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Journal articles on the topic 'Bacterial isolation'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Muwafaq Azeez, Iman, and Mohammed Nather Maa'roof. "Isolation and Identification of some Bacterial Species From Cervix Infected Women in Salahuddin Governorate." Tikrit Journal of Pharmaceutical Sciences 13, no. 1 (2023): 44–53. http://dx.doi.org/10.25130/tjphs.2018.13.1.7.44.53.

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This study was carried out for isolating some pathogenic types of positive and negative bacteria for Gram stain, which cause the infection of the vaginitis in infected women and its diagnosis by routine laboratory methods and confirm the diagnosis using Vitek2compact system by collecting fifty samples of the swab of the cervix in the period between October 2015 till April 2016 from the in patients and out patients women in Salahuddin Hospital for the age groups between (14-50) years.The results of isolation show the percentage of bacterial growth was 78%, it reached samples in 78%.The results

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Ayal, Esther Lourence Brendha, Kasprijo Kasprijo, Ren Fitriadi, et al. "Screening of Amylolytic Bacteria from Mina Padi Aquaculture in Panembangan Village, Cilongok District, Banyumas, Central Java." Journal of Aquaculture and Fish Health 13, no. 1 (2024): 90–101. http://dx.doi.org/10.20473/jafh.v13i1.39210.

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Amylolytic bacteria play an important role in the ecosystem, especially as probiotic and bioremediation agents in cultivation, as examples can be found in Mina Padi culture. The purpose of this study was to determine the amylolytic bacteria in the waters of the Mina Padi pond. Bacterial isolation began with bacterial sampling, inoculation and isolation of bacteria, calculation of the total abundance of bacteria, observation of bacterial morphology and bacterial purification, and isolation of amylolytic bacteria. The results of the isolation of amylolytic bacteria obtained 3 bacterial isolates

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Tabassum, Sana, S. Rajeswar Rao, G. Samatha Rani, L. Jaya Lakshmi, and G. Sasikala. "Isolation of Anaerobes in Bacterial Vaginosis." Scholars Journal of Applied Medical Sciences 4, no. 6 (2016): 2059–61. http://dx.doi.org/10.21276/sjams.2016.4.6.39.

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4

Ares, M. "Bacterial RNA Isolation." Cold Spring Harbor Protocols 2012, no. 9 (2012): pdb.prot071068. http://dx.doi.org/10.1101/pdb.prot071068.

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Rori, Chindy Achika, Febby Ester Fany Kandou, and Agustina Monalisa Tangapo. "Isolation and Antibacterial Test of Endophytic Bacteria of Avicennia marina Mangroves." Koli Journal : English Language Education 1, no. 1 (2020): 1–7. http://dx.doi.org/10.30598/koli.1.1.1-7.

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Endophytic bacteria are a source of extracellular compounds such as antimicrobial compounds. Endophytic bacteria and host plants have a symbiotic relationship of mutualism that allows bacteria to produce the same bioactive compounds as those contained in the host plant. This study aims to isolate and test the antibacterial potential of endophytic bacterial Avicennia marina mangroves. The research method used is experimental explorative, isolating bacteria from A. marina mangrove plants and then testing the potential of extracellular enzyme activity from endophytic bacterial isolates. The resul

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Khairani, Khairani, and Kartika Manalu. "Isolasi dan Identifikasi Bakteri Lipolitik dari Limbah Cair Kelapa Sawit (Elaeis quineensis Jacq.)." BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains 6, no. 1 (2023): 1–13. http://dx.doi.org/10.31539/bioedusains.v6i1.5285.

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This study aims to determine the ability of lipolytic bacteria to degrade lipids by obtaining isolates of lipolytic bacteria in palm oil industrial wastewater. By testing the ability of the lipolytic index, the research method used is a description method with bacterial isolation techniques using NA media (Nutrient Agar) and selective media for lipolytic agar. The results showed that there were 10 isolates of lipolytic bacteria with morphological and biochemical characteristics indicating three bacterial genera, namely, the genera Bacillus, Pseudomonas, and Klebsiella. Data analysis showed tha

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Johnson, Gayle C., William H. Fales, Carol W. Maddox, and Jose Antonio Ramos-Vara. "Evaluation of Laboratory Tests for Confirming the Diagnosis of Encephalitic Listeriosis in Ruminants." Journal of Veterinary Diagnostic Investigation 7, no. 2 (1995): 223–28. http://dx.doi.org/10.1177/104063879500700210.

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Retrospective analysis of 93 bovine, ovine, and caprine cases diagnosed as listerial encephalitis revealed positive bacterial isolations in only 63% of 59 cases in which bacterial culture was attempted. Only 42% of 41 attempted bovine brain cultures were successful, compared with 67% from 6 sheep brains and 92% from 12 goat brains. Gram stains and Listeria-specific immunohistochemistry were evaluated as tools for verifying the presence of bacteria or listerial antigens in 38 animals. Sixteen of 17 animals in this group with positive bacterial isolations were immunochemically positive for liste

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Lee, Yoo Kyung, Kae Kyoung Kwon, Kyeung Hee Cho, Jae Hyun Park, and Hong Kum Lee. "Isolation and Identification of Bacteria from Marine Biofilms." Key Engineering Materials 277-279 (January 2005): 612–17. http://dx.doi.org/10.4028/www.scientific.net/kem.277-279.612.

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In the marine environment, biofilms cover most of the subtidal and intertidal solid surfaces. Culturable bacteria forming marine biofilms were isolated on artificial substrate called acrylic coupons. The bacterial isolates were identified through a comparison of 16S rDNA sequences. A total of 115 strains were cultured and identified, 45 of which showed the same sequences with other strains. Therefore, 70 strains were finally identified. The bacterial isolates belonged to a–Proteobacteria (32 isolates), g–Proteobacteria (12 isolates), CFB group bacteria (4 isolates), high GC Gram-positive bacte

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Yang, Haoran. "Optimization of isolation and identification methods of antibiotic-producing bacteria from marine microorganisms." Molecular & Cellular Biomechanics 22, no. 2 (2025): 815. https://doi.org/10.62617/mcb815.

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Marine environments are being investigated to identify microscopic forms of life which could produce botanic antibiotics as there is an increasing demand for newer antibiotics which could be used to treat all bacteria due to the ever-increasing resistance of various forms of bacteria. In this study, we enhance the techniques for recovering and characterizing antibiotic-producing bacteria from seawater samples. Seawater samples were obtained from different sea areas, microorganisms were concentrated, and potential antibiotic-producing microorganisms were sought on selective media and in enrichm

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10

Ansam Khalid, Mohammed. "Comparative Study of Bacterial Contamination in Local Iraqi Sheep and Goats Semen." Iranian Journal of Veterinary Medicine 18, no. 1 (2024): 71–78. http://dx.doi.org/10.32598/ijvm.18.1.1005383.

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Background: Semen contamination is a detrimental factor in decreasing fertility. Seasonal changes may affect the contamination, too. Objectives: This study was designed to detect semen contamination in ovine and caprine during different seasons. Methods: Six fully mature male sheep and goats were subjected to electro-ejaculator collection twice monthly from February 1, 2022, to January 31, 2023 (Spring, February 1, 2022-April 30, 2022; Summer, May 1, 2022, July 31, 2022; Autumn August 1, 2022, October 31, 2022; Winter November 1, 2022, January 31, 2023), for studying the seasonal effect. A tot

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Sari, Desy Purnama, Hermansyah Amir, and Rina Elvia. "ISOLASI BAKTERI DARI TANAH TEMPAT PEMBANGAN AKHIR (TPA) AIR SEBAKUL SEBAGAI AGEN BIODEGRADASI LIMBAH PLASTIK POLYETHYLENE." Alotrop 4, no. 2 (2020): 98–106. http://dx.doi.org/10.33369/atp.v4i2.13833.

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This study aims to obtain the bacteria of Pseudomonas from the land of TPA Air Sebakul Bengkulu City as a plastic degrading agent and measure the ability of bacteria to degrade Low Density Polyethylene (LDPE) and Oxium plastic. The research was conducted from February to August 2019, at the Laboratory of Biology and Chemistry Learning, Faculty of Teacher Training and Education, University of Bengkulu. Air Sebakul landfill samples were taken at the coordinate point 3o49 '27.8 "S 102o20 '48.4" E. Isolation of plastic degrading bacteria using selective media King's B Agar added with 2% Polyethyle

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KHALED, ABDULLAH MOBARKI, IBRAHIM ALSHAMLAN FAISAL, MOHAMMED ALBUAYJAN ABDULELAH, Hussein Saleh Bani Hamim Khaled, and Saad Mohammed Alqahtani Mohammed. "Bacterial Culture in Clinical Microbiology." International Journal of Healthcare Sciences 11, no. 2 (2023): 95–100. https://doi.org/10.5281/zenodo.10435398.

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<strong>Abstract:</strong> Bacterial culture is a fundamental technique in clinical microbiology that involves the growth and isolation of bacteria from patient samples. This technique has evolved significantly over the years, with improvements in both the methods of isolation and identification of bacteria. In this essay, we will discuss the current and past strategies for bacterial culture in clinical microbiology. In the past, the primary method for bacterial culture involved plating patient samples onto solid growth media, such as blood agar or MacConkey agar, and incubating the plates at

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Rahmiati, Rahmiati, Sri Pujianto, and Endang Kusdiyantini. "Eksplorasi Mikroba Penghasil Enzim-enzim Hidrolitik Di Kawasan Taman Nasional Lore Lindu Sulawesi Tengah." Bioma : Berkala Ilmiah Biologi 18, no. 2 (2016): 14. http://dx.doi.org/10.14710/bioma.18.2.14-19.

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Lore Lindu National Park (TNLL) is an area that flora, fauna and microbes, the diversity of microbes producing hydrolytic enzyme. explore the hydrolytic enzyme producing microbes in Indonesia. This study was aimed to obtain bacterial isolates were able to produce hydrolytic enzyme and characteristics. Isolation in the microbiology laboratory. Isolation by a spread plate. Isolates in the selection hydrolytic enzyme producing selective media. Measurement of the activity of the enzyme with hydrolytic index. The results were thirteen isolates clearing zone test , 2 protease enzyme bacterial isolat

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Sillman, Carl E., and L. E. Casida Jr. "Isolation of nonobligate bacterial predators of bacteria from soil." Canadian Journal of Microbiology 32, no. 9 (1986): 760–62. http://dx.doi.org/10.1139/m86-139.

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15

Jwad Abid-Al-Khadim, Nisreen, and Aalaa Fahim Abbas. "Selective Isolation of Heavy metals Resistant Bacteria From Waste Water." Al-Qadisiyah Journal of Pure Science 25, no. 4 (2020): 1–12. http://dx.doi.org/10.29350/qjps.2020.25.4.1203.

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The current study was conducted to isolate bacteria resistant to heavy metals lead, chromium and cadmium from waste water. The concentration of lead, chromium and cadmium was measured in the waste water samples collected from different sites in Baghdad city using a flame atomic spectrometry. The results showed a significant increase in metals concentration in some sites compared to the standard determinants, as well as a significant variation between the concentration of the three metals, and nil values were recorded in some sites.The primary isolation of lead, chromium and cadmium tolerant ba

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Tania Salsabila, Ananda, Aninditia Sabdaningsih, and Haeruddin . "Morphological Characteristics of Methanotroph Bacteria Isolated from Sediment Samples in Ponds." International Journal of Advanced Multidisciplinary Research and Studies 4, no. 5 (2024): 275–78. http://dx.doi.org/10.62225/2583049x.2024.4.5.3228.

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Semarang is one of the largest cities in Indonesia, and it was a coastal city with 33 km of coastline in 2016. The high emission and waste in Semarang City affect water pollution which impacts the presence of microorganisms such as bacteria. Isolation of anaerobic bacteria has been explored due to the difficulties of handling. One type of anaerobic bacteria is methanotroph bacteria. The lack of literature on methanotroph bacteria in the aquatic environment which in this study is specific to ponds, it is necessary to explore. The study aimed to isolate and determine the characteristics of metha

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Fatimah, Fatimah, Annida Izzatul Millah, Risky Lailatul Ayu Fadilah, et al. "Isolation and Potency Test of Endophytic Bacteria as Nitrogen Fixer from Mangrove Plant in Lamongan." Jurnal Riset Biologi dan Aplikasinya 4, no. 1 (2022): 26–33. http://dx.doi.org/10.26740/jrba.v4n1.p26-33.

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Endophytic bacteria are microorganisms that live in plant tissues and some of them contribute to nitrogen fixation for plants. This study aimed to isolate and identify endophytic bacteria from mangroves of Kutang Beach, Lamongan, which potentially as nitrogen fixing bacteria. Bacterial Isolates were used as candidates for biofertilizers. Leaves samples were taken from 10 sampling points. Bacterial isolation was initiated by sterilizing the surface of the leaves sample and grinding it aseptically. Isolation was carried out with a pour plate method on Nutrient Agar medium. Screening for endophyt

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18

KALITA, MICHAŁ, MARTA PALUSIŃSKA-SZYSZ, ANNA TURSKA-SZEWCZUK, SYLWIA WDOWIAK-WRÓBEL, and TERESA URBANIK-SYPNIEWSKA. "Isolation of Cultivable Microorganisms from Polish Notes and Coins." Polish Journal of Microbiology 62, no. 3 (2013): 281–86. http://dx.doi.org/10.33073/pjm-2013-036.

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The potential role of currency in the spread of pathogenic microflora has been evaluated in many countries. In this study Polish paper notes and the coins in general circulation were assayed for the presence of cultivable bacteria and fungi. Bacterial isolates identification was based on cultural and biochemical characters and by comparison of the 16S rRNA gene sequence. Fungal isolates were recognized with biochemical and morphological criteria. Coagulase-negative staphylococci, (43.6% of the total bacterial count) including Staphylococcus saprophyticus, S. epidermidis, and S. hominis, and En

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Schirmer, Abigail, Renard Sessions, Nikolaus Gravenstein, et al. "Isolation gowns as a potential work hazard." Annals of Work Exposures and Health 64, no. 6 (2020): 596–603. http://dx.doi.org/10.1093/annweh/wxaa047.

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Abstract Objectives Isolation gowns are used as a barrier to bacterial transmission from patient to provider and vice versa. If an isolation gown is ineffective, the patient and provider have a potential breach of safety and increased infection risk. This study compared the bacterial permeability of differently rated, commonly uses isolation gowns to assess their effectiveness in preventing simulated bacterial transmittance, and thus contamination, from patient to provider. Methods Serial dilutions of Staphylococcus epidermidis in sterile saline were applied to a simulated skin surface. Unrate

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Sitotaw, Baye, Fikremariam Ayalew, Abayneh Girma, et al. "Isolation and identification of promising antibiotic-producing bacteria." Open Chemistry 20, no. 1 (2022): 1283–91. http://dx.doi.org/10.1515/chem-2022-0233.

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Abstract Multiple stresses in waste dumpsite soils can drive antibiotic production as one of the strategies for survival. Bacteria are the most prolific producers of antibiotics. This study investigated the antibiotic production potential of bacteria isolated from Bahir Dar city municipal solid waste dumpsite (MSWDS). Bacteria were isolated from soil collected from the dumpsite on starch casein or nutrient agar. The isolates were carefully screened for antimicrobial activity against six pathogenic bacterial test strains. Minimum inhibitory concentration (MIC) and minimum bactericidal concentra

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Goncagul, Gulsen. "Antimicrobialsusceptibilityof bacteria isolated from goats with subclinical mastitis in the Southern Marmara region of Turkey." Medycyna Weterynaryjna 77, no. 05 (2021): 6527–2021. http://dx.doi.org/10.21521/mw.6527.

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The mastitis of dairy goats is a disease of the economic importance worldwide and is mostly associated with bacterial infections. The aim of this study was to isolate the bacteriae causing subclinical mastitis, and determine to the susceptibility of some clinical isolates against several antimicrobial agents frequently used to control bacterial subclinical mastitis in dairy goats in the Southern Marmara Region. A total of 68 Saanen goats were used for this investigation and subclinical mastitis was determined by using California Mastitis Test. As a result of bacteriological analysis of milk sa

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Safaa Zakara Baker, Qanat Mahmood Atiyae, and Marwa Salam Khairallah. "Isolation and Identification of some Species of Bacterial Pathogens from Musca Domestic and Test their Susceptibility Againts Antibiotics." Tikrit Journal of Pure Science 23, no. 9 (2023): 20–27. http://dx.doi.org/10.25130/tjps.v23i9.808.

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The current study was conducted during the period from the beginningof October 2016 to April 2017 to isolate and diagnose the bacteria on theouter surface of the domestic fly (Musca domestica) and to determine thesensitivity of bacterial isolates against antibiotics using differentagricultural mediums. Insects were collected from three sites in the Tikritcity which are: vegetable market, butchers market and sheep sheds. 50samples were collected from each site. Bacterial isolates obtained fromthe external surface of insects were subjected to morphological andbiochemical tests and API 20 E syste

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Maritsa, Hasna Ul, and Raudatul Jannah. "Isolation and identification of pathogenic bacteria in the Batanghari River, Jambi." BUMI: International Journal of Environmental Reviews 1, no. 01 (2023): 36–41. http://dx.doi.org/10.30631/bumi.v1i01.1903.

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Research on the isolation and identification of pathogenic bacteria in the Batanghari River, Jambi, was conducted from November to February 2023. The purpose of this research was to identify pathogenic bacteria in the Batanghari River, Jambi. The research method involved purposive sampling in the field and bacterial identification tests in the laboratory. Samples from the field were diluted, inoculated, and incubated on Nutrient Agar (NA) media for 24 hours. Macroscopic observations included the shape, color, elevation, texture, and surface of the bacterial isolates. Microscopic observations i

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Maritsa, Hasna Ul, and Raudatul Jannah. "Isolation and identification of pathogenic bacteria in the Batanghari River, Jambi." BUMI: International Journal of Environmental Reviews 1, no. 01 (2023): 36–41. http://dx.doi.org/10.30631/101.36-41.

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Research on the isolation and identification of pathogenic bacteria in the Batanghari River, Jambi, was conducted from November to February 2023. The purpose of this research was to identify pathogenic bacteria in the Batanghari River, Jambi. The research method involved purposive sampling in the field and bacterial identification tests in the laboratory. Samples from the field were diluted, inoculated, and incubated on Nutrient Agar (NA) media for 24 hours. Macroscopic observations included the shape, color, elevation, texture, and surface of the bacterial isolates. Microscopic observations i

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Moore, Cecil P., Nancy Heller, Lynda J. Majors, R. David Whitley, Elizabeth C. Burgess, and JoAnne Weber. "Prevalence of ocular microorganisms in hospitalized and stabled horses." American Journal of Veterinary Research 49, no. 6 (1988): 773–77. https://doi.org/10.2460/ajvr.1988.49.06.773.

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SUMMARY Microorganisms from normal eyes of hospitalized and stabled horses were identified, and the frequency of isolation was compared between the 2 groups. Using standard techniques, swab specimens from both eyes of 22 hospitalized horses and both eyes of 18 stabled horses were cultured for aerobic bacteria and fungi. Ninety-six aerobic bacteria and 57 fungi were isolated. The predominant bacterial isolates were gram-positive organisms, most of which belonged to the genera Corynebacterium, Bacillus, Staphylococcus, and Streptomyces. Gram-negative organisms comprised less than one-fourth of t

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Agustina, Ninik, Eka Nurrahema Ning Asih, and Ary Giri Dwi Kartika. "Jenis Gram dan Morfologi Koloni Bakteri Air Baku Garam." Jurnal Ilmu Kelautan Lesser Sunda 2, no. 1 (2022): 1–8. http://dx.doi.org/10.29303/jikls.v2i1.44.

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Halophilic bacteria are bacteria that can survive in environments with high salt levels, one of which is in salt ponds. Therefore, it is necessary to research to determine the morphology of halophilic bacteria for further use in the identification of bacterial species. This study aimed to determine the colony morphology and types of gram halophilic bacteria. Isolation of halophilic bacteria was carried out using the scatter method. Bacterial purification was carried out by streak method, then morphology identification and bacterial staining were carried out to determine the cell shape and gram

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Shobha, Mehra, Mehra Vimla, and Bhauryal Dinesh. "Isolation, Identification of Probiotic Bacteria Present in Milk." International Journal of Trend in Scientific Research and Development 2, no. 5 (2018): 1173–78. https://doi.org/10.31142/ijtsrd16979.

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The aim ofThe aim of this study was to present some data on isolation, growth, and antimicrobial activity, effect of pH, heat, and sensitivity to proteolytic enzymes of lactobacillus as probiotic bacteria. A large amount of probiotic bacteria is present in milk or milk products which are mainly lactic acid bacteria LAB . Lactic acid bacteria LAB including Lactobacillus spp. Isolation, identification of lactic acid bacterial LAB was done by Gram's staining and catalase test and further confirmation was based on morphological, cultural, physiological and different biochemical tests. A total

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AbdelRahman, Mona, Karim Selim, and Amany Adel. "Comparative Study Between the Sequencing of 16sRNA gene and Traditional Identification Methods of Some Bacterial Pathogens Associated with A Systemic Bacterial Infections from Diseased Poultry Flocks." Alexandria Journal of Veterinary Sciences 74, no. 1 (2022): 24. http://dx.doi.org/10.5455/ajvs.24347.

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Poultry health problems of chickens are usually accompanied by bacterial systemic infections causing severe losses in flocks, sick birds were systemically could be infected by several bacteria regardless of the clinical signs. 16s RNA sequence is a modern approach for the diagnosis of bacterial infection, especially of multiple causative agents. Ten field cases suffering from systemic infections have been examined for bacterial causes, using conventional isolation and 16S rRNA sequencing, The ten sequenced samples were compatible with the results of isolation; six samples were similar to E.col

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Sumbodo, Yoshua Mario, Fiona Aqhila Dewi, Okti Hajeng Kristiadi, et al. "Isolation and Morphological Characterization of Lead Tolerant Bacteria Associated with Perna viridis." Buletin Oseanografi Marina 12, no. 3 (2023): 465–72. http://dx.doi.org/10.14710/buloma.v12i3.59350.

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Lead is a heavy metal contamination that is released into sea waters and cannot be decomposed so it accumulation and magnification along the food chain. Efforts to explore bacteria that have tolerance and have the potential to become lead reduction agents are a strategic step in remediating lead contamination. One mechanism for lead bioremediation by bioaccumulation is to utilize metallothionein protein which can potentially be obtained from isolates of green mussel associated bacteria. Bacterial isolates associated with green mussels were obtained by isolating the bacteria and testing the tol

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Cho, Hye-sun, Munjeong Choi, Yunjung Lee, et al. "High-Quality Nucleic Acid Isolation from Hard-to-Lyse Bacterial Strains Using PMAP-36, a Broad-Spectrum Antimicrobial Peptide." International Journal of Molecular Sciences 22, no. 8 (2021): 4149. http://dx.doi.org/10.3390/ijms22084149.

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The efficiency of existing cell lysis methods to isolate nucleic acids from diverse bacteria varies depending on cell wall structures. This study tested a novel idea of using broad-spectrum antimicrobial peptides to improve the lytic efficiency of hard-to-lyse bacteria and characterized their differences. The lysis conditions of Staphylococcus aureus using recombinant porcine myeloid antimicrobial peptide 36 (PMAP-36), a broad-spectrum pig cathelicidin, was optimized, and RNA isolation was performed with cultured pellets of ten bacterial species using various membranolytic proteins. Additional

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Eichmeier, Aleš, Eliška Peňázová, Jakub Pečenka, et al. "Rapid Communication. Monitoring the occurrence of bacteria in stored cabbage heads." Journal of Plant Protection Research 57, no. 1 (2016): 56–61. http://dx.doi.org/10.1515/jppr-2017-0008.

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Abstract Twenty-six cabbage heads stored under typical conditions in a storage hall in Moravia, Czech Republic, were tested for the presence of bacteria by the method of isolation from three different parts of the cabbage heads. Isolations were carried out from stalks, inner and superficial leaves. Two samplings were done; in November 2015 and February 2016. Bacterial cultures were sequenced in the part of 16S rRNA region; bacteria were identified according to the sequences obtained. The most prevalent bacteria were of the genus Pseudomonas. Genera: Klebsiella, Erwinia, Pantoea, Bacillus were

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Marlyati, Dwi Rinda, Dewi Chusniasih, and Winati Nurhayu. "ANTIOXIDANT ACTIVITY OF ENDOPHYTIC BACTERIA ORIGIN FROM ENDOCARP OF COCOA POD HUSK." Berkala Penelitian Hayati 30, no. 2 (2024): 1–5. http://dx.doi.org/10.23869/bphjbr.30.2.20244.

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Cocoa (Theobroma cacao L.) is one of the leading commodities in Indonesia. Cocoa pod husk is part of the mesocarp or part of the cacao pod husk that wraps the cocoa beans. Endophytic bacteria are microbes that live in host plant tissues and do not interfere with host plant growth. This study aimed to determine characteristics of endophytic bacterial isolates contained in endocarp cocoa pod husk and to determine the antioxidant power of endophytic bacterial isolates contained in cocoa pod husk. Isolation of endophytic bacteria from the inner pod of cocoa which be expected to produce antioxidant

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Qianyi, Tian, Deng Lei, Yin Mi, and Wei Yahong. "ISOLATION AND IDENTIFICATION OF LIGNIN-DEGRADING BACTERIAL STRAINS." Bulletin of Shakarim University. Technical Sciences, no. 3(15) (September 27, 2024): 97–104. http://dx.doi.org/10.53360/2788-7995-2024-3(15)-14.

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Screening bacterial strains with lignin-degrading ability is very necessary for processing agricultural and forestry waste. In this experiment, we collected soil samples from the Qinling Mountains in China and isolated 99 bacterial strains. Further, 18 strains of bacteria were screened out using alkaline lignin solid medium and cultured at 30°C for 48 hours, and they were molecularly identified. After that, lignin liquid culture medium was used for shake flask culture, and a microplate reader was used to measure the absorbance changes of lignin at visible light at 280 nm before and after cultu

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Scalvenzi, Laura, Roldán Torres Gutiérrez, Liliana Cerda Mejía, Andrea Riofrio Carrión, and Manuel Pérez Quintana. "Preliminary Assessment of the In Vitro Immunological Activity of Native Lactobacillus Strains from the Ecuadorian Amazon." Bionatura Journal 1, no. 3 (2024): 1–12. http://dx.doi.org/10.70099/bj/2024.01.03.20.

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The isolation and characterization of new Lactobacillus strains from fermented traditional foods is a global trend as it enhances the potential for discovering novel probiotic foods. The fermented cocoa (Theobroma cacao) mucilage is an intriguing substrate for isolating lactic acid bacteria involved in fermentation. This study represents a preliminary investigation into the isolation, quantification, characterization, and immunological activity of Lactobacillus strains derived from cocoa beans sampled from two farmer associations (Kallari and Wiñak) in the Ecuadorian Amazon region. A mother cu

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Jarullah, Basim. "Isolation and identification of bacterial contamination of raw meat in Thi-Qar province." University of Thi-Qar Journal of Science 5, no. 3 (2015): 13–17. http://dx.doi.org/10.32792/utq/utjsci/v5i3.135.

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Meat considers a favorite environment for bacterial growth due to rich composition with protein, fats, minerals,vitamins and other essential materials requirements for microorganism growth. Study concluded isolation and bacterial account of meat in Thiqar city markets. Study extends from November2014- March 2015. Thirty samples of meat 10 from beef meat, 10 from sheep and 10 from poultry meat). Bacterial account shown that the highest contamination of meat samples found in sheep meat 16*106 CUF/g (more than standard parameters of healthy suitable meat for human consumption) while the lowest ba

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Nuryady, Moh Mirza, Aisha Aisha, Diva Aulia, and Aulia Savitri. "Research trends in isolation and identification of bacteria from Indonesia with various roles: Review Article." Journal of Biotechnology and Natural Science 1, no. 2 (2022): 80–87. http://dx.doi.org/10.12928/jbns.v1i2.5232.

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Bacteria are agents that can be used widely and are genetically easy to manipulate and reproduce. Many studies related to the isolation and identification of bacterial isolates from Indonesia have been carried out for various purposes. This research is still ongoing and has never been informed about the abundance of data from previous studies. The purpose of this study is to provide an overview of research topic trends related to the isolation and identification of bacterial isolates from Indonesia. The method used in this review is by setting inclusion and exclusion criteria and selecting a r

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Al Falah, Inda Hawa. "Isolation and Macroscopic Characterization of Molase and Alginate Bacteria from Mangrove Sediments in Shrimp Ponds." Journal of Marine Biotechnology and Immunology 1, no. 1 (2023): 1–4. http://dx.doi.org/10.61741/v60xdn60.

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Bacteria are microscopic organisms capable of thriving in various environments that support their growth, including mangrove sediment. Bacterial characteristics in mangrove sediment were examined using two different media: molasses and alginate. This process included preparing solid media, diluting sediment samples, isolating bacteria, and characterizing isolates. On molasses media (both Molasses Media I and Molasses Media II), bacteria generally displayed entire, undulate, and lobate margins, with diverse shapes (round, irregular, punctiform, rhizoid), colors (opaque, clear), and elevations (

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Kumar, Virendra, and Archana Shrivastav. "Isolation and Identification of Bacteria from Pharmaceutical Site." International Journal of Current Microbiology and Applied Sciences 12, no. 4 (2023): 184–91. http://dx.doi.org/10.20546/ijcmas.2023.1204.021.

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Antibiotic pollution is an important and extensive environmental problem and even threat to human health. Biodegradation is a major mechanism which removes the pollutants from the environment. Therefore, the present study aimed to screen and isolate antibiotic degrading bacteria which can effectively degrade azithromycin, clarithromycin, cefadroxil and cephalexin antibiotic. Six antibiotic degrading bacteria were isolated from pharmaceutical waste site and the antibiotic degradation rate of each strain was determined by TMSUS method. Bacterial strains were morphologically identified using gram

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Prinz, Christelle, Jonas O. Tegenfeldt, Robert H. Austin, Edward C. Cox, and James C. Sturm. "Bacterial chromosome extraction and isolation." Lab on a Chip 2, no. 4 (2002): 207. http://dx.doi.org/10.1039/b208010a.

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Trevors, J. T. "Bacterial plasmid isolation and purification." Journal of Microbiological Methods 3, no. 5-6 (1985): 259–71. http://dx.doi.org/10.1016/0167-7012(85)90008-9.

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Sunitha, T. Elizabeth Thangamani, Tonio Ranjitha Amritha N., Shunmugap Priya N., and G. Poomari. "Isolation of Bacterial Colonies from House Fly Mouth Parts." UTTAR PRADESH JOURNAL OF ZOOLOGY 45, no. 3 (2024): 241–51. http://dx.doi.org/10.56557/upjoz/2024/v45i33898.

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Housefly (Musca Domestica Li.,1758) is a mechanical vector of pathogens spreading infectious diseases such as Cholera, Shigellosis, Salmonellosis and skin infections in human and domestic animals. The main objective is to isolate the bacteria from housefly mouthparts. The houseflies were collected from different places (sources) such as dairy farms, Fruit stalls, Fish markets and butcher shops located in the surrounding region. The mouth part of the housefly is dissected and it is dipped in saline water. This infested Saline water is diluted at different concentrations using the serial dilutio

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S, Shivalingaiah, Bindushree H.S, Basavaraju G.L, and Pushpalatha H G. "Isolation and Identification of Soil-Derived Amylase-Producing Bacteria." Current Agriculture Research Journal 12, no. 1 (2024): 445–58. http://dx.doi.org/10.12944/carj.12.1.36.

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Soil is a vital ecosystem for various life forms, including bacteria. Bacteria are the main group of soil microbes, mostly because of their ability to produce various extracellular enzymes. Amylases are the enzymes that break down starch, which are essential in biotechnology industries such as food, fermentation, textiles, and paper manufacturing. The demand for amylase is often high since many enterprises do not have access to local supplies of the enzyme. The objective of the current investigation is to isolate and identify bacterial isolates that can produce the enzyme amylase from soil sam

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Pratiwi, Wardha Maulidya, Rana Yumna Nabila, Heliza Amalini, and Guntur Trimulyono. "Isolasi Dan Karakterisasi Bakteri Selulolitik Biopori Sebagai Upaya Awal Percepatan Proses Pengomposan." Biotropic : The Journal of Tropical Biology 4, no. 1 (2020): 48–54. http://dx.doi.org/10.29080/biotropic.2020.4.1.48-54.

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The purpose of this study was to obtain cellulolytic bacterial isolates isolated from leaf litter in absorption holes biopori FMIPA Unesa and obtain the most optimal cellulolytic bacterial isolates in cellulose degradation. This research was an observational study and the data were analyzed descriptively. Stages of the study began with bacterial isolation, cellulolytic ability testing, and characterization of cellulolytic bacterial isolates. Bacterial isolation was carried out by the pour plate method, isolation was carried out by the streak plate method, cellulolytic testing was carried out u

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Pratiwi, Wardha Maulidya, Rana Yumna Nabila, Heliza Amalini, and Guntur Trimulyono. "Isolasi Dan Karakterisasi Bakteri Selulolitik Biopori Sebagai Upaya Awal Percepatan Proses Pengomposan." Biotropic : The Journal of Tropical Biology 4, no. 1 (2020): 48–54. http://dx.doi.org/10.29080/biotropic.2020.4.1.48-54.

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The purpose of this study was to obtain cellulolytic bacterial isolates isolated from leaf litter in absorption holes biopori FMIPA Unesa and obtain the most optimal cellulolytic bacterial isolates in cellulose degradation. This research was an observational study and the data were analyzed descriptively. Stages of the study began with bacterial isolation, cellulolytic ability testing, and characterization of cellulolytic bacterial isolates. Bacterial isolation was carried out by the pour plate method, isolation was carried out by the streak plate method, cellulolytic testing was carried out u

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Najvirt, Biljana, Simonida Djuric, Timea Hajnal-Jafari, and Dragana Stamenov. "Characteristics of agricultural organic matter degrading bacterial isolates from different types of soil." Zbornik Matice srpske za prirodne nauke, no. 136 (2019): 69–78. http://dx.doi.org/10.2298/zmspn1936069n.

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A large amount of agricultural organic matter (AOM) comes into soil every day, through organic remains, and it is decomposed by bacteria, fungi and actinomycetes (Ugarkovic et al, 2011). The aim of this research was to select isolates of bacteria with the most organic matter degrading potential, by isolating the bacteria from five different types of soil. Isolation of bacteria was conducted from five types of soil - luvisol, cambisol, chernozem, forest land and meadow. Characterization of bacterial isolates was conducted based on morphological, physiological and biochemical features. Isolates

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Hong-Xian, Tian, Wang Rui-Xia, Li Yin-Fan, Wang Xiong, Sun Fu-Zai, and Yuan Jun. "Isolation, screening and identification of endophytic antagonistic bacteria toPotato ring rotbacteria." Chinese Journal of Agricultural Biotechnology 2, no. 3 (2005): 173–78. http://dx.doi.org/10.1079/cjb200579.

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AbstractTwo hundred and forty bacterial strains were isolated from three potato (Solanum tuberosum) cultivars from eight different sources. On the basis of antagonistic bacteria examinationin vitro, greenhouse and field tests, 22.9% of isolated bacteria were endophytic and antagonistic. Three strains, namely A-10', T3(Bacillussp.) and H1-6 (Pseudomonas fluorescens), were chosen for their suppressive effect on bacterial ring rot (Clavibacter michiganensissubsp.sepedonicus) and their growth promotion. The strain A-10' was an endophytic bacterium with the effects of colonization, plant growth pro

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Yasin, Rimsha, Sunbul Rasheed, Mohsin Tassawar Cheema, and Aatif Amin. "ISOLATION AND CHARACTERIZATION OF PLANT GROWTH-PROMOTING BACTERIA FROM DIFFERENT RHIZOSPHERIC SOILS." JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS 3, no. 3 (2022): 179–89. http://dx.doi.org/10.52700/jmmg.v3i3.94.

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The production of indole acetic acid (IAA) is a significant property of rhizospheric bacteria to stimulate and promote plant growth. In this investigation, indole acetic acid-producing bacteria from the rhizosphere of five distinct plants (Wheat, Gulchen, Rose, Lady Finger, and Bouginvella) were isolated and characterized. Bacterial strains were isolated and identified by morphological characterization. Out of 30 bacterial isolates, 5 isolates were selected as efficient producers of Indole acetic acid. These selected bacterial isolates were subjected to several biochemical tests for identifica

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Laval, Valérie, Lydie Kerdraon, Matthieu Barret, et al. "Assessing the Cultivability of Bacteria and Fungi from Arable Crop Residues Using Metabarcoding Data as a Reference." Diversity 13, no. 9 (2021): 404. http://dx.doi.org/10.3390/d13090404.

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This study combined culture-dependent (strain isolation plus molecular identification) and culture-independent (metabarcoding) approaches to characterize the diversity of microbiota on wheat and oilseed rape residues. The goal was to develop a methodology to culture microorganisms with the aim of being able to establish synthetic crop residue microbial communities for further study, i.e., testing potential interactions within these communities and characterizing groups of beneficial taxa that could be used as biological control agents against plant pathogens. We generated community-based cultu

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Kopeyev, S. K., Zh K. Kydyrbayev, R. A. Rystayeva, et al. "ISOLATION AND IDENTIFICATION OF CLOSTRIDIUM FROM SAIGAS." Biosafety and Biotechnology, no. 21 (March 31, 2025): 32–43. https://doi.org/10.58318/2957-5702-2025-21-32-43.

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Based on the results of an epizootological survey of the area and microbiological studies of pathological material from saigas brought from the West Kazakhstan region during the epizootics of 2010 and 2011, the cause of the mass death of saigas was established. Gram-positive bacteria with spores, located predominantly subterminally, were found in fingerprint smears from the internal organs of a fallen saiga. Bacteriological nutrient media show the growth of bacterial colonies in the form of intense turbidity (MPA), uniform turbidity (Kitt-Tarozzi) with abundant gas formation, colonies of small

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Rajamanickam, Venkatalaxmi, Aruna Sunder, and C. Prathiba. "ISOLATION, IDENTIFICATION OF BACTERIAL AGENT CAUSING KERATITIS, ANTIBIOTIC SENSITIVITY TESTING." International Journal of Integrative Medical Sciences 5, no. 4 (2018): 607–12. http://dx.doi.org/10.16965/ijims.2018.107.

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