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Journal articles on the topic 'Benzyl aminopurine'

Author: Grafiati

Published: 6 June 2025

Last updated: 25 July 2025

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1

A, KALAMANI. "INITIATION OF CALLUS AND PLANT REGENERATION IN COTTON (Gossypium hirsutum L.)." Madras Agricultural Journal 81, November (1994): 579–80. http://dx.doi.org/10.29321/maj.10.a01585.

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The hypocotyl explants of MDU 9 cultured on Murashige and Skoog medium supplemented with kinetin (1.0 mg/lit.)+ IAA (1.5 mg/lit.) formed callus in 20 days. The calli on sub culture to media containing benzyl amino purine (2.0 mg/lit.) developed shoots. Multiple shoots were formed from each callus mass. These on further sub culture with reduced level of benzyl aminopurine developed well. Root initiation and growth was promoted by lowering the sucrose concentration and supplementing the culture media with NAA (3.0 mg/lit.).

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2

Nada, Shadia, Siva Chennareddy, Stephen Goldman, et al. "Direct Shoot Bud Differentiation and Plantlet Regeneration from Leaf and Petiole Explants of Begonia tuberhybrida." HortScience 46, no. 5 (2011): 759–64. http://dx.doi.org/10.21273/hortsci.46.5.759.

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We report here an efficient and high-frequency protocol for direct shoot bud differentiation from mature leaves and petioles of greenhouse-grown Begonia tuberhybrida plants. Shoot buds were induced directly on the adaxial surface of leaf tissues from not only at the cut ends, but also across the entire surface of both leaf and petiole segments. The highest frequency of shoot bud formation was 90%, and the maximum number of shoots (132) per leaf explant was achieved with modified Murashige and Skoog (MS) (Murashige and Skoog, 1962) media supplemented with 1.0 mg·L−1 α-naphthalene acetic acid (N

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Nur Khozin, Mohammad, and Muhammad Dima Say Mona. "Induction and Regeneration of Tobacco Callus using Benzyl Aminopurine (BAP) and Furfuryl Aminopurine (Kinetin) With Thin Cell Layer (TCL) Technique." IOP Conference Series: Earth and Environmental Science 1497, no. 1 (2025): 012017. https://doi.org/10.1088/1755-1315/1497/1/012017.

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Abstract Tobacco was a high-value crop that required effective and efficient cultivation methods to produce quality seedlings. This study aimed to enhance the efficiency of tobacco cultivation through tissue culture using the Thin Cell Layer (TCL) method. The study took place at the Tissue Culture Laboratory, Faculty of Agriculture, University of Jember, between January and March 2024. Leaf bud explants from the Broadleaf One-Sucker tobacco variety were cultivated on Murashige & Skoog (MS) medium supplemented with different concentrations of BAP (0, 2, 3, 4 ppm) and Kinetin (0, 3, 4 ppm).

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Bonello, Mariella, Uroš Gašić, Živoslav Tešić, and Everaldo Attard. "Production of Stilbenes in Callus Cultures of the Maltese Indigenous Grapevine Variety, Ġellewża." Molecules 24, no. 11 (2019): 2112. http://dx.doi.org/10.3390/molecules24112112.

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The production of secondary metabolites in tissue culture has been considered as an alternative to the cultivation and harvesting of crops intended for this purpose. The present study was aimed at the growth of callus and production of polyphenolic compound of callus derived from a Maltese indigenous grapevine variety, Ġellewża. Callus was inoculated onto plant growth regulators-enriched Murashige Skoog media (MSm) to determine whether polyphenols are produced in vitro as well as to determine the best combination of plant growth regulators needed for the production of these metabolites. From r

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Rustikawati, Rustikawati, Catur Herison, Entang Inoriah, and Vera Dwisari. "Effect of BAP (6-Benzyl Aminopurine) on In Vitro Shoot Growth of Curcumas." AGRITROPICA : Journal of Agricultural Sciences 4, no. 1 (2021): 82–92. http://dx.doi.org/10.31186/j.agritropica.4.1.82-92.

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Curcuma sp has been widely investigated for its anti-cancer properties. Conventionally, vegetative propagation needs a long time to produce a large number of planting materials, so that it is necessary to find an alternative approach through in vitro propagation. The effect of BAP on the in vitro shoot formation of ‘temu putih’ and ‘temu putih’ has been investigated in this study. The experiment was a 4x2 factorial with 5 replications arranged in a completely randomized design. The first factor was the concentration of BAP i.e. 0, 1.5, 3, and 4.5 ppm. The second factor was the curcuma species

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6

Markovic, Marija, Mihailo Grbic, and Ana Sindelic. "Possibility of micropropagation of dianthus giganteiformis subsp. Kladovanus (Degen) SÓO by the method of proliferation of lateral shoots." Bulletin of the Faculty of Forestry, no. 94 (2006): 171–80. http://dx.doi.org/10.2298/gsf0694171m.

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The micropropagation of kladovanus carnation (Dianthus giganteiformis subsp kladovanus) was tested by the lateral shoot proliferation method. Micropropagation was performed on the medium with 1/2 MS mineral solution supplemented with 3% sucrose, 0.8% agar, 50 mg?L-1 mio-inositol, 0.025 mg?L-1 thiamin, 0.125 mg?L-1 nicotinic acid and 0.5 mg?L-1 glycine. The development of lateral shoots was induced on 6-benzyl-aminopurine (BAP) and ?-naphthaleneacetic acid (NAA). The best results were achieved on the medium with 1 mg?L-1 BAP and 0.1 mg?L-1 NAA. Rooting was successful on the medium without hormo

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Markovic, Marija, Mihailo Grbic, Dragana Skocajic, and Danijela Djunisijevic-Bojovic. "Effect of phytohormone balance on shoot multiplication and rooting of the species Dianthus serotinus Waldst. and Kit." Bulletin of the Faculty of Forestry, no. 95 (2007): 83–94. http://dx.doi.org/10.2298/gsf0795083m.

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The effect of different phytohormone concentrations on shoot multiplication and shoot rooting was researched on the species Dianthus serotinus. All nutritive media contained MS mineral solution, 3% sucrose, 0.8% agar, 100 mg?L-1 mioinositol, 0.05 mg?L-1 thiamin, 0.25 mg?L-1 nicotinic acid and 1 mg?L-1 glycine. For lateral shoot proliferation, the basic medium was supplemented with BAP (6-benzyl-aminopurine) in concentrations 0.1-2 mg?L-1 and NAA (?-naphthalene-acetic acid) in concentrations 0.1-1 mg?L-1. The best results were achieved on the medium with 0.5 mg?L-1 BAP and NAA. Rooting was most

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8

Afzal Hossain, Md. "Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato." American Journal of Agriculture and Forestry 3, no. 2 (2015): 25. http://dx.doi.org/10.11648/j.ajaf.20150302.13.

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9

Pandey, Ved Prakash, Jose Kudakasseril, Elizabeth Cherian, and George Patani. "COMPARISON OF TWO METHODS FOR IN VITRO PROPAGATION OF RAUWOLFIA SERPENTINA FROM NODAL EXPLANTS." INDIAN DRUGS 44, no. 07 (2007): 514–19. http://dx.doi.org/10.53879/id.44.07.p0514.

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Two different methods of in vitro multiplication of Rauwolfia serpentina from nodal explants were compared viz. multiplication via callus morphogenesis and that via shoot proliferation from axillary buds. The second method was found to be far better. The optimum shoot proliferation occurred on Murashige and Skoog (MS) medium supplemented with 1 mg/L naphthalene acetic acid (NAA) and 2 mg/L of benzyl aminopurine (BAP). The best rooting of shoots occurred on MS medium containing 4% sucrose and 1 mg/L of NAA. Solid and liquid MS media were found to be similar in supporting shoot proliferation. Th

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10

Pandey, Ved Prakash, Jose Kudakasseril, Elizabeth Cherian, and George Patani. "Comparison of two methods for in vitro propagation of Rauwolfia serpentina from nodal explants." Indian Drugs 44, no. 7 (2007): 514–19. https://doi.org/10.5281/zenodo.3597219.

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Two different methods of in vitro multiplication of Rauwolfia serpentina from nodal explants were compared viz. multiplication via callus morphogenesis and that via shoot proliferation from axillary buds. The second method was found to be far better. The optimum shoot proliferation occurred on Murashige and Skoog (MS) medium supplemented with 1 mg/L naphthalene acetic acid (NAA) and 2 mg/L of benzyl aminopurine (BAP). The best rooting of shoots occurred on MS medium containing 4% sucrose and 1 mg/L of NAA. Solid and liquid MS media were found to be similar in supporting shoot proliferation. Th

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Singh, BM, C. Wawrosch, SD Joshi, and B. Kopp. "Micropropagation of Bauhinia variegata L. from Tissue Culture." Nepal Journal of Science and Technology 13, no. 1 (2013): 39–41. http://dx.doi.org/10.3126/njst.v13i1.7397.

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Bauhinia variegate L. is a multipurpose tree and its micropropagation holds great promise in agroforestry. The sterilized seeds were first inoculated on Murashige and Skoog (MS) medium. Nodal cuttings from these seedlings grown in vitro were used as explants for micropropagation. Nodal cutting inoculated on the medium with various concentrations of BAP (benzyl-aminopurine) and NAA (£- naphthalene acetic acid) and separately generated varied results. Best propagation was recorded on MS medium supplemented with 1.0 ?M BAP with 0.05 ?M NAA. Propagated plants were successfully acclimatized and roo

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Yang, Chuan Gui, Bao Gao, and Jing Ran Liu. "Calcium Ion and Protocorm Differentiation for Cymbidium Hybrid Cymbidium Lucky Gloria × Cymbidium Lovely Moon 'Crescent'." Applied Mechanics and Materials 195-196 (August 2012): 475–79. http://dx.doi.org/10.4028/www.scientific.net/amm.195-196.475.

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The low protocorm rate of the hybrid Cymbidium Lucky Gloria × Cymbidium Lovely Moon Crescent (LLC) has long been a detriment to its propagation. In this paper, we describe the thorough research about the effects of calcium ion concentration on the protocorm differentiation for Cymbidium hybrid LLC in vitro through 4 levels of Ca2+ concentration in MS medium based on the trial test including medium experiment with different calcium concentrations and the use of calcium inhibitor La3+. Our studies revealed that Ca2+ played a key role in protocorm differentiation of LLC, which was against the gen

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13

Mendel, Peter. "EVALUATION OF SELECTED CHARACTERISTICS IN INDUSTRIAL HEMP AFTER PHYTOHORMONAL TREATMENT." Pakistan Journal of Agricultural Sciences 57, no. 01 (2020): 1–7. http://dx.doi.org/10.21162/pakjas/20.7586.

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Plant growth and development is significantly influenced by phytohormones – endogenous molecules present naturally in plants. The best known plant hormones are auxins and cytokinins. This study examined the possible effect of externally applied plant hormone analogues (growth regulators): 1-naphthyl acetic acid (NAA) and 6-benzyl aminopurine (BAP) on industrial fibre hemp (Cannabis sativa L., variety Bialobrzeskie). Plants were treated with three different concentrations of NAA (5, 10 and 20 mg/l) and three different concentrations of BAP (10, 25 and 50 mg/l). Morphological and physiological c

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14

Wang, Xiao-juan. "Effects of di-butyl phthalate on in vitro morphogenesis of Arabidopsis thaliana." Functional Plant Biology 30, no. 8 (2003): 909. http://dx.doi.org/10.1071/fp03005.

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Di-butyl phthalate (DBP) is one of the common environmental contaminants found both in terrestrial and aquatic ecosystems. However, little is known about its effects on plant morphogenesis. We report here a study examining the effects of DBP on morphogenesis of Arabidopsis thaliana. When stems and laminae of Arabidopsis were cultured on Murashige and Skoog medium supplemented with 6-benzyl aminopurine (6-BA, 2 mg L–1) and naphthalene acetic acid (NAA, 0.2 mg L–1), both produced callus and some shoots. However, the presence of 1�mg�L–1 DBP in the above medium inhibited formation of callus by st

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15

Pal, Ram, M. Dayamma, and V. Nagrare. "Effects of BAP (6-Benzyl aminopurine) on bud break and plantlet regeneration from the back bulbs of Calanthe yucksomnensis S.Z. Lucksom (Orchidaceae)." Indian Journal of Forestry 32, no. 1 (2009): 65–68. http://dx.doi.org/10.54207/bsmps1000-2009-47at4l.

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The effect of BAP (6-benzyl aminopurine) on bud break in the back bulbs of Calanthe yuksomnensis was studied. For this purpose, the back bulbs were soaked in aqueous solution of BAP (20-200 mg/l) for 24 hours. The back bulbs were packed in transparent polyethylene bags with moist sawdust and stored in dark at room temperature for 40 days. The BAP concentration 20-100 mg/l increased number of sprouted back bulbs whereas higher concentration decreased the number of sprouted bulbs. The highest number of sprouted bulbs was obtained from BAP 100 mg/l treatment and maximum shoot bud length was recor

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Massolo, Juan F., María L. Lemoine, Alicia R. Chaves, Analía Concellón, and Ariel R. Vicente. "Benzyl-aminopurine (BAP) treatments delay cell wall degradation and softening, improving quality maintenance of refrigerated summer squash." Postharvest Biology and Technology 93 (July 2014): 122–29. http://dx.doi.org/10.1016/j.postharvbio.2014.02.010.

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17

Ugare, Bharat, Kaushik Banerjee, S. D. Ramteke, et al. "Dissipation kinetics of forchlorfenuron, 6-benzyl aminopurine, gibberellic acid and ethephon residues in table grapes (Vitis vinifera)." Food Chemistry 141, no. 4 (2013): 4208–14. http://dx.doi.org/10.1016/j.foodchem.2013.06.111.

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18

Drew, Roderick A. "Improved Techniques for in Vitro Propagation and Germplasm Storage of Papaya." HortScience 27, no. 10 (1992): 1122–24. http://dx.doi.org/10.21273/hortsci.27.10.1122.

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A multiplication technique based on subculture of nodal sections from apically dominant shoots is described for papaya (Carica papaya L.). Best multiplication rates were obtained when single-node papaya sections were cultured on a modified De Fossard medium containing 0.5 μm of both BAP and NAA. Shoots that developed from axillary buds were dissected and cultured for 3 days on rooting medium containing 10 μm IBA and subsequently transferred to hormone-free Drew-Smith (DS) medium. Explant growth rate was significantly reduced by substitution of 1% fructose for 2% sucrose in the medium. However,

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Saini, Neha, Rashmi Verma, Rabia Basri Aziz, Ashmita Bhatt, Hem Chandra Pant, and Naveen Gaurav. "Effect of Growth Regulators on Direct Clonal Propagation and Analysis of Total Phenolic Content of Wild and Propagated Mucuna pruriens." Scientific Temper 13, no. 02 (2022): 354–59. http://dx.doi.org/10.58414/scientifictemper.2022.13.2.51.

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Mucuna pruriens is an important vegetable cover crop. Almost every part of the plant is thoughtto contain L-3,4 dihydroxyphenylalanine (L-Dopa). The present study describes a quick anddependable technique for high-constancy miniature spread. Helper nodal explants were refinedon Murashige and Skoog’s (MS) media boosted with different groupings of cytokinins from15-day-old refined explants. Throughout the primary culture on 35 to 45 mg/l 6-benzyl aminopurine (BAP) greatest no of shoots was delivered. The number of shoots expanded when theflawless shoots were exposed to re-refine on a similar hor

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Lavakumaran, Lavanya, and Thayamini H. Seran. "Effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of Aloe vera (L.) Burm. f." Chilean journal of agricultural research 74, no. 4 (2014): 497–501. http://dx.doi.org/10.4067/s0718-58392014000400018.

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Kumari, Meenakshi. "Modulation in growth and development of potato (Solanum tuberosum L.) microtubers by different concentration of 6-benzyl aminopurine." African Journal of Plant Science 14, no. 2 (2020): 102–7. http://dx.doi.org/10.5897/ajps2019.1897.

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Oulkar, Dasharath P., Kaushik Banerjee, and Sunil Kulkarni. "Multiresidue Analysis of Plant Growth Regulators in Grapes by Triple Quadrupole and Quadrupole–Time of Flight-Based Liquid Chromatography/Mass Spectrometry." Journal of AOAC INTERNATIONAL 94, no. 6 (2011): 1715–21. http://dx.doi.org/10.5740/jaoacint.sgeoulkar.

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Abstract A selective and sensitive LC-MS/MS method is presented for simultaneous determination of 12 plant growth regulators, viz., indol-3-acetic acid, indol-3-butyric acid, kinetin, zeatin, 6-benzyl aminopurine, gibberellic acid, abscisic acid, chlormequat chloride, forchlorfenuron, paclobutrazole, daminozide, and 2,4-dichlorophenoxy acetic acid, in bud sprouts and grape berries. The sample preparation method involved extraction of homogenized sample (5 g) with 40 mL methanol (80%), and final determination was by LC-MS/MS in the multiple reaction monitoring (MRM) mode with time segmentation

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Sa’adan, Hafizuddin, and Zarina Zainuddin. "CALLUS INDUCTION FROM LEAF EXPLANT OF FICUS DELTOIDEA VARKUNSTLERI." Science Heritage Journal 4, no. 1 (2020): 06–08. http://dx.doi.org/10.26480/gws.01.2020.06.08.

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Ficus deltoidea or commonly known as ‘mas cotek’ is a herbal plant indigenous to Southeast Asia including Malaysia and Indonesia. This plant is popular for its medicinal values such as improve blood circulation, regain energy and enhance fertility naturally for both men and women. The main objective of this study is to develop in vitro clonal propagation method for rapid production of F. deltoidea using different concentrations of benzyl aminopurine (BAP) through shoot induction and multiplication, rooting and subsequent establishment in soil following acclimatization. Surface sterilization of

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Waldecker, Mark A., and Donald L. Wyse. "Chemical and Physical Effects of the Accumulation of Glyphosate in Common Milkweed (Asclepias syriaca) Root Buds." Weed Science 33, no. 5 (1985): 605–11. http://dx.doi.org/10.1017/s0043174500082953.

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Distribution of14C-glyphosate [N-(phosphonomethyl)glycine] in chemically treated and physically manipulated common milkweed [Asclepias syriaca(L.) ♯ ASCSY] was studied in greenhouse and growth chamber experiments. Pretreatment with glyphosate at 0.6 and 1.1 kg ae/ha 3 days prior to14C-glyphosate application to leaves reduced the concentration of14C recovered from shoots and leaves above the14C-glyphosate-treated leaves but had no influence on the concentration of14C in proximal root buds. Partial removal of the shoot and root prior to the application of14C-glyphosate increased bud respiration

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Hartatie Hardjo, Popy, Ida Bagus Made Artadana, Sulistyo Emantoko Dwi Putra, and Asad Jan. "Keiki induction by cytokinin on Phalaenopsis spp." E3S Web of Conferences 374 (2023): 00026. http://dx.doi.org/10.1051/e3sconf/202337400026.

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A Keiki is the product of asexual propagation which naturally develop at the node of a flower stalk or cane. Phalaenopsis produce Keiki along the middle to end nodes on the flower stalk. Although keikis are an inefficient way to propagate orchids on a commercial scale, it is interesting to study the cytokinin hormone Benzyl Aminopurine (BAP) effect to stimulate keikis on a node along the flower stalk. Two nodes of each flower stalk were used in this experiment. Cytokinin hormone (0 mg L−1 , 2 000 mg L−1 , 4 000 mg L−1 , 6 000 mg L−1 BAP) was only applied once to the second, third, fourth, and

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Bajwa, Ali Ahsan, Muhammad Farooq, and Ahmad Nawaz. "Seed priming with sorghum extracts and benzyl aminopurine improves the tolerance against salt stress in wheat (Triticum aestivum L.)." Physiology and Molecular Biology of Plants 24, no. 2 (2018): 239–49. http://dx.doi.org/10.1007/s12298-018-0512-9.

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Pebriana, Fitriya, Sri Wiyatiningsih, and Pangesti Nugrahani. "PENGARUH KONSENTRASI 6-Benzyl Aminopurine (BAP) PADA MEDIA MS TERHADAP INDUKSI KULTUR JARINGAN CAKRAM BAWANG MERAH (Allium ascalonicum L.)." BERKALA ILMIAH AGROTEKNOLOGI - PLUMULA 6, no. 1 (2019): 1–13. http://dx.doi.org/10.33005/plumula.v6i1.1.

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Penelitian ini bertujuan untuk menentukan konsentrasi yang memberikan pengaruh terbaik untuk induksi kultur jaringan cakram bawang merah. Penelitian ini dilaksanakan pada bulan Agustus sampai November 2017 di Laboratorium Bioteknologi, Fakultas Pertanian, UPN “Veteran” Jawa Timur. Metode yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan 1 faktor dan diulang sebanyak 10 kali. Adapun perlakuan konsentrasi zat pengatur tumbuh terdiri dari 4 taraf perlakuan yaitu Z0 (Kontrol (MS)), Z1 (MS + BAP + 1,5 ppm), Z2 (MS + BAP + 2,5 ppm) dan Z3 (MS + BAP + 3,5 ppm). Parameter

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Saadatian, Bijan, Mohamad Kafi, Mohamad Banayan aval, and Jafar Nabati. "Effect of paclobutrazol and benzyl aminopurine application at two growth stages on yield and breaking dormancy of potato minitubers." Journal of Science and Technology of Greenhouse Culture 8, no. 3 (2017): 51–64. http://dx.doi.org/10.29252/ejgcst.8.3.51.

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Ohrt, J. M., S. P. Dutta, and T. Srikrishnan. "Hydrogen bonding of modified bases involving Watson-Crick sites: Crystal structure and conformation of Benzyl 6-aminopurine-7-carboxylate." Crystal Research and Technology 41, no. 7 (2006): 723–27. http://dx.doi.org/10.1002/crat.200510655.

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Maślanka, Małgorzata. "Effect of Light Spectrum, Sucrose Concentration, and 6-Benzyl-aminopurine on In Vitro Adventitious Bulb Formation in Tulipa tarda." Agronomy 15, no. 3 (2025): 642. https://doi.org/10.3390/agronomy15030642.

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This study presents the optimal light spectrum and medium composition for the in vitro induction of adventitious bulbs in T. tarda Stapf. Bulb scales, used as explants, were cultivated on 100% MS solid media with 3% or 6% sucrose and 0 or 0.5 µM BAP (6-benzyl-aminopurine) under a 16 h photoperiod and different fluorescent light treatments (white, red, blue). Darkness was used as a control. The cultures were maintained at 20 ± 2 °C for 12 weeks. The obtained results revealed that white light combined with 6% sucrose yielded the highest adventitious bulb formation, with an average of 12.1 ± 1.3

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Nigar, Qandeela, Hassan Mahmood ul, Rashid Mehmood Rana, Irfan Ali, Hesham S. Almoallim, and Mohammad Javed Ansari. "Optimization of embryo rescue technique for development of hybrid plants in stenospermic grapes." Emirates Journal of Food and Agriculture 36 (August 28, 2024): 1–11. https://doi.org/10.3897/ejfa.2024.126572.

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The development of seedless cultivars is a primary goal in grapevine breeding. Since grapes are stenospermic and tend to abort their embryos before development, traditional breeding methods often yield seedless cultivars at a low frequency. Therefore, embryo rescue has emerged as a promising approach for creating seedless grape cultivars. This study aimed to optimize the ideal sampling time and protocol for efficient embryo rescue in grapes. Ovules from immature berries collected at various days after pollination (20DAP, 30DAP, 40DAP, and 50DAP) were cultured on Nitsch and Nitsch (NN) medium w

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Kodad, Souhayla, Reda Melhaoui, Christophe Hano, et al. "Effect of Culture Media and Plant Growth Regulators on Shoot Proliferation and Rooting of Internode Explants from Moroccan Native Almond (Prunus dulcis Mill.) Genotypes." International Journal of Agronomy 2021 (April 29, 2021): 1–10. http://dx.doi.org/10.1155/2021/9931574.

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In this study, several methods have been used to facilitate shoot formation from nodal explants of local almond ecotypes known as “Beldi” grown in Eastern Morocco. Nodal segments of divers old local genotypes were cultured on various concentrations of auxin (indole-3-butyric acid (IBA)) and cytokinins (6-benzyl-aminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN)) added to two different media (Murashige and Skoog (MS) and Heller medium). The results showed that TDZ was more effective than the other tested hormones for in vitro proliferation of the “Beldi” ecotype. TDZ at the concentration o

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Pagani, Alberto, Danilo Carnelos, Jorge Molinari, Ernesto Giardina, and Adalberto Di Benedetto. "An exogenous cytokinin supply in the ornamental fern Asplenium nidus L. induces an unusual post-transplant biomass accumulation." Ornamental Horticulture 27, no. 2 (2021): 117–25. http://dx.doi.org/10.1590/2447-536x.v27i2.2259.

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Abstract Ferns are ornamental plants with a low relative growth rate and long production cycles, which are grown at small pot volumes to optimize the commercial space for sale. However, the root restriction effects under this plant management can limit biomass accumulation and frond area. Since an exogenous spray with cytokinin (6-benzyl aminopurine (BAP)) has been suggested as a tool to override the root restriction in plants grown in pots, this study aimed to evaluate the effect of different BAP doses (5, 50, 100 or 200 mg L-1) once (7 days after transplant), twice (7 and 30 days after trans

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KHOSRAVINIA, Sara, Seyed Mahdi ZIARATNIA, Abdolreza BAGHERI, Ghadir RAJABZADEH, and Seyed Hassan MARASHI. "Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.]." Notulae Scientia Biologicae 4, no. 4 (2012): 49–54. http://dx.doi.org/10.15835/nsb447963.

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The cell suspension culture and seed samples of Bunium persicum were extracted by supercritical fluid, hydrodistillation and solvent methods and analyzed by Gas Chromatography. In this study to compare the different methods of extractions, cuminaldehyde was targeted as one of the Black zira essential oil constitute. For callus induction the germinated seeds were cultured as explants on Murashige and Skoog medium supplemented with 2 mg/l 2,4-dichlorophenoxy acetic acid and 0.5 mg/l kinetin (treatment A) as well as 2 mg/l ?-naphthalene acetic acid and 0.5 mg/l 6-benzyl aminopurine (treatment B)

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Teshome, Shiferaw, and Tileye Feyissa. "Direct Organogenesis of Glinus lotoides L. ? Antihelmintic Herb Using Nodal Segments." Plant Tissue Culture and Biotechnology 26, no. 1 (2016): 131–41. http://dx.doi.org/10.3329/ptcb.v26i1.29774.

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Glinus lotoides L. is a valuable medicinal herb for the treatment of human and livestock ailments. However, it was listed under endangered plant species due to over utilization and lower seed viability. The purpose of this study has been to develop a micropropagation method from nodal segments of G. lotoides. MS containing various concentrations of Kn was used for culture induction. Best culture establishment (95%) and highest mean shoot number (2.45 ± 0.37) were achieved on a medium containing 1.5 mg/l Kn. Even though different concentrations of 6?benzyl aminopurine (BAP), Kn and ??naphthalen

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Zayova, Ely Georgieva, Maria Prokopova Geneva, Kamelia Dimitrova Miladinova-Georgieva, Marieta Georgieva Hristozkova, and Ira Valkova Stancheva. "Impact of Plant Growth Regulators on Greek oregano Micropropagation and Antioxidant Activity." Biosciences, Biotechnology Research Asia 16, no. 2 (2016): 297–305. http://dx.doi.org/10.13005/bbra/2746.

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This study highlights the development and achievements made for the micropropagation of Greek oregano (Origanum heracleoticum L. ) using stem tip explants. The shoots were cultured on Murashige and Skoog (MS) medium followed different concentrations of plant growth regulators (PGR) - 6-benzyl aminopurine, thidiazuron and zeatin at concentrations (0.5 or 1.0 mg L-1). The induction of multiple shoots from stem tip segments was the highest in MS medium supplemented with 1.0 mg L-1 zeatin. It was the most effective medium for shoot formation, which produced multiple shoots (2.7) with an average he

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Gonzales-Arteaga, Javier J., Juan Rodríguez-Layza, Ladislao C. Romero-Rivas, Adelmo Párraga-Quintanilla, and Julio A. Olivera-Soto. "Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties." Agroindustrial Science 13, no. 2 (2023): 93–102. http://dx.doi.org/10.17268/agroind.sci.2023.02.05.

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The objective was to determine the response of three strawberrry varieties (Fragaria x ananassa Duch.) to the aplication of two plant growth regulators: indol acetic acid (IAA) and 6-Benzyl aminopurine (BAP) added to Murashige & Skoog médium in strawberry micropropagation. The complete randomized block design was used and three strawberry varieties ‘Aromas’, ‘Albion’ and ‘Camarosa’ were considered as trials. MS medium for shoot regeneration in initial stage was supplemented with BAP 0.5 mgL-1 and IAA 0.6 mgL-1. Shoot proliferation MS medium was supplemented with BAP 0.5 mgL-1 in multiplica

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Sivakumar, P., M. Chitra, K. Sasikala, M. Selvamurugan, and V. Karunakaran. "An Overview of Pharmaceutical Applications and In vitro Micropropagation Techniques for Rare and Endangered Plant Species." Journal of Advances in Biology & Biotechnology 27, no. 9 (2024): 573–85. http://dx.doi.org/10.9734/jabb/2024/v27i91330.

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Many rare and endangered plant species possess valuable secondary metabolites with pharmacological applications. These bioactive compounds are often integral to traditional medicine systems, highlighting the cultural significance of these plants. The health benefits of many medicinal species are not fully validated by contemporary scientific research, and some may be facing extinction due to habitat loss, overharvesting or climate change. This situation highlights the urgent need for effective conservation strategies of the species and sustainable cultivation methods. Micropropagation is a val

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Babadjanova, Feruza I., Khurshida A. Ubaydullaeva, Akmal M. Asrorov, et al. "Improvement of callogenesis and somatic embryogenesis by selecting optimal hormonal balance in Sarnav and Desiree potato varieties." Agriculture (Pol'nohospodárstvo) 69, no. 1 (2023): 40–46. http://dx.doi.org/10.2478/agri-2023-0004.

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Abstract Callus formation and somatic embryogenesis in potato varieties are effective ways to obtain an entire plant from a single cell. The implementation of embryogenesis is widely used in improving plant materials and creating new biotechnological varieties. In our research, the optimum level of 1-naphthaleneacetic acid (NAA) and 6-benzyl aminopurine (BAP) was determined in the formation of callus tissue in the local Sarnav potato variety, and the Desiree variety taken as a control. Leaf and stem explants were used in the processes of callus tissue formation and somatic embryogenesis of bot

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Gandolfo, Esteban, Guido Hakim, Ernesto Giardina, and Adalberto Di Benedetto. "Involvement of cytokinins in biomass accumulation of Limonium sinuatum under root restriction during nursery." Ornamental Horticulture 28, no. 4 (2022): 453–58. http://dx.doi.org/10.1590/2447-536x.v28i4.2553.

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Abstract It has been suggested that the response of the specialty cut flower Limonium sinuatum to different abiotic stress situations related to the plug tray cell size during nursery could be associated with the synthesis and translocation of endogenous cytokinins produced in the root tips. To validate this hypothesis, the aim of this work was to evaluate the participation of cytokinins in the process of biomass accumulation in L. sinuatum plants through foliar spraying with a synthetic cytokinin (benzyl aminopurine, BAP) and an inhibitor of the synthesis of endogenous cytokinins (dopamine).

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Kodad, Souhayla, Reda Melhaoui, Mohamed Addi, et al. "Micropropagation of zygotic embryos from genetically diverse almond seedling orchards in eastern Morocco." E3S Web of Conferences 298 (2021): 03008. http://dx.doi.org/10.1051/e3sconf/202129803008.

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Numerous studies on perennial crops have shown that almond has a low tolerance to climate change, which led to detailed study for its adaptation by focusing on selecting heat-tolerant almond varieties. The long period of perennial agriculture creates particular challenges in a changing climate. The risk complicates the choice of a variety that the best variety for the current climate may be poorly suited for future climates. Hence the interest of having a large variability of tolerant genotypes. In the eastern Mediterranean region of Morocco, seedling almond trees are largely grown, which crea

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de Lojo, Juan María, Esteban Gandolfo, Verónica Feuring, Ernesto Benito Giardina, Carlos Luís Boschi, and Adalberto Di Benedetto. "Garden post-transplant effects of pre-transplant plug cell volume and growing medium quality (as abiotic stresses) in Impatiens walleriana." Ornamental Horticulture 27, no. 3 (2021): 320–33. http://dx.doi.org/10.1590/2447-536x.v27i3.2295.

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Abstract Although much is known about the production of bedding plants, including Impatiens walleriana, little has been documented on their post-production performance. Thus, the aim of this work was to understand how pre-transplant crop management related to root restrictions imposed by plug cell volume and substrate quality affects the post-production performance related to biomass accumulation. To this end, we tested four plug cell volumes, as well as four growing media with significantly different physical and chemical properties, during nursery and pot culture. We also evaluated the diffe

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Mekky, Hattem, Samah El Sohafy, Rasha Abu El-khair, and Amr El Hawiet. "TOTAL POLYPHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF SILYBUM MARIANUM CULTURES GROWN ON DIFFERENT GROWTH REGULATORS." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 4 (2017): 44. http://dx.doi.org/10.22159/ijpps.2017v9i4.16372.

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Objective: The present study was designed to study the effect of supplementing Silybum marianum cultures with different combinations and concentrations of growth regulators on the total phenolic content and the antioxidant activity of the produced cultures.Methods: The total polyphenolic content of 23 extracts of calli was calculated as µg of gallic acid/mg of dry weight extract (DWE) using Folin–Ciocalteu reagent. The antioxidant activity was calculated as trolox equivalent antioxidant capacity using 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) assay for extracts containing more than 10 µg gallic ac

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Nigar, Qandeela, Mahmood ul Hassan, Rashid Mehmood Rana, Irfan Ali, Hesham S. Almoallim, and Mohammad Javed Ansari. "Optimization of embryo rescue technique for development of hybrid plants in stenospermic grapes." Emirates Journal of Food and Agriculture 36 (August 28, 2024): 1–11. http://dx.doi.org/10.3897/ejfa.2024.126572.

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The development of seedless cultivars is a primary goal in grapevine breeding. Since grapes are stenospermic and tend to abort their embryos before development, traditional breeding methods often yield seedless cultivars at a low frequency. Therefore, embryo rescue has emerged as a promising approach for creating seedless grape cultivars. This study aimed to optimize the ideal sampling time and protocol for efficient embryo rescue in grapes. Ovules from immature berries collected at various days after pollination (20DAP, 30DAP, 40DAP, and 50DAP) were cultured on Nitsch and Nitsch (NN) medium w

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Peng, Meng-xuan, Ru-meng Chen, Qian Wei, et al. "Effects of Genotype, Light, and Plant Growth Regulators on Rhizome Browning, Proliferation, and Sprouting in Cymbidium." HortScience 58, no. 6 (2023): 671–76. http://dx.doi.org/10.21273/hortsci17119-23.

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Chinese cymbidiums are important flowering ornamental plants. Traditional propagation via seed or division cannot satisfy growers’ demand for commercialization of new cultivars, and in vitro propagation has a low micropropagation efficiency due to the browning of rhizomes. In this study, rhizomes of Cymbidium ‘14-16-13’ and ‘14-16-5’ were cultured on half-strength Murashige and Skoog (MS) medium supplemented with 6-benzyl aminopurine (BAP), NAA (α-napthaleneacetic acid), or BAP with NAA under either the dark or light. The degree of browning was read, and rhizome proliferation or sprouting (spr

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Ashok, Kumar, CP Singh, and Ram Sant. "Response of Plant Hormone bioassay to Paclobutrazol." International Journal of Plant Science and Horticulture 1, no. 3 (2019): 150–62. https://doi.org/10.36811/ijpsh.2019.110014.

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Quantitative estimation of a known or suspected biologically active substance (such as a hormone or drug) by measuring its effect on a living organism in standard conditions is known as bioassay. The interaction of gibberellic acid (GA3) and paclobutrazol (PBZ) in lettuce hypocotyl bioassay and 6-benzyl aminopurine (BAP) and paclobutrazol in radish cotyledon enlargement test that paclobutrazol counteracts gibberellin activity when applied exogenously. The radish cotyledon enlargement at low concentration without counteracting BAP action. The reduction of growth to be counteracting of gibberell

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Bojko, Monika, Magdalena Kędra, Agata Adamska, Zuzanna Jakubowska, Monika Tuleja, and Beata Myśliwa-Kurdziel. "Induction and Characteristics of Callus Cultures of the Medicinal Plant Tussilago farfara L." Plants 13, no. 21 (2024): 3080. http://dx.doi.org/10.3390/plants13213080.

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Tussilago farfara L. is a traditional medicinal plant valued for its potentially health-promoting metabolites. Its herbal raw material has been recognized and used since ancient times and continues to be widely used in traditional medicine. Introducing this plant species to in vitro cultivation is a challenging task, but once the protocol is developed, such cultures can provide an abundant and inexhaustible source of plant material. In this study, we report the successful induction and growth of vigorous T. farfara callus in vitro. Callus induction was achieved on MS solid media with the combi

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Chigozirim, Uzoma-Nwachukwu, Kpekot K.A., and Wilson V. "The Effect of Seed-Soaking in Cytokinin on the Vegetative Growth of White-Seed Melon (Cucumeropsis mannii Naudin)." GPH-International Journal of Biological & Medicine Science 07, no. 05 (2024): 31–38. https://doi.org/10.5281/zenodo.12665434.

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The study was conducted to investigate the effect of Cytokinin (6-Benzyl-aminopurine); BAP at two concentrations on the growth of <em>Cucumeropsis mannii </em>and was conducted at the Screen house of the Department of Plant Science and Biotechnology, Rivers State University, Port-Harcourt, Nigeria. <em>Cucumeropsis mannii </em>is a species of melon native to tropical West, Central and East Africa and cultivated widely in a number of countries including Nigeria, Benin republic and Cameroon primarily for its ‘leaves’ rich in nutrients and are of high medicinal value, which are consum

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Fauzan, Fadzlin Qistina, Arifullah Mohammed, Ahmed Mahmood Ibraheem, et al. "Plantlet regeneration of Cucumis melo L. Glamour cv. using different types of cytokinin and explants." Journal of Tropical Resources and Sustainable Science (JTRSS) 12, no. 1 (2024): 61–68. http://dx.doi.org/10.47253/jtrss.v12i1.1367.

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Plantlets or clones’ regeneration in Cucurbitaceae species can be performed in vitro by applying the tissue culture techniques. Hypocotyls, cotyledons, leaves, cotyledonary nodes and petioles can be used as the explants or starting materials to initiate the regeneration under laboratory condition. BAP (Benzyl aminopurine) and TDZ (Thidiazuron) were among the well-known cytokinin used in tissue culture as plant hormones to regulate the plant growth in vitro. The effectiveness of BAP and TDZ was investigated in this study to determine the effect of different types of cytokinin hormone on C. melo

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Jamilu, Danjuma Koko. "In vitro Propagation of Anther Culture of Datura (Datura stramonium. L) Using MS Media." International Journal of Pharmacy and Biological Sciences (IJPBS) 13, no. 3 (2023): 154–60. https://doi.org/10.5281/zenodo.10460721.

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AbstractDatura stramonium L. is a medicinal plant that makes tropane alkaloids and generates secondary metabolites during in-vitro culture. The study aimed at using standardizes explant (anther) surface sterilization to generate a virus free plant. A different culture of Datura stramonium was in-vitro propagated in MS basal medium with the addition of 30 g/L sucrose, 8 g/L agar, various mixtures of 2, 4, and Dichlophenoxyacetic acid (at three levels, 0, 1, and 2 mg/L), and kinetin (0, 0.25, 0.5, and 1 mg/L). The calli were then moved to regenerative media that contained BAP alone (at three lev

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