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Journal articles on the topic 'Band Cell Count'

Author: Grafiati

Published: 7 June 2025

Last updated: 2 August 2025

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1

Sudipta, Bhattacharyya, Sarkar Krishanu, Kumar Mandal Abhishek, and Bhattacharyya Samriddha. "The Sensitivity and Specificity of Different Laboratory Parameters in Septicemia in Paediatric Age Group Patients: An Observational Study Conducted in a Rural Medical College Hospital." International Journal of Pharmaceutical and Clinical Research 16, no. 12 (2024): 1482–87. https://doi.org/10.5281/zenodo.14613627.

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<strong>Introduction:</strong> Sepsis, one of the leading causes of severe illness worldwide, is a potentially fatal organ failure brought on by a dysregulated host response to infection. The aim of present study is to evaluate the specificity and sensitivity of C-reactive protein (CRP), procalcitonin (PCT), Band cell count and toxic granules in septicemia in pediatric age group. <strong>Material and Methods: </strong>The present observational study, was conducted in a government rural medical college hospital catering mostly to the rural population under its jurisdiction for a

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Lapić, Ivana, Brankica Šimac, Martina Horvat, et al. "The band count imprecision – a Croatian multicentric pilot study." Biochemia medica 34, no. 2 (2024): 335–39. http://dx.doi.org/10.11613/bm.2024.020803.

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Graphical abstract Highlights • Band counting is unreliable practice due to the high inter-observer variability • 2015 International Council for Standardization in Haematology guidelines recommend to count band neutrophils as segmented neutrophils • The inclusion of bands within the white blood cell differential is still used in Croatia • There is a very high variability in enumerating bands among Croatian laboratories • There is a need for national recommendations that will endorse ICSH guidelines IntroductionDue to high inter-observer variability the 2015 International Council for Standardiz

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Kizhina, Aleksandra, Lyudmila Uzenbaeva, Ekaterina Antonova, Vladimir Belkin, Viktor Ilyukha, and Evgeniy Khizhkin. "Hematological parameters in hibernating Eptesicus nilssonii (Mammalia: Chiroptera) collected in Northern European Russia." Acta Chiropterologica 20, no. 1 (2018): 273–83. https://doi.org/10.5281/zenodo.13454662.

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(Uploaded by Plazi for the Bat Literature Project) The Northern bat, Eptesicus nilssonii, is the most common species among northern chiropteran populations. The species is noted for its long hibernation period with low mortality rate. We investigated the morphology of E. nilssonii blood cells during hibernation, hematological parameters including hemoglobin level, red blood cell (RBC) count, total white blood cell (WBC) count, and differential WBC count. The study revealed changes of several hematological parameters in hibernating bats, with some variation during the hibernation season. Hibern

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Kizhina, Aleksandra, Lyudmila Uzenbaeva, Ekaterina Antonova, Vladimir Belkin, Viktor Ilyukha, and Evgeniy Khizhkin. "Hematological parameters in hibernating Eptesicus nilssonii (Mammalia: Chiroptera) collected in Northern European Russia." Acta Chiropterologica 20, no. 1 (2018): 273–83. https://doi.org/10.5281/zenodo.13454662.

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(Uploaded by Plazi for the Bat Literature Project) The Northern bat, Eptesicus nilssonii, is the most common species among northern chiropteran populations. The species is noted for its long hibernation period with low mortality rate. We investigated the morphology of E. nilssonii blood cells during hibernation, hematological parameters including hemoglobin level, red blood cell (RBC) count, total white blood cell (WBC) count, and differential WBC count. The study revealed changes of several hematological parameters in hibernating bats, with some variation during the hibernation season. Hibern

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Kizhina, Aleksandra, Lyudmila Uzenbaeva, Ekaterina Antonova, Vladimir Belkin, Viktor Ilyukha, and Evgeniy Khizhkin. "Hematological parameters in hibernating Eptesicus nilssonii (Mammalia: Chiroptera) collected in Northern European Russia." Acta Chiropterologica 20, no. 1 (2018): 273–83. https://doi.org/10.5281/zenodo.13454662.

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(Uploaded by Plazi for the Bat Literature Project) The Northern bat, Eptesicus nilssonii, is the most common species among northern chiropteran populations. The species is noted for its long hibernation period with low mortality rate. We investigated the morphology of E. nilssonii blood cells during hibernation, hematological parameters including hemoglobin level, red blood cell (RBC) count, total white blood cell (WBC) count, and differential WBC count. The study revealed changes of several hematological parameters in hibernating bats, with some variation during the hibernation season. Hibern

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6

Kizhina, Aleksandra, Lyudmila Uzenbaeva, Ekaterina Antonova, Vladimir Belkin, Viktor Ilyukha, and Evgeniy Khizhkin. "Hematological parameters in hibernating Eptesicus nilssonii (Mammalia: Chiroptera) collected in Northern European Russia." Acta Chiropterologica 20, no. 1 (2018): 273–83. https://doi.org/10.5281/zenodo.13454662.

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(Uploaded by Plazi for the Bat Literature Project) The Northern bat, Eptesicus nilssonii, is the most common species among northern chiropteran populations. The species is noted for its long hibernation period with low mortality rate. We investigated the morphology of E. nilssonii blood cells during hibernation, hematological parameters including hemoglobin level, red blood cell (RBC) count, total white blood cell (WBC) count, and differential WBC count. The study revealed changes of several hematological parameters in hibernating bats, with some variation during the hibernation season. Hibern

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7

Kizhina, Aleksandra, Lyudmila Uzenbaeva, Ekaterina Antonova, Vladimir Belkin, Viktor Ilyukha, and Evgeniy Khizhkin. "Hematological parameters in hibernating Eptesicus nilssonii (Mammalia: Chiroptera) collected in Northern European Russia." Acta Chiropterologica 20, no. 1 (2018): 273–83. https://doi.org/10.5281/zenodo.13454662.

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(Uploaded by Plazi for the Bat Literature Project) The Northern bat, Eptesicus nilssonii, is the most common species among northern chiropteran populations. The species is noted for its long hibernation period with low mortality rate. We investigated the morphology of E. nilssonii blood cells during hibernation, hematological parameters including hemoglobin level, red blood cell (RBC) count, total white blood cell (WBC) count, and differential WBC count. The study revealed changes of several hematological parameters in hibernating bats, with some variation during the hibernation season. Hibern

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8

Yilmaz, Hayri Levent, Riza Dincer Yildizdas, Nazan Alparslan, Kenan Ozcan, Akgun Yaman, and Filiz Kibar. "Screening Tools for Bacteraemia in a Selected Population of Febrile Children." Annals of the Academy of Medicine, Singapore 37, no. 3 (2008): 192–99. http://dx.doi.org/10.47102//annals-acadmedsg.v37n3p192.

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Introduction: This is a prospective, observational study. The aims of the study were to determine the rate of bacteraemia in febrile children in Turkey, and to evaluate the usefulness of white blood cell (WBC) count and manual differential counts of peripheral blood smears and a RISK score in predicting bacteraemia among these children. Materials and Methods: A total of 377 febrile children aged 3 to 36 months were included in the study. Complete blood cell (CBC) count, manual differential counts and blood cultures were performed in all patients. The main outcome measures used to evaluate the

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9

Adams, R., F. B. Garry, B. M. Aldridge, M. D. Holland, and K. G. Odde. "Hematologic values in newborn beef calves." American Journal of Veterinary Research 53, no. 6 (1992): 944–50. http://dx.doi.org/10.2460/ajvr.1992.53.06.944.

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Summary Hematologic values were determined in 35 beef calves at birth, at 24 and 48 hours, and in 22 of these calves at 3 weeks after birth. Thirty calves did not have clinical signs of disease throughout the 3-week period. Variables that changed significantly over time in these healthy calves included hematocrit, rbc count, hemoglobin concentration, mean cell volume, mean cell hemoglobin concentration, wbc count, neutrophil-to-lymphocyte ratio, and plasma total protein and serum immunoglobulin concentrations. Of the 35 calves, 5 had clinical signs of disease at 3 weeks. Comparison of hematolo

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Jelek, Jolica, Jole Stark, Marija Nemec, et al. "The influence of age, farm, and physiological status on pig hematological profiles." Journal of Swine Health and Production 26, no. 2 (2018): 72–78. http://dx.doi.org/10.54846/jshap/1049.

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Objectives: To evaluate influence of age, farm, and physiological status on pig hematological profiles. Materials and methods: This study was carried out on five 1-site, farrow-to-finish pig farms in Slovenia, where a total of 382 clinically normal pigs were sampled. All farms were free of Aujeszky’s disease (pseudorabies), classical swine fever, and porcine reproductive and respiratory syndrome. Blood samples were taken from the anterior vena cava. Hematological analyses were performed with an automated hematological analyser. The following hematological variables were measured: red blood cel

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11

Colletto, G. M. D. Dal, D. W. Fulker, O. C. de O. Barretto, and M. Kolya. "Genetic and Environmental Effects on Blood Cells." Acta geneticae medicae et gemellologiae: twin research 42, no. 3-4 (1993): 245–52. http://dx.doi.org/10.1017/s000156600000324x.

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AbstractIn a sample of 105 concordant sex MZ and DZ twin pairs, the following characteristics were measured: red cell count, haemoglobin concentration, package cell volume, mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, reticulocytes, platelets, white cell count and the six types of leucocytes, lymphocytes, monocytes, band and segmented neutrophils, eosinophils and basophils. The statistical model employed in the univariate twin analysis allows for three sources of variation: genetic (h2), shared environmental (c2) and specific environmental influences (e2). A ge

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12

Goncharov, S. "The morphological changes in the blood of the predatory fish species with eustrongylidosis." Scientific Messenger of LNU of Veterinary Medicine and Biotechnology 21, no. 93 (2019): 15–20. http://dx.doi.org/10.32718/nvlvet9303.

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The article presents the results of the research concerning the morphological changes in the blood of the predatory fish species (perch, pike and zander) сaused by nematodical parasitic disease – eustrongylidosis. The research has been conducted in 2014–2018. It has been found that in the blood of perch with eustrongylidosis the hemoglobin and the red-blood-cell count decreased by 6.87 and 42.11 respectively, the white-blood-cell count increased by 8.95%; the basophils and pseudobasophils count – by 129.17%; the eosinophils and pseudoeosinophils count – by 131%; the number of band and segmente

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Leport, Catherine, Jacqueline Franck, Genevieve Chene, et al. "Immunoblot Profile as Predictor of Toxoplasmic Encephalitis in Patients Infected with Human Immunodeficiency Virus." Clinical Diagnostic Laboratory Immunology 8, no. 3 (2001): 579–84. http://dx.doi.org/10.1128/cdli.8.3.579-584.2001.

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ABSTRACT In order to define more accurately human immunodeficiency virus-infected patients at risk of developing toxoplasmic encephalitis (TE), we assessed the prognostic significance of the anti-Toxoplasma gondii immunoglobulin G (IgG) immunoblot profile, in addition to AIDS stage, a CD4+ cell count <50/mm3, and an antibody titer ≥150 IU/ml, in patients with CD4 cell counts <200/mm3 and seropositive forT. gondii. Baseline serum samples from 152 patients included in the placebo arm of the ANRS 005-ACTG 154 trial (pyrimethamine versus placebo) were used. The IgG immunoblot profile was det

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Carter, Caitlyn M., Laura C. Cregar, and Adam D. Aulbach. "Cytological Bone Marrow Cell Differential Counts and Morphologic Findings in Healthy Cynomolgus Monkeys (Macaca fascicularis) from Nonclinical Toxicology Studies." Toxicologic Pathology 45, no. 2 (2016): 267–74. http://dx.doi.org/10.1177/0192623316677067.

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Cytological bone marrow evaluation is utilized in nonclinical toxicology studies to characterize hematopoietic effects when the combined interpretation of histologic and complete blood count data does not yield sufficient information. Results from cytological bone marrow examination should be interpreted in the context of variability observed in concurrent control animals with consideration of cytologist experience and historical/published data. Cytological bone marrow differential counts and cellular morphologic findings from 130 (66 male, 64 female) healthy control cynomolgus monkeys from no

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15

Baker, Raymond C., John H. Seguin, Nancy Leslie, Mary J. R. Gilchrist, and Martin G. Myers. "Fever and Petechiae in Children." Pediatrics 84, no. 6 (1989): 1051–55. http://dx.doi.org/10.1542/peds.84.6.1051.

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A prospective study of patients with fever and petechiae was performed. Of 190 patients enrolled in the 1-year study, 13 (7%) had meningococcal disease. The most common bacterial association was Streptococcus pyogenes (19 patients). Viral infections were documented in 28 patients. Patients with invasive bacterial disease (group I) appeared more sick, were more likely to have signs of meningeal irritation, and were more likely to have petechiae on the lower extremities than those with less serious, nonbacteremic disease (group II). No patient in group I had petechiae only above the nipple line.

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Ahmed, Khawaja Abrar, Manzoor Ali Khan, Syed Zakir Naqvi, Ishtiaq Ahmed Mughal, Mohsna Saeed Zia, and Arzoo Idrees. "Combining Complete Blood Count with Differential Count; C-Reactive Protein and Band Neutrophils in Diagnosis of Early onset Neonatal Sepsis." Pakistan Journal of Medical and Health Sciences 16, no. 9 (2022): 168–69. http://dx.doi.org/10.53350/pjmhs22169168.

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Aim: To assess the diagnostic accuracy of complete blood count with band versus total neutrophil ratio and C-reactive proteins as an efficient analytical procedure for determining early onset sepsis in neonates. Study design: Cross sectional analytical study Place and duration of study: Department of Paediatric Medicine, Abbas Institute of Medical Sciences Muzafarabad, AJ Kashmir from 01-02-2022 to 31-07-2022. Methodology: Fifty patients were enrolled. Coulter counter was used for analyzing leucocytes of the neonates. Thin film-smears were generated by the laboratory scientist for identifying

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Sudnitsyna, Julia, Elisaveta Skverchinskaya, Irina Dobrylko, Elena Nikitina, Stepan Gambaryan, and Igor Mindukshev. "Microvesicle Formation Induced by Oxidative Stress in Human Erythrocytes." Antioxidants 9, no. 10 (2020): 929. http://dx.doi.org/10.3390/antiox9100929.

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Extracellular vesicles (EVs) released by different cell types play an important role in many physiological and pathophysiological processes. In physiological conditions, red blood cell (RBC)-derived EVs compose 4–8% of all circulating EVs, and oxidative stress (OS) as a consequence of different pathophysiological conditions significantly increases the amount of circulated RBC-derived EVs. However, the mechanisms of EV formation are not yet fully defined. To analyze OS-induced EV formation and RBC transformations, we used flow cytometry to evaluate cell esterase activity, caspase-3 activity, an

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Rabbani, Sahar, Muhammad Farooq, Samina Naeem, Nasir Uddin, Muhammad Abdul Naeem, and Shahneela Jabeen. "DIAGNOSTIC EFFICACY OF RED BLOOD CELL INDICES IN DIAGNOSIS OF BETA THALASSEMIA TRAIT TAKING HAEMOGLOBIN ELECTROPHORESIS AS GOLD STANDARD." PAFMJ 71, no. 3 (2021): 1006–10. http://dx.doi.org/10.51253/pafmj.v71i3.3905.

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Objective: To determine the diagnostic efficacy of haematological indices for the diagnosis of beta thalassemia trait taking haemoglobin electrophoresis as a gold standard. Study Design: Cross sectional study. Place and Duration of Study: Department of Haematology Combined Military Hospital Lahore, from Aug to Dec 2019. Methodology: Two hundred and six anaemic patients 20-70 years of age of both genders were included in the study. Blood was collected from all the patients and complete blood count were generated through automated haematology analyser Sysmex KX-21. Based on the

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Donlan, R. M., J. A. Piede, C. D. Heyes, et al. "Model System for Growing and Quantifying Streptococcus pneumoniae Biofilms In Situ and in Real Time." Applied and Environmental Microbiology 70, no. 8 (2004): 4980–88. http://dx.doi.org/10.1128/aem.70.8.4980-4988.2004.

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ABSTRACT Streptococcus pneumoniae forms biofilms, but little is known about its extracellular polymeric substances (EPS) or the kinetics of biofilm formation. A system was developed to enable the simultaneous measurement of cells and the EPS of biofilm-associated S. pneumoniae in situ over time. A biofilm reactor containing germanium coupons was interfaced to an attenuated total reflectance (ATR) germanium cell of a Fourier transform infrared (FTIR) laser spectrometer. Biofilm-associated cells were recovered from the coupons and quantified by total and viable cell count methods. ATR-FTIR spect

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Loken, Michael R., Sung-Chao Chu, Wayne K. Fritschle, Dian-Kun Li, and Denise A. Wells. "Correcting Flow Cytometric Blast Counts for Blood Contamination in Bone Marrow Aspirates." Blood 110, no. 11 (2007): 4613. http://dx.doi.org/10.1182/blood.v110.11.4613.4613.

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Abstract An accurate blast count is pivotal in the diagnosis, classification and prognosis of patients with myelodysplasia. Blast counts in all previous classification schemes are based on morphologic assessment of marrow aspirates with a poor correlation to blast counts determined by flow cytometry. A significant problem in blast enumeration by flow cytometry is the variable hemodilution of the marrow during collection for flow cytometric analysis. Blast counts can vary depending on which aspirate tube is used for flow analysis, e.g., 2.4%, 1st 5ml tube; 0.62%, 2nd 5ml tube; 0.58% 3rd 5ml tub

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Andonova, Maria, Dimitritchka Dimitrova, and I. Borissov. "Nonspecific defense mechanisms in dogs during experimental staphylococcal infection." Veterinarski glasnik 65, no. 1-2 (2011): 25–41. http://dx.doi.org/10.2298/vetgl1102025a.

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The aim of our study was to evaluate nonspecific defense mechanisms (phagocytosis and acute phase response) in dogs during an experimental infection caused by subcutaneous application of Staphylococcus aureus (1x108 CFU/mL). The neutrophils phagocytosis (evaluated by fluorescein isothiocyanate labeled Staphylococcus aureus) is activated at 48 h and the phagocytic number remained high at the 72-nd h of infection. During the early stage of the infection process (between the 2-nd and 24-th h) the absolute segmented neutrophil count, the absolute band count, band-neutrophyl ratio, tend to have hig

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Chaves, Fernando, Bethany Tierno, and Dongsheng Xu. "Neutrophil Volume Distribution Width: A New Automated Hematologic Parameter for Acute Infection." Archives of Pathology & Laboratory Medicine 130, no. 3 (2006): 378–80. http://dx.doi.org/10.5858/2006-130-378-nvdwan.

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Abstract Context.—The number of band forms and immature neutrophils increases during acute bacterial infection. However, the determination of band counts and other neutrophil morphologic changes, such as the presence of toxic granulation, toxic vacuolization, and Dohle bodies in the cytoplasm, is labor intensive and time consuming, as it requires manual examination by an experienced medical technologist. Objective.—To investigate the value of the neutrophil volume distribution width (NDW), generated by VCS technology of the Coulter LH 750 hematology analyzer, as an additional predictor of acut

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SONWANE, S. M., M. M. CHOPADE, M. P. SAWANE, et al. "Study of polymorphism of toll like receptor-4 gene in crossbred cattleand its association with somatic cell count." Indian Journal of Animal Sciences 93, no. 12 (2023): 1223–25. http://dx.doi.org/10.56093/ijans.v93i12.129102.

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The present study was undertaken to study the TLR4 gene polymorphism in exon 3 and its association with somatic cell count in HF×Gir cows using PCR-RFLP. Sixty crossbred cows (40 affected and 20 normal) between 3rd to 5th lactation were selected from Bombay Gowrakshak Mandali, Betegaon, Maharashtra. The confirmation of mastitis was done with the help of California MastitisTest (CMT) and the blood samples of 40 affected animals were collected aseptically along with 20 normal animals. The mean of somatic cell score in normal cows ranged between 2.86 to 2.99 × 105 cells/ml, whereas for affected a

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Catley, Laurence, Teru Hideshima, Dharminder Chauhan, et al. "Alkylphosphocholine Perifosine Inhibits Myeloma Cell Growth While Inducing Myeloid Hyperplasia in a Murine Myeloma Model." Blood 106, no. 11 (2005): 1579. http://dx.doi.org/10.1182/blood.v106.11.1579.1579.

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Abstract Perifosine is an alkylphosphocholine with structural similarity to phospholipids that are the main constituents of cellular membranes. It is the first orally available compound inducing AKT-inhibition in clinical trials. In myeloma tumor cells, Perifosine can inhibit Akt and induce apoptosis. Perifosine inhibits the growth of myeloma tumor cells in vivo and prolongs survival in a murine myeloma model. Triple immune deficient female beige-nude-xid (BNX) mice were inoculated with subcutaneous myeloma tumors derived from MM.1S cells. When measurable growing tumors appeared, mice were ran

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Kidd, John, Donald L. Batisky, Constantine A. Stratakis, Adolpho Garnica, Benjamin R. Waller, and Landon B. Pendergrass. "INDEX OF SUSPICION." Pediatrics In Review 16, no. 11 (1995): 433–36. http://dx.doi.org/10.1542/pir.16.11.433.

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This section of Pediatrics in Review reminds clinicians of those conditions that can present in a misleading fashion and require suspicion for early diagnosis. Emphasis has been placed on conditions in which early diagnosis is important and that the general pediatrician might be expected to encounter, at least once in a while. The reader is encouraged to write possible diagnoses for each case before turning to the discussion, which is on the following page. We invite readers to contribute case presentations and discussions. Case 1 Presentation A 4-year-old boy who has SC (sickle cell-hemoglobi

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Souza, Damaris Ferreira de, Edson Ferraz Evaristo de Paula, Sergio Rodrigo Fernandes, et al. "Dinâmica dos parâmetros hematológicos em cordeiras durante os primeiros quatro meses de vida." Semina: Ciências Agrárias 39, no. 6 (2018): 2465. http://dx.doi.org/10.5433/1679-0359.2018v39n6p2465.

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Important physiological changes affect the blood profile of ruminants during the growth phase, but few studies approach the factors involved in these dynamics in lambs. The aim of this study was to characterize the dynamics of hematological parameters, of total plasma protein (TPP), and of fibrinogen in healthy female lambs during the first four months of life. Blood samples of 35 female lambs were collected at 30, 60, 90, and 120 days old to perform the complete blood count (CBC). The erythrocyte and leukocyte parameters, TPP, and fibrinogen were determined. The means for total red blood cell

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Syrogiannopoulos, George A., George H. McCracken, and John D. Nelson. "Osteoarticular Infections in Children With Sickle Cell Disease." Pediatrics 78, no. 6 (1986): 1090–96. http://dx.doi.org/10.1542/peds.78.6.1090.

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Thirteen children with sickle cell disease were identified as having 14 episodes of osteoarticular infection in a review of 27 years' experience. There were eight episodes of osteomyelitis or osteoarthritis and six of suppurative arthritis alone. The etiologic agents in osteomyelitis or osteoarthritis were Salmonella sp in four cases, Escherichia coli in one, Enterobacter aerogenes in one, Staphylococcus aureus in one, and Haemophilus influenzae type b in one. Five of the cases with infection limited to the joint were caused by Streptococcus pneumoniae; the sixth was caused by H influenzae typ

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Bitter, MA, ME Neilly, MM Le Beau, MG Pearson, and JD Rowley. "Rearrangements of chromosome 3 involving bands 3q21 and 3q26 are associated with normal or elevated platelet counts in acute nonlymphocytic leukemia." Blood 66, no. 6 (1985): 1362–70. http://dx.doi.org/10.1182/blood.v66.6.1362.1362.

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Abstract Fourteen patients with acute nonlymphocytic leukemia (ANLL) or dysmyelopoietic syndromes were found to have abnormalities involving the long arm of chromosome 3. In eight patients, the structural rearrangements involved both bands 3q21 and 3q26 and included t(3;3) (four patients), inv(3) (three patients), and ins(5;3) (one patient). Before treatment, seven of these eight patients had platelet counts above 100,000 per microliter, five had normal or elevated platelet counts, and four had significantly elevated platelet counts (600,000 to 1,731,000 per microliter). In each of the eight c

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Bitter, MA, ME Neilly, MM Le Beau, MG Pearson, and JD Rowley. "Rearrangements of chromosome 3 involving bands 3q21 and 3q26 are associated with normal or elevated platelet counts in acute nonlymphocytic leukemia." Blood 66, no. 6 (1985): 1362–70. http://dx.doi.org/10.1182/blood.v66.6.1362.bloodjournal6661362.

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Fourteen patients with acute nonlymphocytic leukemia (ANLL) or dysmyelopoietic syndromes were found to have abnormalities involving the long arm of chromosome 3. In eight patients, the structural rearrangements involved both bands 3q21 and 3q26 and included t(3;3) (four patients), inv(3) (three patients), and ins(5;3) (one patient). Before treatment, seven of these eight patients had platelet counts above 100,000 per microliter, five had normal or elevated platelet counts, and four had significantly elevated platelet counts (600,000 to 1,731,000 per microliter). In each of the eight cases, nor

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le van den Akker, Emi, Timothy J. Satchwell, Jo F. Flatt, et al. "Alterations in the band3/Rh Protein Complex in Erythrocyte Membranes of Protein 4.2 (Chartres) deficient Cells Occur Late during Erythropoiesis." Blood 112, no. 11 (2008): 3460. http://dx.doi.org/10.1182/blood.v112.11.3460.3460.

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Abstract We report on a 40 year old patient with mild hereditary spherocytosis (RBC: 4.43×1012/dL; Reticulocyte count: 253×109/dL; Hb: 14g/dL), whose red blood cells completely lack protein 4.2. Genetic analysis showed that the patient was a double heterozygote for EPB42 deletions; one allele lacked exon 9 but the sequence remained in frame (protein 4.2 Chartres I) and the other allele contained a di-nucleotide deletion resulting in a premature stop signal (protein 4.2 Chartres II). Homology modelling showed that the hairpin region that forms the proposed band 3 binding site is still present i

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Tarig Osman Khalafallah Ahmed, Ekhlas Alrasheid Abu Elfadul, Ahmed A. Agab Eldour, and Omer Ibrahim Abdallah Mohammed. "The prevalence of leukocyte abnormalities among Sudanese patients with sickle cell disease." World Journal of Advanced Research and Reviews 9, no. 1 (2021): 262–67. http://dx.doi.org/10.30574/wjarr.2021.9.1.0026.

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Sickle cell disease (SCD) is an inherited blood disorder that affects red blood cells. The study was conducted in Elobied town during the period May 2011 to September 2011. The aim of this study is to detect the abnormalities of leucocytes among sickle cell anemic patients. 40 sickle cell anemic patients; age range between 8 months to 23 years. Blood sample was taken for all patients and the laboratory investigation were performed using automated estimation for: hemoglobin (Hb), Packed cell volume (PCV), red cell count (RBCs), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell conce

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Tarig, Osman Khalafallah Ahmed, Alrasheid Abu Elfadul Ekhlas, A. Agab Eldour Ahmed, and Ibrahim Abdallah Mohammed Omer. "The prevalence of leukocyte abnormalities among Sudanese patients with sickle cell disease." World Journal of Advanced Research and Reviews 9, no. 1 (2021): 262–67. https://doi.org/10.5281/zenodo.4625453.

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Sickle cell disease (SCD) is an inherited blood disorder that affects red blood cells. The study was conducted in Elobied town during the period May 2011 to September 2011. The aim of this study is to detect the abnormalities of leucocytes among sickle cell anemic patients. 40 sickle cell anemic patients; age range between 8 months to 23 years. Blood sample was taken for all patients and the laboratory investigation were performed using automated estimation for: hemoglobin (Hb), Packed cell volume (PCV), red cell count (RBCs), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell conce

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Bonilla, Marta C., Leonie Fingerhut, Adriana Alfonso-Castro, et al. "How Long Does a Neutrophil Live?—The Effect of 24 h Whole Blood Storage on Neutrophil Functions in Pigs." Biomedicines 8, no. 8 (2020): 278. http://dx.doi.org/10.3390/biomedicines8080278.

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Neutrophils are important effector cells of the innate immune system, traditionally regarded to have a short life span. The goal of this study was to evaluate the effect of the whole blood storage on neutrophil functions, e.g., viability, antimicrobial effect, neutrophil extracellular trap (NET) formation and phagocytosis. Therefore, fresh porcine whole blood was compared to whole blood stored for 24 h in the dark at room temperature. Different cell parameters in whole blood and in isolated neutrophils were analyzed. The following parameters were analyzed: cell count, band and segmented neutro

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Martins, Christina Resende, Pablo Gomes Noleto, Renata Lima de Miranda, and Antonio Vicente Mundim. "Blood profile of obese and aged dogs (Canis familiaris)." Acta Veterinaria Brno 88, no. 1 (2019): 33–41. http://dx.doi.org/10.2754/avb201988010033.

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The aim of this study was to evaluate the blood profile and haematological changes in obese adult and senior dogs. An evaluation was made from the blood profile of 59 obese adult and senior dogs. The animals were divided into two groups, group 1 (G1) with 30 obese adult dogs (2 to 8 years) and group 2 (G2) with 29 obese senior dogs (over 8 years old). The mean values of the erythrogram and platelet count parameters of both groups remained within the compared reference ranges except the red cell distribution width (RDW), whose values were lower than the reference ranges in both groups. A compar

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Dass, Jasmita, Sudheer Arava, Pravas Chandra Mishra, Amit Kumar Dinda, and Hara Prasad Pati. "Role of CD138, CD56, and light chain immunohistochemistry in suspected and diagnosed plasma cell myeloma: A prospective study." South Asian Journal of Cancer 08, no. 01 (2019): 60–64. http://dx.doi.org/10.4103/sajc.sajc_64_17.

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Abstract Introduction: Plasma cells (PCs) have conventionally been counted on the bone marrow aspirate, and small focal involvement may be missed even on bone marrow biopsy sections. Material and Methods: We aimed to study the role of CD138, CD56, anti-κ, and anti-λ immunohistochemistry (IHC) to separate PC myeloma from reactive plasmacytosis and to study the utility of these in cases suspected as myelomas and lacking >10% PCs on bone marrow aspirate. The study comprised 35 diagnosed myelomas, 20 reactive plasmacytosis, and 19 M-band positive suspected myelomas. CD138 IHC was performed on a

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Adami, Dante, Sebastian Riechert, Tim Schmittmann, et al. "Autonomous Region-of-Interest Detection, Cell Segmentation and Cell Classification in Bone Marrow Cytomorphology Using Deep Learning." Blood 144, Supplement 1 (2024): 4975. https://doi.org/10.1182/blood-2024-205235.

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The differential nucleated cell count in bone marrow smears forms the diagnostic basis in hematological disorders. The current gold standard involves manually detecting evaluable regions of interest (ROI) and subjectively categorizing up to 500 cells to form a diagnosis. This process is time-consuming, labor-intensive, and suffers from high inter-observer variability. To address this issue, we propose an end-to-end deep learning pipeline for automating cytomorphology in bone marrow smears, covering ROI selection in whole slide images (WSI), cell detection and classification of 20 cell types. R

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Guimaraes, Ana M. S., Manoel L. Javorouski, Marcelo Bonat, et al. "Molecular detection of "Candidatus Mycoplasma haemominutum" in a lion (Panthera leo) from a brazilian zoological garden." Revista do Instituto de Medicina Tropical de São Paulo 49, no. 3 (2007): 195–96. http://dx.doi.org/10.1590/s0036-46652007000300011.

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Although Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" infections have been reported in wild cats from United States, their presence among native and captive wild cats in Brazil is still unknown. A 12 year old healthy male lion (Panthera leo) from the Zoological Garden of Curitiba, Brazil was anesthetized for transportation and dental evaluation. A blood sample was obtained for a complete blood cell count (CBC) and PCR analysis. DNA was extracted and fragments of Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" 16S ribosomal RNA gene were amplified in PCR assays.

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Cakmak, Ayse, Kader Yildiz, and Neslihan Sursal. "Neutrophil isolation from feline blood using discontinuous Percoll dilutions." Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere 46, no. 06 (2018): 399–402. http://dx.doi.org/10.1055/s-0038-1677404.

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Summary Objective: Some studies have performed in vitro neutrophil isolation from feline blood. The major limiting factor for these studies is the small volume of blood that can be collected without development of potentially life-threatening complications. In the present study we attempted neutrophil isolation from feline venous blood samples using discontinuous Percoll gradients. Material and methods: Blood was collected from the cephalic vein of clinically healthy adult cats. The blood samples were layered on Percoll dilutions (72 %, 63 %, 54 % and 45 %). After centrifugation, the feline po

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Athigakunagorn, Jadsada, Panupong Sukpan, and Teerasak Jiravongbunrod. "PS01.094: PREDICTING FACTOR OF FAILURE OF CONSERVATIVE TREATMENT AND OUTCOME IN PATIENTS WITH ACUTE CAUSTIC FOREGUT INJURY." Diseases of the Esophagus 31, Supplement_1 (2018): 76. http://dx.doi.org/10.1093/dote/doy089.ps01.094.

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Abstract Background Most of the patients with caustic injury can be successfully manage with conservative treatment. However, some patients with high severity required surgery. In this study, we assess failure rate, outcome and predictors of failure of conservative treatment in patients with acute caustic foregut injuries. Methods We retrospectively reviewed clinical records of patients with acute caustic foregut injuries who were hospitalized and treated at the Bhumibol Adulyadej Hospital from January2008 – December 2015. Analysis the data included the clinical factor, laboratory result, fail

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Wrzyszcz, Aneta, Joanna Urbaniak, Tomasz Wrobel, et al. "Plasma Gelatinolytic Activity in Leukemias." Blood 104, no. 11 (2004): 3823. http://dx.doi.org/10.1182/blood.v104.11.3823.3823.

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Abstract Gelatinase A (Matrix Metalloproteinase 2, MMP-2) and B (Matrix Metalloproteinase 9, MMP-9) are secreted by connective tissue, endothelial, epithelial and hematopoietic cells. Elastase and gelatinases produced by granulocytes are released in whole blood in response to varied stimuli. They induce breakdown of connective tissue and play an important role in processes of cell transmigration and tissue remodeling. The aims of this study were to determine and compare the plasma concentration of PMN elastase and the gelatinase activity in patients with chronic myelogenous leukemia (CML, n=16

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Sakhno, L. V., I. V. Koltuntceva, A. N. Rasteryaev, et al. "Complete blood count in healthy newborns in the first days of life (a comparative analysis against the 20th-century norms): A retrospective cohort study." Kuban Scientific Medical Bulletin 32, no. 1 (2025): 17–28. https://doi.org/10.25207/1608-6228-2025-32-1-17-28.

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Complete blood count is one of the most accessible and common laboratory tests available today. In present-day healthy newborns, complete blood count levels differ from the normative values used in the 1970s, which may require critical analysis, clarification, further research, and revision of the concept of norm.Objectives. To identify changes in the hematologic parameters of healthy newborns born in 2022 as compared to those reported in the 1930s and 1967–1970s.Methods. A retrospective cohort study of neonatal records was conducted at the Neonatal Physiology Unit of the Perinatal Center of t

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Gawoski, John M., and Winnie W. Ooi. "Dengue Fever Mimicking Plasma Cell Leukemia." Archives of Pathology & Laboratory Medicine 127, no. 8 (2003): 1026–27. http://dx.doi.org/10.5858/2003-127-1026-dfmpcl.

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Abstract Extreme plasmacytosis in peripheral blood is a rare finding most often associated with plasma cell leukemia but rarely with other malignancies, infectious diseases, or drug reactions. We report the case of a 40-year-old man who was a US expatriate working and traveling in East Asia. He presented with complaints of fever, myalgia, headache, vomiting, and diarrhea of 3 days' duration. An initial evaluation revealed elevated liver function tests, thrombocytopenia (68 × 103/μL), and a white blood cell count of 5.8 × 103/μL with 19% plasma cells (1100/μL), 9% abnormal plasmacytoid lymphocy

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Desdiani, Desdiani, Iris Rengganis, Samsuridjal Djauzi, et al. "Fibropreventive and Antifibrotic Effects of Uncaria gambir on Rats with Pulmonary Fibrosis." Evidence-Based Complementary and Alternative Medicine 2022 (June 26, 2022): 1–11. http://dx.doi.org/10.1155/2022/6721958.

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Pulmonary fibrosis causes scar tissue formation that disrupts the functioning of the lungs. Uncaria gambir (Hunter) Roxb (hereafter gambir)—a plant native to West Sumatra in Indonesia—contains flavonoid (+)-catechin, which has strong antioxidant activity and can be used to combat pulmonary fibrosis. This random in vivo experimental study analyzed the antifibrotic effect of gambir on the lungs of rats with bleomycin-induced fibrosis. The subjects were 10 groups of 10-week-old male rats weighing around 200–250 g. All groups were terminated at the end of the seventh week or on day 50. The lungs w

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Bjornsson, Sven, Saga Wahlstrom, Ingela Bernevi, and Per Simonsson. "A Single Tube Antibody Panel for Differential Count by Flow Cytometry." Blood 106, no. 11 (2005): 4938. http://dx.doi.org/10.1182/blood.v106.11.4938.4938.

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Abstract Introduction Several techniques are used for classification of leukocytes, i.e. microscopy, cell counters and flow cytometry. A skilled microscopist will classify at least 14 cell classes in bone marrow or blood. We have designed a one tube immunophenotyping panel for leukocyte classification and validated it against microscopy. Multiparameter flow cytometry provide at least seven measurements on each cell passing through the laser beam. Our goal was to use the seven parameters available in our flow cytometer to provide a reliable differential count using only one tube. Materials and

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Mattè, Alessandro, Wilson Anand, Enrica Federti, et al. "The Pyruvate Kinase Activator Mitapivat Ameliorates Anemia and Prevents Iron Overload in a Mouse Model of Hereditary Spherocytosis." Blood 136, Supplement 1 (2020): 29. http://dx.doi.org/10.1182/blood-2020-142232.

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Hereditary spherocytosis (HS) is the most common cause of inherited red cell membranopathy, due to mutations in genes encoding for membrane or cytoskeletal proteins, including band 3, ankyrin, spectrin, band 4.1 or band 4.2. Membrane instability results in membrane surface area loss and generation of spherocytic red cells with elevated MCHC, decreased cellular deformability and reduced red cell survival, due to splenic sequestration. Clinical management of the hemolytic anemia due to HS depends on the age of the patient and the severity of anemia. Splenectomy is indicated in children with symp

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Rahman, M., F. Huq Ronny, and H. Islam. "A Case of Ambiguous Lineage Acute Leukemia Posing Diagnostic Challenge." American Journal of Clinical Pathology 158, Supplement_1 (2022): S104—S105. http://dx.doi.org/10.1093/ajcp/aqac126.220.

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Abstract Introduction/Objective N/A. Methods/Case Report Acute leukemias with ambiguous lineage encompass those leukemias that show no clear evidence of differentiation along a single lineage and account for less than 4% of all cases of acute leukemias and often poses diagnostic dilemma. We present a challenging case of an ambiguous lineage acute leukemia in a previously healthy 27-year-old male, who presented to the ED with acute fever, dyspnea on exertion, nausea and vomiting. A complete blood count (CBC) showed a white blood cell count of 323 × 109/L, Hemoglobin of 3.3 g/dl, Platelet count

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Peters, Luanne L., Rebecca A. Swearingen, Sabra G. Andersen, et al. "Identification of quantitative trait loci that modify the severity of hereditary spherocytosis in wan, a new mouse model of band-3 deficiency." Blood 103, no. 8 (2004): 3233–40. http://dx.doi.org/10.1182/blood-2003-08-2813.

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Abstract Defects in red blood cell (RBC) membrane skeleton components cause hereditary spherocytosis (HS). Clinically, HS varies significantly even among individuals with identical gene defects, illustrating the profound effects of genetic background on disease severity. We exploited a new spontaneous mouse model, wan, which arose on the inbred C3H/HeJ strain, to identify quantitative trait loci (QTL) that modify the HS phenotype. Homozygous wan mice have severe HS due to a complete deficiency of erythroid band 3. A QTL analysis of RBC count, hemoglobin, hematocrit, mean corpuscular volume (MC

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Revel-Vilk, Shoshana, Ela Shai, Nivin Gashem, et al. "New Mutation in Chromosome 9 Associated with Familial Autosomal Recessive Macrothrombocytopenia." Blood 124, no. 21 (2014): 2776. http://dx.doi.org/10.1182/blood.v124.21.2776.2776.

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Abstract We report a family with new autosomal recessive disorder associated with macrothrombocytopenia which is different from all reported hereditary macrothrombocytopenia. Case report: The affected family members report mild bruising and epistaxis. The females’ family members report, in addition, severe menorrhagia and recurrent episodes of hemorrhagic corpus luteum. Two family members with macrothrombocytopenia and two without macrothrombocytopenia report hearing loss and cataracts. Methods: The study protocol was approved by our local Ethics committee and written consent was obtained to p

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Shah, Shuchi, Preston R. Miller, J. Wayne Meredith, and Michael C. Chang. "Elevated Admission White Blood Cell Count in Pregnant Trauma Patients: An Indicator of Ongoing Placental Abruption." American Surgeon 68, no. 7 (2002): 644–47. http://dx.doi.org/10.1177/000313480206800719.

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Placental abruption (PA) is a common cause of fetal demise in pregnant trauma patients. Diagnosis of PA may be difficult, and multiple diagnostic clues are currently used including uterine/ abdominal pain and tenderness, bleeding, maternal hemodynamic instability, and evidence of PA by ultrasonography or other fetal monitoring. Although diagnosis may be problematic fetal and maternal survival are dependent on early diagnosis and intervention. The purpose of this study is to determine predictive factors present at admission associated with PA in trauma victims. Records of all pregnant trauma pa

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MOLMERET, M., S. JARRAUD, J. PIERRE MORIN, et al. "Different growth rates in amoeba of genotypically related environmental and clinical Legionella pneumophila strains isolated from a thermal spa." Epidemiology and Infection 126, no. 2 (2001): 231–39. http://dx.doi.org/10.1017/s0950268801005258.

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Two cases of legionellosis occurring 3 years apart were acquired in the same French thermal spa and were apparently due to the same strain of Legionella pneumophila serogroup 1, as shown by genomic macrorestriction analysis. Minor differences between the two isolates were found by random amplification PCR profiling which showed an additional band with one of the isolates. Analysis of 107 L. pneumophila strains isolated from the spa waters by genome macrorestriction failed to identify the infective strain, but a closely related L. pneumophila serogroup 3 strain differing from the clinical isola

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