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1

Takenaka, Yukinori, Kazuya Takeda, Tadashi Yoshii, Michiko Hashimoto, and Hidenori Inohara. "Gram Staining for the Treatment of Peritonsillar Abscess." International Journal of Otolaryngology 2012 (2012): 1–5. http://dx.doi.org/10.1155/2012/464973.

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Objective. To examine whether Gram staining can influence the choice of antibiotic for the treatment of peritonsillar abscess.Methods. Between 2005 and 2009, a total of 57 cases of peritonsillar abscess were analyzed with regard to cultured bacteria and Gram staining.Results. Only aerobes were cultured in 16% of cases, and only anaerobes were cultured in 51% of cases. Mixed growth of aerobes and anaerobes was observed in 21% of cases. The cultured bacteria were mainly aerobicStreptococcus, anaerobic Gram-positive cocci, and anaerobic Gram-negative rods. Phagocytosis of bacteria on Gram stainin
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2

Greiner, Martina, Georg Wolf, and Katrin Hartmann. "Bacteraemia in 66 cats and antimicrobial susceptibility of the isolates (1995–2004)." Journal of Feline Medicine and Surgery 9, no. 5 (2007): 404–10. http://dx.doi.org/10.1016/j.jfms.2007.04.004.

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Bacterial blood culture results of 292 privately owned cats presented to the Clinic for Small Animal Medicine, Ludwig Maximilian University Munich with signs of sepsis were evaluated retrospectively. Of the blood cultures, 23% were positive. In 88%, a single bacterial species was isolated. Of all bacterial isolates, 45% were Gram-positive, 43% were Gram-negative, and 12% were obligate anaerobes. The most frequently isolated bacteria were Enterobacteriaceae, obligate anaerobic species, Staphylococcus species and Streptococcus species. Of the cats with positive blood cultures, 32% were pretreate
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3

Ghebremedhin, Beniam. "Bacterial Infections in the Elderly Patient: Focus on Sitafloxacin." Clinical Medicine Insights: Therapeutics 4 (January 2012): CMT.S7435. http://dx.doi.org/10.4137/cmt.s7435.

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Sitafloxacin (DU-6859a) is a new-generation oral fluoroquinolone with in vitro activity against a broad range of Gram-positive and -negative bacteria, including anaerobic bacteria, as well as against atypical bacterial pathogens. Particularly in Japan this antibiotic was approved in 2008 for treatment of a number of bacterial infections caused by Gram-positive cocci and Gram-negative cocci and rods, including anaerobia atypical bacterial pathogens. As compared to oral levofloxacin sitafloxacin was non-inferior in the treatment of community-acquired pneumonia and non-inferior in the treatment o
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4

Jousimies-Somer, H., S. Pyörälä, and A. Kanervo. "Susceptibilities of bovine summer mastitis bacteria to antimicrobial agents." Antimicrobial Agents and Chemotherapy 40, no. 1 (1996): 157–60. http://dx.doi.org/10.1128/aac.40.1.157.

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The susceptibility to 9 antimicrobial agents of 32 aerobic bacterial isolates and to 10 antimicrobial agents of 37 anaerobic bacterial isolates from 23 cases of bovine summer mastitis (16 Actinomyces pyogenes isolates, 8 Streptococcus dysgalactiae isolates, 3 S. uberis isolates, 3 S. acidominimus isolates, 2 Streptococcus spp., 15 Peptostreptococcus indolicus isolates, 10 Fusobacterium necrophorum isolates, and 12 isolates of anaerobic gram-negative rods) was determined by the agar dilution method. All isolates except one Bacteroides fragilis isolate (beta-lactamase producer) were susceptible
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5

Myers, Catherine, Yacine Aggoun, Alain Gervaix, Beatrice Ninet, Jacques Schrenzel, and Peter Kuhnert. "Postoperative Gram-Negative Anaerobic Bacterial Endocarditis." Pediatric Infectious Disease Journal 26, no. 4 (2007): 369. http://dx.doi.org/10.1097/01.inf.0000258694.43509.05.

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6

Finegold, Sydney M., Mauricio Bolanos, Paula H. Sumannen, and Denise R. Molitoris. "In Vitro Activities of Telavancin and Six Comparator Agents against Anaerobic Bacterial Isolates." Antimicrobial Agents and Chemotherapy 53, no. 9 (2009): 3996–4001. http://dx.doi.org/10.1128/aac.00908-08.

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ABSTRACT The antimicrobial activities of telavancin and six comparators were evaluated against 460 isolates of anaerobic bacteria. Telavancin demonstrated excellent activity against gram-positive anaerobes (MIC90, 2 μg/ml) and was the most potent agent tested against Clostridium difficile (MIC90, 0.25 μg/ml). As expected, gram-negative isolates were not inhibited by telavancin.
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7

Harvey, C. E., C. Thornsberry, and B. R. Miller. "Subgingival Bacteria - Comparison of Culture Results in Dogs and Cats with Gingivitis." Journal of Veterinary Dentistry 12, no. 4 (1995): 147–50. http://dx.doi.org/10.1177/089875649501200405.

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Aerobic and anaerobic subgingival bacteria were cultured and identified from 49 dogs and 40 cats with spontaneous gingivitis. The most common organisms were gram-negative anaerobes (37% of canine isolates and 39% of feline isolates) and gram-positive aerobes (36% of canine isolates and 29% of feline isolates). No major differences were found between the subgingival floras of dogs and cats with gingivitis.
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8

Cobo, Fernando, Vicente Guillot, and José María Navarro-Marí. "Breast Abscesses Caused by Anaerobic Microorganisms: Clinical and Microbiological Characteristics." Antibiotics 9, no. 6 (2020): 341. http://dx.doi.org/10.3390/antibiotics9060341.

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The objectives of this study were to report the antimicrobial susceptibility of 35 clinically significant anaerobic bacteria isolated from breast abscesses between March 2017 and February 2020 in a tertiary hospital in Granada (Spain) and to describe key clinical features of the patients. Species identification was performed mainly by MALDI-TOF MS. Antimicrobial susceptibility tests were carried out against benzylpenicillin, amoxicillin–clavulanic acid, imipenem, moxifloxacin, clindamycin, metronidazole, and piperacillin–tazobactam using the gradient diffusion technique and European Committee
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9

Harvey, C. E., C. Thornsberry, B. R. Miller, and F. S. Shofer. "Antimicrobial Susceptibility of Subgingival Bacterial Flora in Dogs with Gingivitis." Journal of Veterinary Dentistry 12, no. 4 (1995): 150–55. http://dx.doi.org/10.1177/089875649501200407.

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The aerobic and anaerobic flora from gingival pockets of 49 dogs with severe gingivitis and periodontitis were cultured. The susceptibility of each isolate to four antimicrobial agents currently approved for veterinary use in the USA (amoxicillin-clavulanic acid; clindamycin; cefadroxil; and enrofloxacin) was determined. Amoxicillin-clavulanic acid (Clavamox® Pfizer Animal Health) had the highest in-vitro susceptibility against all isolates (96%), all aerobes (94%) and all anaerobes (100%) tested. For gram-negative aerobes, enrofloxacin (Baytril®, Bayer Corp.) had the highest in-vitro suscepti
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10

Kleinkauf, Niels, Grit Ackermann, Reiner Schaumann, and Arne C. Rodloff. "Comparative In Vitro Activities of Gemifloxacin, Other Quinolones, and Nonquinolone Antimicrobials against Obligately Anaerobic Bacteria." Antimicrobial Agents and Chemotherapy 45, no. 6 (2001): 1896–99. http://dx.doi.org/10.1128/aac.45.6.1896-1899.2001.

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ABSTRACT The in vitro activity of gemifloxacin was compared to that of other quinolone and nonquinolone antimicrobials against 204 anaerobes by the agar dilution technique. The data indicate that gemifloxacin has a rather selective anaerobic activity. Most Peptostreptococcus, Porphyromonas, and Fusobacterium species are susceptible, while gemifloxacin's activity against other gram-negative anaerobes appears to be variable.
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11

Brook, Itzhak, and Kiran Shah. "Sinusitis in neurologically impaired children." Otolaryngology–Head and Neck Surgery 119, no. 4 (1998): 357–60. http://dx.doi.org/10.1016/s0194-5998(98)70078-6.

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The microbiologic features of infected sinus aspirates in nine children with neurologic impairment were studied. Anaerobic bacteria, always mixed with aerobic and facultative bacteria, were isolated in 6 (67%) aspirates and aerobic bacteria only in 3 (33%). There were 24 bacterial isolates, 12 aerobic or facultative and 12 anaerobic. The predominant aerobic isolates were Klebsiella pneumoniae, Escherichia coli, and Staphylococcus aureus (2 each) and Proteus mirabilis, Pseudomonas aeruginosa, Haemophilus influenzae, Moraxella catarrhalis, and Streptococcus pneumoniae (1 each). The predominant a
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12

Hessle, Christina, Bengt Andersson, and Agnes E. Wold. "Gram-Positive Bacteria Are Potent Inducers of Monocytic Interleukin-12 (IL-12) while Gram-Negative Bacteria Preferentially Stimulate IL-10 Production." Infection and Immunity 68, no. 6 (2000): 3581–86. http://dx.doi.org/10.1128/iai.68.6.3581-3586.2000.

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ABSTRACT Interleukin-10 (IL-10) and IL-12 are two cytokines secreted by monocytes/macrophages in response to bacterial products which have largely opposite effects on the immune system. IL-12 activates cytotoxicity and gamma interferon (IFN-γ) secretion by T cells and NK cells, whereas IL-10 inhibits these functions. In the present study, the capacities of gram-positive and gram-negative bacteria to induce IL-10 and IL-12 were compared. Monocytes from blood donors were stimulated with UV-killed bacteria from each of seven gram-positive and seven gram-negative bacterial species representing bot
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13

Almansa Ruiz, José Carlos, Annelize Jonker, Anna-Mari Bosman, and Gerhard Steenkamp. "Bacteria profile and antibiogram of the bacteria isolated from the exposed pulp of dog canine teeth." Veterinary Record 183, no. 3 (2018): 97. http://dx.doi.org/10.1136/vr.104540.

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Twenty-seven microbiological samples were taken from root canals (RC) of the canine teeth of 20 dogs where the pulps were non-vital and exposed due to complicated crown fractures. These pulps were cultured for aerobic/anaerobic bacteria. Antimicrobial susceptibility of isolates was determined using the Kirby-Bauer diffusion test. A total of 49 cultivable isolates, belonging to 27 different microbial species and 18 different genera, were recovered from the 27 RCs sampled. Twenty (40.81 per cent) of the cultivable isolates were Gram positive while 29 (59.19 per cent) were Gram negative. Facultat
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14

Chelack, Brian J., and W. M. Ingledew. "Anaerobic Gram-Negative Bacteria in Brewing—A Review." Journal of the American Society of Brewing Chemists 45, no. 4 (1987): 123–27. http://dx.doi.org/10.1094/asbcj-45-0123.

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15

Wexler, Hannah M. "Pore‐Forming Molecules in Gram‐Negative Anaerobic Bacteria." Clinical Infectious Diseases 25, s2 (1997): S284—S286. http://dx.doi.org/10.1086/516225.

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16

Citron, D. M., K. L. Tyrrell, C. V. Merriam, and E. J. C. Goldstein. "In Vitro Activity of Ceftaroline against 623 Diverse Strains of Anaerobic Bacteria." Antimicrobial Agents and Chemotherapy 54, no. 4 (2010): 1627–32. http://dx.doi.org/10.1128/aac.01788-09.

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ABSTRACT The in vitro activities of ceftaroline, a novel, parenteral, broad-spectrum cephalosporin, and four comparator antimicrobials were determined against anaerobic bacteria. Against Gram-positive strains, the activity of ceftaroline was similar to that of amoxicillin-clavulanate and four to eight times greater than that of ceftriaxone. Against Gram-negative organisms, ceftaroline showed good activity against β-lactamase-negative strains but not against the members of the Bacteroides fragilis group. Ceftaroline showed potent activity against a broad spectrum of anaerobes encountered in res
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17

Brook, Itzhak. "Recovery of Aerobic and Anaerobic Bacteria in Sinus Fungal Ball." Otolaryngology–Head and Neck Surgery 145, no. 5 (2011): 851–52. http://dx.doi.org/10.1177/0194599811417066.

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This case study with chart review describes the aerobic and anaerobic microbiology of specimens obtained from 47 patients with Aspergillus spp fungus ball. Bacteria were recovered from 32 of the 47 (68%) patients. Eighty-six isolates, 29 aerobic and facultatives and 57 anaerobic, were recovered. Aerobic and facultatives only were recovered in 6 instances (19% of culture-positive specimens), anaerobes only in 11 (34%), and mixed aerobes and anaerobes in 15 (47%). The predominant aerobes were Staphylococcus aureus (6 isolates), α-hemolytic streptococci (5 isolates), Enterobacteriacae (4 isolates
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18

Wexler, Hannah M., Denise Molitoris, Shahera St. John, Ann Vu, Erik K. Read, and Sydney M. Finegold. "In Vitro Activities of Faropenem against 579 Strains of Anaerobic Bacteria." Antimicrobial Agents and Chemotherapy 46, no. 11 (2002): 3669–75. http://dx.doi.org/10.1128/aac.46.11.3669-3675.2002.

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ABSTRACT The activity of faropenem, a new oral penem, was tested against 579 strains of anaerobic bacteria by using the NCCLS-approved reference method. Drugs tested included amoxicillin-clavulanate, cefoxitin, clindamycin, faropenem, imipenem, and metronidazole. Of the 176 strains of Bacteroides fragilis group isolates tested, two isolates had faropenem MICs of 64 μg/ml and imipenem MICs of >32 μg/ml. Faropenem had an MIC of 16 μg/ml for an additional isolate of B. fragilis; this strain was sensitive to imipenem (MIC of 1 μg/ml). Both faropenem and imipenem had MICs of ≤4 μg/ml for all iso
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19

Peycheva, Stela K., Elisaveta G. Apostolova, Zhivko L. Peychev, Petya A. Gardjeva, Mihaela S. Shishmanova-Doseva, and Marianna A. Murdjeva. "Oral Microbial Flora in Bulgarian Adolescents with Moderate Plaque-induced Gingivitis." Folia Medica 61, no. 4 (2019): 522–28. http://dx.doi.org/10.3897/folmed.61.e47734.

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Introduction: In children and adolescents, the most common periodontal disease is the plaque-induced gingivitis.Aim: The aim of this study was to reveal the bacterial species associated with supragingival plaque of Bulgarian adolescents diagnosed with plaque-induced gingivitis.Materials and methods: Supragingival plaque samples from 70 healthy subjects with moderate plaque-induced gingivitis (37 females and 33 males), aged 12-18 years, were obtained and examined microbiologically.Results: A total of 224 microorganisms were isolated. Gram-negative bacteria were predominant compared to Gram-posi
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20

Zurak, Kreso, Davor Vagić, Petar Drvis, Carmen Prohaska Potocnik, Senka Dzidic, and Livije Kalogjera. "Bacterial colonization and granulocyte activation in chronic maxillary sinusitis in asthmatics and non-asthmatics." Journal of Medical Microbiology 58, no. 9 (2009): 1231–35. http://dx.doi.org/10.1099/jmm.0.010579-0.

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The impact of bacterial colonization on the severity and pattern of chronic inflammation in rhinosinusitis is not clear. In this study, it was hypothesized that bacterial colonization of the sinus mucosa would have a greater impact on inflammatory response modulation in asthmatic patients than in non-asthmatic patients with chronic rhinosinusitis. In order to test this hypothesis, granulocyte activation was measured and related to bacteria identified in the sinus lavage. Lavages from the maxillary sinuses of 21 asthmatic and 19 non-asthmatic patients with chronic rhinosinusitis (CRS) were micr
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21

Brook, Itzhak. "Bacteriology of Acute and Chronic Sphenoid Sinusitis." Annals of Otology, Rhinology & Laryngology 111, no. 11 (2002): 1002–4. http://dx.doi.org/10.1177/000348940211101109.

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Aspirates of 16 acutely infected and 7 chronically infected sphenoid sinuses were processed for aerobic and anaerobic bacteria. A total of 29 isolates were recovered from the 16 cases of acute sphenoid sinusitis (1.8 per specimen): 22 aerobic and facultative (1.4 per specimen), and 7 anaerobic (0.4 per specimen). Aerobic and facultative organisms alone were recovered in 10 specimens (62%), anaerobes alone were isolated in 3 (19%), and mixed aerobic and anaerobic bacteria were recovered in 3 (19%). The predominant aerobic and facultative species were Staphylococcus aureus (9 isolates), Streptoc
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22

Badr, Mohamed Tarek, Benjamin Blümel, Sandra Baumgartner, Johanna M. A. Komp, and Georg Häcker. "Antimicrobial Susceptibility Patterns and Wild-Type MIC Distributions of Anaerobic Bacteria at a German University Hospital: A Five-Year Retrospective Study (2015–2019)." Antibiotics 9, no. 11 (2020): 823. http://dx.doi.org/10.3390/antibiotics9110823.

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Local antimicrobial susceptibility surveys are crucial for optimal empirical therapy guidelines and for aiding in antibiotic stewardship and treatment decisions. For many laboratories, a comprehensive overview of local antimicrobial susceptibility patterns of anaerobic bacteria is still lacking due to the long incubation time and effort involved. The present study investigates the antimicrobial susceptibility patterns and related clinical and demographic data of 2856 clinical isolates of anaerobic bacteria that were submitted for analysis to the Institute for Medical Microbiology and Hygiene o
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Wilcox, C. M., K. B. Waites, and P. D. Smith. "No relationship between gastric pH, small bowel bacterial colonisation, and diarrhoea in HIV-1 infected patients." Gut 44, no. 1 (1999): 101–5. http://dx.doi.org/10.1136/gut.44.1.101.

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Background/AimsConclusive studies of small bowel bacterial overgrowth in patients with HIV-1 infection are limited. The relation was therefore determined between the quantity and species of bacteria in the proximal small intestine of HIV-1 infected patients and the presence of diarrhoea, gastric acidity, severity of immune deficiency, and clinical outcome.MethodsBacteria in the duodenal fluids obtained endoscopically from 32 HIV-1 infected patients, 21 of whom had diarrhoea, and seven control subjects without HIV-1 risk factors were quantified and speciated. Gastric pH was determined at the ti
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24

Patyka, V., L. Butsenko, and L. Pasichnyk. "Application of commercial test-systems to identify gram-negative facultatively anaerobic bacteria." Agricultural Science and Practice 3, no. 1 (2016): 43–48. http://dx.doi.org/10.15407/agrisp3.01.043.

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Aim. To validate the suitability of commercial API 20E test-system (bioMerieux) for the identifi cation and characterization of facultative gram-negative phytopathogenic bacterial isolates. Methods. Conventional mi- crobiological methods, API 20E test-system (bioMerieux) according to the manufacturer’s instructions. Re- sults. The identifi cation results for Erwinia amylovora, Pectobacterium carotovorum and Pantoea agglome- rans isolates were derived from the conventional and API 20E test systems, which, were in line with the literature data for these species. The API 20E test-system showed hi
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25

Mysak, Jaroslav, Stepan Podzimek, Pavla Sommerova, et al. "Porphyromonas gingivalis: Major Periodontopathic Pathogen Overview." Journal of Immunology Research 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/476068.

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Porphyromonas gingivalisis a Gram-negative oral anaerobe that is involved in the pathogenesis of periodontitis and is a member of more than 500 bacterial species that live in the oral cavity. This anaerobic bacterium is a natural member of the oral microbiome, yet it can become highly destructive (termed pathobiont) and proliferate to high cell numbers in periodontal lesions: this is attributed to its arsenal of specialized virulence factors. The purpose of this review is to provide an overview of one of the main periodontal pathogens—Porphyromonas gingivalis.This bacterium, along withTreponem
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26

Bamarouf, A., A. Eley, and T. Winstanley. "Evaluation of Methods for Distinguishing Gram-Positive from Gram-Negative Anaerobic Bacteria." Anaerobe 2, no. 3 (1996): 163–68. http://dx.doi.org/10.1006/anae.1996.0021.

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27

Boetius Hertz, Frederik, Anders Løbner-Olesen, and Niels Frimodt-Møller. "Antibiotic Selection of Escherichia coli Sequence Type 131 in a Mouse Intestinal Colonization Model." Antimicrobial Agents and Chemotherapy 58, no. 10 (2014): 6139–44. http://dx.doi.org/10.1128/aac.03021-14.

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ABSTRACTThe ability of different antibiotics to select for extended-spectrum β-lactamase (ESBL)-producingEscherichia coliremains a topic of discussion. In a mouse intestinal colonization model, we evaluated the selective abilities of nine common antimicrobials (cefotaxime, cefuroxime, dicloxacillin, clindamycin, penicillin, ampicillin, meropenem, ciprofloxacin, and amdinocillin) against a CTX-M-15-producingE. colisequence type 131 (ST131) isolate with a fluoroquinolone resistance phenotype. Mice (8 per group) were orogastrically administered 0.25 ml saline with 108CFU/mlE. coliST131. On that s
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28

Obst, Martin, Andreas Krug, Heinrich Luftmann, and Alexander Steinbüchel. "Degradation of Cyanophycin by Sedimentibacter hongkongensis Strain KI and Citrobacter amalonaticus Strain G Isolated from an Anaerobic Bacterial Consortium." Applied and Environmental Microbiology 71, no. 7 (2005): 3642–52. http://dx.doi.org/10.1128/aem.71.7.3642-3652.2005.

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ABSTRACT Using a combination of various enrichment techniques, the strictly anaerobic, gram-positive, endospore-forming bacterium Sedimentibacter hongkongensis strain KI as revealed by 16S rRNA analysis and the gram-negative enterobacterium Citrobacter amalonaticus strain G as revealed by physiological tests were isolated from an anaerobic cyanophycin (CGP)-degrading bacterial consortium. S. hongkongensis strain KI is the first anaerobic bacterium with the ability to hydrolyze CGP to β-Asp-Arg and β-Asp-Lys dipeptides, as revealed by electrospray ionization-mass spectrometry and reversed-phase
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29

Mombelli, A. "Microbiology of the Dental Implant." Advances in Dental Research 7, no. 2 (1993): 202–6. http://dx.doi.org/10.1177/08959374930070021201.

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Longitudinal studies have shown that successful implants are colonized by a predominantly Gram-positive, facultative flora, which is established shortly after implantation. Repeated microbiological sampling in patients with clinically stable implants showed no significant shifts in the composition of this flora over five years. In patients with bone loss and pocket formation around implants, however, a significantly different flora was found: Gram-negative anaerobic bacteria, particularly fusobacteria, spirochetes, and black-pigmenting organisms such as Prevotella intermedia were often present
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Brook, Itzhak, and G. David Ledney. "Use of Selective Decontamination in the Prevention of Infection After Accidental Irradiation." Prehospital and Disaster Medicine 8, no. 1 (1993): 85–88. http://dx.doi.org/10.1017/s1049023x00040073.

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AbstractExposure to radiation induces a reduction in the number of gastrointestinal, anaerobic bacterial flora, and an increase in the number of Enterobacteriaceae that are associated with sepsis and mortality. Antimicrobials that suppress anaerobic flora have a deleterious effect on survival by promoting earlier enterobacterial sepsis. In contrast, in studies of animals and immunosuppressed patients, antimicrobials that inhibit gram-negative enteric bacteria and preserve the anaerobic flora have shown a beneficial effect by preventing bacterial translocation and fatal sepsis. The quinolone an
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31

Ziola, Barry, Sheryl L. Gares, Brandene Lorrain, Lori Gee, W. M. Ingledew, and Sun Y. Lee. "Epitope mapping of monoclonal antibodies specific for the directly cross-linkedmeso-diaminopimelic acid peptidoglycan found in the anaerobic beer spoilage bacteriumPectinatus cerevisiiphilus." Canadian Journal of Microbiology 45, no. 9 (1999): 779–85. http://dx.doi.org/10.1139/w99-071.

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Nineteen monoclonal antibodies (Mabs) were isolated based on reactivity with disrupted Pectinatus cerevisiiphilus cells. All of the Mabs reacted with cells from which the outer membrane had been stripped by incubation with sodium dodecyl sulphate, suggesting the peptidoglycan (PG) layer was involved in binding. Mab reactivity with purified PG confirmed this. Epitope mapping revealed the Mabs in total recognize four binding sites on the PG. Mabs specific for each of the four sites also bound strongly to disrupted Pectinatus frisingensis, Selenomonas lacticifix, Zymophilus paucivorans, and Zymop
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Balasooriya, W. K., K. Denef, J. Peters, N. E. C. Verhoest, and P. Boeckx. "Vegetation composition and soil microbial community structural changes along a wetland hydrological gradient." Hydrology and Earth System Sciences 12, no. 1 (2008): 277–91. http://dx.doi.org/10.5194/hess-12-277-2008.

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Abstract. Fluctuations in wetland hydrology create an interplay between aerobic and anaerobic conditions, controlling vegetation composition and microbial community structure and activity in wetland soils. In this study, we investigated the vegetation composition and microbial community structural and functional changes along a wetland hydrological gradient. Two different vegetation communities were distinguished along the hydrological gradient; Caricetum gracilis at the wet depression and Arrhenatheretum elatioris at the drier upper site. Microbial community structural changes were studied by
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Finegold, Sydney M., Denise Molitoris, Marja-Liisa Vaisanen, Yuli Song, Chengxu Liu, and Mauricio Bolaños. "In Vitro Activities of OPT-80 and Comparator Drugs against Intestinal Bacteria." Antimicrobial Agents and Chemotherapy 48, no. 12 (2004): 4898–902. http://dx.doi.org/10.1128/aac.48.12.4898-4902.2004.

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ABSTRACT The activities of OPT-80 against 453 intestinal bacteria were compared with those of seven other drugs. OPT-80 showed good activity against most clostridia, staphylococci, and enterococci, but streptococci, aerobic and facultative gram-negative rods, anaerobic gram-negative rods, and Clostridium ramosum were resistant. Poor activity against anaerobic gram-negative rods may maintain colonization resistance.
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Srečnik, Špela, Irena Zdovc, Urška Javoršek, Tina Pirš, Zlatko Pavlica, and Ana Nemec. "Microbiological Aspects of Naturally Occurring Primary Endodontic Infections in Dogs." Journal of Veterinary Dentistry 36, no. 2 (2019): 124–28. http://dx.doi.org/10.1177/0898756419873639.

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Dental fractures are common in dogs, but data on microbiology of naturally occurring primary endodontic infections, and their relation to clinical and radiographic signs, are lacking. Samples were obtained from root canals of 32 periodontally healthy fractured teeth under aseptic conditions and immediately cultured for aerobic and anaerobic bacteria. Cultures were further identified by matrix-assisted laser desorption/ionization, time of flight technology. Sixty-one bacteria (30 bacterial species) were isolated from root canals; 54% were Gram-negative bacteria, 53% were facultative anaerobic,
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Gough, Rory, Joel Barratt, Damien Stark, and John Ellis. "Diversity profiling of xenic cultures of Dientamoeba fragilis following systematic antibiotic treatment and prospects for genome sequencing." Parasitology 147, no. 1 (2019): 29–38. http://dx.doi.org/10.1017/s0031182019001173.

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AbstractThe presence of bacterial DNA in Dientamoeba fragilis DNA extracts from culture poses a substantial challenge to sequencing the D. fragilis genome. However, elimination of bacteria from D. fragilis cultures has proven difficult in the past, presumably due to its dependence on some unknown prokaryote/s. This study explored options for removal of bacteria from D. fragilis cultures and for the generation of genome sequence data from D. fragilis. DNA was extracted from human faecal samples and xenic D. fragilis cultures. Extracts were subjected to 16S ribosomal DNA bacterial diversity prof
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Patil, Swati V., and Roshan R. Mane. "Bacterial and clinical profile of diabetic foot ulcer using optimal culture techniques." International Journal of Research in Medical Sciences 5, no. 2 (2017): 496. http://dx.doi.org/10.18203/2320-6012.ijrms20170139.

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Background: Diabetic foot ulcers (DFU) are the complications of diabetes mellitus. The diabetic foot ulcer infections are polymicrobial in nature. If they are not recognized and controlled it leads to many devastating consequences like limb amputation, sepsis, and even mortality. Hence, the present study was undertaken to determine the bacterial and clinical profile of diabetic foot ulcer using optimal culture techniques and the antimicrobial sensitivity pattern of the isolates.Methods: A total number of 103 patients with a foot ulcer of Wagner’s grade II or more and evidence of purulent exuda
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Naito, Mariko, Keiko Sato, Mikio Shoji, et al. "Characterization of the Porphyromonas gingivalis conjugative transposon CTnPg1: determination of the integration site and the genes essential for conjugal transfer." Microbiology 157, no. 7 (2011): 2022–32. http://dx.doi.org/10.1099/mic.0.047803-0.

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In our previous study, extensive genomic rearrangements were found in two strains of the Gram-negative anaerobic bacterium Porphyromonas (Por.) gingivalis, and most of these rearrangements were associated with mobile genetic elements such as insertion sequences and conjugative transposons (CTns). CTnPg1, identified in Por. gingivalis strain ATCC 33277, was the first complete CTn reported for the genus Porphyromonas. In the present study, we found that CTnPg1 can be transferred from strain ATCC 33277 to another Por. gingivalis strain, W83, at a frequency of 10−7 to 10−6. The excision of CTnPg1
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Silva, Natália S., Ana Carolina Borsanelli, Elerson Gaetti-Jardim Júnior, et al. "Subgingival bacterial microbiota associated with ovine periodontitis." Pesquisa Veterinária Brasileira 39, no. 7 (2019): 454–59. http://dx.doi.org/10.1590/1678-5150-pvb-5913.

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ABSTRACT: Periodontitis is an inflammatory response in a susceptible host caused by complex microbiota, predominantly composed of Gram-negative anaerobic bacteria. Aiming to characterize the subgingival bacterial microbiota associated with ovine periodontitis, polymerase chain reaction (PCR) was performed in subgingival periodontal pocket samples of 14 sheep with severe periodontitis and in subgingival sulcus biofilm of 14 periodontally healthy sheep in search mainly of Gram-negative and Gram-positive microorganisms considered important periodontopathogens. The most prevalent bacteria in the s
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Boyanova, Lyudmila, Rossen Kolarov, Galina Gergova, et al. "Anaerobic bacteria in 118 patients with deep-space head and neck infections from the University Hospital of Maxillofacial Surgery, Sofia, Bulgaria." Journal of Medical Microbiology 55, no. 9 (2006): 1285–89. http://dx.doi.org/10.1099/jmm.0.46512-0.

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The aim of this study was to assess the incidence and susceptibility to antibacterial agents of anaerobic strains in 118 patients with head and neck abscesses (31) and cellulitis (87). Odontogenic infection was the most common identified source, occurring in 73 (77.7 %) of 94 patients. The incidence of anaerobes in abscesses and cellulitis was 71 and 75.9 %, respectively, and that in patients before (31 patients) and after (87) the start of empirical treatment was 80.6 and 72.4 %, respectively. The detection rates of anaerobes in patients with odontogenic and other sources of infection were 82
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Machnicka, Alicja. "Escherichia Coli in Sewage Sludge - Detection Method / Escherichia Coli W Osadach Ściekowych - Metoda Wykrywania." Chemistry-Didactics-Ecology-Metrology 19, no. 1-2 (2014): 79–85. http://dx.doi.org/10.1515/cdem-2014-0007.

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Abstract Escherichia coli is Gram-negative optionally anaerobic roads which belongs to Enterobacteriaceae family. Includes in a physiological bacterial flora of human and warm-blooded animals large intestine. Escherichia coli is being met in abiotic elements of the environment so as waters, wastewater, sewage sludge, soil and the food. This bacterium is showing the pathogenicity in named terms for the peoples, triggering diseases mainly: gastrointestinal tract and urinary. Quality and quantitative proposed detections method of the bacteria E. coli contains five/six steps: - appointment dry sus
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OLMSTED, STUART S., LESLIE A. MEYN, LISA C. ROHAN, and SHARON L. HILLIER. "Glycosidase and Proteinase Activity of Anaerobic Gram-Negative Bacteria Isolated From Women With Bacterial Vaginosis." Sexually Transmitted Diseases 30, no. 3 (2003): 257–61. http://dx.doi.org/10.1097/00007435-200303000-00016.

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42

Balasooriya, W. K., K. Denef, J. Peters, N. E. C. Verhoest, and P. Boeckx. "Vegetation composition and soil microbial community structural changes along a wetland hydrological gradient." Hydrology and Earth System Sciences Discussions 4, no. 5 (2007): 3869–907. http://dx.doi.org/10.5194/hessd-4-3869-2007.

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Abstract. Fluctuations in wetland hydrology create an interplay between aerobic and anaerobic conditions, controlling vegetation composition and microbial community structure and activity in wetland soils. In this study, we investigated the vegetation composition and microbial community structural and functional changes along a wetland hydrological gradient. Two different vegetation communities were distinguished along the hydrological gradient; \\textit{Caricetum gracilis} at the wet depression and \\textit{Arrhenatherum elatioris} at the drier upper site. Microbial community structural chang
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43

Citron, Diane M., Kerin L. Tyrrell, C. Vreni Merriam, and Ellie J. C. Goldstein. "ComparativeIn VitroActivities of LFF571 against Clostridium difficile and 630 Other Intestinal Strains of Aerobic and Anaerobic Bacteria." Antimicrobial Agents and Chemotherapy 56, no. 5 (2012): 2493–503. http://dx.doi.org/10.1128/aac.06305-11.

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ABSTRACTThein vitroactivities of LFF571, a novel analog of GE2270A that inhibits bacterial growth by binding with high affinity for protein synthesis elongation factor Tu, fidaxomicin, and 10 other antimicrobial agents were determined against 50 strains ofClostridium difficileand 630 other anaerobic and aerobic organisms of intestinal origin. LFF571 possesses potent activity againstC. difficileand most other Gram-positive anaerobes (MIC90, ≤0.25 μg/ml), with the exception of bifidobacteria and lactobacilli. The MIC90s for aerobes, including enterococci,Staphylococcus aureus(as well as methicil
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Flamm, R. K., C. Vojtko, D. T. Chu, et al. "In vitro evaluation of ABT-719, a novel DNA gyrase inhibitor." Antimicrobial Agents and Chemotherapy 39, no. 4 (1995): 964–70. http://dx.doi.org/10.1128/aac.39.4.964.

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ABT-719 (A-86719.1) is the first compound of a new class of novel DNA gyrase inhibitors, the 2-pyridones, with potent antibacterial activity against gram-positive, gram-negative, and anaerobic organisms. ABT-719 was more active than ciprofloxacin, sparfloxacin, and clinafloxacin against gram-positive bacteria. ABT-719 was particularly active against Staphylococcus aureus (MIC at which 90% of the isolates were inhibited [MIC90] = 0.015 micrograms/ml) and Streptococcus pneumoniae (MIC90 = 0.03 micrograms/ml). ABT-719 was also the most active of the compounds tested against ciprofloxacin-resistan
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Poxton, Ian R., Robert Brown, Af Sawyerr, and Anne Ferguson. "The Mucosal Anaerobic Gram‐Negative Bacteria of the Human Colon." Clinical Infectious Diseases 25, s2 (1997): S111—S113. http://dx.doi.org/10.1086/516189.

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46

Moncla, B. J., P. Braham, and S. L. Hillier. "Sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria." Journal of Clinical Microbiology 28, no. 3 (1990): 422–25. http://dx.doi.org/10.1128/jcm.28.3.422-425.1990.

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47

Wexler, Hannah M. "Outer‐Membrane Pore‐Forming Proteins in Gram‐Negative Anaerobic Bacteria." Clinical Infectious Diseases 35, s1 (2002): S65—S71. http://dx.doi.org/10.1086/341923.

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48

Źródłowski, Tomasz, Joanna Sobońska, Dominika Salamon, Isabel M. McFarlane, Mirosław Ziętkiewicz, and Tomasz Gosiewski. "Classical Microbiological Diagnostics of Bacteremia: Are the Negative Results Really Negative? What is the Laboratory Result Telling Us About the “Gold Standard”?" Microorganisms 8, no. 3 (2020): 346. http://dx.doi.org/10.3390/microorganisms8030346.

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Standard blood cultures require at least 24–120 h to be reported as preliminary positive. The objective of this study was to compare the reliability of Gram staining and fluorescent in-situ hybridization (FISH) for detecting bacteria in otherwise negative blood culture bottles. Ninety-six sets were taken from patients with a diagnosis of sepsis. Six incomplete blood culture sets and eight blood cultures sets demonstrating positive growth were excluded. We performed Gram stain and FISH on 82 sets taken from post-operative septic patients: 82 negative aerobic blood cultures, 82 anaerobic blood c
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Oludiran, Adenrele, David S. Courson, Malia D. Stuart, et al. "How Oxygen Availability Affects the Antimicrobial Efficacy of Host Defense Peptides: Lessons Learned from Studying the Copper-Binding Peptides Piscidins 1 and 3." International Journal of Molecular Sciences 20, no. 21 (2019): 5289. http://dx.doi.org/10.3390/ijms20215289.

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The development of new therapeutic options against Clostridioides difficile (C. difficile) infection is a critical public health concern, as the causative bacterium is highly resistant to multiple classes of antibiotics. Antimicrobial host-defense peptides (HDPs) are highly effective at simultaneously modulating the immune system function and directly killing bacteria through membrane disruption and oxidative damage. The copper-binding HDPs piscidin 1 and piscidin 3 have previously shown potent antimicrobial activity against a number of Gram-negative and Gram-positive bacterial species but hav
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Pöllänen, M. T., M. A. Laine, R. Ihalin, and V. J. Uitto. "Host-Bacteria Crosstalk at the Dentogingival Junction." International Journal of Dentistry 2012 (2012): 1–14. http://dx.doi.org/10.1155/2012/821383.

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The dentogingival junction is of crucial importance in periodontal host defense both structurally and functionally. Oral bacteria exert a constant challenge to the host cells and tissues at the dentogingival junction. The host response is set up to eliminate the pathogens by the innate and adaptive defense mechanisms. In health, the commensal bacteria and the host defense mechanisms are in a dynamic steady state. During periodontal disease progression, the dental bacterial plaque, junctional epithelium (JE), inflammatory cells, connective tissue, and bone all go through a series of changes. Th
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