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Journal articles on the topic 'Barcoding gene'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Odah, Mohammad Ahmad Ahmad. "Unlocking the genetic code: Exploring the potential of DNA barcoding for biodiversity assessment." AIMS Molecular Science 10, no. 4 (2023): 263–94. http://dx.doi.org/10.3934/molsci.2023016.

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<abstract> <p>DNA barcoding is a crucial method for assessing and monitoring species diversity amidst escalating threats to global biodiversity. I explore DNA barcoding's potential as a robust and reliable tool for biodiversity assessment. It begins with a comprehensive review of existing literature, delving into the theoretical foundations, methodologies and applications of DNA barcoding. The suitability of various DNA regions, like the COI gene, as universal barcodes are extensively investigated. Additionally, the advantages and limitations of different DNA sequencing technologie

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SUSANTI, R., RETNO SRI ISWARI, FIDIA FIBRIANA, and INDRIAWATI INDRIAWATI. "The duck cytochrome oxidase I (COI) gene: Sequence and patterns analysis for potential barcoding tool." Biodiversitas Journal of Biological Diversity 19, no. 3 (2018): 997–1003. http://dx.doi.org/10.13057/biodiv/d190331.

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Susanti R, Iswari RS, Fibriana F, Indriawati. 2018. The duck cytochrome oxidase I (COI) gene: Sequence and patterns analysis for potential barcoding tool. Biodiversitas 19: 997-1003. The local duck DNA barcoding is still rarely conducted in Indonesia while DNA barcoding is extensively used as a tool of species identification and delineation tool. This study aimed to analyze the sequence and patterns of Central Javanese ducks mitochondrial cytochrome c oxidase subunit 1 (COI) gene. Feather samples of seven breeds of native duck were collected from traditional husbandries in Central Java. The sa

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Bezeng, B. S., T. J. Davies, B. H. Daru, et al. "Ten years of barcoding at the African Centre for DNA Barcoding." Genome 60, no. 7 (2017): 629–38. http://dx.doi.org/10.1139/gen-2016-0198.

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The African Centre for DNA Barcoding (ACDB) was established in 2005 as part of a global initiative to accurately and rapidly survey biodiversity using short DNA sequences. The mitochondrial cytochrome c oxidase 1 gene (CO1) was rapidly adopted as the de facto barcode for animals. Following the evaluation of several candidate loci for plants, the Plant Working Group of the Consortium for the Barcoding of Life in 2009 recommended that two plastid genes, rbcLa and matK, be adopted as core DNA barcodes for terrestrial plants. To date, numerous studies continue to test the discriminatory power of t

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Saputri, R., A. Arsyadi, F. S. Valen, and W. Wahyuni. "Molecular identification of Pelawan Merah (Tristaniopsis merguensis Griff.) as an endemic plant from Bangka Belitung Island using chloroplast DNA rbcL gene." IOP Conference Series: Earth and Environmental Science 1267, no. 1 (2023): 012065. http://dx.doi.org/10.1088/1755-1315/1267/1/012065.

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Abstract The DNA barcoding from this molecular identification will be submitted to the NCBI Genbank database to become a standard in the identification of Tristaniopsis merguensis Griff based on the rbcL gene analysis. This research will be used as a crucial information to identify the endemic species of Tristaniopsis merguensis Griff. which has no conservation status yet on IUCN Red List & Threated species. The stages of this research were collecting samples of Pelawan Merah leaves from Hutan Pelawan Namang, Central Bangka Regency. Molecular analysis was carried out to obtain the marker g

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Kolondam, Beivy J. "Evaluation of 16S rRNA Gene Sequence for DNA Barcoding of Tuna Fish." Jurnal Ilmiah PLATAX 10, no. 1 (2022): 108. http://dx.doi.org/10.35800/jip.v10i1.38861.

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For fish product authentication, DNA barcoding has been a reliable tool. This is due to its requirement of a small amount of tissue sample in order to conduct a full analysis for species identification. This research aimed to conduct an assessment for the use of 16S rRNA gene sequence for tuna fish identification through DNA barcoding. Previous in silico studies using the COI gene and CYB gene were conducted using the same tuna fish specimens. A comparison of 16S rRNA gene sequence between Bluefin tuna (five species), Yellowfin tuna (three species), and another group of tuna (five species) sho

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McFADDEN, CATHERINE S., YEHUDA BENAYAHU, ERIC PANTE, JANA N. THOMA, P. ANDREW NEVAREZ, and SCOTT C. FRANCE. "Limitations of mitochondrial gene barcoding in Octocorallia." Molecular Ecology Resources 11, no. 1 (2010): 19–31. http://dx.doi.org/10.1111/j.1755-0998.2010.02875.x.

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Li, Xiwen, Yang Yang, Robert J. Henry, Maurizio Rossetto, Yitao Wang, and Shilin Chen. "Plant DNA barcoding: from gene to genome." Biological Reviews 90, no. 1 (2014): 157–66. http://dx.doi.org/10.1111/brv.12104.

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HUTCHINSON, RACHEL, and JAMIE R. STEVENS. "Barcoding in trypanosomes." Parasitology 145, no. 5 (2017): 563–73. http://dx.doi.org/10.1017/s0031182017002049.

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SUMMARYTrypanosomes (genus Trypanosoma) are parasites of humans, and wild and domestic mammals, in which they cause several economically and socially important diseases, including sleeping sickness in Africa and Chagas disease in the Americas. Despite the development of numerous molecular diagnostics and increasing awareness of the importance of these neglected parasites, there is currently no universal genetic barcoding marker available for trypanosomes. In this review we provide an overview of the methods used for trypanosome detection and identification, discuss the potential application of

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Mallo, Diego, and David Posada. "Multilocus inference of species trees and DNA barcoding." Philosophical Transactions of the Royal Society B: Biological Sciences 371, no. 1702 (2016): 20150335. http://dx.doi.org/10.1098/rstb.2015.0335.

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The unprecedented amount of data resulting from next-generation sequencing has opened a new era in phylogenetic estimation. Although large datasets should, in theory, increase phylogenetic resolution, massive, multilocus datasets have uncovered a great deal of phylogenetic incongruence among different genomic regions, due both to stochastic error and to the action of different evolutionary process such as incomplete lineage sorting, gene duplication and loss and horizontal gene transfer. This incongruence violates one of the fundamental assumptions of the DNA barcoding approach, which assumes

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10

EBACH, MALTE C. "Taxonomy and the DNA Barcoding Enterprise." Zootaxa 2742, no. 1 (2011): 67. http://dx.doi.org/10.11646/zootaxa.2742.1.5.

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DNA Barcoding is elusive to many taxonomists. Like the numbers in a barcode, barcoding attempts to link a type specimen with a part of its DNA, most commonly from the mitochondrial Cytochrome c Oxidase subunit I (COI) gene.

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Ali Alshehri, Mohammed, Al Thabiani Aziz, Othman Alzahrani, et al. "DNA-barcoding and Species Identification for some Saudi Arabia Seaweeds using rbcL Gene." Journal of Pure and Applied Microbiology 13, no. 4 (2019): 2035–44. http://dx.doi.org/10.22207/jpam.13.4.15.

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Javaheri, Tehrani Sahar, Elham Rezazadeh, Kakhki Niloofar Alaei, et al. "DNA barcoding of passerine birds in Iran." ZooKeys 1236 (April 24, 2025): 19–39. https://doi.org/10.3897/zookeys.1236.143336.

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Exploring genetic diversity is essential for precise species delimitation, especially within taxonomically complex groups like passerine birds. Traditional morphological methods often fail to resolve species boundaries; however, DNA barcoding, particularly through the mitochondrial cytochrome c oxidase subunit I (<i>COI</i>) gene, provides a powerful complementary method for accurate species identification. This study establishes a comprehensive DNA barcode library for Iranian passerine birds, analyzing 546 <i>COI</i> sequences from 94 species across 23 families and 53 genera. There is a prono

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Alkathiri, Badriah, Subin Lee, KyuSung Ahn, et al. "DNA Barcoding Using 18S rRNA Gene Fragments for Identification of Tick-Borne Protists in Ticks in the Republic of Korea." Pathogens 13, no. 11 (2024): 941. http://dx.doi.org/10.3390/pathogens13110941.

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The objective of this study was to evaluate the diversity and prevalence of tick-borne protists in the Republic of Korea via DNA barcoding using 18S rRNA gene fragments and PCR. Between 2021 and 2022, questing ticks were collected using the flagging method, with a total of 13,375 ticks collected and pooled into 1003 samples. Of these, 50 tick pools were selected for DNA barcoding targeting the V4 and V9 regions of 18S rRNA using the MiSeq platform. A taxonomic analysis of the amplicon sequence variants identified three genera of protozoa, namely Hepatozoon canis, Theileria luwenshuni, and Greg

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Fukunaga, Nao, Moe Shimizu, Shinnosuke Teruya, et al. "Reassessment of the mitochondrial 12S-rRNA gene for DNA barcoding of museum specimens of shelled marine gastropods from Japan." E3S Web of Conferences 322 (2021): 01028. http://dx.doi.org/10.1051/e3sconf/202132201028.

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DNA barcoding is an effective and powerful tool for taxonomic identification and thus very useful for biodiversity monitoring. This study investigated the usefulness of the mitochondrial 12S-rRNA gene for the DNA barcoding of shelled marine gastropods. To do so, we determined partial 12S-rRNA sequences of 75 vouchered museum specimens from 69 species of shelled gastropods from Japan. The specimens have been identified morphologically, and natural history data catalog. Sequence analyses through BLAST searches, maximum likelihood phylogenetic analysis, and species delimitation analysis suggested

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Kolondam, Beivy J. "Variasi Sekuens Gen COI untuk DNA Barcoding Ikan Tuna." Media Teknologi Hasil Perikanan 8, no. 2 (2020): 70. http://dx.doi.org/10.35800/mthp.8.2.2020.28378.

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DNA barcoding has been used for species identification of fishes, especially for fish product authentication. In tuna fish food products authentication, DNA barcoding is needed due to its requirement of small amount of samples for species identification. The COI gene, located in mitochondria of animal cells, is established as standard marker for animal DNA barcoding. This research aimed to study the variation in COI gene of tuna fish species in three groups, such as Bluefin tuna (five species), Yellowfin tuna (three species), and other tuna species (five species). The variation comparison show

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Farhana Ramli, Farah, Kaviarasu Munian, Nursyuhada Othman, et al. "A comparative assessment of 16S ribosomal RNA and Cytochrome C Oxidase Subunit I (COI) Primers for Amphibian DNA Barcoding." BIO Web of Conferences 94 (2024): 01003. http://dx.doi.org/10.1051/bioconf/20249401003.

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Amphibians, a diverse and ecologically important group, are facing global declines due to various factors, including habitat loss and climate change. Accurate species identification is crucial for effective conservation efforts, and DNA barcoding has emerged as a powerful tool in this regard. This study compares the efficacy of two DNA barcoding primer sets, targeting the 16S ribosomal RNA gene and the Cytochrome Oxidase I (COI) gene, for identifying 20 amphibian species. While both primer sets successfully amplified sequences, the 16S rRNA gene region identified all 20 samples, whereas the CO

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Powell, Robyn F., Anthony R. Magee, and James S. Boatwright. "Decoding ice plants: challenges associated with barcoding and phylogenetics in the diverse succulent family Aizoaceae." Genome 61, no. 11 (2018): 815–21. http://dx.doi.org/10.1139/gen-2018-0055.

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Aizoaceae is the largest succulent plant family in the world, including in excess of 1800 species. Despite its richness, a large proportion of its taxa are listed as data deficient and as such, has been identified as the top priority for taxonomic research in South Africa. Limitations to accurate taxonomic identification of taxa in the family may be partly attributed to the degree of technical knowledge required to identify taxa in the Aizoaceae. DNA barcoding may provide an alternative method of identification; however, the suitability of commonly used gene regions has not been tested in the

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IMTIAZ, AYESHA, SITI AZIZAH MOHD NOR, and DARLINA MD NAIM. "Review: Progress and potential of DNA barcoding for species identification of fish species." Biodiversitas Journal of Biological Diversity 18, no. 4 (2017): 1394–405. http://dx.doi.org/10.13057/biodiv/d180415.

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Imtiaz A, Mohd Nor SA, Md. Naim D. 2017. Review: Progress and potential of DNA barcoding for species identification of fish species. Biodiversitas 18: 1394-1405. DNA barcoding is a molecular technique to identify species by utilizing 600-800 base pairs genetic primer segments of mitochondrial gene cytochrome oxidase I. DNA barcoding has high potential to identify species into taxa, resolves ambiguousness in species identification, helps in accurate species identification, categorize species for conservation and also communize the information in the form of database system. The main challenge t

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Aykut, Yilmaz. "The importance in DNA barcoding of the regions which is covering rRNA genes and its sequences in the genus Quercus L." Bangladesh Journal of Plant Taxonomy 27, no. 2 (2020): 261–71. http://dx.doi.org/10.3329/bjpt.v27i2.50666.

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Turkey with 18 oak (Quercus) species is one of the richest country according to species number and diversity. The most important reason of the species diversity in Turkey is its location and geomorphological structure which increase climatic effects and seperate Turkey into different phytogeographic regions. Furthermore, hybridization behaviours which frequently observed between oak species, genetic drift, gene flow and ecological factors cause morphological variations in the plants species. All of these factors make it difficult to define the species concept for plant groups like oaks. Theref

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Cacciali, Pier, Emilio Buongermini, and Gunther Köhler. "Barcoding Analysis of Paraguayan Squamata." Diversity 11, no. 9 (2019): 152. http://dx.doi.org/10.3390/d11090152.

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Paraguay is a key spot in the central region of South America where several ecoregions converge. Its fauna (and specifically its herpetofauna) is getting better studied than years before, but still there is a lack of information regarding molecular genetics, and barcoding analyses have proven to be an excellent tool in this matter. Here, we present results of a barcoding analysis based on 16S rRNA gene sequences, providing valuable data for the scientific community in the region. We based our fieldwork in several areas of Paraguay. We analyzed 249 samples (142 sequenced by us) with a final ali

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Liu, Qing-Hua, Li-Yun Jiang, and Ge-Xia Qiao. "DNA barcoding of Greenideinae (Hemiptera : Aphididae) with resolving taxonomy problems." Invertebrate Systematics 27, no. 4 (2013): 428. http://dx.doi.org/10.1071/is13014.

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Species of the Greenideinae are distributed mainly throughout South-east Asia and include some important agricultural and horticultural pests. Rapid and accurate species circumscription and identification in this subfamily are very difficult because similar morphological traits are shared among congeneric species. Here, we test the efficiency of DNA barcoding in the Greenideinae by analysing 214 samples covering 42 species belonging to nine genera using two mitochondrial gene fragments (COI barcode fragment and Cytb gene fragment). The results show that DNA barcoding is a useful species identi

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Adamu, Abdulrahman, Abdulmumin Abubakar, Adamu Ambi, Najma Ilyas, and Mujtaba Abubakar. "DNA Barcoding of Clerodendrum capitatum Using rbcL Gene." Tropical Journal of Natural Product Research 4, no. 4 (2020): 123–30. http://dx.doi.org/10.26538/tjnpr/v4i4.2.

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Fan, H. Christina, Glenn K. Fu, and Stephen P. A. Fodor. "Combinatorial labeling of single cells for gene expression cytometry." Science 347, no. 6222 (2015): 1258367. http://dx.doi.org/10.1126/science.1258367.

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We present a technically simple approach for gene expression cytometry combining next-generation sequencing with stochastic barcoding of single cells. A combinatorial library of beads bearing cell- and molecular-barcoding capture probes is used to uniquely label transcripts and reconstruct the digital gene expression profile of thousands of individual cells in a single experiment without the need for robotics or automation. We applied the technology to dissect the human hematopoietic system and to characterize heterogeneous response to in vitro stimulation. High sensitivity is demonstrated by

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Valiyaparambil, Jisha Jacob, Anisha S. Menon, Manoj Mathews, and Nandini N J. "DNA Barcoding of Fish Fauna using Mitochondrial CO1 Gene." Mapana Journal of Sciences 23, no. 1 (2024): 135–49. https://doi.org/10.12723/mjs.68.7.

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This study aimed to investigate the quantitative relationships between four fish species from three genera based on molecular analyses (barcoding) of nine species from five genera utilizing the mitochondrial COI gene. Species within the same genus showed more transitional incompatibilities than transversional mismatches. The samples were divided into four main groups by a phylogenetic tree built from the sequencing data (cytochrome COI) of samples from the two populations using the neighbour-joining method. As dissimilar species were clustered under separate nodes and similar species were clus

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Swagata, Sarkar. "DNA Barcoding: A New Tool for Solving Problems Related To Fungi Identification." Recent Trends in Pharmaceutical Sciences and Research 1, no. 1 (2019): 31–34. https://doi.org/10.5281/zenodo.2531065.

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DNA barcoding is a new term introduced in scientific literatures to identify organisms up to the species level. Nowadays, DNA barcoding has been widely used to identify fungi also. Moreover, DNA barcoding has huge utilities to verify the taxonomic identity of fungi. By using DNA barcoding of nuclear ribosomal internal transcribed spacer (ITS) of the rRNA gene with fungal specific ITS primers, ITS barcodes were generated. After generating they were sequenced and given for BLAST search in GenBank. The homologous sequences of the ITS region of fungi were found out and their relationship is being

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Ngui, Romano, Veeranoot Nissapatorn, and Guo-Jie Brandon-Mong. "Prospects on the Application of DNA Barcoding on Soil-Transmitted Helminths in Children." Journal of Pediatric Infectious Diseases 12, no. 04 (2017): 256–63. http://dx.doi.org/10.1055/s-0037-1603561.

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AbstractSince more than a decade, DNA barcoding has been widely used to examine biological samples and differentiate species, as well as employed in ecological and conservational studies. There is a growing interest of DNA barcoding, particularly in medical parasitology, but its potential utility in soil-transmitted helminths (STHs) remains unclear. Therefore, in this article, we review the studies using DNA barcoding and its applications in medical parasitology with special focus on STHs such as Ascaris lumbricoides, Ancylostoma duodenale, Necator americanus, and Trichuris trichiura. DNA barc

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Tabassum, Mehnus, Hawa Jahan, and Gulshan Ara Latifa. "Molecular identification of two gobi fishes of Bangladesh using cytochrome oxidase subunit I (CoI) gene sequences." Bangladesh Journal of Zoology 44, no. 2 (2017): 175–84. http://dx.doi.org/10.3329/bjz.v44i2.32757.

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DNA barcoding has been proposed as a means of quick species identification using a short standardized segment of DNA. Two species (Eleotris fusca and Glossogobius giuris) from the family Gobiidae and Eleotridae were selected for DNA barcoding using samples collected from different regions of Bangladesh. Cytochrome Oxidase Subunit I (COI) gene was sequenced from two different gobi fishes and compared with two previously published similar sequences from the genera Eleotris and Glossogobius. Multiple sequence alignment and the molecular systematic study were performed. The DNA barcode technique i

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ŞAHİN NEGİŞ, İnci. "Exploration of ITS region as DNA barcode for Kakothrips priesneri Pelikan phylogeny." Mediterranean Agricultural Sciences 36, no. 3 (2023): 123–27. http://dx.doi.org/10.29136/mediterranean.1344488.

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The Thripidae family is a diverse group of insects with global distribution and significant economic importance as pests of agricultural crops. Accurate identification and classification of Thripidae species are critical for their effective management and control. To aid in this effort, DNA barcoding using the ITS gene region has proven to be an efficient and reliable tool for identifying and differentiating Thripidae species. The high variability rate of the ITS region makes it particularly effective for identifying and classifying closely related species, providing valuable insight for under

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Rawal, Deepak. "Identification and confirmation of Chironomus circumdatus Kieffer, 1916 (Diptera: Chironomidae) using DNA Barcoding." International Journal of Zoological Investigations 7, no. 1 (2021): 301–7. https://doi.org/10.5281/zenodo.7598534.

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Present study was performed to identify and confirm collected larval Chironomid up to species level. To assess molecular taxonomy and phylogeny, DNA barcoding was done using Sanger dideoxy sequencing of mitochondrial cytochrome c oxidase subunit I (COI) gene of larval sample. Bioinformatics analysis was done by using NCBI’s BLAST software. By evidence of DNA barcoding, it was confirmed that present larval chironomid species was Chironomus circumdatus Kieffer, 1916.

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BILGIN, R., M. A. UTKAN, E. KALKAN, S. U. KARHAN, and M. BEKBÖLET. "DNA barcoding of twelve shrimp species (Crustacea: Decapoda) from Turkish seas reveals cryptic diversity." Mediterranean Marine Science 16, no. 1 (2014): 36. http://dx.doi.org/10.12681/mms.548.

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DNA barcoding is a useful tool for the identification and potential discovery of new species. In this study, DNA barcoding was employed by sequencing the mitochondrial cytochrome oxidase subunit I gene (COI) to characterize the genetic diversity of 12 shrimp species inhabiting Turkish coastal waters and, when possible, to compare with the genetic data available from different parts of the Mediterranean and eastern Atlantic. This study also comprises the first DNA barcoding study performed in the Turkish Seas using COI. A total of 40 shrimp specimens were collected and analyzed from 9 sites. Ge

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J. S., Rupali, Vidya Madhuri E., Gundreddy Raja Reddy, Voodikala S. Akhil, Ramya N., and Sagar D. "DNA Barcoding: Accelerating Insect Species Discovery and Biodiversity Documentation." Journal of Advances in Biology & Biotechnology 27, no. 7 (2024): 709–20. http://dx.doi.org/10.9734/jabb/2024/v27i71030.

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Species identification is essential for recognizing and describing biodiversity. Traditionally, this process has relied on morphological diagnosis through taxonomic studies, which have certain constraints such as subjectivity and time-consuming processes. With the advancement of modern molecular techniques, DNA barcoding has gained global attention. The term "DNA barcoding" refers to the technique of establishing species-level identification by sequencing a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene, the "DNA barcode," from a specimen that is taxonomically un

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G., Jayaraj. "A NOTE ON ITS AND DNA BARCODING -ITS IMPORTANCE IN SPECIES PHYLOGENETICS." INTERNATIONAL EDUCATIONAL JOURNAL OF SCIENCE AND ENGINEERING - IEJSE 7, no. 2 (2024): 09–20. https://doi.org/10.5281/zenodo.15606932.

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ITS sequence data in the present research has provided some useful insights resolving phylogenetic relationship among Eucalyptus species. It is to be complimented with rbcL, MatK and trnH-psbA gene study. There are 77 SNPs in an average in ITS fragment of 746 bps. Therefore, it contains highest percentage of variable characters in the gene. This study has shown a good distance between E. mitchelliana and the rest of species. The position of E. globulus and E. nitens is not clear. There is an overlap about E.dives and E.pauciflora. They are mixed up in E. stellulata, E. moorei and affin moorei

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Barbosa, Valmir C. "Information-theoretic signatures of biodiversity in the barcoding gene." Journal of Theoretical Biology 451 (August 2018): 111–16. http://dx.doi.org/10.1016/j.jtbi.2018.05.008.

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Muhammad Usman, Sahrish Ghazanfar, Sana Ashraf, et al. "Decoding Genetic Signatures for Identification: A Molecular Investigation Approach in Pakistan via Cytochrome C Oxidase I (COI) Gene Sequencing." FRONTIERS IN CHEMICAL SCIENCES 5, no. 1 (2024): 12–17. http://dx.doi.org/10.52700/fcs.v5i1.73.

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DNA barcoding is an efficient and rapid approach to the identification of species. A standard genetic marker is used to barcode the sequence of unidentified species. So, mitochondrial cytochrome c oxidase I (COI) genetic marker is mostly used for species molecular identification. Therefore, molecular identification and phylogeographic relationship of Cirrhinus reba (C. reba) were carried out in the present study. In the present study, we propose the barcoding analysis of the mitochondrial cytochrome c oxidase I (COI) gene of freshwater fish as different sequences were obtained from the sequenc

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Adhikari, Debraj, Resham B. Thapa, Samudra L. Joshi, et al. "Molecular identification of Chinese citrus fly, Bactrocera minax (Diptera: Tephritidae) in Nepal." Nepal Journal of Science and Technology 22, no. 1 (2023): 22–28. http://dx.doi.org/10.3126/njst.v22i1.67158.

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An accurate identification at the species level is often the first step in successfully controlling, mitigating and managing of insect pests. Species identification utilizing molecular approaches can complement morphological identification, often resulting more accurate result. Tephritid fruit fly insects can be identified quickly using DNA barcoding technology. In this study, Chinese fruit fly (Bactrocera minax), a destructive citrus pest collected in Nepal, was identified using barcoding method with the sequence of mitochondrial cytochrome c oxidase I (COI) gene.

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Rizwan, Syed, Hakim Abdul Bari, Arshad Saleem, Song Jingyuan, Xin Tianyi, and Muhammad Jahanzeb. "DNA Barcoding Based Identification of Rosa x Damascene and Prunus dulcis Herbs Using ITS2 Barcoding Gene Amplification." Journal of Applied Research in Plant Sciences 5, no. 01 (2023): 58–62. http://dx.doi.org/10.38211/joarps.2024.05.219.

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The considerable risk of adulteration in the herbs has raised commercial interest in the identification of medicinal herbs globally. DNA barcoding is the primary techniques for identifying the herbs at genetic level. This technique's key benefit is that it can identify the material's purity. This study focuses on the accurate identification of species utilizing Polymerase chain reaction-based nuclear universal internal transcribed spacer region (a barcode region) amplification and sequencing in 2 medically significant plants (Rosa damascene and Prunus dulcis) procured from the local herbal mar

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Ariyanti, Yanti, Ika Rini, Indah Oktaviani, and Sovia Leksikowati. "DNA Barcoding for Selected Mangrove-Based Estuary Fishes from Way Kambas National Park, Lampung Province, Indonesia." Journal of Tropical Life Science 11, no. 2 (2021): 151–60. http://dx.doi.org/10.11594/jtls.11.02.04.

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Over the past decade, DNA barcoding has provided new insight into fish ecology and biosystematics and led to new species' discovery. DNA barcoding is a method for the recognition and identification of species using short, standardised DNA fragments. The correct taxonomic identification of species is critical for the assessment and monitoring of biodiversity. This study applied DNA barcoding techniques to identify selected fish species from a mangrove-based estuary in Way Kambas National Park, Lampung Province, Indonesia. The gene encoding cytochrome c oxidase subunit I (COI) was amplified and

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Nuryanto, Agus, Kusbiyanto Kusbiyanto, and Dian Bhagawati. "Molecular barcoding of marine ornamental fish from the southern coast of West Java validates conventional identification." E3S Web of Conferences 322 (2021): 01004. http://dx.doi.org/10.1051/e3sconf/202132201004.

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Conventional identification of marine ornamental fish has faced difficulties due to similar color patterns of closed related species, or juvenile individuals have different color patterns from adult individuals. Molecular barcoding using the cytochrome c oxidase I (COI) gene provides a reliable tool for unmasking such difficulties. This study aimed to barcode marine ornamental fish from the southern coast of West Java. Fragment of the COI gene was sequenced from 54 morphotypes. In this study, we determined the taxonomic status of the samples based on a 5% genetic divergence, with the parameter

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Kúdelová, Tatiana, Samuel Krčmárik, Ivona Lužáková, Bibiana Bujačková, Karin Matická, and Matúš Kúdela. "DNA Barcoding of Black Flies (Diptera: Simuliidae) in Slovakia and Its Utility for Species Identification." Diversity 15, no. 5 (2023): 661. http://dx.doi.org/10.3390/d15050661.

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DNA barcoding based on the cytochrome oxidase I gene is increasingly used in black flies (Diptera: Simuliidae), but extensive data for larger areas are still rare. Slovakia, with well-explored black fly fauna, was chosen to verify the reliability of DNA barcoding for species identification. The DNA barcoding region of the COI gene of 235 individuals of 25 black fly species from Slovakia was sequenced. Among them, 30 sequence clusters with assigned Barcode Index Numbers (BINs) were identified, and 5 of them were recorded for the first time. The average intraspecific genetic divergence was 0–3.2

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Gong, Shaohua, Yanfei Ding, Yi Wang, Guangze Jiang, and Cheng Zhu. "Advances in DNA Barcoding of Toxic Marine Organisms." International Journal of Molecular Sciences 19, no. 10 (2018): 2931. http://dx.doi.org/10.3390/ijms19102931.

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There are more than 200,000 marine species worldwide. These include many important economic species, such as large yellow croaker, ribbonfish, tuna, and salmon, but also many potentially toxic species, such as blue-green algae, diatoms, cnidarians, ctenophores, Nassarius spp., and pufferfish. However, some edible and toxic species may look similar, and the correct identification of marine species is thus a major issue. The failure of traditional classification methods in certain species has promoted the use of DNA barcoding, which uses short, standard DNA fragments to assist with species ident

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Arian, Perkasa, I. Made Artika, and Syamsul Falah. "Amplification and Analysis of Cytocrome Oxidase I of Polypedates leucomystax from Bogor Agricultural University Area." Current Biochemistry 3, no. 1 (2017): 13–19. http://dx.doi.org/10.29244/cb.3.1.13-19.

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DNA barcoding has become a useful tool for identifying and confirming of species within a known taxonomic framework. A large-scale effort is underway to barcode all amphibian species using the universally sequenced DNA region, a partial fragment of mitochondrial cytochrome oxidase subunit I (COI). This study was aimed to use DNA barcoding technique to identify and confirm species of Polypedates leucomystax and to analyze their phylogenetic relationship. Samples of Polypedates leucomystax were collected from Campus Area of Bogor Agricultural University. The cytochrome oxidase I gene of 600-700

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Shetty, Ashwit S., and Hitesh U. Shingadia. "Applications of DNA Barcoding in Fisheries: A Review." UTTAR PRADESH JOURNAL OF ZOOLOGY 44, no. 23 (2023): 351–71. http://dx.doi.org/10.56557/upjoz/2023/v44i233796.

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The review article focuses on the widespread application of DNA barcoding in the fishery background. With the advancement in science and technology, there is a dire necessity for upgradation in approaches for taxonomy, wildlife conservation, and health management in the fishery industry. To reduce invasiveness, illegal fishing, and health complications in fish in both marine as well aquaculture ecosystems different required mitigative measures need to be enforced. DNA barcoding is one important tool in biological science. It involves the sequencing of a small DNA segment called a ‘barcode’ of

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Liu, Qin, Fei Zhu, Guanghui Zhong, et al. "COI-based barcoding of Chinese vipers (Reptilia: Squamata: Viperidae)." Amphibia-Reptilia 36, no. 4 (2015): 361–72. http://dx.doi.org/10.1163/15685381-00003012.

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DNA barcoding seeks to assemble a standardized reference library for rapid and unambiguous identification of species, and can be used to screen for potentially cryptic species. The 5′ region of cytochrome oxidase subunit I (COI), which is a mitochondrial DNA (mtDNA) gene fragment, has been proposed as a universal marker for this purpose among animals. However, DNA barcoding of reptiles is still supported only by few datasets compared with other groups. We investigated the utilization of COI to discriminate 34 putative species of vipers, representing almost 92% of the recorded species in China.

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Balasubramaniyan, Monisha, and Yoganandan Veeran. "Benthic Foraminifera and Diatom Relationship: Insights from RbcL Gene Sequences in Palk Bay." Current World Environment 19, no. 3 (2025): 1314–26. https://doi.org/10.12944/cwe.19.3.22.

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Understanding the symbiotic relationships between benthic foraminifera and diatoms is crucial for ecological studies, particularly in environmental changes. In this study, DNA barcoding, targeting the rbcL gene, was applied to identify diatoms and evaluate their diversity within the foraminiferal shells in Thondi, Palk Bay. Foraminifera were isolated from the sediment samples collected using grab samplers at six different locations. The separated benthic foraminifera were then used for DNA barcoding. The obtained DNA sequences were aligned and analyzed comprehensively using Geneious Pro v5.1.

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Hidayati, R. Misrianti, and A. Ali. "Phylogenetic tree of Kuantan cattle by DNA barcoding." Jurnal Ilmu Ternak dan Veteriner 21, no. 1 (2016): 41. http://dx.doi.org/10.14334/jitv.v21i1.1351.

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<p>Kuantan cattle is one of local beef cattle breed of Riau Province which its origin was unknown. Kuantan cattle are commonly found in Indragiri Hulu and Kuantan Singingi Regency. Based on phenotype characterizations, kuantan cattles are similar with pesisir cattle (West Sumatera beef cattle). Historically, kuantan cattle were pesisir cattle brought by “minang” immigrants (Immigrant from West Sumatera) to this region. The purpose of this study was to analyze the origin of the kuantan cattle through genetic diversity analysis using DNA barcode. DNA barcode used was Cytochrome oxidase sub

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Fattah, Yusuf R., Vanda S. Kamu, Max R. J. Runtuwene, and Lidya I. Momuat. "Identifikasi Barcode Tumbuhan Gedi Merah (Abelmoschus manihot L. medik) dan Gedi Hijau (Abelmoschus moschatus) Berdasarkan Gen matK." Jurnal MIPA 3, no. 2 (2014): 120. http://dx.doi.org/10.35799/jm.3.2.2014.5863.

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Gedi (Abelmoschus L.) merupakan tumbuhan tropis. Tumbuhan ini memilki efek farmakologis. Masyarakat Minahasa mengkonsumsi daun gedi yang direbus tanpa diberi bumbu sebagai obat tradisional untuk menurunkan kadar kolesterol, antihipertensi dan antidiabetes. Suatu metode baru untuk mengidentifikasi dan menganalisis keanekaragaman genetika spesies telah dikembangkan dengan menggunakan potongan gen standar yang dikenal dengan teknik DNA barcoding. Salah satu gen yang terdapat pada tumbuhan yaitu gen matK telah digunakan sebagai gen standar untuk barcoding. Pada penelitian ini telah dilakukan isola

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Kim, Hajin, Sang Eon Shin, Kwang Soo Ko, Seong Hwan Park, and Kexuan Tang. "The Application of Mitochondrial COI Gene-Based Molecular Identification of Forensically Important Scuttle Flies (Diptera: Phoridae) in Korea." BioMed Research International 2020 (September 28, 2020): 1–6. http://dx.doi.org/10.1155/2020/6235848.

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Phoridae are a family of necrophagous flies commonly found in indoor death scene. They account for approximately 19.7% of the entomofauna in human cadavers in Korea. Additionally, this taxon is an indicator of indoor hygiene, and these flies appear in environments where access by other necrophagous insects is difficult, such as enclosed rooms. Thus, they are likely to be used as forensic evidence. Despite their importance in forensic investigations and environmental hygiene, detailed studies on the taxonomy and molecular barcoding for this family are scarce, including in Korea. Because accurat

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Zhou, Tingting, Wei Jiang, Hongzhu Wang, and Yongde Cui. "DNA barcoding of Naididae (Annelida, Oligochaeta), based on cytochrome C oxidase gene and ITS2 region in China." Biodiversity Data Journal 9 (December 14, 2021): e73556. https://doi.org/10.3897/BDJ.9.e73556.

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Exploring the effectiveness of DNA barcoding in species identification is a prerequisite for biodiversity conservation and environmental monitoring. Aquatic oligochaetes could serve as excellent indicators in aquatic monitoring programmes. However, few studies have examined the effectiveness of DNA barcoding in these specific organisms. The mitochondrial cytochrome C oxidase (COI) gene of 83 specimens belonging to 40 species of 18 genera were sequenced in this study. The results showed that there was a barcode gap between species of Naididae and the intraspecific genetic distances of each spec

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Javaheri Tehrani, Sahar, Elham Rezazadeh, Niloofar Alaei Kakhki, et al. "DNA barcoding of passerine birds in Iran." ZooKeys 1236 (April 24, 2025): 19–39. https://doi.org/10.3897/zookeys.1236.143336.

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Exploring genetic diversity is essential for precise species delimitation, especially within taxonomically complex groups like passerine birds. Traditional morphological methods often fail to resolve species boundaries; however, DNA barcoding, particularly through the mitochondrial cytochrome c oxidase subunit I (COI) gene, provides a powerful complementary method for accurate species identification. This study establishes a comprehensive DNA barcode library for Iranian passerine birds, analyzing 546 COI sequences from 94 species across 23 families and 53 genera. There is a pronounced barcode

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Szyp-Borowska, Iwona, and Katarzyna Sikora. "DNA barcoding: A practical tool for the taxonomy and species identification of entomofauna." Forest Research Papers 80, no. 3 (2019): 227–32. http://dx.doi.org/10.2478/frp-2019-0021.

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AbstractDNA barcoding is an innovative system designed to provide rapid, accurate, and automatable species identification by using short, standardized gene regions as internal species codes. The mitochondrial cytochrome C oxidase I (COI) gene was proposed by Paul Hebert as an official marker for animals, because of its small intraspecific but large interspecific variation. Since the launch of the project Barcode of Life, this simple technique has caught the interest of taxonomists, ecologists and plant-quarantine officers charged with the control of pests and invasive species.The great diversi

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