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Ginting, Elvy Like, Liviani Rangian, Letha L. Wantania, and Stenly Wullur. "Isolation of Symbiotic Bacteria with Red Algae from Tongkaina Waters, North Sulawesi." JURNAL ILMIAH PLATAX 7, no. 2 (July 7, 2019): 395. http://dx.doi.org/10.35800/jip.7.2.2019.23728.
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This study aims to isolate symbiotic bacteria with red algae from the waters of Tongkaina, North Sulawesi. The red algae taken were red algae that are similar to the genus Portieria and Gracilaria. Bacteria were cultured and isolated using Nutrient Agar (NA) + sea water. Before bacteria from red algae samples were cultured, each sample of red algae was crushed, homogenized and diluted. The results of this study, 5 bacterial isolates were successfully isolated from red algae similar to Portieria sp. and 5 bacterial isolates from red algae similar to Gracilaria sp. Each bacterial isolate has different morphological characteristics such as shape, colour, elevation, and edges. Of the 10 bacterial isolates, 3 bacterial cells are gram-positive cocci (round), and 7 of them are gram-negative bacilli (rod).Keywords: Red algae, bacteria, isolation, symbionts ABSTRAKTujuan dari penelitian ini adalah untuk mengisolasi bakteri simbion dengan alga merah dari perairan Tongkaina, Sulawesi Utara. Alga merah yang diambil adalah alga merah yang mirip dengan genus Portieria dan Gracilaria. Bakteri ditumbuhkan dan diisolasi menggunakan media Nutrien Agar (NA) + air laut. Sebelum bakteri dari sampel alga merah ditumbuhkan, masing-masing sampel alga merah digerus, dihomogeniasi dan diencerkan. Hasil penelitian ini, 5 isolat bakteri berhasil diisolasi dari alga merah mirip Portieria sp. dan 5 isolat bakteri dari alga merah mirip Gracilaria sp. Masing-masing isolat bakteri memiliki karakteristik morfologi yang berbeda seperti bentuk, warna, elevasi, dan tepian. Dari 10 isolat bakteri tersebut, 3 sel bakteri bersifat gram positif dengan bentuk bulat, dan 7 diantaranya bersifat gram negatif dengan bentuk batang.Kata kunci: Alga merah, bakteri, isolasi, simbion
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Mawati, Sefi Desfeni, Esti Harpen, and Hilma Putri Fidyandini. "SKRINING BAKTERI TERMOFILIK POTENSIAL AMILOLITIK DARI SUMBER AIR PANAS WAY BELERANG KALIANDA LAMPUNG SELATAN." Journal of Aquatropica Asia 6, no. 1 (July 6, 2021): 1–7. http://dx.doi.org/10.33019/aquatropica.v6i1.2458.
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Thermophilic bacteria that produced amylase and protease have been isolated from Way Belerang hot spring, Kalianda, South Lampung. This research aims to screen and identify thermophilic bacteria that have the potential to produce thermostable amylase and protease enzymes.The research procedures included sampling, isolation of enzyme-producing thermophilic bacteria, a series of phenotypic and biochemical tests, and molecular identification by 16s rRNA. This study used 2 treatments, namely incubation temperature 37 and 50 ºC with 3 repetitions. The results showed that the optimum temperature for growth of thermophilic bacterial isolates and thermophilic bacterial isolates producing amylase enzymes was 50ºC. The bacteria isolate that had the best amylolytic enzyme activity was Isolate A.WB.50.1 with a diameter of the inhibitory zone was 15.44 mm. Isolate A.WB.50.1 has been identified by the species Pseudomonas stutzeri.
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IRAWATI, WAHYU, SEMUEL RIAK, NIDA SOPIAH, and SUSI SULISTIA. "Heavy metal tolerance in indigenous bacteria isolated from the industrial sewage in Kemisan River, Tangerang, Banten, Indonesia." Biodiversitas Journal of Biological Diversity 18, no. 4 (December 7, 2017): 1481–86. http://dx.doi.org/10.13057/biodiv/d180425.
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Irawati W, Riak S, Sopiah N, Sulistia S. 2017. Heavy metal tolerance in indigenous bacteria isolated from the industrial sewage in Kemisan River, Tangerang, Banten, Indonesia. Biodiversitas 18: 1481-1486. The bacterial study is a part of human calling in preserving the earth. Many indigenous bacteria isolated from heavy metal contaminated sites had resistance to heavy metal toxicity and could be used for heavy metal removal. The aims of this study were to isolate heavy metal-tolerant indigenous bacteria from the industrial sewage of Kemisan River in Tangerang, Banten, Indonesia. The potency of bacterial isolates to remove heavy metals was also determined. The heavy-metal tolerance was determined by measuring the minimum inhibitory concentration. The potency of bacterial isolate for removing heavy metals from the medium was determined by an atomic absorption spectrophotometer. The results showed that there were eight heavy metal-resistant bacteria isolated from Kemisan River with minimum inhibitory concentration ranging from 7 mM to 11 mM. Isolate PbSI1 was the highest lead tolerant bacteria, and also tolerant to copper and zinc. The isolate was able to remove 91.25% lead, 73.38% zinc, and 98.57% copper from medium supplemented with the mixture of these heavy metals. The addition of 9 mM of lead in the medium affected the morphological appearance of isolate colonies i.e PbSI1 and PbSI3 to become darker which might occur due to the survival mechanism of bacteria by absorbing the lead inside the cells. The finding of this study indicated that isolate PbSI1 was a promising bacterium, which could be further developed for heavy metal removal.
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Melizah, Ardesy, Syarif Husin, and Syifa Alkaf. "Identification of Lactic Acid Bacteria Isolate From Fermentation Food Bekasam." Bioscientia Medicina : Journal of Biomedicine and Translational Research 2, no. 1 (January 13, 2018): 16–23. http://dx.doi.org/10.32539/bsm.v2i1.34.
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Bekasam is a local food from South Sumatera Indonesia, which is obtained through fermentation of fish. Previous study has shown that fermented food contained Lactic Acid Bacteria (LAB) bacteria, such as Lactobacillus plantarum, Lactobacillus sp, Pediococus sp and Weissella sp. The study was conducted to identify isolate LAB from bekasam. Bekasam contained fish, rice, and salt with a certain ratio. Further isolation of isolated LAB isolate, then performed PCR for bacterial analysis. Isolate 1 showed alleged bacteria Staphylococcus sp, non pathogen, while isolate 2 leads to Lactobacillus sp. The PCR results show the suitability of the bands formed between the Lactobacillus standard and the isolates.
Keywords: bacteria, lactobacillus, acid
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Fajri, Muhammad Danial, Subehan Lallo, and Sartini Sartini. "PRELIMINARY STUDIES ON ISOLATION AND SCREENING OF ANTIBIOTIC PRODUCING SYMBIOTIC BACTERIA FROM STARFISH (Protoreaster nodosus) COLLECTED FROM COASTAL AREA TAKALAR REGENCY, INDONESIA." Journal of Experimental Biology and Agricultural Sciences 9, no. 2 (April 25, 2021): 183–88. http://dx.doi.org/10.18006/2021.9(2).183.188.
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Marine organisms are well known for the availability of bioactive compounds which have various biological activities including antibacterial activity. Likewise, their symbiotic bacteria can also produce compounds that have similar activities. The purpose of this study was to isolate and screen the symbiotic bacteria from starfish (Protoreaster nodosus) collected from coastal area Takalar Regency, South Sulawesi, Indonesia. Isolation was carried out by the pour plate method using nutrient agar medium dissolved in sterile seawater. The isolated symbiotic bacteria were purified by using the quadrant method. The pure isolate was culture through submerged fermentation using nutrient broth media enriched with 1% yeast extract and sterile seawater for 7 days. The selected symbiont bacterial isolates were tested for their antibacterial activity against gram-positive and gram-negative bacteria using disc diffusion assays. The results of fermentation were separated from the biomasses and tested for antibacterial activity against Staphylococcus aureus (S. aureus, ATCC 25923), Bacillus subtilis (B. subtilis, ATCC 6633), Salmonella typhi (S. typhi, NCTC 786), and Escherichia coli (E. coli, ATCC 25923). The results of study revealed that four symbiotic bacteria (SB 1T, SB 2T, SB 3T, and SB 4T) were successfully isolated. All the SB isolates have good antibacterial activity against all tested bacterial strains with an average diameter of inhibition zone larger than 11 mm. Among all isolates, isolate SB 4T showed a remarkable size of zones growth inhibition (> 15 mm) against all tested bacterial strains. Thus, the symbiotic bacteria isolated from P. nodosus in this study have a promising broad-spectrum antibacterial activity.
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Mulyati, Mulyati, Suryati Suryati, and Irfani Baga. "The Effectiveness of Candidate Probiotic Bacteria to Control Vibriosis Disease." Aquatic Science and Technology 5, no. 2 (July 24, 2017): 1. http://dx.doi.org/10.5296/ast.v5i2.11594.
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The study aims to isolate, characterize, and examine probiotic bacteria's inhibitory ability against Vibrio harveyi bacteria, both in-vitro and in vivo. Methods used in the study consist of 1) An Isolation of Candidate Probiotic Bacteria, 2) An Antagonistic Test of Candidate Probiotic Bacteria in vitro, 3) An Identification of Bacteria, 4) A Pathogenicity Test of Candidate Probiotic Bacteria, 5) An Antagonistic Test of Candidate Probiotic Bacteria against V. harveyi in vivo. According to the isolation of candidate probiotic bacteria, there are 18 isolated candidate probiotic. After being tested for its inhibitory ability in vitro, there are 8 isolates with zone of inhibition as follows: isolate MM 7 from intestine (22 mm), isolate MM 6 from intestine (12 mm), isolate MM 10 from sea water (10 mm), isolate MM 5 from intestine (9 mm), isolate MM 4 from intestine (8 mm), isolate MM 3 from intestine (7 mm), isolate MM 2.2 from intestine (7 mm), isolate MM 2.1 from intestine (7 mm). Eight genera of the candidate probiotic bacteria is derived from Portunid crab, they are Staphylococcus, Streptococcus, bacillus, vibrio, Alcaligenes, Lactobacillus, micrococcus. Before proceeding the V. harveyi bacterial challenge test in vivo, three potential isolates consisting of MM6, MM7 and MM10 as the probiotic bacteria are pathogenicity-tested against V. harveyi. The survival rate of Portunid crab on pathogenicity test using MM6, MM7 and MM10 generates 91.11-100%, while the control generates 100% survival rate. Variance analysis result through post-hoc Tukey's Honest Significant Difference (HSD) test at 95% confidence interval indicates that isolate MM7 and MM10 are significantly able to increase hatchling Portunid crab's survival rate.
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Rotua Silitonga, Lamtiur, Nursyirwani Nursyirwani, and Irwan Effendi. "ISOLATION, IDENTIFICATION AND SENSITIVITY OF AMILOLITIC BACTERIA FROM MANGROVE ECOSYSTEM SEDIMENT IN PURNAMA MARINE STATION DUMAI ON THE PATHOGENIC BACTERIA." Asian Journal of Aquatic Sciences 2, no. 3 (January 24, 2020): 257–66. http://dx.doi.org/10.31258/ajoas.2.3.257-266.
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Litter from the weathering of dead mangrove stems and leaves contains a lot of starch which has potential to be degraded by amylolytic bacteria into simple compounds with the help of the amylase enzyme. Amylolytic bacteria are bacteria that hydrolyze starch into simpler compounds namely glucose with the help of the amylase enzyme. This study aims to 1) isolate, identify and test sensitivity of amylolytic bacterial isolates found at the Purnama Dumai Marine Station, 2) the ability of amylolytic bacterial isolates to inhibit the growth of pathogenic bacteria (Escherichia coli, Pseudomonas aeruginosa and Vibrio alginolyticus) and 3) to determine the of amylolytic bacterial species by 16S rRNA sequence analysis. The results showed 10 bacterial isolates (TR 2, TR 6, TR 7, TR 9, TR 11, TR 13, TR 15, TR 16, TR 18 and TR 20) were able to inhibit the growth of pathogenic bacteria (E.coli, P.aeruginosa and V.alginolyticus). The sensitivity test of isolate TR 20 against E.coli was categorized into weak with inhibition zone diameter of 4.65 mm. Sensitivity of isolate TR 6 against P.aeruginosa was categorized into medium with inhibition zone diameter of 5.22 mm. Then sensitivity of isolate TR 11 against V.algynolyticus was categorized into medium with inhibition zone diameter of 5.55 mm. DNA analysis using 16S rRNA method and BLAST analysis showed similarity of each isolate. Isolate TR 6 was similar to Bacillus paramycoides strain MCCC 1A04098, isolate TR 11 was in a group of Enterobacter cloacae strain ATCC 13047 and TR 20 was in a group of Vibrio harveyi strains of NBRC 15634.
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Msango Soko, K. R., R. C. Bhattacharya, B. Ramakrishnan, K. Sharma, and S. Subramanian. "Functional characterization of bacteria isolated from different gut compartments of white grub, Anamola dimidiata, larvae." Journal of Environmental Biology 41, no. 6 (November 15, 2020): 1526–35. http://dx.doi.org/10.22438/jeb/41/6/mrn-1420.
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Aim: The aim of the present study was to isolate and characterize cellulolytic, lipolytic and nitrate reductase activities in the bacteria isolated from the gut of white grub, Anamola dimidiata larvae Methodology: Field collected third instar scarab larvae were dissected under aseptic conditions and inoculated on different bacteriological media to isolate gut bacteria. Identification of these isolates was carried out by amplifying and sequencing the 16S rRNA gene and comparing with their closest relatives in GenBank. Cellulolytic, lipolytic and nitrate reductase activities were assayed using Carbonmethyl cellulose (CMC), Rhodamine B and nitrate broth media. Results: The majority of culturable bacteria in the gut of A. dimidiata belonged to two phyla: Firmicutes (62.5%) and Proteobacteria (37.5%). Forty aerobic and eleven anaerobic bacterial strains were isolated and tested for cellulolytic, lipolytic and nitrate reductase activity, and twenty seven and thirty one cellulolytic and lipolytic gut bacteria were identified, respectively, with 19 isolates exhibiting both activities whereas ten facultative anaerobic bacteria isolates were positive for nitrate reductase activity. Interpretation: These bacterial isolates may be good sources for profiling novel isolates and enzymes for industrial use besides identifying new solutions for pest control.
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Sari, Indah Juwita, and I. Nyoman Pugeg Aryantha. "Screening and Identification of Mushrooms Growth Promoting Bacteria on Straw Mushrooms (Volvariella volvacea)." Journal of Tropical Biodiversity and Biotechnology 6, no. 1 (January 18, 2021): 60618. http://dx.doi.org/10.22146/jtbb.60618.
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This research aimed to identify the indigenous Mushroom Growth Promoting Bacteria (MGPB) bacteria that can increase the growth of Volvariella volvacea. The research began by isolating indigenous MGPB from planting media of straw mushrooms in Karawang, Indonesia. The screening was performed to select bacterial isolates that can promote the highest growth of mushrooms by dual culture method on PDA media. There were 10 of the 58 highest bacterial isolates that have a positive effect on the vegetative growth of mushrooms. The 23K bacterial isolate was the most significant increase in mycelium growth compared to other isolates and bacteria-induced controls. A bacterial isolate 25K by gene analysis was identified by 16S rRNA (518F primer (5’- CCA-GCA-GCC-GCG-GTA-ATA-CG -3’) and 800R primer (5’- TAC-CAG-GGT-ATC-TAA-TCC -3’). The result from gene analysis shows that there are ~1550 base pairs products. BLAST analysis and phylogenetic tree adjustment results show that the closest diversity of this bacterial isolate 25K is Bacillus thuringiensis serovar konkukian str. 92-27 (equality value = 99%).
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Irma, Ade, Anja Meryandini, and Bedah Rupaedah. "Biofungicide Producing Bacteria: an In Vitro Inhibitor of Ganoderma boninense." HAYATI Journal of Biosciences 25, no. 4 (December 4, 2018): 151. http://dx.doi.org/10.4308/hjb.25.4.151.
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Oil palm is widely known as one of vegetable oil sources and the main comodity in Indonesian agriculture because of the benefits in non-food and food industries. Ganoderma boninense attack results in considerable losses to agriculture. Chemical control creates a harmful effect on health and the environment. Biocontrol is required to take over the function of chemical control. This study aimed to select bacteria that produce bioactive compounds as biofungicide against G. boninense pathogenic fungi and identify bacteria producing biofungicide using molecular method. The stages of bacterial isolate selection were performed through the selected hemolysis and isolate tests in the antagonistic test. Bacteria were extracted using ethyl acetate and their extract activity were tested. Analysis of bioactive compounds was conducted using thin layer chromatography (TLC) and the identification was based on 16S rRNA gene. The result of bacterial pathogenic test was obtained from two selected bacterial isolates namely 11B LB and 11B MD. Both bacterial isolates showed antagonistic effects by forming an inhibitory zone against G. boninense growth with percentage of inhibitor of 81 and 75%. Activity test of bacterial extract showed that crude extract of bacterial isolate 11B MD had the highest inhibitor activity that is 88.34%. TLC analysis proved that the active extract of bacteria containing metabolite compounds had Rf value of 0.1, 0.28, and 0.38. Isolate bacteria 11B MD was identified as Pseudomonas aeruginosa.
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Kurniawan, Ardiansyah, Suci Puspita Sari, Euis Asriani, Andi Kurniawan, Abu Bakar Sambah, and Asep Awaludin Prihanto. "Molecular Identification of Cellulolytic Bacteria From Mangrove Sediment at Tin Minning Region In West Bangka." International Journal of Applied Biology 3, no. 1 (June 29, 2019): 7. http://dx.doi.org/10.20956/ijab.v3i1.5848.
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Cellulose as an abundant source of glucose in Indonesia requires acceleration of decomposition utilizing cellulolytic bacteria. Cellulolytic bacteria can be obtained from the isolation of mangrove organic matter, such as sediments. Muntok Sub-district is one of the regions with the most tin mining in West Bangka Regency also has mangroves in the coastal area. Exploration of cellulolytic bacteria in mangroves with different environmental characteristics encourages researchers to find new bacterial strains that produce cellulase enzymes with new properties. Thirteen isolates were successfully isolated from three locations. Tembelok mangrove sediments produced Seven bacterial isolates, Peltim Mangrove samples produced three isolates and from Sukal Mangrove three isolates were obtained. Seven isolates showed clear zones in the Lugol test and three isolates including were gram-positive bacteria. Molecular test with 16S rRNA analysis showed TBL1 isolate has 85% similar identity of Vibrio parahaemolyticus strain HY3 and TBL2 isolate has 98% similar identity of Bacillus amyloliquefaciens strain HS8. Bacillus amyloliquefaciens potential to further study as cellulose degrading bacteria for feed ingredients.
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Masrukhin, Masrukhin, Ade Lia Putri, Tri Ratna Sulistiyani, Muhammad Ilyas, Ismu Purwaningsih, Iwan Saskiawan, and Muhammad Yusrun Niam. "Antifungal Activity of Bacterial Isolates from Straw Mushroom Cultivation Medium against Phytopathogenic Fungi." Journal of Tropical Biodiversity and Biotechnology 6, no. 1 (January 20, 2021): 59235. http://dx.doi.org/10.22146/jtbb.59235.
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Several bacteria were isolated from straw mushroom (Volvariella volvacea) cultivation medium. There are three potential isolates previously characterized and has growth inhibition effect against V. volvacea. This screening result lead to the further study about the inhibition activity against phytopathogenic fungi. The aim of this research is to investigate the antifungal activity of three bacterial isolates against three phytopathogenic fungi and identification of the bacteria. The method used in this study are antifungal assay using co-culture method and disk difussion assay using the filtrate of each bacteria. The profile of antifungal compound was identified using ethyl acetate extract followed by evaporation and gas chromatography (GC-MS) analysis. Identification of each isolates was performed using 16S rDNA amplification and sequencing. Three phytopathogenic fungi i.e Cercospora lactucae (InaCC F168), Colletotrichum gloeosporides (InaCC F304) and Fusarium oxysporum f.sp. cubense (F817) were co-cultured with bacterial isolates C2.2, C3.8, and D3.3. The C3.8 isolate has highest average inhibition activity either using isolate and filtrate. The result relatively consistent against three phytopathogenic fungi. The metabolite profile of C3.8 isolate showed the Bis(2-ethylhexyl) phthalate as the main compound with 97% similarity. Bis(2-ethylhexyl) phthalate has potential effect as antibacterial and antifungal compound. According to EzBioCloud and GeneBank databases, the C2.2 isolate identified as Bacillus tequilensis, C3.8 as Bacillus siamensis and D3.3 as Bacillus subtilis subsp. Subtilis. This study also shows the potential of Bacillus siamensis C3.8 as biocontrol against phytopathogenic fungi.
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Hernández-Huerta, Jared, Patricia Tamez-Guerra, Ricardo Gomez-Flores, Ma Carmen E. Delgado-Gardea, Margarita S. García-Madrid, Loreto Robles-Hernández, and Rocio Infante-Ramirez. "Prevalence of Xanthomonas euvesicatoria (formally X. perforans) associated with bacterial spot severity in Capsicum annuum crops in South Central Chihuahua, Mexico." PeerJ 9 (February 15, 2021): e10913. http://dx.doi.org/10.7717/peerj.10913.
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Background Xanthomonas spp. causes bacterial spot disease, which reduces quality and yield of pepper crops in Mexico. Identification of phytopathogen species is necessary to implement more effective control strategies. Objective The aim of this study was to isolate and identify infecting Xanthomonas species in South Central Chihuahua pepper-producing areas. Methods Diseased plants were collected from 30 cultivation lots and bacteria were isolated from damaged tissues. Potential causative agents were isolated, identified, and characterized by biochemical and molecular analysis. Pathogenicity tests from each isolate were then performed on 30-d-old pepper seedlings, exposing five leaves to 10 µL of 1 × 108 CFU/mL bacterial suspensions of each isolate, using sterile distilled water as a control. Disease severity was determined after 10 d by calculating leaf damage percentage. Furthermore, we evaluated the susceptibility of the highest bacterial spot severity-causing isolates (13 isolates) to copper sulphate (CuS), copper gluconate (CuG), copper oxychloride + oxytetracycline hydrochloride (Cu + Ox), gentamicin + oxytetracycline hydrochloride (Gen + Ox), and gentamicin sulphate (GenS). Copper-resistance genes (copLAB) were detected by PCR analysis among isolates. Results Thirty-seven foliage isolates were identified as Xanthomonas euvesicatoria (14%), which were associated with bacterial spot disease in jalapeño pepper. Tested Xanthomonas isolates were resistant to Cu-based compounds, but susceptible to Cu + Ox. All isolates were susceptible to Gen + Ox and GenS. CopLAB genes were detected in all but one strain. Conclusions X. euvesicatoria (formally X. perforans) may be considered as an emerging pathogen of bacterial spot pepper in Mexico. Among disease management strategies, alternatives to copper should be taken into consideration.
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Sepriana, Citra, and Eti Sumiati. "Identifikasi Dan Uji Daya Hambat Isolat Bakteri Endofit Bunga Tanaman Cengkeh (Syzygium aromaticum L.) Terhadap Bakteri Patogen." Jurnal Penelitian Pendidikan IPA 6, no. 1 (January 29, 2020): 101. http://dx.doi.org/10.29303/jppipa.v6i1.340.
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This research was conducted to find out the capabilities of endophytic bacteria isolated from flowers of the clove plants in inhibiting the growth of bacteria Streptococcus mutans, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli and to identify endophytic bacteria that potensial to produce an antibacterial. Stages of this research include the isolation of endophytic bacteria from flowers of the clove plants, antibacterial test, and molecular identification based on 16S rRNA. Isolates of endophytic bacterial of clove plants flower produce 5 isolates, 4 isolate inhibited the bacteria S. aureus. Based on 16S rRNA molecular identification, endophytic bacterial isolates of clove plants flower which have inhibitory closely related to Bacillus amyloliquefasiens, Staphylococcus epidermidis 1034 MPA and Bacillus cereus JL.
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Ryan, N. Aileen, and Peter Jones. "The ability of rhizosphere bacteria isolated from nematode host and non-host plants to influence the hatch in vitro of the two potato cyst nematode species, Globodera rostochiensis and G. pallida." Nematology 6, no. 3 (2004): 375–87. http://dx.doi.org/10.1163/1568541042360528.
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AbstractSeventy bacteria, isolated from the rhizosphere of the potato cyst nematode (PCN) host plant, potato, were cultured in the presence and absence of potato root leachate (PRL) and the resultant culture filtrates were analysed for their ability to affect the hatch in vitro of the two PCN species. Of the isolates tested, nine had a significant effect on PCN hatch. Six affected Globodera pallida hatch and three affected G. rostochiensis hatch. Five of the isolates significantly increased hatch only when cultured in the presence of PRL. Three of the isolates decreased PCN hatch significantly in PRL. Only one isolate increased hatch significantly in the absence of PRL. No isolate affected the hatch of both species. Six of the nine isolates that significantly affected PCN hatch had been pre-selected by culturing on PRL. Bacterial isolates from PCN non-hosts (14 from wheat, 17 from sugar beet) were also tested for hatching activity. The principal effect of the hatch-active isolates from the PCN non-host plants was to increase PCN hatch in the presence of PRL. In contrast to the host bacteria results, the isolates from non-host plants affected only G. rostochiensis hatch (three wheat isolates and four sugar beet isolates significantly increased G. rostochiensis hatch); no such isolate affected G. pallida hatch significantly in the presence of PRL. Ten isolates (32%) from non-host plants had the ability to increase significantly the hatch of PCN in the absence of PRL (eight of these affected G. rostochiensis hatch and four affected G. pallida hatch), compared to only one bacterial isolate (1%) from a host plant. The majority of the isolates from non-hosts produced PCN species-specific effects, as with the bacteria isolated from potatoes, although two wheat isolates increased the hatch of both species significantly in the absence of PRL. Of 20 hatch-active bacterial isolates (from all three plants) identified, 70% were Bacillus spp. Other genera identified were Arthrobacter , Acinetobacter and Staphylococcus .
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Singh, Mahendra. "Isolation and characterization of insoluble inorganic phosphate solubilizer rice rhizosphere strain Enterobacter cloacae BAU3." Journal of Applied and Natural Science 10, no. 4 (December 1, 2018): 1204–9. http://dx.doi.org/10.31018/jans.v10i4.1929.
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The objective of the present study was to isolate and characterize most efficient phosphate solubilizing bacteria (PSB) from rice rhizosphere. The study was carried out during the Kharif season’2018 at Department of Soil Science and Agricultural Chemistry, Bihar Agricultural University, Sabour, Bhagalpur, Bihar. The availability of phosphorous to plants for uptake and utilization is limited in soil due to fixation in the form of Fe-P, Al-P and Ca-P. The use of phosphate solubilizing bacteria can prove to be helpful measure to supply phosphorous to the crops to increase the productivity. In the present investigation, a total of 10 isolates were obtained from rice rhizosphere soil samples. All ten isolated isolates were shown phosphorus solubilization. Out of ten isolates BAU3 was found to be most potent phosphate solubilizers showing clear halo zone around its colony. The isolate BAU3 showed 20.00 mm phosphate solubilizing halo zone around its colony. The solubilization index (SI) of the isolate BAU3 was also calculated at the end of the incubation period and observed phosphate solubilization index (SI) of 3.22. The isolate BAU3 showed maximum insoluble phosphate solubilization of 450.24 ?g ml-1 and isolates BAU3 was selected for subsequent studies. The bacterial isolates BAU3 was gram negative, non-spore forming rods shaped. On the basis of the 16SrDNA sequencing, isolate BAU3 was identified as Enterobacter cloacae strain BAU3 (Genebank Accession No. MK033472). The isolated strain of bacterial has potential to solubilize insoluble phosphorus and it can be utilized for preparation of microbial inoculants or biofertilizers.
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Wulandari, Erna, Endah Retnaningrum, and Langkah Sembiring. "Identification Bacterial Contaminant in Semiarundinaria fastuosa Tissue Culture." Proceeding International Conference on Science and Engineering 3 (April 30, 2020): 39–42. http://dx.doi.org/10.14421/icse.v3.465.
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Bamboo is one of the plants which propagated by tissue culture technique however, the emergence of microbial contaminant caused decrease of bamboo production. A type of microbe that caused the contamination on Semiarundinaria fastuosa tissue culture is bacteria. This study aimed to isolate bacterial contaminant and understanding its biodiversity. Bacterial isolation and phenotypic characterization were done by observing morphology, physiological test, biochemical test, identification and numeric phenetic analysis. Twelve bacterial contaminants was isolated and based on profile matching with Bergey’s Manual of Determinative Bacteriology, six isolate was a member of genus Bacillus, one isolate Enterococcus, two isolate Xenorhabdus, one isolate Morococcus, one isolate Corynebacterium, and one isolate belongs to genus Sarcina. A dendrogram created using Simple Matching coefficient (SSM) parameter and average linkage algorithm shown that on the 71% similarity index, all 12 OTU was grouped in one cluster. However, a dendrogram produced by Jaccard’s coefficient (SJ) parameter and average linkage algorithm on 70% similarity index divided 12 OTU on to 12 clusters.
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SHEFFIELD, CYNTHIA, KATE ANDREWS, ROGER HARVEY, TAWNI CRIPPEN, and DAVID NISBET. "Dereplication by Automated Ribotyping of a Competitive Exclusion Culture Bacterial Isolate Library." Journal of Food Protection 69, no. 1 (January 1, 2006): 228–32. http://dx.doi.org/10.4315/0362-028x-69.1.228.
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Concerns over the development of antibiotic-resistant bacteria within the food animal industry have intensified the search for natural approaches to the prevention and treatment of bacterial diseases. Competitive exclusion cultures are the foundation of a disease-management strategy based on the use of benign bacterial strains to prevent the establishment of pathogenic bacteria within a specific host. Differentiation of phenotypically ambiguous isolates is a critical step in establishing a manageable library of bacteria for use in the development of defined competitive exclusion cultures. We used automated ribotyping techniques to dereplicate a large collection of phenotypically ambiguous isolates from a continuous-flow competitive exclusion culture. A total of 157 isolates were screened following an EcoRI restriction enzyme digestion. The 157 isolates were resolved into 23 ribogroups, which represents an 85% reduction in the number of isolates in the bacterial isolate library. Seventy-six percent of the isolates fit into one of five ribogroups. This work demonstrated that automated ribotyping is an effective and efficient tool for dereplication of diverse bacterial isolate libraries.
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Prasad V, H., G. Kumar, Kartik L, and B. Rao KV. "Screening of Extracellular Keratinase Producing Bacteria from Feather Processing Areas in Vellore, Tamil Nadu, India." Journal of Scientific Research 2, no. 3 (August 24, 2010): 559. http://dx.doi.org/10.3329/jsr.v2i3.4567.
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The aim of the current study was to isolate keratinolytic bacteria from the soil samples collected from different feather processing areas in Vellore, TN, India. The isolation was performed by serial dilution and plating method. Total eight bacteria were isolated from the collected soil samples. All isolates were screened for keratinolytic activity by Casein agar plate method, among eight bacterial isolates only one (H5) isolate showed the keratinolytic activity in Casein agar medium. H5 isolate (potential strain) was identified as Bacillus sp. by microscopic and biochemical experiments. The best enzyme activity was observed at pH 7 and temperature 30ºC. Keywords: Keratinolytic; Spread plate method; Bacillus sp.; Bacteria. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i3.4567 J. Sci. Res. 2 (3), 559-565 (2010)
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Ambarsari, Hanies, Luftiara Asriyani, and Aflakhur Ridlo. "Isolasi dan Produktivitas Bakteri Ureolitik dari Sedimen Muara Sungai Citarum." Jurnal Teknologi Lingkungan 21, no. 2 (July 30, 2020): 147–56. http://dx.doi.org/10.29122/jtl.v21i2.3970.
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ABSTRACTThis research carried out isolation of ureolytic bacteria taken from soil samples from the mangrove area of Muara Gembong, Citarum River. The aim was to characterize and to test the urease production activity of the ureolytic bacteria. The study was conducted at the BPPT Environmental Laboratory in Building 820 Geostech, Puspiptek Setu, South Tangerang City, Banten Province, from April until September 2019. The results showed that there were ureolytic bacterial isolates from soil samples taken. The productivity of ureolytic bacterial isolates was indicated by the increasing ammonia concentration and the increase of bacterial cell concentration supported by the pH and temperature factors per volume unit of 10 ml. The increase in ammonia concentration of the six isolates’ test media showed significant differences. The highest increase in ammonia concentration was 15.19 ppm produced by isolate K5A and the second highest was 14.10 ppm by isolate K7. Another productivity testing was performed by determining if there was an increase in the concentration of bacterial cells. Based on the analysis of variance, each isolates significantly affected the increase in bacterial cell concentration. The highest increase in bacterial cell concentration by 250.45×106 CFU/ml occurred in isolates K5A, while isolate K7 indicated the second-highest increase in cell concentration by 233.31×106 CFU/ml.Keywords: ureolytic bacteria, productivity, mangrove sediment, Citarum River EstuaryABSTRAK Penelitian ini melakukan isolasi bakteri ureolitik yang diambil dari sampel tanah dari daerah mangrove dari di Muara Gembong, Sungai Citarum. Tujuannya adalah untuk melakukan karakterisasi dan menguji aktivitas produksi urease terhadap isolat bakteri ureolitik yang ditemukan. Penelitian dilakukan di Laboratorium Lingkungan BPPT di Gedung 820 Geostech, Puspiptek Setu, Kota Tangerang Selatan, Provinsi Banten mulai bulan April sampai dengan September 2019. Enam isolat bakteri dapat diperoleh dari sampel tanah yang diambil. Produktivitas isolat bakteri ureolitik pada penelitian ini dapat ditandai dengan kenaikan konsentrasi amonia kenaikan konsentrasi sel bakteri yang didukung dengan faktor pH dan suhu per satuan volume 10 ml. Kenaikan konsentrasi amonia dari keenam media uji isolat menunjukkan perbedaan yang nyata. Produksi konsentrasi amonia tertinggi sebesar 15,19 ppm dihasilkan oleh isolat K5A dan tertinggi kedua sebesar 14,10 ppm oleh isolat K7. Untuk uji produktivitas juga dilakukan dengan melihat adanya kenaikan konsentrasi sel bakteri. Berdasarkan analisis variansi, masing-masing isolat berpengaruh nyata terhadap kenaikan konsentrasi sel bakteri. Kenaikan konsentrasi sel bakteri tertinggi sebesar 250,45×106 CFU/ml terjadi pada isolat bakteri K5A sedangkan isolat K7 menunjukkan kenaikan konsentrasi sel tertinggi kedua sebesar 233,31×106 CFU/ml.Kata kunci: bakteri ureolitik, produktivitas, sedimen mangrove, Muara Sungai Citarum
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Parida, Ida, Tri Asmira Damayanti, and Giyanto Giyanto. "Isolasi, Seleksi, dan Identifikasi Bakteri Endofit sebagai Agens Penginduksi Ketahanan Padi terhadap Hawar Daun Bakteri." Jurnal Fitopatologi Indonesia 12, no. 6 (March 8, 2017): 199. http://dx.doi.org/10.14692/jfi.12.6.199.
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Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae is one of the major problems in rice production in Indonesia. One of the control measures for the disease is by the utilization of endophytic bacteria. This study was aimed to determine the ability of endophytic bacteria isolated from the roots, stems and leaves of rice in inducing plant resistance to bacterial leaf blight on rice. Screening of endophytic bacteria showed that 370 isolates have good viability and have different colony morphology. Among them, 7 isolates were able to induce resistance and 1 isolate was able to promote the growth of rice in the nursery. However, only 8 isolates did not cause hypersensitive reaction on tobacco plants. The selected isolates of endophytic bacteria were further examined for their ability to induce resistance of rice in greenhouse experiments. Observation involved several variables, i.e. PR1 and PBZ1 gene expression, peroxidase enzyme activity, incubation period, and disease progression. Seven isolates of endophytic bacteria were able to induce PR1 and PBZ1 gene expression, 4 isolates were able to increase peroxide enzyme activity, 4 isolates could prolong the incubation period, and 2 isolates can inhibit the development of disease. However, only EB4 451 isolate was consistently able to induce PR1 and PBZ1 gene expression, increased peroxide enzyme activity, prolonged incubation period, and suppressed the progression of the disease. The EB4 451 isolate was identified based on nucleotide sequence as Bacillus subtilis.
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Syahfitri, Dian, Nisa Rachmania Mubarik, and Lisdar A. Manaf. "Penggunaan Bakteri Kitinolitik sebagai Pengendali Hayati Colletotrichum capsici pada Tanaman Cabai." Jurnal Fitopatologi Indonesia 14, no. 4 (November 22, 2018): 120. http://dx.doi.org/10.14692/jfi.14.4.120.
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Use of Chitinolytic Bacteria as Biological Control of Colletotrichum capsici on Chili PlantsColletotrichum capsici is known as the causal agent of anthracnose disease in chili plant and may cause reduction of crop yield. Chitinolytic bacteria, namely Serratia marcescens KAHN 15.12, Bacillus thuringiensis SAHA 12.12, and BAE 36 were reported to have antagonistic activity against C. capsici. Therefore, a study was conducted to determine the potential of chitinolytic bacteria on controlling C. capsici on chili plants in greenhouse experiment. Three bacterial isolates used as biocontrol agent was formulated by using talcum as carrier materials. The methodologies consisted of characterization of bacterial isolates, formulation of biocontrol agent, viability test of bacterial isolate, efficacy of biocontrol agents in the laboratory and in the greenhouse. Disease severity in the laboratory reached 64% when chili treated with isolate formulation of BAE 36. In the greenhouse, BAE 36 isolate formulation and consortium formulation were able to suppress infection of C. capsici; each was indicated by disease incidence of 25% and 50%, respectively. These results indicated that chitinolytic bacterial formulations could be potencial as biocontrol agents of C. capsici.
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Prihatiningsih, Nur, Heru Adi Djatmiko, and Puji Lestari. "SCREENING OF COMPETENT RICE ROOT ENDOPHYTIC BACTERIA TO PROMOTE RICE GROWTH AND BACTERIAL LEAF BLIGHT DISEASE CONTROL." Jurnal Hama dan Penyakit Tumbuhan Tropika 20, no. 1 (April 26, 2020): 78–84. http://dx.doi.org/10.23960/j.hptt.12078-84.
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Screening of competent rice root endophytic bacteria to promote rice growth and bacterial leaf blight disease control. This study was aimed to collect isolate endophytic bacterial of rice roots which able to produce IAA, determine the effect of endophytic bacteria application in stimulating rice plant growth, and evaluate the potential of rice root endophytic bacteria for controlling bacterial leaf blight. This reasearch was carried out at the Screen House, Plant Protection Laboratory, and Agrohorti Laboratory of the Agriculture Faculty, Jenderal Soedirman University. Isolation of rice root endophytic bacteria was carried out by purposive random sampling from several marginal lands. The results showed that 8 isolates of rice root endophytic bacteria were able to produce IAA, ranging from 57.56 to 79.33 ppm and B07 isolate from Serayu produced the highest amount of IAA. The B04 and B07 isolates were contributed to increase the rice plant growth. The application of rice root endophytic bacteria was effective in controlling bacterial leaf blight.
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Serdani, Army Dita, Luqman Qurata Aini, and Abdul Latief Abadi. "ISOLASI DAN IDENTIFIKASI BAKTERI ENDOFIT DARI TANAMAN PADI (Oryza sativa) SEBAGAI PENGENDALI PENYAKIT HAWAR DAUN BAKTERI AKIBAT Xanthomonas oryzae pv. oryzae." VIABEL: Jurnal Ilmiah Ilmu-Ilmu Pertanian 12, no. 1 (May 16, 2018): 18–26. http://dx.doi.org/10.35457/viabel.v12i1.422.
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Rice cultivation often face obstacles, and one of them is bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo). The application of endophytic bacteria is one of solutions to overcome this problem. Endophytic bacteria are non-pathogenic bacteria, which live in plant tissues. These bacteria could be isolated from the plant tissues. They may adapt to the plant tissues and produce antibiosis that could increase the plant resistance. Therefore, objectives of the research were to isolate the endophytic bacteria from healthy plant tissues and to identify them through morphology, physiology, biochemistry, and molecular. Method of the research was explorative along with three principal activities, such as isolation, selection, and identification on the potential endophytic bacteria. The isolation of the endophytic bacteria from healthy rice tissues has resulted 53 isolates and five of them have antagonistic ability in vitro against Xoo. Isolate ak9 has the highest antagonistic ability, 7.67 mm, in comparison with other isolates. Results of identification showed that those five potential bacteria have close relations, such as da3 with Bacillus cereus, isolate ak9 with Burkholderia sp., isolate ak30 with Enterobacter sp and isolate da9, as well as ak15 are Corynebacterium sp.
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Serdani, Army Dita, Luqman Qurata Aini, and Abdul Latief Abadi. "ISOLASI DAN IDENTIFIKASI BAKTERI ENDOFIT DARI TANAMAN PADI (Oryza sativa) SEBAGAI PENGENDALI PENYAKIT HAWAR DAUN BAKTERI AKIBAT Xanthomonas oryzae pv. oryzae." VIABEL: Jurnal Ilmiah Ilmu-Ilmu Pertanian 12, no. 1 (May 16, 2018): 18–26. http://dx.doi.org/10.30957/viabel.v12i1.422.
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Rice cultivation often face obstacles, and one of them is bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo). The application of endophytic bacteria is one of solutions to overcome this problem. Endophytic bacteria are non-pathogenic bacteria, which live in plant tissues. These bacteria could be isolated from the plant tissues. They may adapt to the plant tissues and produce antibiosis that could increase the plant resistance. Therefore, objectives of the research were to isolate the endophytic bacteria from healthy plant tissues and to identify them through morphology, physiology, biochemistry, and molecular. Method of the research was explorative along with three principal activities, such as isolation, selection, and identification on the potential endophytic bacteria. The isolation of the endophytic bacteria from healthy rice tissues has resulted 53 isolates and five of them have antagonistic ability in vitro against Xoo. Isolate ak9 has the highest antagonistic ability, 7.67 mm, in comparison with other isolates. Results of identification showed that those five potential bacteria have close relations, such as da3 with Bacillus cereus, isolate ak9 with Burkholderia sp., isolate ak30 with Enterobacter sp and isolate da9, as well as ak15 are Corynebacterium sp.
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RINA, NURFITRIANI, NI PUTU RATNA AYU KRISHANTI, ALINA AKHDIYA, and ARIS TRI WAHYUDI. "Penapisan Bakteri Filosfer Penghasil Senyawa Bioaktif Anti Xanthomonas oryzae pv. oryzae Penyebab Penyakit Hawar Daun Bakteri pada Padi." Jurnal Sumberdaya Hayati 2, no. 1 (November 14, 2016): 19–24. http://dx.doi.org/10.29244/jsdh.2.1.19-24.
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Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the important diseases in rice crops in Indonesia. The disease is difficult to be controlled since it attacks the rice plant at different growth stages such as tillering, flowering and ripening. One of the alternatives that could be used to control the disease is by using phyllosphere bacteria as the biocontrol agents. This study aims to isolate, characterize and screen the rice phyllosphere bacteria producing bioactive compounds against Xoo. Phyllosphere bacteria isolated from healthy leaves of rice var. Ciherang by using 4 different media obtained 285 bacterial isolates which were consisted of the 65 isolates of King’s B agar, 86 isolates of Nutrient agar, 81 isolates of Luria-Bertani agar, and 53 isolates of Trypticase Soy agar media. Antagonist test using double layer method showed 58 isolates of phyllosphere bacteria produced bioactive compounds that inhibited the growth of Xoo. Pathogenicity test agaist rice leaf revealed 18 bacterial isolates did not perform their potencies as pathogenic bacteria. Among the 18 non-phytopathogenic bacterial isolates, 14 isolates belong to Gram-positive bacteria and 4 isolates belong to Gram-negative bacteria. Five isolates among Gram positive bacteria were predicted as Bacillus genera.
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Wijayanti, Marini, Dade Jubaedah, Januar Ahlan Suhada, Siti Yuliani, Nabilah Saraswati, Tanbiyaskur, Mochamad Syaifudin, and Hary Widjajanti. "DNA Barcoding of Swamp Sediment Bacterial Isolates for Swamp Aquaculture Probiotic." E3S Web of Conferences 68 (2018): 01023. http://dx.doi.org/10.1051/e3sconf/20186801023.
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Bacteria derived from swamp sediments and aquaculture ponds potential to be probiotics. The study aims to determine the sequence of 16S r RNA gene of isolate of probiotics candidate bacteria from sediment of swamp, to determine the phylogenetic tree between the bacterial species from isolates and Gene Bank data central for their potential as probiotic. The samples of bacteria resulted from pure isolation selected from the sediment of pond cultivation and swamp waters at Lebung Karangan Reservation, Ogan Ilir Regency, Indralaya, South Sumatra. This study was started from cultivating bacteria, extracting DNA of bacteria, amplification 16S r RNA genes by PCR, running electrophoresis, and sequencing the amplicon for determining DNA barcodes of bacteria from sediment of swamp and rearing pond. The result of BLAST analysis showed that KA isolate had the highest similarity 97% with Streptomyces sp. Hjorring101 from Denmark and RA isolate had the highest similarity 98% with Streptomyces sp. BD99 from Pakistan. KE isolate had the highest similarity 99% with Bacillus subtilis CESi5 from Japan and RE isolate had the highest similarity 93% with Bacillus sp.2bFR from Manado. All of isolates were bacteria potentially as swamp aquaculture probiotics.
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Fuji Arriani, Intan, Abdul Latief Abadi, and Luqman Qurata Aini. "KARAKTERISASI BAKTERI PATOGEN PENYEBAB LAYU PADA TANAMAN BAWANG MERAH (Allium ascalonicum L.)." VIABEL: Jurnal Ilmiah Ilmu-Ilmu Pertanian 14, no. 1 (May 30, 2020): 69–75. http://dx.doi.org/10.35457/viabel.v14i1.1004.
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Shallot (Allium ascalonicum L.) is one of the vegetable commodities in the form of tubers that have a high economic value. The development of shallot cultivation in Indonesia often experiences obstacles, one of which is an obstacle in the process of shallot cultivation, namely the attack of Plant Disturbing Organisms (OPT). Information about diseases caused by pathogenic bacteria is still very limited. This study aims to determine the symptoms and identification of pathogenic bacteria that cause wilt in shallots. Bacterial isolation was carried out using Nutrient Agar (NA) media and 36 bacterial isolates were collected from shallots. Bacterial isolates were then tested for pathogenicity to determine the ability of bacteria to cause wilt disease in shallots. The results of isolation obtained 10 bacterial isolates that can show symptoms on red onions namely wilted leaves, yellow and soft rotten tubers. Four isolates including positive can show hypersensitivity symptoms, namely M11, N20, N17 and N14. Based on the identification of bacteria in physiology showed 2 groups of different isolates. Biochemical test results of Isolate M11, N20 and show species suspected of B. cepacia. N3 and N14 isolates are suspected to be E. carotavora subsp. carotavora. The bacterial isolates N7, N17, P5 and P7 were suspected to be E. carotavora subsp. betavasculorum. The isolate of N4 bacteria is suspected to be E. cacticida.
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Prihanto, Asep Awaludin, Rahmi Nurdiani, Hidayatun Muyasharoh, and Jauharotul Afifah. "Identification of Protease-Producing Halophilic Bacteria Isolated from Salt-Pond Soil." Jurnal Ilmiah Perikanan dan Kelautan 12, no. 1 (March 21, 2020): 181. http://dx.doi.org/10.20473/jipk.v12i1.14725.
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HighlightsThe halophilic bacteria were isolated from salt-pond soil.Isolate C2 was identified as the best protease producer.Isolate C2 was identified as B. amyloliquefaciens strain UBCAbstractProtease is an important enzyme for various industries, such as pharmaceuticals, leather production, meat processing, protein hydrolyzate, food products, and waste processing industries. This study aimed to isolate and identify protease-producing bacteria isolated from salt-pond soil at the Tuban Regency, East Java, Indonesia. Bacterial communities in the soil samples were firstly isolated from the soil samples by culture-dependent technique on Luria Bertani agar enriched with 5% NaCl. Thereafter, bacterial colonies that grew on the media were purified and screened for their protease production using a skim-milk agar. The bacterial colony which produced protease was further identified using phenotypic (gram staining) and genotypic assays (the 16S rDNA sequence). The result showed that one isolate out of six (isolate C2) obtained from the soil sample was observed to produce a protease enzyme. Based on its 16S rDNA sequence, the isolate was identified as Bacillus amyloliquefaciens strain UBC. These results suggest that B. Amyloliquefaciens strain UBC is a salt-tolerant bacterium (halophilic bacteria) which has the potential to be further developed for protease-producing biological agents.
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KHALILA, RUHUL, Lenni Fitri, and SUHARTONO SUHARTONO. "Isolation and Characterization of Thermophilic Bacteria as Cellulolytic Enzyme Producer from the Hot Spring of Ie Seuum Aceh Besar, Indonesia." Microbiology Indonesia 14, no. 1 (August 11, 2020): 4. http://dx.doi.org/10.5454/mi.14.1.4.
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Cellulase enzymes can be isolated from thermophile bacteria obtained from the hot spring Ie Seuum, Aceh Besar. This research aimed to recover and characterize the isolates morphologically and biochemically followed by determination of the thermophile bacterial isolates potential as cellulolytic enzyme producers,. The sampling method in this research was conducted by a purposive sampling at temperature of 70 oC, 60 oC and 50 oC. Isolation of thermophilic bacteria was carried out on nutrient agar (NA) media. There were four isolates of thermophilic bacteria isolated recovered at 70 oC, five isolates at 60 oC, and seven isolates at 50 oC. Of the 18 isolates obtained, 15 of them were able to produce cellulase enzymes. Cellulase enzyme production can be determined by the presence of clear zones around bacterial colonies on CMC media after addition of 1% congo red drops and wash with 1 M NaCl. The highest five Cellulolytic Index (CI) values were obtained from isolates ISB75; ISB64; ISB52; ISB54; ISB56 that were 1.23; 2.22; 1.39; 1.59; 1.10, respectively. Biochemical tests carried out on 5 isolates with the highest cellulolytic index values showed that the bacterial isolate were suspected to be from the genera of Bacillus sp.
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Hutchens, E., E. Valsami-Jones, S. McEldowney, W. Gaze, and J. McLean. "The role of heterotrophic bacteria in feldspar dissolution – an experimental approach." Mineralogical Magazine 67, no. 6 (December 2003): 1157–70. http://dx.doi.org/10.1180/0026461036760155.
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AbstractThis paper presents the results of a laboratory study on the influence of heterotrophic bacteria on dissolution of a silicate mineral (K-feldspar) under a variety of growth conditions. Twenty seven strains of heterotrophic bacteria were isolated from a feldspar-rich soil (Shap, NW England). Liquid and solid minimal aerobic media (C/N-sufficient, K-limited, Fe-limited, N-limited and glucose/NH4Cl only) at 26ºC were used for isolation of the bacteria. The media selected bacterial isolates that were fastgrowing aerobic heterotrophs able to use glucose as the sole source of carbon and energy. The extent of mineral dissolution (in the presence of the isolates) was assessed after 48 h of incubation by measuring the release of Al from the K-feldspar by ICP-AES. More detailed dissolution experiments were carried out with one of the strains, Serratia marcescens, an isolate that was very effective in enhancing feldspar dissolution. The main conclusions of this study are: (1) the degree of enhancement of K-feldspar dissolution varied with bacterial isolate and growth conditions; (2) enhancement of dissolution began during stationary phase growth; (3) the production of chelating compounds (exopolymers, siderophores, pigments) during the stationary phase might be a possible mechanism for bacterially enhanced K-feldspar dissolution; (4) the frequent sub-culturing of isolates can have a significant effect on their physiological characteristics and may possibly influence their capacity to enhance mineral dissolution.
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Putri, Dwi Hilda, Mades Fifendy, and Moca Faulina Putri. "DIVERSITY OF BACTERIAL ENDOPHYTES IN IN YOUNG AND OLD LEAVES OF ANDALEH PLANT (MORUS MACROURA MIQ.)." EKSAKTA: Berkala Ilmiah Bidang MIPA 19, no. 1 (April 28, 2018): 125–30. http://dx.doi.org/10.24036/eksakta/vol19-iss1/122.
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Endphytic microbes are microscopic living organisms (bacteria and fungi) that live in plant tissues (xylem and phloem), leaves, roots, fruit, and stems. These microbes live symbiosis of mutual benefit. Morus macroura miq. or Andaleh is a native plant of western Sumatra that is considered rare. The purpose of this study was to isolate and identify entophytic bacteria found in young leaves and old leaves andaleh taken from the village Andaleh, Kec. Batipuah West Sumatra. The methods used to isolate endophytic bacteria from andaleh leaves are spread plate and streak plate. Identification of endophytic bacteria in macroscopic or colony morphology. A total of 12 isolates of endophytic bacteria from young andaleh leaf and 8 isolates of endophytic bacteria from andaleh leaf. Identification Microscopic using gram staining obtained bacteria 18 gram positive endophytic bacterial isolates and 2 isolate gram negative bacteria.
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Abdi, Reta D., Barbara E. Gillespie, Susan Ivey, Gina M. Pighetti, Raul A. Almeida, and Oudessa Kerro Dego. "Antimicrobial Resistance of Major Bacterial Pathogens from Dairy Cows with High Somatic Cell Count and Clinical Mastitis." Animals 11, no. 1 (January 8, 2021): 131. http://dx.doi.org/10.3390/ani11010131.
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Mastitis is the most prevalent and economically important disease caused by different etiological agents, which leads to increased somatic cell count (SCC) and low milk quality. Treating mastitis cases with antimicrobials is essential to reduce SCC and improve milk quality. Non-prudent use of antimicrobials in dairy farms increased the development of antimicrobial resistant bacteria. This study’s objectives were (1) to isolate and identify etiological agents of mastitis and (2) to determine antimicrobial resistance profiles of bacterial isolates. A total of 174 quarter milk samples from 151 cows with high SCC and clinical mastitis from 34 dairy farms in Tennessee, Kentucky, and Mississippi were collected. Bacterial causative agents were determined by bacteriological and biochemical tests. The antimicrobial resistance of bacterial isolates against 10 commonly used antimicrobials was tested. A total of 193 bacteria consisting of six bacterial species, which include Staphylococcus aureus, Streptococcus uberis, Streptococcus dysgalactiae, Escherichia coli, Klebsiella oxytoca and Klebsiella pneumoniae were isolated. Staphylococcus aureus was the predominant isolate followed by Strep. spp., E. coli, and Klebsiella spp. Results of this study showed that Gram-negatives (E. coli and Klebsiella spp.) were more resistant than Gram-positives (Staph. aureus and Streptococcus spp.). Continuous antimicrobial resistance testing and identification of reservoirs of resistance traits in dairy farms are essential to implement proper mitigation measures.
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Abdi, Reta D., Barbara E. Gillespie, Susan Ivey, Gina M. Pighetti, Raul A. Almeida, and Oudessa Kerro Dego. "Antimicrobial Resistance of Major Bacterial Pathogens from Dairy Cows with High Somatic Cell Count and Clinical Mastitis." Animals 11, no. 1 (January 8, 2021): 131. http://dx.doi.org/10.3390/ani11010131.
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Mastitis is the most prevalent and economically important disease caused by different etiological agents, which leads to increased somatic cell count (SCC) and low milk quality. Treating mastitis cases with antimicrobials is essential to reduce SCC and improve milk quality. Non-prudent use of antimicrobials in dairy farms increased the development of antimicrobial resistant bacteria. This study’s objectives were (1) to isolate and identify etiological agents of mastitis and (2) to determine antimicrobial resistance profiles of bacterial isolates. A total of 174 quarter milk samples from 151 cows with high SCC and clinical mastitis from 34 dairy farms in Tennessee, Kentucky, and Mississippi were collected. Bacterial causative agents were determined by bacteriological and biochemical tests. The antimicrobial resistance of bacterial isolates against 10 commonly used antimicrobials was tested. A total of 193 bacteria consisting of six bacterial species, which include Staphylococcus aureus, Streptococcus uberis, Streptococcus dysgalactiae, Escherichia coli, Klebsiella oxytoca and Klebsiella pneumoniae were isolated. Staphylococcus aureus was the predominant isolate followed by Strep. spp., E. coli, and Klebsiella spp. Results of this study showed that Gram-negatives (E. coli and Klebsiella spp.) were more resistant than Gram-positives (Staph. aureus and Streptococcus spp.). Continuous antimicrobial resistance testing and identification of reservoirs of resistance traits in dairy farms are essential to implement proper mitigation measures.
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Woźniak, M., A. Gałązka, J. Grządziel, and M. Głodowska. "The identification and genetic diversity of endophytic bacteria isolated from selected crops." Journal of Agricultural Science 156, no. 4 (May 2018): 547–56. http://dx.doi.org/10.1017/s0021859618000618.
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AbstractA collection of 45 isolates was created based on bacteria isolated from maize, broad bean, wheat, rye and wild plants such as horsetail and burdock. The aim of the current study was to isolate the bacteria, and then identify and assess the degree of genomic diversity. The molecular identification of microsymbionts isolated from the endosphere (root and stem) of plants grown in agricultural soils was performed using 16S rRNA gene sequencing. To evaluate the genomic diversity between strains that occurred in multiple host plants, 18 bacterial isolates representing four species were subjected to denaturing gradient gel electrophoresis. The 16S rDNA analysis assigned all bacterial isolates to ten genera, from whichRhizobiumwas represented by 19 isolates,Delftiaby 11,Agrobacteriumby five,Stenotrophomonasby three,Brevundimonasby two andNovosphingobium,Variovorax, Collimonas, AchromobacterandComamonasby only one isolate. Furthermore, the genomic diversity of the 11 isolates ofDelftiasp. was assessed using the BOX – polymerase chain reaction (BOX-PCR) and enterobacterial repetitive intergenic consensus – PCR (ERIC-PCR) methods. Typing patterns and analysis using BOX-PCR and ERIC-PCR data demonstrated similarities among the tested isolates. In general, the results obtained with BOX-PCR and ERIC-PCR were in good agreement. However, a greater degree of differentiation patterns of the genomic DNA was obtained in the ERIC-PCR method.
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Begum, Kohinur, Sultana Juhara Mannan, Refaya Rezwan, Md Mahinur Rahman, Md Shajidur Rahman, and Alam Nur E. Kamal. "Isolation and Characterization of Bacteria with Biochemical and Pharmacological Importance from Soil Samples of Dhaka City." Dhaka University Journal of Pharmaceutical Sciences 16, no. 1 (July 30, 2017): 129–36. http://dx.doi.org/10.3329/dujps.v16i1.33390.
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We studied soil samples from Dhaka municipality area to isolate and characterize bacteria having potential biochemical and pharmacological importance. Total twenty five soil samples were collected from fish, vegetables and fruits dump area from Dhaka City. Bacterial population was sub-cultured in trypticase soya agar (TSA) plate. Nineteen colonies were isolated, cultured and characterized by gram staining and biochemical tests. Six isolates were found to be gram negative while thirteen were gram positive. All isolates were positive in oxidase, catalase, citrate, and protease tests. Eight isolates showed coagulase negative and nine were coagulase positive. It was found that all bacterial isolates were sensitive to tetracycline, chloramphenicol, gentamycin, ciprofloxacin, azithromycin and ceftriaxone. About 95% of the bacterial isolates were resistant to penicillin-G and ampicillin. About 89%, 26%, 21% and 11% of the bacterial isolates were resistant to amoxicillin, co-trimoxazole, nalidixic acid and erythromycin, respectively. It was found that bacterial isolates produce chemical(s) inhibitory to other bacterial strains including both gram positive and gram negative bacteria. Further studies are needed to characterize the potential antibacterial factor(s) and other bioactive compound (s) present in these bacterial isolates from soil samples.Dhaka Univ. J. Pharm. Sci. 16(1): 129-136, 2017 (June)
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Ryandini, Dini, Hendro Pramono, and Sukanto Sukanto. "Antibacterial Activity of Streptomyces SAE4034 Isolated from Segara Anakan Mangrove Rhizosphere against Antibiotic Resistant Bacteria." Biosaintifika: Journal of Biology & Biology Education 10, no. 1 (April 2, 2018): 117–24. http://dx.doi.org/10.15294/biosaintifika.v10i1.12896.
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Actinomycetes SAE4034 isolates was isolated from Rhizophora apiculata rhizosphere mud showed some antibacterial properties. The antibacterial ability of this isolate has not been tested on antibiotic resistant bacterial pathogens. However, there was no research has been reported regarding actinomycetes from Segara Anakan mangrove area resulting compounds inhibit the growth of antibiotics-resistant bacteria. Therefore, it is important to investigate its capability against antibiotics resistant bacteria or multi drug resistant bacteria (MDR bacteria). The research aimed to know the ability of actinomycetes SAE4034 in inhibit MDR bacteria and to identify the species profiles. The research methods included isolate characterization involving morphology, physiology/enzymatic and molecular properties. MDR bacterial inhibition assay, antibacterial compound extraction and antibacterial compound test using thin layer chromatography (TLC) method, observation of morphological and biochemical properties, DNA isolation, amplification and analysis of 16SrRNA sequence, and phylogeny tree analysis. The methods of this study included MDR anti-bacterial assay and antibacterial compound test. Subsequent step was isolate characterization including observation of morphological and physiological / enzymatic properties, and 16S rRNA gene sequence. The results showed that culture extract was able to inhibit the growth of MDR bacteria i.e. Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus sp., but no inhibition to Enterobacter cloacae. The bioactive compound showed 4 spots with Rf values of 0.36; 0.45; 0.54; and 0.6. Based on morphology, physiology / enzymatic and 16S rRNA gene sequences characteristics, actinomycetes SAE4034 isolate is Streptomyces sp. This research showed new Streptomyces strain that serves as a source of MDR antibacterial compounds and useful in development of antibiotic for combating infectious diseases caused by MDR bacteria
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Riyanto, Julius, and Sogandi. "Investigating the anti-acne potential of endophytic bacterial extracts isolated from Mangifera casturi in indigenous South Borneo, Indonesia." Journal of Agriculture and Applied Biology 1, no. 2 (December 24, 2020): 54–63. http://dx.doi.org/10.11594/jaab.01.02.03.
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Endophytic bacteria are beneficial microorganisms living in the tis-sues system of various parts of a plant, including fruits, leaves, twigs, and roots. The bacteria are stated as forming colonies without caus-ing any damage to the plant. Thus, this study aimed at isolating endo-phytic bacterial from the leaves, barks, and fruits of Kasturi plant (Mangifera casturi), screening its endophytic bacteria, determining the potential of those endophytic bacteria, identifying 16S rRNA and analyzing potential growth of the bacteria. The isolated endophytic bacteria appeared to show potential activity against pathogenic bac-teria Propionibacterium acnes with disc-diffusion methods. Besides, the observations on bacterial activities showed isolate L2, S2 and F4 isolated from leaves, bark and fruits, respectively, as the most potent producers of antibacterial compounds. Technically, those activities were indicated by the formation of clear zones. Molecular identifica-tion was investigated by applying PCR amplification on 16S rRNA gene. Furthermore, the isolate L2 was identified as Enterobacter clo-acae with 99% sequence similarities; however, isolates S2 and F4 were identified as Escherichia coli. Therefore, these findings sug-gested that the identified strains would contribute to any further searches for new sources of anti-acne substances.
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Doumbou, Cyr Lézin, Vladimir Akimov, and Carole Beaulieu. "Selection and Characterization of Microorganisms Utilizing Thaxtomin A, a Phytotoxin Produced byStreptomyces scabies." Applied and Environmental Microbiology 64, no. 11 (November 1, 1998): 4313–16. http://dx.doi.org/10.1128/aem.64.11.4313-4316.1998.
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ABSTRACT Thaxtomin A is the main phytotoxin produced by Streptomyces scabies, a causal agent of potato scab. Thaxtomin A is a yellow compound composed of 4-nitroindol-3-yl-containing 2,5-dioxopiperazine. A collection of nonpathogenic streptomycetes isolated from potato tubers and microorganisms recovered from a thaxtomin A solution were examined for the ability to grow in the presence of thaxtomin A as a sole carbon or nitrogen source. Three bacterial isolates and two fungal isolates grew in thaxtomin A-containing media. Growth of these organisms resulted in decreases in the optical densities at 400 nm of culture supernatants and in 10% reductions in the thaxtomin A concentration. The fungal isolates were identified as aPenicillium sp. isolate and a Trichoderma sp. isolate. One bacterial isolate was associated with the speciesRalstonia pickettii, and the two other bacterial isolates were identified as Streptomyces sp. strains. The sequences of the 16S rRNA genes were determined in order to compare thaxtomin A-utilizing actinomycetes to the pathogenic organism S. scabies and other Streptomyces species. The nucleotide sequences of the γ variable regions of the 16S ribosomal DNA of both thaxtomin A-utilizing actinomycetes were identical to the sequence of Streptomyces mirabilis ATCC 27447. When inoculated onto potato tubers, the three thaxtomin A-utilizing bacteria protected growing plants against common scab, but the fungal isolates did not have any protective effect.
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Carrim, Aysha Jussara Ivonilde, Edweis Cândida Barbosa, and José Daniel Gonçalves Vieira. "Enzymatic activity of endophytic bacterial isolates of Jacaranda decurrens Cham. (Carobinha-do-campo)." Brazilian Archives of Biology and Technology 49, no. 3 (May 2006): 353–59. http://dx.doi.org/10.1590/s1516-89132006000400001.
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The objectives of this work was to isolate endophytic bacteria from Jacaranda decurrens Cham. and screening of some enzymes of biotechnological interest. Ten (10) bacterial species were isolated and identified from the leaves and steams. All the isolates presented enzymatic activity, which was ranked as follows: proteolytic (60%) and amilolytic activity (60%), lipolytic (40%), esterasic (40%). However, cellulolytic and pectinolytic activities were not detected. This is the first report on the isolation and identification of endophytic bacteria from Jacaranda decurrens Cham.
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Gryndler, Milana, and Hana Hršelová. "Isolation of bacteria from ectomycorrhizae of Tuber aestivum Vittad." Acta Mycologica 47, no. 2 (December 23, 2013): 155–60. http://dx.doi.org/10.5586/am.2012.018.
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Fifteen different cultivation media were used to isolate bacteria with the idea to obtain taxa specifically associated with ectomycorrhizae of <em>Tuber aestivum</em>. Ectomycorrhizae were collected at the sampling points previously analyzed for bacterial molecular diversity. We isolated 183 bacterial strains and identified them on the basis of the partial sequence of 16S rDNA. Out of these isolates, only 4 corresponded to operational taxonomic units significantly associated with <em>T. aestivum</em> ectomycorrhizae in previous molecular study. Preliminary study of the effect of 12 selected isolates on growth of T. aestivum mycelium showed no stimulation and one isolate induced the damage of hyphae. Different isolation strategy has to be developed to increase the probability of cultivation of potentially important components of <em>T. aestivum</em> mycorrhizosphere.
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Ropiatningsuari, Nika, Suryo Wiyono, and Suryahadi Suryahadi. "Skrining Bakteri Dekomposer Sebagai Penghilang Bau Kotoran Ayam." Bumi Lestari Journal of Environment 18, no. 1 (February 1, 2018): 19. http://dx.doi.org/10.24843/blje.2018.v18.i01.p03.
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The decomposition of chicken excreta produce odorous gases, that case environmental pollution. One of alternative technique to reduce the odorous gases is by applying specific bacteria. The aim of this study was to obtain bacterial isolates that capable of reducing NH3 and H2S production and odors. Decomposer bacterial candidates were isolated from chicken manure, guano, and peat soil. Selection of isolates of oxidizing bacteria is carried out using selective media. All isolates that were found from chicken manure, guano, and peat soil tested for hypersensitive reaction on tobacco and hemolysis on blood agar. The isolated bacteria that showed negative HR and HL responses than used for a further experiment. Five isolates of bacteria which reduce odors based on organoleptic test were WiK 15, TnK 7, WiGu 11, CGu 7 and MaGa 5. NH3 and H2S from decomposition chicken excreta were analyzed by spectrophotometric and colorimetric method. The average of total NH3 production from seven days observation showed ranged level from 1.09 ppm to 1.77 ppm, while total H2S gas production ranged from 15.05 to 16.57 ppm. Bacterial isolate CGU 7 showed make lowest total production of NH3.
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Al-Karablieh, Nehaya. "Antimicrobial Activity of Bacillus Persicus 24-DSM Isolated from Dead Sea Mud." Open Microbiology Journal 11, no. 1 (December 29, 2017): 372–83. http://dx.doi.org/10.2174/1874285801711010372.
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Intorduction:Dead Sea is a hypersaline lake with 34% salinity, gains its name due to the absence of any living macroscopic creatures. Despite the extreme hypersaline environment, it is a unique ecosystem for various halophilic microorganisms adapted to this environment.Aims & Objectives:Halophilic microorganisms are known for various potential biotechnological applications, the purpose of the current research is isolation and screening of halophilic bacteria from Dead Sea mud for potential antimicrobial applications.Methods & Materials:Screening for antagonistic bacteria was conducted by bacterial isolation from Dead Sea mud samples and agar plate antagonistic assay. The potential antagonistic isolates were subjected to biochemical characterization and identification by 16S-rRNA sequencing. Among the collected isolates, four isolates showed potential antagonistic activity againstBacillus subtilis6633 andEscherichia coli8739. The most active isolate (24-DSM) was subjected for antagonistic activity and minimal inhibitory concentration against different gram positive and negative bacterial strains after cultivation in different salt concentration media. Results: The results of 16S-rRNA analysis revealed that 24-DSM is very closely related toBacillus persicusstrain B48, which was isolated from hypersaline lake in Iran.Conclusion:Therefore, the isolate 24-DSM is assigned as a new strain ofB. persicusiisolated from the Dead Sea mud.B. persicusi24-DSM showed higher antimicrobial activity, when it was cultivated with saline medium, against all tested bacterial strains, where the most sensitive bacterial strain wasCorynebacterium diphtheria51696.
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Joshi, Navneet, Laxmi Choudhary, Kanti Prakash Sharma, and Hafiz M. N. Iqbal. "PLANT GROWTH POTENTIAL OF SALT TOLERANT ENDOPHYTE Pseudomonas Sihuiensis ISOLATED FROM CHICKPEA." Journal of Experimental Biology and Agricultural Sciences 9, no. 2 (April 25, 2021): 231–38. http://dx.doi.org/10.18006/2021.9(2).231.238.
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Bacterial endosymbionts are well characterized for plant growth promotion. In this study, the root, nodules, and stem of the Cicer arietinum crop planted in a semi-arid zone were used as a source to isolate potential plant growth bacteria. The ability to grow under salt stress was determined, and the potential isolate was screened for plant growth promotion traits. The selected isolate was identified by the 16S rDNA method. Pot trials were conducted to know the ability of the isolate to promote plant growth in-vivo. Among various isolates obtained, a bacterial isolate obtained from root showed the ability to grow in the presence of 10 % Sodium fluoride (NaF). The isolate produced Indole Acetic acid in an amount of 72 mg per liter in production medium. The bacteria solubilized phosphate and produce exopolysaccharide (2.12 g per liter). The isolate was identified as Pseudomonas sihuiensis. The result of pot trials reveals that the endophyte promotes plant growth under stress conditions and may be used as a bio-fertilizer.
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Chigerwe, Munashe, Vengai Mavangira, Barbara A. Byrne, and John A. Angelos. "Antibiotic resistance patterns of bacteria isolated from indwelling Foley catheters following tube cystostomy in goats with obstructive urolithiasis." Journal of Veterinary Diagnostic Investigation 29, no. 3 (March 28, 2017): 316–20. http://dx.doi.org/10.1177/1040638717695607.
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Tube cystostomy is a surgical method used for managing obstructive urolithiasis and involves placement of a Foley catheter into the urinary bladder. We identified and evaluated the antibiotic resistance patterns of bacteria isolated from indwelling Foley catheters following tube cystostomy in goats with obstructive urolithiasis. Urine samples collected over a 10-y period from catheter tips at the time of removal were submitted for bacteriologic culture and antibiotic susceptibility testing. Resistance patterns to antibiotics, trends in the resistance patterns over the study period, and the probability of a bacterial isolate being resistant as a function of the identity of the isolate and antibiotic tested were determined. A total of 103 urine samples from 103 male goats with obstructive urolithiasis managed surgically with tube cystostomy were included in the study. Aerococcus (36.9%) and Enterococcus (30.1%) were isolated most frequently. The susceptibility patterns of all bacteria isolated did not change over the study period ( p > 0.05). Proportions of isolates resistant to 1, 2, and ≥3 antibiotics were 36.9%, 18.5%, and 23.3%, respectively. Thus, 41.8% of bacterial isolates were resistant to 2 or more antibiotics tested. The probability of Aerococcus spp., Escherichia coli, and Pseudomonas aeruginosa isolates to be resistant to ampicillin, ceftiofur, erythromycin, penicillin, or tetracycline ranged from 0.59 to 0.76.
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Meliah, Siti, Annisa Wahyu Hardiyanti, Ni’ma Haida, Gita Azizah Putri, and Erny Qurotul Ainy. "Penapisan Bakteri Penghambat Fusarium yang Diisolasi dari Cairan Kantung Semar (Nepenthes sp.)." Jurnal Ilmu Pertanian Indonesia 25, no. 4 (October 27, 2020): 627–35. http://dx.doi.org/10.18343/jipi.25.4.627.
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The genus Fusarium sp. is a pathogenic fungal for many cultivated plants. The bacteria isolated from monkey cup (Nepenthes sp.) fluid possess the ability to produce hydrolytic enzyme, such as chitinase which can be utilized to inhibit the growth of mycelia of pathogenic fungi. The aims of this study are to isolate bacteria from monkey cup liquid, to test their abilities to produce protease, chitinase, and cellulase, as well as their abilities to inhibit Fusarium. The bacteria were isolated using serial dilution method on Reasoner’s 2A agar medium. Enzymatic activities of bacterial isolates were determined by inoculating them on tested medium supplemented with casein protein, chitin, and cellulose, whereas their antifungal activities were assayed using a direct confrontation method between tested bacterial isolates and pathogenic fungal on Malt Extract Agar medium. Molecular identification of bacteria with antifungal activity was performed by analyzing the 16S rRNA gene sequences. Isolation process of bacteria from monkey cup fluid resulted in 99 bacterial isolates with the ability to produce either protease, chitinase, and/or cellulose enzymes. A total of 37 bacterial isolates were capable of producing at least two hydrolytic enzymes. Antifungal assay of those bacteria showed that as many as 25 isolates have the ability to inhibit the growth of Fusarium sp. Based on the analysis of 16S rRNA gene sequences revealed that those isolates were closely related to three Burkholderia species, namely B. arboris, B. contaminans, and B. rijonensis.
Keywords: antifungal, Burkholderia, chitinase, cellulaseN, epenthes, protease
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Hardianti, Alfi Rizca, Helga Lusiana, and Dita Widayanti Sawitri. "Growth Response Test on Potential Indigenous Bacteria to Degrade Naphthalene and Phenanthrene on various pH." Bioscience 3, no. 2 (October 31, 2019): 121. http://dx.doi.org/10.24036/0201932105264-0-00.
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This study aims to understand the growth response of indigenous bacteria originating from oil sludge in Dumai which has the potential as Naphthalene and Fenantren degrading agents based on variations in substrate pH. The isolates used in this study were Isolate A, Isolate E, and Isolate F as a result of isolation from oil sludge in Dumai. Based on the results of the growth response test on various concentration in previous studies, we chose E isolates to be tested for its degradation ability. The bacterial growth response data was obtained based on OD measurements every 24 hours using a spectrophotometer and TPC at 72 hours using Nutrient Agar media. Based on these data, we determined the optimum pH for indigenous bacterial growth potential to degrade naphthalene or phenanthrene. In all variations of pH, bacterial isolate E experienced growth. This shows that bacterium E can use naphthalene and fenantren at normal pH range (pH 5-9).
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Jamaludin, Siani La, Johanis Fritzgal Rehena, Cecilia Anna Seumahu, and Dominggus Rumahlatu. "Isolation and Identification of Protease Enzyme Producing Bacteria from Fermentation of Gonad Sea Urchin (Echinothrix calamaris)." ILMU KELAUTAN: Indonesian Journal of Marine Sciences 23, no. 4 (January 4, 2019): 187. http://dx.doi.org/10.14710/ik.ijms.23.4.187-198.
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Bekasang of gonad sea urchin is one of the traditional fermentation products which generally involves microorganism spontaneous fermentation. Fermented paste products have a long shelf life and are processed quite easily using protease enzymes. Good exploration of producing protease from bakasang is needed to obtain the protease enzyme-producing microorganism with different characters. The method used in this research is screening with clear zone, measuring the activity of crude extract of protease enzyme characterization of bacteria through gram staining. Identification of potential microorganisms through 16S rRNA sequence. The results showed that there were eight isolates of protease enzyme-producing bacteria (G1, G2, G3, G4, G5, G6, G7, and G8) indicated by clear zones around single-colonic bacterial streaks. Only five bacterial isolates (G1, G4, G6, G7, and G8) were tested for the enzyme activity. These isolates have characteristics of positive gram bacteria. The interpretation of the results of molecular analysis using PCR and BLASTN sequences of 16S rRNA gene from five bacterial isolates, showed the identity of bacteria as: G1 was Staphylococcus piscifermentans strain CIP103958 with 99% similarity; Isolate G4 was Staphylococcus saprophyticus strain ATCC 15305 with 99% similarity; Isolate G6 was Staphylococcus condimenti F-2 strain with 99% similarity; Isolate G7 was Bacillus amyloliquefaciens subsp. plantarum strain FZB42 with 99% similarity; And G8 isolates was Lactobacillus plantarum strain JCM 1149 with 99% similarity.
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Kurniawan, Sembodho Edi, Mahyarudin Mahyarudin, and Ambar Rialita. "Aktivitas antibakteri isolat bakteri endofit daun pegagan (Centella asiatica) terhadap Staphylococcus aureus." Bioma : Jurnal Ilmiah Biologi 10, no. 1 (March 9, 2021): 14–29. http://dx.doi.org/10.26877/bioma.v10i1.7140.
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ABSTRAKAncaman global pada kasus Methicillin Resistant Staphylococcus aureus (MRSA) membutuhkan alternatif penanganan dengan tanaman obat tradisional. Bakteri endofit pada tanaman pegagan (Centella asiatica) memiliki kemampuan menghasilkan senyawa metabolit sekunder bersifat antibakteri yang serupa dengan tanaman inangnya. Tujuan penelitian ini untuk mengetahui aktivitas antibakteri isolat bakteri endofit daun pegagan (C. asiatica) terhadap S. aureus. Penelitian ini bersifat deskriptif yaitu isolat bakteri endofit daun pegagan (C. asiatica) diujikan dengan metode difusi cakram terhadap S. aureus. Isolat yang paling berpotensi memiliki aktivitas antibakteri dilakukan uji metabolit untuk mengetahui senyawa antibakteri yang dihasilkan. Identifikasi bakteri endofit berdasarkan morfologi koloni, morfologi sel, dan uji biokimia. Hasil penelitian menunjukkan 2 dari 37 isolat memiliki aktivitas terhadap S. aureus dengan zona hambat sebesar 9,02 mm dan 15,9 mm. Isolat yang paling berpotensi memiliki aktivitas tertinggi yaitu isolat I2 dengan zona hambat sebesar 15,9 mm. Isolat I2 memiliki kemiripan dengan genus Bacillus dan kemampuan mengasilkan senyawa antibakteri seperti alkaloid, saponin dan terpenoid. Kata kunci: antibakteri; bakteri endofit; Centella asiatica; Staphylococcus aureus ABSTRACTAntibacterial activity of endophytic bacteria isolate from pegagan leaves (Centella asiatica) against Staphylococcus aureusThe global threat in the case of Methicillin Resistant Staphylococcus aureus (MRSA) requires alternative treatment using traditional medicinal plants. Endophytic bacteria found in Pegagan plants (Centella asiatica) have ability to produce secondary metabolites with antibacterial capabilities similar to their host plants. The purpose of this study is to determine the antibacterial activity of endophytic bacterial isolates of Pegagan (C. asiatica) against S. aureus. This study is a descriptive research where endophytic bacterial isolates of Pegagan leaves (C. asiatica) were tested with disk diffusion method against S. aureus. The most potential isolates with antibacterial activity were performed metabolites test to determine the antibacterial compounds produced. Endophytic bacteria identification based on colony morphology, cell morphology and biochemical tests. The results showed that 2 out of 37 isolates had activity against S. aureus with inhibition zone of 9,02 mm and 15.9 mm. The most potential isolate that has highest activity was I2 isolate with inhibition zone of 15.9 mm. Isolate I2 has similarities with the genus Bacillus and the ability to produce antibacterial compounds such as alkaloids, saponins and terpenoids. Key words: antibacterial; Centella asiatica; endophytic bacteria; Staphylococcus aureus
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Rahman, Md Shahinur, Quazi Forhad Quadir, Atiqur Rahman, Moonmoon Nahar Asha, and Md Abul Khair Chowdhury. "Screening and characterization of Phosphorus solubilizing Bacteria and their effect on Rice seedlings." Research in Agriculture Livestock and Fisheries 1, no. 1 (February 22, 2015): 27–35. http://dx.doi.org/10.3329/ralf.v1i1.22353.
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An experiment was carried out to isolate, screen and characterize bacteria collected from an industrially polluted site of Bhaluka under the Mymensingh district and to evaluate their phosphorus (P) solubilizing capacity. About ten plant and soil samples from six different spots were collected from the site. Thirty four bacterial isolates were screened and pure cultures of the different bacterial isolates were prepared. Among the bacterial isolates 25 were gram negative and 9 were gram positive. About 31 bacterial isolates had catalase producing capacity and remaining 3 were negative to catalase test. Bacterial isolates were grown on a NBRIP media to determine their phosphorus solubilizing capacity. About 25 bacterial isolates were shown P solubilizing capacity. Isolate SB8 gave the highest result about 11.42 PSI (phosphorus solubilizing index), whereas other bacterial isolates showed moderate P solubilizing capacity (PSI 1.75-6.35). A plant trial with selected isolates (SB8, SB15, SB25) were also done and SB8 achieved 10% higher P content in comparison with control which supports the in vitro P solubilization assays. DOI: http://dx.doi.org/10.3329/ralf.v1i1.22353 Res. Agric., Livest. Fish.1(1): 27-35, Dec 2014