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Dissertations / Theses on the topic 'Bacterial isolation'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Kshetry, Nina. "Isolation and characterization of bacterial polyhydroxybutyrate inclusions." Thesis, Massachusetts Institute of Technology, 2006. http://hdl.handle.net/1721.1/35690.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, 2006.<br>Includes bibliographical references (leaves 79-83).<br>Polyhydroxybutyrate (PHB) is a carbon reserve found in some bacteria, and under nutrient limiting conditions accumulates intracellularly in the form of inclusion bodies. These inclusions contain proteins, and the PHB within the inclusions exists in an amorphous state. In this study a procedure to recover native PHB inclusions was developed, and the isolated inclusions were characterized using 13C NMR, western blotting, SDS-PAGE, ato

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2

Silva, Avalos Juan G. (Juan Guillermo). "Isolation, Characterization and Physiological Studies of Cyanide-Utilizing Bacteria." Thesis, University of North Texas, 1991. https://digital.library.unt.edu/ark:/67531/metadc278291/.

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Ten bacteria capable of growth on the metal-cyano complex, tetracyanonickelate (II) {K2 [Ni(CN)J } (TCN), supplied as the sole nitrogen source, were isolated. Seven isolates were identified as pseudomonads while the remaining three were classified as Klebsiella species. In addition to TCN, all isolates were able to utilize KCN although it was significantly more toxic. The degradation of TCN was most complete when supplied at growth-limiting concentrations, did not occur when ammonia was present, and resulted in the formation of nickel cyanide [Ni(CN)2] as a degradation product.

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3

Ross, Carolyn Felicity. "Screening, isolation and characterization of bacterial beta-galactosidases." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ27539.pdf.

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4

Bresler, Matthew Michael. "Isolation, cloning and characterisation of a bacterial cocaine esterase." Thesis, University of Cambridge, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624930.

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5

Whitaker, Sarah Louise. "Isolation of lux genes for biopollutant monitoring." Thesis, University of Nottingham, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310838.

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6

Wood, Helen Louise. "Isolation of novel bacterial antigens associated with ventriculoperitoneal shunt infections." Thesis, University of Nottingham, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342498.

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7

MINNECI, MARCO. "DETECTION AND ISOLATION OF THE BACTERIAL SECOND MESSENGER (P)PPGPP." Doctoral thesis, Università degli Studi di Milano, 2021. http://hdl.handle.net/2434/840186.

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This work has been carried out within the framework of the ERC funded project ERACHRON (Eradicating chronic infection), directed to the study of the chemical biology of bacterial persisters. Persisters are dormant phenotypic variants that are present in small amount in microbial populations. This phenotype shows high tolerance to antibiotics and for this reason it plays a key role in chronic infections. Indeed, after antibiotic treatment kills most of the bacterial population, persisters surviving the treatment can revert to the active phenotype, resuming growth and restoring the original, ant

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8

Newbould, E. C. "Catabolism of naphthalene sulphonic acids by three strains of bacteria." Thesis, University of Kent, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379697.

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9

Daoud, N. N. "Isolation and characterization of antifungal antibiotics synthesized by Myxococcus spp." Thesis, University of Salford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376848.

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10

Patten, Cheryl L. "Isolation and characterization of a bacterial auxin gene and its promoter." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/NQ65255.pdf.

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11

Vaithanomsat, Pilanee. "Isolation and analysis of recombinant EPSP synthases from microbial pathogens and cyanobacteria." Thesis, Imperial College London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325387.

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12

Thurgood, Trever Leon. "Isolation, Genetic Characterization and Clinical Application of Bacteriophages of Pathogenic Bacterial Species." BYU ScholarsArchive, 2019. https://scholarsarchive.byu.edu/etd/8586.

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Bacteriophages (phages) are the smallest biological entity on the planet. They provide vast amounts of valuable knowledge to biologists. Phage genomes are relatively simple compared to the organisms they infect (prokaryotes) and yet continually point to the complexity surrounding molecular- and microbiological mechanisms of life. By studying phages we can learn of the systems of gene expression, protein interaction and DNA organization. Phages are useful not only from an academic perspective, but may also have useful clinical applications. In the face of the rise of antibiotic-resistant bacter

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13

Klein, Timothy Matsiko Ninsiima. "The isolation and characterisation of novel natural products from marine bacterial symbionts." University of the Western Cape, 2015. http://hdl.handle.net/11394/4747.

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>Magister Scientiae - MSc<br>Drug-resistant infections are a global health crisis and drastically hinder the treatment options to effectively combat disease. Today, natural products remain an important source of novel drug candidates. Micro-organisms, in addition to being a source of bioactive natural products, represent a sustainable source of these compounds. As the marine environment is largely underexplored, the oceans represent a potential source of novel NPs. This study aimed at the discovery of novel NPs from bacteria associated with novel marine invertebrate species endemic to the Sout

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14

Pal, Roy Moushree. "Isolation, characterization, molecular gene cloning and expression of novel bacterial phytase from environmental samples." Thesis, University of North Bengal, 2015. http://hdl.handle.net/123456789/2744.

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15

Sung, Youlboong. "Isolation and Ecology of Bacterial Populations Involved in Reductive Dechlorination of Chlorinated Solvents." Diss., Georgia Institute of Technology, 2005. http://hdl.handle.net/1853/11653.

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The findings of this study demonstrate that Dehalococcoides species are intimately involved in complete reductive detoxification of chlorinated ethenes and are widely distributed in anoxic sediments and aquifers, including non-contaminated (pristine) environments. Careful examination of enrichment culture dechlorination kinetics, 16S rRNA gene based analyses, and reductive dehalogenase gene targeted PCR approaches revealed that complete reductive dechlorination is carried out by multiple dechlorinators. Two new dechlorinating species were isolated from contaminated and non-contaminated site

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16

Johnson, Penelope A. "Isolation and characterization of Drosophila melanogaster genomic DNA sequences containing bacterial insertion sequences." Thesis, University of Ottawa (Canada), 1987. http://hdl.handle.net/10393/5227.

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17

Kelly, Bridie Angela. "Process development for the production of microbial enzymes (salicylate hydroxylase and leucine aminopeptidase)." Thesis, University of Ulster, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.268616.

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18

Pinyon, Rebecca A. "Isolation and characterisation of novel non-ribosomal peptide synthetase genes from the entomopathogenic Xenorhabdus bovienii T228." Title page, contents and abstract only, 2002. http://web4.library.adelaide.edu.au/theses/09PH/09php659.pdf.

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19

Angeh, Jeremiah Eloh. "Isolation and characterization of anti-bacterial compounds present in members of Combretum section, Hypocrateropsis." Thesis, University of Pretoria, 2006. http://hdl.handle.net/2263/28269.

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20

Leung, Ka-ming, and 梁家銘. "Isolation, identification and establishment of bacterial culture collection of fish pathogens in Hong Kong." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/207649.

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The importance of fish culture has been increasing since 1990’s. The steady growth of fish culture helps to ensure a stable supply of fish for human consumption. However, when compared with capture fisheries, production from fish culture is greatly influenced by fish diseases. Outbreaks of fish diseases have caused great economic loss to fish culture. Research has been conducted to understand and reduce the occurrence of fish diseases in fish culture. In Hong Kong, bacterial infection is the most common cause of fish diseases. This project is therefore directed to isolate and identify the caus

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21

Khan, Mirzaei Mohammadali. "The efficacy of bacterial viruses against multi-resistant Escherichia coli: from isolation to pharmacology." Doctoral thesis, Stockholms universitet, Institutionen för molekylär biovetenskap, Wenner-Grens institut, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-126328.

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The increase of multi-resistant bacteria highlights that the golden era of antibiotics is ending and that alternative treatmentsare urgently needed. Phages have been historically used to treat bacterial infections prior to the discovery of antibiotics and have gained renewed interest in the past decade. Despite the advantages of phage therapy over traditional antibiotic usage, a number of concerns persist over their clinical application centring on their efficacy and safety. This thesis presents four papers that focus on the isolation and characterization of phages that target reference strain

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22

Ta, Chieu Anh Kim. "Bacterial Biofilm Inhibition and Antifungal Activity of Neotropical Plants." Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/32419.

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This thesis examined the antimicrobial activity of select neotropical plants from Costa Rica and traditional Q’eqchi Maya medicines from Belize. In particular the potential for interference with bacterial quorum sensing (QS) and biofilm formation as well as fungal growth were assessed. Of one hundred and twenty six extracts collected from Costa Rica, one third showed significant QS inhibition while 13 species displayed more biofilm inhibitory activities than the positive control allicin. The active species belonged to the Lepidobotryaceae, Melastomataceae, Meliaceae, Sapindaceae, and Simarouba

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23

Barratt, Emma Elizabeth. "The isolation, purification and characterisation of an alkaline alpha-galactosidase from a thermophilic bacterial consortium." Thesis, Cranfield University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341047.

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24

Nordstedt, Nathan P. "Isolation and characterization of novel bacterial strains to alleviate abiotic stress in greenhouse ornamental crops." The Ohio State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1617740819791342.

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25

Thompson, Biosha. "Isolation and characterization of bacterial endophytes for growth promotion of Phaseolus vulgaris under salinity stress." University of the Western Cape, 2020. http://hdl.handle.net/11394/8078.

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>Magister Scientiae - MSc<br>As the global human population grows, so does the demand for faster food production rates. Owing to this, agricultural practices have had to expand and move into semi-arid and arid regions, too, where frequent irrigation is essential. However, irrigated ground water contains many salt ions (mainly Na+ and Cl-) which contribute to soil salinization on croplands. Soil salinity negatively impacts crop growth and yield and thus, strategies for the alleviation of salt stress on crop plants have had to be developed. This study assessed the use of plant growth promoting b

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26

Mathopa, Byatlela Abel. "Isolation of bacterial strains capable of efficient conversion of n-alkanes into value added products." Master's thesis, University of Cape Town, 2004. http://hdl.handle.net/11427/4294.

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Includes bibliographical references.<br>Bacteria that are capable of degrading alkane fractions, C12-C13 and C14-C17, were isolated from oil-contaminated soil collected from the petrochemical company, CALTEX Refineries, South Africa. A total of twenty-three environmental strains were isolated. A preliminary procedure, Nile Blue A straining suggested that twelve of the twenty-three environmental strains might accumulate polyhydroxyalkanoates (PHAs) in their cytoplasm, which is a good candidate for biodegradable plastics. The gene that catalyzes PHA polymerization, phaC, was detected using PCR i

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27

Toth, Ian K. "The isolation of novel Erwinia phages and their use in the study of bacterial phytopathogenicity." Thesis, University of Warwick, 1991. http://wrap.warwick.ac.uk/109204/.

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A number of bacteriophages were isolated on the "soft rot" phytopathogens Erwinia carotovora subsp. atroseptica SCRI1043 and Erwinia carotovora subsp. carotovora SCRI193. Several of these phages were used to obtain phage resistant mutants of SCRI1043, in order to investigate the role of the bacterial cell surface in virulence. While a number of phenotypic properties relating to pathogenicity and virulence of this strain have already been uncovered, little is known about the role of the cell surface in virulence. It was hoped that the use of phages would allow selection of mutants altered in bo

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28

Wang, Yanling, and 王{227b76}[ling]. "Isolation and characterization of environmental vibrio species from Mai Po Nature Reserve, Hong Kong." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B29232028.

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29

Rajab, Mais Mahmoud Sheikh. "Isolation and characterisation of a bacterial strain with control activity against post-harvest mould from citrus." Thesis, Heriot-Watt University, 2016. http://hdl.handle.net/10399/3285.

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The susceptibility of citrus fruits to microbial pathogens, especially green and blue moulds, is one of the difficulties that face citrus fruits suppliers to keep these fruits in good condition for consumers. Therefore, there is an urgent need for a safe and efficient method to preserve them during transportation and storage. This study focused on searching for a bacterial strain that have antifungal activity against moulds that attack citrus fruits. Environmental samples were screened for microorganisms with antifungal activity against Penicillium fungi that were isolated from infected lemon

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30

Siebritz, Alex. "Isolation and characterization of stem endophytic bacteria from weed plants for enhancing Vanadium tolerance in Brassica napus." University of the Western Cape, 2019. http://hdl.handle.net/11394/7226.

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Masters of Science<br>Bacterial endophytes are able to improve the growth of their hosts through a number of different mechanisms such as nutrient uptake regulation, plant hormone production and regulation, siderophore production and phosphate solubilisation. They have also been shown to be able to provide protection to plants against various abiotic stressors, through various means such as oxidative stress protection. The purpose of this study was therefore to isolate endophytic bacteria from the stems of different weeds, to characterize their ability to use some of the most important g

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31

Krallis, Myrsini. "Isolation and identification of Beta-Lactam Producing Microorganisms using PCR based methodologies." Thesis, Rhodes University, 1997. http://hdl.handle.net/10962/d1018237.

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The polymerase chain reaction (PCR) was investigated as a potential tool in microbial screening for 13-lactam. producing organisms. Optimization of PCR conditions and the addition of acetamide to the PCR reaction allowed for the successful amplification of the isopenicillin N synthetase (lPNS) gene in S. clavuligerus, S. tanashiensis, S. griseus, S. olivaceus, S. lipmanii, and S. chartreusis. PCR was used to produce a radiolabelled probe from S. clavuligerus that was used to detect analogous genes in bacteria and fungi. Southern blot and dot blot analysis using the lPNS probe revealed the pres

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32

Bagla, Victor Patrick. "Isolation and characterization of compounds from Podocarpus henkelii (Podocarpaceae) with activity against bacterial, fungal and viral pathogens." Thesis, University of Pretoria, 2012. http://hdl.handle.net/2263/24465.

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Diseases caused by bacteria, fungi and viruses pose a significant threat especially to poor rural communities. Viral infections are frequently complicated by secondary bacterial and fungal infections which remain a major challenge globally and in particular, in sub Sahara Africa amongst humans and animals alike. The main aim of this study was to develop a low toxicity plant extract or isolated compound active against viral, bacteria and fungal pathogens from selected plant species. Seven tree species that were investigated were Acokanthera schimperi, Carissa edulis, Ekebergia capensis, Podocar

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33

Mulder, Michelle Anne. "The Mycobacterium tuberculosis KatG gene : identification of a novel function and analysis of the regulation of expression." Doctoral thesis, University of Cape Town, 1998. http://hdl.handle.net/11427/25662.

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A clone containing the terminal third of the Mycobacterium tuberculosis katG gene was previously shown to confer resistance to ethyl methane sulfonate on DNA repair-deficient Escherichia coli cells. The first aim of this study, therefore, was to examine the role played by the M tuberculosis katG gene in DNA repair. The strategy used was overexpression of different regions of the gene in DNA repair-deficient mutants of E. coli, and examination of the sensitivities of the transformants to DNA damaging agents. Overexpression of the gene resulted in an increase in the survival of recA mutants expo

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34

Vadari, Yoganand. "Estimation of Microbial Diversity in Poultry Litter Using Terminal Restriction Fragment Length Polymorphism and Isolation of Phosphate Accumulating Bacteria from Poultry Litter." TopSCHOLAR®, 2004. http://digitalcommons.wku.edu/theses/239.

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The contamination of fresh water by phosphates in poultry litter results in substantial eutrophication of fresh water causing fish kills and other types of environmental damage. The poultry indus try in Kentucky is expanding rapidly. The number of broilers is increasing as more poultry farms are established in the state producing waste that needs disposal. Investigations were made to study the possibility of using microorganisms normally found in poultry litter to sequester phosphate, thereby delaying phosphate runoff after litter is applied to croplands. Little is known, however, about the mi

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35

Kreutzer, Martin Florian [Verfasser], Markus [Akademischer Betreuer] Nett, Dirk [Akademischer Betreuer] Hoffmeister, and Gabriele M. [Akademischer Betreuer] König. "Isolation, characterization and structure elucidation of bacterial siderophores / Martin Florian Kreutzer. Gutachter: Markus Nett ; Dirk Hoffmeister ; Gabriele M. König." Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2014. http://d-nb.info/1049437748/34.

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36

Rode, Alexander. "Isolierung und Charakterisierung von bakteriellen extrazellulären polymeren Substanzen aus Biofilmen / Isolation and characterization of bacterial extracellular polymeric substances from biofilms." Gerhard-Mercator-Universitaet Duisburg, 2004. http://www.ub.uni-duisburg.de/ETD-db/theses/available/duett-09132004-102114/.

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Microorganisms in biofilms are kept together by extracellular polymeric substances (EPS). The EPS are key molecules for the structure, function and organization of biofilms. Chemical and / or physical isolation methods are being used for the quantitative separation of EPS from biofilms. The yield of EPS depends on the method of isolation. Four different methods of EPS isolation were used in this work (separation by stirring and centrifugation, use of a cation exchange resin, extraction with formaldehyde and extraction with formaldehyde and NaOH) on pure culture biofilms of Pseudomonas aerugino

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37

Daggas, Traianos. "Isolation and screening of bacterial biocontrol agents and their use for disease control of tomato and cucumber greenhouse-grown crops." Thesis, University of Aberdeen, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249788.

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38

Briehl, Margaret Marie. "Isolation of a set of mutations linked to the TAG-1 locus of Bacillus subtilis, which perturb cell surface properties." Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184343.

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The physiological role of the teichoic acid polymers found in Gram-positive bacterial cell walls is not known. Studies of Bacillus subtilis hybrid strains implicate a defined chromosomal region, which includes the tag-1 locus, as necessary for teichoic acid biosynthesis. A set of ten mutants carrying lesions in this region was identified from among forty-four temperature-sensitive (ts) mutants generated by nitrosoguanidine mutagenesis and bacteriophage 029 selection. This protocol gave a population enriched for ts, versus auxotrophic, mutants. For each of the ten mutants, the frequency of gene

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39

Zhao, Bingyu. "Isolation and characterization of the maize nonhost resistance gene Rxo1 and the corresponding bacterial effector gene avrRxo1 from Xanthomonas oryzae pv. oryzicola /." Search for this dissertation online, 2004. http://wwwlib.umi.com/cr/ksu/main.

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40

Sakong, B. M. (Bellonah Motshene). "Isolation and characterization of compounds from Calodendrum capense (Rutaceae) and Lydenburgia cassinoides (Celastraceae) for treatment of fungal and bacterial infections in immunocompromised patients." Diss., University of Pretoria, 2012. http://hdl.handle.net/2263/25170.

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Infectious diseases are a serious concern worldwide especially in immune-compromised patients. Some of these diseases are considered to be contagious and are spread via airborne transmission, while others are not contagious, i.e. non-communicable diseases. The problem is compounded by the emergence of pathogens resistant to currently used antimicrobial drugs. A wide range of microbes including bacteria, fungi, parasites, viruses and protozoans are implicated as causative agents of various diseases. Many patients without ready access to Western medical facilities rely on medicinal plants for th

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41

Schroeder, Declan Cosmo. "Isolation and characterization of a β(1-4) agarase of an epiphytic bacterial pathogen, Pseudoalteromonas gracilis B9, of the red alga, Gracilaria gracilis". Doctoral thesis, University of Cape Town, 2001. http://hdl.handle.net/11427/4328.

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42

Mandal, Hrisikesh. "Isolation and characterization of Ralstonia solanacearum (Smith) Yabuuchi et. al. causing bacterial wilt of tomato from sub-Himalayan West Bengal and its management." Thesis, University of North Bengal, 2021. http://ir.nbu.ac.in/handle/123456789/4800.

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43

Horton, Steven Andrew. "Microarray Analysis of Streptococcus mutans and Actinomyces viscosus in Homologous and Heterologous Cultures." Scholar Commons, 2008. https://scholarcommons.usf.edu/etd/300.

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The oral pathogen Streptococcus mutans is a known etiological agent for dental root decay and coronal caries. It has been hypothesized, but not yet proven, that S. mutans expression of virulence genes in dental plaque may be influenced by its interaction with co-aggregating partners, notably Fusobacterium nucleatum and Actinomyces viscosus. Investigation of the suitability of mixed cultures of S. mutans with F. nucleatum versus S. mutans with A. viscosus proved that A. viscosus was a better target in the present laboratory setting. Furthermore, A. viscosus, a causative agent of mandible osteom

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44

Thongmee, Acharawan. "Isolation and Characterization of a New Capsule-Forming Bacterium." Thesis, University of North Texas, 1999. https://digital.library.unt.edu/ark:/67531/metadc500460/.

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A unique, previously undescribed Gram-negative bacterium was isolated from several soils in Texas and extensively characterized in this study. The cells measured 1-2 by 4-6 μm. The distinguishing characteristic of the bacterium is the extraordinary capsular material which surrounds the cells. The new isolates are aerobic, mesophilic, non motile and have the ability to utilize a variety of organic compounds as the sole source of carbon and energy. The organism grows optimally at 30° C and the optimal pH lies between 7.0-8.0. The isolates produce catalase but oxidase is not produced. They do not

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Agnol, Alais Maria Dall. "Comparação entre isolamento bacteriano e PCR de Streptococcus suis tipo 2 detectados em tonsilas de suínos de abate em Santa Catarina." Universidade do Estado de Santa Catarina, 2014. http://tede.udesc.br/handle/handle/904.

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Made available in DSpace on 2016-12-08T16:24:16Z (GMT). No. of bitstreams: 1 PGCA14MA129.pdf: 560924 bytes, checksum: 728efdf249e90f0bd6744c133006c9a1 (MD5) Previous issue date: 2014-02-26<br>Streptococcus suis is a important pathogen associated to many production and economical losses in the swine industry, in addition to its zoonotic importance. The aims of this study was evaluate the occurrence of Streptococcus suis type 2 in tonsil samples of healthy pigs at slaughter, comparing the bacterial isolation with PCR assay and to determine the presence of the EF factor (extracellular protein f

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46

Hrudíková, Radka. "Využití magnetických mikročástic pro izolaci bakteriální DNA." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2012. http://www.nusl.cz/ntk/nusl-216876.

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The aim of the work was testing of two types of magnetic mikrosheres functionalised with –COOH groups for the isolation of bacterial DNA. Isolation of DNA was carried out from crude lysates of cells prepared from pure culture of Lactobacillus paracassei RL-10 in the presence of binding buffer with 2 M NaCl and 16% PEG 6000. The influence of RNA degradation by enzyme RNase A on the amount of isolated DNA was investigated. It was estimated that RNA degradation affects the amount of DNA isolated. The amount of DNA depended on the type of microparticles. Higher amounts of DNA were isolated using p

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47

Oestreicher, Zachery Walter John. "Magnetotactic Bacteria: Isolation, Imaging, and Biomineralization." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1354146141.

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48

Domingues, Patrícia Maia. "Isolation of estuarine biosurfactant-producing bacteria." Master's thesis, Universidade de Aveiro, 2011. http://hdl.handle.net/10773/7773.

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Mestrado em Biotecnologia<br>Bioremediation has proven to be an effective strategy in the recuperation of oil contaminated ecosystems. However most bacteria used in this processes, while being able to degrade a wide range of the oil hydrocarbons, have limited action due to the low water solubility of these compounds. Hence, a possible solution for this problem would be the use of biosurfactant-producing bacteria, since the presence of surfactants help improve the hydrocarbons dispersal, solubilization and bioavailability. The objective of this work was to assess the biotechnological pot

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49

KUTZ, SUSAN MARIE. "ISOLATION AND CHARACTERIZATION OF A HELICALLY TWISTED BACTERIUM RESEMBLING SELIBERIA." Diss., The University of Arizona, 1987. http://hdl.handle.net/10150/184075.

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A seliberia-like bacterium (SLO), isolated from reverse osmosis membranes was characterized by morphological, physiological and DNA studies. The helically twisted cells of this organism were often observed in star-shaped clusters. Depending on nutritional conditions, cells ranged from 0.5 to 21 um in length and possessed prosthecae. Small motile cells were produced by asymmetric fission or by a budding process. Ovoid "generative" cells were observed in mixed culture conditions or when the pure culture isolate was grown in the presence of humic acid. The SLO oxidatively utilized glucose, maltos

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50

Machan, Radoslav. "Izolace DNA s použitím nově syntetizovaných magnetických nosičů." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2018. http://www.nusl.cz/ntk/nusl-376843.

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Theoretical part of the master thesis was aimed on giving an overview of basic characteristics of magnetic particles, their morphology, basic methods of their synthesis, interaction with DNA and recent applications in biotechnology and biomedicine. The experimental part of thesis was aimed on application of new designed magnetic particles for isolation of both lactobacilli DNA and calf thymus DNA. Two types of magnetic beads were used: hyperbranched poly(glycidyl methacrylate-co-[2-(methacryloyloxy) ethoxy]acetic acid-co-ethylene dimethylacrylate) microbeads covered with amino groups (P(GMA-MO

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