Academic literature on the topic 'Biflavonoids'
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Journal articles on the topic "Biflavonoids"
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Windsor, Peter K., Stephen P. Plassmeyer, Dominic S. Mattock, Jonathan C. Bradfield, Erika Y. Choi, Bill R. Miller, and Byung Hee Han. "Biflavonoid-Induced Disruption of Hydrogen Bonds Leads to Amyloid-β Disaggregation." International Journal of Molecular Sciences 22, no. 6 (March 12, 2021): 2888. http://dx.doi.org/10.3390/ijms22062888.
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Deposition of amyloid β (Aβ) fibrils in the brain is a key pathologic hallmark of Alzheimer’s disease. A class of polyphenolic biflavonoids is known to have anti-amyloidogenic effects by inhibiting aggregation of Aβ and promoting disaggregation of Aβ fibrils. In the present study, we further sought to investigate the structural basis of the Aβ disaggregating activity of biflavonoids and their interactions at the atomic level. A thioflavin T (ThT) fluorescence assay revealed that amentoflavone-type biflavonoids promote disaggregation of Aβ fibrils with varying potency due to specific structural differences. The computational analysis herein provides the first atomistic details for the mechanism of Aβ disaggregation by biflavonoids. Molecular docking analysis showed that biflavonoids preferentially bind to the aromatic-rich, partially ordered N-termini of Aβ fibril via the π–π interactions. Moreover, docking scores correlate well with the ThT EC50 values. Molecular dynamic simulations revealed that biflavonoids decrease the content of β-sheet in Aβ fibril in a structure-dependent manner. Hydrogen bond analysis further supported that the substitution of hydroxyl groups capable of hydrogen bond formation at two positions on the biflavonoid scaffold leads to significantly disaggregation of Aβ fibrils. Taken together, our data indicate that biflavonoids promote disaggregation of Aβ fibrils due to their ability to disrupt the fibril structure, suggesting biflavonoids as a lead class of compounds to develop a therapeutic agent for Alzheimer’s disease.
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Girardi, Leury G. J., Michele Morsch, Ana E. Oliveira, Valdir Cechinel-Filho, and Clóvis Antonio Rodrigues. "Separation of Bioactive Biflavonoids from Rheedia gardneriana Using Chitosan Modified with Benzaldehyde." Zeitschrift für Naturforschung C 60, no. 5-6 (June 1, 2005): 408–10. http://dx.doi.org/10.1515/znc-2005-5-607.
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This paper shows the influence of benzenic groups on the chitosan surface for the separation of bioactive biflavonoids from Rheedia gardneriana leaves. The yield of the biflavonoids using chitin modified with benzaldehyde (CH-Bz) as adsorbent in column chromatography was higher than that achieved with silica gel and chitosan. The presence of benzenic groups decreases the polarity of chitosan and consequently the interaction of hydrogen bonding between phenolic hydroxyl (OH) of biflavonoids and amine groups of the adsorbent. Therefore, the separation of these compounds appears to be the result of hydrophobicity and π-π interaction among electrons from the aromatic ring in sorbent and biflavonoid molecules.
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Al-Shagdari, Ahmed, Adonis Bello Alarcón, Osmany Cuesta-Rubio, Anna Lisa Piccinelli, and Luca Rastrelli. "Biflavonoids, Main Constituents from Garcinia Bakeriana Leaves." Natural Product Communications 8, no. 9 (September 2013): 1934578X1300800. http://dx.doi.org/10.1177/1934578x1300800913.
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The genus Garcinia is a source of a large variety of organic compounds including biflavonoids, acylphloroglucinols and xanthones mainly, but few data are available about the chemical composition of Cuban species. The aim of this investigation was to identify the main constituents of G. bakeriana Urb., a rare Cuban endemic plant. A new biflavonoid, 4″’- O-methyl-I3,II8-biapigenin (1), together with 9 known compounds, namely, the biflavonoids amentoflavone (2), 4″’- O-methylamentoflavone (3), 4′- O-methylcupressuflavone (4), GB-2a (5), volkensiflavone (6), 6″-(2-hydroxy-3-methyl-3-butenyl)-amentoflavone (7), I3,II8-biapigenin (8), and GB-1a (9), and the xanthone norathyriol (10), were isolated from the leaves of this species. All the structures were elucidated by spectroscopic methods including 1D and 2D NMR experiments, as well as ESIMS analysis. These results showed that the isolated biflavonoids possess a C-C interflavonoid linkage between the apigenin units or its derivatives.
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Li, Li, Xia, Tian, Zhang, Rui, Dong, and Xiao. "Anticancer Effects of Five Biflavonoids from Ginkgo Biloba L. Male Flowers In Vitro." Molecules 24, no. 8 (April 16, 2019): 1496. http://dx.doi.org/10.3390/molecules24081496.
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Ginkgo biloba L., an ancient dioecious gymnosperm, is now cultivated worldwide for landscaping and medical purposes. A novel biflavonoid—amentoflavone 7''-O-β-D-glucopyranoside (1)—and four known biflavonoids were isolated and identified from the male flowers of Ginkgo. The anti-proliferative activities of five biflavonoids were evaluated on different cancer lines. Bilobetin (3) and isoginkgetin (4) exhibited better anti-proliferative activities on different cancer lines. Their effects were found to be cell-specific and in a dose and time dependent manner for the most sensitive HeLa cells. The significant morphological changes validated their anticancer effects in a dose-dependent manner. They were capable of arresting the G2/M phase of the cell cycle, inducing the apoptosis of HeLa cells dose-dependently and activating the proapoptotic protein Bax and the executor caspase-3. Bilobetin (3) could also inhibit the antiapoptotic protein Bcl-2. These might be the mechanism underlying their anti-proliferation. In short, bilobetin (3) and isoginkgetin (4) might be the early lead compounds for new anticancer agents.
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Skopp, Gisela, and Gerhard Schwenker. "Biflavonoide aus Schinus terebinthifolius Raddi (Anacardiaceae)/ Biflavonoids from Schinus terebinthifolius Raddi (Anacardiaceae)." Zeitschrift für Naturforschung B 41, no. 11 (November 1, 1986): 1479–82. http://dx.doi.org/10.1515/znb-1986-1125.
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Cao, Shu-Geng, Keng-Yeow Sim, and Swee-Hock Goh. "Biflavonoids ofCalophyllum venulosum." Journal of Natural Products 60, no. 12 (December 1997): 1245–50. http://dx.doi.org/10.1021/np970303f.
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Tih, Anastasie Ewola, Raphael Tih Ghogomu, Beibam Lucas Sondengam, Christelle Caux, and Bernard Bodo. "Minor Biflavonoids fromLophiraalataLeaves." Journal of Natural Products 69, no. 8 (August 2006): 1206–8. http://dx.doi.org/10.1021/np050169w.
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Recalde-Gil, Angelica Maria, Luiz Klein-Júnior, Juliana Salton, Sérgio Bordignon, Valdir Cechinel-Filho, Cristiane Matté, and Amélia Henriques. "Aromatase (CYP19) inhibition by biflavonoids obtained from the branches of Garcinia gardneriana (Clusiaceae)." Zeitschrift für Naturforschung C 74, no. 9-10 (September 25, 2019): 279–82. http://dx.doi.org/10.1515/znc-2019-0036.
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Abstract Overexpression of aromatase in breast cancer cells may substantially influence its progression and maintenance. In this sense, the inhibition of aromatase is a key target for the treatment of breast cancer in postmenopausal women. Although several flavonoids had already demonstrated the capacity of inhibiting aromatase activity, the role of biflavonoids as aromatase inhibitors is poorly studied. In this work, the biflavonoids isolated from Garcinia gardneriana, morelloflavone (1), Gb-2a (2) and Gb-2a-7-O-glucose (3) were submitted to in vitro assay to evaluate the aromatase modulatory effect. As results, it was demonstrated that all biflavonoids were able to inhibit the enzyme, with IC50 values ranging from 1.35 to 7.67 μM. This demonstrates that biflavonoids are an important source of scaffolds for the development of new aromatase inhibitors, focusing on the development of new anticancer agents.
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Yokosuka, Akihito, Misaki Honda, Hitoshi Kondo, and Yoshihiro Mimaki. "Chemical Constituents of the Whole Plant of Verbena hastata and Their Inhibitory Activity Against the Production of AGEs." Natural Product Communications 16, no. 4 (April 2021): 1934578X2110097. http://dx.doi.org/10.1177/1934578x211009727.
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Two iridoid glycosides (1 and 2), 3 phenolic glycosides (3–5), 1 flavone glycoside (6), 3 biflavonoids (7–9), 1 flavone (10), 2 triterpenes (11 and 12), 1 sterol (13), and 1 naphthoquinone derivative (14) were isolated from the whole plant of Verbena hastata (Verbenaceae). Compounds 3-13 were isolated from V. hastata for the first time. Compound 14 is undescribed in the literature. Incubation of glyceraldehyde and collagen either with phenolic glycosides (3), (4), or (5) or with biflavonoid (8) inhibited the production of advanced glycation end products, with IC50 values of 6.3, 6.4, 6.2, and 6.8 mM, respectively. Aminoguanidine, which was used as a positive control, had an IC50 value of 10.2 mM.
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Zhang, Xiaoli, Guocai Wang, Weihuan Huang, Wencai Ye, and Yaolan Li. "Biflavonoids from the Roots of Wikstroemia indica." Natural Product Communications 6, no. 8 (August 2011): 1934578X1100600. http://dx.doi.org/10.1177/1934578x1100600815.
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Two new biflavonoids, 4′-methoxydaphnodorin D1 and 4′-methoxydaphnodorin D2, along with six known biflavonoids, were isolated from the roots of Wikstroemia indica. The structures of the new compounds were determined by extensive NMR and HRESIMS spectroscopic analyses in combination with CD measurements.
Dissertations / Theses on the topic "Biflavonoids"
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Recalde, Gil Maria Angélica. "Potencial inibitório in vitro de biflavonoides de Garcinia gardneriana : um estudo sobre monoamina oxidades e CYP19 (aromatase)." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2015. http://hdl.handle.net/10183/149485.
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The plant Garcinia gardneriana (Planch. & Triana) Zappi, popularly known in Brazil as "bacupari" has traditionally been used for various types of inflammatory diseases and the evaluation of their chemical composition, mainly of leaves, has resulted in biflavonoids as major compounds. These phenolic compounds have shown anti-inflammatory activity validating the popular use of the plant. In this work was isolated from dried branches of Garcinia gardneriana the biflavonoids: morelloflavone, that is an naringenin covalently linked to luteolin, Gb-2a which is an naringenin linked to eriodictyol and Gb-2a- 7-O-glucose. These compounds have been previously evaluated in various activities such as anti-inflammatory and anti-antioxidants but there is no report of its activity as enzymatic inhibitors. However, the monomers that form it, have been evaluated in the inhibition of aromatase and antidepressant activity with positive outcome, which commonly are used MAO-A inhibitors. In the isolation process were also founded terpenoid compounds as lupeol and friedelin The isolated and purified biflavonoids were used to evaluate enzyme inhibition "in vitro" in monoamine oxidases (MAO-A MAO-B) and aromatase. The compounds showed a positive response even of IC50 5,47 μM and 1,35 μM for MAO-A inhibition of and aromatase enzyme respectively; discovering a way for a new proposal to link both enzymes for treatment of hormone-dependent cancers and anxiety and depression disorders.La planta Garcinia gardneriana (Planch. & Triana) Zappi, popularmente conocida en Brasil como "bacupari" ha sido tradicionalmente usada para varios tipos de enfermedades inflamatorias y la evaluación de su composición química, principalmente de las hojas, ha resultado en biflavonoides como compuestos mayoritarios. Estos compuestos fenólicos han demostrado actividad anti-inflamatória validando el uso popular de la planta. En este trabajo se asilaron a partir de tallos secos de la Garcinia gardneriana los biflavonoides: moreloflavona, que consiste en una naringenina unida covalentemente a luteolina, Gb-2a que es un compuesto que consiste en una naringenina unida a un eriodictyol y Gb-2a-7-O-glucose. Estos compuestos ya han sido previamente evaluados en diversas actividades como anti inflamatorios y anti antioxidantes pero no se tiene reporte de su actividad como inhibidores enzimáticos. Sin embargo, los monomeros que los conforman han sido evaluados en la inhibición de la aromatasa y con resultados positivos como en la actividad antidepresiva, para la cual comúnmente son usados los inibidores de MAO-A. En el proceso de aislamiento también fueron encontrados compuestos terpenoides como lupeol y friedelina. Los biflavonoides aislados y purificados se usaron para evaluar la inhibición enzimática “in vitro” en monoaminooxidasas (MAO-A, MAO-B) y aromatasa. Los compuestos presentaron una respuesta positiva calculada con IC50 de hasta 5,47 μM y 1,35 μM para la inhibición de las enzimas MAO-A y aromatasa respectivamente, abriendo el camino a una nueva propuesta de relacionar estas dos enzimas para tratamiento de cánceres hormonodependientes y transtornos de ansiedad y depresión.
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Oliveira, José Cândido Selva de. "Isolamento de constituintes e síntese de flavonoides encontrados em Poincianella pyramidalis (Fabaceae) e análise fitoquímica de Theobroma cacao (Malvaceae)." Instituto de Química, 2014. http://repositorio.ufba.br/ri/handle/ri/16127.
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CNPq e CAPES
Os extratos MeOH de Poincinella pyramidalis (caatingueira), obtidas da casca da raiz (PPCR) e das flores (PPF), bem como, as frações orgânicas obtidas por partição, foram submetidas a testes de atividade citotóxica, antioxidante e anticolinesterásica. O teste citotóxico contra Artemia salina revelou atividade moderada para fase AcOEt em PPCR. Assim como, a fase AcOEt de PPCR apresentou 75% de inibição no teste anticolinesterásico. Para a atividade antioxidante, as fase AcOEt e BuOH de PPF foi a mais ativa, sendo atribuída a presença de galato de metila (PPF3). Por meio de processos cromatográficos foi possível isolar da fase DCM de PPF as substâncias PPF1 (alcoóis graxos), PPF2a-b (β–sitosterol/estigmasterol), PPF3 (galato de metila) e PPF4 (α e β–amirina). Além dessas, foram isoladas da fase hexano de PPCR ésteres metílicos (PPCR1) e o biflavonoide 5,7-dihidroxi-4´-metoxiflavona-6α-2´´´,4´´´-dihidroxi-4´´- metoxidihidrochalcona (PPCR3). Da fase hidroMeOH de PPCR foram isoladas as substâncias PPCR2 (ácido 3,3´-dimetoxi-4-hidroxielagico-4´-β-D-xilopiranosideo) e outro biflavonoide PPCR4 (5-hidroxi-7,4´-dimetoxiflavona-3α-2´´´-hidroxi-4´´´,4´´- dimetoxidihidrochalcona). Sendo PPCR3 e PPCR4 inéditas na literatura. O extrato hexano de Theobroma cacao (cacau) obtido das sementes (STC) foi analisado por RMN e revelou presença de triacilglicerideos. A otimização e fracionamento do extrato MeOH de T. cacao utilizando CCC levou ao isolamento simultâneo de teobromina (2), teofilina (3) e cafeína (4), fase móvel hexano:AcOEt (2:3) e estacionária MeOH:H2O (1:1). Um método analítico foi desenvolvido empregando HPLC/DAD com coluna fase reversa C-18 para a quantificação de 2, 3 e 4 nos extrativos em fase ácida/básica e nas frações STC6-22 e STC23-67 obtidas por CCC. Assim, os teores de 2 e 4 para no extrato MeOH de T. cacao e frações obtidas por CCC foram determinados. A teofilina (3) detectada e quantificada na fração STC6-22 em baixa concentração (0,78 mg/g) em relação a 2 e 4. A apigenina (6) foi sintetizada utilizando dois métodos distintos (“A” e “B”). O metodo “B” apresentou rendimento de 74% em uma única etapa. A síntese de derivados O-metilados forneceu bons rendimentos (> 90%). As substâncias isoladas/sintetizadas foram submetidas à atividade anticolinesterasica do qual, são inativas. As mesmas foram elucidadas a partir de dados de IV, UV, EM, α[D], RMN de 1H e 13C (BB e APT), RMN de correlação (HMQC, HSQC e HMBC).
The MeOH extracts from Poincinella pyramidalis (caatingueira), obtained of bark root (PPCR) and flowers (PPF), as well the organic fractions obtained by partition, have been tested for cytotoxicity, antioxidant and anticholinesterasic activity. The cytotoxic test against Artemia salina showed moderate activity for AcOEt phase in PPCR. Well the EtOAc phase of PPCR showed 75% inhibition on anticholinesterasic test. For the antioxidant activity, the EtOAc and BuOH phase of PPF was the most active, being attributed to the presence of methyl gallate (PPF3). For chromatographic process was possible to isolate the DCM phase of PPF the substances PPF1 (fatty alcohols), PPF2a- b (β–sitosterol/stigmasterol), PPF3 (methyl galate) and PPF4 (α- and β–amirin). Besides these, were isolated from the hexane phase PPCR methyl esters (PPCR1) and the biflavonoid 5,7-dihidroxy-4´-methoxyflavone-6α-2´´´,4´´´-dihidroxy-4´´-methoxy dihidrochalcone (PPCR3). The HidroMeOH phase of PPCR were the isolated substances PPCR2 (3,3´-dimethoxi-4-hidroxyellagicacid-4´-β-D-xylopiranoside) and other biflavonoid PPCR4 (5-hidroxy-7,4´-dimethoxyflavone-3α-2´´´-hidroxy-4´´´,4´´- dimethoxydihidrochalcone). Being PPCR3 and PPCR4 unpublished in literature. Hexane extract of Theobroma cacao (cacau) obtained from the seeds (STC) was analyzed by NMR and revealed the presence of triacylglycerides. The optimization and fractionation of MeOH extract of T. cacao using CCC led to the simultaneous isolation of theobromine (2) Theophylline (3) and caffeine (4), mobile phase hexane:EtOAc (2:3) and stationary MeOH:H2O (1:1). An analytical method was developed employing HPLC/DAD with reverse phase column C-18 for the measurement of 2, 3 and 4 in the extractives acid/base phase and the STC6-22 and STC23-67 fractions obtained by CCC. So, the levels of 2 and 4 for the MeOH extract of T. cacao and fractions obtained by CCC were determined. Theophylline (3) detected and quantified in the fraction STC6-22 low concentration (0.78 mg/g) in relation the 2 and 4. Apigenin (6) was synthesized using two different methods ("A" and "B"). The method "B" had a yield of 74% in one step. The synthesis of O-methylated derivatives provided good yields (> 90%). The isolated/synthesized substances were submitted to anticholinesterasic activity which are inactive. The same were elucidated from data of IR, UV, MS, α[D], 1H NMR and 13C NMR (BB and APT) NMR correlation (HMQC, HSQC and HMBC).
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Santos, Elis?ngela. "Identifica??o de inibidores oriundos de fontes naturais para o modelo do receptor atpase-ca+2 do Plasmodium falciparum (PfATP6)." Universidade Estadual de Feira de Santana, 2014. http://localhost:8080/tede/handle/tede/105.
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Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES
Malaria is considered a non-contagious chronic evolution with episodic manifestations of acute character infectious disease, which afflicts millions of people in tropical and subtropical areas of the world. Vegetables represent a source of active molecules for various diseases, including malaria. Through phytochemical procedures, one can study the secondary metabolism of these plants where they hope to find new sources of treatment for malaria. The present study had as main objective to identify molecules derived from natural sources can inhibit the ATPase-Ca 2 + protein of Plasmodium falciparum (PfATP6). The molecular target was the model PfATP6 receiver, obtained by comparative modeling. Was performed using the program AutoDock Vina 1.1.2. screening in databases with the aim of selecting the 10 most promising ligands, with determination of the energies of affinity. Subsequently, tests with hydromethanolic and chloroform extracts of the species of Tul Cenostigma macrophyllum were performed. The patterns of agatisflavona and amentoflavone biflavonoids were analyzed by High Performance Liquid Chromatography for Detection Arrangement Diodes (HPLC-DAD) to identify the presence of these metabolites in the species. Validation of the chromatographic method took into account the parameters selectivity, linearity, precision, accuracy, limit of detection and limit of quantification. The results showed molecular next coupling energy variation among the 10 selected compounds (1 kcal / mol). With the results of the molecular coupling of the intermolecular interactions were studied and agatisflavona amentoflavone with PfATP6 receiver comparing them with the values obtained for thapsigargin. Since the energy values affinity the compounds amentoflavone -10.0 kcal / mol and agatisflavona -9.7 kcal / mol ortost?rico showed affinity for the receptor site of PfATP6, these interactions being supported by the nature of intermolecular interactions identified. By comparing the retention times and ultraviolet spectra (UV) of the standards and samples could be identified agatisflavona amentoflavone and biflavonoids the leaves and stem bark of C. macrophyllum. Quantification of agatisflavona biflavonoide ranged from 22.25 to 0.012 mg of agatisflavona / g dry plant and amentoflavone ranged from 20.63 to 0.02 mg of amentoflavone / g dry plant. The analysis of the parameters evaluated showed that the method was suitable for the analysis of agatisflavona and amentoflavone, performing in line with the specifications of the current legislation. It is noticed that the amentoflavone agatisflavona compounds and can act in PfATP6 receiver, directing further studies of interactions in biological target in perspective for malaria control. Thus, it is possible to suggest that there are natural compounds present in semiarid with possible antimalarial activity, wherein the molecular modeling tool associated with the phytochemical analysis can guide the identification of bioactive compounds, and therefore the development of new drugs.
A mal?ria ? considerada como uma doen?a infecciosa, n?o contagiosa de evolu??o cr?nica com manifesta??es epis?dicas de car?ter agudo, a qual aflige milh?es de pessoas nas zonas tropicais e subtropicais do globo. Os vegetais representam fonte de mol?culas ativas para diversas enfermidades, incluindo a mal?ria. Atrav?s de procedimentos fitoqu?micos, pode-se estudar o metabolismo secund?rio desses vegetais onde se esperam encontrar novas fontes de tratamento para a mal?ria. O presente estudo teve como objetivo geral identificar mol?culas oriundas de fontes naturais capazes de inibir a prote?na ATPase-Ca+2 do Plasmodium falciparum (PfATP6). O alvo molecular foi o modelo do receptor PfATP6, obtido por modelagem comparativa. Foi realizado atrav?s do programa AutoDock Vina 1.1.2. uma triagem em bancos de dados com o objetivo de selecionar os 10 ligantes mais promissores, com determina??o das energias de afinidade. Posteriormente, foram realizados testes com os extratos hidrometan?lico e clorof?rmico da esp?cie de Cenostigma macrophyllum Tul. Os padr?es dos biflavonoides agatisflavona e amentoflavona foram analisados por Cromatografia L?quida de Alta Efici?ncia por Detec??o de Arranjo de Diodos (CLAE-DAD) para identifica??o da presen?a destes metab?litos na esp?cie. A valida??o do m?todo cromatogr?fico levou em considera??o os par?metros seletividade, linearidade, precis?o, exatid?o, limite de detec??o e limite de quantifica??o. Os resultados de acoplamento molecular apresentaram varia??o energ?tica pr?xima entre os 10 compostos selecionados (1 kcal/mol). De posse dos resultados do acoplamento molecular foram estudadas as intera??es intermoleculares da agatisflavona e amentoflavona com o receptor PfATP6 comparando-os com os valores obtidos para a tapsigargina. Dado os valores de energia de afinidade, os compostos amentoflavona -10,0 Kcal/mol e agatisflavona -9,7 Kcal/mol apresentaram afinidade com o s?tio ortost?rico do receptor PfATP6, sendo essas intera??es sustentadas pela natureza das intera??es intermoleculares identificadas. Atrav?s da compara??o dos tempos de reten??o e espectros de Ultra Violeta (UV) dos padr?es e amostras foi poss?vel identificar os biflavonoides agatisflavona e amentoflavona nas folhas e casca do caule de C. macrophyllum. A quantifica??o do biflavonoide agatisflavona variou de 22,25 a 0,012 ?g de agatisflavona/g de planta seca e da amentoflavona variou de 20,63 a 0,02 ?g de amentoflavona/g de planta seca. A an?lise dos par?metros avaliados demonstrou que o m?todo foi adequado para a an?lise da agatisflavona e amentoflavona, apresentando-se em conson?ncia com as especifica??es da legisla??o vigente. Percebe-se que os compostos amentoflavona e agatisflavona podem atuar no receptor PfATP6, direcionando novos estudos de intera??es nesse alvo biol?gico na perspectiva de controle da mal?ria. Desta forma ? poss?vel sugerir que existem compostos naturais presente no semi?rido com poss?vel atividade antimal?rica, no qual a ferramenta da modelagem molecular associada ?s analises fitoqu?micas pode guiar na identifica??o de compostos bioativos e, por conseguinte no desenvolvimento de novos f?rmacos.
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Burgos, Rosilene Cristina Rossetto. "Detecção in silico, isolamento e caracterização estrutural dos constitutintes micromoleculares antimaláricos e antioxidantes em galhosmdemGarcina gardneriana (Clusiaceae) /." Araraquara, [s.n.], 2010. http://hdl.handle.net/11449/97014.
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Resumo: Este trabalho teve como principais objetivos a detecção, isolamento e elucidação estrutural dos constituintes micromoleculares presentes em folhas e galhos de Garcinia gardneriana, uma espécie da família Clusiaceae com poucos relatos na literatura sobre a sua composição fitoquímica. A detecção das moléculas foi realizada através de abordagens in silico com o estabelecimento de perfis cromatográficos e espectrométricos dos constituintes majoritários dos extratos brutos e frações. A comparação desses dados foi realizada através de dados existentes na literatura. A partir dessa metodologia foi possível detectar 11 triterpenos por GC-FID nos extratos e frações apolares: campesterol (1), estigmasterol (2), β-sitosterol (3), β- amirenona (4), α- amirina (5), β-amirina (6), lupenona (7), lupeol (8), acetato de β-amirina (9), acetato de α-amirina (10), friedelina (11) e mais 9 biflavonóides nos extratos e frações mais polares: xantochimusídeo (12), GB2 (13), fukugisídeo (14), GB 1a glicosilado (15), GB2a (16), morelloflavona (17), volkensiflavona (18), amentoflavona (19), podocarpusflavona (20) e uma benzofenona: 7-epiclusianona (21) através da técnica HPLC-DAD-ESI-MSHRMS. Quanto aos bioensaios in vitro realizados (inibição da polimerização do heme, redução do radical DPPH (antioxidante), inibidor da enzima acetilcolinesterase, tripanocida, citotóxico, antifúngico contra fitopatógenos e patógenos humanos) em todos os extratos e frações preparados, alguns resultados merecem destaque tais como a redução do radical DPPH com IC50 de 9,5, 7,4 e 7,5 μg/mL respectivamente para o extrato etanólico dos galhos, a fração acetato de etila dos galhos e folhas, a inibição da polimerização do heme com valores de 95,7, 98,6 e 68,0 %, de inibição para o extrato etanólico das folhas, e frações acetato de etila das folhas e galhos respectivamenteAbstract: This work had as main objective the detection, isolation and structural elucidation of the micromolecular constituents from leaves and twigs of Garcinia gardneriana, a Clusiaceae species with few literature reports regarding its phytochemical composition. The detection of molecules was performed by in silico detection with the establishment of chromatographic and spectroscopic profiles of the major constituents present in the crude extracts and fractions as well as comparison of data from database and literature reports. Based on this methodology we were able to detect 11 triterpenes by GC-FID in the extracts and nonpolar fractions: campesterol (1), stigmasterol (2), β-sitosterol (3), β-amirenone (4), α- amyrin (5), β-amyrin (6), lupenone (7), lupeol (8), β-amyrin acetate (9), α-amyrin acetate (10), friedelin (11) and more 9 biflavonoids from extracts and fractions polar: xanthochymuside (12) and GB2 (13), fukugiside (14), GB1 glycosylated (15), GB2a (16), morelloflavone (17), volkensiflavone (18), amentoflavone (19) , podocarpusflavone (20), 7-epiclusianone (21) by HPLC-DAD-ESI-MS-HRMS. As for the performed in vitro bioassays (inhibition of heme polymerization, reduction of the DPPH radical (antioxidant), inhibition of the enzyme acetylcholinesterase, trypanocidal, cytotoxic antifungal activity against plant pathogens and human pathogens) in all extracts and fractions prepared in this work, some results are worthy of mentioning such as the reduction of the DPPH radical with IC50 values of 9.5, 7.4 and 7.5 μg/mL respectively for the ethanol extract from twigs, the ethyl acetate fraction from the branches and leaves, as well as the inhibition of heme polymerization with values of 95.7, 98.6 and 68.0% from the ethanol extract from the leaves, and the ethyl acetate fractions leaves and branches
Orientador: Ian Castro Gamboa
Coorientador: Márcia Nasser Lopes
Banca: Mario Sergio Palma
Banca: Carlos Alexandre Carollo
Mestre
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Burgos, Rosilene Cristina Rossetto [UNESP]. "Detecção in silico, isolamento e caracterização estrutural dos constitutintes micromoleculares antimaláricos e antioxidantes em galhosmdemGarcina gardneriana (Clusiaceae)." Universidade Estadual Paulista (UNESP), 2010. http://hdl.handle.net/11449/97014.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Este trabalho teve como principais objetivos a detecção, isolamento e elucidação estrutural dos constituintes micromoleculares presentes em folhas e galhos de Garcinia gardneriana, uma espécie da família Clusiaceae com poucos relatos na literatura sobre a sua composição fitoquímica. A detecção das moléculas foi realizada através de abordagens in silico com o estabelecimento de perfis cromatográficos e espectrométricos dos constituintes majoritários dos extratos brutos e frações. A comparação desses dados foi realizada através de dados existentes na literatura. A partir dessa metodologia foi possível detectar 11 triterpenos por GC-FID nos extratos e frações apolares: campesterol (1), estigmasterol (2), β-sitosterol (3), β- amirenona (4), α- amirina (5), β-amirina (6), lupenona (7), lupeol (8), acetato de β-amirina (9), acetato de α-amirina (10), friedelina (11) e mais 9 biflavonóides nos extratos e frações mais polares: xantochimusídeo (12), GB2 (13), fukugisídeo (14), GB 1a glicosilado (15), GB2a (16), morelloflavona (17), volkensiflavona (18), amentoflavona (19), podocarpusflavona (20) e uma benzofenona: 7-epiclusianona (21) através da técnica HPLC-DAD-ESI-MSHRMS. Quanto aos bioensaios in vitro realizados (inibição da polimerização do heme, redução do radical DPPH (antioxidante), inibidor da enzima acetilcolinesterase, tripanocida, citotóxico, antifúngico contra fitopatógenos e patógenos humanos) em todos os extratos e frações preparados, alguns resultados merecem destaque tais como a redução do radical DPPH com IC50 de 9,5, 7,4 e 7,5 μg/mL respectivamente para o extrato etanólico dos galhos, a fração acetato de etila dos galhos e folhas, a inibição da polimerização do heme com valores de 95,7, 98,6 e 68,0 %, de inibição para o extrato etanólico das folhas, e frações acetato de etila das folhas e galhos respectivamente
This work had as main objective the detection, isolation and structural elucidation of the micromolecular constituents from leaves and twigs of Garcinia gardneriana, a Clusiaceae species with few literature reports regarding its phytochemical composition. The detection of molecules was performed by in silico detection with the establishment of chromatographic and spectroscopic profiles of the major constituents present in the crude extracts and fractions as well as comparison of data from database and literature reports. Based on this methodology we were able to detect 11 triterpenes by GC-FID in the extracts and nonpolar fractions: campesterol (1), stigmasterol (2), β-sitosterol (3), β-amirenone (4), α- amyrin (5), β-amyrin (6), lupenone (7), lupeol (8), β-amyrin acetate (9), α-amyrin acetate (10), friedelin (11) and more 9 biflavonoids from extracts and fractions polar: xanthochymuside (12) and GB2 (13), fukugiside (14), GB1 glycosylated (15), GB2a (16), morelloflavone (17), volkensiflavone (18), amentoflavone (19) , podocarpusflavone (20), 7-epiclusianone (21) by HPLC-DAD-ESI-MS-HRMS. As for the performed in vitro bioassays (inhibition of heme polymerization, reduction of the DPPH radical (antioxidant), inhibition of the enzyme acetylcholinesterase, trypanocidal, cytotoxic antifungal activity against plant pathogens and human pathogens) in all extracts and fractions prepared in this work, some results are worthy of mentioning such as the reduction of the DPPH radical with IC50 values of 9.5, 7.4 and 7.5 μg/mL respectively for the ethanol extract from twigs, the ethyl acetate fraction from the branches and leaves, as well as the inhibition of heme polymerization with values of 95.7, 98.6 and 68.0% from the ethanol extract from the leaves, and the ethyl acetate fractions leaves and branches
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FERREIRA, Rafaela Oliveira. "Contribuição ao estudo químico e avaliação da atividade antioxidante dos frutos verdes de Clusia paralicola (Clusiaceae)." Universidade Federal Rural de Pernambuco, 2011. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6373.
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Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq
Clusia paralicola (Clusiaceae) occurrs in forests from northeastern Brazil, especially in the semi-arid and wet forests, is popularly known as pororoca. This work describes the chemical and antioxidant activity of the green fruits from Clusia paralicola G. Mariz Cunha and resulted in the isolation and identification of two biflavonoids (GB1-7"-Oglucoside and 3,8 '-binaringenin-7"-O-β-glucoside), a catechin (epicatechin), two steroids (β-sitosterol and stigmasterol), a phenylpropanoid (1,3,5-trimethoxy-2- propenilbenzen) and a triterpene (β-amyrin). The structures of isolated compounds were elucidated by analysis of NMR spectra of ¹H and ¹³C, including 2D and LC-ESI-MS The absolute stereochemistry of biflavonoids was determined by circular dichroism analysis and complete structural elucidation was performed to GB1-7-O-glucoside. To determine the total phenolic content was used Folin-Ciocalteu test and to evaluate the antioxidant potential were used DPPH, ABTS and the system β-carotene/ linoleic acid with the EtOH extract and hexane, EtOAc and MeOH/H2O fractions. Evaluation of antioxidant activity was also performed with mixtures of two biflavonoids. For the total phenolic content, antiradicalar activity (DPPH and ABTS) and antioxidant activity with the system β carotene/linoleic acid were more active the EtOH extract and EtOAc fraction. The EtOH extract and fractions tested showed correlation with the total phenolic content and activity antioxidant. With the exception of β-amyrin, previously identified in the latex of C. paralicola, all substances are being reported for the first time in this species. The absolute stereochemistry was determined as (2R, 3S, 2”R, 3”R)-GB1-7-O-glucoside and (2R, 3S, 2”R)-3.8'-binaringenina-7-O-glycoside. These biflavonoids are inedited in the Clusia genus.
Clusia paralicola (Clusiaceae) têm ocorrência nas florestas nordestinas, especialmente do semi-árido e dos brejos de altitude, é conhecida popularmente como pororoca. Este trabalho descreve o estudo químico e a avaliação da atividade antioxidante dos frutos verdes de Clusia paralicola. O estudo químico resultou no isolamento e identificação de dois biflavonóides (GB-1-7”-O-glicosídeo e 3,8”-binaringenina-7”-O-β-glicosídeo), uma catequina (epicatequina), dois esteróides (β-sitosterol e estigmasterol), um fenilpropanóide (1,3,5-trimetoxi-2-propenilbenzeno) e um triterpeno (β-amirina). As estruturas das substâncias isoladas foram elucidadas pelas análises dos espectros de RMN de ¹H e ¹³C, incluindo 2D e LC-ESI-MS. A estereoquímica absoluta dos biflavonoides foi determinada através da análise de dicroismo circular e a elucidação estrutural completa foi realizada com GB1-7-O-glicosídeo. Para a determinação do teor de fenólicos totais foi utilizado o reagente de Folin-Ciocalteu e para avaliar o potencial antioxidante foram realizados os ensaios com DPPH, ABTS e o sistema β- caroteno/ácido linoléico com o extrato EtOHe frações hexânica, AcOEt e MeOH/H2O. A avaliação da atividade antioxidante foi realizada também com a mistura dos biflavonoides. Para o teor de fenólicos totais, atividade antiradicalar (ABTS e DPPH) e atividade antioxidante com o sistema β-caroteno/ácido linoléico foram mais ativos o extrato EtOH e a fração AcOEt. O extrato EtOH e frações testadas apresentaram correlação com o teor de fenólicos totais e atividade antioxidante. Com exceção da β- amirina, previamente identificada no látex de C. paralicola, todas as substâncias estão sendo relatadas pela primeira vez nesta espécie. A esteroquímica absoluta foi determinada como sendo (2R, 3S, 2”R, 3”R)- GB1-7-O-glicosídeo e (2R, 3S, 2”R)-3,8”- binaringenina-7-O-glicosídeo. Estes biflavonóides são inéditos no gênero Clusia.
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Santos, Calila Teixeira. "Frutos de Schinus terebinthifolius Raddi: extra??o ativa, estudo fitoqu?mico e incorpora??o em filmes bioativos de pectina." Universidade Estadual de Feira de Santana, 2017. http://localhost:8080/tede/handle/tede/595.
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Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES
The present study had as main objective to obtain bioactive extracts of the fruits of Schinus terebinthifolius Raddi (STF), with antioxidant and antimicrobial action, for incorporation in a bioactive package for foods, besides inhibition of acetylcholinesterase. The extraction process performed by focussed microwave (EAMF) and ultrasound (UAE). The comparison between extraction methods was monitored by the biological activities of these extracts. The results showed that for the yield there was no significant difference. For the EAMF assays, the best value of phenolic (5950 ? 1.4 mgGAE / g) and total flavonoids (1750 ? 3.2 mgEQ / g) was at the extraction time of 25 minutes at 51 ? C and with 75% ethanol. For the assays obtained by EAU, the best total phenolic content was (92.2 ? 0.8 mgGAE / g) in the EAU 10 test (extraction time of 30 minutes at 42 ? C and 50% ethanol) And flavonoids (67.72 ? 1.0 mgEQ / g) in the UAE 12 assay (extraction time of 17.5 minutes at 60 ? C and 50% ethanol). The best values for percent inhibition (% SRL) were 87.3% (EAMF 8) and 31.3% (UAE 12). Mass spectrometry analysis showed the presence of phenolic compounds and terpenes. In relation to the antimicrobial activity of extracts obtained by EAMF, the MIC for S. aureus was 0.5 mg / ml for E. coli 0.25 mg / ml, whereas the extract of EAU presented MIC of 0.5 mg / Ml for the two microorganisms analyzed. In the phytochemical study, it was possible to isolate 7 substances: agasthiflavone, tetrahydrorobustaflavone, masticadienoic acid (Z), masticadienoic acid (E), schinol, gallic acid and squalene, as well as a mixture with unsaturated fatty acid profile and a mixture with trisaccharide characteristics. Their chemical structures being determined by spectrophotometric methods such as 1H and 13C NMR, UV and MS. Two of the 17 EAMF extracts were selected, which were incorporated into an active high-methoxylation pectin-based film called "Extract 2" and "Extract 8" in various concentrations (0, 1, 3 and 5%). The films had a mean thickness of 0.007 mm for all concentrations of the extracts tested. It was observed in relation to the mechanical properties that there was an interaction of the polyphenolic components of the extracts with the materials used in the production of the films. The films were more permeable to water vapor (6.18-7.80 g.mm/m2.h.kPa) than the control film (5.12 g.mm/m2.h.kPa). The films of both extracts showed good thermal stability independent of the concentration tested. The films produced in this study presented antioxidant activity, and the films added from extract 8 presented the best% SRL values (54.45 and 67.08, respectively). All films obtained inhibition halo against the microorganisms tested. In view of the results, it can be inferred that FTS have antioxidant potential, antimicrobial activity and high inhibition of acetylcholinesterase and can be used in cosmetics, nutraceuticals and food industry, as well as a promising alternative in the production of active films.
O presente estudo teve como objetivo principal obter extratos bioativos dos frutos de Schinus terebinthifolius Raddi (STF), com a??o antioxidante e antimicrobiana, para incorpora??o em uma embalagem bioativa para alimentos, al?m de inibi??o da acetilcolinesterase. O processo de extra??o foi realizado por micro-ondas focalizado (EAMF) e ultrassom (EAU). A compara??o entre os m?todos de extra??o foi monitorada pelas atividades biol?gicas desses extratos. Os resultados mostraram que para o rendimento n?o teve diferen?a significativa. Para os ensaios de EAMF o melhor valor de fen?lico (5950 ? 1,4 mgGAE/g) e flavonoides totais (1750 ? 3,2 mgEQ/g) foi no tempo de extra??o de 25 minutos, na temperatura de 51 ?C e com 75% de etanol. Para os ensaios obtidos por EAU o melhor teor de fen?licos totais foi (92,2 ? 0,8 mgGAE /g) no ensaio EAU 10 (tempo de extra??o de 30 minutos, na temperatura de 42 ?C e com 50% de etanol) e de flavonoides (67,72 ? 1,0 mgEQ /g) no ensaio EAU 12(tempo de extra??o de 17,5 minutos, na temperatura de 60 ?C e com 50% de etanol). Os melhores valores para a percentagem de inibi??o (%SRL) foi de 87,3% (EAMF 8) e 31,3% (EAU 12). A an?lise por espectrometria de massa mostrou a presen?a de compostos fen?licos e terpenos. Em rela??o ? atividade antimicrobiana dos extratos obtidos por EAMF, a CIM para S. aureus foi de 0,5 mg/ml, para E. coli 0,25 mg/ml, enquanto que o extrato de EAU apresentou CIM de 0,5 mg/ml para os dois microrganismos analisados. No estudo fitoqu?mico foi poss?vel isolar 7 subst?ncias: agasthiflavona, tetrahidrorobustaflavona, ?cido masticadienoico (Z), ?cido masticadienoico (E), schinol, ?cido g?lico e o esqualeno, al?m de uma mistura com perfil de ?cidos graxos insaturados e uma mistura com caracter?stica de trissacar?deos. Sendo suas estruturas qu?micas determinadas por m?todos espectrom?tricos tais como RMN 1H e 13C, UV e EM. Selecionou-se 2 dos 17 extratos EAMF, os mesmos foram incorporados em um filme ativo, ? base de pectina de alta metoxila??o, denominados ?Extrato 2? e ?Extrato 8? em concentra??es diversas (0, 1, 3 e 5%). Os filmes apresentaram espessura m?dias de 0,007 mm para todas as concentra??es dos extratos testados. Observou-se em rela??o as propriedades mec?nicas que houve uma intera??o dos componentes polifen?licos dos extratos com os materiais utilizados na produ??o dos filmes. Os filmes foram mais perme?veis ao vapor d??gua (6,18?7,80 g.mm/m2.h.kPa) do que o filme controle (5,12 g.mm/m2.h.kPa). Os filmes de ambos extratos mostraram uma boa estabilidade t?rmica independente da concentra??o testada. Os filmes produzidos neste estudo apresentaram atividade antioxidante, sendo que os filmes adicionados do extrato 8 apresentaram os melhores valores de %SRL (54,45 e 67,08, respectivamente). Todos os filmes obtiveram halo de inibi??o contra os micro-organismo testados. Diante dos resultados encontrados pode-se inferir que os STF t?m potencial antioxidante, atividade antimicrobiana e alta inibi??o de acetilcolinesterase podendo ser utilizados em cosm?ticos, produtos nutrac?uticos e na ind?stria alimentar, al?m de uma alternativa promissora na produ??o de filmes ativos.
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Oliveira, José Cândido Selva de. "Estudo químico e avaliação biológica do extrato das cascas das raízes de Caesalpinia pyramidalis Tul (Leguminosae)." reponame:Repositório Institucional da UFBA, 2010. http://www.repositorio.ufba.br/ri/handle/ri/10699.
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CAPES
O presente trabalho relata o estudo químico das cascas das raízes de Caesalpinia pyramidalis Tul., uma árvore pertencente a família Leguminosae endêmica da região da caatinga baiana cujas folhas são utilizadas na medicina popular como diurético e digestivo. O extrato metanólico bruto foi submetido à extração liquido-liquido e sua atividade citotóxica e antioxidante foram avaliadas. O extrato hexânico apresentou-se atóxico para o teste de letalidade de Artemia salina e os demais extratos foram moderadamente tóxicos. Em relação à atividade antioxidante, o extrato AcOEt foi o mais ativo, seguido do BuOH e MeOH. O fracionamento cromatográfico da fase hexânica permitiu o isolamento do lupeol, acacetina, um novo fenilpropanóide, ácido (E)-8-hidroxi-3,5-dimetoxicumarico, além de uma mistura de sitosterol e estigmasterol. Da fase metanólica foram isolados o mesmo fenilpropanóide encontrado na hexânica além de um novo biflavonóide, 7-hidroxi-4’-metoxiflavona-5α-2,4- dihidroxi-4’-metoxidihidrochalcona. A estrutura deste novo biflavonóide bem como das outras substâncias isoladas foram elucidadas a partir da análise dos dados de RMN 1H e 13C (BB e APT) juntamente com a RMN de correlação (HMQC e HMBC).
Salvador
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Fidelis, Queli Cristina. "Metab?litos especiais isolados de folhas e galhos de Ouratea ferruginea Engl. (Ochnaceae)." Universidade Federal Rural do Rio de Janeiro, 2011. https://tede.ufrrj.br/jspui/handle/jspui/1138.
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Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico, CNPq, Brasil.
This work describes the phytochemical study of the stems and leaves of Ouratea ferruginea Engl., Ochnaceae. The material for study was collected in the campus of Embrapa in Bel?m, Par? state, and identified by Dra Silvane Taveres Rodrigues. The compounds described in this phytochemistry investigation were isolated by the solvents partition and chromatographyc techniques of the extracts obtained by maceration at room temperature with hexane, ethyl acetate and methanol. The structures were determined through analysis of data provided by IR, 1H and 13C NMR (1D an 2D techniques), mass spectrometry including GCMS and HPLC-MS of natural compounds and some derivatives. From the stem dichloromethane extract friedelin, friedelinol, sitosterol, stigmasterol, campesterol, 3-b-O-Dglucopyranosyl- stigmasterol, 2,6-dimethoxy?benzoquinine, 2,6-dimethoxy-hydroquinone, the isoflavones: 5,4'-dihydroxy-7,3',5'-trimethoxy-isoflavone, 5-hydroxy-7,3',4',5'-tetramethoxyisoflavone, 5,4'-dihydroxy-7,3'-dimethoxy-isoflavone, 7,5-dihydroxy-3',4',5'-trimethoxyisoflavone, 7,5,4?-trihydroxy-3?,5?-dimethoxy-isoflavone, and ferulic and syringic aldehyde were isolated. From the dichloromethane partition of the methanol extract of the stem vanillic acid, 4 ((1E)-3-hydroxy-1-propenyl)-2-methoxyphenol and 3,5-dimethoxy-4-hydroxydihydrocinamaldehyde were isolated. From hexane fraction of methanol extract from the leaves lupeone was isolated, and from the dichloromethane methanol partition were identified the biflavonoids amentoflavone and 7-methyl-amentoflavone, known as sequoiaflavone, along with syringic acid. From the ethyl acetate of the methanol extracts partition the epicatechin which absolute configuration was defined by circular dichroism spectral analysis was isolated. The sequioflanove is been identified in Ochnaceae for the first time. From the polar fraction the total phenol were determined by adapted Folin-Denis and precipitation with casein methods and by NMR spectral analysis.
Este trabalho descreve o estudo fitoqu?mico de caule e folhas da esp?cie vegetal Ouratea ferruginea Engl, Ochnaceae. O material para estudo foi coletado no campus da Embrapa em Bel?m do Par? e identificado pela Dra Silvane Tavares Rodrigues. As subst?ncias descritas nesta investiga??o fitoqu?mica foram isoladas atrav?s de parti??o com solventes e t?cnicas cromatogr?ficos de extratos obtidos atrav?s de macera??o a frio com hexano, acetato de etila e metanol. As estruturas foram determinadas atrav?s da an?lise de dados fornecidos por espectrometria na regi?o do infravermelho, RMN 1H e 13C (t?cnicas 1D e 2D), de massas incluindo CG-EM e CLAE-EM das subst?ncias naturais e de alguns derivados. Do extrato em diclorometano do caule foram isolados friedelina, friedelinol, sitosterol, estigmasterol, campesterol, 3-b-O-D-glicopiranosil-estigmasterol, 2,6-dimetoxi benzoquinona, 2,6-dimetoxi hidroquinona, as isoflavonas 5,4?-diidroxi-7,3?,5?-trimetoxiisoflavona, 5,4?-diidroxi-7,3?-dimetoxi-isoflavona, 5-hidroxi-7,3?,4?,5?-tetrametoxiisoflavona, 7,5-diidroxi-3?,4?,5?-trimetoxi-isoflavona, 7,5,4?-triidroxi-3?,5?-dimetoxiisoflavona, al?m dos alde?dos sir?ngico e fer?lico. Da parti??o em diclorometano do extrato metan?lico do caule foram isolados ?cido van?lico, 4((1E)-3-hidroxi-1-propenil)-2- metoxifenol e 3,5-dimetoxi-4-hidroxi-diidrocinamalde?do. Das folhas foi isolada a lupeona na parti??o em hexano do extrato metan?lico; e na parti??o em diclorometano foram identificados os biflavon?ides amentoflavona e 7-metil-amentoflavona, conhecida como sequoiaflavona, e o ?cido sir?ngico. Na parti??o em acetato de etila foi isolado a epicatequina cuja configura??o absoluta foi definida com an?lise do espectro de dicro?smo circular. A sequioflavona est? sendo registrada pela primeira vez em Ochnaceae. Das fra??es polares foram determinados o teor de fen?is totais e taninos por m?todos de Folin-Denis e precipita??o com case?na, adaptados, al?m de an?lise com espectros de RMN.
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Fernandes, Daniara Cristina. "Estudo químico e atividade biológica de Garcinia xanthochymus (Clusiaceae) /." Araraquara : [s.n.], 2010. http://hdl.handle.net/11449/97889.
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Orientador: Dulce Helena Siqueira SilvaBanca: Ian Castro Gamboa
Banca: Fernanda Rodrigues Garcez
Resumo: A espécie Garcinia xanthochymus, comumente conhecida como Gamboja, é uma árvore nativa da Índia com aproximadamente 8-10 metros, utilizada extensamente na medicina popular como antidiarréica. Este trabalho descreve o estudo químico e biológico das folhas e frutos de G. xanthochymus. Dentre as substâncias isoladas, podemos destacar 3 triterpenos obtidos do extrato hexânico das folhas: friedelina (1), lanosta-8,24-dien-3-ol (2) e lanosta-7,24-dien-3-ol (3), sendo as substâncias 2 e 3 relatadas pela primeira vez na literatura da espécie. A prospecção química da fase acetato de etila das folhas revelou uma abundante presença de biflavonóides, sendo as substâncias saharanflavona (4), I3, II8-biapigenina (5), GB1a (6), (+)-morelloflavona (8), GB2a (11), volkensiflavona (12), GB2 (14), xantochimusídeo (15) e fukugisídeo (16), caracterizadas pela ligação interflavonoídica do tipo 38'', e as substâncias podocarpusflavona (7) e amentoflavona (9), pela ligação do tipo 3'8''. Merece destaque a substância 4, isolada pela primeira vez de fontes naturais. A composição química de G. xanthochymus constituiu-se ainda das substâncias diidrokaempferol (10), ácido vanílico (13), cinco derivados de ácidos fenilpropanoídicos: ácido 3-O-cafeoilquínico (17), ácido 5-O-cafeoilquínico (18), ácido 3-p-coumaroilquínico (21), ácido 4-O-cafeoilquínico (22) e ácido 4-p-coumaroilquínico (23); e ainda a mistura binária das benzofenonas xantochimol (19) e cicloxantochimol (20). Todas as substâncias foram relatadas pela primeira vez nas folhas de G. xanthochymus, com exceção da substância 7 e as substâncias 10, 13, 17, 18, 21, 22 e 23 ainda não haviam sido identificadas na espécie. Através da técnica CG-DIC foram identificados 15 triterpenos e/ou esteróides nos extratos de baixa polaridade e através das técnicas hifenadas, como CLAE-UV e CLAE-EM, foi ... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The species Garcinia xanthochymus, known as Gamboja is a native Indian tree ca. 8-10 m high, which is extensively used as folk medicine for treating diarrhea and dysentery. This work describes the study of the chemical profile of G. xanthochymus leaves and fruits. Among the isolated substances three triterpenes were obtained from the hexane extract of the leaves: friedelin (1), lanosta-8,24-dien-3-ol (2) e lanosta-7,24-dien-3-ol (3), with compounds (2) and (3) described for the first time in the literature of this species. The chemical prospection of the ethyl acetate extract of the leaves revealed an abundant amount of biflavonoids, with compounds saharanflavone (4), I3,II8-biapigenin (5), GB1a (6), (+)-morelloflavone (8), GB2a (11), volkensiflavone (12), GB2 (14), xanthochymuside (15) and fukugiside (16) characterized by the interflavonoid 38" bond, and compounds podocarpusflavone (7) and amentoflavone (9), by the interflavonoid 3'8'' bond. Substance 4 was isolated for the first time from a natural source. The chemical composition of G. xanthochymus included additionally dihydrokaempferol (10), vanillic acid (13), and five phenylpropanoid acid derivatives: 3-O-caffeoylquinic acid (17), 5-O-caffeoylquinic acid (18), 3-p-coumaroylquinic acid (21), 4-O-caffeoylquinic acid (22) and 4-p-coumaroylquinic acid (23); as well a binary mixture of xanthochymol (19) and cycle-xanthochymol (20) benzophenones. All the compounds were reported for the first time in the leaves of G. xanthochymus, with the exception of compound 7, and compounds 10, 13, 17, 18, 21, 22 e 23, which had not yet been identified in this species. By CG-FID technique fifteen triterpenes and/or steroids were identified in the low polarity extracts. Hyphenated techniques such as HPLC-UV and HPLC-MS were used to locate previously isolated compounds from different fractions and/or isolated substances from other works... (Complete abstract click electronic access below)
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Books on the topic "Biflavonoids"
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Mercader, Andrew G., and Alicia B. Pomilio. Biflavonoids: Occurence, structural features, and bioactivity. Hauppauge, N.Y: Nova Science Publishers, 2011.
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Lone, Shabir Hussain, and Mohd Akbar Khuroo. Biflavonoids: Chemical and Pharmacological Aspects. Elsevier Science & Technology Books, 2016.
Find full textBook chapters on the topic "Biflavonoids"
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Geiger, Hans, and Christopher Quinn. "Biflavonoids." In The Flavonoids, 99–124. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4899-2913-6_4.
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Geiger, Hans. "Biflavonoids and triflavonoids." In The Flavonoids, 95–115. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4899-2911-2_4.
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Hemingway, R. W. "Biflavonoids and Proanthocyanidins." In Natural Products of Woody Plants, 571–651. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-74075-6_17.
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Yue, Tianli, Qinglin Sheng, Yane Luo, Zhengcao Xiao, Yuan Wang, Wei Song, Min Yan, Haili Niu, Ting Zhang, and Na Li. "Biflavonoids and Oligomeric Flavonoids from Food." In Handbook of Dietary Phytochemicals, 1–49. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-13-1745-3_7-1.
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Yue, Tianli, Qinglin Sheng, Yane Luo, Zhengcao Xiao, Yuan Wang, Wei Song, Min Yan, Haili Niu, Ting Zhang, and Na Li. "Biflavonoids and Oligomeric Flavonoids from Food." In Handbook of Dietary Phytochemicals, 155–203. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-4148-3_7.
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Thapa, Arjun, and Eva Y. Chi. "Biflavonoids as Potential Small Molecule Therapeutics for Alzheimer’s Disease." In Advances in Experimental Medicine and Biology, 55–77. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-18365-7_3.
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Brandt, E. Vincent, Riaan Bekker, and Daneel Ferreira. "Structure and Absolute Configuration of Biflavonoids with Benzofuranoid Constituent Units." In Plant Polyphenols 2, 245–58. Boston, MA: Springer US, 1999. http://dx.doi.org/10.1007/978-1-4615-4139-4_13.
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WILLIAMS, CHRISTINE A., and JEFFREY B. HARBORNE. "Biflavonoids." In Methods in Plant Biochemistry, 357–88. Elsevier, 1989. http://dx.doi.org/10.1016/b978-0-12-461011-8.50016-0.
Full textConference papers on the topic "Biflavonoids"
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Jiang, Yong-Mei, Li-Zhi Liang, Tian-Qing Gan, Dan Li, and Gang Wang. "Phytochemistry and Bioactivities of Biflavonoids." In ICBBS '17: 6th International Conference on Bioinformatics and Biomedical Science. New York, NY, USA: ACM, 2017. http://dx.doi.org/10.1145/3121138.3121181.
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Li, Li, Ke-li Chen, Jia-cheng Xu, and Xiang Lei. "Inhibition Action on Xantine Oxidase by Biflavonoids from Selaginella labordei." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2009). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5162968.
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Dziwornu, GA, NR Toorabally, MG Bhowon, S. Jhaumeer-Laulloo, SN Sunassee, A. Moser, T. Salbert, and D. Argyropoulos. "Computer Assisted Structure Elucidation of Two Biflavonoids from the Leaves of Ochna Mauritiana." In GA 2017 – Book of Abstracts. Georg Thieme Verlag KG, 2017. http://dx.doi.org/10.1055/s-0037-1608237.
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Pinanti, Honesty Nurizza, Wirdatun Nafisah, Yuyun Ika Christina, Widodo Widodo, Muhaimin Rifa’i,, and Muhammad Sasmito Djati. "Molecular docking studies of biflavonoids from Selaginella doederleinii hieron as anticancer agents to inhibit mTOR." In INTERNATIONAL CONFERENCE ON LIFE SCIENCES AND TECHNOLOGY (ICoLiST 2020). AIP Publishing, 2021. http://dx.doi.org/10.1063/5.0052704.
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Lou, Jian Shu, Cathy Bi, Gallant Chan, Tina Dong, and Karl Tsim. "Abstract 3325: Ginkgetin, a biflavonoid derived from leaves ofGinkgo biloba, induces autophagic cell death in non-small cell lung cancer via p62." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-3325.
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