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Journal articles on the topic 'Bacteriophage'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

TARAKANOV, R. I., A. N. IGNATOV, and F. S. DZHALILOV. "ISOLATION OF SPECIFIC BACTERIOPHAGES - PSEUDOMONAS SAVASTANOI PV. GLYCINEA - AND THEIR USE IN SOYBEAN BACTERIAL BLIGHT CONTROL." Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii, no. 4 (2020): 43–53. http://dx.doi.org/10.26897/0021-342x-2020-4-43-53.

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Bacterial blight is one of most harmful diseases of legumes, reducing the profitability of soybean production in Russian Federation. Among a number of Pseudomonas isolates obtained from diseased seeds and plants of soybean, 4 strains were selected and confirmed as Pseudomonas savastanoipv. glycinea (Psg). Properties of the isolated bacteria were similar to type strain of Psg CFBP 2214 in plant virulence, LOPAT tests, and PCR analysis for coronafacate ligase gene, and partly – in the phage reaction profile. Four isolates of bacteriophages specific to Psg were obtained from soil samples taken fr

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Samir, Safia. "Bacteriophages as Therapeutic Agents: Alternatives to Antibiotics." Recent Patents on Biotechnology 15, no. 1 (2021): 25–33. http://dx.doi.org/10.2174/1872208315666210121094311.

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: Bacteriophages are bacterio-specific viruses that constitute the main portion of the environment. Bacteriophages inject their genome into the targeted bacterial cells and some of them can disrupt the metabolism of bacteria and cause bacterial cell disintegration. The application of bacteriophages to kill bacteria is known as bacteriophage therapy. Since bacteriophages target bacteria and are strain-specific, every bacteriophage/bacterial host pair is unique. They are believed to cause no harm to humans. An additional advantage of the strain-specific nature of bacteriophages is that they do n

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Li, Jinyu, and John J. Dennehy. "Differential Bacteriophage Mortality on Exposure to Copper." Applied and Environmental Microbiology 77, no. 19 (2011): 6878–83. http://dx.doi.org/10.1128/aem.05661-11.

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ABSTRACTMany studies report that copper can be used to control microbial growth, including that of viruses. We determined the rates of copper-mediated inactivation for a wide range of bacteriophages. We used two methods to test the effect of copper on bacteriophage survival. One method involved placing small volumes of bacteriophage lysate on copper and stainless steel coupons. Following exposure, metal coupons were rinsed with lysogeny broth, and the resulting fluid was serially diluted and plated on agar with the corresponding bacterial host. The second method involved adding copper sulfate

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Gorshenin, Aleksandr Vladimirovich. "Participation of microbiologists Z.V. Ermolyeva and L.M. Yakobson in a scientific discussion about the fate of the production of Soviet cholera bacteriophages in 1967." Samara Journal of Science 10, no. 4 (2021): 201–7. http://dx.doi.org/10.17816/snv2021104211.

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Since the late 1920s in the USSR, along with many countries, there has been an interest in studying the phenomenon of bacteriophagy and its use in medicine. Bacteriophages are called bacterial viruses. Significant work on the study of bacteriophages and their use for medical purposes was carried out by Soviet microbiologists Zinaida Vissarionovna Ermolyeva and Lidiya Mikhailovna Yakobson. They paid especially great attention to the study of cholera bacteriophage, which during the Great Patriotic War helped prevent the cholera epidemic in frontline Stalingrad. In the 1940s due to the advent of

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Moon, Choi, Jeong, Sohn, Han, and Oh. "Research Progress of M13 Bacteriophage-Based Biosensors." Nanomaterials 9, no. 10 (2019): 1448. http://dx.doi.org/10.3390/nano9101448.

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Recently, new virus-based sensor systems that operate on M13 bacteriophage infrastructure have attracted considerable attention. These systems can detect a range of chemicals with excellent sensitivity and selectivity. Filaments consistent with M13 bacteriophages can be ordered by highly established forms of self-assembly. This allows M13 bacteriophages to build a homogeneous distribution and infiltrate the network structure of nanostructures under mild conditions. Phage display, involving the genetic engineering of M13 bacteriophages, is another strong feature of the M13 bacteriophage as a fu

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Jamaledin, Rezvan, Rossella Sartorius, Concetta Di Natale, Raffaele Vecchione, Piergiuseppe De Berardinis, and Paolo Antonio Netti. "Recombinant Filamentous Bacteriophages Encapsulated in Biodegradable Polymeric Microparticles for Stimulation of Innate and Adaptive Immune Responses." Microorganisms 8, no. 5 (2020): 650. http://dx.doi.org/10.3390/microorganisms8050650.

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Escherichia coli filamentous bacteriophages (M13, f1, or fd) have attracted tremendous attention from vaccinologists as a promising immunogenic carrier and vaccine delivery vehicle with vast possible applications in the development of vaccines. The use of fd bacteriophage as an antigen delivery system is based on a modification of bacteriophage display technology. In particular, it is designed to express multiple copies of exogenous peptides (or polypeptides) covalently linked to viral capsid proteins. This study for the first time proposes the use of microparticles (MPs) made of poly (lactic-

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Khan, Muhammad Fayaz, Aamer Ali Khattak, Afshan Saleem, Muhammad Rizwan, Muhammad Asif, and Iqbal Ahmad Alvi. "Efficient Reduction of Pseudomonas aeruginosa Biofilms Using the Myoviridae Lytic Bacteriophage vBPaeM MLG." Lahore Garrison University Journal of Life Sciences 7, no. 03 (2023): 262–79. http://dx.doi.org/10.54692/lgujls.2023.0703275.

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Pseudomonas aeruginosa biofilm infections pose significant challenges in clinical settings due to their increased resistance to conventional antibiotics. Bacteriophages, viruses that infect and kill bacteria, have emerged as promising agents for combating biofilm-related infections. This study aimed to isolate and characterize a potent bacteriophage with antibiofilm activity against P. aeruginosa. Hospital sewage was utilized to isolate a bacteriophage targeting P. aeruginosa. Quantification of phages was conducted through spot tests and doublelayer agar methods. The stability of the isolated

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SAKMANOĞLU, Aslı, та Hasan Hüseyin HADİMLİ. "Typing of ΦSP–3 lytic Salmonella bacteriophages obtained from various fecal sources". TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES 44, № 5 (2020): 1047–54. http://dx.doi.org/10.3906/vet-2005-105.

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Although several reports are available on both ΦSP–1 and ΦSP–3 lytic Salmonella bacteriophages obtained from poultry, further research is required to study the effectiveness of ΦSP–3 type on serovars isolated from other sources. In the present study, we aimed to isolate bacteriophages from 8 serovars previously obtained from 869 fecal samples (calf, dairy cow, buffalo, and camel), genotype the bacteriophages, and detect the cross-lytic activities of the bacteriophages on Salmonella enterica subsp. enterica serovar Kentucky, S.Anatum, and S.Muenchen. A total of 16 bacteriophages were detected a

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Xu, Anqi. "Draft Of Bacteriophage Therapy Review." Highlights in Science, Engineering and Technology 74 (December 29, 2023): 1105–16. http://dx.doi.org/10.54097/hfwfxj54.

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Phage therapy, which uses bacterial viruses (bacteriophages) to treat bacterial infections. In recent years, the issue of antibiotic resistance in various bacteria has become prominent, and the effectiveness of antibiotic drugs has generally declined, which has re raised people's scrutiny of bacteriophage therapy. Traditionally, bacteriophage therapy relies on the use of natural bacteriophages to infect and dissolve bacteria at the site of infection. Current research on the use of bacteriophages and their lytic proteins to combat multidrug-resistant bacterial infections suggests that bacteriop

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Kargina, T. M., E. I. Sakanyan, D. S. Davydov, and R. L. Parfenyuk. "Elaboration of Pharmacopoeial Quality Standards for Bacteriophage Products." BIOpreparations. Prevention, Diagnosis, Treatment 19, no. 4 (2019): 233–41. http://dx.doi.org/10.30895/2221-996x-2019-19-4-233-241.

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Bacteriophages are novel safe and efficacious medicinal products that are used for treatment of intestinal infections and purulent inflammations. The fact that virulent phages can be adapted to fight antibiotic-resistant bacterial strains makes this group of medicines a promising means of treatment of infections associated with medical interventions. The elaboration of quality standards for bacteriophage products will enable alignment of the quality requirements and test methods. There are no monographs on bacteriophage products in pharmacopoeias of other countries, therefore, the development

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Schrader, Holly S., John O. Schrader, Jeremy J. Walker, Thomas A. Wolf, Kenneth W. Nickerson, and Tyler A. Kokjohn. "Bacteriophage infection and multiplication occur inPseudomonas aeruginosastarved for 5 years." Canadian Journal of Microbiology 43, no. 12 (1997): 1157–63. http://dx.doi.org/10.1139/m97-164.

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Bacteriophages specific for Pseudomonas aeruginosa and Escherichia coli were examined for their ability to multiply in stationary phase hosts. Four out of five bacteriophages tested, including E. coli bacteriophage T7M, were able to multiply in stationary phase hosts. The bacteriophage ACQ had a mean burst size of approximately 1000 in exponential phase P. aeruginosa hosts and 102 in starved hosts, with corresponding latent periods that increased from 65 to 210 min. The bacteriophage UT1 had a mean burst size of approximately 211 in exponential phase P. aeruginosa hosts and 11 in starved hosts

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Florent, Perrine, Henry-Michel Cauchie, Malte Herold, Stéphan Jacquet, and Leslie Ogorzaly. "Soil pH, Calcium Content and Bacteria as Major Factors Responsible for the Distribution of the Known Fraction of the DNA Bacteriophage Populations in Soils of Luxembourg." Microorganisms 10, no. 7 (2022): 1458. http://dx.doi.org/10.3390/microorganisms10071458.

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Bacteriophages participate in soil life by influencing bacterial community structure and function, biogeochemical cycling and horizontal gene transfer. Despite their great abundance, diversity, and importance in microbial processes, they remain little explored in environmental studies. The influence of abiotic factors on the persistence of bacteriophages is now recognized; however, it has been mainly studied under experimental conditions. This study aimed to determine whether the abiotic factors well-known to influence bacteriophage persistence also control the natural distribution of the know

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Raees, Fahad, Azian Harun, Abdalla Ahmed, and Zakuan Zainy Deris. "Potential Usefulness of Bacteriophages for the Treatment of Multidrug-Resistant Acinetobacter Infection." Malaysian Journal of Medical Sciences 30, no. 5 (2023): 7–22. http://dx.doi.org/10.21315/mjms2023.30.5.2.

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Bacteriophages were discovered in early 20th century. However, the interest in bacteriophage research was reduced with the discovery of antibiotics. With the increasing number of infections due to multidrug-resistant (MDR) organisms, the potential usefulness of bacteriophages as therapeutic agents has been re-evaluated. In this review, we found that more than 30 lytic bacteriophages that infect Acinetobacter species have been characterised. These are mainly members of Caudovirales, with genome sizes ranging from 31 kb to 234 kb and G+C contents ranging from 33.5% to 45.5%. The host range can b

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Hu, Yang, Bader Al Shaaer, Lu Liang, and Ian F. Connerton. "Campylobacter Bacteriophage Infection at Refrigeration Temperatures." Applied Microbiology 3, no. 4 (2023): 1392–406. http://dx.doi.org/10.3390/applmicrobiol3040094.

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The application of bacteriophages to control foodborne bacterial pathogens in foods has gained traction in recent years. Poultry meat is a major source of Campylobacter jejuni, and a target for the application of bacteriophages. To offer the prospect of a post-harvest control measure, the bacteriophage must function at refrigeration temperatures, where C. jejuni does not grow but can survive. Here, we report actions of three classes of Campylobacter bacteriophage at 4 °C. The pre-incubation of broth cultures at 4 °C before a shift to 42 °C under conditions that support the growth of the host b

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Ariyanti, Tati. "The Use of Bacteriophage for Detection and Biocontrol of Foodborne Pathogen." Indonesian Bulletin of Animal and Veterinary Sciences 28, no. 1 (2018): 33. http://dx.doi.org/10.14334/wartazoa.v28i1.1791.

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Bacteriophages are viruses that have ability to attack bacterial cells in specific receptors, infect, multiply in bacterial cells and eventually lyse bacterial cells. This unique bacteriophage character is highly beneficial because it is harmless to mammalian cells and does not interfere with natural microbes. Bacteriophages are easy to obtain because they are widespread in the environment such as soil, water, animal, and farm waste or food. This paper describes the potential use of bacteriophages to detect pathogen and foodborne pathogen biocontrol. Bacteriophages are very potential to contro

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Manrique, Pilar, Benjamin Bolduc, Seth T. Walk, John van der Oost, Willem M. de Vos, and Mark J. Young. "Healthy human gut phageome." Proceedings of the National Academy of Sciences 113, no. 37 (2016): 10400–10405. http://dx.doi.org/10.1073/pnas.1601060113.

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The role of bacteriophages in influencing the structure and function of the healthy human gut microbiome is unknown. With few exceptions, previous studies have found a high level of heterogeneity in bacteriophages from healthy individuals. To better estimate and identify the shared phageome of humans, we analyzed a deep DNA sequence dataset of active bacteriophages and available metagenomic datasets of the gut bacteriophage community from healthy individuals. We found 23 shared bacteriophages in more than one-half of 64 healthy individuals from around the world. These shared bacteriophages wer

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Reyneke, Brandon, Benjamin Havenga, Monique Waso-Reyneke, Sehaam Khan, and Wesaal Khan. "Benefits and Challenges of Applying Bacteriophage Biocontrol in the Consumer Water Cycle." Microorganisms 12, no. 6 (2024): 1163. http://dx.doi.org/10.3390/microorganisms12061163.

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Bacteria (including disinfection- and antibiotic-resistant bacteria) are abundant in the consumer water cycle, where they may cause disease, and lead to biofouling and infrastructure damage in distributions systems, subsequently resulting in significant economic losses. Bacteriophages and their associated enzymes may then offer a biological control solution for application within the water sector. Lytic bacteriophages are of particular interest as biocontrol agents as their narrow host range can be exploited for the targeted removal of specific bacteria in a designated environment. Bacteriopha

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Putra, Ramendra Dirgantara, and Diana Lyrawati. "Interactions between Bacteriophages and Eukaryotic Cells." Scientifica 2020 (June 9, 2020): 1–8. http://dx.doi.org/10.1155/2020/3589316.

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As the name implies, bacteriophage is a bacterium-specific virus. It infects and kills the bacterial host. Bacteriophages have gained attention as alternative antimicrobial entities in the science community in the western world since the alarming rise of antibiotic resistance among microbes. Although generally considered as prokaryote-specific viruses, recent studies indicate that bacteriophages can interact with eukaryotic organisms, including humans. In the current review, these interactions are divided into two categories, i.e., indirect and direct interactions, with the involvement of bact

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Gomez, Mellissa, Alexandra Szewczyk, Jake Szamosi, Vincent Leung, Carlos Filipe, and Zeinab Hosseinidoust. "Stress Exposure of Evolved Bacteriophages under Laboratory versus Food Processing Conditions Highlights Challenges in Translatability." Viruses 15, no. 1 (2022): 113. http://dx.doi.org/10.3390/v15010113.

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Bacterial viruses, or bacteriophages, are highly potent, target-specific antimicrobials. Bacteriophages can be safely applied along the food production chain to aid control of foodborne pathogens. However, bacteriophages are often sensitive to the environments encountered in food matrices and under processing conditions, thus limiting their applicability. We sought to address this challenge by exposing commercially available Listeria monocytogenes bacteriophage, P100, to three stress conditions: desiccation, elevated temperature, and low pH, to select for stress-resistant bacteriophages. The s

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Musin, Egor V., Aleksandr L. Kim, Alexey V. Dubrovskii, Ekaterina B. Kudryashova, Elena V. Ariskina, and Sergey A. Tikhonenko. "The Influence of Polyanions and Polycations on Bacteriophage Activity." Polymers 13, no. 6 (2021): 914. http://dx.doi.org/10.3390/polym13060914.

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Phage therapy is a great alternative to antibiotic drugs, but it can’t effectively overcome the over-acidic medium of the stomach. We offer the use of polyelectrolyte microcapsules as a protective means of bacteriophage. It is necessary to understand the influence of polyelectrolytes on bacteriophage survival. The work studied the effect of polyanions and polycations on the coliprotetic bacteriophage’s viability. We have shown that polyallylamine decreased bacteriophage’s viability during increasing polyelectrolyte concentration and polyarginine had a lower inhibitory effect (then PAH) on the

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Musin, Egor V., Aleksandr L. Kim, Alexey V. Dubrovskii, Elena V. Ariskina, Ekaterina B. Kudryashova, and Sergey A. Tikhonenko. "The Pathways to Create Containers for Bacteriophage Delivery." Polymers 14, no. 3 (2022): 613. http://dx.doi.org/10.3390/polym14030613.

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Antimicrobial resistance is a global public health threat. One of the possible ways to solve this problem is phage therapy, but the instability of bacteriophages hinders the development of this approach. A bacteriophage delivery system that stabilizes the phage is one of the possible solutions to this problem. This study is dedicated to exploring methods to create encapsulated forms of bacteriophages for delivery. We studied the effect of proteolytic enzymes on the destruction of the polyelectrolyte microcapsule shell and revealed that protease from Streptomyces griseus was able to destroy the

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Levanova, L. A., Yu V. Zakharova, A. A. Markovskaya, and L. Yu Otdushkina. "Bacteriophage sensitivity of opportunistic microbiota in children with gut dysbiosis." Fundamental and Clinical Medicine 7, no. 3 (2022): 40–45. http://dx.doi.org/10.23946/2500-0764-2022-7-3-40-45.

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Aim. As the activity of bacteriophages is species- and strain-specific, it is necessary to study bacteriophage sensitivity in distinct geographic regions with various disease patterns. Here, we aimed to study the lytic activity of specific commercially available bacteriophages against Klebsiella spp., Proteus spp., and Staphylococcus aureus isolated from the intestines of children with gut dysbiosis.Materials and Methods. Bacteriophage sensitivity was assessed in 315 opportunistic microorganisms (125 Staphylococcus aureus strains, 120 Klebsiella spp. strains, 70 Proteus spp. strains) isolated

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Bach, S. J., R. P. Johnson, K. Stanford, and T. A. McAllister. "Bacteriophages reduce Escherichia coli O157:H7 levels in experimentally inoculated sheep." Canadian Journal of Animal Science 89, no. 2 (2009): 285–93. http://dx.doi.org/10.4141/cjas08083.

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Bacteriophage biocontrol has potential as a means of mitigating the prevalence of Escherichia coli O157:H7 in ruminants. The efficacy of oral administration of bacteriophages for reducing fecal shedding of E. coli O157:H7 by sheep was evaluated using 20 Canadian Arcott rams (50.0 ± 3.0) housed in four rooms (n = 5) in a contained facility. The rams had ad libitum access to drinking water and a pelleted barley-based total mixed ration, delivered once daily. Experimental treatments consisted of administration of E. coli O157:H7 (O157), E. coli O157:H7+bacteriophages (O157+phage), bacteriophages

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Kochetova, T. A., V. V. Yuskevich, F. M. Zurabov, G. T. Sadykova, P. V. Medvedev, and V. M. Popova. "Isolation of novel <i>Actinomyces oris</i> and <i>Aggregatibacter actinomycetemcomitans</i> bacteriophages and study of their biological characteristics <i>in vitro</i>." Biological Products. Prevention, Diagnosis, Treatment 24, no. 1 (2024): 103–16. http://dx.doi.org/10.30895/2221-996x-2024-24-1-103-116.

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SCIENTIFIC RELEVANCE. The incidence of periodontal infections with antibiotic-resistant strains of Actinomyces oris and Aggregatibacter actinomycetemcomitans capable of forming biofilms in the oral cavity is increasing, and the effectiveness of antimicrobials against biofilms is insufficient. Therefore, the isolation of bacteriophages active against A. oris and Ag. actinomycetemcomitans is an urgent task.AIM. This study aimed to isolate bacteriophages active against A. oris and Ag. actinomycetemcomitans, study their biological properties, and select optimum culture conditions providing pure li

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Dusza, Izabela, Dominika Jama, Grzegorz Skaradziński, Paulina Śliwka, Tomasz Janek, and Aneta Skaradzińska. "Bacteriophages Improve the Effectiveness of Rhamnolipids in Combating the Biofilm of Candida albicans." Molecules 30, no. 8 (2025): 1772. https://doi.org/10.3390/molecules30081772.

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Biofilms formed by Candida albicans pose therapeutic challenges due to their resistance to conventional antimicrobials, highlighting the need for more effective treatments. Rhamnolipids (RLs) are biosurfactants with diverse antimicrobial properties. Bacteriophages are viruses that target specific bacterial strains. Recent studies have shown that they may affect biofilm formation by fungi and yeasts. This study investigated the combined antimicrobial effects of RLs and bacteriophages against C. albicans biofilms, focusing on their anti-adhesive and inhibitory effects on biofilm development. RT-

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Abdul Choliq, Fery, Mintarto Martosudiro, Istiqomah Istiqomah, and Muhammad Fanhash Nijami. "ISOLASI DAN UJI KEMAMPUAN BAKTERIOFAG SEBAGAI AGENS PENGENDALI PENYAKIT LAYU BAKTERI (Ralstonia solanacearum) PADA TANAMAN TOMAT." VIABEL: Jurnal Ilmiah Ilmu-Ilmu Pertanian 14, no. 1 (2020): 8–20. http://dx.doi.org/10.35457/viabel.v14i1.996.

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Tomato is the top priority in the development of horticultural crops. The obstacle which mostly encountered is Ralstonia solanacearum pathogen attack. Bacteriophage can cause bacterial lysis after they they develop themselves inside the bacteria. The specific of the bacteriophage can provide result quickly, accurately, and efficiently so that it can be used as an alternative to control bacterial wilt disease R. solanacearum environmentally friendly. This study aims to find out the effectiveness of bacteriophage to control the bacterial wilt disease R. solanacearum. The testing method are plaqu

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Siringan, Patcharin, Phillippa L. Connerton, Robert J. H. Payne, and Ian F. Connerton. "Bacteriophage-Mediated Dispersal of Campylobacter jejuni Biofilms." Applied and Environmental Microbiology 77, no. 10 (2011): 3320–26. http://dx.doi.org/10.1128/aem.02704-10.

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ABSTRACTBacteria in their natural environments frequently exist as mixed surface-associated communities, protected by extracellular material, termed biofilms. Biofilms formed by the human pathogenCampylobacter jejunimay arise in the gastrointestinal tract of animals but also in water pipes and other industrial situations, leading to their possible transmission into the human food chain either directly or via farm animals. Bacteriophages are natural predators of bacteria that usually kill their prey by cell lysis and have potential application for the biocontrol and dispersal of target bacteria

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Roseline, Felicia, Diana Elizabeth Waturangi, and Yogiara Y. "ISOLATION, CHARACTERIZATION, AND APPLICATION OF BACTERIOPHAGES AGAINST SEVERAL FOOD SPOILAGE BACTERIA: Bacillus subtilis, Bacillus cereus, AND Shewanella Putrefaciens." Bacterial Empire 4, no. 2 (2021): e263. http://dx.doi.org/10.36547/be.263.

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Introduction: Bacteriophages can be the natural alternative method to prevent food spoilage caused by bacteria. This research was conducted to isolate bacteriophages from a soil sample, retail food, and wastewater from fish and then the bacteriophages will be characterized for their activity against several food spoilage bacteria, such as Bacillus cereus, Bacillus subtilis, and Shewanella putrefaciens and will be further investigated for application as food preservation. Experimental: B. cereus (Atma Jaya culture collection), B. subtilis ATCC 6633, and S. putrefaciens ATCC 8071 were used for b

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Dudina, L. G., M. A. Malkova, A. V. Chernyad’ev, S. G. Litvinets, and A. A. Byvalov. "Effect of Bacteriophages and Gentamycine on Morphology and Vesicle Formation of Bacteria Yersinia pestis EV." Problems of Particularly Dangerous Infections, no. 2 (July 3, 2019): 50–54. http://dx.doi.org/10.21055/0370-1069-2019-2-50-54.

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Objective was to assess the effect of specific bacteriophages and gentamycine on the morphological-functional properties of bacteria in the vaccine strain Yersinia pestis EV.Materials and methods. The vaccine strain Y. pestis EV, Pokrovskaya bacteriophage and the pseudotuberculous diagnostic bacteriophage were used for the study. The microbial culture was grown on solid and in liquid growth media at 27 °C for 20–24 h. The co-incubation of bacteria and bacteriophage or gentamycine was carried out at 27 °C for 20 minutes or at 37 °C for 2 hours, respectively. Culture preparations were examined b

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Grygorcewicz, Bartłomiej, Rafał Rakoczy, Marta Roszak, et al. "Rotating Magnetic Field-Assisted Reactor Enhances Mechanisms of Phage Adsorption on Bacterial Cell Surface." Current Issues in Molecular Biology 44, no. 3 (2022): 1316–25. http://dx.doi.org/10.3390/cimb44030088.

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Growing interest in bacteriophage research and use, especially as an alternative treatment option for multidrug-resistant bacterial infection, requires rapid development of production methods and strengthening of bacteriophage activities. Bacteriophage adsorption to host cells initiates the process of infection. The rotating magnetic field (RMF) is a promising biotechnological method for process intensification, especially for the intensification of micromixing and mass transfer. This study evaluates the use of RMF to enhance the infection process by influencing bacteriophage adsorption rate.

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Hu, J. Y., S. L. Ong, L. F. Song, et al. "Removal of MS2 bacteriophage using membrane technologies." Water Science and Technology 47, no. 12 (2003): 163–68. http://dx.doi.org/10.2166/wst.2003.0642.

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Removals of MS2 bacteriophage virus using different membrane materials under different operating pressures were investigated. The results obtained in this study suggested that a better log removal in terms of MS2 bacteriophage virus could be achieved using Polyamide RO membrane under the optimum operating pressure of 100 psi. It is further noted that variable MS2 influent concentration levels resulted in corresponding variable log removals of the bacteriophages by the Polyamide RO membrane. The presence of MS2 bacteriophage virus in the effluent could possibly be due to leakage of bacteriophag

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Campos, Welida F., Erica C. Silva, Thais J. Oliveira, et al. "Transdermal permeation of bacteriophage particles by choline oleate: potential for treatment of soft-tissue infections." Future Microbiology 15, no. 10 (2020): 881–96. http://dx.doi.org/10.2217/fmb-2019-0290.

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Aim: A sodium alginate-based biohydrogel was prepared integrating choline oleate deep euthetic solvent as facilitator of transdermal delivery and a cocktail of lytic bacteriophages for Acinetobacter baumannii, aiming at treating soft-tissue infections by the aforementioned pathogen. Materials & methods: Two bacteriophages were isolated from a hospital sewage and a wastewater treatment plant sewage in Sorocaba (Brazil), and characterized via SDS-PAGE electrophoresis, transmission electron microscope and evaluation of lytic spectra of the bacteriophage cocktail. The biohydrogel was prepared

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Pleška, Maroš, and Călin C. Guet. "Effects of mutations in phage restriction sites during escape from restriction–modification." Biology Letters 13, no. 12 (2017): 20170646. http://dx.doi.org/10.1098/rsbl.2017.0646.

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Restriction–modification systems are widespread genetic elements that protect bacteria from bacteriophage infections by recognizing and cleaving heterologous DNA at short, well-defined sequences called restriction sites. Bioinformatic evidence shows that restriction sites are significantly underrepresented in bacteriophage genomes, presumably because bacteriophages with fewer restriction sites are more likely to escape cleavage by restriction–modification systems. However, how mutations in restriction sites affect the likelihood of bacteriophage escape is unknown. Using the bacteriophage λ and

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Adhikari, Niran, and Krishna P. Acharya. "Effectiveness of Bacteriophage Therapy in Field Conditions and Possible Future Applications." Current Pharmaceutical Biotechnology 21, no. 5 (2020): 364–73. http://dx.doi.org/10.2174/1389201021666191217111156.

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Background: Bacteriophages are viruses, which are obligate parasites of specific bacteria for the completion of their lifecycle. Bacteriophages could be the possible alternative to antibioticresistant bacterial diseases. With this objective, extensive research in different fields is published which are discussed in this article. Results: After a review of bacteriophage therapy, bacteriophages were found to be effective against the multidrug-resistant bacteria individually or synergistically with antibiotics. They were found to be more effective, even better than the bacteria in the development

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Yarn Wai, Ga, John Yew Huat Tang, and Son Radu. "Bacteriophages as Biocontrol Agent in Food Safety." International Journal of Engineering & Technology 7, no. 4.43 (2018): 10–18. http://dx.doi.org/10.14419/ijet.v7i4.43.25810.

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Food contamination with pathogens has been continual and significant problem worldwide. These pathogens causes foodborne diseases that lead to deleterious effect to the health of consumer and the financial losses to food manufacturer. The emergence of foodborne pathogen isÂ further complicated with the infection from multiple antibiotic resistance food pathogens and the formation of biofilm on food processing equipment as well as utensils that cause the process of decontamination become more challenging. Bacteriophage is recognized for its effectiveness as biocontrol against food pathogen and

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Nishiyama, Hiroki, Hisashi Endo, Romain Blanc-Mathieu, and Hiroyuki Ogata. "Ecological Structuring of Temperate Bacteriophages in the Inflammatory Bowel Disease-Affected Gut." Microorganisms 8, no. 11 (2020): 1663. http://dx.doi.org/10.3390/microorganisms8111663.

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The aim of this study was to elucidate the ecological structure of the human gut temperate bacteriophage community and its role in inflammatory bowel disease (IBD). Temperate bacteriophages make up a large proportion of the human gut microbiota and are likely to play a role in IBD pathogenesis. However, many of these bacteriophages await characterization in reference databases. Therefore, we conducted a large-scale reconstruction of temperate bacteriophage and bacterial genomes from the whole-metagenome sequence data generated by the IBD Multi’omics Database project. By associating phages with

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Madere, Ferralita S., Michael Sohn, Angelina K. Winbush, et al. "Transkingdom Analysis of the Female Reproductive Tract Reveals Bacteriophages form Communities." Viruses 14, no. 2 (2022): 430. http://dx.doi.org/10.3390/v14020430.

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The female reproductive tract (FRT) microbiome plays a vital role in maintaining vaginal health. Viruses are key regulators of other microbial ecosystems, but little is known about how the FRT viruses (virome), particularly bacteriophages that comprise the phageome, impact FRT health and dysbiosis. We hypothesize that bacterial vaginosis (BV) is associated with altered FRT phageome diversity, transkingdom interplay, and bacteriophage discriminate taxa. Here, we conducted a retrospective, longitudinal analysis of vaginal swabs collected from 54 BV-positive and 46 BV-negative South African women

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Azizah, Nur, and Irda Safni. "In Vitro Assay of Lytic Bacteriophage to Suppress the Growth of Ralstonia syzygii subsp. indonesiensis, the Causal Pathogen of Potato Wilt Disease." Indonesian Journal of Agricultural Research 7, no. 2 (2024): 79–90. http://dx.doi.org/10.32734/injar.v7i2.14192.

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Bacterial wilt disease, the one of the major diseases of potatoes, caused by Ralstonia syzygii subsp. indonesiensis (Rsi). Many efforts have been made to control bacterial wilt disease, including physical control, chemicals, and the use of bacteriophages. Previous studies have shown that bacteriophage application in controlling plant diseases is a fast-expanding area and has great potential to replace chemical methods. This study aims to determine the potential of lytic bacteriophage in suppressing the growth of Rsi in vitro. This study used a Non-Factorial, Completely Randomized Design with 3

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Ali, Mohammed, Shurooq Kadhim, and Ahmed Abdulamir. "EXTRACTION, PURIFICATION AND THERAPEUTIC USE OF BACTERIOPHAGE ENDOLYSIN AGAINST MULTI-DRUG RESISTANT PSEUDOMONAS AERUGINOSA: AN IN-VITRO AND IN-VIVO STUDY." Iraqi Journal of Medical Sciences 16, no. 4 (2018): 405–12. http://dx.doi.org/10.22578/ijms.16.4.8.

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Background: Persistent infection with multidrug resistant Pseudomonas aeruginosa (P. aeruginosa) represents a real problem for health care providers. Bacteriophage lytic enzymes or lysins are highly evolved molecules that have been specifically developed by phages to quickly and efficiently allow their progeny to be released from the host bacterium while destructing that bacterium. Objective: Isolation of endolysin from P. aeruginosa bacteriophages, and administering them systematically in vivo lab animal and measuring their therapeutic efficacy as well as evaluation of their biosafety. Method

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Gordina, E. M., S. A. Bozhkova, and L. N. Smirnova. "Anti-bacterial and anti-biofilm activity of bacteriophages against <i>Klebsiella pneumoniae</i> and <i>Pseudomonas aeruginosa</i> isolated from orthopedic patients." Pacific Medical Journal, no. 1 (April 14, 2023): 59–63. http://dx.doi.org/10.34215/1609-1175-2023-1-59-63.

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Objective. To investigate the susceptibility of K. pneumoniae and P. aeruginosa to a polyvalent bacteriophage preparation and its effect on biofilm formation and the strain biofilms isolated from orthopedic patients.Materials and methods. The research sample included 50 clinical isolates of K. pneumoniae and 50 clinical isolates of P. aeruginosa. Identification was performed by MALDI-TOF-MS; antibiotic susceptibility was assessed in accordance with EUCAST v 21. Detection of carbapenemase genes was carried out by real-time PCR. The strain susceptibility to the bacteriophage was determined by a

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Shruti, Sinha, and Srivastava* Soumya. "Bacteriophage and Phage-Therapy: An Alternative to Antibiotics." eLifePress 1, no. 1 (2020): 21–27. https://doi.org/10.5281/zenodo.4307923.

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Bacteriophages are known for a century but their use in therapy to cure bacterial infection is still unknown. The working on bacteriophage investigation started about a century ago with their discovery by the English microbiologist Twort in 1915. The continuous growth of resistance in bacteria for various diseases and infections has caused renewing in bacteriophage therapy against bacterial infections. Lytic bacteriophages are those bacteriophages that can kill bacteria that are resistant to antibiotics by the end of their lytic cycle. Bacteriophages use proteins for the lysis of bacteria, ter

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Styles, Kathryn M., Rapee Thummeepak, Udomluk Leungtongkam, et al. "Investigating Bacteriophages Targeting the Opportunistic Pathogen Acinetobacter baumannii." Antibiotics 9, no. 4 (2020): 200. http://dx.doi.org/10.3390/antibiotics9040200.

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The multi-drug resistance of the opportunistic pathogen Acinetobacter baumannii is of growing concern, with many clinical isolates proving to be resistant to last resort as well as front line antibiotic treatments. The use of bacteriophages is an attractive alternative to controlling and treating this emerging nosocomial pathogen. In this study, we have investigated bacteriophages collected from hospital wastewater in Thailand and we have explored their activity against clinical isolates of A. baumannii. Bacteriophage vB_AbaM_PhT2 showed 28% host range against 150 multidrug resistant (MDR) iso

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Cheong, J. P. E., J. P. E. Cheong, J. D. Brooker, and J. D. Brooker. "Isolation of a virulent bacteriophage from a Propionibacterium species in the sheep rumen." Australian Journal of Agricultural Research 51, no. 1 (2000): 119. http://dx.doi.org/10.1071/ar99069.

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Propionibacterium is a facultative anaerobe associated with the rumen epithelium, the presence of which may influence the anaerobic environment through oxygen scavenging, as well as providing a source of propionate. Factors such as bacteriophages that influence Propionibacterium populations may therefore be important regulators of rumen function. This study describes the isolation and identification of a ruminal Propionibacterium bacteriophage. Sheep rumen fluid was screened for Propionibacterium species and 3 isolates were identified and characterised. One isolate, PA1, was used as an indicat

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Verbeken, Gilbert, Isabelle Huys, Jean-Paul Pirnay, et al. "Taking Bacteriophage Therapy Seriously: A Moral Argument." BioMed Research International 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/621316.

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The excessive and improper use of antibiotics has led to an increasing incidence of bacterial resistance. In Europe the yearly number of infections caused by multidrug resistant bacteria is more than 400.000, each year resulting in 25.000 attributable deaths. Few new antibiotics are in the pipeline of the pharmaceutical industry. Early in the 20th century, bacteriophages were described as entities that can control bacterial populations. Although bacteriophage therapy was developed and practiced in Europe and the former Soviet republics, the use of bacteriophages in clinical setting was neglect

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Blanc, Remy, and Abid Nasser. "Effect of effluent quality and temperature on the persistence of viruses in soil." Water Science and Technology 33, no. 10-11 (1996): 237–42. http://dx.doi.org/10.2166/wst.1996.0680.

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Survival and adsorption of pathogenic viruses in soil material can effect the extent of groundwater pollution by the application of wastewater effluents to soil. This study was conducted to determine the effect of soil composition and wastewater quality on the adsorption of pathogenic viruses (hepatitis A virus and poliovirus 1) and model bacteriophages onto soil. Moreover, the effect of temperature, soil composition and water quality on the die-off of pathogenic viruses and model bacteriophages was also studied. The effect of water quality on virus adsorption to soil was virus type dependant.

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Esmaeil, Zadeh Mohammad Reza, Yazdi Mohammad Kazem Sharifi, Zahra Rajabi, et al. "Evaluation of Specific Bacteriophage Against Salmonella infantis and Its Antibacterial Effects Compared to Ciprofloxacin in In Vitro Conditions." Journal of Advances in Medical and Biomedical Research 30, no. 139 (2022): 154–61. https://doi.org/10.30699/jambs.30.139.154.

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<strong>Abstract:</strong>    <strong>Background and Objective: </strong>Phage therapy could be used as an alternative method to antibiotic treatments. The purpose of this study was to evaluate the antibacterial activities of isolated lytic bacteriophage against ciprofloxacin-resistant strain of <em>Salmonella infanits</em> in vitro conditions. <strong>Materials and Methods: </strong>The standard strain of <em>Salmonella infantis</em>  and its specific bacteriophage was isolated by soft agar method. Phage susceptibility to heat and pH was evaluated by th

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Kharaeva, Z. F., M. Sh Mustafaev, L. Z. Blieva, E. B. Barokova, S. M. Mustafaeva, and S. A. Dyshekova. "Evaluation of sensitivity to bacteriophages of strains isolated from children with congenital malformations of the maxillofacial region." REPORTS ADYGE (CIRCASSIAN) INTERNATIONAL ACADEMY OF SCIENCES 20, no. 1 (2020): 40–45. http://dx.doi.org/10.47928/1726-9946-2020-20-1-40-45.

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Bacteriophage are used in clinical practice along with antibiotics. It is known that in many cases phage drugs are superior to other antibacterial drugs in their activity against antibiotic-resistant pathogens. Bacteriophages do not cause toxic or allergic side effects and have no contraindications.The use of bacteriophage preparations stimulates the activation of adaptive and innate immune factors, so phage therapy is particularly effective in the treatment of chronic inflammatory diseases against the background of immunosuppressive States. Bacteriophages do not interfere with the implementat

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Heringa, Spencer D., JinKyung Kim, Xiuping Jiang, M. P. Doyle, and M. C. Erickson. "Use of a Mixture of Bacteriophages for Biological Control of Salmonella enterica Strains in Compost." Applied and Environmental Microbiology 76, no. 15 (2010): 5327–32. http://dx.doi.org/10.1128/aem.00075-10.

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ABSTRACT Bacteriophages specific to Salmonella strains were isolated from sewage effluent and characterized. A five-strain bacteriophage mixture was applied to dairy manure compost inoculated with Salmonella enterica serotype Typhimurium. Bacteriophage treatment resulted in a greater than 2-log-unit reduction of Salmonella within 4 h at all moisture levels compared to the controls.

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Kang, Ilnam, Hani Jang, Hyun-Myung Oh, and Jang-Cheon Cho. "Complete Genome Sequence of Marinomonas Bacteriophage P12026." Journal of Virology 86, no. 16 (2012): 8909–10. http://dx.doi.org/10.1128/jvi.01328-12.

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Members of the genusMarinomonasin theGammaproteobacteriaare broadly distributed in marine environments where they could be infected by bacteriophages. Here we report the genome sequence of bacteriophage P12026 that can lytically infect bacterial strain IMCC12026, a member of the genusMarinomonas. To our knowledge, this is the first genome sequence of a lytic bacteriophage infecting the genusMarinomonas.

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García-Aljaro, Cristina, Xavier Muñoz-Berbel, A. Toby A. Jenkins, Anicet R. Blanch, and Francesc Xavier Muñoz. "Surface Plasmon Resonance Assay for Real-Time Monitoring of Somatic Coliphages in Wastewaters." Applied and Environmental Microbiology 74, no. 13 (2008): 4054–58. http://dx.doi.org/10.1128/aem.02806-07.

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ABSTRACT The surface plasmon resonance (SPR) technique is a well-established method for the measurement of molecules binding to surfaces and the quantification of binding constants between surface-immobilized proteins and proteins in solution. In this paper we describe an extension of the methodology to study bacteriophage-bacterium interactions. A two-channel microfluidic SPR sensor device was used to detect the presence of somatic coliphages, a group of bacteriophages that have been proposed as fecal pollution indicators in water, using their host, Escherichia coli WG5, as a target for their

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