cracks in concrete, in which the bacterial spores are immobilized by bacteria carriers. However, the currently available bacteria carriers are not always suitable due to a complicated procedure or high cost. To develop a more suitable bacteria carrier as well as improve the anti-crack capability of self-healing concrete, in this study we evaluate the feasibility of using rubber particles as a novel bacteria carrier in self-healing concrete. Two types of self-healing concrete are prepared with rubber particles of dif
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17

Prajakta, Chandrashekhar Wani, Nivrutti Wani Chandrashekhar, and Chandrashekhar Wani Prajwal. "Emerging Trends in Civil Engineering." International Journal of Innovative Science and Research Technology 7, no. 4 (2022): 421–23. https://doi.org/10.5281/zenodo.6508854.

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In this century India has developed more as I mean is technology has developed in India. There is the development of emerging trends in civil construction that is Bacterial Concrete by emerging crushed aggregate. As we know concrete is one of the most common material used in every construction and the main issue with the concrete is that it suffers from the cracks. Therefore, this paper reviews the types of Bacteria used in concrete for healing of the cracks. This paper also gives the idea about the properties of concrete after addition of different types of Bacterias and also replacing coarse
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18

Putra, Andri Prima, Teuku Budi Aulia, Yunita Idris, and Erfan Handoko. "Effect of Using Types and Concentrations of Ureolytic Bacteria on Flexural Capacity of High-Strength Concrete with Self-Healing Concrete Capabilities." IOP Conference Series: Earth and Environmental Science 1444, no. 1 (2025): 012017. https://doi.org/10.1088/1755-1315/1444/1/012017.

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Abstract Concrete’s deflection and cracking are influenced by flexural capacity. High-strength concrete tends to have high hydration rate, making it susceptible to microcracks, thus, early maintenance is required. The weak nature of concrete against tensile forces allows microcracks to occur and can propagate into macrocracks if the cracks are not detected. Therefore, an innovation emerged called self-healing concrete enabling concrete to cover microcracks independently. This method can be used to heal concrete cracks, involving bacteria in concrete mixture that are active when cracks occur by
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19

Prafulla, Padvi, and Jeswani Hansa. "Bacillus Induced Calcium Carbonate Precipitate for Healing of Concrete." IOP Conference Series: Earth and Environmental Science 1280, no. 1 (2023): 012025. http://dx.doi.org/10.1088/1755-1315/1280/1/012025.

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Abstract Concrete is good in compression but weak in tension. The strength of concrete can be enhanced by using admixtures and biological end products during the process of mixing. The cracks appear in concrete even after taking proper measures. Bacterial induction of calcium carbonate precipitate can be a solution to cure cracking as well as increase the strength of concrete. Microbial induction using specific species of bacteria can enhance the strength of concrete as well as give it the ability to self heal the cracks by calcium carbonate precipitate. In this research paper, bacterial cells
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Chen, How-Ji, Ching-Fang Peng, Chao-Wei Tang, and Yi-Tien Chen. "Self-Healing Concrete by Biological Substrate." Materials 12, no. 24 (2019): 4099. http://dx.doi.org/10.3390/ma12244099.

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At present, the commonly used repair materials for concrete cracks mainly include epoxy systems and acrylic resins, which are all environmentally unfriendly materials, and the difference in drying shrinkage and thermal expansion often causes delamination or cracking between the original concrete matrix and the repair material. This study aimed to explore the feasibility of using microbial techniques to repair concrete cracks. The bacteria used were environmentally friendly Bacillus pasteurii. In particular, the use of lightweight aggregates as bacterial carriers in concrete can increase the ch
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Espitia Nery, Martín Eduardo, Dery Esmeralda Corredor Pulido, Paula Andrea Castaño Oliveros, Johan Andrey Rodriguez Medina, Querly Yubiana Ordoñez Bello, and Maikol Santiago Perez Fuentes. "Mechanisms of encapsulation of bacteria in self-healing concrete: review." DYNA 86, no. 210 (2019): 17–22. http://dx.doi.org/10.15446/dyna.v86n210.75343.

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Fissures in concrete structures result from structural deterioration and inadequate building processes, among other factors. Traditional in situ repair is often expensive and complex. For this reason, self-healing techniques have been developed, such as the use of bacteria that precipitate calcium carbonate and seal fissures. However, adding bacteria directly to the concrete matrix reduces bacterial survival. We present a review of different methods of bacterial encapsulation and their effects on fissure repair and concrete resistance. We argue that encapsulation of Bacillus subtilis in clay i
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Adil, Md Raghib, Supia Khatun, Mohsin Jamal, and Abhijit Mondal. "Investigation on Self-Healing Concrete Using Bacillus Subtilis Bacteria." MATEC Web of Conferences 400 (2024): 01002. http://dx.doi.org/10.1051/matecconf/202440001002.

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Cracking is a common occurrence in concrete, primarily attributed to its relatively low tensile strength due to External loads which induce high tensile stresses. Immediate and proper treatment is essential to prevent the expansion of cracks, as their unchecked growth may lead to higher repair costs. Bacterially induced calcium carbonate precipitation emerges as an eco-friendly alternative for crack repair. This technique involves the metabolic activities of specific bacteria in concrete, resulting in microbial mineral precipitation. In this study an attempt has been made to define bacterial c
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Bharathi, Kodavati Divya, Dhondy Rupesh Kumar, Polapali Saikiran, and Belli Karthik. "Mechanical Properties of Bacterial Concrete by Partial Replacement of Cement by Nano Silica in Different Grades of Concrete." Ecology, Environment and Conservation 29 (2023): 504–10. http://dx.doi.org/10.53550/eec.2023.v29i01s.077.

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Concrete with nano-silica also has a higher strength compared with normal concrete. In the current study, an examination of the mechanical characteristics of bacterial concrete containing nano-silica is conducted. The impact of concrete was examined in this study employing Bacillus subtilis for self-Healing Microbial Type Culture Collection (MTCC) Strain no.121, Among the bacteria concentrations of 104 , 105 , and 106 cells/ ml, a concentration of 105 cells/ml gives more strength, so further investigation is done using 105 cells/ml cell concentration for different percentages of nano-silica is
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24

Shobana, K., and R. Thenmozhi. "Experimental investigation of self-repairing bio concrete in self compacting concrete." Materials Express 12, no. 5 (2022): 705–12. http://dx.doi.org/10.1166/mex.2022.2197.

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Concrete is a fundamental component of public infrastructure and a necessary building material. When exposed to tension, traditional concrete has a defect that causes it to crack. Concrete can withstand compressive pressures well, but not tensile forces. Concrete cracking is a serious problem in the building industry. The best method to conserve energy and safeguard the environment in terms of sustainable development is to make concrete structures more durable and easy to maintain. According to a recent study, by precipitating the chemical calcium carbonate through a process known as bio miner
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Jakubovskis, Ronaldas, Augusta Jankutė, Simona Guobužaitė, Renata Boris, and Jaunius Urbonavičius. "Prolonging Bacterial Viability in Biological Concrete: Coated Expanded Clay Particles." Materials 14, no. 11 (2021): 2719. http://dx.doi.org/10.3390/ma14112719.

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One of the biggest challenges in the development of a biological self-healing concrete is to ensure the long-term viability of bacteria that are embedded in the concrete. In the present study, a coated expanded clay (EC) is investigated for its potential use as a bacterial carrier in biological concrete. Eight different materials for coatings were selected considering cost, workability and accessibility in the construction industry. Long-term (56 days) viability analysis was conducted with a final evaluation of each coating performance. Our results indicate that healing efficiency in biologica
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Rowell, Austen, Tewodros Ghebrab, and Randall Jeter. "Bacterial Treatment of Recycled Concrete Aggregate." Recycling 8, no. 5 (2023): 68. http://dx.doi.org/10.3390/recycling8050068.

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Microbial treatment of recycled concrete aggregate (RCA) may improve the quality of the aggregate, and enhance its use in the production of structural concrete and promote the recycling of concrete waste. The mortar phase of the RCA is responsible for the poor performance of the aggregate. Treating the old adhered mortar or removing it from the natural aggregate (NA) is an option to make RCA beneficial for the production of quality recycled aggregate concrete (RAC). Removing the adhered mortar from recycled concrete aggregate using silicate-solubilizing bacteria was investigated. The bacteria
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27

Raut, Atharv. "Self-Healing Concrete." International Journal for Research in Applied Science and Engineering Technology 13, no. 4 (2025): 3501–8. https://doi.org/10.22214/ijraset.2025.69038.

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Crack formation is very common phenomenon in concrete structure which allows the water and different type of chemical into the concrete through the cracks and decreases their durability, strength and which also affect the reinforcement when it comes in contact with water, CO2 and other chemicals. Cracks in concrete structures can significantly decrease their lifespan by exposing reinforcement to outside environment, leading to concrete degradation. To address this issue, self-healing techniques have been developed, including Biomineralization-based-self healing, where bacteria are employed to
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Tiwari, Sachin, Shilpa Pal, Rekha Puria, Vikrant Nain, and Rajendra Prasad Pathak. "Mechanical and Microstructure Study of the Self Healing Bacterial Concrete." Materials Science Forum 969 (August 2019): 472–77. http://dx.doi.org/10.4028/www.scientific.net/msf.969.472.

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Concrete largely used for construction material, degrades with the development of cracks that becomes easy passage for entry of chemicals and harmful compounds. Self healing capability is helpful to mitigate the deterioration of the concrete structures. This research work focuses on the self healing behaviour and mechanical properties of the bioconcrete supplemented with three different bacteria namely Bacillus sphaericus, Bacillus cohnii and Bacillus megaterium. Concrete supplemented with Bacillus cohnii exhibited 35.31% increase in compressive strength compared to control mix after 28 days.
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29

Prajeesha M.P. "Evaluation of Bacterial Concrete Corrosion Resistance in Marine Settings." Harbin Gongcheng Daxue Xuebao/Journal of Harbin Engineering University 45, no. 07 (2024): 145–57. https://doi.org/10.52783/jheu.v45i07.3229.

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Around the world, concrete is widely used as an essential building material, however it can fracture and let water and salts in, which can cause corrosion and shorten the structure's lifespan. In particular, the robustness of bacterial concrete in marine environments where it is exposed to more extreme conditions is examined in this extensive study. Making use of the special properties of bacteria that may produce calcium carbonate, bacterial concrete, also known as Bio-Concrete, takes advantage of this. Sealing the cracks helps the concrete self-heal, hence increasing the structure's lifespan
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30

Hritik, Deore. "Efficacy of Bacterial Concrete with Conventional Concrete." International Journal for Research in Applied Science and Engineering Technology 10, no. 7 (2022): 4846–53. http://dx.doi.org/10.22214/ijraset.2022.46081.

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Abstract: Concrete is one of the maximum used building substances. But, it is one of the predominant manufacturers of carbon dioxide (CO2) that's immediately contributing to destroying our surroundings. Not to say that significant costs are being spent each year to keep concrete structures. Cracks of numerous sizes shape in all concrete structures which need to be sealed manually shortening the life of a particular construction. However, Self-healing concrete (SHC) is a innovative constructing material that has the answer to these kind of troubles and is the constructing fabric of the near des
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31

Zhang, Weng, Ding, and Qian. "Use of Genetically Modified Bacteria to Repair Cracks in Concrete." Materials 12, no. 23 (2019): 3912. http://dx.doi.org/10.3390/ma12233912.

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In this paper, we studied the crack-repair by spraying bacteria-based liquid around the cracks in concrete. To enhance the repair efficiency and speed up the repair process, the transposon mutagenesis method was employed to modify the genes of Bacillus halodurans and create a mutant bacterial strain with higher efficiency of calcium carbonate productivity by catalyzing the combination of carbonate and calcium ion. The efficiency of crack-repairing in concrete by spraying two kinds of bacterial liquid was evaluated via image analysis, X-ray computed tomography (X-CT) scanning technology and the
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32

Musurmonov, Abror Alisherovich, Jahongir Olimjon o'g'li Choriyev, and Shohista Hidoyatillo qizi Ubaydullayeva. "FUTURE BUILDING MATERIAL: BIO CONCRETE SELF-HEALTHING." JOURNAL OF UNIVERSAL SCIENCE RESEARCH 1, no. 4 (2023): 6–15. https://doi.org/10.5281/zenodo.7791773.

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Crack occurrence in reinforced concrete should be minimized for both durability and economical reasons as crack repair is costly. Autogenous repair, or self-healing, of concrete would save a substantial amount of money, as manual inspection and crack repair could be minimized. Thus, a reliable self-healing mechanism for concrete would not only result in more durable structures, but would also be beneficial for the global economy. This study exploited the potential to apply calcite-precipitating bacteria as a crack-healing agent in concrete. The potential of different species to precipitate cal
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Kadian, Amarender, and Sandeep Pannu. "A Study of Durability Properties of Bacterial Concrete." Journal of Advances and Scholarly Researches in Allied Education 15, no. 3 (2018): 78–81. http://dx.doi.org/10.29070/15/56796.

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Ivaškė, Augusta, Ronaldas Jakubovskis, Renata Boris, and Jaunius Urbonavičius. "Effects of Low Temperature, Freeze–Thaw Cycles, and Healing Conditions on Viability of Non-Ureolytic Bacteria in Biological Self-Healing Concrete." Materials 17, no. 23 (2024): 5797. http://dx.doi.org/10.3390/ma17235797.

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The capacity of biological self-healing concrete (BSHC) to repair cracks relies on the sustained viability and metabolic function of bacteria embedded within the concrete. BSHC structures face significant risk in cold climates due to low temperatures and freeze–thaw (FT) cycles, during which freezing water can generate internal pressure that damages bacterial cells and diminishes their activity. A special feature of this study is the incorporation of bacterial spores within expanded clay aggregates, tested under varying environmental conditions. The viability of bacterial spores was measured u
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Jang, Indong, Dasom Son, Yongjun Son, Jihyeon Min, and Chongku Yi. "Use of Methylcellulose-Based Pellet to Enhance the Bacterial Self-Healing of Cement Composite." Materials 14, no. 20 (2021): 6113. http://dx.doi.org/10.3390/ma14206113.

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In this study, a new type of bacterial carrier using methylcellulose was presented, and its applicability to self-healing concrete has been explored. Methylcellulose, the main component of a 2 mm pellet-shaped carrier, can remain stable in alkaline environments and expand in neutral or acidic environments. These properties allow bacteria to survive in the high-alkaline and high-pressure environments of early age concrete, and the number of bacteria increases rapidly in the event of cracks, accelerating crack closure. The results show that the survival rate of bacterial spores inside the mortar
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Gopal, P. Hara, D. Mouli, K. Bhaskara Rao, K. Sunil Kumar, M. Raj, and P. P. S. Sowmya. "An Experimental Approach to Study the Properties of Self Healing Concrete by Replacing Fine Aggregate with Glass Powder and Demolished Waste." International Journal for Research in Applied Science and Engineering Technology 11, no. 4 (2023): 805–9. http://dx.doi.org/10.22214/ijraset.2023.50213.

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Abstract: Bacterial concrete is a material, which can successfully remediate cracks in concrete. This technique is highly desirable because the mineral precipitation induced as a result of microbial activities is pollution free and natural. To repair the cracks in concrete is a tedious job and in turn is expensive. So to avoid these, a special bacteria is induced in the concrete which reacts with calcium to form calcium carbonate crystals which blocks the cracks formed in the concrete. To make the Bacterial Concrete more affective in crack reduction, we used glass powder as partial replacement
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Et. al., Ms P. Kala. "Stress–Strain Behaviour of Bacterial Concrete Incorporated With Sugarcane Fibres." Turkish Journal of Computer and Mathematics Education (TURCOMAT) 12, no. 3 (2021): 5596–606. http://dx.doi.org/10.17762/turcomat.v12i3.2231.

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Bacterial concrete is one of the methods of rectifying the micro-cracks developed in the structural elements made of concrete. The gram-positive type bacteria Bacillus subtilis when acquainted with concrete produces calcite precipitation which heals the micro cracks in the concrete. Bacillus subtilis was used with a cell concentration of 106. The optimised percentage replacement of fine aggregates with sugarcane fibres of grain size less than 4.75 mm was 0.1 %. The effect of sugarcane fibres on the durability of bacterial concrete is presented in this paper.To study the Stress -Strain behaviou
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Jambhulkar, Pallavi Khemraj. "Literature Review on Self-Healing Concrete by Using Bacteria." International Journal for Research in Applied Science and Engineering Technology 13, no. 5 (2025): 1280–84. https://doi.org/10.22214/ijraset.2025.70006.

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The purpose of this literature paper is to determine the use of bacteria is one of the most rising as well as bright approaches. The primary component of a concrete construction is cement. In concrete structure crack development is a typical phenomenon, it reduces durability &amp; strength. Also analyse the impact of oxygen and water on the crack portion. In this study use of bacteria because the bacteria are having repair ability. This paper focuses on the mechanical properties of Bacillus family self-healing concrete. The mix proportion of material are taken while performing on bacterial bas
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Puram, Anchal. "An Experimental Investigation on Mechanical Properties of Bacterial Concrete Using Bacillus Subtilis." International Journal for Research in Applied Science and Engineering Technology 12, no. 5 (2024): 3296–301. http://dx.doi.org/10.22214/ijraset.2024.62338.

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Abstract: This Concrete is susceptible to micro crack formation and has pores which are highly undesirable because they provide an open pathway for the ingress of water and other deleterious substances. The use of concrete surface treatments with water proofing materials to prevent the access of aggressive substances is a common way of contributing to concrete durability. However, the most common surface treatments use organic polymers (epoxy, acrylics and polyurethanes) all of which have some degree of toxicity. Bio inspired materials can lead to a more sustainable construction industry, espe
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Linda, Tetty Marta, Syauqi Susana Rahmani, Andini Saras Wati, et al. "The Ureolytic Soil Bacteria Bacillus albus, a potential Agent for Biocement." HAYATI Journal of Biosciences 32, no. 3 (2025): 829–39. https://doi.org/10.4308/hjb.32.3.829-839.

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Concrete is a common building material and is very vulnerable to cracking caused by unstable temperature/humidity. Concrete crack repair can be done by using microorganism substitution that can produce CaCO3 (calcite) compounds that can be used as an environmentally friendly method in improving structural formation and increasing the strength and durability of concrete, one of which is using ureolytic bacteria. This study aimed to isolate and characterize ureolytic bacteria isolates and then to assess the calcite precipitation potential of ureolytic bacteria isolates from landfills. The ureoly
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Topçu, İlker Bekir Topçu, Tayfun Uygunoğlu, and Emre Kıvanç Budak. "Self Healing of Cracks in Concrete with Bacteria." European Journal of Formal Sciences and Engineering 3, no. 2 (2020): 65. http://dx.doi.org/10.26417/206rzh38o.

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During the service life of concrete structures, internal and external effects and micro-cracks occur in the structure. These cracks cause leakage of harmful substances into the concrete, deterioration of the strength and durability properties of the concrete, structural damages and crashes, and the high cost of maintenance and repair of the concrete structure. It is known that water-dissolved CO2 reacts with Ca+2 ions in the concrete and can repair the concrete by forming CaCO3 (limestone) crystals with very little water solubility. However, for this type of self-repair to occur, there must be
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Richardson, Alan, Leon Amess, Simon Neville, and Christopher Walton. "CRACK HEALING UTILISING BACTERIAL SPORES IN CONCRETE." Journal of Green Building 12, no. 3 (2017): 103–14. http://dx.doi.org/10.3992/1943-4618.12.3.101.

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This self repair system is based upon harmless ground borne bacteria as the self-healing agent. The bacteria are activated after the concrete is cracked and the bacterial spores are exposed to moisture and air. The bacterial reproduction process creates a calcite by-product which fills the cracks in the concrete. By sealing the cracks in concrete, an effective barrier to air or liquid borne deleterious materials are formed and as a consequence of this, enhanced durability is achieved in the structure, resulting in lower life cycle costs. The concrete/mortar prisms were cracked and tested for w
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Loan, Le Quynh, Nguyen Luong Hieu Hoa, Tran Thi My Ngoc, Duong Thi Phung Cac, and Nguyen Hoang Dung. "Isolation of calcite precipitation bacteria to improve the strength of concrete." Tạp chí Khoa học 14, no. 9 (2019): 143. http://dx.doi.org/10.54607/hcmue.js.14.9.297(2017).

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Concretes are the second most consumed material on earth. However, it is susceptible to micro crack formation and has pores in it. The repairing cracks in concrete require high cost and labor and traditional repair system are chemical based, expensive and lead to environmental and health hazards. In this study, the calcite precipitation bacteria were investigated. Bacterial strains were isolated from cement samples and were tested for urease activities, potential to form endospore and calcite precipitation. The results showed that four candidates were isolated with high urease activities and c
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Taku, Kumator, and Adamu Lawan. "Sustainable Concreting: Optimization Modelling of the Strength Properties of Bio-Self Compacting Concrete Incorporating Sporosarcina Pasteurii, Calcined Clay and Limestone Powder." Journal of Engineering Science 15, no. 1 (2024): 11–19. http://dx.doi.org/10.3329/jes.v15i1.76001.

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Sustainable concreting is prerequisite for infrastructural development in developing countries so as to meet up with the sustainable development goal of adequate mass housing and other critical infrastructure. Thus, research is ever ongoing aimed at developing cheaper and more durable concrete via the incorporation of bio-based by-products in concrete to improve its properties, as well as optimizing the quantities of these secondary materials for maximum and optimal concrete production. One such revolutionary concrete that is yet to find full application in the developing world is self-compact
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Nasytha, Amathalia Laiyina, Teuku Budi Aulia, and Yunita Idris. "The Effect of Using Ureolytic Bacteria as Self-Healing Agent in Concrete on Workability, Density and Absorption." Journal of Physics: Conference Series 2916, no. 1 (2024): 012025. https://doi.org/10.1088/1742-6596/2916/1/012025.

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Abstract Concrete is one of the fastest growing construction materials in Indonesia. Concrete has a low tensile strength that causes concrete to crack easily. One way to overcome this is by using self-healing concrete. This concrete is able to repair small cracks. Ureolytic bacteria can be used as a medium in self-healing concrete. These bacteria undergo a CaCO3 precipitation process to produce calcite which later fills the cracks in the concrete. This research aims to determine the effect of the application of ureolytic bacteria on the workability, density, and absorption of high-strength con
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Rosario, Roberto D., Arvin De La Cruz, and Mark P. De Guzman. "A Review of Biomineralization as Solution for Roads and Infrastructures Concrete Sustainability." Civil Engineering Journal 10, no. 8 (2024): 2745–60. http://dx.doi.org/10.28991/cej-2024-010-08-020.

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Concrete cracks in roads and infrastructure are ubiquitous due to environmental factors, fatigue, and material degradation. Applying bacteria with self-healing capabilities in concrete matrices is proposed as a solution. These bacteria, activated by water and oxygen ingress, produce calcium carbonate through biomineralization. They are improving structural integrity while reducing the adverse effects of chemical and water infiltration. The quantity of Bacillus bacteria to be added to the concrete mixture is an integral part of the standardization of the self-healing mechanism. 105 - 108 cells/
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Subhashini, S., K. K.Yaswanth, and D. S.V.Prasad. "Study on Strength and Durability Characteristics of Hybrid Fibre Reinforced Self-Healing Concrete." International Journal of Engineering & Technology 7, no. 4.2 (2018): 21. http://dx.doi.org/10.14419/ijet.v7i4.2.19993.

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The main disadvantage of using concrete, which is accepted to be irreplaceable building material, is the formation of micro cracks. This is due to the fact that concrete is weak in tension. To arrest the microcracks developed in the concrete and to eliminate the drawbacks due to microcracks, the recent trend in the innovation of the concrete is the usage of self-healing concrete or bacterial concrete. It is based on the principle that; the bacteria present in the moisture of the concrete repairs or heals the cracks on the concrete. Another recent advancement in the field of concrete technology
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Reddy, V. Srinivasa, Polina VVSSSR Krishna, G. Sai Karthik, and S. Shrihari. "Assessment of Corrosion Inhibiting Efficiency of Microbes Induced Concrete." E3S Web of Conferences 184 (2020): 01114. http://dx.doi.org/10.1051/e3sconf/202018401114.

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The study present in this paper reveals the corrosion inhibiting efficiency of M25 grade concrete induced with Sporosarcina pasteurii bacteria. The accelerated corrosion induced crack method is applied on reinforced bacterial concrete which is the modified philosophy of constant voltage technique. In the current investigation, for different cover thicknesses considered, total time required for charge passed until full longitudinal crack occurs along the cover thickness due to corrosion of steel reinforcement in concrete specimens are measured using which Charge Deterioration Factors (ChDFs) ar
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Vettrivelou, Mugil, B. Vijaya, M. Aruljothi, and Gokulakrishnan Janarthanan. "An Experimental Investigation on the Bacterial Concrete as an Innovative Approach to Self Crack Healing System." Materials Science Forum 1120 (April 18, 2024): 155–64. http://dx.doi.org/10.4028/p-4wgyud.

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Concrete structure are subjected to cracks and it is one of the immanent frailties of concrete thus reduces the life of concrete structure thereby results in high replacement cost. The study was inspired by the technique to find a remedy for cracking using bacteria namely Bacillus subtilis and Bacillus cereus on filling the voids, and the compressive strength, split tensile strength and flexural strength of bacterial impregnated concrete are compared with conventional concrete. The evaluated results of strength revealed that the use of bacteria in combination showed better improvement and SEM,
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Shome, Archana, Bhagyashree Deshpande, and Prashant Mundeja. "Comparative Evaluation Of Acid Resistance In Chemical And Bacterial Concrete: Strength, Durability, And Resilience Under Aggressive Environments." International Journal of Environmental Sciences 11, no. 10s (2025): 31–38. https://doi.org/10.64252/cr7mt588.

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This study investigates the durability and acid resistance of bacterial and conventional concrete under prolonged exposure to acidic environments. Concrete specimens were subjected to immersion in 5% H₂SO₄ and 5% HCl solutions for a period of 105 days, during which weight loss, compressive strength loss, Acid Durability Factor (ADF), and Acid Attack Factor (AAF) were evaluated. Bacterial concrete demonstrated superior performance compared to conventional concrete, showing significantly reduced weight loss and compressive strength degradation in both acid solutions.For ordinary grade concrete i
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