Academic literature on the topic 'Bienertia sinuspersici'

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Journal articles on the topic "Bienertia sinuspersici"

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Kim, Beomsoo, Jingyu Kim, Hyun Park, and Joonho Park. "The complete chloroplast genome sequence of Bienertia sinuspersici." Mitochondrial DNA Part B 1, no. 1 (January 1, 2016): 388–89. http://dx.doi.org/10.1080/23802359.2016.1174083.

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Soundararajan, Prabhakaran, So Youn Won, Dong Suk Park, Yeon-Hee Lee, and Jung Sun Kim. "Comparative Analysis of the YABBY Gene Family of Bienertia sinuspersici, a Single-Cell C4 Plant." Plants 8, no. 12 (November 22, 2019): 536. http://dx.doi.org/10.3390/plants8120536.

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The emergence and expression of the YABBY gene family (YGF) coincided with the evolution of leaves in seed plants, and was integral to the early evidence of lamina followed by reproductive development. YGF contains six subclasses, i.e., CRC, INO, FIL, YAB2, YAB3, and YAB5. This study aims to extract the genome sequences of the YGF in Bienertia sinuspersici, an important model plant for single-cell C4 (SCC4), non-Kranz photosynthesis. A comparative genomic analysis was undertaken with Vitis vinefera, Arabidopsis thaliana, Brassica rapa, and Chenopodium quinoa. Six copies of YGF were present in B. sinuspersici and A. thaliana with a single copy of each YGF subgroup. V. vinefera possessed seven copies of YGF with duplicates in FIL and YAB2 subgroups, but no YAB3. B. rapa and C. quinoa after whole genome duplication contained additional copies of YGF. The gene structure and conserved motifs were analyzed among the YGF. In addition, the relative quantification of YGF was analyzed in the leaves, reproductive developmental stages such as the bud, and the pre-anthesis and anthesis stages in B. sinuspersici, A. thaliana, and B. rapa. CRC and INO possessed conserved floral-specific expression. Temporal and perpetual changes in the expression of YGF orthologs were observed in the leaves and reproductive developmental stages. The results of this study provide an overview of YGF evolution, copy number, and its differential expression in B. sinuspersici. Further studies are required to shed light on the roles of YABBY genes in the evolution of SCC4 plants and their distinct physiologies.
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Lung, Shiu-Cheung, Makoto Yanagisawa, and Simon DX Chuong. "Isolation of dimorphic chloroplasts from the single-cell C4 species Bienertia sinuspersici." Plant Methods 8, no. 1 (2012): 8. http://dx.doi.org/10.1186/1746-4811-8-8.

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Park, Joonho, Thomas W. Okita, and Gerald E. Edwards. "Expression profiling and proteomic analysis of isolated photosynthetic cells of the non-Kranz C4 species Bienertia sinuspersici." Functional Plant Biology 37, no. 1 (2010): 1. http://dx.doi.org/10.1071/fp09074.

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Bienertia sinuspersici Akhani represents one form of C4 photosynthesis that occurs without Kranz anatomy in family Chenopodiaceae. Analysis of transcript profiles and proteomics were made to gain information on this single-cell C4 photosynthetic mechanism. Chlorenchyma cells were isolated and purified from mature leaves. From these cells, a cDNA library was made from which sequences were obtained on 2385 clones using conventional methods. To obtain a protein profile, the multi dimensional protein identification technique was used, resulting in identification of 322 unique proteins in chlorenchyma cells. After analysing datasets from the EST library and proteomics, genes and proteins were classified into 23 and 17 categories according to types of biological processes, respectively. These include photosynthesis and photorespiration, other biosynthetic and metabolic processes, cell wall modification, defence response, DNA repair, electron transport, other cellular and developmental processes, protein folding, protein targeting, protein modification, proteolysis, redox and ion homeostasis, response to biotic and abiotic stresses, RNA modification, transcription, translation, transport and unknowns. Sequence and phylogenetic analyses were made of C4 cycle enzymes to characterise the relationship between homologues found in Bienertia with public gene sequences from other chenopods and representative C3 and C4 species from other families. Identified photosynthetic genes and proteins are discussed with respect to the proposed function of an NAD-ME type C4 cycle in this single-cell C4 system.
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Northmore, Jennifer Anne, Marie Leung, and Simon Dich Xung Chuong. "Effects of Media Composition and Auxins on Adventitious Rooting of Bienertia sinuspersici Cuttings." Advances in Bioscience and Biotechnology 06, no. 10 (2015): 629–36. http://dx.doi.org/10.4236/abb.2015.610066.

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Sevilleno, Samantha Serafin, Yoon Ha Ju, Jung Sun Kim, Franklin Hinosa Mancia, Eun Ju Byeon, Raisa Aone Cabahug, and Yoon-Jung Hwang. "Cytogenetic analysis of Bienertia sinuspersici Akhani as the first step in genome sequencing." Genes & Genomics 42, no. 3 (January 4, 2020): 337–45. http://dx.doi.org/10.1007/s13258-019-00908-5.

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Shwaish, Tarik, Faris JM Al-Imarah, and Fatimah A. Jasim. "Wound Healing Capacity, Antibacterial Activity, and GC-MS Analysis of Bienertia sinuspersici Leaves Extract." Journal of Physics: Conference Series 1294 (September 2019): 062056. http://dx.doi.org/10.1088/1742-6596/1294/6/062056.

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Northmore, Jennifer Anne, Victoria Zhou, and Simon D. X. Chuong. "Multiple shoot induction and plant regeneration of the single-cell C4 species Bienertia sinuspersici." Plant Cell, Tissue and Organ Culture (PCTOC) 108, no. 1 (August 21, 2011): 101–9. http://dx.doi.org/10.1007/s11240-011-0018-4.

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Park, Joonho, Thomas W. Okita, and Gerald E. Edwards. "Salt tolerant mechanisms in single-cell C4 species Bienertia sinuspersici and Suaeda aralocaspica (Chenopodiaceae)." Plant Science 176, no. 5 (May 2009): 616–26. http://dx.doi.org/10.1016/j.plantsci.2009.01.014.

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Lung, Shiu-Cheung, Makoto Yanagisawa, and Simon D. X. Chuong. "Protoplast isolation and transient gene expression in the single-cell C4 species, Bienertia sinuspersici." Plant Cell Reports 30, no. 4 (November 20, 2010): 473–84. http://dx.doi.org/10.1007/s00299-010-0953-2.

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Dissertations / Theses on the topic "Bienertia sinuspersici"

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Wimmer, Diana [Verfasser]. "Analysis of subcellular protein targeting mechanisms in the single-cell C4 species Bienertia sinuspersici / Diana Wimmer." Hannover : Technische Informationsbibliothek (TIB), 2017. http://d-nb.info/1137062479/34.

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Rosnow, Josh Jeffrey. "In vitro cultures and regeneration of Bienertia sinuspersici (Chenopodiaceae) under increasing concentrations of sodium chloride and carbon dioxide." Pullman, Wash. : Washington State University, 2010. http://www.dissertations.wsu.edu/Thesis/Spring2010/j_rosnow_042010.pdf.

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Thesis (M.S. in molecular plant sciences)--Washington State University, May 2010.
Title from PDF title page (viewed on July 9, 2010). "Department of Molecular Plant Sciences." Includes bibliographical references.
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Leisner, Courtney Price. "Salinity tolerance in the single-cell C₄ species Bienertia sinuspersici and the Kranz-type C₄ species Suaeda eltonica (Chenopodiaceae)." Pullman, Wash. : Washington State University, 2009. http://www.dissertations.wsu.edu/Thesis/Summer2009/c_leisner_080809.pdf.

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Thesis (M.S. in botany)--Washington State University, August 2009.
Title from PDF title page (viewed on Sept. 22, 2009). "Department of Biological Sciences." Includes bibliographical references (p. 138-139).
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Park, Joonho. "Spatial development of the cytoplasmic compartments for single cell C₄ photosynthesis, and mechanisms of tolerance to salinity in Bienertia sinuspersici." Online access for everyone, 2008. http://www.dissertations.wsu.edu/Dissertations/Spring2008/j_park_041608.pdf.

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Erlinghäuser, Matthias [Verfasser]. "Identifizierung von Komponenten der single-cell C4 Photosynthese in Bienertia sinuspersici durch vergleichende Transkriptomik photosynthetischer Gewebe und proteomische Untersuchung der Hüllmembranen dimorpher Chloroplasten / Matthias Erlinghäuser." Hannover : Gottfried Wilhelm Leibniz Universität Hannover, 2018. http://d-nb.info/1172414092/34.

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Hagenau, Lisa [Verfasser]. "Identification of regulators and effectors of single-cell C4 morphology by comparative transcriptomics of dark-grown cotyledons of the single-cell C4 plant Bienertia sinuspersici and the related C3 plant Suaeda heterophylla / Lisa Hagenau." Hannover : Technische Informationsbibliothek (TIB), 2017. http://d-nb.info/1169968937/34.

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Northmore, Jennifer Anne. "In vitro regeneration of Bienertia sinuspersici." Thesis, 2010. http://hdl.handle.net/10012/5477.

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This thesis presents a study on the development of plant regeneration protocols for Bienertia sinuspersici, one of three species of the family Chenopodiaceae that were discovered to perform C4 photosynthesis in individual chlorenchyma cells. Protocols for in vitro regeneration of B. sinuspersici were developed as follows: 1) vegetative propagation via adventitious root induction of cuttings was optimized; 2) direct organogenesis and plant regeneration using bud cultures was achieved; 3) plant regeneration via indirect organogenesis of stem-derived callus was established. All plant materials were obtained from mature, greenhouse grown B. sinuspersici plants and cultured in vitro on Murashige and Skoog (MS) basal media supplemented with phytohormones. Vegetative propagation by adventitious root formation of cuttings was examined using various strengths of MS medium as well as the effects of auxins [2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and ??-napthalene acetic acid (NAA)] on root development. Half-strength MS medium and IBA were most effective for adventitious root induction. Histological analysis of root anatomy demonstrated the various effects of individual auxin on root development. Direct organogenesis via bud culture for shoot multiplication and plant regeneration was established. Shoot multiplication occurred on medium supplemented with a cytokinin, 6-benzylaminopurine (BAP). Elongation of multiplied shoots was achieved using medium containing a lower concentration of BAP or gibberellic acid. Rooting of elongated shoots was attempted using IBA in vitro or ex vitro, and through micrografting. Rooted shoots were transplanted to soil, acclimated in the greenhouse, and transferred to a growth chamber. Plant regeneration via indirect organogenesis was also established. Stem explants were cultured on medium containing kinetin and 2,4-D to induce callus formation. Shoot organogenesis from callus tissue was obtained on medium containing thidiazuron (TDZ). In vitro-derived shoots were transferred to medium containing BAP for shoot multiplication, and subsequently elongated and rooted as described above. Histological analysis of leaves of regenerated plants from both direct and indirect organogenesis methods showed chlorenchyma cells have the distinctive feature, intracellular compartmentalization of organelles, of the single-cell C4 system. Immunoblot analysis of proteins isolated from leaves of regenerated plants detected key C4 photosynthetic enzymes, providing further evidence that single-cell C4 photosynthesis is present. Cytological analysis of root tips from in vitro-derived plants via indirect organogenesis verified normal diploid chromosome number (2n=18) in dividing cells, suggesting neither polyploidy nor aneuploidy occurred in regenerated plants. Reproducible methods of in vitro regeneration of Bienertia sinuspersici have been established for vegetative propagation from cuttings, direct organogenesis via axillary bud culture, and indirect organogenesis. These in vitro techniques would serve as useful tools for multiplication and genetic transformation of this plant species.
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Lung, Shiu Cheung. "Characterization of the chloroplast protein import receptors in the single-cell C4 species Bienertia sinuspersici." Thesis, 2012. http://hdl.handle.net/10012/6646.

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Bienertia sinuspersici is one of the three currently known land plants which perform C4 photosynthesis without the conventional dual-cell system, but rather through intracellular compartmentation of organelles and enzymes within individual photosynthetic cells. Whilst earlier works have involved anatomical and molecular techniques to understand the unique organelle partitioning, the lack of technology for gene manipulation and organelle purification has precluded biochemical characterization of this novel single-cell C4 model. Of particular interest is the unknown mechanism(s) leading to the differential accumulation of photosynthetic enzymes in the dimorphic chloroplasts within the same cells, which implicates the selective import of precursor proteins into the organelles. The first part of this thesis describes the establishment of multiple cell biology techniques for studying the single-cell C4 model. First, procedures have been optimized for isolating a homogenous population of chlorenchyma protoplasts from B. sinuspersici. Cell viability and preservation of the complex organelle compartmentation were confirmed by cytochemical staining and transient expression of fluorescent fusion proteins. The versatility of this highly efficient gene manipulation system was exemplified by the sorting of various fusion proteins to their respective subcellular locations. The isolation of intact chlorenchyma protoplasts has also led to the successful purification of the dimorphic chloroplasts. A protocol has been optimized for hypo-osmotic lysis of isolated protoplasts and concomitant separation of the central chloroplasts embedded in discrete ball structures and the peripheral chloroplasts adhered to the vacuole surface. The dimorphic chloroplasts were further purified to high homogeneity as evaluated by immunoblotting of their respective protein markers. The second part of this thesis reports the identification and characterization of the chloroplast protein import receptors Toc159 and Toc34 in B. sinuspersici. Protein expression profiling revealed independent regulation of two identified Toc159 isoforms during leaf development, implicating their different substrate selectivities. Several subcellular localization approaches consistently demonstrated the co-existence of a cytosolic form of Toc159, which was found to interact with actin filaments and microtubules. This unique localization pattern is pertinent to the concurrent discovery of a novel mechanism for the reversible targeting of Toc159 to the chloroplast surface. Multiple computational methods predicted a chloroplastic transit peptide-like sorting signal at the carboxyl end of Toc159, which was experimentally demonstrated to guide passenger proteins to the chloroplast envelope or stroma depending on its orientation. Collectively, the data in this thesis point to a chloroplast protein import model proposing that a novel sorting signal guides the movement of the Toc159 receptor to and from the chloroplast surface for preprotein targeting which may be assisted by the cytoskeleton. In light of the established gene manipulation and organelle purification techniques, the mechanism for selective protein import into dimorphic chloroplasts can be studied in the future.
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Yanagisawa, Makoto. "Expression of photosynthetic genes and possible regulatory mechanisms in the single-cell C4 species, Bienertia sinuspersici." Thesis, 2012. http://hdl.handle.net/10012/6588.

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Bienertia sinuspersici is one of three terrestrial plants identified to perform C4 photosynthesis in a single chlorenchyma cell by compartmentation of organelles and photosynthetic enzymes. This thesis describes a study on the distribution of photosynthetic proteins and their corresponding transcripts in an attempt to understand the regulatory mechanisms underlying their differential accumulation in two types of chloroplast. The patterns of photosynthetic enzymes and transcripts accumulation in developing leaves were examined by using immunolocalization and in situ hybridization. The polypeptides of Rubisco large subunit (RbcL) and pyruvate Pi dikinase (PPDK) accumulate equally in all chloroplasts before the formation of two intracellular cytoplasmic compartments: the central (CCC) and peripheral (PCC) cytoplasmic compartment. The differential accumulation of these enzymes is not completed until the mature stage, indicating that the transition from C3 to C4 photosynthesis occurs at the very late stage of leaf development. In mature chlorenchyma cells, RbcL accumulates 20-fold more in the CCC than in the PCC while PPDK demonstrates a concentration gradient that is lowest in chloroplasts in the center of the CCC and highest in the PCC chloroplasts. The pattern of rbcL transcript accumulation follows that of its polypeptides in developing leaves, suggesting the expression of this gene is controlled at the transcriptional and/or mRNA stability level. The quantitative real-time PCR results of rbcL transcripts from isolated chloroplasts of each compartment further supported this observation. Bioinformatics tools were used to predict possible structual motifs on rbcL mRNA as an attempt to speculate on their role in its distribution and identify the presence of secondary structures in the 5??? untranslated region (UTR) that may function in the regulation of gene expression. Detailed examination of the ultrastructure of the unique intracellular organization in the single-cell C4 system showed various vesicles in close proximity to chloroplasts in both compartments in mature chlorenchyma cells of B. sinuspersici under H2O2 treatments. To further understand the biogenesis of these vesicles, imunolocalization and transient expression of green fluorescent protein (GFP) experiments were performed. These studies identified stroma-filled tubules (stromules) as the structure that participates in the formation of sequestering vesicles (autophagosomes) containing cytosol and organelles. Fluorescent microscopic analyses using autophagosome-specific and autophagic vacuole markers only showed partial overlapping of two fluorescent signals. These results suggest that the formation of autophagosome and autophagic vacuole occur independently. The contribution of stromules to autophagosome formation and the involvement of chloroplastic envelopes in this process provide another level of regulation in the compartmentation of photosynthetic enzymes in single-cell C4 system. Collectively, the findings in this thesis enhance our overall understanding on the development and function of single-cell C4 photosynthesis.
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Conference papers on the topic "Bienertia sinuspersici"

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Jasim, Fatimah, and Fatima Abass. "Bienertia Sinuspersici Leaves Extracts Accelerated Wound Healing in laboratory animals." In Proceedings of the 1st International Multi-Disciplinary Conference Theme: Sustainable Development and Smart Planning, IMDC-SDSP 2020, Cyperspace, 28-30 June 2020. EAI, 2020. http://dx.doi.org/10.4108/eai.28-6-2020.2297924.

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