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Dissertations / Theses on the topic 'Banana bunchy top virus (BBTV)'

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Published: 11 December 2022

Last updated: 31 July 2025

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1

Horser, Cathryn Louise. "Characterisation of the putative satellite DNAs associated with banana bunchy top virus." Thesis, Queensland University of Technology, 2000.

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2

Herrera, Valencia Virginia Aurora. "Molecular characterisation of the intergenic regions of banana bunchy top virus." Queensland University of Technology, 2006. http://eprints.qut.edu.au/16216/.

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Banana bunchy top virus (BBTV) is a circular, single-stranded (css) DNA virus that belongs to the genus Babuvirus in the family Nanoviridae. BBTV is responsible for the most devastating virus disease of banana known as "bunchy top", for which conventional control measures are generally ineffective. Genetically engineered resistance appears to be the most promising strategy to generate BBTV-resistant bananas but the success of this strategy is largely dependent upon the molecular characterisation of the target virus and knowledge of the virus life cycle, particularly the replication strategy. T

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3

Herrera-Valencia, Virginia Aurora. "Molecular characterisation of the intergenic regions of banana bunchy top virus." Thesis, Queensland University of Technology, 2006. https://eprints.qut.edu.au/16216/1/Virginia_Herrera-Valencia_Thesis.pdf.

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Banana bunchy top virus (BBTV) is a circular, single-stranded (css) DNA virus that belongs to the genus Babuvirus in the family Nanoviridae. BBTV is responsible for the most devastating virus disease of banana known as "bunchy top", for which conventional control measures are generally ineffective. Genetically engineered resistance appears to be the most promising strategy to generate BBTV-resistant bananas but the success of this strategy is largely dependent upon the molecular characterisation of the target virus and knowledge of the virus life cycle, particularly the replication strategy. T

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4

Tsao, Theresa Tsun-Hui. "Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication." Thesis, Queensland University of Technology, 2008. https://eprints.qut.edu.au/17031/1/Theresa_Tsao_Thesis.pdf.

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Banana bunchy top virus (BBTV) causes one of the most devastating diseases of banana. Transgenic virus resistance is now considered one of the most promising strategies to control BBTV. Pathogen-derived resistance (PDR) strategies have been applied successfully to generate plants that are resistant to numerous different viruses, primarily against those viruses with RNA genomes. BBTV is a circular, single-stranded (css) DNA virus of the family Nanoviridae, which is closely related to the family Geminiviridae. Although there are some successful examples of PDR against geminiviruses, PDR against

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5

Tsao, Theresa Tsun-Hui. "Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication." Queensland University of Technology, 2008. http://eprints.qut.edu.au/17031/.

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Banana bunchy top virus (BBTV) causes one of the most devastating diseases of banana. Transgenic virus resistance is now considered one of the most promising strategies to control BBTV. Pathogen-derived resistance (PDR) strategies have been applied successfully to generate plants that are resistant to numerous different viruses, primarily against those viruses with RNA genomes. BBTV is a circular, single-stranded (css) DNA virus of the family Nanoviridae, which is closely related to the family Geminiviridae. Although there are some successful examples of PDR against geminiviruses, PDR against

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6

Bolton, Clair Louise. "Development of a Rep-inducible, BBTV-based expression system in banana." Thesis, Queensland University of Technology, 2009. https://eprints.qut.edu.au/25939/1/Clair_Bolton_Thesis.pdf.

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Banana bunchy top is regarded as the most important viral disease of banana, causing significant yield losses worldwide. The disease is caused by Banana bunchy top virus (BBTV), which is a circular ssDNA virus belonging to the genus Babuvirus in the family Nanoviridae. There are currently few effective control strategies for this and other ssDNA viruses. “In Plant Activation” (InPAct) is a novel technology being developed at QUT for ssDNA virus-activated suicide gene expression. The technology exploits the rolling circle replication mechanism of ssDNA viruses and is based on a unique “spli

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7

Bolton, Clair Louise. "Development of a Rep-inducible, BBTV-based expression system in banana." Queensland University of Technology, 2009. http://eprints.qut.edu.au/25939/.

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Banana bunchy top is regarded as the most important viral disease of banana, causing significant yield losses worldwide. The disease is caused by Banana bunchy top virus (BBTV), which is a circular ssDNA virus belonging to the genus Babuvirus in the family Nanoviridae. There are currently few effective control strategies for this and other ssDNA viruses. “In Plant Activation” (InPAct) is a novel technology being developed at QUT for ssDNA virus-activated suicide gene expression. The technology exploits the rolling circle replication mechanism of ssDNA viruses and is based on a unique “spli

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8

Hastie, Marcus Lachlan. "In vitro activities of BBTV-REP." Thesis, Queensland University of Technology, 2001. https://eprints.qut.edu.au/37077/1/37077_Digitised%20Thesis.pdf.

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9

Wanitchakorn, Raktham. "Gene functions in banana bunchy top virus." Thesis, Queensland University of Technology, 1999.

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10

Burns, Thomas Michael. "Genome organisation of banana bunchy top virus." Thesis, Queensland University of Technology, 1994.

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11

Hafner, Gregory. "Replication of banana bunchy top virus : mechanisms and interference." Thesis, Queensland University of Technology, 1998.

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12

Beetham, Peter Ronald. "Transcription of mRNAs from the banana bunchy top virus genome." Thesis, Queensland University of Technology, 1998.

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13

Karan, Mirko. "Sequence diversity of DNA components associated with banana bunchy top virus." Thesis, Queensland University of Technology, 1995.

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14

Mware, Benard Ouma. "Development of banana bunchy top virus resistance in bananas: RNAi approach." Thesis, Queensland University of Technology, 2016. https://eprints.qut.edu.au/95736/1/Benard%20Ouma_Mware_Thesis.pdf.

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Bunchy top, caused by banana bunchy top virus (BBTV), is the most important viral disease affecting banana worldwide, for which control is very difficult. In this thesis, a molecular approach was used to generate genetically modified banana plants, some of which were resistant to BBTV infection. Further, sources of natural resistance to BBTV were identified in a range of different banana cultivars which may be exploited in future control strategies. The results from this study provide a strong platform for the future development of BBTV-resistant banana plants.

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15

Dugdale, Benjamin. "Promoter activity associated with the intergenic regions of banana bunchy top virus." Thesis, Queensland University of Technology, 1999. https://eprints.qut.edu.au/37001/6/37001_Digitised_Thesis.pdf.

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16

Soko, Misheck Mica Mafeni. "Evaluation of transgenic RNAi banana and plantain lines for resistance to banana bunchy top disease." Thesis, Queensland University of Technology, 2022. https://eprints.qut.edu.au/228515/1/Misheck%20Mica%20Mafeni_Soko_Thesis.pdf.

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This project evaluated genetically modified Cavendish bananas and plantains for field and glasshouse resistance to banana bunchy top virus in Malawi in Africa, over a three-and-a-half-year period. The study identified several GM lines with significant resistance to the virus and provided a rare insight into virus-vector relations and the climate. The research showed that rigorous field assessment of GM plants for disease resistance is critical and that immunity to this virus will likely only be achieved using a multi-faceted resistance strategy.

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17

Becker, Douglas Kenneth. "The transformation of banana with potential virus resistance genes." Thesis, Queensland University of Technology, 1999. https://eprints.qut.edu.au/37023/6/37023_Digitised_Thesis.pdf.

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One approach to reducing the yield losses caused by banana viral diseases is the use of genetic engineering and pathogen-derived resistance strategies to generate resistant cultivars. The development of transgenic virus resistance requires an efficient banana transformation method, particularly for commercially important 'Cavendish' type cultivars such as 'Grand Nain'. Prior to this study, only two examples of the stable transformation of banana had been reported, both of which demonstrated the principle of transformation but did not characterise transgenic plants in terms of the efficiency at

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18

Kato, Maiko. "Molecular strategies for resistance to circular single-stranded DNA viruses." Thesis, Queensland University of Technology, 2019. https://eprints.qut.edu.au/134475/1/Maiko_Kato_Thesis.pdf.

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This thesis describes how the "In Plant Activation" (INPACT) expression platform was adapted into a molecular resistance strategy against circular single-stranded DNA viruses. The system was first optimized using a model geminivirus in the dicot host tobacco then applied to a commercially relevant pathosystem. When used in conjunction with RNAi, the platform provided immunity to the devastating bunchy top virus in the elite banana cultivar "Cavendish". This work shows that a multi-faceted approach to engineered resistance may be a simple means of generating immunity to this important family of

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19

Kleidon, Jennifer. "Development of an excisable selectable marker system for banana." Thesis, Queensland University of Technology, 2019. https://eprints.qut.edu.au/134478/1/Jennifer_Kleidon_Thesis.pdf.

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This study describes the development of a recombinase-based platform for the removal of selectable marker genes from transgenic bananas. Using a steroid-inducible recombinase enzyme and dual selection vector containing the green fluorescent protein reporter gene, a protocol based on Agrobacterium-mediated transformation of Cavendish banana embryogenic cells was established. The system was then practically applied to generate the first ever marker-free banana plants with potential resistance to banana bunchy top virus. This platform will provide an effective means of improving this economically

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20

Hermann, Scott Richard. "Transgene expression in banana." Thesis, Queensland University of Technology, 2001.

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21

Yeh, Hsin-Hung, and 葉信宏. "Cloning and Molecular analysis of Banana bunchy top virus." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/29210634628985811488.

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碩士<br>國立臺灣大學<br>植物病蟲害學系<br>81<br>Large quantity of double and single-stranded（ds and ss） viral DNAs have been purified by using an improved purification method .Compared with viral genome（ss DNA） and hybridization analysis ,it is proved that the ss DNA is the viral DNAs contain both linear and circular form. Two ds viral DNAs have been cloned. Sequencing analysis of the ds DNA clones revealed that the viral ds DNA is circular with a size of 1094 bp. The sequence is part- ialy homolo

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22

Liu, Yu-Tzu, and 劉于滋. "Genome characterization of strains of Banana bunchy top virus." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/33816872750366187490.

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23

Anhalt, Mandy D. "Transmission characteristics of banana bunchy top virus to banana Pentalonia nigronervosa Coq." Thesis, 2007. http://hdl.handle.net/10125/20596.

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24

Chang, Hsin-Yuan, and 張馨元. "The methylation analysis and comparison between Banana bunchy top virus and Banana streak Mysore virus." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/31029277439922695005.

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碩士<br>國立臺灣大學<br>植物病理與微生物學研究所<br>99<br>Banana bunchy top virus (BBTV) and Banana streak Mysore virus (BSMysV) are DNA viruses that causing Banana bunchy top disease and Banana streak virus disease in bananas (Musa spp.), respectively. Generally, BSMysV presents as a free virus, an episome, in hosts. However, BSMysV has integrated into the genome of M. balbisiana, thus, many banana cultivars contain non-infectious BSMysV (eBSMysV). Endogenous pararetrovirus sequences may be activated to become infectious due to stress. BSMysV have an impact on the banana production because of not only its episom

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25

WU, RUI-YU, and 吳瑞鈺. "Characterization and monoclonal antibodies of the virus causing banana bunchy-top." Thesis, 1987. http://ndltd.ncl.edu.tw/handle/86755465601508651020.

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26

Liu, hsiang-cheng, and 劉向正. "Strains of Banana Bunchy-Top Virus and Transmission Natures of Vector Aphids." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/43800996693926666404.

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碩士<br>國立臺灣大學<br>植物病理學研究所<br>88<br>Single pure isolates of banana bunchy top virus (BBTV) strains were obtained through transmission by vector aphid( Pentalonia nigronervosa ). The BBTV was differentiated into such four strains as severe (S), intermediate (I), mild (M) and latent (L) strains according to symptom expression on Cavendish banana in Taiwan.S strain induced severe typical symptoms including stunted, chlorotic margin and narrow, erect new leaves, bunchy top, vein clearing and dark green dot-dash streaks on petioles. I strain caused mild stunt, slight leaf atrophy and distinct vein c

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27

Yang, Wen-Wen, and 楊文雯. "Studies on genetic transformation of banana for resistance to banana bunchy top virus using Agrobacterium tumefaciens." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/23987718051650668837.

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碩士<br>國立臺灣大學<br>園藝學研究所<br>90<br>The development of a regeneration system from banana (Musa spp. cv. ‘Pei Chiao’, Cavendish, AAA group) hand primordia on male flowers is described. The most important factors affecting embryogenic callus initiation were the source of explant and the composition of the culture medium. The closer the hand primordia is situated to the inflorescence apical meristem, the stronger is the regenerative capability. Improved callus initiation was obtained on culture medium supplemented with 1 mg/l picloram and 4 mg/l 2,4-D. Somatic embryogenesis was observed on callus cul

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28

Tsao, Li-Yu, and 曹麗玉. "Biologiacl and molecular characterization of banana bunchy top virus strains and their ecology." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/90069743342335214354.

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博士<br>國立臺灣大學<br>植物病蟲害學系<br>86<br>The banana bunchy top virus (BBTV) was differentiated into such four strains as severe (S), intermediate (I), mild (M) and latent (L) strains according to symptom expression in Taiwan. S strain induced severe typical symptoms including dwarfing, slender atrophy leaf with marginal chlorosis, bunchy top, vein clearing and green streaks on petioles. I strain caused mild stunt, slight leaf atrophy and distinct vein clearing. M strain- infected banana plants prod

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29

Shen, Tang-Long, and 沈湯龍. "Detection of banana bunchy top virus using cloned DNA probes and PCR technique." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/34307304823480160273.

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碩士<br>國立臺灣大學<br>植物病蟲害學系<br>82<br>Recently,three different BBTV-DNA components had been cloned and sequenced. The three cloned BBTV-DNA components labeled with biotin were used as DNA probes (Probe C-1, Probe C-2, and Probe C-3) for disease diagnosis. Four pair of oligonucleotides (c-1a/a-1b,c-2a/c-2b,c-3a/c-3b,and s6a/s6b) sythesized according to their sequences,were used as primer pairs in polymerase chain reactions(PCR).Using these BBTV- specific DNA probes in dot hybri dizations,po

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30

Chen, Jun Hong, and 陳俊宏. "Genomic components of banana bunchy-top virus and genomic analysis of different sympton-type strains." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/00104250278872462552.

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31

Huang, Yi-Chi, and 黃奕錡. "Biological and molecular characterization of additional replicase encoded components associated with Banana bunchy top virus." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/85788306315155538658.

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碩士<br>臺灣大學<br>植物病理與微生物學研究所<br>95<br>Banana is one of the most important fruit crops around the world. Banana bunchy top disease caused by Banana bunchy top virus (BBTV) is a destructive disease for banana. BBTV is isometric and belongs to the genus Babuvirus, in the family Nanoviridae. BBTV consists of six circular single-stranded integral DNA components, DNA 1-6. In some isolates, however, in additional to the integral components other replication competent components (additional Reps) are also associated with BBTV infection. Currently, 8 different additional Reps, Y, W1, W2, W3, W4, S1, S2,

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32

Liu, Chien-wei, and 劉建瑋. "Preparation of antibodies against bacteria expressed coat protein of Banana bunchy top virus and their application on virus detection." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/07642338410126209071.

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碩士<br>朝陽科技大學<br>生物技術研究所<br>93<br>Banana bunchy top virus (BBTV) is one of the most devastating banana diseases (Musa spp.) around the world. Its genome consists of single-stranded DNA and it is transmitted in a persistent manner by vector aphid (Pentalonia nigronervosa). The symptoms induced by BBTV include chlorotic margin, narrow erect and witches’ broom-like leaves. The infected plants are usually evidently stunted and their yield and quality of banana fruits are significantly affected. Unlike the conventional means of propagation by vegetative suckers, bananas are presently propagated

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33

Su, Yi-Rong, and 蘇怡蓉. "Studies on the expression of Banana bunchy top virus coat protein (CP) through the help of a leader sequence from N-terminal region of Cymbidium mosaic virus CP gene." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/74952327050024758393.

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碩士<br>朝陽科技大學<br>生化科技研究所碩士班<br>100<br>Banana bunchy top virus (BBTV) is taxonomically belonging to the genera of Babuvirus of Nanoviridae family. It infects banana systemically and induces symptoms of stunting, shortening of internodes, small and upward leaves and witches’ broom like of apical stem. Dark greenish lines can be observed on the diseased leaves, midribs, and petioles and the leaf margins turn frequently from yellowing into necrotic that weaken significantly the vigor of the infected plants. Besides aphid transmission, the virus can also be easily transmitted by vegetatively prop

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