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Dissertations / Theses on the topic 'Bacteriophages Genetics'

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Published: 4 June 2021

Last updated: 28 January 2023

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1

Dibbens, Justin Andrew. "Studies on the control of late gene transcription in coliphage 186 /." Title page, contents and summary only, 1990. http://web4.library.adelaide.edu.au/theses/09PH/09phd543.pdf.

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2

Brathwaite, Kelly Janelle. "Interactions between Campylobacters and their bacteriophages." Thesis, University of Nottingham, 2015. http://eprints.nottingham.ac.uk/28422/.

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Campylobacter jejuni is a leading cause of human bacterial enteritis worldwide. Consumption of contaminated poultry meat is considered a major source of infection. The use of virulent bacteriophages as a form of biocontrol to specifically reduce this pathogen in poultry (phage therapy) is a promising intervention that does not rely on antimicrobials and therefore circumvents the emergence of antibiotic-resistant Campylobacter strains. In order to achieve this, a better understanding of the mechanisms involved in phage-host interactions at the molecular level would assist in the development of

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3

Mmolawa, Princess Tlou. "Molecular analysis of temperate phages in Salmonella enterica serovar Typhimurium DT 64 isolated in Australia." Title page, contents and summary only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phm6855.pdf.

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Files on accompanying CD-ROM: Appendix III Phages ST64T and ST64B sequences, are in rtf format. Bibliography: leaves 279-324. System requirements for accompanying CD-ROM: IBM or compatible ; Microsoft Word or compatible to read rtf files.

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4

GARVEY, KEVIN JAMES. "DNA SEQUENCE ANALYSIS OF BACILLUS PHAGE PHI29 RIGHT EARLY REGION AND LATE GENES 14, 15 AND 16 (LYSOZYME)." Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/183839.

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The sequence of the rightmost 4,626 bp of the Bacillus phage φ29 genome is presented and analyzed. Nine large open reading frames (ORF's) have been found. Three of these ORF's are correlated with the late genes 14, 15 and 16. The remaining six ORF's are in the right early region. One of these early ORF's has been identified as gene 17 (g17), the only early gene to have been genetically mapped in this region. The remaining ORF's (16.5, 16.6, 16.7, 16.8 and 16.9) were previously unknown. The biological efficacies of some of these putative early ORF's were demonstrated using an in vitro E. coli t

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5

Goh, Shan. "Phenotypic and genotypic characterisation of bacteriophages of Clostridium difficile." University of Western Australia. Microbiology Discipline Group, 2003. http://theses.library.uwa.edu.au/adt-WU2004.0018.

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Clostridium difficile is an important hospital-acquired pathogen causing C. difficile-associated diarrhoea (CDAD) in patients exposed to antibiotics. The lack of information on bacteriophages of C. difficile, and the potential of phages as therapeutic agents for the treatment of CDAD, prompted the isolation and characterisation of phages active against clinical isolates of C. difficile in order to determine the prevalence and significance of phages of this anaerobe. Three (5.4 %) of 56 clinical C. difficile isolates induced by mitomycin C yielded dsDNA phages C2, C5, C6 and C8. The four phages

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6

Harrison, Sharon Jane. "Targeted transgenesis and the 186 site-specific recombination system /." Title page, summary and contents only, 1999. http://web4.library.adelaide.edu.au/theses/09PH/09phh322.pdf.

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7

Huen, Shing-yan Michael, and 禤承恩. "A mechanistic study of lambdaphage-mediated recombination in E. coli." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B35321854.

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8

Swanson, Rhett. "Cloning and expression of the genes encoding bacteriophage T7 & SP6 RNA polymerase /." Title page, table of contents and summary only, 1990. http://web4.library.adelaide.edu.au/theses/09PH/09phs9722.pdf.

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9

Chang, Jenny Ren-Jye. "Scaffolding-mediated capsid size determination in bacteriophages." Birmingham, Ala. : University of Alabama at Birmingham, 2009. https://www.mhsl.uab.edu/dt/2009p/changj.pdf.

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Thesis (Ph. D.)--University of Alabama at Birmingham, 2009.<br>Title from PDF title page (viewed Jan. 26, 2010). Additional advisors: Asim K. Bej, Gail E. Christie, Peter E. Prevelige, Jr., R. Douglas Watson. Includes bibliographical references.

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10

Brumby, Anthony Mansfield. "The control of prophage induction in coliphage 186 /." Title page, contents and summary only, 1994. http://web4.library.adelaide.edu.au/theses/09PH/09phb893.pdf.

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11

Seo, Sang Beom. "The isolation and characterization of mutations in the deoxyguanosine triphosphate triphosphohydrolase (dgt) gene of ESCHERICHIA COLI." Thesis, Georgia Institute of Technology, 1990. http://hdl.handle.net/1853/25334.

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12

Gerendasy, Dan Douglas. "The genomic organization and right early transcription of bacteriophage PRD1." Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/184884.

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The bacteriophage PRD1 is a lipid bearing phage that infects a wide variety of gram-negative bacteria, including Escherichia coli and Salmonella typhimurium when they harbor the appropriate plasmid. It contains a linear duplex DNA molecule that is covalently bound by its 5' ends to a terminal protein. Like adenovirus and the Bacillus phage φ29, PRD1 specifies its own DNA polymerase which is able to utilize the phage encoded terminal protein to prime DNA synthesis. In addition to these two proteins, PRD1 also specifies an additional replication protein (p12) of unknown function. We have sequenc

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13

Fehrsen, Jeanni. "Isolation of antigenic peptides of Cowdria ruminantium and their encoding genes using a genome-derived phage display library." Thesis, Rhodes University, 2003. http://hdl.handle.net/10962/d1003979.

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The development of new and effective vaccines and immunodiagnostic reagents requires the characterisation of antigenically relevant proteins and their interactions with the products of the immune system. Phage display technology was investigated as a means of elucidating some of the antigenic properties of the rickettsial parasite, Cowdria ruminantium (Cowdria). Randomly fragmented gene-derived libraries have been useful in elucidating viral and other epitopes, but only limited work has been done with entire genomes. A phage display library expressing a repertoire of Cowdria peptides was const

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14

Huen, Shing-yan Michael. "A mechanistic study of lambdaphage-mediated recombination in E. coli." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B35321854.

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15

Kalionis, Bill. "The early control region of temperate coliphage 186 : sequence and transcription studies /." Title page, contents and summary only, 1985. http://web4.library.adelaide.edu.au/theses/09PH/09phk14.pdf.

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16

Hsieh, Jui-Cheng. "Structure-function analysis of the bacteriophage PRD1 DNA terminal protein: Nucleotide sequence, overexpression, and site-directed mutagenesis of the terminal protein gene." Diss., The University of Arizona, 1990. http://hdl.handle.net/10150/184974.

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The nucleotide sequence of the PRD1 terminal protein gene has been determined. The coding region for PRD1 terminal protein is 777 base pairs long and encodes 259 amino acid residues (29,326 daltons). The deduced amino acid sequence of PRD1 terminal protein reveals no overall homology with other known terminal proteins or related proteins. A closer examination revealed a highly conserved amino acid sequence, YSRLRT, exist among all identified DNA terminal proteins including PRD1, PZA, Nf, φ29 and adenovirus. This is the first conserved amino acid sequence that has been found in all identified D

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17

Hallewell, Jennyka, and University of Lethbridge Faculty of Arts and Science. "Shiga toxin-producing bacteriophage in Escherichia coli O157:H7." Thesis, Lethbridge, Alta. : University of Lethbridge, Deptartment of Biochemistry, 2008, 2008. http://hdl.handle.net/10133/776.

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Shiga toxin-producing E. coli (STEC) including E. coli O157:H7 are potential food and water borne zoonotic bacterial pathogens capable of causing outbreaks of severe illness in humans. The virulence of E. coli O157:H7 strains may be related to the type of Stx produced and several Stx2 variants have been identified which appear to differ in their ability to cause disease. Two lineages exist within O157 strains where lineage I is associated mainly with human and bovine isolates and lineage II is associated mainly with bovine isolates. The goal of this study was to identify and characterize a lin

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18

Hsu, Yu-Hung. "Characterization of Mannheimia haemolytica-specific bacteriophages." Thesis, Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2011, 2011. http://hdl.handle.net/10133/3150.

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Mannheimia haemolytica is the principal bacterial agent associated with bovine respiratory disease (BRD). It has a significant economic impact on the beef feedlot industry. The current methods for BRD prevention and treatment have various problems and limitations, especially with reports of increased antimicrobial resistance in M. haemolytica. Bacteriophage therapy presents a novel method to mitigate M. haemolytica. This study aimed to isolate strictly lytic M. haemolytica-specific bacteriophages from bovine nasopharyngeal swabs and feedlot trough water. This was accompanied by an extensive ch

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19

Kunapuli, Phani Chandrika. "Analysis of the Clear Plaque Phenotype of the Bacteriophage HK75." TopSCHOLAR®, 2010. http://digitalcommons.wku.edu/theses/219.

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The growth of bacteriophage HK75 is inhibited by specific mutations in the zinc binding domain of the host RNA polymerase beta prime subunit. It shares this rare property with bacteriophage HK022 and other phages that use RNA mediated antitermination to promote early gene expression. Recent genomic analysis of HK75 and HK022 has confirmed the relatedness of these two phages and place HK75 in the lambdoid family of bacteriophages. Lambdoid phages are temperate and can adopt a lytic or lysogenic lifestyle upon infection of a suitable host. However, HK75 only forms clear plaques and thus appears

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20

Iyer, Kartik. "Interaction of bacteriophage mu middle transcription activator protein mor with promoter DNA." View the abstract Download the full-text PDF version (on campus access only), 2008. http://etd.utmem.edu/ABSTRACTS/2008-033-Iyer-index.html.

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Thesis (M.S.)--University of Tennessee Health Science Center, 2008.<br>Title from title page screen (viewed on July 31, 2008). Research advisor: Martha M Howe, Ph.D. Document formatted into pages (vii, 127 p. : ill.). Vita. Abstract. Includes bibliographical references (p. 103-116).

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21

Lima, Mendez Gipsi. "Towards in silico detection and classification of prokaryotic Mobile Genetic Elements." Doctoral thesis, Universite Libre de Bruxelles, 2008. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210578.

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Bacteriophage genomes show pervasive mosaicism, indicating that horizontal gene exchange plays a crucial role in their evolution. Phage genomes represent unique combinations of modules, each of them with a different phylogenetic history. Thus, a web-like, rather than a hierarchical scheme is needed for an appropriate representation of phage evolutionary relationships. Part of the virology community has long recognized this fact and calls for changing the traditional taxonomy that classifies tailed phages according to the type of genetic materials and phage tail and head/capsid morphologies. Mo

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22

Jonnalagadda, Madhuri. "Site Directed Mutagensis of Bacteriophage HK639 and Identification of Its Integration Site." TopSCHOLAR®, 2008. http://digitalcommons.wku.edu/theses/42.

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Bacteriophages affect bacterial evolution, pathogenesis and global nutrient cycling. They are also the most numerous and diverse group of biological entities on the planet [1, 2, 3, 4, 5, 6]. Members of the Lambda phage family share a similar genetic organization and control early gene expression by suppressing transcription, a process known as antitermination. Transcription antitermination in Lambda is mediated by a phage-encoded protein whereas in lambdoid phage HK022, antitermination is mediated by a phage-encoded RNA molecules. Recent results suggest that another bacteriophage called H

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23

Mavris, Maria. "Bacteriophage SfII mediated serotype conversion in Shigella flexneri /." Title page, abstract and contents only, 1998. http://web4.library.adelaide.edu.au/theses/09PH/09phm4608.pdf.

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24

Cramer, Todd James Lucas. "Genetic mosaicism between the bacteriophage [phi]80 and bacteriophage [lambda]." Bowling Green, Ohio : Bowling Green State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=bgsu1223514067.

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25

Povinelli, Christine Marie. "Genetic analysis of the dihydrofolate reductase and thymidylate synthase genes of bacteriophage T4." Diss., Georgia Institute of Technology, 1987. http://hdl.handle.net/1853/25347.

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26

Lee, Se Il. "Statistical thermodynamics of virus assembly." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/33900.

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Experiments show that MgSO4 salt has a non-monotonic effect as a function of MgSO4 concentration on the ejection of DNA from bacteriophage lambda. There is a concentration, N0, at which the minimum amount of DNA is ejected. At lower or higher concentrations, more DNA is ejected. We propose that this non-monotonic behavior is due to the overcharging of DNA at high concentration of Mg⁺² counterions. As the Mg⁺² concentration increases from zero, the net charge of ejected DNA changes its sign from negative to positive. N0 corresponds to the concentration at which DNA is neutral. Our theory fits

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27

Cramer, Todd James. "Genetic Mosaicism Between The Bacteriophage φ80 And Bacteriophage λ". Bowling Green State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1223514067.

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28

Eriksson, Jesper. "Structure-Function Studies of Bacteriophage P2 Integrase and Cox protein." Doctoral thesis, Stockholm University, Department of Genetics, Microbiology and Toxicology, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-683.

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<p>Probably no group of organisms has been as important as bacteriophages when it comes to the understanding of fundamental biological processes like transcriptional control, DNA replication, site-specific recombination, e.t.c.</p><p>The work presented in this thesis is a contribution towards the complete understanding of these organisms. Two proteins, integrase, and Cox, which are important for the choice of the life mode of bacteriophage P2, are investigated. P2 is a temperate phage, i.e. it can either insert its DNA into the host chromosome (by site-specific recombination) and wait (lysogen

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29

Wright, Alice Ann. "The Genomic Sequence and Annotation of Bacteriophage HK239." TopSCHOLAR®, 2010. http://digitalcommons.wku.edu/theses/208.

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Bacteriophages are viruses that infect bacteria and they are the most numerous biological entities on Earth. Temperate phage can adopt two different lifestyles. In the lytic lifestyle, a phage injects its genome into the host and a controlled developmental program ensues. The phage DNA is replicated, phage genes are expressed and new viral particles are assembled. Ultimately, the host cell lyses and the phage particles are released into the environment. In the lysogenic lifestyle, a phage integrates its genome into the host chromosome, creating a prophage. The cell containing the prophage is k

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30

Long, Graham Stanley. "Molecular cloning of bacteriophage K1E endosialidase." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339539.

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31

Arap, Marco Antonio. "Estudo da proteína de choque térmico GRP78 para o desenvolvimento de um sistema de receptor-ligante para o câncer de próstata." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/5/5153/tde-31052007-122749/.

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Introdução: Apesar dos avanços nas técnicas de diagnóstico e tratamento, o câncer de próstata avançado ainda é uma condição letal. Terapêuticas mais eficazes são necessárias para reduzir as taxas de morbi-mortalidade associadas à doença. A Proteína-78 regulada pela glicose (GRP78), uma proteína de choque térmico envolvida na apresentação de antígenos, foi recentemente descrita como sendo um possível marcador molecular para o câncer de próstata. Ainda mais, a resposta imune a essa proteína mostrou correlação com o desenvolvimento de doença hormônio-independente e com pior sobrevida para a doenç

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32

Poon, Pui-wah Alice. "Genetical study of HK253 and related temperate coliphages /." [Hong Kong : University of Hong Kong], 1988. http://sunzi.lib.hku.hk/hkuto/record.jsp?B12358393.

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33

OBRINGER, JOHN WILLIAM. "GENETIC EXCLUSION IN BACTERIOPHAGE-T4 (EXONUCLEASES)." Diss., The University of Arizona, 1987. http://hdl.handle.net/10150/184090.

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Genetic exclusion in phage T4 is the prime responsibility of the imm and sp genes. The map region containing imm does not allow sufficient bps to encode for proteins the size reported for the imm gp. After assaying 30 mutants of the genes adjacent to imm, I found 7 in gene 42 that were defective in the imm phenotype. Upon reverting amNG411(42), the mutant most defective exclusion, for its gene 42 phenotype the exclusion phenotype also changed. When assayed in UGA suppressor hosts, imm+ phage showed a decreased exclusion ability indicating that an opal codon was involved in production of the fu

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34

Short, Nicholas J. "The DNA sequence of the filamentous bacteriophage Pf1." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305822.

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35

Deyoung, Katherine Leigh. "Genetic studies of self-splicing RNAs in bacteriophage T4." Diss., Georgia Institute of Technology, 1993. http://hdl.handle.net/1853/25434.

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36

潘佩華 and Pui-wah Alice Poon. "Genetical study of HK253 and related temperate coliphages." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1988. http://hub.hku.hk/bib/B31231329.

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37

Richardson, Helena Elizabeth. "Defining the early lythic region of coliphage 186 and the control of middle gene transcription /." Title page, contents and summary only, 1987. http://web4.library.adelaide.edu.au/theses/09PH/09phr522.pdf.

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38

Forghani, Farnaz. "Protein engineering of bacteriophage Mu transposase." Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=60444.

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Bacteriophage Mu is an ideal system to study DNA transposition. The 70-KDa protein product of the phage early gene A, termed transposase, is absolutely required for transposition. Transposase binds specifically at sites located at both ends of the phage genome, termed attL and attR, and at an enhancer-like element at the left end of the genome, called IAS (internal activation sequence). It then nicks at these ends, and nicks a random target DNA sequence in a 5 base pair staggered fashion with 5$ sp prime$ extensions and promotes strand transfer between the Mu ends and the target DNA. The trans

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39

Hyman, Paul Lawrence. "The genetics of bacteriophage T4 DNA repair during infection." Diss., The University of Arizona, 1991. http://hdl.handle.net/10150/185380.

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Recombinational repair is a widespread mechanism for dealing with DNA damage. It is found in both prokaryotes and eukaryotes which implies that it is an ancient process which arose early in the evolutionary history of life on Earth. In addition, it has been implicated as a driving force in the evolution of sexual reproduction. In this dissertation I report experimental results which clarify the role of recombinational repair in bacteriophage (phage) T4. The Luria-Latarjet effect is an increase in resistance to DNA damage by phage T4 during infection. It has often been assumed to involve recomb

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40

Talbot, Simon John. "Structural studies of RNA-protein interactions in the bacteriophage MS2." Thesis, University of Leeds, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303328.

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41

Davison, P. J. "In vitro packaging and recombination of DNA in bacteriophage T1." Thesis, University of Liverpool, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370842.

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42

Olubuyide, Temitope Kehinde. "Investigation of the MutT2 gene." Thesis, Georgia Institute of Technology, 1999. http://hdl.handle.net/1853/31013.

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43

Sinclair, R. B. "The repressor (c) gene of Streptomyces phage #PHI#c31." Thesis, University of East Anglia, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378897.

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44

Reed, Patricia. "Function of bacteriophage Orf recombinases in genetic exchange." Thesis, Durham University, 2006. http://etheses.dur.ac.uk/4917/.

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Recombination events in bacteriophages frequently occur by illegitimate exchange at short tracts of sequence homology, enabling these viruses to acquire novel genes and serve as vehicles for horizontal gene transfer. The emergence of new pathogenic organisms due to the acquisition of virulence determinants from bacterial viruses has stimulated considerable interest in the mechanisms of phage recombination. Bacteriophage λ encodes its own recombination system, consisting of Exo, β and γ proteins. An additional λ recombinase, Orf, participates in the early stages of exchange, supplying a functio

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45

Whichard, Jean Marie. "Bacteriophage Felix O1: Genetic Characterization and Bioremedial Application." Diss., Virginia Tech, 2000. http://hdl.handle.net/10919/29591.

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Bacteriophage Felix O1 was studied for applicability as a Salmonella intervention. Felix O1's potential as a Salmonella therapeutic was explored, as was its utility as a food application. Felix O1 is specific for and infects most serovars within the genus Salmonella. The entire 86.155-kb sequence of the phage's linear, double-stranded chromosome was determined. 213 open reading frames (ORFs) were found, including 23 homologues of phage genes (e<0.008). Homology searches do not indicate genes that would be expected to increase virulence of Salmonella. Thirteen T4 homologues were found, in

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46

com, shawnseet@gmail, and Shawn Ginn Ming Seet. "Genome sequence of bacteriophage ÖAR29 : a basis for integrative plasmid vectors." Murdoch University, 2005. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20060615.135718.

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The initial aim of this project was to characterise the integrative recombination mechanism of bacteriophage ÖAR29 , to provide a better understanding for development of the shuttle plasmid pBA as a site-specific Bacteroides integration vector. RT-PCR showed that the previously identified ÖAR29 recombination genes, integrase (Int) and excisionase (Xis), were transcribed from pBA in E. coli SCS110, B. thetaiotaomicron AR29 and B. uniformis AR20. In silico derived amino acid sequences from both genes showed only very low levels of similarity to other known Int and Xis in GenBank. To improve unde

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47

Seet, Shawn Ginn Ming. "Genome sequence of bacteriophage €AR29 : a basis for integrative plasmid vectors /." Access via Murdoch University Digital Theses Project, 2005. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20060615.135718.

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48

Amin, M. K. A. "The ecology and genetics of Pseudomonas bacteriophage in freshwater systems." Thesis, Cardiff University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381224.

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49

Petty, Nicola Karen. "New bacteriophages for two animal pathogens : tools for genetic manipulation." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.612529.

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50

Garforth, Scott John. "Structure-specific DNA cleavage and binding by bacteriophage T5 5'-3' exonuclease." Thesis, University of Sheffield, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287351.

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