Relevant bibliographies by topics / Bifidobacterium bifidum

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  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers
  5. Reports

Academic literature on the topic 'Bifidobacterium bifidum'

Author: Grafiati
Published: 4 June 2021
Last updated: 16 February 2022

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Journal articles on the topic "Bifidobacterium bifidum"

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Anindita, Nosa Septiana, and Muslih Anwar. "Viabilitas Dan Aktivitas Antibakteri Bifidobacterium bifidum Dalam Susu Bifidus Dengan Suplementasi Ekstrak Cengkeh (Syzygium aromaticum)." agriTECH 41, no. 3 (August 20, 2021): 267. http://dx.doi.org/10.22146/agritech.40882.

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Pengembangan produk olahan susu kambing terfermentasi dan diperkaya dengan probiotik Bifidobacterium bifidum merupakan salah satu produk pangan fungsional (Functional Food) yang selanjutnya disebut sebagai Susu Bifidus. Susu bifidus sebagai salah satu produk fermentasi susu dapat dikonsumsi oleh masyarakat yang mengalami kesulitan mencerna laktosa (lactose intolerance). Daya terima konsumen terhadap susu kambing masih tergolong rendah dibandingkan dengan susu sapi, sehingga hal tersebut juga perlu diantisipasi. Penambahan ekstrak cengkeh (Syzygium aromaticum) pada susu Bifidus dapat digunakan sebagai alternatif senyawa aromatik alami, untuk menghilangkan aroma goaty pada susu kambing yang kurang begitu disukai oleh konsumen. Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi ekstrak cengkeh terhadap viabilitas (daya hidup) dan aktivitas antibakteri Bifidobacterium bifidum serta kualitas kimia susu Bifidus. Parameter yang diamati pada penelitian adalah total Bifidobacterium bifidum, total bakteri dan luas zona bening susu Bifidus. Suplementasi ekstrak cengkeh sebesar 2% sebagai bahan suplementasi pada Bifidus milk dapat meningkatkan viabilitas probiotik Bifidobacterium bifidum. Ekstrak cengkeh dalam produk Bifidus milk berperan sebagai agen preservatif, didukung dengan adanya aktivitas antibakteri terhadap Staphylococcus aureus dan Salmonella typhimurium dan penurunan total bakteri pada Bifidus milk.
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Peirotén, A., J. L. Arqués, M. Medina, and E. Rodríguez-Mínguez. "Bifidobacterial strains shared by mother and child as source of probiotics." Beneficial Microbes 9, no. 2 (February 27, 2018): 231–38. http://dx.doi.org/10.3920/bm2017.0133.

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Importance of bifidobacteria as part of the infant intestinal microbiota has been highlighted. Their acquisition is influenced by the mode of birth and the feed regime afterwards, with a special role of the maternal microbiota. The presence of the same shared bifidobacterial strains between breast milk and infant faeces in 14 mother-infant pairs was assessed by means of pulsed-field gel electrophoresis (PFGE) genotyping. Four shared strains of Bifidobacterium breve (2), Bifidobacterium longum subsp. infantis and B. longum subsp. longum were found in breast milk-infant faeces pairs. Two years later, a second survey yielded four shared strains of the species Bifidobacterium adolescentis, Bifidobacterium bifidum, B. longum subsp. longum and Bifidobacterium pseudocatenulatum. Moreover, a B. bifidum strain was found to be shared by the infant faeces of the first study and the mother faeces tested two years later, pointing out a long term persistence. Some of the selected bifidobacterial strains showed probiotic potential due to their survival to gastrointestinal conditions and their ability to form biofilms.
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Moubareck, C., M. Lecso, E. Pinloche, M. J. Butel, and F. Doucet-Populaire. "Inhibitory Impact of Bifidobacteria on the Transfer of β-Lactam Resistance among Enterobacteriaceae in the Gnotobiotic Mouse Digestive Tract." Applied and Environmental Microbiology 73, no. 3 (November 22, 2006): 855–60. http://dx.doi.org/10.1128/aem.02001-06.

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ABSTRACT While looking for new means to limit the dissemination of antibiotic resistance, we evaluated the role of potentially probiotic bifidobacteria on the transfer of resistance genes between enterobacteria. Transfers of bla genes encoding extended-spectrum β-lactamases (SHV-5 and CTX-M-15) were studied in the absence or presence of bifidobacteria. In vitro, transfer frequencies of these bla genes decreased significantly in the presence of three of five tested strains, i.e., Bifidobacterium longum CUETM-89-215, Bifidobacterium bifidum CIP-56.7T, and Bifidobacterium pseudocatenulatum CIP-104168T. Four transfer experiments were conducted in the digestive tract of gnotobiotic mice, the first three observing the effect of B. longum CUETM-89-215, B. bifidum CIP-56.7T, and B. pseudocatenulatum CIP-104168T on bla SHV-5 transfer and the fourth experiment studying the effect of B. bifidum CIP-56.7T on bla CTX-M-15 transfer. These experiments revealed significant decreases in the transconjugant levels (up to 3 logs) in mice having received B. bifidum CIP-56.7T or B. pseudocatenulatum CIP-104168T compared to control mice. Bifidobacteria appear to have an inhibitory impact on the transfer of antibiotic resistance genes. The inhibitory effect is associated to specific bifidobacterial strains and may be related to the production of thermostable metabolites by these strains.
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Turroni, Francesca, Elena Foroni, Paola Pizzetti, Vanessa Giubellini, Angela Ribbera, Paolo Merusi, Patrizio Cagnasso, et al. "Exploring the Diversity of the Bifidobacterial Population in the Human Intestinal Tract." Applied and Environmental Microbiology 75, no. 6 (January 23, 2009): 1534–45. http://dx.doi.org/10.1128/aem.02216-08.

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ABSTRACT Although the health-promoting roles of bifidobacteria are widely accepted, the diversity of bifidobacteria among the human intestinal microbiota is still poorly understood. We performed a census of bifidobacterial populations from human intestinal mucosal and fecal samples by plating them on selective medium, coupled with molecular analysis of selected rRNA gene sequences (16S rRNA gene and internally transcribed spacer [ITS] 16S-23S spacer sequences) of isolated colonies. A total of 900 isolates were collected, of which 704 were shown to belong to bifidobacteria. Analyses showed that the culturable bifidobacterial population from intestinal and fecal samples include six main phylogenetic taxa, i.e., Bifidobacterium longum, Bifidobacterium pseudocatenulatum, Bifidobacterium adolescentis, Bifidobacterium pseudolongum, Bifidobacterium breve, and Bifidobacterium bifidum, and two species mostly detected in fecal samples, i.e., Bifidobacterium dentium and Bifidobacterium animalis subp. lactis. Analysis of bifidobacterial distribution based on age of the subject revealed that certain identified bifidobacterial species were exclusively present in the adult human gut microbiota whereas others were found to be widely distributed. We encountered significant intersubject variability and composition differences between fecal and mucosa-adherent bifidobacterial communities. In contrast, a modest diversification of bifidobacterial populations was noticed between different intestinal regions within the same individual (intrasubject variability). Notably, a small number of bifidobacterial isolates were shown to display a wide ecological distribution, thus suggesting that they possess a broad colonization capacity.
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Turroni, Francesca, Sabrina Duranti, Christian Milani, Gabriele Andrea Lugli, Douwe van Sinderen, and Marco Ventura. "Bifidobacterium bifidum: A Key Member of the Early Human Gut Microbiota." Microorganisms 7, no. 11 (November 9, 2019): 544. http://dx.doi.org/10.3390/microorganisms7110544.

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Bifidobacteria typically represent the most abundant bacteria of the human gut microbiota in healthy breast-fed infants. Members of the Bifidobacterium bifidum species constitute one of the dominant taxa amongst these bifidobacterial communities and have been shown to display notable physiological and genetic features encompassing adhesion to epithelia as well as metabolism of host-derived glycans. In the current review, we discuss current knowledge concerning particular biological characteristics of the B. bifidum species that support its specific adaptation to the human gut and their implications in terms of supporting host health.
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Sakurai, Takuma, Toshitaka Odamaki, and Jin-zhong Xiao. "Production of Indole-3-Lactic Acid by Bifidobacterium Strains Isolated fromHuman Infants." Microorganisms 7, no. 9 (September 11, 2019): 340. http://dx.doi.org/10.3390/microorganisms7090340.

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Recent studies have shown that metabolites produced by microbes can be considered as mediators of host-microbial interactions. In this study, we examined the production of tryptophan metabolites by Bifidobacterium strains found in the gastrointestinal tracts of humans and other animals. Indole-3-lactic acid (ILA) was the only tryptophan metabolite produced in bifidobacteria culture supernatants. No others, including indole-3-propionic acid, indole-3-acetic acid, and indole-3-aldehyde, were produced. Strains of bifidobacterial species commonly isolated from the intestines of human infants, such as Bifidobacterium longum subsp. longum, Bifidobacterium longum subsp. infantis, Bifidobacterium breve, and Bifidobacterium bifidum, produced higher levels of ILA than did strains of other species. These results imply that infant-type bifidobacteria might play a specific role in host–microbial cross-talk by producing ILA in human infants.
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Sakurai, T., A. Yamada, N. Hashikura, T. Odamaki, and J. Z. Xiao. "Degradation of food-derived opioid peptides by bifidobacteria." Beneficial Microbes 9, no. 4 (June 15, 2018): 675–82. http://dx.doi.org/10.3920/bm2017.0165.

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Some food-derived opioid peptides have been reported to cause diseases, such as gastrointestinal inflammation, celiac disease, and mental disorders. Bifidobacterium is a major member of the dominant human gut microbiota, particularly in the gut of infants. In this study, we evaluated the potential of Bifidobacterium in the degradation of food-derived opioid peptides. All strains tested showed some level of dipeptidyl peptidase activity, which is thought to be involved in the degradation of food-derived opioid peptides. However, this activity was higher in bifidobacterial strains that are commonly found in the intestines of human infants, such as Bifidobacterium longum subsp. longum, B. longum subsp. infantis, Bifidobacterium breve and Bifidobacterium bifidum, than in those of other species, such as Bifidobacterium animalis and Bifidobacterium pseudolongum. In addition, some B. longum subsp. infantis and B. bifidum strains showed degradative activity in food-derived opioid peptides such as human and bovine milk-derived casomorphin-7 and wheat gluten-derived gliadorphin-7. A further screening of B. bifidum strains revealed some bifidobacterial strains that could degrade all three peptides. Our results revealed the potential of Bifidobacterium species in the degradation of food-derived opioid peptides, particularly for species commonly found in the intestine of infants. Selected strains of B. longum subsp. infantis and B. bifidum with high degradative capabilities can be used as probiotic microorganisms to eliminate food-derived opioid peptides and contribute to host health.
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Young, Sarah L., Mary A. Simon, Margaret A. Baird, Gerald W. Tannock, Rodrigo Bibiloni, Kate Spencely, Juliette M. Lane, et al. "Bifidobacterial Species Differentially Affect Expression of Cell Surface Markers and Cytokines of Dendritic Cells Harvested from Cord Blood." Clinical Diagnostic Laboratory Immunology 11, no. 4 (July 2004): 686–90. http://dx.doi.org/10.1128/cdli.11.4.686-690.2004.

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ABSTRACT The gut microbiota may be important in the postnatal development of the immune system and hence may influence the prevalence of atopic diseases. Bifidobacteria are the most numerous bacteria in the guts of infants, and the presence or absence of certain species could be important in determining the geographic incidence of atopic diseases. We compared the fecal populations of bifidobacteria from children aged 25 to 35 days in Ghana (which has a low prevalence of atopy), New Zealand, and the United Kingdom (high-prevalence countries). Natal origin influenced the detection of bifidobacterial species in that fecal samples from Ghana almost all contained Bifidobacterium infantis whereas those of the other children did not. Choosing species on the basis of our bacteriological results, we tested bifidobacterial preparations for their effects on cell surface markers and cytokine production by dendritic cells harvested from cord blood. Species-specific effects on the expression of the dendritic-cell activation marker CD83 and the production of interleukin-10 (IL-10) were observed. Whereas CD83 expression was increased and IL-10 production was induced by Bifidobacterium bifidum, Bifidobacterium longum, and Bifidobacterium pseudocatenulatum, B. infantis failed to produce these effects. We concluded that B. infantis does not trigger the activation of dendritic cells to the degree necessary to initiate an immune response but that B. bifidum, B. longum, and B. pseudocatenulatum induce a Th2-driven immune response. A hypothesis is presented to link our observations to the prevalence of atopic diseases in different countries.
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Mart�n, Roc�o, Esther Jim�nez, Hans Heilig, Leonides Fern�ndez, Mar�a L. Mar�n, Erwin G. Zoetendal, and Juan M. Rodr�guez. "Isolation of Bifidobacteria from Breast Milk and Assessment of the Bifidobacterial Population by PCR-Denaturing Gradient Gel Electrophoresis and Quantitative Real-Time PCR." Applied and Environmental Microbiology 75, no. 4 (December 16, 2008): 965–69. http://dx.doi.org/10.1128/aem.02063-08.

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ABSTRACT The objective of this work was to elucidate if breast milk contains bifidobacteria and whether they can be transmitted to the infant gut through breastfeeding. Twenty-three women and their respective infants provided samples of breast milk and feces, respectively, at days 4 to 7 after birth. Gram-positive and catalase-negative isolates from specific media with typical bifidobacterial shapes were identified to the genus level by F6PPK (fructose-6-phosphate phosphoketolase) assays and to the species level by 16S rRNA gene sequencing. Bifidobacterial communities in breast milk were assessed by PCR-denaturing gradient gel electrophoresis (PCR-DGGE), and their levels were estimated by quantitative real-time PCR (qRTi-PCR). Bifidobacteria were present in 8 milk samples and 21 fecal samples. Bifidobacterium breve, B. adolescentis, and B. bifidum were isolated from milk samples, while infant feces also contained B. longum and B. pseudocatenulatum. PCR-DGGE revealed the presence of one to four dominant bifidobacterial bands in 22 milk samples. Sequences with similarities above 98% were identified as Bifidobacterium breve, B. adolescentis, B. longum, B. bifidum, and B. dentium. Bifidobacterial DNA was detected by qRTi-PCR in the same 22 milk samples at a range between 40 and 10,000 16S rRNA gene copies per ml. In conclusion, human milk seems to be a source of living bifidobacteria for the infant gut.
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Ročková, Š., V. Rada, J. Havlík, R. Švejstil, E. Vlková, V. Bunešová, K. Janda, and I. Profousová. "Growth of bifidobacteria in mammalian milk." Czech Journal of Animal Science 58, No. 3 (March 4, 2013): 99–105. http://dx.doi.org/10.17221/6666-cjas.

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Microbial colonization of the mammalian intestine begins at birth, when from a sterile state a newborn infant is exposed to an external environment rich in various bacterial species. An important group of intestinal bacteria comprises bifidobacteria. Bifidobacteria represent major intestinal microbiota during the breast-feeding period. Animal milk contains all crucial nutrients for babies’ intestinal microflora. The aim of our work was to test the influence of different mammalian milk on the growth of bifidobacteria. The growth of seven strains of bifidobacteria in human milk, the colostrum of swine, cow’s milk, sheep’s milk, and rabbit’s milk was tested. Good growth accompanied by the production of lactic acid was observed not only in human milk, but also in the other kinds of milk in all three strains of Bifidobacterium bifidum of different origin. Human milk selectively supported the production of lactic acid of human bifidobacterial isolates, especially the Bifidobacterium bifidum species. The promotion of bifidobacteria by milk is species-specific. Human milk contains a key factor for the growth of specific species or strains of human-origin bifidobacteria compared to other kinds of milk. In contrast, some components (maybe lysozyme) of human milk inhibited the growth of Bifidobacterium animalis. Animal-origin strains of bifidobacteria were not able to significantly grow even in milk of animal origin, with the exception of B. animalis subsp. lactis 1,2, which slightly grew in sheep’s milk.
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Dissertations / Theses on the topic "Bifidobacterium bifidum"

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Boutry, Etienne. "Exoglycosidases de Bifidobacterium bifidum souche AA 2/2." Lille 1, 1989. http://www.theses.fr/1989LIL10163.

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La production de beta-d-galactosidase (EC. 3. 2. 1. 23), de n-acetyl-beta-d-glucosaminidase (EC. 3. 2. 1. 30), de neuraminidase (EC. 3. 2. 1. 18) et d'alpha-l-fucosidase (EC. 3. 2. 1. 51) a été optimisée par fermentation. La bactérie anaérobie Bifidobacterium bifidum souche AA/22 a été cultivée en milieu liquide renfermant par litre : 37 g d'un extrait sec de cerveau-cœur, 10 g de glucose et des facteurs bifidigènes (1 g de gynolactose ou 66 ml de lait de femme). La fermentation est conduite sous N2/CO2 à 37°C et à ph 6,8. L'extraction des systèmes enzymatiques par une technique aux ultra-sons a été optimisée en tampon phosphate de sodium 20 mm ph 6,8 renfermant de l'EDTA 20 mm et 0,1 p. 100 de nonidet P 40. Après centrifugation d'une heure à 100 000 g la solution enzymatique obtenue renferme: 7 U de beta-d-galactosidase, 1,6 U de n-acetyl-beta-d-glucosaminidase, 90 mU de neuraminidase et 400 mU de fucosidase par mg de protéine. Une neuraminidase a été purifiée jusqu'à homogénéité en électrophorèse dénaturante (SDS). La masse moléculaire de l'enzyme native est de 75 kDa, elle est constituée par deux sous-unités identiques (35 kDa). Le phi, la température et le ph optimum d'action sont respectivement de 4,2, 50°C et 5,0. Cette neuraminidase est sensible à l'action de la température, elle n'est stable qu'en dessous de 48°C. L'activité est indépendante des métaux. Elle permet l'hydrolyse des liaisons alpha 2,3 plus rapidement que les alpha 2,6 et les alpha 2,8. L'enzyme agit aussi bien sur les glycopeptides que sur les glycoprotéines natives.
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Hassi, Chafiq. "Contribution a l'etude d'utilisation de la n-acetyl-beta-d-glucosamine par b. Bifidum atcc 15696 et b. Animalis atcc 25527 : preparation, purification et caracterisation de leur n-acetyl-beta-d-glucosaminidase." Lille 2, 1994. http://www.theses.fr/1994LIL2P253.

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Krzewinski, Frédéric. "Transport et métabolisme des saccharides chez Bifidobacterium bifidum SM 20082." Lille 1, 1997. http://www.theses.fr/1997LIL10005.

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Nous avons etudie la capacite de bifidobacterium bifidum dsm 20082 a assimiler des saccharides. Cette etude a ete realisee en utilisant la technique de microcalorimetrie. Les resultats obtenus montrent que bifidobacterium bifidum est capable de croitre sur milieu riche (tpy) en presence ou en absence de saccharides. Sur milieu semi-synthetique (garches modifie), seules les n-acetylhexosamines permettent une croissance bacterienne, elles sont donc assimilees comme seule source de carbone. De plus, la quantite de chaleur degagee est proportionnelle a la consommation du saccharide. Les facteurs bifidogenes ne sont en realite que des nutriments preferentiels des bifidobacteries. Nous nous sommes interesses ensuite aux mecanismes d'incorporation de differents saccharides, glucose, fructose, galactose, n-acetylglucosamine, lactose et lacto-n-biose. La determination du mecanisme de transport de chaque saccharide a ete realisee a l'aide d'inhibiteur de translocation et de l'etude de la phosphorylation des saccharides. Les six mecanismes de transport se sont averes etre constitutifs. Le saccharide utilise lors de la culture n'entraine qu'une adaptation de la bacterie au saccharide, voire aucun changement dans la capacite d'incorporation. Les systemes de transport montrent une grande specificite vis-a-vis du saccharide qu'ils transportent. Le glucose est vehicule par un symport a cation regule par les ions potassium, avant d'etre phosphoryle par une glucokinase atp-independante. Le galactose traverserait la membrane par un mecanisme de diffusion. La galactokinase est activee en presence d'atp. Le lacto-n-biose et le lactose sont incorpores par des symports a proton. La -d-galactosidase est presente en grande quantite au niveau intracellulaire afin de liberer le glucose et le galactose. Le fructose est vehicule par un symport a proton. La fructokinase voit son activite diminuer de 60% en presence d'atp. Bifidobacterium bifidum aurait trouve a ce niveau un moyen de reguler le transport du fructose par un mecanisme de retro-inhibition.
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Westermann, Christina [Verfasser]. "Analysis of potential host-colonization factors in Bifidobacterium bifidum S17 / Christina Westermann." Ulm : Universität Ulm. Fakultät für Naturwissenschaften, 2015. http://d-nb.info/1078674221/34.

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Appourchaux, Laurence. "Purification et propriétés des béta-D-galactosidases des N-acétyl-béta-D-glucosaminidases de Bifidobacterieum bifidum souche AA 2/2." Lille 1, 1989. http://www.theses.fr/1989LIL10164.

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Quatre activités exoglycosidasiques de bifidobacterium bifidum souche AA/22 ont été étudiées. Il s'agit des activités: béta-galactosidase, n-acétyl-beta-hexosaminidase, alpha-neuraminidase et alpha-fucosidase. Ces activités sont endo-cellulaires, leur extraction est réalisée par ultra-sons. Le schéma de purification suivi nous a permis d'obtenir six fractions. Trois fractions ne contenant qu'une seule activité : une alpha-neuraminidase et deux beta-galactosidases. Trois fractions possèdent chacune deux activités : une béta-galactosidase et une alpha-fucosidase ; une alpha-neuraminidase et une n-acétyl-béta-hexosaminidase ; une béta-galactosidase et une n-acétyl-béta-hexosaminidase. Toutes ces fractions ne révèlent qu'une seule bande en électrophorèse non dénaturante. Nous avons vérifié que celles-ci correspondaient bien aux activités suivies. Les deux activités béta-galactosidase purifiées séparément sont homogènes en électrophorèse dénaturante. Toutes ces activités ont des températures optimales comprises entre 40 et 48°C. Les pH optimaux sont compris entre 5 et 6. 5 et les masses moléculaires relatives déterminées en gel filtration sur Superose 6 varient de 97000 à 420000. Les pHi sont acides (4 a 5. 1). Seule une activité béta-galactosidase est sensible à l'EDTA et à l'EGTA, de plus Elle est activée par le calcium et le magnésium. Les paramètres enzymatiques des béta-galactosidases ont été réalisés sur le pNP-Gal et sur le lactose, ils mettent en évidence trois groupes de réactivité différente. Tous ces paramètres nous permettent de conclure que Bifido bacterium bifidum souche AA/22 possède au moins trois béta-galactosidases différentes. Nous n'avons pas prouvé que dans les fractions contenant deux activités, nous étions en présence d'un complexe enzymatique. Mais nous avons montré que la présence d'une activité influençait la réactivité de l'autre activité présente.
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Goulas, Theodoros K. "Biological and biotechnological aspects of β- and α-galactosidases from Bifidobacterium bifidum ncimb41171." Thesis, University of Reading, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.494990.

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In this thesis, the field of the bifidobacteria! physiology in relation to β- and α-galactosidases is described and perspectives of them for oligosaccharides synthesis are discussed- The experimental work focused on the isolation and characterisation of these cell constituents from Bifidobacterium bifidum NCIMB41171. To generate a basis for subsequent work the construction of a genomic DNA library was carried out. This resulted in the isolation of four different β-galactosidases (bbgI, bbgII, bbgIII and bbgIV) and one α-galactosidase (melA) genes. Molecular characterisation of the gene products indicated their putative location, which was subsequently confirmed after expression in an E. coli host. Nucleotide and amino acid sequence comparisons with other known bifidobacterial enzymes revealed aspects of either their evolution or conservation among species.
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Teixeira, Gabriela Luciana Santos Bastos. "Qualidade e Viabilidade de Requeijão Cremoso Adicionado de Lactobacillus Acidophilus e Bifidobacterium Bifidum." Universidade Federal de Pernambuco, 2012. https://repositorio.ufpe.br/handle/123456789/11725.

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A adição de culturas probióticas tem sido testada em vários produtos lácteos, e a maior parte dos microrganismos consegue permanecer viável, alcançando características desejáveis no produto final. O objetivo da pesquisa foi desenvolver requeijão cremoso com probióticos, Lactobacillus acidophilus e Bifidobacterium bifidum, a fim de se tornar uma alternativa saudável de um alimento de amplo consumo. Foram elaboradas 3 formulações: requeijão cremoso fresco padrão, requeijão cremoso fresco com probióticos, requeijão cremoso fresco probiótico com reduzido teor de gordura. Para a caracterização do produto foram realizadas análises para determinação da composição centesimal, em triplicata para determinação de umidade, cinzas, lipídios e proteínas, e carboidratos. A contagem de coliformes totais e Escherichia coli foram realizadas nas amostras para análise da qualidade higiênico-sanitária. Realizou-se a contagem de L. acidophilus e B. bifidum durante o período de armazenamento de 15 dias. A avaliação da resistência das culturas probióticas às condições digestivas foi realizada empregando-se um modelo in vitro, utilizando suco gástrico e entérico simulados e enzimas do trato gastrintestinal. A análise da cor foi realizada por colorimetria em escala CIELab. A análise sensorial foi aplicada pelo teste de aceitação por escala hedônica para avaliação dos atributos e avaliação da qualidade global e intenção de compra. A composição centesimal dos requeijões padrão e adicionado de probióticos atendeu aos requisitos da legislação para o produto. O requeijão proposto como light apresentou redução de 26% no valor calórico total e 43% no teor de lipídeos em relação ao padrão. Quanto a acidez titulável, observou-se que o armazenamento influenciou significativamente os seus valores nas preparações com probióticos, diferindo do padrão que não apresentou alterações nos valores durante o armazenamento. A qualidade higênico-sanitária foi satisfatória, visto que as amostras foram negativas para presença de coliformes totais e Escherichia coli. As contagens individuais de Lactobacillus acidophilus e Bifidobacterium bifidum, estiveram de acordo com as exigências da legislação para alimentos com alegações de propriedades funcionais. O requeijão cremoso fresco probiótico apresentou contagem de L. acidophilus de 4,0x107UFC/g e B. bifidum de 2,0x108UFC/g aos sete dias de armazenamento e o requeijão cremoso fresco probiótico com reduzido teor de gordura apresentou contagem de L. acidophilus de 5,0x109UFC/g e B. bifidum de 2,0x109UFC/g. A redução de gordura no produto até os níveis estudados não interferiu na viabilidade dos probióticos no requeijão cremoso durante o armazenamento de 15 dias. Após a simulação da digestão in vitro, houve redução da população total de probióticos em ambas as formulações com resultados mais expressivos para a formulação com reduzido teor de gordura afirmando o efeito protetor das gorduras para a sobrevivência dos probióticos ao trato gastrintestinal. A análise instrumental da cor dos requeijões cremosos indicou produtos com boa luminosidade, com acentuação da cor amarela ao longo do tempo de armazenamento. A avaliação sensorial demonstrou que a adição de probióticos resultaram em requeijões cremosos com melhor perfil de características e maior aceitabilidade e intenção de compra que a preparação padrão. Pode-se concluir que os probióticos melhoraram a qualidade sensorial do produto, sendo tais amostras preferidas pelos julgadores. O requeijão cremoso tradicional e com baixo teor de gordura são tecnologicamente viáveis para a incorporação das culturas probióticas de L. acidophilus e B. bifidum.
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Possamai, Maikel. "Desempenho, metabolismo e microbiota intestinal de leitões alimentados com rações contendo probióticos e simbióticos." Universidade Estadual do Oeste do Paraná, 2010. http://tede.unioeste.br:8080/tede/handle/tede/1614.

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Two experiments were conducted to evaluate the effect of using probiotics and symbiotic in piglets diets in the nursery phase. The first experiment was the performance of piglets from 21 to 35, from 36 to 49 days and in total period, being also held the count of lactic bacteria, fecal coliforms and clostridia in the piglets faeces at 35 and 49 days old. The economic viability of probiotics and symbiotic used in piglets diets was also evaluated. There were used 120 piglets, with 21 days old, with initial average weight of 5.80 ± 0.30 kg, distributed in a randomized blocks design, in a factorial scheme consisting of two levels of probiotics (0.30 and 0.60%) and three levels of inulin inclusion (0.00; 0.25 and 0.50%), totalizing six treatments with five replicates. In the metabolism trial were used 24 barrows, with average initial weight of 18.00 ± 0.38 kg, distributed individualy in metabolic cages, and the experimental design and treatments used were the same described in the performance experiment, with four replicates. In this trial were determined the values of digestible energy, metabolizable energy, the digestibility and metabolizability coefficients of gross energy and at the end of metabolism trial, were collected fecal samples, by means of retal massage to determine fecal pH. The inclusion of probiotic and inulin in the diets did not influence (P>0.05) the performance of piglets, from 21 to 35 and from 21 to 49 days of age, the cost index, the index of economic efficiency, the count of lactic bacteria, fecal coliforms and clostridia in the faeces. In the metabolism trial was observed that the inclusion of probiotics in the diets did not influence in the energy metabolization. Nevertheless, the levels of inclusion of inulin reduced (P<0.05) the digestible energy and the digestibility coefficient of gross energy, but did not influence in the coefficient of gross energy metabolization. The pH fecal values were not influenced by the inclusion of probiotic and symbiotic. The use of probiotic and inulin, and their association, did not change the performance, thefaeces microbial counts, the economic viability and gross energy metabolization of piglets from 21 to 49 days old
Foram realizados dois experimentos para avaliar o efeito do uso de probióticos e simbiótico em rações para leitões na fase de creche. O primeiro experimento foi o de desempenho dos leitões dos 21 aos 35, dos 36 aos 49 dias e no período total, sendo também realizada a contagem das bactérias lácticas, coliformes e clostrídios nas fezes dos leitões, aos 35 e 49 dias de idade. A viabilidade econômica da utilização dos probióticos e simbiótico nas rações dos leitões também foi avaliada. Foram utilizados 120 leitões, com 21 dias de idade, com peso médio inicial de 5,80 ± 0,30 kg, distribuídos em um delineamento experimental de blocos ao acaso, em um esquema fatorial constituído de dois níveis de probióticos (0,30 e 0,60%) e três níveis de inclusão de inulina (0,00; 0,25 e 0,50%), totalizando seis tratamentos com cinco repetições. No ensaio de metabolismo foram utilizados 24 suínos machos castrados, com peso vivo médio inicial de 18,00 ± 0,38 kg, distribuídos individualmente em gaiolas de metabolismo, e o delineamento experimental e tratamentos utilizados foram os mesmos descritos no experimento de desempenho, com quatro repetições. Foram determinados os valores de energia digestível, energia metabolizável, os coeficientes de digestibilidade e metabolizibilidade da energia bruta e no final do ensaio metabólico foram coletadas amostras de fezes, por meio de massagem retal, para determinar o pH fecal. A inclusão do probiótico e inulina nas rações não influenciou (P>0,05) o desempenho dos leitões, dos 21 aos 35 e dos 21 aos 49 dias de idade, o índice de custo, o índice de eficiência econômica, a contagem de bactérias acidoláticas, coliformes e clostrídios das fezes. No ensaio metabólico foi observado que a inclusão de probiótico nas rações não influenciou na metabolizibilidade da energia. No entanto, os níveis de inclusão de inulina reduziram (P<0,05) os valores de energia digestível e o coeficiente de digestibilidade da energia bruta, mas não influenciaram no coeficiente de metabolizibilidade da energia bruta. Os valores de pH fecal não foram influenciados pela inclusão de probiótico e simbiótico. O uso de probiótico e inulina, e sua associação, não alteraram o desempenho, a contagem de micro-organismos, a viabilidade econômica e a metabolizibilidade da energia bruta de leitões dos 21 aos 49 dias de idade
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Chou, Lan-Szu. "Isolation and Biochemical Characterization of Acid- and Bile- Tolerant Strains of Lactobacillus Acidophilus and Bifidobacterium Bifidum." DigitalCommons@USU, 1997. https://digitalcommons.usu.edu/etd/5427.

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Lactic acid bacteria have been reported to be used as a health adjunct in food for u many years. However, these health benefits have not been proven. and how these bacteria pass through the digestion process and remain viable in the human intestinal tract is still not clear. The aim of this work was to isolate mutants from Lactobacillus acidophilus or Bifidobacterium bifidum that could tolerate the conditions of the digestion process (low pH and bile conduction) and to characterize these isolated mutants. Acid- and bile-tolerant mutants of L. acidophilus were isolated from parental strains successfully using natural selection techniques. These mutants survived and grew at conditions of pH 3.5 with 0.2% mixed bile salts added. After the selection, phenotypic characterization was identified to further clarify desirable traits for use as probiotic adjuncts in foods. These phenotypic characteristics included protease, aminopeptidase, ß-galactosidase, and bile salt hydrolase activity. Based on different protease, aminopeptidase, and ß-galactosidase activity, selected acid- and bile-tolerant mutants contained different growth characteristics compared with their parents. All the isolates tested showed different bile salt hydrolase activity, and this activity was not strain and medium dependent. Plasmid profiles and fatty acid analysis were conducted to provide more information of these acid/bile tolerant isolates and whether or not they were mutants from their parent strains rather than only adapted variants. Results showed the acid-/bile-tolerant isolates contained different plasmid profiles and cell wall fatty acids compared with their parents, which indicated these isolates were mutants. Protein expression by two-dimensional gel electrophoresis showed different protein expression patterns between acid- and bile-tolerant mutants and their parents. fm1her suggesting these isolates were mutants. We observed the protein production in parent strains decreased as the pH decreased. and protein expression in mutants remained the same as pH decreased. Two of the proposed health benefits of probiotic bacteria are anticholesterol activity and antimicrobial activity. These were evaluated using selected acid- and bile-tolerant mutants. Results showed no decrease of cholesterol in the test medium during bacterial growth. The observed antimicrobial activity was due to the presence of active cells. and this may relate to the acid production during cell growth and not to the production of antimicrobial substances. We concluded that the acid-/bile-tolerant isolates were mutants, and they survived and grew better in harsh environments compared with their parent strains. These mutants may be useful as a food adjunct in the future, but further study is needed to establish their use and possible probiotic benefits.
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Sun, Zhongke [Verfasser]. "Development of gene expression systems in Bifidobacterium bifidum S17 and their application for tumor therapy / Zhongke Sun." Ulm : Universität Ulm. Fakultät für Naturwissenschaften, 2014. http://d-nb.info/1062611020/34.

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Book chapters on the topic "Bifidobacterium bifidum"

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Quigley, E. M. M. "Bifidobacterium bifidum." In The Microbiota in Gastrointestinal Pathophysiology, 131–33. Elsevier, 2017. http://dx.doi.org/10.1016/b978-0-12-804024-9.00014-8.

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"Gastroprotective Effects of Bifidobacterium bifidum BF-1 and B. bifidum Strain Yakult." In Beneficial Microbes in Fermented and Functional Foods, 342–59. CRC Press, 2014. http://dx.doi.org/10.1201/b17912-23.

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Hossein Khani, Ali, Seyed Milad Mousavi Jazayeri, Elahe Ebrahimi, and Ayoub Farhadi. "The Bifidobacterim bifidum (BIB2) Probiotic Increased Immune System Factors in Men Sprint Athletes." In Nutraceuticals - Past, Present and Future. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.84222.

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Conference papers on the topic "Bifidobacterium bifidum"

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He, Chen, Hu Man, Shu Guowei, Ma Qi, and Qin Tao. "Effect of prebiotics on growth of bifidobacterium bifidum." In 2011 International Conference on Human Health and Biomedical Engineering (HHBE). IEEE, 2011. http://dx.doi.org/10.1109/hhbe.2011.6028988.

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Dorta, Claudia, Anna Claudia Sahade Brunatti, Flávia Maria Vasques Farinazzi-Machado, Vanessa Pachelle Simão, and Ariele Cristina Viana dos Santos. "Sorvete Sabor Morango com Adição do Probiótico Bifidobacterium Bifidum ou Lactobacillus Casei." In XII Latin American Congress on Food Microbiology and Hygiene. São Paulo: Editora Edgard Blücher, 2014. http://dx.doi.org/10.5151/foodsci-microal-001.

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Shu, Guowei, Man Hu, He Chen, Zhe Ji, Tao Qin, and Qi Ma. "Notice of Retraction: Effect of the Extract of Fructus tribuli on Growth of Bifidobacterium bifidum." In 2011 5th International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5780695.

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Shigematsu, Elke, Claudia Dorta, Ingrid Tagliamento Ferioli Pereira, Eliza Lima da Silva, Silvana Pedroso de Góes-Favoni, and Marina Felix Cedran. "Manutenção da viabilidade celular de Bifidobacterium bifidum após adição sorvete à base de soja sabor chocolate." In Simpósio Nacional de Bioprocessos e Simpósio de Hidrólise Enzimática de Biomassa. Campinas - SP, Brazil: Galoá, 2015. http://dx.doi.org/10.17648/sinaferm-2015-33322.

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Susilowati, Agustine, Aspiyanto, and Muhammad Ghozali. "Drying process of fermented inulin fiber concentrate by Bifidobacterium bifidum as a dietary fiber source for cholesterol binder." In PROCEEDINGS OF THE 3RD INTERNATIONAL SYMPOSIUM ON APPLIED CHEMISTRY 2017. Author(s), 2017. http://dx.doi.org/10.1063/1.5011882.

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Susilowati, Agustine, Yati Maryati, Hakiki Melanie, and Aspiyanto Aspiyanto. "Recovering cholesterol binder from inulin hydrolysate by Bifidobacterium bifidum as dietary fiber by means of dead-end stirred ultrafiltration cell (DESUFC)." In SolarPACES 2017: International Conference on Concentrating Solar Power and Chemical Energy Systems. Author(s), 2018. http://dx.doi.org/10.1063/1.5064333.

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Sharifullina, D. T., R. N. Nizamov, R. N. Nizamov, I. R. Yunusov, and G. I. Rakhmatullina. "STUDYING THE POSSIBILITY OF JOINT CULTIVATION OF B.BIFIDUM AND E.COLI ON ADAPTED NUTRIENT MEDIA." In STATE AND DEVELOPMENT PROSPECTS OF AGRIBUSINESS Volume 2. DSTU-Print, 2020. http://dx.doi.org/10.23947/interagro.2020.2.423-426.

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Microbial substances introduced into the body of animals increase radio stability and reduce the mortality rate. The greatest significance can be obtained by using vaccines based on bacteria of the intestinal-typhoid group, which in the process of life produce antibacterial substances, enzymes, antigens, entero-and exotoxins, and cytokines with radioprotective properties. The tests revealed a complex mechanism of interaction between bifidobacteria and Escherichia in their joint cultivation. The biomass accumulation of E.coli strain «PL-6» and B.bifidum 1 during co-cultivation depended on the ratio of live bacteria E.coli strain «PL-6» and B.bifidum 1. Microcopy of smears made on days 1-4 from monocultures showed that the grown microbes in morphology corresponded to these cultures. The concentration of microorganisms, determined by tenfold dilution by the above method, was 1x109 CFU/ml - E.coli and 1x107 CFU/ml B.bifidum, with a sowing dose of each type of microbe 1x108 CFU/ml. Microcopy of smears made from a mixture of cultures showed that a dilution of 0,9:1,1-1,0:1,0 is most optimal for co-growing bifidum and Escherichia coli, since with a relatively equal number of monocultures on the 1st day Escherichiae multiply intensely, splitting the components of the Blaurock medium and inhibiting the growth of bifidum, but from the 3rd day B.bifidum begins to prevail, splitting E.coli and assimilating substances cleaved by E.coli.
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Reports on the topic "Bifidobacterium bifidum"

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Irkin, Reyhan. The Survival of Bifidobacterium bifidum NRRL B41410 in Traditional Beverage: Shalgam. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, September 2018. http://dx.doi.org/10.7546/crabs.2018.09.18.

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Stifeev, A. I., V. I. Lazarev, and O. V. Nikitina. NEW APPROACHES TO THE DEVELOPMENT OF A BIOLOGICALLY ACTIVE SUPPLEMENT BASED ON BIFIDOBACTERIUM BIFIDUM METABOLITES. FGBOU VO Kursk State Agricultural Academy, Journal Bulletin of the Kursk State Agricultural Academy., 2020. http://dx.doi.org/10.18411/issn1997-0749.2020-05-18.

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