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Journal articles on the topic 'Beta-glucuronidase genes. Glucuronidase'

Author: Grafiati

Published: 4 June 2021

Last updated: 16 February 2022

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1

Swank, R. T., K. Pfister, D. Miller та V. Chapman. "The egasyn gene affects the processing of oligosaccharides of lysosomal β-glucuronidase in liver". Biochemical Journal 240, № 2 (1986): 445–54. http://dx.doi.org/10.1042/bj2400445.

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The accumulation of the relatively large amounts of beta-glucuronidase in microsomal fractions of normal mice depends on formation of complexes with the protein egasyn. Unexpectedly, it was found that the egasyn gene also affects the processing of beta-glucuronidase, which is segregated to lysosomes. In egasyn-positive mice lysosomal beta-glucuronidase from liver has a mean pI of 5.9 with a minor proportion at pI 5.4, whereas in egasyn-negative mice the proportion of the two lysosomal forms is reversed. Combined experiments measuring susceptibility to neuraminidase and to endoglycosidase H and

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2

Blankenship, J. E., and K. L. Kindle. "Expression of chimeric genes by the light-regulated cabII-1 promoter in Chlamydomonas reinhardtii: a cabII-1/nit1 gene functions as a dominant selectable marker in a nit1- nit2- strain." Molecular and Cellular Biology 12, no. 11 (1992): 5268–79. http://dx.doi.org/10.1128/mcb.12.11.5268.

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In Chlamydomonas reinhardtii, expression of the cabII-1 gene increases dramatically in response to light (cabII-1 encodes one of the light-harvesting chlorophyll a/b-binding proteins of photosystem II). We have used a region upstream of the cabII-1 gene in translational fusions to the bacterial uidA gene (encodes beta-glucuronidase) and transcriptional fusions to the Chlamydomonas nitrate reductase gene (nit1). Chlamydomonas transformants carrying intact copies of the chimeric uidA gene do not express beta-glucuronidase at the level of enzyme activity or mRNA accumulation. Methylation in the c

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Blankenship, J. E., and K. L. Kindle. "Expression of chimeric genes by the light-regulated cabII-1 promoter in Chlamydomonas reinhardtii: a cabII-1/nit1 gene functions as a dominant selectable marker in a nit1- nit2- strain." Molecular and Cellular Biology 12, no. 11 (1992): 5268–79. http://dx.doi.org/10.1128/mcb.12.11.5268-5279.1992.

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In Chlamydomonas reinhardtii, expression of the cabII-1 gene increases dramatically in response to light (cabII-1 encodes one of the light-harvesting chlorophyll a/b-binding proteins of photosystem II). We have used a region upstream of the cabII-1 gene in translational fusions to the bacterial uidA gene (encodes beta-glucuronidase) and transcriptional fusions to the Chlamydomonas nitrate reductase gene (nit1). Chlamydomonas transformants carrying intact copies of the chimeric uidA gene do not express beta-glucuronidase at the level of enzyme activity or mRNA accumulation. Methylation in the c

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4

Lund, S. D., D. Miller, V. Chapman, and R. E. Ganschow. "Androgen regulation of murine beta-glucuronidase expression: identification and characterization of a nonresponse variant." Genetics 119, no. 1 (1988): 151–56. http://dx.doi.org/10.1093/genetics/119.1.151.

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Abstract One of the major features of beta-glucuronidase (GUS) expression in inbred strains of the house mouse, Mus musculus, is the responsiveness of this enzyme to androgen stimulation in tubule cells of the kidney. Both GUS-specific and nonspecific mutations have been described which define genes that serve to control this response. During examination of the expression of GUS in the interbreeding subspecies, Mus hortulanus, a new GUS haplotype was uncovered that is characterized, in part, by a lack of GUS response to androgen stimulation in an apparently responsive kidney. Blot hybridizatio

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5

Sakamoto, W., N. R. Sturm, K. L. Kindle, and D. B. Stern. "petD mRNA maturation in Chlamydomonas reinhardtii chloroplasts: role of 5' endonucleolytic processing." Molecular and Cellular Biology 14, no. 9 (1994): 6180–86. http://dx.doi.org/10.1128/mcb.14.9.6180.

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Complex processing of primary transcripts occurs during the expression of higher-plant chloroplast genes. In Chlamydomonas reinhardtii, most chloroplast genes appear to possess their own promoters, rather than being transcribed as part of multicistronic operons. By generating specific deletion mutants, we show that petD, which encodes subunit IV of the cytochrome b6/f complex, has an RNA processing site that is required for accumulation of monocistronic petD mRNA in petD promoter deletion mutants; in such mutants, transcription of petD originates from the upstream petA promoter. The 5' ends of

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6

Sakamoto, W., N. R. Sturm, K. L. Kindle, and D. B. Stern. "petD mRNA maturation in Chlamydomonas reinhardtii chloroplasts: role of 5' endonucleolytic processing." Molecular and Cellular Biology 14, no. 9 (1994): 6180–86. http://dx.doi.org/10.1128/mcb.14.9.6180-6186.1994.

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Complex processing of primary transcripts occurs during the expression of higher-plant chloroplast genes. In Chlamydomonas reinhardtii, most chloroplast genes appear to possess their own promoters, rather than being transcribed as part of multicistronic operons. By generating specific deletion mutants, we show that petD, which encodes subunit IV of the cytochrome b6/f complex, has an RNA processing site that is required for accumulation of monocistronic petD mRNA in petD promoter deletion mutants; in such mutants, transcription of petD originates from the upstream petA promoter. The 5' ends of

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7

Dai, Wenhao, Yuanjie Su, Hongxia Wang, and Ceilo Castillo. "Agrobacterium-mediated Transformation of Buddleia Species." HortScience 44, no. 2 (2009): 526–28. http://dx.doi.org/10.21273/hortsci.44.2.526.

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Two Buddleia cultivars, B. davidii ‘Potters Purple’ and Buddleia ‘Lochinch’, were transformed using Agrobacterium tumefaciens strain EHA105 harboring the binary vector pBI121 carrying the neomycin phosphotransferase gene and β-glucuronidase gene (uidA). Transgenic plants were recovered from the Agrobacterium-infected leaf tissues through organogenesis in the selection medium (woody plant medium containing 250 mg·L−1 cefotaxime plus 500 mg·L−1 carbenicillin plus 40 mg·L−1 kanamycin). The rate of shoot regeneration from transformed leaf tissues increased from 5.7% to 32% through extending cocult

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8

JEOUNG, J. M., S. KRISHNAVENI, S. MUTHUKRISHNAN, H. N. TRICK, and G. H. LIANG. "Optimization of sorghum transformation parameters using genes for green fluorescent protein and beta-glucuronidase as visual markers." Hereditas 137, no. 1 (2002): 20–28. http://dx.doi.org/10.1034/j.1601-5223.2002.1370104.x.

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9

Verdonk, Julian C., and Michael L. Sullivan. "Artificial microRNA (amiRNA) induced gene silencing in alfalfa (Medicago sativa)." Botany 91, no. 2 (2013): 117–22. http://dx.doi.org/10.1139/cjb-2012-0166.

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Gene silencing is a powerful technique that allows the study of the function of specific genes by selectively reducing their transcription. Several different approaches can be used, however they all have in common the artificial generation of single stranded small ribonucleic acids (RNAs) that are utilized by the endogenous gene silencing machinery of the organism. Artificial microRNAs (amiRNA) can be used to very specifically target genes for silencing because only a short sequence of 21 nucleotides of the gene of interest is used. Gene silencing via amiRNA has been developed for Arabidopsis

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10

He, Xiaoling, Susan C. Miyasaka, Yi Zou, Maureen M. M. Fitch, and Yun J. Zhu. "Regeneration and Transformation of Taro (Colocasia esculenta) with a Rice Chitinase Gene Enhances Resistance to Sclerotium rolfsii." HortScience 45, no. 7 (2010): 1014–20. http://dx.doi.org/10.21273/hortsci.45.7.1014.

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Genetic engineering has the potential to improve disease resistance in taro [Colocasia esculenta (L.) Schott]. To develop a method to produce highly regenerable calluses of taro, more than 40 combinations of Murashige and Skoog (MS) media at full- or half-strength with varying concentrations of auxin [α-naphthaleneacetic acid (NAA) or 2, 4-dichlorophenoxyacetic acid (2, 4-D)], cytokinin [benzyladenine (BA) or kinetin], and taro extract were tested for callus initiation and plant regeneration. The best combination, MS medium with 2 mg·L−1 BA and 1 mg·L−1 NAA (M5 medium), was used to produce reg

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Handa, Takashi. "Transformation of Prairie Gentian (Eustoma grandiflorum) withAgrobacterium rhizogenes Harboring .BETA.-Glucuronidase (GUS) and Neomycin Phosphotransferase II (NPT II ) Genes." Engei Gakkai zasshi 64, no. 4 (1996): 913–18. http://dx.doi.org/10.2503/jjshs.64.913.

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12

Padilla, Isabel M. G., Agnieszka Golis, Adele Gentile, Carmine Damiano, and Ralph Scorza. "Evaluation of transformation in peach Prunus persica explants using green fluorescent protein (GFP) and beta-glucuronidase (GUS) reporter genes." Plant Cell, Tissue and Organ Culture 84, no. 3 (2006): 309–14. http://dx.doi.org/10.1007/s11240-005-9039-1.

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13

Toe, Evelyne, Adjéhi Dadié, Etienne Dako, Guillaume Loukou, Marcelin Koffi Dje, and Y. C. Blé. "Prevalence and potential virulence of Escherichia coli in ready-to-eat raw mixed vegetable salads in collective catering in Abidjan, Côte d’Ivoire." British Food Journal 120, no. 12 (2018): 2912–23. http://dx.doi.org/10.1108/bfj-09-2017-0484.

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Purpose Vegetable salads, despite their recognized health benefits, are an increasingly common cause of foodborne illness worldwide. The purpose of this paper is to determine the prevalence of E. coli with virulence genes in ready-to-eat raw mixed vegetable salads sold in collective catering in Abidjan. Design/methodology/approach A total of 436 strains of E. coli were isolated from 306 ready-to-eat raw mixed vegetables salads and then identified biochemically and molecularly based on the uidA gene responsible for beta-glucuronidase activity. The virulence genes were determined by polymerase c

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Bolle, C., S. Sopory, T. Lubberstedt, R. B. Klosgen, R. G. Herrmann, and R. Oelmuller. "The Role of Plastids in the Expression of Nuclear Genes for Thylakoid Proteins Studied with Chimeric [beta]-Glucuronidase Gene Fusions." Plant Physiology 105, no. 4 (1994): 1355–64. http://dx.doi.org/10.1104/pp.105.4.1355.

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15

Datta, S., R. H. Gomer, and R. A. Firtel. "Spatial and temporal regulation of a foreign gene by a prestalk-specific promoter in transformed Dictyostelium discoideum." Molecular and Cellular Biology 6, no. 3 (1986): 811–20. http://dx.doi.org/10.1128/mcb.6.3.811.

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We analyzed a developmentally regulated prestalk-specific gene from Dictyostelium discoideum encoding a cathepsin-like protease. A hybrid gene was constructed by fusing 2.5 kilobases of 5' flanking sequences and part of the coding region of the gene in-frame to the Escherichia coli beta-glucuronidase gene and was transformed into D. discoideum cells. In cells transformed with this vector, the gene fusion showed the same temporal regulation as the endogenous gene during multicellular development and, like endogenous prestalk genes, was highly inducible by cyclic AMP in in vitro cell cultures. M

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Bernd-Souza, R. B., M. F. Grossi de Sa, D. D. Ellis, and B. H. McCown. "A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants." Genetics and Molecular Biology 23, no. 2 (2000): 435–43. http://dx.doi.org/10.1590/s1415-47572000000200032.

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The effects of an animal RNase fused to the late cotton pollen-specific promoter G9 in a plant system were investigated. Expression of the chimeric genes G9-uidA and G9-RNase in tobacco plants showed that the 1.2-kb promoter fragment of the G9 gene was sufficient to maintain tissue and temporal specificity in a heterologous system. GUS (beta-glucuronidase) expression was detected only in pollen from anther stage 6 through anthesis, with maximal GUS activity in pollen from stage 10 anthers. Investigating the effects of the rat RNase on pollen viability at stage 10, we found that pollen viabilit

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17

Twell, D., J. Yamaguchi, and S. McCormick. "Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis." Development 109, no. 3 (1990): 705–13. http://dx.doi.org/10.1242/dev.109.3.705.

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To investigate the regulation of gene expression during male gametophyte development, we analyzed the promoter activity of two different genes (LAT52 and LAT59) from tomato, isolated on the basis of their anther-specific expression. In transgenic tomato, tobacco and Arabidopsis plants containing the LAT52 promoter region fused to the beta-glucuronidase (GUS) gene, GUS activity was restricted to pollen. Transgenic tomato, tobacco and Arabidopsis plants containing the LAT59 promoter region fused to GUS also showed very high levels of GUS activity in pollen. However, low levels of expression of t

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18

Datta, S., R. H. Gomer, and R. A. Firtel. "Spatial and temporal regulation of a foreign gene by a prestalk-specific promoter in transformed Dictyostelium discoideum." Molecular and Cellular Biology 6, no. 3 (1986): 811–20. http://dx.doi.org/10.1128/mcb.6.3.811-820.1986.

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We analyzed a developmentally regulated prestalk-specific gene from Dictyostelium discoideum encoding a cathepsin-like protease. A hybrid gene was constructed by fusing 2.5 kilobases of 5' flanking sequences and part of the coding region of the gene in-frame to the Escherichia coli beta-glucuronidase gene and was transformed into D. discoideum cells. In cells transformed with this vector, the gene fusion showed the same temporal regulation as the endogenous gene during multicellular development and, like endogenous prestalk genes, was highly inducible by cyclic AMP in in vitro cell cultures. M

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19

Wagoner, Wendy, J. Stamp, H. Matthews, J. Kellogg, and R. Bestwick. "473 PB 367 AGROBACTERIUM- MEDIATED TRANSFORMATION OF CAULIFLOWER FOR CONTROL OF FLOWER SENESCENCE." HortScience 29, no. 5 (1994): 499a—499. http://dx.doi.org/10.21273/hortsci.29.5.499a.

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Ethylene is a known causal factor in the decay and senescence of fruits and vegetables. The aim of the present study was to incorporate a gene for control of ethylene biosynthesis in order to prevent or delay the senescence of the cauliflower curds. We first developed a reproducible transformation system using marker genes for beta glucuronidase (GUS) and antibiotic resistance. Brassica oleraceae L. var. botrytis was transformed by inoculating hypocotyl explants with the Agrobacterium tumefaciens strains C58 or EHA101 containing plasmids pAG5110, pAG5420, or pAG5520. The plasmid pAG5110 contai

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Torres, Antonio Carlos, Adriana T. Ferreira, Carlos F. N. Widholzer, Eduardo Romano, and José A. Peters. "Expressão eficiente do gene reporter beta-glucuronidase nos tecidos vasculares de batata (Solanum tuberosum L.) utilizando de um promotor específico (BRA3) de Agrobacterium rhizogenes." Horticultura Brasileira 21, no. 2 (2003): 177–80. http://dx.doi.org/10.1590/s0102-05362003000200011.

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Promotores tecido-específico controlam a transcrição de genes em diferentes tecidos vegetais bem como em diferentes estádios de desenvolvimento da planta, levando à indução de distintos níveis de atividade transiente e/ou estável do gene. Tais promotores podem ser empregados para a expressão seletiva de genes de interesse. O promotor rol A de Agrobacterium rhizogenes, por exemplo, é floema-específico, sugerindo que possa ser empregado em estratégias de defesa de plantas que são infectadas por vírus com replicação restrita ao floema. A expressão do gene marcador da ß-glucuronidase (gus) dirigid

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HANCOCK, DALE D., DANIEL H. RICE, LEE ANN THOMAS, DAVID A. DARGATZ, and THOMAS E. BESSER. "Epidemiology of Escherichia coli O157 in Feedlot Cattle." Journal of Food Protection 60, no. 5 (1997): 462–65. http://dx.doi.org/10.4315/0362-028x-60.5.462.

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Fecal samples from cattle in 100 feedlots in 13 states were bacteriologically cultured for Escherichia coli O157 that did not ferment sorbitol, lacked beta-glucuronidase, and possessed genes coding for Shiga-like toxin. In each feedlot 30 fresh fecal-pat samples were collected from each of four pens: with the cattle shortest on feed, with cattle longest on feed, and with cattle in two randomly selected pens. E. coli O157 was isolated from 210 (1.8%) of 11,881 fecal samples. One or more samples were positive for E. coli O157 in 63 of the 100 feedlots tested. E. coli O157 was found at roughly eq

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Honma, T., and K. Goto. "The Arabidopsis floral homeotic gene PISTILLATA is regulated by discrete cis-elements responsive to induction and maintenance signals." Development 127, no. 10 (2000): 2021–30. http://dx.doi.org/10.1242/dev.127.10.2021.

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PISTILLATA is a B-class floral organ identity gene required for the normal development of petals and stamens in Arabidopsis. PISTILLATA expression is induced in the stage 3 flowers (early expression) and is maintained until anthesis (late expression). To explore in more detail the developmentally regulated gene expression of PISTILLATA, we have analyzed the PISTILLATA promoter using uidA (beta)-glucuronidase gene) fusion constructs (PI::GUS) in transgenic Arabidopsis. Promoter deletion analyses suggest that early PISTILLATA expression is mediated by the distal region and that late expression i

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Jermyn, K., D. Traynor, and J. Williams. "The initiation of basal disc formation in Dictyostelium discoideum is an early event in culmination." Development 122, no. 3 (1996): 753–60. http://dx.doi.org/10.1242/dev.122.3.753.

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We have analysed expression of the ecmA and ecmB genes of Dictyostelium by enzymatic double staining using beta-galactosidase and beta-glucuronidase reporter gene constructs. Cells expressing the ecmA gene first appear as scattered cells at the mound stage of development and we show that this is also true for cells expressing the ecmB gene. During tip formation the ecmA-expressing cells move to the apex of the mound, while the ecmB-expressing cells accumulate in the base. The ecmB-expressing cells constitute part of the basal disc if the culminant is formed in situ but are discarded if a migra

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Jamahari, Azreena, Wong Ling-Chie, Fan Xioalai, et al. "CHARACTERISATION OF HORDEUM VULGARE CELLULOSE SYNTHASE-LIKE F6 PROMOTER VIA TRANSGENE EXPRESSION IN RICE." Malaysian Journal of Science 40, no. 2 (2021): 61–86. http://dx.doi.org/10.22452/mjs.vol40no2.6.

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Beta-glucan in cereal crops is known as a functional food, which can reduce cardiovascular diseases by lowering blood cholesterol levels. However, beta-glucan content is relatively low in rice grains, despite being relatively abundant in barley and oat grains. Taking advantage of rice as the staple food for Asians, increasing beta-glucan content in rice for their consumption may help to reduce cardiovascular-related diseases among them. Previous attempts in increasing beta-glucan content in rice via transgene expression of beta-glucan synthase genes from barley into rice were unsuccessful due

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Hill, T. A., C. D. Day, S. C. Zondlo, A. G. Thackeray, and V. F. Irish. "Discrete spatial and temporal cis-acting elements regulate transcription of the Arabidopsis floral homeotic gene APETALA3." Development 125, no. 9 (1998): 1711–21. http://dx.doi.org/10.1242/dev.125.9.1711.

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The APETALA3 floral homeotic gene is required for petal and stamen development in Arabidopsis. APETALA3 transcripts are first detected in a meristematic region that will give rise to the petal and stamen primordia, and expression is maintained in this region during subsequent development of these organs. To dissect how the APETALA3 gene is expressed in this spatially and temporally restricted domain, various APETALA3 promoter fragments were fused to the uidA reporter gene encoding beta-glucuronidase and assayed for the resulting patterns of expression in transgenic Arabidopsis plants. Based on

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Nowak, W., M. Gawłowska, A. Jarmołowski, and J. Augustyniak. "Effect of nuclear matrix attachment regions on transgene expression in tobacco plants." Acta Biochimica Polonica 48, no. 3 (2001): 637–46. http://dx.doi.org/10.18388/abp.2001_3898.

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Matrix attachment regions (MARs) are thought to participate in the organization and segregation of independent chromosomal loop domains. Although there are several reports on the action of natural MARs in the context of heterologous genes in transgenic plants, in our study we tested a synthetic MAR (sMAR) with the special property of unpairing when under superhelical strain, for its effect on reporter gene expression in tobacco plants. The synthetic MAR was a multimer of a short sequence from the MAR 3' end of the immunoglobulin heavy chain (IgH) enhancer. This sMAR sequence was used to flank

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Yang, Jiangtao, Xujing Wang, Agula Hasi, and Zhixing Wang. "Structural and Functional Analysis of a Bidirectional Promoter from Gossypium hirsutum in Arabidopsis." International Journal of Molecular Sciences 19, no. 11 (2018): 3291. http://dx.doi.org/10.3390/ijms19113291.

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Stacked traits have become an important trend in the current development of genomically modified crops. The bidirectional promoter can not only prevent the co-suppression of multigene expression, but also increase the efficiency of the cultivation of transgenic plants with multigenes. In Gossypium hirsutum, Ghrack1 and Ghuhrf1 are head-to-head gene pairs located on chromosome D09. We cloned the 1429-bp intergenic region between the Ghrack1 and Ghuhrf1 genes from Gossypium hirsutum. The cloned DNA fragment GhZU had the characteristics of a bidirectional promoter, with 38.7% G+C content, three C

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Wang, Yan-Min, Chao Wang, Hui-Yan Guo, and Yu-Cheng Wang. "BplMYB46 from Betula platyphylla Can Form Homodimers and Heterodimers and Is Involved in Salt and Osmotic Stresses." International Journal of Molecular Sciences 20, no. 5 (2019): 1171. http://dx.doi.org/10.3390/ijms20051171.

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MYB proteins play important roles in the regulation of plant growth, development, and stress responses. Overexpression of BplMYB46 from Betula platyphylla improved plant salt and osmotic tolerances. In the present study, the interaction of eight avian myeloblastosis viral oncogene homolog (MYB) transcription factors with BplMYB46 was investigated using the yeast two-hybrid system, which showed that BplMYB46 could form homodimers and heterodimers with BplMYB6, BplMYB8, BplMYB11, BplMYB12, and BplMYB13. Relative beta-glucuronidase activity and chromatin immunoprecipitation assays showed that the

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A Otaiku, Ayodele, and AI Alhaji. "Fungi consortia in situ biodegradation of xenobiotic, military shooting range, Kachia, Kaduna, Nigeria." Journal of Applied Biotechnology & Bioengineering 7, no. 6 (2020): 246–74. http://dx.doi.org/10.15406/jabb.2020.07.00241.

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A major limitation of the white-rot fungus is its sensitivity during biodegradation of mixed matrix explosive pollutants and the scale of Kachia military shooting since 1967, Nigeria. The amplified 16S rRNA gene of each microbial isolate was processed for sequencing and characterization with Gene Bank database. Fungal species heavy metal reduction in increasing order of Aspergillus niger > Trametes versicolor > Rhizopus spp > Phanorochate chrysoporium > Penicillium spp were identified. The total explosive contents shows a significant difference for all locations in both dry and wet

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Feng, Zhi-Juan, Na Liu, Gu-Wen Zhang, Fu-Ge Niu, Sheng-Chun Xu, and Ya-Ming Gong. "Investigation of the AQP Family in Soybean and the Promoter Activity of TIP2;6 in Heat Stress and Hormone Responses." International Journal of Molecular Sciences 20, no. 2 (2019): 262. http://dx.doi.org/10.3390/ijms20020262.

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Aquaporins (AQPs) are one diverse family of membrane channel proteins that play crucial regulatory roles in plant stress physiology. However, the heat stress responsiveness of AQP genes in soybean remains poorly understood. In this study, 75 non-redundant AQP encoding genes were identified in soybean. Multiple sequence alignments showed that all GmAQP proteins possessed the conserved regions, which contained 6 trans-membrane domains (TM1 to TM6). Different GmAQP members consisted of distinct Asn-Pro-Ala (NPA) motifs, aromatic/arginine (ar/R) selectivity filters and Froger’s positions (FPs). Ph

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MOHRI, Takeshi, Tomohiro IGASAKI, Toru SATO, and Kenji SHINOHARA. "Expression of Genes for .BETA. - Glucuronidase and Luciferase in Three Species of Japanese Conifer (Pinus thunbergii, P. denslflora and Cryptomeria japonica) after Transfer of DNA by Microprojectile Bombardment." Plant Biotechnology 17, no. 1 (2000): 49–54. http://dx.doi.org/10.5511/plantbiotechnology.17.49.

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LeBlanc, Philippe M., Richard C. Hamelin, and Martin Filion. "Alteration of Soil Rhizosphere Communities following Genetic Transformation of White Spruce." Applied and Environmental Microbiology 73, no. 13 (2007): 4128–34. http://dx.doi.org/10.1128/aem.02590-06.

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ABSTRACT The application of plant genetic manipulations to agriculture and forestry with the aim of alleviating insect damage through Bacillus thuringiensis transformation could lead to a significant reduction in the release of pesticides into the environment. However, many groups have come forward with very valid and important questions related to potentially adverse effects, and it is crucial to assess and better understand the impact that this technology might have on ecosystems. In this study, we analyzed rhizosphere soil samples collected from the first B. thuringiensis-transformed trees

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Priya, A. M., S. K. Pandian, and R. Manikandan. "The effect of different antibiotics on the elimination of Agrobacterium and high frequency Agrobacterium-mediated transformation of indica rice (Oryza sativa L.)." Czech Journal of Genetics and Plant Breeding 48, No. 3 (2012): 120–30. http://dx.doi.org/10.17221/77/2011-cjgpb.

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We report here the suitable explant with high efficiency of transformation and the positive effects of timentin over other antibiotics like carbenicillin and cefotaxime on the elimination of Agrobacterium tumefaciens during the genetic transformation of popular indica rice (Oryza sativa L.). The tissues assayed were embryogenic calli, embryos with endosperm contamination, intact seeds, leaf blades, leaf bases and coleoptiles. The frequency of transient &beta;-glucuronidase (GUS) expression as revealed by histochemical assay was 90% for embryogenic calli, which was the highest among the exp

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Parin, U., S. Kirkan, SS Arslan, and HT Yuksel. "Molecular identification and antimicrobial resistence of Escherichia fergusonii and Escherichia coli from dairy cattle with diarrhoea." Veterinární Medicína 63, No. 3 (2018): 110–16. http://dx.doi.org/10.17221/156/2017-vetmed.

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The aim of this study was to determine the incidence of Escherichia fergusonii in dairy cattle with clinical signs of diarrhoea. The specimens were obtained from three different farms in Denizli province of Turkey, between August 2016 and December 2016. Rectal contents of 57 Holstein-friesian dairy cattle with diarrhoea were collected from farms located in the Aegean Region (Denizli province, Turkey). Rectal swabs were inoculated into enrichment, differential and selective culture media. A total of 49 (86%) Escherichia spp. were isolated by phenotypic identification from 57 rectal swab samples

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Steele, Marina, Kim Ziebell, Yongxiang Zhang, et al. "Identification of Escherichia coli O157:H7 Genomic Regions Conserved in Strains with a Genotype Associated with Human Infection." Applied and Environmental Microbiology 73, no. 1 (2006): 22–31. http://dx.doi.org/10.1128/aem.00982-06.

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ABSTRACT Beta-glucuronidase-negative, sorbitol-nonfermenting isolates of Shiga toxin-producing Escherichia coli O157 comprise part of a clone complex of related enterohemorrhagic E. coli isolates. High-resolution genotyping shows that the O157 populations have diverged into two different lineages that appear to have different ecologies. To identify genomic regions unique to the most common human-associated genotype, suppression subtractive hybridization was used to identify DNA sequences present in two clinical strains representing the human lineage I O157:H7 strains but absent from two bovine

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Kabirnataj, Sara, Ghorbanali Nematzadeh, Jafar Zolala, and Ahmad Farhad Talebi. "High-efficient transgenic hairy roots induction in chicory: re-dawn of a traditional herb." Acta agriculturae Slovenica 107, no. 2 (2016): 321. http://dx.doi.org/10.14720/aas.2016.107.2.06.

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<p>Plant roots can be manipulated by <em>Agrobacterium rhizogenes</em> to stimulate the production of heterologous proteins for pharmaceutical applications as green cell-factories. During the present study, four bacterial strains (A4, ATCC15834, ATCC11325 and A13) in combination with three co-cultivation media (MS, B5, LS) were examined to establish an efficient and reliable transformation system for chicory (<em>Cichorium intybus</em> L.) using <em>A. rhizogenes</em>. The maximum chicory hairy roots induction was achieved using A13 strain. The observa

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Maga, J. A., G. Widmer, and J. H. LeBowitz. "Leishmania RNA virus 1-mediated cap-independent translation." Molecular and Cellular Biology 15, no. 9 (1995): 4884–89. http://dx.doi.org/10.1128/mcb.15.9.4884.

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Recently, a group of related Leishmania RNA viruses (Leishmania RNA virus 1 [LRV1]) has been isolated from Leishmania guyanensis and L. brasiliensis. These viruses persist in the cytoplasm and contain double-stranded RNA genomes. Miniexon sequences are absent from the 5' end of the viral RNA, and the 5' end of the viral RNA lacks a cap structure, suggesting that LRV1 has evolved a cap-independent mechanism of translation. Cap-independent translation of picornavirus genomic RNA requires a cis element, within the 5' untranslated region (UTR), referred to as an internal ribosome entry site (IRES)

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Gu, Q., C. Ferrandiz, M. F. Yanofsky, and R. Martienssen. "The FRUITFULL MADS-box gene mediates cell differentiation during Arabidopsis fruit development." Development 125, no. 8 (1998): 1509–17. http://dx.doi.org/10.1242/dev.125.8.1509.

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Fruit morphogenesis is a process unique to flowering plants, and yet little is known about its developmental control. Following fertilization, fruits typically undergo a dramatic enlargement that is accompanied by differentiation of numerous distinct cell types. We have identified a mutation in Arabidopsis called fruitfull (ful-1), which abolishes elongation of the silique after fertilization. The ful-1 mutation is caused by the insertion of a DsE transposable enhancer trap element into the 5′ untranslated leader of the AGL8 MADS-box gene. beta-glucuronidase (GUS) reporter gene expression in t

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Topping, J. F., W. Wei, and K. Lindsey. "Functional tagging of regulatory elements in the plant genome." Development 112, no. 4 (1991): 1009–19. http://dx.doi.org/10.1242/dev.112.4.1009.

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In comparison with animals, relatively few plant genes have been identified that have been shown to be under organ-, tissue- or cell-type-specific regulation. In this paper, we describe how the beta-glucuronidase (GUS) reporter gene (gusA or uidA), fused to a weak promoter (a truncated (−90 bp) CaMV35S promoter), can be used to identify tissue-specific markers in transgenic tobacco plants. The rationale was that the expression of gusA would be determined primarily by position effect. Quantitative analysis revealed that, of 184 −90-gus transgenic plants, 73% exhibited gusA gene activation in le

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Lassoued, Mohamed Amin Ben, Nathalie Beaufils, Anderson Dieudonné Loundou, Christiane Arnaud, and Jean Gabert. "The Use of ABL and GUS as Control Genes for Normalizing Quantification of Fusion Transcripts by Real Time PCR: Our Experience of More Than 1000 Samples." Blood 114, no. 22 (2009): 4727. http://dx.doi.org/10.1182/blood.v114.22.4727.4727.

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Abstract Abstract 4727 Introduction Real-time quantitative PCR (RT-PCR) is a sensitive and accurate tool for monitoring leukemic patients by amplification of fusion gene (FG) transcripts. In order to correct variations in RNA quality and quantity and to calculate the sensitivity of each measurement, a control gene (CG) transcript should be amplified in parallel to the FG transcript. In our laboratory two CG are used: Abelson (ABL) and beta-glucuronidase (GUS). Clinical application for monitoring the treatment of chronic myelogenous leukemia (CML) patients with tyrosine kinase inhibitor makes t

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Conley, T. R., S. C. Park, H. B. Kwon, H. P. Peng, and M. C. Shih. "Characterization of cis-acting elements in light regulation of the nuclear gene encoding the A subunit of chloroplast isozymes of glyceraldehyde-3-phosphate dehydrogenase from Arabidopsis thaliana." Molecular and Cellular Biology 14, no. 4 (1994): 2525–33. http://dx.doi.org/10.1128/mcb.14.4.2525.

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We have characterized cis-acting elements involved in light regulation of the nuclear gene (GapA) encoding the A subunit of chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in Arabidopsis thaliana. Our results show that a 1.1-kb promoter fragment of the GapA gene is sufficient to confer light inducibility and organ specificity in transgenic Nicotiana tabacum (tobacco) plants, using the beta-glucuronidase gene of Escherichia coli as the reporter gene. Deletion analysis indicates that the -359 to -110 bp region of the GapA gene is necessary for light responsiveness. Within this regio

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Winder, A. J., A. Wittbjer, E. Rosengren, and H. Rorsman. "The mouse brown (b) locus protein has dopachrome tautomerase activity and is located in lysosomes in transfected fibroblasts." Journal of Cell Science 106, no. 1 (1993): 153–66. http://dx.doi.org/10.1242/jcs.106.1.153.

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Many genes mapping to pigmentation loci are involved in the regulation of melanin synthesis in the mouse. The brown (b) locus controls black/brown coat coloration, and its product has significant homology to the key melanogenic enzyme tyrosinase. This has led to suggestions that the b-protein is itself a melanogenic enzyme. In order to investigate its function, we have established lines of mouse fibroblasts stably expressing the b-protein by co-transfection of a b-protein expression vector and a plasmid conferring resistance to the antibiotic G418. The b-protein synthesised by these cells has

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Conley, T. R., S. C. Park, H. B. Kwon, H. P. Peng, and M. C. Shih. "Characterization of cis-acting elements in light regulation of the nuclear gene encoding the A subunit of chloroplast isozymes of glyceraldehyde-3-phosphate dehydrogenase from Arabidopsis thaliana." Molecular and Cellular Biology 14, no. 4 (1994): 2525–33. http://dx.doi.org/10.1128/mcb.14.4.2525-2533.1994.

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We have characterized cis-acting elements involved in light regulation of the nuclear gene (GapA) encoding the A subunit of chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in Arabidopsis thaliana. Our results show that a 1.1-kb promoter fragment of the GapA gene is sufficient to confer light inducibility and organ specificity in transgenic Nicotiana tabacum (tobacco) plants, using the beta-glucuronidase gene of Escherichia coli as the reporter gene. Deletion analysis indicates that the -359 to -110 bp region of the GapA gene is necessary for light responsiveness. Within this regio

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Khan, Khurum Hayat, Anna-Mary Young, Joaquin Mateo, et al. "Phase I clinical trial of a genetically modified and oncolytic vaccinia virus GL-ONC1 with green fluorescent protein imaging (NCT009794131)." Journal of Clinical Oncology 31, no. 15_suppl (2013): 3062. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.3062.

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3062 Background: GL-ONC is a genetically engineered virus attenuated by insertion of the ruc-gfp (Renilla luciferase and Aequorea green fluorescent protein fusion gene), beta-galactosidase (lacZ) and beta-glucuronidase (gusA) reporter genes into the FL14.5L, J2R (thymidine kinase) and A56R (hemagglutinin) loci, respectively. A phase I trial of intravenous (i.v) GL-ONC1 was pursued to evaluate safety, tolerability, tumour delivery, neutralising antibody development and antitumor activity. Methods: GL-ONC1 was administered at escalating doses (1x105, 1x106, 1x107, 1x108, 1x109, 3x109 plaque form

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Ross, David M., Dale B. Watkins, Timothy P. Hughes, and Susan Branford. "Reverse Transcription with Random Pentadecamer Primers Improves the Detection Limit of a Quantitative PCR Assay for BCR-ABL Transcripts in Chronic Myeloid Leukemia: Implications for Defining Sensitivity in Minimal Residual Disease." Clinical Chemistry 54, no. 9 (2008): 1568–71. http://dx.doi.org/10.1373/clinchem.2008.105916.

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Abstract Background: Real-time quantitative reverse transcription PCR (RQ-PCR) assay for BCR-ABL is used to monitor treatment response in chronic myeloid leukemia (CML). BCR-ABL transcript levels decline over several years of imatinib treatment, and increasing numbers of patients have BCR-ABL transcripts at or below the limit of detection. More sensitive PCR methods are required to assess whether these patients have a long-term continuing decline in residual disease. Methods: We used random pentadecamer (R15) primers for reverse transcription in RQ-PCR and compared the results with our establi

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46

Corral Jaime, Jesus, Anne Mary Young, Joaquin Mateo, et al. "Phase I clinical trial of a genetically modified and oncolytic vaccinia virus GL-ONC1 with green fluorescent protein imaging." Journal of Clinical Oncology 30, no. 15_suppl (2012): 2530. http://dx.doi.org/10.1200/jco.2012.30.15_suppl.2530.

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2530 Background: GL-ONC1 is a genetically engineered vaccinia virus attenuated by insertion of the RUC-GFP (Renilla luciferase and Aequorea green fluorescent protein fusion gene), beta-galactosidase (lacZ) and beta-glucuronidase (gusA) reporter genes into the F14.5L, J2R (thymidine kinase) and A56R (hemaglutinin) loci. A phase I clinical trial of iv GL-ONC1 was pursued to evaluate safety, tolerability, tumour delivery, neutralizing antibody development and anti-tumour activity. Methods: GL-ONC1 was administered to patients with advanced solid tumours at escalating doses (1×105, 1×106, 1×107, 1

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47

Vigneri, Paolo, Fabio Stagno, Stefania Stella, et al. "High BCR-ABL/GUSIS Levels at Diagnosis Are Associated with Unfavorable Responses to Standard Dose Imatinib." Blood 126, no. 23 (2015): 4049. http://dx.doi.org/10.1182/blood.v126.23.4049.4049.

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Abstract Background The approval of second-generation tyrosine kinase inhibitors (TKIs) for the first line treatment of Chronic Myeloid Leukemia (CML) has generated a need for early molecular parameters associated with inadequate responses to Imatinib Mesylate (IM). Objective We correlated quantitative determination of BCR-ABL transcripts at diagnosis with the outcome (defined according to the 2013 European Leukemia Net recommendations) of 272 newly diagnosed CML patients receiving IM 400 mg/die. Methods BCR-ABL transcripts were measured from peripheral blood samples drawn at diagnosis before

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Wącław, Joanna, Magdalena Zawada, Sylwia Czekalska, Bogdan Ochrem, and Tomasz Sacha. "Comparison of ABL1 and Gusb Reference Genes in the qRT-PCR Analysis of Halving Time and Early Molecular Response to TKI Therapy in Patients with Chronic Myeloid Leukemia." Blood 128, no. 22 (2016): 5424. http://dx.doi.org/10.1182/blood.v128.22.5424.5424.

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Abstract Objectives and background: Early molecular response (EMR) to tyrosine kinase inhibitors (TKI) therapy defined as BCR-ABL1 transcript level ≤ 10% at 3 months is a well-established predictive factor in CML patients. Recently, several study groups presented the concept of BCR-ABL1 decline velocity or "halving time" as a new predictive factor for optimal TKI treatment response. Different reference genes were used in the quantitative RT-PCR (qRT-PCR) analysis to calculate halving time and EMR rate, potentially influencing results of these analyses. The aim of the study was to compare ABL1

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Mueller, Martin C., Philipp Erben, Giuseppe Saglio, et al. "Harmonization of BCR-ABL mRNA Quantification Using an Uniform Control Plasmid in 36 International Laboratories." Blood 106, no. 11 (2005): 1991. http://dx.doi.org/10.1182/blood.v106.11.1991.1991.

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Abstract Quantitative determination of residual BCR-ABL transcript levels has been accepted as integral part of the management of CML patients. However, heterogeneity of molecular approaches results in a lack of comparability between different studies. Thus, there is an unmet need for harmonization of both procedures and expression of results. In a series of consensus meetings within the European LeukemiaNet a list of prerequisites to achieve an optimal sensitivity and standardization has been elaborated: use of at least 10ml peripheral blood processed within 36 hrs; bedside RNA stabilization

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Mizoguchi, Yoko, Sebastian Hesse, Monika Linder, et al. "Defects in Signal Recognition Particle (SRP) Components Reveal an Essential and Non-Redundant Role for Granule Biogenesis and Differentiation of Neutrophil Granulocytes." Blood 134, Supplement_1 (2019): 216. http://dx.doi.org/10.1182/blood-2019-130083.

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Neutrophil granulocyte play pivotal roles in inflammatory responses, immune defence, tissue remodeling, and cancer control. Studying rare patients with defects in differentiation and/or function of neutrophil granulocytes highlights genes and pathways orchestrating these important cellular functions. A previously not appreciated role of the signal recognition particle (SRP) has emerged when monoallelic mutations in SRP54 were associated with congenital neutropenia and pancreatic insufficiency. The eukaryotic SRP is composed of six distinct polypeptides (SRP9, SRP14, SRP19, SRP54, SRP68, SRP72)

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