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Journal articles on the topic 'Bacterial inhibition zone'
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1
Rahmaniar, Reina Puspita, Dyah Widhowati, and Nurul Hidayah. "Garlic As Antibacterial in Lactose Fermenter Enterobacteriaceae." JURNAL KAJIAN VETERINER 11, no. 1 (2023): 47–53. http://dx.doi.org/10.35508/jkv.v11i1.11002.
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This study attempts to investigate the antibacterial effectiveness of three varieties of garlic (single garlic, kating garlic, and black garlic) on the growth of Enterobacteriaceae bacteria (Escherichia coli, Enterobacter sp, Klebsiella sp. from chicken meat samples. Treatment Kating garlic, single garlic, and black garlic at concentrations of 20%, 40%, 60%, 80%, 90%, 100%. This study observed the inhibition zone on Muller Hinton Agar (MHA) media that had been planted with bacterial isolates. The inhibition test uses the disk diffusion method. The findings revealed that kating garlic and single garlic showed inhibition zones against Escherichia coli, Enterobacter sp., and Klebsiella sp. bacteria. Meanwhile, black garlic did not show an inhibition zone against E. coli bacteria, but showed an inhibition zone against Klebsiella sp., Enterobacter sp., and Kating garlic had the largest bacterial inhibition zone against E. coli, Klebsiella sp., Enterobacter sp., followed by single garlic.
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Kashyap, Nand Kumar, Jeetendra Deepak, Arvind Kumar Bhardwaj, Milan Hait, and Dilipkumar Pal. "In-vitro Antibacterial and Antifungal Activity of Curcuma Amada Roxb. against Human Pathogens." Trends in Sciences 19, no. 22 (2022): 4839. http://dx.doi.org/10.48048/tis.2022.4839.
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This investigation was conducted to evaluate the antimicrobial effectiveness of the agar disc diffusion method and to determine the inhibition zone of Curcuma amada (CA) rhizome extracts (petroleum ether, dichloromethane, and chloroform) against 12 bacterial species and 4 fungal species. The inhibition zones of the extracts were compared to those of Ciprofloxacin and Griseofulvin for antibacterial and antifungal activities, respectively. The findings revealed that the organisms under examination showed significant inhibition of bacterial as well as fungal development during the experiment and that the typical form of C. amada rhizome can be used to treat bacterial and fungal illnesses. So, this plant could be used to find natural compounds that are bioactive and could be used to make new medicines. HIGHLIGHTS Extracts of amada rhizome were found to protect humans from pathogens aureus bacteria had the highest inhibition zone (17 mm) in dichloromethane extract, whereas B. subtilis and B. pumilus bacteria had the lowest inhibition zone (7.5 mm) in C. amada rhizome petroleum ether extract notatum in petrolieum ether extract and A. niger in chloroform extract had the highest inhibition zones (13.5 mm), whereas P. funiculosum in chloroform extract of C. amada rhizome had the lowest inhibition zone (10 mm) GRAPHICAL ABSTRACT
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Kadhim, Shurooq Rayyis. "Detection of The Antibacterial Activity of Bacteriocin from Local Isolates of Pseudomonas fluorescens Against Gram Positive Bacteria." Al Mustansiriyah Journal of Pharmaceutical Sciences 14, no. 1 (2014): 74–78. http://dx.doi.org/10.32947/ajps.v14i1.129.
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The antibacterial activity of local isolates of Pseudomonas fluorescens (P1and P2) were tested against some pathogenic bacteria, Staphylococcus aureus, S. epidermidis, Streptococcus faecalis, Streptococcus faecium isolated from stool urine and wounds by modified agar block method at 370C for 24 hr. The isolates of P. fluorescens were positive as producer of bacteriocin with a wide inhibition range on growth of gram positive pathogenic bacteria. P1 inhibited the bacterial growth of S. aureus isolates with a range of inhibition zone (12-20) mm. while P2 inhibited the bacterial growth with a range of inhibition zone was (13-22) mm .The range of inhibition zones of S.epidermidis by P1 were (10-18) mm. while the range by P2 was (9-20) mm. The isolate P1 inhibited the growth of all the tested isolates of S. faecalis with a range of inhibition zone between (12-19) mm. The range of inhibition zones of S. faecalis by P2 were (10-18) mm. The bacterial growth of S. faecium isolates were inhibited by P1 with a range of (15-19) mm. and (16-19) mm. with P2. Agar block method was suitable and easy for screening of bacteriocin production from this bacterium.
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Yulianto, Agus. "Extraction of Active Compounds from Mangrove Snail Meat (Terebralia sulcata) as an Antibacterial Agent Against Escherichia coli." Aquatic Life Sciences 1, no. 1 (2024): 8–12. http://dx.doi.org/10.58920/aqlis0101229.
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Bacterial Resistance is defined as the inability of antibiotics to inhibit bacterial growth systemically, mainly caused by synthetic chemical antibiotics. Therefore, research on pharmacological alternatives to synthetic antibiotics with natural antibacterial agents is needed, one of which is the crude extract of mangrove snail (T. sulcata) tested against Escherichia coli (E. coli) bacteria. This study conducted phytochemical screening and antibacterial potential testing of T. sulcata meat extract. T. sulcata was extracted with a polar solution of Methanol pa 1:4. Antibacterial testing against E. coli was performed at different extract concentration levels (5,000 ppm - 20,000 ppm). The results showed antibacterial activity with the formation of inhibition zones in the E. coli bacterial suspension medium. ANOVA analysis indicated that the mangrove T. sulcate extract at various concentrations influenced the growth of E. coli bacteria. Differences in treatment demonstrated an increase in extract concentration, which was directly proportional to the diameter of the inhibition zone. Observations of changes in the inhibition zone indicated that T. sulcata extract exhibited bacteriostatic properties against E. coli, characterized by a reduction in the diameter of the inhibition zone at each treatment concentration level. The formation of inhibition zones is believed to be due to the activity of active compounds such as alkaloids, flavonoids, saponins, phenols, and terpenoids.
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Rahma, Fanni tasya, Pratami Adityaningsari, and Firman Arifandi. "Test of Inhibitory Power of Virgin Coconut Oil and Olive Oil From Various Brands Against Staphylococcus aureus Bacteria, and The Review According to Islamic Prespective." Junior Medical Journal 1, no. 3 (2023): 274–81. http://dx.doi.org/10.33476/jmj.v1i3.2954.
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Infectious disease is a major public health problem in developed and developing countries. One of the bacteria that can cause infection is Staphylococcus aureus. Along with an increase in bacterial resistance, one of which is caused by the inappropriate use of antibiotics, it reduces the effectiveness and performance of treatment methods. Alternative medicine can use pure coconut oil (virgin coconut oil) which contains lauric acid as an antibacterial and olive oil which contains oleuropein as an antibacterial. Experimental research using the Kirby Bauer diffusion disc method by measuring the diameter of the inhibition zone of Staphylococcus aureus bacteria. The materials tested were various types of pure coconut oil and olive oil on the market with 50%, 75% and 100% inhibition zones. In the inhibition test of virgin coconut oil and olive oil, the bacterial inhibition zone was only found at a concentration of 100%, while at a concentration of 50% and 75% there was no bacterial inhibition of the two tested materials. The conclusion is that there is an effect of virgin coconut oil and olive oil on the inhibition of Staphylococcus aureus bacteria at a concentration of 100%.
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Maryani, Maryani, Mohamad Rozik, Milad Madiyawati, and Yuli Yuli. "Inhibition Test of Uncaria cordata (Lour.) Merr Extract Against Pathogenic Bacteria of Freshwater Fish In Vitro." Journal of Fish Health 4, no. 4 (2024): 200–207. https://doi.org/10.29303/jfh.v4i4.5832.
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Uncaria is a genus of plants that have medicinal properties and chemical content. Uncaria cordata (Lour.) Merr is a herbal plant that is still rarely known for its efficacy in herbal medicinal ingredients. Uncaria cordata (Lour.) Merr it self contains metabolic compounds in the form of flavonoid, phenolic, steroid, saponin, terpenoid and alkonoid. This study aims to test the effectiveness of Uncaria cordata (Lour.) Merr as an antimicrobial on Aeromonas hydrophila, Pseudomonas sp. and Edwardsiella ect.) Merr as an antimicrobial on Aeromonas hydrophila, Pseudomonas sp. and Edwardsiella ictaluri bacteria and the type of bacteria that are more effective in using Uncaria cordata (Lour.) Merr in inhibiting the proliferation of disease-causing bacteria in cultured fish. The results showed that Uncaria cordata (Lour.) Merr, was able to inhibit the proliferation of bacteria Aeromonas hydrophila, Pseudomonas sp. and Edwardsiella ictaluri with an average diameter of the inhibition zone of Aeromonas hydrophila bacteria at a concentration of 100% forming an inhibition zone of 21.7 mm with a very strong inhibition zone category, in the bacteria Pseudomonas sp. at 100% concentration formed an inhibition zone of 21 mm with a very strong inhibition zone category, and in Edwardsiella ictaluri bacteria at 100% concentration formed an inhibition zone of 21.3 mm with a very strong inhibition zone category. The 100% concentration of Uncaria cordata (Lour.) Merr is the most effective concentration to inhibit bacterial proliferation.
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Purba, Member Reni, Steven Wijaya, and Ananda Kasuma. "Potential of Red Betel Leaf Extract (Piper crocatum) and Siwak (Salvadora persica) Against Staphylococcus Aureus Bacteria." Bioscientia Medicina : Journal of Biomedicine and Translational Research 6, no. 5 (2022): 1728–31. http://dx.doi.org/10.37275/bsm.v6i5.504.
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Background. One of the bacteria that cause oral cavity infection is Staphylococcus aureus. This study aims to explore the antibacterial potential of red betel extract and siwak extract against Staphylococcus aureus bacteria, in vitro.
 Methods. This research is an in vitro experimental study to assess the diameter of the inhibition zone of red betel leaf extract and siwak against Staphylococcus aureus. The extract was made from red betel leaf simplicia and siwak wood with a dose of 50% each. The bacterial suspension was made by taking one pure colony from a test tube. Data analysis was performed using SPSS version 25 software.
 Results. Red betel leaf extract 50% showed the largest diameter of the inhibition zone, namely 9.1 ± 0.68 mm. Meanwhile, the siwak extract showed a smaller inhibition zone diameter of 7.9 ± 0.68 mm. One-way ANOVA analysis showed that there was a significant difference between the diameters of the inhibition zones of each test group.
 Conclusion. Red betel leaf extracts are 50% greater in inhibiting the growth of Staphylococcus aureus than 50% siwak extract.
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Wagh, Harshada. "Anti-oxidant and Anti-bacterial activity of polyherbal formulation." International journal of therapeutic innovation 2, no. 3 (2024): 0159–62. http://dx.doi.org/10.55522/ijti.v2i3.0051.
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The present work aimed to evaluate anti-oxidant and anti-bacterial activity of polyherbal formulation (gel) prepared using Tulsi, Aloe vera, Neem. It has been used for skin benefits since ancient times. Formulations were tested anti-oxidant activity by DPPH radical scavenging assay and anti-bacterial activity by disk diffusion Susceptibility Testing method. The anti-oxidant activity is exhibited in percentage inhibition. F1 formulation showed maximum percentage inhibition with increasing concentration as compared to standard ascorbic acid. The anti-bacterial activity is exhibited in zone of inhibition. The F5 formulation showed highest zone of inhibition (26.5 mm) against E. coli and zone of inhibition (26.0 mm) against Staphylococcus aureus which is an indication that F5 formulation is more effective on gram positive as well as negative bacteria.
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Wihayyu, Afri, Zurai Resti, Eri Sulyanti, Darnetty Darnetty, and Ujang Khairul. "Antagonistic Test of Endophytic Bacteria Against Fusarium oxysporum f.sp. cepae Causes of Moler Disease on Shallots." CROPSAVER - Journal of Plant Protection 7, no. 1 (2024): 35–42. http://dx.doi.org/10.24198/cropsaver.v7i1.54351.
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Fusarium oxysporum f.sp. cepae (FOCe) is a pathogenic fungus causing moler disease in shallots. One method of controlling this disease is by using endophytic bacteria. Endophytic bacteria are bacteria that live in plant tissues without causing disease symptoms. This research aims to obtain the most effective endophytic bacteria in inhibiting the growth of the pathogenic fungus FOCe. The study employs a Completely Randomized Design (CRD) consisting of 8 treatments with 3 replications each. The treatments include Bacillus sp. HI, Bacillus sp. SJI, Bacillus cereus P14, Bacillus cereus Se07, Bacillus subtilis, Serratia marcescens JB1E3, Serratia marcescens ULG1E4, and control. Tests conducted include the antibiosis test of endophytic bacterial cells using the dual culture method and the secondary metabolite test using media poisoning method. The observed parameters include inhibition zone, effectiveness of secondary metabolites, fresh weight, and dry weight of the FOCe fungus. All endophytic bacteria were capable of inhibiting the growth of FOCe. The most effective endophytic bacteria in inhibiting the growth of FOCe were found to be Serratia marcescens ULG1E4 and Serratia marcescens JB1E3. In the antibiosis test of endophytic bacterial cells, Serratia marcescens ULG1E4 exhibited an inhibition zone of 64.44%, while Serratia marcescens JB1E3 showed an inhibition zone of 61.11%. In the secondary metabolite compound test, the effectiveness values for Serratia marcescens ULG1E4 and Serratia marcescens JB1E3 were 95.31% and 95.03%, respectively.
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Azis, Arief, and Sellah Harselina. "UJI AKTIVITAS SEDIAAN MASKER GEL PEEL – OFF EKSTRAK DAUN KELOR (Moringa oleifera L.) TERHADAP Staphylococcus epidermidis." Jurnal Kesehatan Yamasi Makassar 7, no. 2 (2023): 9–18. http://dx.doi.org/10.59060/jurkes.v7i2.282.
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Moringa leaf extract was made in a peel-off gel mask preparation with a concentration of 10% and 15%. This study used a laboratory experimental method which was carried out at the Microbiology Laboratory of the Makassar Pharmacy Academy. Then the antibacterial activity of the peel-off gel mask was tested with Moringa leaf extract (Moringa oleifera L.) using the well method and Agar Medium (NA) as a bacterial medium and then incubated for 1x24 hours at 37oC. Furthermore, observations were made by measuring the inhibition zone that occurred at each concentration of the preparation on Staphylococcus epidermidis bacteria. The results showed that the inhibition zone of 10% concentration was 6.64 mm in diameter and at 15% concentration showed a diameter of 8.68 mm with the results of moderate category of bacterial inhibition. The conclusion of this study is that the peel-off gel mask of Moringa leaf extract (Moringa oleifera L.) can inhibit the growth of Staphylococcus epidermidis bacteria at concentrations of 10% and 15% as evidenced by the formation of an inhibition zone around the test wells, the most effective in inhibiting the growth of bacteria is at a concentration of 15%.
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JULYASIH, KETUT SRIE MARHAENI, NI PUTU RISTIATI, and IDA BAGUS PUTU ARNYANA. "Potensi Alga Merah dan Alga Hijau untuk Menghambat Pertumbuhan Bakteri Eschericia coli." Agrotrop : Journal on Agriculture Science 10, no. 1 (2020): 11. http://dx.doi.org/10.24843/ajoas.2020.v10.i01.p02.
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Potential of Red Algae and Green Algae to Inhibit Escherichia coli Bacteria Growth. The use of synthetic chemicals as a control for the growth of E. coli bacteria that causes of diarrhea children can cause adverse effects on health, so it needs natural control ingredients that do not cause impacts on human health. One natural controller to inhibit bacterial growth is by utilizing natural sources, including seaweed. The purpose of this study was to examine the potential of seaweed as an anti-bacterial agent of E. coli. The research used seaweed extract of Eucheuma spinosum, Euchuema cottonii, Gracilaria spp., and Caulerpa spp. The test parameters observed were the inhibition zone (mm) of each seaweed extract treatment using a ruler and measured the inhibition zone distance. The results showed that E.cottonii seaweed extract had the highest inhibitory ability with an inhibition zone diameter of 11.1 mm, including the moderately sensitive category, then Caulerpa spp. with a inhibition zone diameter of 10.0 mm, Gracilaria spp has the ability to inhibit E.coli bacteria with a inhibition zone diameter of 6.9 mm, including the insensitive category, and the lowest in seaweed E. spinosum with a inhibition zone diameter of 4.2 mm. including the insensitive category.
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Munira, Sofia. "CRUDE EXTRACTS OF MAGNOLIA ALBA FLOWERS USING N-HEXANE AND METHANOL SOLVENTS AND THEIR ANTI-ACNE POTENTIAL." Journal of Medical pharmaceutical and allied sciences 10, no. 6 (2021): 3789–93. http://dx.doi.org/10.22270/jmpas.v10i6.1607.
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This study aimed to test the antibacterial activity of n-hexane and methanol extracts of white cempaka flowers (Magnolia alba) against Propionibacterium acnes bacteria. The extract was obtained by using the maceration method for 72 hours. Each extract was tested for its antibacterial properties using the disc diffusion method with the variant concentration of the extract used was 0.05%; 2%; 4%; 6%; and 8%. The results showed that the methanol extract had a higher inhibition zone than the n-hexane extract. In n-hexane extract, the lowest concentration of 0.05% showed an inhibition zone of 6.6 mm and the highest concentration of 8% had an inhibition zone of 9.6 mm. Whereas in methanol extract, the inhibition zone obtained was 7.3 mm at a low concentration of 0.05%, but at the highest concentration of 8% the inhibition zone obtained was 11.6 mm. The effect of extract concentration on the diameter of the inhibition zone formed is very significant (directly proportional), where the higher the concentration used, the larger the diameter of the inhibition zone produced. Antibacterial testing for both extracts showed a weak (< 10 mm) to moderate (> 10 mm) bacterial inhibition, which was similar with the inhibition zone obtained from the positive control (11-11.3 mm; moderate bacterial inhibition).
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Mohammed, Ali Hussein, Nasser Salh Lhwak, and Ahmed Abdulhadi Jabbar. "Extraction and identification of certain bioactive compounds with antibacterial activity from the green algae Cladophora glomerate." Medicra (Journal of Medical Laboratory Science/Technology) 7, no. 1 (2024): 19–23. http://dx.doi.org/10.21070/medicra.v7i1.1742.
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Cladophora glomerata algae extract was used to inhibit the growth of five bacterial species: Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Citrobacter, and Klebsiella pneumoniae. Algae samples were collected from the river Euphrates in Al-Fadhiliya/Thi-Qar governorate and extracted into alcohol. The concentrations of the extracts were compared with five antibiotics. The 200% concentration showed the highest effectiveness against bacteria, with an inhibition zone of 28mm. The antibiotics Imipenem and Meropenem had an inhibition zone of 28mm, while Amikacin, Norfloxacin, and Piperacillin had an inhibition area of 12-21mm. The 20% concentration was least effective against E. coli, with an inhibition diameter of 2mm. The alcoholic extract of C. glomerata alga had the highest inhibition region of 28 against K. pneumonia and 13mm against P. mirabilis. The chemical algal contents were identified using mass GC technology, revealing compounds like 10-Undecyn-1-ol, n-hexadecanoic acid, 4,4-dimethyl-1-hex, and isoamyl nitrite, which were found to have the major function of inhibiting bacterial growth
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Zubair, Faiza, Naila Riaz, and Iqra Khalid. "VALIDATION OF ANTI-BACTERIAL EFFECT OF AMALTAS (Cassia fistula, METHANOLIC EXTRACT) AGAINST SELECTED BACTERIAL STRAINS." Pakistan Journal of Science 74, no. 1-1 (2023): 20–24. http://dx.doi.org/10.57041/pjs.v74i1-1.809.
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Increasing prevalence of antibiotic resistance necessitates the development of more potent natural product that has no adverse effects on human health. Purpose of this study is to verify the medicinal efficacy of selected plant extract (Cassia fistula) against selected bacterial strains. For this purpose the methanolic extract of Cassia fistula (Amaltas) was employed at different concentration, temperature and pH conditions to study its antimicrobial properties against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Bacillus cereus. Results revealed that Cassia fistula showed maximum zone of inhibition (2.6mm±1.1) at 250mg/ml against Bacillus subtilis, while minimum zone (0.6mm±0.00) was observed against Staphylococcus aureus at 150mg/ml. The results for varying pH conditions reveled that Cassia fistula exhibits maximum inhibition (1.5mm±0.2) of Bacillus subtilis at pH-9 (concentration: 150mg/ml). In comparison, at 250mg/ml the observed zone measurement was maximum against Staphylococcus aureus at pH-9. The results at different temperature ranges depict that Cassia fistula effectively controls the growth of Bacillus subtilis at 120 o C (250mg/ml, zone of inhibition: 1.7mm±0.2). All the zones of inhibition were found significant according to the One way ANOVA. This results of current study strengthens the fact regarding antibacterial potential of plant extracts and proves that increasing incidence of antibiotic resistance can be reduced by introducing more natural antibacterial agents other than chemicals.
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Rompas, Widya Fransiska, Defny Silvia Wewengkang, and Deby Afriani Mpila. "ANTIBACTERIAL ACTIVITY TEST OF LAMELLODYSIDEA HERBACEA SPONGE EXTRACT FROM THE WATERS OF POOPOH VILLAGE, MINAHASA REGENCY." PHARMACON 12, no. 3 (2023): 295–301. http://dx.doi.org/10.35799/pha.12.2023.48915.
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Infectious disease is one of the diseases that is currently became a health problem in the community. Along with the increase in cases of infection, there is also an increase in cases of resistance to antibiotics. The biodiversity of Indonesia exhibit a potential at anti-bacterial which could be develop to become antibioctics candidate. This study aims to determine the antibacterial activity of Lamellodysidea herbacea sponge obtained from the waters of Poopoh village, Minahasa Regency against the growth of Staphylococcus aureus and Pseudomonas aeruginosa bacteria. Extraction was carried by maceration out using 95% ethanol solvent. Anti-bacterial testing using agar diffusion method (Disc diffusion Kirby and Bauer). The results showed that the ethanol extracts of Lamellodysidea herbacea sponge had the largest inhibition zone on Staphylococcus aureus bacteria at a concentration of 250 μL/disc of 7.5 mm and Pseudomonas aeruginosa bacteria at a concentration of 250 μL/disc of 7.33 mm, both inhibition zones were in the medium category. Based on this study, it can be concluded that the largest inhibition zone is found in Staphylococcus aureus bacteria.
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Utomo, Feriandri, and Laura Nurul Alfiola. "Pengaruh Ekstrak Etanol 96% Bawang Dayak (Eleutherine Palmifolia (L.) Merr.) Terhadap Pertumbuhan Methicillin Resistant Staphylococcus Aureus (MRSA)." Jurnal Kesehatan Islam : Islamic Health Journal 11, no. 1 (2022): 23–29. http://dx.doi.org/10.33474/jki.v11i1.16102.
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The number of Methicillin Resistant Staphylococcus aureus (MRSA) continues to increase.The antibiotics used to treat MRSA infections is vancomycin, but the number of Staphylococcius aureus bacteria that are resistant to vancomycin has also increased. Dayak onion as a medicinal plant which has been used for a long time, has antibacterial compounds namely flavonoids, tannins, terpenoids, and quinones.The purpose of this study was to analyze the effect of 96% ethanol extract of Dayak onion with concentrations of 12.5%, 25%, 50% on the growth of MRSA compared to vancomycin, and to determine the phytochemical content of MRSA antibacterial compounds in Dayak onions.The study design used a post test only with control group by giving 0.9% NaCl to the negative control group. MRSA growth in this study was assessed from the size of the inhibition zone by the Kirby-Bauer method. Data analysis using One-Way ANOVA , then continued post hoc test. The results of this study indicated that Dayak Onion had weak anti-bacterial activity against MRSA, so it is not effective as an anti-bacterial MRSA because an average inhibition zone of <15mm is formed in all concentration groups (10.88mm at 12,5 % concentration, 12.35mm at 25 % concentration, and 14.35mm at 50% concentration). The average diameter of the inhibition zone in the treatment group of Dayak onion extract with 12,5 % concentration was significantly lower (p value <0.05) than the average diameter of the inhibition zone in the treatment group with 50 % concentration of the Dayak onion extract. The average diameter of the inhibitory zone in the treatment group of all concentrations of Dayak onion extract was significantly lower (p value <0.05) than the average diameter of the inhibition zone in the Vancomycin treatment group. The average inhibition zone formed in the vancomycin treatment group was 22.29 mm, so it was still effective as an anti-bacterial MRSA. This study also found the presence of flavonoid compounds, terpenoids, tannins, and quinones in Dayak onions. Therefore, it can be concluded that the 96% ethanol extract of Dayak onion which contains phytochemical anti-bacterial compounds has weak activity in inhibiting the growth of MRSA, so it is not effective as anti-bacterial for MRSA.
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Muiz, Habib Abdul, Shinta Wulandari, and Annisa Primadiamanti. "UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN PATIKAN KEBO (Euphorbia hirta L) TERHADAP Staphylococcus aureus dengan METODE DIFUSI CAKRAM." Jurnal Analis Farmasi 6, no. 2 (2022): 84–89. http://dx.doi.org/10.33024/jaf.v6i2.5942.
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Patikan kebo (Euphorbia hirta L) is a grass-shaped plant and is often used as a traditional medicine by the community. The people of Pasir Sakti use patikan kebo as a medicine for skin infections. Skin infection is the most common disease caused by Staphylococcus aureus bacteria. Based on research by Harlis 2010 patikan kebo leaves has active compounds of flavonoids and tannins that can inhibit bacterial growth. This study aimed to examine the potential of patikan kebo leaf extract in inhibiting the growth of Staphylococcus aureus bacteria using variants of the concentration of patikan kebo leaf extract, namely 30%, 40%, 50%, and 60%. Tetracyline as positive control and sterile distilled water as negative control. Antibacterial activity test was carried out by disc diffusion method. Based on the results of the inhibition test with three repetitions, the consentration of 30% has inhibition zone of 10.48 mm, the concentration of 40% has inhibition zone 12.26 mm, the concentration of 50% has inhibition zone 14.32 mm, and the concentration of 60% has inhibition zone of 15.26 mm. The conclusion of this study, patikan kebo leaf extract has antibacterial activity against the growth of Staphylococcus aureus bacteria.
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Junie, Lia M., Mihaela L. Vică, Mirel Glevitzky, and Horea V. Matei. "Physico-chemical characterization and antibacterial activity of different types of honey tested on strains isolated from hospitalized patients." Journal of Apicultural Science 60, no. 1 (2016): 5–18. http://dx.doi.org/10.1515/jas-2016-0013.
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AbstractThe first aim of the study was to compare the antibacterial activity of several types of honey of different origins, against some bacterial resistant strains. The strains had been isolated from patients. The second aim was to discover the correlations between the antibacterial character of honey and the physico-chemical properties of the honey. Ten honey samples (polyfloral, linden, acacia, manna, and sunflower) from the centre of Romania were tested to determine their antibacterial properties against the following bacterial species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella enterica serovar Typhimurium, Bacillus cereus, Bacillus subtilis, and Listeria monocytogenes. Bacterial cultures in nutrient broth and the culture medium Mueller-Hinton agar were used. The susceptibility to antibiotics was performed using the disk diffusion method. All honey samples showed antibacterial activity on the isolated bacterial strains, in particular polyfloral (inhibition zone 13-21 mm in diameter) - because it is the source of several plants, and manna (inhibition zone 13-19.5 mm in diameter), and sunflower (inhibition zone 14-18.5 mm in diameter). Pure honey has a significant antibacterial activity against some bacteria which are resistant to antibiotics. Bacterial strains differed in their sensitivity to honeys. Pseudomonas aeruginosa and Staphylococcus aureus were the most sensitive. The present study revealed that honey antibacterial activity depends on the origin of the honey. We also found that there was a significant correlation between antibacterial activity of honeys and the colour of the honey but not between acidity and pH. The statistical analysis showed that the honey type influences the antibacterial activity (diameter of the bacterial strains inhibition zones).
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Mirnawati, Mirnawati, Rasyidah, Ulfayani Mayasari, and Endang Sulistyarini Gultom. "Screening of Extract Secondary Metabolites of Bacteria Which Have Symbiosis with Sponges from Central Tapanuli Bakar Island as an Antibacteria." International Journal of Ecophysiology 6, no. 2 (2024): 35–41. http://dx.doi.org/10.32734/ijoep.v6i1.17821.
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This study aims to determine the presence of antibacterial-producing bacteria from sponges from Bakar Island, Central Tapanuli Regency and to determine the antibacterial activity of methanol extracts from potential bacterial isolates against Staphylococcus aureus and Escherichiacoli. 21 pure isolates were found and it was known that 9 isolates produced antibacterial against S. aureus and 4 isolates against E. coli. Bacterial isolates with the highest inhibition zone against bothS.aureus and E. coli were found in isolates with code SP20 with each large inhibition zone are 14.5 mm and 16.04 mm. Then microscopic observations were carried out, namely gram staining and were obtained 8 isolates were gram positive, and 1 gram was negative. The biochemical test showed that SP20 showed positive results for the Motility, TSIA and Catalase Test but negative for Citrate Test. Bacterial isolates withthe code SP20 had the largest inhibition zone so that these isolates were to be extracted.The resultsof the screening test showed that the secondary metabolitesof the sponge symbiont bacterial extract from Bakar Island were positive for flavonoids and saponins. And then test the activity of secondary metabolites with 3 concentrations, 10%, 20%, and 30%.The results showed that the best concentration of the test bacteria was S. aureus at a concentration of 30% with an inhibition zone of 3.08 mm and E. coli at 5.04 mm.
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Arafah, Annisa Fajriatul, Vivi Triana, and Murniwati Murniwati. "Uji Efektivitas Ekstrak Buah Jeruk Nipis (Citrus aurantifolia) dalam Menghambat Pertumbuhan Bakteri Enterococcus faecalis Secara In Vitro." Andalas Dental Journal 3, no. 2 (2015): 111–18. http://dx.doi.org/10.25077/adj.v3i2.109.
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Enterococcus faecalis is a normal microorganism in root canal teeth, anaerobic facultative, and gram positive cocci. This bacteria is opportunistic that cause secunder infection in root canal. Lime (Citrus aurantifolia) is one of the plants which have antimicrobial activity because lime contains flavonoid and citric acid. Flavonoids can denature proteins in bacterial cell and damaging the cell membrane. Citric acid can damaging the bacterial cell wall and inhibiting bacterial enzymes activity. The purpose of this study was to examie the effectiveness lime extract in various concentration in inhibiting Enterococcus faecalis in in-vitro. The research method was experimental laboratories with posttest only control group design. Samples were used in this research are Enterococcus faecalis bacteria in M. Djamil’s Hospital Microbiology Laboratory, Padang. This research was conducted at organic chemistry of natural materials laboratory FMIPA UNAND and Kopertis Wilayah X Padang laboratory. Early stage was done by making extracts of lime with concentrations are 25%, 50%, 75%, and 100%. Then, the extracts was tested into the bacteria with the diffusion method used papper disk. The inhibition zone formed between lime extracts in various concentrations to the growth of Enterococcus faecalis were calculated using calipers. Data was analyzed by using Kruskal Wallis test and Mann-Whithney test with confidence interval of 95%. The result presented that 25%, 50%, 75%, and 100% lime extracts have inhibition activity in inhibiting the growth of Enterococcus faecalis, and the higher the concentration of the extracts, the greater the inhibition zone formed.
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Baluhya, Marco, and Charles O. Joseph. "Bacterial growth inhibitory effects of Calotropis procera’s latex, leaves and roots extracts." Tanzania Journal of Science 47, no. 2 (2021): 648–54. http://dx.doi.org/10.4314/tjs.v47i2.20.
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This study was conducted to evaluate in vitro antibacterial potential of water and ethanolic extracts from Calotropis procera roots, leaves and latex. The growth inhibitory effects of the extracts were tested against Escherichia coli using disc diffusion method. Amoxicillin was used as positive control, whereas ethanol and distilled water were used as negative controls. Results showed that water extracts from root, latex and leaves had 3.27 ± 0.14, 3.1 ± 0.21 and 2.38 ± 0.24 cm of maximum inhibition zones, while the ethanolic extracts showed 2.91 ± 0.19, 3.02 ± 0.16 and 0.84 ± 0.31 cm, respectively of maximum inhibition zones. The positive control; amoxicillin had 3.56 ± 0.09 cm of maximum inhibition zone. Post hoc tests revealed that roots, latex and conventional amoxicillin were equally effective in inhibiting E. coli growth. It further revealed that water extracts exhibited more growth inhibitory effects than ethanol extracts. No significant variations were observed on the E. coli growth inhibition due to the locations from where C. procera were collected. Generally, results indicated that C. procera inhibited the growth of E. coli. Thus its uses for the development of traditional and complementary medicines and further investigation on its biological activity on other microbes are highly recommended.
 Keywords: Antibacterial, Calotropis procera, Diameter of inhibition zone. Escherichia coli, Plant crude extract, Traditional medicine.
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Handayani, Muliawati, Muhammad Arif Asadi, and Oktiyas Muzaky Lutfi. "Assesment Zona Inhibisi Bakteri Endosimbion Karang Keras Porites sp. di Selat Sempu, Malang Selatan." Journal of Marine Research 13, no. 4 (2024): 635–42. http://dx.doi.org/10.14710/jmr.v13i4.44830.
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White Syndrome (WS) merupakan salah satu jenis coral desease yang ditemukan di ekosistem terumbu karang di Selat Sempu, Malang Selatan. Pada umumnya WS menginfeksi karang massive, salah satunya adalah Porites sp. yang merupakan spesies karang terbanyak di perairan ini. Tujuan penelitian ini adalah untuk menganalisa zona hambat/ inhibisi yang terbentuk dari uji tantang bakteri endosimbion karang Porites sp. yang terinfeksi WS dengan bakteri endosimbion karang sehat. Pengambilan sampel dilakukan secara purposive sampling. Pendekatan mikrobiologi digunakan untuk mendapatkan kultur murni dari isolat bakteri. Kemudian, uji tantang dilakukan dengan metode overlay menggunakan difusi cakram antara bakteri endosimbion yang memiliki potensi antibakteri terhadap WS. Zona inhibisi akan terbentuk ketika bakteri dari karang sehat memiliki antibody untuk melawan bakteri penyebab karang sakit. Pasangan bakteri yang membentuk zona inhibisi terbesar berhasil identifikasi secara molekuler. Hasil BLAST isolat bakteri endosimbion pada karang sehat merujuk pada bakteri Marinobacter vinifirmus yang mampu menghambat isolat bakteri Altererhytrobacter sp. dengan zona inhibisi 6 mm. White Syndrome (WS) is one of the coral diseases found in the coral ecosystem in the Sempu Strait, South Malang. Porites sp. was dominant species in these waters infected WS. This study aims to analyze inhibition zones formed overlay of endosymbiont bacteria Porites sp. which are infected by WS and healthy endosymbiont coral bacteria. Specimen was collected by purposive sampling. The microbiological approach was used to obtain pure cultures from bacterial isolates. Then, challenge is using the overlay method. The inhibition zone formed when bacteria from healthy corals have antibodies to fight the bacteria that cause WS. The largest inhibition zone (6 mm) succeeded in molecular identification. The results of BLAST endosymbiont bacteria on healthy corals refers to Marinobacter vinifirmus,which is able to inhibit bacterial of Altererhytrobacter sp.
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Farooqui, Mazahar, Nasir Ali Shafakat Ali, Zaheer Zaheer, Zia Ur Rahman, and Ashpaq Khan. "Synthesis, Anti-bacterial, Analgesic and Anti-inflammatory Activities of Some New Biologically Important Indazole Derivatives." International Journal of Drug Design and Discovery 1, no. 4 (2024): 314–24. https://doi.org/10.37285/ijddd.1.4.5.
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A series of new indazolyl derivatives (1.1A - 1.5E) have been prepared from commercially available N-methyl-3-indazole carboxylic acid and 7-amino cephalosporic acid. The synthesized compounds were evaluated for anti-bacterial, analgesic and in vivo anti-inflammatory activities using acetic acid writhing in mice and carrageenan paw oedema method in mice. The series of compounds have analgesic activity in the order 1.4D>1.3C>1.5E>1.2B >1.1A Compound 1.3C had maximum zone of inhibition for Bacillus thurengensis, Staphylococus aureus, Bacillus Subtilis and showed least zone of inhibition to E. coli. It did not show any zone of inhibition to remaining bacteria. Compound 1.2B showed maximum zone of inhibition to Bacillus thurengensis and E. coli. Compound 1.1A showed maximum zone of inhibition Proteus vulgaris Bacillus Subtilis and Bacillus thurengensis
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Regitha Adiba Fayza Purwoko, Riana Putri Rahmawati, and Muhammad Khudzaifi. "Pengaruh Antibakteri Kombinasi Ekstrak Kulit Jeruk Manis (Citrus Sinensis (L.) Osbeck) dan Daun Pepaya (Carica Papaya L.) Terhadap Bakteri Escherichia Coli." Jurnal Ilmu Kedokteran dan Kesehatan Indonesia 5, no. 2 (2025): 124–47. https://doi.org/10.55606/jikki.v5i2.6079.
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Sweet orange peel and papaya leaves have been used by the community to prevent and treat various diseases caused by bacterial, fungal, and viral infections. Sweet orange peel contains flavonoids, alkaloids, saponins, tannins, and terpenoids that can inhibit bacterial growth. While papaya leaves contain active compounds such as alkaloids, flavonoids, steroids, tannins, and terpenoids that can inhibit bacterial growth. Escherichia coli bacteria are gram-negative, facultative anaerobic bacteria that can cause intestinal infections, causing diarrhea. The combination of extracts from several plants has greater inhibitory power so that it has greater antibacterial activity than a single plant. The purpose of this study was to determine whether the combination of sweet orange peel and papaya leaf extracts has an effect on the growth of Escherichia coli bacteria. The test method used was the disc diffusion method on TSA agar media. Sweet orange peel and papaya leaf extracts in this study were obtained by the maceration method with 96% ethanol solvent. The ratio of extracts used was sweet orange peel and papaya leaves 1:3; 2:2; 3:1. The positive control used was ciprofloxacin. The negative control used was 10% dimethyl sulfoxide (DMSO). The results of this study indicate that the combination of sweet orange peel extract (Citrus sinensis L. Osbeck) and papaya leaves (Carica papaya L.) has an effect on the growth of Escherichia coli bacteria because it produces an inhibition zone around the disc paper. All comparisons, namely 1: 3; 2: 2; 3: 1 are optimal in inhibiting the growth of Escherichia coli bacteria with a diameter of the inhibition zone in sequence of 17.78 mm (strong category), 22.14 mm (very strong category), and 26.28 mm (very strong category). Likewise, the positive control produces an inhibition zone of 34.25 (very strong category). The most optimum antibacterial combination shows the diameter of the inhibition zone against Escherichia coli bacteria, namely at a ratio of 3: 1.
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Razon, Brando Cabigas. "Soft Coral Epibiotic Bacteria Vibrio alginolyticus from Sarangani Bay, Mindanao Philippines as Potent Source of Antibacterial Agent." Indonesian Journal of Science and Technology 9, no. 3 (2024): 747–58. https://doi.org/10.17509/ijost.v9i3.74690.
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This study aims to explore the antibacterial properties of the different epibiotic bacterial species thriving in the surface tissues of the soft corals, and their potential application in developing novel antibacterial agents. There were ten soft coral species randomly collected from Sarangani Bay in Mindanao, Philippines: 3 sarcophyton sp., two sinularia sp., 1 nepthia sp., 1 stenonephthya sp., 1 anthelia sp., 1 lithophyton sp. and 1 asteropicularia sp. All soft coral species collected harbored epibiotic bacteria. Fifteen epibiotic bacterial species were isolated and tested for antibacterial properties against Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) as test organisms. Their respective zones of inhibition were compared to that of the commercial antibiotics Penicillin, Chloramphenicol, and Gentamycin (as positive controls). Results indicated varied antibacterial effects, with one isolate, Vibrio alginolyticus (EB6), showing significant activity, with the zone of inhibition of 9.33 mm against E. coli, comparable to that of Chloramphenicol and Gentamycin, with the zone of inhibition of 10 and 9 mm, respectively. These findings highlight the potential of marine-derived bacteria in developing new antibacterial agents.
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Aini, Nur, and Suharjono Suharjono. "Diversity and Potential Analysis of Liquid Biopesticide Bacteria for Fusarium sp. Control of Shallot -Basal Rot." Biotropika: Journal of Tropical Biology 12, no. 1 (2024): 16–25. http://dx.doi.org/10.21776/ub.biotropika.2024.012.01.03.
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Shallot base rot disease (Allium cepa L.) caused by the fungus Fusarium causes many crop losses, thus control efforts are required. Microbial biopesticides are used as an alternative to control pests and plant pathogens that are environmentally friendly and target-specific. This study aims to evaluate the diversity of liquid biopesticide bacteria and their potential to control the Fusarium sp. pathogen of shallots. This study includes shallot sampling, isolation and purification of liquid biopesticide bacteria, liquid biopesticide antifungal test, double culture antagonistic test of bacterial isolates against Fusarium sp., and identification of potential bacterial isolates based on 16S rDNA sequence similarity. The results showed that 2 bacterial isolates B7 and B2 had the highest Importance Value Index of 94.21% and 90.59% respectively, with a bacterial diversity index of 1.44. Liquid biopesticide microbial biomass has a high inhibition zone in the antifungal test against Fusarium sp. with an average inhibition zone of 6.45 mm. Bacterial isolate B2 has the highest potential inhibition of 71.3% against Fusarium sp. and was identified as Genus Serratia with a similarity value of 96.79%.
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Oraibi, Asma G., Rana A. Mohsien, and Mallic Habeeb. "Antibacterial activity of copper nanoprticles and Watercress (Nasturtium officinale R. Br.) extracts." Asian-Australasian Journal of Bioscience and Biotechnology 1, no. 3 (2016): 564–71. http://dx.doi.org/10.3329/aajbb.v1i3.64039.
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This study was aimed to detect the antibacterial activity of N. officinale leaves water and ethanol extract alone or mixed with a specific concentrations of copper nanoparticles (800mg/L). Studied Bacterial samples consist of two gram-positive bacteria (Escherichia coli and Pseudomonas aeruginosa) and two gram-negative bacteria (Staphylococcus aureus and Streptococcus pyogenes). Results showed that there was a significant increase in the mean inhibition zone at 1:1 (v/v) of 1.0mg/10ml copper nanoparticles and water leaves extract (J) and 1:1 (v/v) of 0.5mg/10ml mg/10ml copper nanoparticles and ethanol leaves extract (F) compared with 0.5mg/10ml leaves water extract (B) and 0.5mg/10ml leaves ethanol extract (C), but there was no significant differences obtained between the types of treatments and the standard antibiotic that used as control (250 mg/10ml Cefotaxime), the highest mean inhibition zone was recorded in Staphylococcus aureus bacteria compared with other bacterial species. The interaction revealed that the highest mean inhibition zone obtained in 1:1 (v/v) of 1.0mg/10ml copper nanoparticles and water leaves extract (J) in Staphylococcus aureus bacteria and the lowest mean inhibition zone observed in 1.0mg/10ml copper nanoparticles (I) in Pseudomonas aeruginosa bacteria while mean inhibition zone significantly increased in E. coli bacteria at 1:1 (v/v) of 0.5mg/10ml mg/10ml copper nanoparticles and ethanol leaves extract (F), 1:1 (v/v) of 1.0mg/10ml copper nanoparticles and water leaves extract (J), and 1:1 (v/v) of 1.0mg/10ml copper nanoparticles and ethanol leaves extract (K). No significant differences recorded in the mean inhibition zone of Pseudomonas aeruginosa and Staphylococcus aureus bacteria in various treatments. Asian Australas. J. Biosci. Biotechnol. 2016, 1 (3), 564-571
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‘Adawiyah, Robi’atul, Dadan Ramadhan Apriyanto, and Rama Samara Brajawikalpa. "Phytochemical Screening and Potency of Mango Peel Extract (Mangifera indica L.) var. Gedong Gincu in Inhibiting the Growth of Staphylococcus aureus." GHMJ (Global Health Management Journal) 8, no. 1s (2025): 74–83. https://doi.org/10.35898/ghmj-81s1156.
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Background: Staphylococcus aureus is a pathogen most of which develop into Methicillin-resistant Staphylococcus aureus. To prevent bacterial resistance, herbal medicine is needed. Mango plants have secondary metabolite compounds that can inhibit bacterial growth. Gedong gincu mango is a specific mango variety that grows widely in Cirebon district. There has been no research that knows the secondary metabolite content and its potential as an antibacterial, especially the peel part which only becomes waste. Aims: To find out the chemical compounds contained and determine the potential of mango peel extract (Mangifera indica L.) var. gedong gincu in inhibiting the growth of Staphylococcus aureus. Methods: This research is an experimental with a posttest only control group design. Phytochemical screening test employed a qualitative method. The extract was made using the maceration method with 70% ethanol solvent. Antibacterial testing with well diffusion method, and given four treatment concentrations (W/V), namely 25%, 50%, 75%, 100%. The measurement on the inhibitory zone after 24 hours at temperature of 370C. Results: Gedong gincu mango peel extract contains secondary metabolite compounds flavonoids, tannins, saponins, and steroids. The inhibitory activity of gedong gincu mango peel extract with a concentration of 25% gedong gincu mango peel extract has an average inhibition zone of 11,55 mm, 50% average inhibition zone 13,55 mm, 75% average inhibition zone 14,88 mm, and 100% average inhibition zone 16,22 mm in inhibiting the growth of Staphylococcus aureus p(<0.05). Conclusion: Mango peel extract var. gedong gincu with a concentration of 25% has the potential to inhibit the growth of Staphylococcus aureus bacteria.
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Akhyar, Halil, Selvira Anandia Intan Maulidya, Muhammad Mukaddam Alaydrus, et al. "Studi Pemodelan dan Prediksi Aktivitas Antibakteri Biopo-limer Kitosan Menggunakan Response Surface Methodology (RSM)." JTIM : Jurnal Teknologi Informasi dan Multimedia 7, no. 2 (2025): 352–62. https://doi.org/10.35746/jtim.v7i2.713.
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Infections occured in the human are mostly caused by uncontrolled growth of Staphylococcus aureus bacteria. A strategy to inhibit bacterial growth can use antibacterial agents such as chitosan. The mechanism of the effectiveness of chitosan as an antibacterial is quite complex, even the data on its antibacterial activity is quite fluctuating so that it is difficult to analyze accurately and efficiently. Therefore, the purpose of the study was to predict the inhibition zone of s.aureus bacteria through laboratory experiments combined with modeling using the Central Composite Design (CCD) approach. The research was carried out with two main stages, including chitosan isolation and calculation of bacterial inhibition zones. The production of chitosan leverages the microwave isolation and FTIR to examine for the degree of deacetylation and its functional group using. Furthermore, the antibacterial activity of chitosan biopolymer was tested using the diffusion method combined with modeling using the RSM CCD approach. The results showed that chitosam from oyster shell was obtained by DD of 83.29% and the emergence of typical chitosan groups, such as amine (NH2) and hydroxyl (OH). Chitosan can hamper the growth of s. aureus bacteria with an inhibition zone of up to 0.40 mm. The experimental data were combined with computational modeling obtained the values of the determination coefficient R2 = 0.6083. The modeling was assessed by p-value of < 0.0001 and F-value of 13.46. Statistically, the obtained model is relevant to the relationship between the number of bacterial colonies and the concentration of chitosan solution with the bacterial inhibition zone. Based on numerical analysis and modeling, the predicted values of the number of s. aureus bacterial colonies and chitosan concentrations were 550,000 CFU/ml and 42.5%. Therefore, Pearl shells can be isolated into chitosan, as well as chitosan has the potential to be a good antibacterial agent. The model has good prediction performance, but it rquires to increase the number of point spreads and it is necessary to validate the prediction results to obtain actual predictions.
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Baba, Firda, Marzelina Karim, Andi Dhedie Prasatia Sam, Yani Sodiqah, and Dhian Karina Hatta. "Effectiveness Test of Saliara Leaf Extract (Lantana camara) on Escherichia coli Bacteria Kirby Bauer Disc Method." Jurnal Biologi Tropis 24, no. 3 (2024): 1–5. http://dx.doi.org/10.29303/jbt.v24i3.7294.
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Death due to infection is the most common cause and is a challenge in the health sector. In 2019, 7.7 deaths were due to bacterial infections, 90 to 100 cases were urinary tract infections and Escherichia coli bacteria contributed to 90% of urinary tract infections. This research uses the Kirby Bauer Disc method to determine the effectiveness of Saliara (Lantana camara) leaves against Escherichia coli bacteria. The type of research is a Laboratory experiment using the Kirby Bauer disc method with a post-test-only control group design. Data analysis uses recording of identifying the inhibition zones formed The research showed that a sample's concentration can influence the inhibition zone's resulting diameter. Saliara leaf extract with concentrations of 100%, 150%, and 200% produced an inhibition zone of 8.55 mm; 9.59mm; 12.34 mm in the proliferation of Escherichia coli bacteria. The positive control with the antibiotic ciprofloxacin produced an inhibition zone of 19.56 mm on the growth of Escherichia coli bacteria. Control with negative distilled water does not create an inhibition zone because it does not have antibacterial activity. The conclusion is moderate inhibitory activity in Saliara (Lantana camara L.) leaf extract at concentrations of 100% and 150%. It is strong at a concentration of 200% against the growth of Escherichia coli bacteria.
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Hainil, Sri, Suci Fitriani Sammulia, and Adella Adella. "Aktivitas Antibakteri Staphyloccocos aureus dan Salmonella thypi Ekstrak Metanol Anggur Laut (Caulerpa racemosa)." Jurnal Surya Medika 7, no. 2 (2022): 86–95. http://dx.doi.org/10.33084/jsm.v7i2.3210.
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Sea Grape is one type of marine plant that produces active compounds that have the potential as medicinal ingredients that have antibacterial properties to inhibit the growth of bacteria that cause infectious diseases. This study aims to determine the antibacterial activity of sea grapes from Kampung Terih, Nongsa Beach, Riau Islands. The bacterial test used was Staphylococcus aureus and Salmonella typhi. Antibacterial activity testing using the disc paper diffusion method was carried out by measuring the inhibition zone around the disc paper, using a concentration variant of 25%, 75%, 100%. The results showed that sea grape extract was able to inhibit the growth of Staphylococcus aureus bacteria by forming an average diameter at each concentration of 8.4 mm (medium inhibition); 9.1 mm (medium resistance); 15.7 mm (strong resistance); then Salmonella typhi bacteria formed an average diameter at each concentration of 8.3 mm (medium inhibition); 9.3 mm (medium inhibition); 19.9 mm (strong resistance). Sea grape extract has strong potential in inhibiting bacterial growth.
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Admassie, Mesele, Yitbarek Woldehawariat, and Tesfaye Alemu. "In Vitro Evaluation of Extracellular Enzyme Activity and Its Biocontrol Efficacy of Bacterial Isolates from Pepper Plants for the Management of Phytophthora capsici." BioMed Research International 2022 (September 26, 2022): 1–13. http://dx.doi.org/10.1155/2022/6778352.
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Phytophthora capsici is one of the most devastating fungal pathogens, causing severe diseases that lead to economic loss in the pepper industry. As a result of the infections, the chemical approach is becoming more popular. Biological control, on the other hand, is better suited to controlling fungal pathogens. The biological control approach significantly reduces the problems associated with chemical applications while restoring natural environmental balance. As a result, the overall findings indicate that certain bacterial isolates play a beneficial role in lytic enzyme production and biocontrol activities against P. capsici. Bacterial isolates obtained from the pepper plants were screened for lytic enzyme and anti-oomycete activity against Phytophthora capsici in Ethiopia. Sixty bacterial isolates were isolated and tested against Phytophthora capsici. From these bacterial isolates, different inhibition zones and hydrolytic enzyme production were detected. Biochemical tests using an automated machine (MALDI-TOF, VITEK 2 compact and 16S rRNA) revealed that three of them, AAUSR23, AAULE41, and AAULE51, showed a high inhibition zone and high production of hydrolytic enzymes and were identified as Enterobacter cloacae (AAUSR23), Pseudomonas fluorescens (AAULE41), and undetermined (AAULE51). The effects of diffusable metabolite isolate AAULE51 has a 66.7% inhibition zone against Phytophthora capsici, followed by AAULE41 and AAUSR23, which have 59.7% and 14.1% inhibition zones, respectively. These bacterial isolates showed high production of hydrolytic enzymes like protease, cellulase, chitinase, and lipase (5-34 diameter of inhibition zone). As a result, the overall findings show that selected bacterial isolates play a beneficial role in lytic enzyme production and for their biocontrol activities against P. capsici.
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Aliah, Ahmad Irsyad, Wahyuni Wahyuni, and Nurjannah Bachri. "UJI DAYA HAMBAT FORMULA GEL EKSTRAK ETANOL DAUN MURBEI (Morus alba L.) SEBAGAI ANTI ACNE TERHADAP BAKTERI Propionibacterium acne." Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) 5, no. 2 (2019): 206. http://dx.doi.org/10.22487/j24428744.0.v0.i0.13663.
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A research on the inhibition of formula gel test ethanol extract of leaves of mulberry (Morus alba L.) as an anti-acne against bacteria Propionibacterium acne. This study uses a gel formulation made using mulberry leaf extract (Morus alba L.), which aims to determine the inhibition of antibacterial gel mulberry leaf extract (Morus alba L.) against the bacteria Propionibacterium acne. Gel made three dosage concentrations of 2%, 4% and 6%. Testing inhibition of antibacterial gel agar diffusion method. Test Antibacterial inhibition obtained by looking at the clear zone in the medium and measured using calipers. The results showed that the inhibition of antibacterial gel formulation with a concentration of 2%, 4% and 6% showing that inhibiting bacterial activity.
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Aliah, Ahmad Irsyad, Wahyuni Wahyuni, and Nurjannah Bachri. "Uji Daya Hambat Formula Gel Ekstrak Etanol Daun Murbei (Morus alba L.) Sebagai Anti Acne Terhadap Bakteri Propionibacterium acne." Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) 5, no. 2 (2019): 206–13. http://dx.doi.org/10.22487/j24428744.2019.v5.i2.13663.
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A research on the inhibition of formula gel test ethanol extract of leaves of mulberry (Morus alba L.) as an anti-acne against bacteria Propionibacterium acne. This study uses a gel formulation made using mulberry leaf extract (Morus alba L.), which aims to determine the inhibition of antibacterial gel mulberry leaf extract (Morus alba L.) against the bacteria Propionibacterium acne. Gel made three dosage concentrations of 2%, 4% and 6%. Testing inhibition of antibacterial gel agar diffusion method. Test Antibacterial inhibition obtained by looking at the clear zone in the medium and measured using calipers. The results showed that the inhibition of antibacterial gel formulation with a concentration of 2%, 4% and 6% showing that inhibiting bacterial activity.
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Budi, Andre, and Novita Lina Sembiring. "POLARESISTENCY OF SALMONELLA TYPHI BACTERIA TO ANTIBIOTIC CEFTRIAXONE AND CIPROFLOXACIN." Journal Health & Science : Gorontalo Journal Health and Science Community 6, no. 2 (2022): 58–67. http://dx.doi.org/10.35971/gojhes.v5i3.13624.
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Typhoid fever is an unbearable severe infection of the small digestive system, with the side effect of fever lasting up to seven days, and accompanied by stomach related disorders. Typhoid fever is a very common disease in Indonesia, both among clinical faculties and the population as a whole, and assuming that a child complains of fever, antimicrobials are the therapy of choice. Inappropriate use of various types of antibiotics, due to easy public access to drugs, will lead to an increase in the incidence of bacteria, including Salmonella typhi, which are resistant to ABRB (Antibiotic Resistant Bacteria) antibiotics. The type of research used in this study was Experimental Laboratory with a research design using the Disc Diffusion Method. This study aims to determine the resistance pattern of Salmonella typhi bacteria to Ceftriaxone and Ciprofloxacin antibiotics. This study aims to determine the resistance pattern of Salmonella typhi bacteria to Ceftriaxone and Ciprofloxacin antibiotics. The average value (mm) for 24 hours of ceftriaxone: 0.35, ciprofloxacin: 29,175 The average value (mm) for 48 hours of ceftriaxone: 14.8, ciprofloxacin: 29.4 Based on the results of the research data and images, it can be concluded that the diameter of the inhibition zone or clear zone of the salmonella typhi bacteria culture contained in petri dishes with MHA media on the ceftriaxone antibiotic was found to be an intermediate, which is a transition state from a drug resistant state but not completely resistant, while the ciprofloxacin antibiotic showed that the results were still sensitive to salmonella typhi bacteria. , with an average value of 14.8 and 29.4 mm where according to the CLSI (Clinical Laboratory Standards Institute) standard, the diameter of the bacterial inhibition zone is 17 mm, the intermediate category if the diameter of the bacterial inhibition zone is 14-16 mm, and the resistant category if the diameter of the bacterial inhibition zone is 14-16 mm. Bacterial inhibition zone is 13 mm.AbstrakDemam tifoid adalah infeksi hebat yang tak tertahankan pada system pencernaan kecil, dengan efek samping demam yang berlangsung hingga tujuh hari, dan disertai gangguan terkait perut. Penggunaan berbagai jenis antibiotik secara luas yang tidaktepat, akibat mudahnya akses masyarakat terhadap obat akan menyebabkan peningkatan kejadian bakteri, termasuk Salmonella typhi, yang resisten terhadap antibiotik ABRB (Antibiotic Resistant Bacteria. Jenis penelitian yang digunakan dalam penelitian ini adalah Eksperimental Laboratorium dengan rancangan penelitian menggunakan Metode Difusi Kertas Cakram (Disc Diffusion Method). Penelitian ini bertujuan untuk mengetahui polaresistensi bakteri Salmonella typhi terhadap antibiotik Ceftriaxone dan Ciprofloxacin.Penelitian ini bertujuan untuk mengetahui polaresistensi bakteri Salmonella typhi terhadap antibiotik Ceftriaxone dan Ciprofloxacin. Nilai rata-rata (mm) selama 24 jam ceftriaxone: 0,35 , ciprofloxacin : 29,175 Nilai rata-rata (mm) selam 48 jam ceftriaxone : 14,8 , ciprofloxacin : 29,4 Berdasarkan hasil data dan gambar penelitian dapat di simpulkan bahwa diameter zona hambat atau zona bening dari biakan bakteri salmonella typhi yang terdapat dalam cawan petri dengan media MHA pada antibiotik ceftriaxone ditemukan sebagai intermediate yaitu suatu keadaan transisi dari keadaan resisten obat tetapi tidak resisten sepenuhnya, sedangkan pada antibiotik ciprofloxacin menunjukkan hasil masih sensitive terhadap bakteri salmonella typhi , dengan nilai rata-rata 14,8 dan 29,4 mm dimana menurut standart CLSI (Clinical Laboratory Standards Institute), diameter zona hambat bakteri ≥ 17 mm, kategori intermediet apabila diameter zona hambat bakteri 14-16 mm, dan kategori resisten apabila diameter zona hambat bakteri yaitu ≤ 13 mm.
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Naqvi, Syed Ali Raza, Syed Muhammad Ali Shah, Laiba Kanwal, et al. "Antimicrobial and Antihypercholesterolemic Activities of Pulicaria gnaphalodes." Dose-Response 18, no. 1 (2020): 155932582090485. http://dx.doi.org/10.1177/1559325820904858.
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Multidrug resistance has increased globally in the communities. Bacterial infections associated with health care have weakened the existing antimicrobial therapy and demand the search for alternative therapies. In the present investigation, the medicinal plant Pulicaria gnaphalodes from Quetta, Pakistan, has been screened for antimicrobial potential. In vitro antimicrobial efficacy of P gnaphalodes extracts (methanol and ethanol) was quantitatively evaluated on the basis of zone of inhibition against different bacteria and minimum inhibitory concentration (MIC). In vivo, antihypercholesterolemic activity is determined in different rat groups. The results of the study indicated that the ethanol extract of P gnaphalodes showed maximum zone of inhibition for Bacillus subtilis of 12.1 ± 1.1 mm from all others. The methanol extract showed maximum zone of inhibition for Staphylococcus aureus of 11.9 ± 1.0 mm and rifampicin showed maximum zone of inhibition of 23.1 ± 0.9 mm. The results of ethanol and methanol extract of P gnaphalodes against different bacteria revealed that this plant has greater antimicrobial activity. However, the plant extract shows nonsignificant antihypercholesterolemic activity. The extract of this plant can be utilized as medicine to inhibit several infections caused by some bacterial pathogens found in human body.
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GOPİNATH, Nagomi. "Antimicrobial potential of lemon and onion extracts against gram-positive and -negative bacteria." International Journal of Secondary Metabolite 10, no. 4 (2023): 626–37. http://dx.doi.org/10.21448/ijsm.1290157.
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Antimicrobial potentials have been widely analyzed with different sources; however, plant-based antimicrobial compounds are greatly welcome due to their greener characteristics. This study revealed the importance of antimicrobial compounds from the herbal extracts of lemon and onion. The extracts were tested against gram-negative (Escherichia coli) and gram-positive (Bacillus subtitlis) bacteria. Disc-diffusion and well-diffusion on an agar plate and tributary methods were followed to demonstrate the antimicrobial potentials of the above herbal extracts. Further, different volumes of ampicillin at the concentration of 1 mg/ml were used to compare the genuine bacterial inhibition (3 µL with 1.5 cm zone). Lemon behaved excellently in a way by displaying better bacterial inhibition against both E. coli (3 µL with 1.2 cm zone) and B. Subtilis (3 µL with 0.6 cm zone), whereas onion extract was not at the level of lemon extract; however, it still displayed a good inhibition. The turbidity assay confirms the inhibition efficiency of lemon and onion against both E. coli and B. subtilis. In the liquid medium lemon shows higher inhibition (2 & 3 folds) on bacteria than that of ampicillin and onion. Cell count and UV-vis spectroscopy analysis at 600 nm also conform to the efficacy of lemon inhibition against E. coli and B. subtilis. This experiment confirms that lemon extract is an excellent and better substitute for commercially available ampicillin for bacterial inhibition.
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Wanda, Agus Triwanda, Yuni Puji Hastuti, and Sinung Rahardjo. "ANTIBACTERIAL ACTIVITIES OF MANGROVE PLANT EXTRACT (Rhizophora stylosa) TO THE GROWTH OF BACTERIA Vibrio parahaemolyticus." Aurelia Journal 5, no. 1 (2023): 47. https://doi.org/10.15578/aj.v5i1.11650.
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Vibrio parahaemolyticus is a gram-negative halophilic bacterium that lives in estuaries and beaches. These bacteria are agents that cause septicemia in shrimp during the larval and post larvae period. This study aims of this experiment is to determine the effect of mangrove leaf, root, stem and fruit extracts of Rhizophora stylosa growth of the V. parahaemolyticus bacteria. The experimental design used a completely randomized design (CRD) with positive control treatment (Papper disk containing chloramphenicol); Negative control (Papper disk containing methanol) P1 = mangrove extract 100 mg/L; P2 = 200 mg/L mangrove extract; P3 = 400 mg/L mangrove extract and in doing it on 3 times. The research parameter was measuring the bacterial inhibition zone. The results showed that the mangrove leaf, root, stem and fruit extracts of R. stylosa significantly affected on the inhibition zone and of high concentrations of the leaf, root, stem extract concentrations of R. stylosa mangrove fruit, the wider the bacterial inhibition zone. The concentration of 400 mg/L extracts of leaves, roots, stems and fruit heads of R. stylosa mangroves was the most effective concentration to inhibit the growth of V. parahaemolyticus bacteria, which showed an average root inhibition zone of 9.5 mm, leaves 7.7 mm, stems 6.5 mm, and fruit heads 4.8 mm.
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Kartika, Dewi, Romauli Anna Teresia Marbun, and Ayu Puspita Dewi. "ANTIBACTERIAL ACTIVITY OF KERSEN LEAF EXTRACT (MUNTINGIA CALABURA L) AGAINST STAPHYLOCOCCUS AUREUS." JURNAL FARMASIMED (JFM) 4, no. 2 (2022): 59–63. http://dx.doi.org/10.35451/jfm.v4i2.1012.
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Kersen (Muntingia calabura) is a shady plant that is usually used as a shade. Cherry leaves contain several secondary metabolites such as flavonoid, tannin and terpenoid. Flavonoid can inhibit bacterial growth by denaturing proteins and damaging cell membranes and dissolving fats contained in cell walls. According to the World Health Organization as many as 25 million deaths worldwide in 2011, one third were caused by infectious diseases. One of the most common infectious diseases caused by the bacterium Staphyloccus aureus. The purpose of this study was to see whether the 96% ethanol extract of cherry leaves had antibacterial activity against Staphylococcus aureus. The concentration of the extract used was 15%, 30%, 45% and 60%. From the test results, it can be concluded that the largest inhibition zone diameter is at a concentration of 60% with an inhibition zone diameter of 6.78 mm and the smallest at a concentration of 30%, which is 0.42 mm. Concentration of 45% has (weak) activity inhibiting bacterial growth with an inhibition zone of 2.13 mm, while at a concentration of 15% it has no activity in the inhibition zone of bacterial growth.
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Kartika, Dewi, and Shopian Syarifuddin. "SOCIALIZATION OF ANTIBACTERIAL ACTIVITY OF KERSEN LEAF EXTRACT (Muntingia calabura L) AGAINST BACTERIA Staphylococcus aureus." JURNAL PENGMAS KESTRA (JPK) 2, no. 2 (2022): 206–9. http://dx.doi.org/10.35451/jpk.v2i2.1453.
Full textAbstract:
Kersen (Muntingia calabura) is a shady plant that is usually used as a shade. Cherry leaves contain several secondary metabolites such as flavonoid, tannin and terpenoid. Flavonoid can inhibit bacterial growth by denaturing proteins and damaging cell membranes and dissolving fats contained in cell walls. According to the World Health Organization as many as 25 million deaths worldwide in 2011, one third were caused by infectious diseases. One of the most common infectious diseases caused by the bacterium Staphyloccus aureus. This socialization aims to provide information to the public about the antibacterial ability of kersen leaves against staphylococus aureus. The concentration of the extract used was 15%, 30%, 45% and 60% and erythromycin as a positive control. From the test results, it can be concluded that the largest inhibition zone diameter is at a concentration of 60% with an inhibition zone diameter of 6.78 mm and the smallest at a concentration of 30%, which is 0.42 mm. Concentration of 45% has (weak) activity inhibiting bacterial growth with an inhibition zone of 2.13 mm, while at a concentration of 15% it has no activity in the inhibition zone of bacterial growth
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IA Karfi, UU Zango, T Olatinwo, and M Suleiman. "Phytochemical screening and antibacterial activity of Vernonia amygdalina (bitter leaf) on some selected bacterial isolates." International Journal of Biology and Pharmacy Research Archive 1, no. 1 (2021): 006–13. http://dx.doi.org/10.53771/ijbpra.2021.1.1.0013.
Full textAbstract:
Plants with medicinal value produce certain chemical elements known as phytochemicals that have antibacterial activity. The study was aimed at determining the antibacterial activity of Vernonia amygdalina against bacterial isolates using agar well diffusion method. In addition, the phytochemicals analysis of the extracts was also determined. The phytochemical analysis showed the presence of saponins, steroids, terpenoids, tannins, alkaloids, and flavonoids. The result of Vernonia amygdalina showed that the average zones of inhibitions observed against these bacterial ranges from 6-22mm. The highest zone is also exhibited against E. coli with average diameter of zone of inhibition of 22mm. At 100mg/ml concentration for Samonella, the zone of inhibition was recorded to be 21mm while at 12.5mg/ml there was no inhibition. At 25mg/ml and 12.5mg/ml, against Pseudomonas there was no inhibition. In other to further confirm the activity of these plant extracts, the minimum inhibitory concentration and minimum bactericidal concentration was determined and the result showed that the extract exerted good antibacterial activity on all the test organisms at different concentration. The result of minimum inhibitory concentration ranges from 10 to 12.5mg/ml and that of MBC ranges from 5 to 20mg/ml. It is worthy to note that MBC values is greater than that of minimum inhibitory concentration. The study provides insight into the antibacterial activities of the plant extracts and its use in the treatment of bacterial infections.
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Ohemu Godwin Pius and Fajoyomi Bridget Uredo-ojo. "Antimicrobial effect of Jatropha tanjorensis leaves on multi antibiotic resistant bacterial isolated from poultry droppings." World Journal of Advanced Research and Reviews 13, no. 1 (2022): 775–80. http://dx.doi.org/10.30574/wjarr.2022.13.1.0106.
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The widespread use of antibiotics as prophylactics and therapeutic agents has increased the risk of emergence and spread of resistant bacteria leading to prolonged and expensive treatment and greater risk of death in humans. The aim of this study was to determine the antimicrobial effect of the methanol extract of Jatropha tanjorensis leaves on multi antibiotic resistant bacteria isolated from poultry droppings. Multi antibiotic resistant bacterial isolates from poultry dropping already identified from previous research were used. The plant extract was obtained by soxhlet extraction process, susceptibility test was caried out using well in agar diffusion method. The percentage yield of the plant extract using soxhlet extraction process with methanol was 22.84%. The qualitative phytochemical analysis revealed the presence of bioactive compounds such as tannins, flavonoids, cardenolide, steroids, terpenoids, anthraquinone, alkaloids, fixed oil and carbohydrate. The extract showed competitive zones of inhibition on Escherichia fergusonii, Stenotrophomonas maltophilia, Enterobacter clocae, Lysinibacillus sphaericus and Salmonella typhimerium. Enterobacter clocae had the highest zone of inhibition of 17mm at a concentration of 100mg/ml, 10mm at 80mg/ml and 8mm at 50 mg/ml. Stenotrophomonas maltophilia had the lowest zone of inhibition of 10mm at 100mg/ml, 8mm at 80mg/l and 7mm at 50mg/ml. These zones of inhibitions show the antimicrobial potency of Jatropha tajorensis on antibiotic resistant bacteria. Compared with synthetic antibiotics or inorganic chemicals, this plant-derived extract is natural, less toxic, typically residue free with high rate of metabolism. The result of this study gives credence to claims of the medicinal and nutritional properties of Jatropha tanjorensis.
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Obafunmi, Tonidunni, Juliana Ocheme, and Bawa Gajere. "OLIGODYNAMIC EFFECT OF PRECIOUS METALS ON SKIN BACTERIA." FUDMA JOURNAL OF SCIENCES 4, no. 3 (2020): 601–8. http://dx.doi.org/10.33003/fjs-2020-0403-334.
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The oligodynamic hold of gold and silver is extant and extremely relevant as a proactive measure against infections. The antimicrobial effect of gold and silver jewelries was determined on skin bacteria by introducing them into culture plates already inoculated with bacterial isolates; Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa from the wrist, neck and ear regions of the skin. Samples were collected by swab sampling and isolates were identified by their cultural morphology and biochemical characterization. Results showed that both precious metals were able to inhibit all three bacteria with silver jewelries giving wider zones of inhibition measuring between 3 mm to 7 mm while gold jewelries gave zones of inhibition between 1 mm to 4 mm. The culture plates containing Pseudomonas aeruginosa recorded the highest zone of inhibition while the least was observed in the culture plates that contained Staphylococcus epidermidis. No zone of inhibition was observed with ordinary golden-coloured and silver-coloured jewelries which served as negative control. This study showed that gold and silver are oligodynamic with silver having a higher oligodynamic efficacy on the skin bacteria tested
 
 Key Words:
 Precious metals, Oligodynamic efficacy, Skin bacteria
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Anggraeni, Ratih, and Nola Monalisa Lingga. "TEST THE ANTIBACTERIAL ACTIVITY OF ETHANOL EXTRACT OF CRASSOCEPHALUM CREPIDIOIDES LEAF AGAINST STREPTOCOCCUS PYOGENES." JURNAL FARMASIMED (JFM) 4, no. 2 (2022): 121–26. http://dx.doi.org/10.35451/jfm.v4i2.1082.
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The ethanol extract of Crassocephalum crepidioides leaves was tested against Streptococcus pyogenes bacteria. Extracts from the leaves of Crassocephalum crepidioides are known to have the potential to suppress microbial growth because contain active compounds of alkaloids and flavonoids. The test was carried out by the diffusion method using disc paper that had been soaked in a solution of ethanol extract of Crassocephalum crepidioides leaves with a concentration of 20%, 30%, 40%, 50%. Amoxicillin as a positive control and DMSO as a negative control. It is known that the inhibition of bacterial growth at a concentration of 20% the inhibition zone is 11mm, the concentration of the inhibition zone of 30% is 11.8mm, the concentration of 40% of the inhibition zone is 12.2mm, the concentration of 50% is the inhibition zone is 12.6mm. The higher the concentration of the ethanol extract of Crassocephalum crepidioides leaves, the wider the area of inhibition against Streptococcus pyogenes bacteria.
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Suneet Kumar Sahni. "Evaluation of Antimicrobial Activity of Citrus Leaves against Gram Positive and Gram Negative Bacterial Strains." Proceeding International Conference on Science and Engineering 11, no. 1 (2023): 132–37. http://dx.doi.org/10.52783/cienceng.v11i1.107.
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In present study four bacterial strains, including Escherichia coli (Gram negative), Pseudomonas aeruginosa (Gram negative), Staphylococcus aureus (Gram positive), and Bacillus subtilis, were tested for antimicrobial activity of citrus leaf extracts in water, benzene, and methanol (Gram positive). According to the findings, Staphylococcus aureus (Gram positive) exhibited a 19 mm zone of inhibition in methanol extracts, followed by Bacillus subtilis (Gram positive) (16 mm). Staphylococcus aureus (Gram positive) showed zone of inhibition in benzene extracts was 17 mm, followed by Bacillus subtilis (Gram positive) (16 mm), and a similar pattern was seen in water extracts. Against Pseudomonas aeruginosa (a Gram negative bacteria), the zone of inhibition for gram negative bacteria was 12 mm in methanol extract, and a similar pattern was seen in benzene and water extracts.
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Sati, S. C., and Lokendra Singh. "Bioactivity of Root Endophytic Freshwater HyphomycetesAnguillospora longissima(Sacc. & Syd.) Ingold." Scientific World Journal 2014 (2014): 1–5. http://dx.doi.org/10.1155/2014/707368.
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Anguillospora longissima, isolated from root as endophytic freshwater hyphomycetes, was evaluated for its bioactivity (antibacterial potential) against five bacterial strains, namely, Gram-positive (Bacillus subtilisMTCC 121) and Gram-negative (Agrobacterium tumefaciensMTCC 609,Escherichia coliMTCC 40,Erwinia chrysanthemum,andXanthomonas pseudomonas). Antimicrobial activity was assessed by measuring the zone of inhibition with preliminary and secondary antimicrobial assays. The applied fungus was found significant for all tested bacterial strains as showen by their zone of inhibition. In preliminary antimicrobial assay, maximum zone of inhibition was recorded against Gram-negative human pathogenic bacterial strainEscherichia coli(23 mm) followed byErwinia chrysanthemi(22 mm),Agrobacterium tumefaciens(21 mm), andXanthomonas phaseoli(21 mm), while minimum zone of inhibition was observed againstBacillus subtilis(20 mm). In secondary antimicrobial assay, the maximum zone of inhibition was recorded againstErwinia chrysanthemi(11 mm) followed byAgrobacterium tumefaciens(10 mm),Xanthomonas phaseoli(10 mm), andBacillus subtilis(9 mm) and minimum inhibition was found againstEscherichia coli(8 mm).
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Asrianto, Asrianto, Asrori Asrori, Loly Sabrina Sitompul, Indra Taufik Sahli, and Risda Hartati. "Uji Aktivitas Ekstrak Etanol Biji Buah Merah (Pandanus conoideus Lamk.) terhadap Bakteri Escherichia coli dan Staphylococcus aureus." Bioscientist : Jurnal Ilmiah Biologi 9, no. 1 (2021): 1. http://dx.doi.org/10.33394/bjib.v9i1.3437.
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Infectious diseases can be caused by one of four different organisms, namely: bacteria, fungi, viruses, and parasites. This study aims to determine the ability and concentration of the best ethanol extract of red fruit seeds (Pandanus conoideus Lamk.) In inhibiting the growth of Escherichia coli and Staphylococcus aureus infectious bacteria. This research is an experimental study using a completely randomized design (CRD). The stages of work carried out consisted of: sample preparation, sample extraction, solution concentration preparation, and inhibition testing. The research work procedure began with the process of preparing a sample of red fruit seeds into powder. The maceration method was used for extraction using 96% ethanol with a ratio of 1: 3. The concentration of the extract was made by mixing the macerated extract with aquadest solvent. The concentration variations made were: 0.31%, 0.63%, 1.25%, and 2.50%. Inhibition testing against bacteria was carried out using the Kirby-Bauer method. The data obtained were analyzed using Analysis of Variance (ANOVA). The results showed that the ethanol extract of red fruit seeds had bioactive compounds that could inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria. The bioactive compounds consist of phenols, flavonoids, steroids, terpenoids, alkaloids, and tannins. The average diameter (mm) of the inhibition zone of Staphylococcus aureus bacteria was greater than the average diameter (mm) of the inhibition zone in Escherichia coli bacteria due to differences in the structure of their cell walls. The increase in the concentration of the solution is directly proportional to the diameter of the formed zone of inhibition. The higher the concentration given, the larger the diameter of the inhibition zone is seen. Duncan test analysis showed that 2.5% ethanol extract concentration was the best concentration in inhibiting bacterial growth.
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Andriansah, Agus, Fitria Lestari, and Destien Atmi Arisandy. "Antibactery test of dragon scale leaves (Drymoglossum piloselloides) patientaries on the hamble zone of Propionibacterium acnes." Symposium on Biology Education (Symbion) 3 (October 7, 2023): 221. http://dx.doi.org/10.26555/symbion.11708.
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Research on the benefits of the starch essence of dragon scale leaves (Drymoglossum piloselloides) against anti-bacterial P.acnes has not yet been conducted. The use of dragon's scales as an anti-bacterial on acne is intended to develop a new type of anti-bacterial that has minimal side effects against Propionibacterium acnes bacteria and to overcome resistance problems arising from the use of antibiotic drugs. This research uses laboratory experimental method with quantitative research type and the design used in the research is Post Test Only Control Group Design. The parameters measured were the antibacterial power of Drymoglossum piloselloides leaf starch juice against Propionibacterium acnes microbiologically by using the disc paper method, then the inhibition zone was measured using a caliper with an accuracy of 0.01 mm. The results of measuring the diameter of the inhibition zone of dragon scale leaf starch juice (Drymoglossum piloselloides) against Propionibacterium acnes inhibition zone, obtained the average diameter of the inhibition zone at a concentration of 40% of 1.26 mm with a weak category, at a concentration of 50% of 1.94 mm with a weak category, at a concentration of 60% of 2.8 mm with a weak category, at a concentration of 70% of 3.36 mm with a weak category, at a concentration of 80% of 3.94 mm with a weak category and in the positive control using Chloramphenicol get an average inhibition zone diameter of 8.36 mm with a moderate category
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Reetha, A. Karmel, and S. Mohan. "Inhibitory effect of bacterial antagonists on the growth of Macrophomina phaseolina (Tassi.) Goid. Causing charcoal rot of sunflower (Helianthus annus L.) invitro." Journal of Applied and Natural Science 7, no. 1 (2015): 489–92. http://dx.doi.org/10.31018/jans.v7i1.637.
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Charcoal rot caused by Macrophominaphaseolinais a major disease causing in sunflower plant. The pathogen invasion occurs from the seedling to maturity stage. To overcome this problem in vitro, sensitivity of M. phaseolina was determined through inhibition zone technique to various isolates of antagonistic bacteria like seven isolates of Pseudomonas fluorescens (EPf2, EDPf3, APf4, CPf5, MPf6, KPf7 and Pf1)and seven isolates Bacillus subtilis (EBs1, EDBs2, ABs3, CBs4, MBs5, KBs6 and Bs10) amended into PDA medium. The results showed that the entire bacterial antagonist were effective against the fungus M. phaseolina and exhibited appreciable amount of inhibition. Among these bacterial antagonist significantly compared to the control P.fluorescens (Pf1) proved to be the most effective (71.49 %) with an inhibition zone of 5.00 mm reducing the colony growth of M. Phaseolina followed B. subtilis (65.92 %)inhibition zone of 17.80 mm respectively over control. However, from these studies it is concluded that an isolate of various antagonist can vary in its sclerotia producing ability on root.
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Mohamed, Nather Ibraheem, Salma M. Hassan, and Khaleed J. Khalil. "Preparation, Characterization, and Antimicrobial Activity of Polyaniline and Fe2O3/Polyaniline Composite Nanoparticle." Iraqi Journal of Physics (IJP) 20, no. 1 (2022): 48–56. http://dx.doi.org/10.30723/ijp.v20i1.725.
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An oxidative polymerization approach was used to create polyaniline (PANI) and Fe2O3 /PANI nanoparticle combination. Various characterization approaches were used to investigate the structural, morphological, and Fe2O3 /PANI nanoparticle structures. The findings support the synthesis of polycrystalline nanoparticle PANI and Fe2O3 /PANI spherical nanoparticle composites. Gram-positive bacteria are tested for antibacterial activity. Various quantities of Nanoparticles of PANI and Fe2O3 /PANI nanoparticle composites were used to test Staph-aureus and gram-negative bacteria, E-coli, and candida species. PANI has antibacterial properties against all microorganisms tested. Fe2O3 /PANI nanoparticle composites, on the other hand, have higher antibacterial activity, as evidenced by the zone of inhibition. Bacterial inhibition zones are in S. aureus (positive), and E. coli are in good functioning order. With increasing concentrations of Fe2O3 /PANI nanoparticle composites, the inhibition zones of all bacteria are larger. Finally, the antimicrobial activity of Fe2O3 /PANI nanoparticle composite is characterized using a simplified mechanism based on electrostatic attraction. In this paper, a conductive polymer doped with iron nanoparticles was fabricated for the aim of testing it in the field of bacterial resistance.