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Journal articles on the topic 'Bananas Somatic embryogenesis'

Author: Grafiati

Published: 11 December 2022

Last updated: 31 July 2025

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1

Adero, Mark, Jaindra Nath Tripathi, and Leena Tripathi. "Advances in Somatic Embryogenesis of Banana." International Journal of Molecular Sciences 24, no. 13 (2023): 10999. http://dx.doi.org/10.3390/ijms241310999.

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The cultivation of bananas and plantains (Musa spp.) holds significant global economic importance, but faces numerous challenges, which may include diverse abiotic and biotic factors such as drought and various diseases caused by fungi, viruses, and bacteria. The genetic and asexual nature of cultivated banana cultivars makes them unattractive for improvement via traditional breeding. To overcome these constraints, modern biotechnological approaches like genetic modification and genome editing have become essential for banana improvement. However, these techniques rely on somatic embryogenesis

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2

Handayani, Tri, Apriliana Dyah Prawestri, Diyah Martanti, et al. "An Efficient Somatic Embryogenesis and Plant Regeneration from Immature Embryo of Wild Banana Musa acuminata ssp. malaccensis." Journal of Tropical Crop Science 12, no. 01 (2025): 172–84. https://doi.org/10.29244/jtcs.12.01.172-184.

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Wild banana Musa acuminata ssp. malaccensis, an ancestor of cultivated bananas, possesses valuable genetic diversity, including resistance genes to fusarium wilt, and demonstrates high environmental adaptability. These traits are important for pre-breeding programs, whether by conventional breeding, which is a lengthy process, or by taking advantage of somatic cell manipulation techniques such as somatic hybridization, which requires an efficient plant regeneration system like somatic embryogenesis. We have established an efficient and comprehensive protocol for somatic embryogenesis of this w

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3

Dhiya Nabilla Ardhani, Awang Maharijaya, and Megayani Sri Rahayu. "Callus formation response from immature male flower explant of plantain banana (Musa acuminata x Musa balbisiana cv. Kepok) treated by 2,4-D and BAP." Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) 52, no. 1 (2024): 101–9. http://dx.doi.org/10.24831/jai.v52i1.49008.

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Callus induction is an important step in indirect somatic embryogenesis. The aim of this study was to obtain an optimal medium for the callus formation of plantain bananas (cv. Kepok). The research was conducted from May to August 2022 at the Tissue Culture Laboratory of PT. ITCI Kartika Utama, Penajam Paser Utara District, East Kalimantan. The explant used an immature male flower of plantain banana (cv. Kepok). The experiment used a completely randomized design with two factors concentration of 2,4-D (1,2 and 4 ppm) and BAP (5, 10, and 15 ppm). The appearance of callus was monitored daily unt

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4

Natarajan, Nandhakumar, Sathish Sundararajan, Sathishkumar Ramalingam, and Perinba Suresh Chellakan. "Efficient and rapid in-vitro plantlet regeneration via somatic embryogenesis in ornamental bananas (Musa spp.)." Biologia 75, no. 2 (2019): 317–26. http://dx.doi.org/10.2478/s11756-019-00358-0.

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5

Zhao, Xiaobing, Yiting Zhuang, Wangyang Xie, et al. "Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group)." Plants 14, no. 5 (2025): 761. https://doi.org/10.3390/plants14050761.

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The regulatory mechanisms underlying embryogenic callus (EC) formation in polyploid bananas remain unexplored, posing challenges for genetic transformation and biotechnological applications. Here, we conducted transcriptome sequencing on cultured explants, non-embryogenic callus, EC, and browning callus in the ABB cultivar ‘MJ’ (Musa spp. cv. Bengal). Our analysis of differentially expressed genes (DEGs) revealed significant enrichment in plant hormones, MAPK, and zeatin biosynthesis pathways. Notably, most genes in the MJ variety exhibited balanced expression of the A and B alleles, but A-spe

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6

Novak, F. J., R. Afza, M. Van Duren, M. Perea-Dallos, B. V. Conger, and Tang Xiaolang. "Somatic Embryogenesis and Plant Regeneration in Suspension Cultures of Dessert (AA and AAA) and Cooking (ABB) Bananas (Musa spp.)." Nature Biotechnology 7, no. 2 (1989): 154–59. http://dx.doi.org/10.1038/nbt0289-154.

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7

Khaskheli, Allah Jurio, Muharam Ali, Shahla Baloch, et al. "Regulation of Callus Induction Efficiency in Banana Using Dichlorophenoxyacetic Acid in combination with Benzylaminopurine." Journal of Horticulture and Agricultural Sciences 1, no. 01 (2024): 28–38. https://doi.org/10.63459/jhas.2024.1.04.

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The regulation of callus induction in banana (Musa spp.) was investigated using plant growth regulators, specifically 2,4 dichlorophenoxyacetic acid (2,4 D) combined with other growth regulators to enhance callus formation efficiency. Different concentrations of 2,4 D, indole 3 acetic acid (IAA), and benzylaminopurine (BAP) were added to Murashige and Skoog (MS) basal medium to evaluate their effects on callus induction frequency, initiation time, proliferation efficiency and the number of regenerated calli. Results demonstrated that callus induction was significantly modulated by hormone conc

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8

Grapin, A., J. Schwendiman, and C. Teisson. "Somatic embryogenesis in plantain banana." In Vitro Cellular & Developmental Biology - Plant 32, no. 2 (1996): 66–71. http://dx.doi.org/10.1007/bf02823133.

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9

Handayani, Tri, Diyah Martanti, Apriliana Dyah Prawestri, et al. "Histological Analysis of Somatic Embryogenesis from Immature Zygotic Embryo of Wild Banana Musa acuminata ssp. malaccensis." Journal of Tropical Crop Science 11, no. 03 (2024): 307–19. http://dx.doi.org/10.29244/jtcs.11.03.307-319.

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Somatic embryogenesis, a crucial plant regeneration method, has become indispensable for crop improvement, particularly for species reliant on somatic cell manipulation techniques. Optimization of this process necessitates an understanding of the developmental stages involved. This study investigates the histological aspects of somatic embryogenesis in Musa acuminata ssp. malaccensis derived from immature zygotic embryos. Through detailed histological analysis, we aimed to elucidate the morphological changes and cellular organization occurring during the various stages of somatic embryogenesis

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10

P D, Smitha, Binoy K R, and Ashalatha S Nair. "Identification of Serk Gene from Bract Derived Embryogenic and Non - Embryogenic Calli of Four Diploid Banana Cultivars from South India." Journal of Agricultural Studies 5, no. 4 (2017): 161. http://dx.doi.org/10.5296/jas.v5i4.12213.

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Somatic embryogenesis receptor kinase (SERK) gene is known to be a marker of somatic embryogenesis in several plant species. The present study reported the presence of SERK gene from bract derived embryogenic calli bearing somatic embryos. The analysis of the expression pattern of the SERK gene during embryogenic cell formation and somatic embryogenesis revealed that SERK expression continued during pro embryogenic mass formation. In the present study the amplified product of cDNA from the somatic embryos has molecular size 1459 bp. The non- embryogenic callus also showed the presence of faint

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11

Chang, Shenghe, Zhengjing Wu, Wei Sun, et al. "A Method to Improve the Embryogenesis Rate of Banana Somatic Cell Embryogenesis." American Journal of Plant Sciences 09, no. 03 (2018): 531–41. http://dx.doi.org/10.4236/ajps.2018.93040.

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12

Lekshmi, R. S., K. B. Soni, Swapna Alex, Deepa S. Nair, Lekha Sreekantan, and B. R. Reghunath. "A Rapid Protocol for Somatic Embryogenesis Mediated Regeneration in Banana (Musa Spp.) Cv. Nendran." Journal of Horticultural Sciences 11, no. 2 (2016): 116–23. http://dx.doi.org/10.24154/jhs.v11i2.81.

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A simple and rapid protocol for somatic embryogenesis in banana cv. Nendran (AAB) using immature male flowers (IMF) has been developed. The IMF produced palewhite to yellow, globular embryogenic callus on MS medium supplemented with BA (0.05 - 0.50mgL-1) and picloram (0.50 - 2.00mgL-1) with explant response of to 30 per cent. Addition of ascorbic acid (20mgL-1) and Gelrite© (0.45 per cent) to callus induction medium reduced interference from phenolic exudation. Embryogenesis was induced (33.3 to 60 per cent) on semisolid (0.30 per cent Gelrite©) MS medium supplemented with BA 2mgL-1 + IAA 0.5m

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13

Morais-Lino, Lucymeire Souza, Janay Almeida Santos-Serejo, Edson Perito Amorim, José Raniere Ferreira de Santana, Moacir Pasqual, and Sebastião de Oliveira e Silva. "Somatic embryogenesis, cell suspension, and genetic stability of banana cultivars." In Vitro Cellular & Developmental Biology - Plant 52, no. 1 (2015): 99–106. http://dx.doi.org/10.1007/s11627-015-9729-2.

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14

Haïcour, Robert, Akym Assani, Viet BuiTrang, and Abdelkarim Guedira. "Protoplast isolation and culture for banana regeneration via somatic embryogenesis." Fruits 64, no. 4 (2009): 261–69. http://dx.doi.org/10.1051/fruits:2009021.

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15

Houllou-Kido, Laureen Michelle, Ederson Akio Kido, Maria Cristina Falco, et al. "Somatic embryogenesis and the effect of particle bombardment on banana Maçã regeneration." Pesquisa Agropecuária Brasileira 40, no. 11 (2005): 1081–86. http://dx.doi.org/10.1590/s0100-204x2005001100005.

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A plant regeneration method with cell suspension cultures of banana, and the effect of biobalistic on regeneration potential are described in this report. Somatic embryos of banana were obtained from indirect embryogenesis of male inflorescence of banana cultivar Maçã (AAB group). Part of the calluses formed (40%) showed embryogenic characteristics (nonfriable, compact and yellow color). The cell suspension, originated from embryogenic calluses, contained clusters of small tightly packed cells with dense cytoplasms, relatively large nuclei and very dense nucleoli. After four months of culture,

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16

Parra Peñalosa, Oscar José, and Giovanni Orlando Cancino Escalante. "Evaluation of induction of somatic embryogenesis from cotyledonary leaves of Banana Passion fruit (Passiflora mollissima) L.H Bailey." Respuestas 24, no. 3 (2019): 31–38. http://dx.doi.org/10.22463/0122820x.1847.

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Passiflora mollissima L.H Bailey is an endemic species from the Andes mountain of Colombia in South America with important edible fruits and medicinal and economical properties. The development of improved micropropagation techniques is necessary to provide rapid and efficient clonal propagation of elite genotypes with high resistance and uniform production, as well as a system that can be used for genetic transformation. For this reason, the investigation focused on the evaluation of induction of somatic embryogenesis in P. mollissima from cotyledonary leaves, the effect of growth regulator c

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17

Shu, H., L. Xu, Z. Li, J. Li, Z. Jin, and S. Chang. "Tobacco Arabinogalactan Protein NtEPc Can Promote Banana (Musa AAA) Somatic Embryogenesis." Applied Biochemistry and Biotechnology 174, no. 8 (2014): 2818–26. http://dx.doi.org/10.1007/s12010-014-1228-0.

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18

Maldonado-Borges, Josefina Ines, José Roberto Ku-Cauich, and Rosa Maria Escobedo-GraciaMedrano. "Annotation of Differentially Expressed Genes in the Somatic Embryogenesis ofMusaand Their Location in the Banana Genome." Scientific World Journal 2013 (2013): 1–7. http://dx.doi.org/10.1155/2013/535737.

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Analysis of cDNA-AFLP was used to study the genes expressed in zygotic and somatic embryogenesis ofMusa acuminataColla ssp.malaccensis, and a comparison was made between their differential transcribed fragments (TDFs) and the sequenced genome of the double haploid- (DH-) Pahang of themalaccensissubspecies that is available in the network. A total of 253 transcript-derived fragments (TDFs) were detected with apparent size of 100–4000 bp using 5 pairs of AFLP primers, of which 21 were differentially expressed during the different stages of banana embryogenesis; 15 of the sequences have matched D

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19

Panis, Bart, Ann Van Wauwe, and Rony Swennen. "Plant regeneration through direct somatic embryogenesis from protoplasts of banana (Musa spp.)." Plant Cell Reports 12-12, no. 7-8 (1993): 403–7. http://dx.doi.org/10.1007/bf00234701.

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20

Kosky, Rafael GÃ³mez. "Secondary multiplication of somatic embryos in banana (Musa spp. AAA) in semisolid medium: effect of the culture vessel type and sterilization method." JOURNAL OF ADVANCES IN BIOTECHNOLOGY 4, no. 2 (2014): 352–57. http://dx.doi.org/10.24297/jbt.v4i2.5001.

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The objective of this work was to evaluate the influence of the type of culture vessel, and sterilization method on secondary multiplication of somatic embryos of the banana cultivar Grande naine (AAA) in semisolid culture medium. From 200 Âµl of embryogenic cell aggregates, somatic embryos in the globular stage were formed, which were used in the experiments to compare the types of culture vessel (glass and plastic) and the form of sterilization â€“ humid heat (autoclave) and chemical (VitrofuralÂ®). The evaluations of the number of somatic embryos that were multiplied were done at 15 and 20

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21

Cronauer-Mitra, Sandra S., and A. D. Krikorian. "Plant regeneration via somatic embryogenesis in the seeded diploid banana Musa ornata Roxb." Plant Cell Reports 7, no. 1 (1988): 23–25. http://dx.doi.org/10.1007/bf00272970.

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22

Khalil, S., K. Cheah, E. Perez, D. Gaskill, and J. Hu. "Regeneration of banana (Musa spp. AAB cv. Dwarf Brazilian) via secondary somatic embryogenesis." Plant Cell Reports 20, no. 12 (2002): 1128–34. http://dx.doi.org/10.1007/s00299-002-0461-0.

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23

Morais-Lino, Lucymeire Souza, Janay Almeida dos Santos-Serejo, Sebastião de Oliveira e. Silva, José Raniere Ferreira de Santana, and Adilson Kenji Kobayashi. "Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra." Pesquisa Agropecuária Brasileira 43, no. 10 (2008): 1325–30. http://dx.doi.org/10.1590/s0100-204x2008001000010.

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The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell

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24

Escalant, Jean-Vincent, Claude Teisson, and François Cote. "Amplified somatic embryogenesis from male flowers of triploid banana and plantain cultivars (Musa spp.)." In Vitro Cellular & Developmental Biology - Plant 30, no. 4 (1994): 181–86. http://dx.doi.org/10.1007/bf02823029.

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25

Rustagi, Anjana, Shashi Shekhar, Deepak Kumar, Kapil Lawrence, Vishnu Bhat, and Neera Bhalla Sarin. "High speed regeneration via somatic embryogenesis in elite Indian banana cv. Somrani monthan (ABB)." Vegetos 32, no. 1 (2019): 39–47. http://dx.doi.org/10.1007/s42535-019-00005-8.

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26

P. D, Smitha, Binoy kr, and Ashalatha S. Nair. "Enhanced Secondary Somatic Embryogenesis in Suspension Culture of Four Diploid Banana Cultivars from Kerala." International Journal of Fruit Science 20, sup2 (2020): S617—S626. http://dx.doi.org/10.1080/15538362.2020.1753138.

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27

P D, Smitha, Binoy KR, and Ashalatha S. Nair. "Enhanced Secondary Somatic Embryogenesis in Suspension Culture of Four Diploid Banana Cultivars from Kerala." International Journal of Fruit Science 20, sup2 (2020): S695—S704. http://dx.doi.org/10.1080/15538362.2020.1768615.

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28

Ramírez-Villalobos, M., and E. de García. "SECONDARY SOMATIC EMBRYOGENESIS IN BANANA CIEN-BTA-03 (MUSA SP. AAAA) AND REGENERATION OF PLANTS." Acta Horticulturae, no. 829 (June 2009): 45–50. http://dx.doi.org/10.17660/actahortic.2009.829.4.

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29

Shchukin, A., D. Ben-Bassat, and Y. Israeli. "PLANT REGENERATION VIA SOMATIC EMBRYOGENESIS IN GRAND NAIN BANANA AND ITS EFFECT ON SOMACLONAL VARIATION." Acta Horticulturae, no. 447 (October 1997): 317–18. http://dx.doi.org/10.17660/actahortic.1997.447.62.

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30

Xu, Chunxiang, Lu Zhao, Xiao Pan, and Jozef Šamaj. "Developmental Localization and Methylesterification of Pectin Epitopes during Somatic Embryogenesis of Banana (Musa spp. AAA)." PLoS ONE 6, no. 8 (2011): e22992. http://dx.doi.org/10.1371/journal.pone.0022992.

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31

Ponni, T. G., and Ashalatha S. Nair. "Somatic embryogenesis to overcome low seed viability and conserve wild banana (Ensete superbum (Roxb.) Cheesman)." In Vitro Cellular & Developmental Biology - Plant 55, no. 4 (2019): 371–79. http://dx.doi.org/10.1007/s11627-019-09998-y.

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32

Singh, A. K., N. K. Mishra, Sanjeev Kumar, and Sudhakar Pandey. "Application of somatic hybridization for the improvement of horticultural crops." Horticultural Biotechnology Research 1 (December 5, 2015): 39. http://dx.doi.org/10.19071/jhcbt.2015.v1.2897.

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<p>Somatic hybridization (SH) using protoplast fusion is a promising tool to produce symmetrical and asymmetrical polyploidy somatic hybrids in many agricultural crops. The technique of SH could facilitate conventional breeding by providing of novel lines so as to use them as elite breeding materials in conventional crosses for both scion and rootstock improvement. Further, SH can overcome those problems associated with sexual hybridization viz., sexual incompatibility, nucellar embryogenesis, and male/female sterility. Successful exploitation of SH in horticultural crops mainly comes fr

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33

Shivani, Praveen Awasthi, Vikrant Sharma, et al. "Genome-wide analysis of transcription factors during somatic embryogenesis in banana (Musa spp.) cv. Grand Naine." PLOS ONE 12, no. 8 (2017): e0182242. http://dx.doi.org/10.1371/journal.pone.0182242.

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34

Kulkarni, V. M., and V. A. Bapat. "Somatic embryogenesis and plant regeneration from cell suspension cultures of Rajeli (AAB), an endangered banana cultivar." Journal of Plant Biochemistry and Biotechnology 22, no. 1 (2012): 132–37. http://dx.doi.org/10.1007/s13562-012-0119-0.

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35

Remakanthan, A., Tara G. Menon, and E. V. Soniya. "Somatic embryogenesis in banana (Musa acuminata AAA cv. Grand Naine): effect of explant and culture conditions." In Vitro Cellular & Developmental Biology - Plant 50, no. 1 (2013): 127–36. http://dx.doi.org/10.1007/s11627-013-9546-4.

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36

Xu, Chunxiang, Tomáš Takáč, Christian Burbach, Diedrik Menzel, and Jozef Šamaj. "Developmental localization and the role of hydroxyproline rich glycoproteins during somatic embryogenesis of banana (Musa spp. AAA)." BMC Plant Biology 11, no. 1 (2011): 38. http://dx.doi.org/10.1186/1471-2229-11-38.

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37

Mohandas, Sukhada, H. D. Sowmya, and S. Meenakshi. "Somatic embryogenesis and plant regeneration through cell suspension in diploid (AB) banana cultivar Elakki Bale (syn Neypoovan)." Journal of Plant Biochemistry and Biotechnology 22, no. 2 (2012): 245–49. http://dx.doi.org/10.1007/s13562-012-0125-2.

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38

Moradi, Zahra, Farah Farahani, Masoud Sheidai, and Taher Nejad Satari. "Somaclonal variation in banana (Musa acuminate collacv. Valery) regenerated plantlets from somatic embryogenesis: histological and cytogenetic approaches." Caryologia 70, no. 1 (2016): 1–6. http://dx.doi.org/10.1080/00087114.2016.1198665.

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39

Jekayinoluwa, Temitope, Jaindra Nath Tripathi, George Obiero, Edward Muge, and Leena Tripathi. "Phytochemical Analysis and Establishment of Embryogenic Cell Suspension and Agrobacterium-mediated Transformation for Farmer Preferred Cultivars of West African Plantain (Musa spp.)." Plants 9, no. 6 (2020): 789. http://dx.doi.org/10.3390/plants9060789.

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Banana and plantain are among the foremost staple food crops providing food and livelihood to over 500 million people in tropical countries. Despite the importance, their production is hampered due to several biotic and abiotic stresses. Plant tissue culture techniques such as somatic embryogenesis and genetic transformation offer a valuable tool for genetic improvement. Identification and quantification of phytochemicals found in banana and plantain are essential in optimizing in vitro activities for crop improvement. Total antioxidants, phenolics, flavonoids, and tannins were quantified in v

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40

Tran, T. H., T. V. Bui, and T. Y. Feng. "The role of auxin and cytokinin on somatic embryogenesis from cell suspension cultures of the banana cultivar ‘Cau Man’." Acta Horticulturae, no. 1114 (March 2016): 219–26. http://dx.doi.org/10.17660/actahortic.2016.1114.30.

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41

Assani, A., R. Haicour, G. Wenzel, et al. "Plant regeneration from protoplasts of dessert banana cv. Grande Naine (Musa spp., Cavendish sub-group AAA) via somatic embryogenesis." Plant Cell Reports 20, no. 6 (2001): 482–88. http://dx.doi.org/10.1007/s002990100366.

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42

Pan, Xiao, Xiao Yang, Guimei Lin, et al. "Ultrastructural changes and the distribution of arabinogalactan proteins during somatic embryogenesis of banana (Musa spp. AAA cv. ‘Yueyoukang 1’)." Physiologia Plantarum 142, no. 4 (2011): 372–89. http://dx.doi.org/10.1111/j.1399-3054.2011.01478.x.

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43

Aspuria, Evalour, Emmanuel Bernardo, and Maria Angie Tayangona. "Development of a Cell Suspension Protocol for Abaca (Musa textilis Nee ‘Inosa’)." Philippine Agricultural Scientist 103, no. 3 (2020): 179–90. https://doi.org/10.62550/ev10053019.

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A protocol for the establishment of embryogenic cell suspension in abaca was developed based on the International Network for the Improvement of Banana and Plantain (INIBAP) procedure for Musa. Meristematic buds from shoot cultures were excised and cultured on P5 medium for several cycles until ‘scalps’ formed. These scalps were then used to produce embryogenic complexes (ECs) in 2,4-D-containing media. Discrete primary somatic embryos (SEs) were observed on ECs 3–4 wk after initial culture. After 6 mo, 6 % of the 150 inoculated scalps formed into ECs that had at least 10 SEs. These were then

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44

FILIPPI, SILVIA BALBÃO, BEATRIZ APPEZZATO-DA-GLÓRIA, and ADRIANA PINHEIRO MARTINELLI RODRIGUEZ. "Histological changes in banana explants, cv. Nanicão (Musa spp., Group AAA), submitted to different auxins for induction of somatic embryogenesis." Revista Brasileira de Botânica 24, no. 4 (2001): 595–602. http://dx.doi.org/10.1590/s0100-84042001000500015.

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45

Chung, Jen-Ping, Tsu-Liang Chang, Albert Yu-Ming Chi, and Chou Tou Shii. "Triploid banana cell growth phases and the correlation of medium pH changes with somatic embryogenesis in embryogenic cell suspension culture." Plant Cell, Tissue and Organ Culture 87, no. 3 (2006): 305–14. http://dx.doi.org/10.1007/s11240-006-9168-1.

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46

Pérez-Hernández, Juan Bernardo, and Purificación Rosell-García. "Inflorescence proliferation for somatic embryogenesis induction and suspension-derived plant regeneration from banana (Musa AAA, cv. ‘Dwarf Cavendish’) male flowers." Plant Cell Reports 27, no. 6 (2008): 965–71. http://dx.doi.org/10.1007/s00299-008-0509-x.

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47

Wang, Weiming, La Shaun Sanders, Alice Perry, and Ebonee Duncan. "High throughput in vitro production of somatic embryoswith innovative cryo cell banking system." Horticulture International Journal 6, no. 3 (2022): 157–60. http://dx.doi.org/10.15406/hij.2022.06.00259.

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ArborGen Inc. was originally created as a partnership for forestry research in 2000 by West Rock (MWV), International Paper and Rubicon (Fletcher Challenge). It now produces the largest number of both conventional and advanced genetic tree seedlings in the world. Among its advanced genetics offerings, varietals have the highest level of genetic traits in yield and timber quality such as height, diameter growth, disease resistance and etc. To scale up varietals, in vitro process named somatic embryogenesis (SE) is used as a mean to mass propagate SE seedlings. Over the years of R&D efforts,

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48

KHALIL, S. "REGENERATION OF BANANA (Musa spp. AAA Group) cv. WILLIAMS via SECONDARY SOMATIC EMBRYOGENESIS AND CELL SUSPENSION FROM IMMATURE MALE FLOWER BUDS." Egyptian Journal of Genetics and Cytology 41, no. 2 (2012): 337–52. http://dx.doi.org/10.21608/ejgc.2012.10548.

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49

Dai, Xue-Mei, Wang Xiao, Xia Huang, Jie-Tang Zhao, Yun-Feng Chen, and Xue-Lin Huang. "Plant regeneration from embryogenic cell suspensions and protoplasts of dessert banana cv. ‘Da Jiao’ (Musa paradisiacal ABB Linn.) via somatic embryogenesis." In Vitro Cellular & Developmental Biology - Plant 46, no. 5 (2010): 403–10. http://dx.doi.org/10.1007/s11627-010-9314-7.

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Gómez Kosky, Rafael, Luis Antonio Barranco, Borys Chong Pérez, Dion Daniels, Maritza Reyes Vega, and Manuel de Feria Silva. "Trueness-To-Type and Yield Components of the Banana Hybrid Cultivar FHIA-18 Plants Regenerated Via Somatic Embryogenesis in a Bioreactor." Euphytica 150, no. 1-2 (2006): 63–68. http://dx.doi.org/10.1007/s10681-006-9093-8.

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