Relevant bibliographies by topics / Bile-tolerant

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Academic literature on the topic 'Bile-tolerant'

Author: Grafiati
Published: 4 June 2021
Last updated: 12 February 2022

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Journal articles on the topic "Bile-tolerant"

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Suzuki, Shigenori, Hiromi Kimoto-Nira, Hiroyuki Suganuma, Chise Suzuki, Tadao Saito, and Nobuhiro Yajima. "Cellular fatty acid composition and exopolysaccharide contribute to bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles." Canadian Journal of Microbiology 60, no. 4 (April 2014): 183–91. http://dx.doi.org/10.1139/cjm-2014-0043.

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Bile tolerance is a fundamental ability of probiotic bacteria. We examined this property in 56 Lactobacillus brevis strains isolated from Japanese pickles and also evaluated cellular fatty acid composition and cell-bound exopolysaccharide (EPS-b) production. The bile tolerance of these strains was significantly lower in modified de Man – Rogosa – Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium. Aggregating strains showed significantly higher bile tolerance than nonaggregating strains in MRS medium, but there was no significant difference in the modified MRS media. The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile. In MRS without sodium acetate, only the 3 most tolerant nonaggregating strains increased their relative C18:1 content in the presence of bile. Meanwhile, culture in MRS without sodium acetate reduced EPS-b production in aggregating strains. In conclusion, both EPS-b and cellular fatty acid composition play important roles in bile tolerance of pickle-derived L. brevis.
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Mulaw, Guesh, Tesfaye Sisay Tessema, Diriba Muleta, and Anteneh Tesfaye. "In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some Traditionally Fermented Ethiopian Food Products." International Journal of Microbiology 2019 (August 25, 2019): 1–11. http://dx.doi.org/10.1155/2019/7179514.

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Probiotics are live microorganisms which when consumed in large number together with a food promote the health of the consumer. The aim of this study was to evaluate in vitro probiotic properties of lactic acid bacteria (LAB) isolated from traditional Ethiopian fermented Teff injera dough, Ergo, and Kocho products. A total of 90 LAB were isolated, of which 4 (4.44%) isolates showed 45.35–97.11% and 38.40–90.49% survival rates at pH values (2, 2.5, and 3) for 3 and 6 h, in that order. The four acid-tolerant isolates were found tolerant to 0.3% bile salt for 24 h with 91.37 to 97.22% rate of survival. The acid-and-bile salt-tolerant LAB isolates were found inhibiting some food-borne test pathogenic bacteria to varying degrees. All acid-and-bile-tolerant isolates displayed varying sensitivity to different antibiotics. The in vitro adherence to stainless steel plates of the 4 screened probiotic LAB isolates were ranged from 32.75 to 36.30% adhesion rate. The four efficient probiotic LAB isolates that belonged to Lactobacillus species were identified to the strain level using 16S rDNA gene sequence comparisons and, namely, were Lactobacillus plantarum strain CIP 103151, Lactobacillus paracasei subsp. tolerans strain NBRC 15906, Lactobacillus paracasei strain NBRC 15889, and Lactobacillus plantarum strain JCM 1149. The four Lactobacillus strains were found to be potentially useful to produce probiotic products.
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CHARTERIS, WILLIAM P., PHILLIP M. KELLY, LORENZO MORELLI, and J. KEVIN COLLINS. "Effect of Conjugated Bile Salts on Antibiotic Susceptibility of Bile Salt–Tolerant Lactobacillus and Bifidobacterium Isolates." Journal of Food Protection 63, no. 10 (October 1, 2000): 1369–76. http://dx.doi.org/10.4315/0362-028x-63.10.1369.

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Virtually every antibiotic may cause in vivo alterations in the number, level, and composition of the indigenous microbiotae. The degree to which the microbiotae are disturbed depends on many factors. Although bile may augment antibiotic activity, studies on the effect of bile on the antibiotic susceptibility of indigenous and exogenous probiotic microorganisms are lacking. It was against this background that the antibiotic susceptibility of 37 bile salt–tolerant Lactobacillus and 11 Bifidobacterium isolates from human and other sources was determined in the presence of 0.5% wt/wt oxgall (conjugated bile salts). Oxgall did not affect the intrinsic resistance of lactobacilli to metronidazole (5 μg), vancomycin (30 μg), and cotrimoxazole (25 μg), whereas it resulted in a complete loss of resistance to polymyxin B (300 μg) and the aminoglycosides gentamicin (10 μg), kanamycin (30 μg), and streptomycin (10 μg) for most strains studied (P < 0.001). Oxgall did not affect the intrinsic resistance of bifidobacteria to metronidazole and vancomycin, whereas polymyxin B and co-trimoxazole resistance was diminished (P < 0.05) and aminoglycoside resistance was lost (P < 0.001). Seven lactobacilli, but no bifidobacteria strain, showed unaltered intrinsic antibiotic resistance profiles in the presence of oxgall. Oxgall affected the extrinsic susceptibility of lactobacilli and bifidobacteria to penicillin G (10 μg), ampicillin (10 μg), tetracycline (30 μg), chloramphenicol (30 μg), erythromycin (15 μg), and rifampicin (5 μg) in a source- and strain-dependent manner. Human strain–drug combinations of lactobacilli (P < 0.05) and bifidobacteria (P < 0.01) were more likely to show no change or decreased susceptibility compared with other strain-drug combinations. The antimicrobial activity spectra of polymyxin B and the aminoglycosides should not be considered limited to gram-negative bacteria but extended to include gram-positive genera of the indigenous and transiting microbiotae in the presence of conjugated bile salts. Those lactobacilli (7 of 37) that show unaltered intrinsic and diminished extrinsic antibiotic susceptibility in the presence of oxgall may possess greater upper gastrointestinal tract transit tolerance in the presence of antibiotics.
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Kumar, M., P. K. Sharma, P. K. Sharma, and N. Kumar. "Molecular identification of bile tolerant Lactobacillus fermentum strains isolated from Human Faeces." International Journal of Agricultural Invention 1, no. 02 (December 31, 2016): 182–89. http://dx.doi.org/10.46492/ijai/2016.1.2.11.

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Conventional procedures of identification are laborious and time consuming because they depend on growth of microorganism in that particular environment and hence, sometimes, give misleading results while molecular methods relies upon generating a large number of copies of a specific part of its genome for identification of unknown isolates and enables the problem of poor identification. The identification of a microbial isolate to genus level imparts a partial characterization of the isolate revealing a lot of information about that organism. Lactic acid bacteria are very significant to human health due to their probiotic attributes, production of antimicrobial substances and subsequently to inhibit pathogenic bacteria. Bile tolerance has been one of the major probiotic criterion while selecting the isolate for probiotic properties. This work aims at looking directly at the genome of bile tolerant Lactobacillus isolates using genotypic and phenotypic characteristics. The isolates were identified by sequencing specific region of 16 S r DNA segment, after amplification by PCR, and then comparing the results to sequences from related database. The results from both conventional and molecular methods were then compared. Twenty five isolates (25) of Lactobacillus fermentum were isolated from fecal samples and identified using API- 50 CH. The isolates were further identified using molecular methods using polymerase chain reaction (PCR) for amplification of 16S rRNA gene to confirm their identities. The genotypic characterization however showed that 84% of the organisms identified using conventional method as L. fermentum correlated, while 16% did not correlate; 2 were identified as Lactobacillus planterum, 01 as L. delbrueckiiand 01 as L.paraplanterum.
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Chen, Sheng, Yu Li, Wen Bin Jin, Yan Chen, Xiao Guang Liu, and Fu Ping Lu. "Selective Breeding of Oxygen-Tolerant and Oxalate-Degrading Lactic Acid Bacteria by Protoplast Fusion." Advanced Materials Research 750-752 (August 2013): 1489–94. http://dx.doi.org/10.4028/www.scientific.net/amr.750-752.1489.

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Bifidobacterium lactiswith oxalate-degrading capacity can efficiently reduce the oxalate in vivo, and it can be used to prevent and treat kidney stone diseases. WhileBifidobacterium lactisis poorly oxygen-tolerant, which hinders it from being as microbial ecological agents. To obtain oxygen-tolerant and oxalate-degrading lactic acid bacteria, protoplast fusion technology was used betweenB. lactisandL. acidophilus.Under the optimum conditions of protoplast fusion with PEG 6000 concentration 50%, the fusion time 7 min, the fusion temperature 30°C, the concentration of CaCl20. 02mol/ L and the concentration of MgCl20.5mol/ L, the fusion rate reached 7.6%, and three oxygen-tolerant fusant showing that the level of oxalate degradation were similar withB. lactiswas obtained. The fusants of SZY1-7 and SZY2-1 could tolerance to pH 2.5 and 0.5% (w/v) bile salt.
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Menconi, Anita, Marion J. Morgan, Neil R. Pumford, Billy M. Hargis, and Guillermo Tellez. "Physiological Properties and Salmonella Growth Inhibition of Probiotic Bacillus Strains Isolated from Environmental and Poultry Sources." International Journal of Bacteriology 2013 (May 26, 2013): 1–8. http://dx.doi.org/10.1155/2013/958408.

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The objective of the present study was to describe the physiological properties of seven potential probiotic strains of Bacillus spp. Isolates were characterized morphologically, biochemically, and by 16S rRNA sequence analyses for identification. Tolerance to acidic pH, high osmotic concentrations of NaCl, and bile salts were tested. Isolates were also evaluated for their ability to metabolize different carbohydrates sources. The antimicrobial sensitivity profiles were determined. Inhibition of gastrointestinal Salmonella colonization in an avian model was also evaluated. Five strains of Bacillus were tolerant to acidic conditions (pH 2.0) and all strains were tolerant to a high osmotic pressure (NaCl at 6.5%). Moreover, all strains were able to tolerate concentration of 0.037% bile salts after 24 h of incubation. Three strains were able to significantly reduce Salmonella Typhimurium levels in the crop and in the ceca of broiler-type chickens. Among the 12 antibiotics tested for antibiotic resistance, all strains were resistant to bacitracin and susceptible to gentamycin, neomycin, ormethoprim, triple sulfa, and spectinomycin. Bacterial spore formers have been shown to prevent gastrointestinal diseases in animals and humans. The results obtained in this study show important characteristics to be evaluated when selecting Bacillus spp. candidates to be used as probiotics.
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Chou, Lan-Szu, and Bart Weimer. "Isolation and Characterization of Acid- and Bile-Tolerant Isolates from Strains of Lactobacillus acidophilus." Journal of Dairy Science 82, no. 1 (January 1999): 23–31. http://dx.doi.org/10.3168/jds.s0022-0302(99)75204-5.

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Bae, H. C., M. S. Nam, and J. Y. Lee. "Probiotic Characterization of Acid- and Bile-tolerant Lactobacillus salivarius subsp. salivarius from Korean Faeces." Asian-Australasian Journal of Animal Sciences 15, no. 12 (January 1, 2002): 1798–807. http://dx.doi.org/10.5713/ajas.2002.1798.

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OTSUKA, Makoto, Tomoko MUTO, Yu KATSUNUMA, Yutaka NAKAMURA, Ryozo TAKADA, and Hajime MINATO. "Effect of administration of acid- and bile-tolerant lactobacilli on pigs intestinal microbial population." Animal Science Journal 73, no. 6 (December 2002): 497–503. http://dx.doi.org/10.1046/j.1344-3941.2002.00068.x.

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10

Watson, Debbie, Roy D. Sleator, Pat G. Casey, Colin Hill, and Cormac G. M. Gahan. "Specific Osmolyte Transporters Mediate Bile Tolerance in Listeria monocytogenes." Infection and Immunity 77, no. 11 (September 8, 2009): 4895–904. http://dx.doi.org/10.1128/iai.00153-09.

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ABSTRACT The food-borne pathogenic bacterium Listeria monocytogenes has the potential to adapt to an array of suboptimal growth environments encountered within the host. The pathogen is relatively bile tolerant and has the capacity to survive and grow within both the small intestine and the gallbladder in murine models of oral infection. We have previously demonstrated a role for the principal carnitine transport system of L. monocytogenes (OpuC) in gastrointestinal survival of the pathogen (R. Sleator, J. Wouters, C. G. M. Gahan, T. Abee, and C. Hill, Appl. Environ. Microbiol. 67:2692-2698, 2001). However, the mechanisms by which OpuC, or indeed carnitine, protects the pathogen in this environment are unclear. In the current study, systematic analysis of strains with mutations in osmolyte transporters revealed a role for OpuC in resisting the acute toxicity of bile, with a minor role also played by BetL, a secondary betaine uptake system which also exhibits a low affinity for carnitine. In addition, the toxic effects of bile on wild-type L. monocytogenes cells were ameliorated when carnitine (but not betaine) was added to the medium. lux-promoter fusions to the promoters of the genes encoding the principal osmolyte uptake systems Gbu, BetL, and OpuC and the known bile tolerance system BilE were constructed. Promoter activity for all systems was significantly induced in the presence of bile, with the opuC and bilE promoters exhibiting the highest levels of bile-dependent expression in vitro and the betL and bilE promoters showing the highest expression levels in the intestines of orally inoculated mice. A direct comparison of all osmolyte transporter mutants in a murine oral infection model confirmed a major role for OpuC in intestinal persistence and systemic invasion and a minor role for the BetL transporter in fecal carriage. This study therefore demonstrates a previously unrecognized function for osmolyte uptake systems in bile tolerance in L. monocytogenes.
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Dissertations / Theses on the topic "Bile-tolerant"

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Chou, Lan-Szu. "Isolation and Biochemical Characterization of Acid- and Bile- Tolerant Strains of Lactobacillus Acidophilus and Bifidobacterium Bifidum." DigitalCommons@USU, 1997. https://digitalcommons.usu.edu/etd/5427.

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Lactic acid bacteria have been reported to be used as a health adjunct in food for u many years. However, these health benefits have not been proven. and how these bacteria pass through the digestion process and remain viable in the human intestinal tract is still not clear. The aim of this work was to isolate mutants from Lactobacillus acidophilus or Bifidobacterium bifidum that could tolerate the conditions of the digestion process (low pH and bile conduction) and to characterize these isolated mutants. Acid- and bile-tolerant mutants of L. acidophilus were isolated from parental strains successfully using natural selection techniques. These mutants survived and grew at conditions of pH 3.5 with 0.2% mixed bile salts added. After the selection, phenotypic characterization was identified to further clarify desirable traits for use as probiotic adjuncts in foods. These phenotypic characteristics included protease, aminopeptidase, ß-galactosidase, and bile salt hydrolase activity. Based on different protease, aminopeptidase, and ß-galactosidase activity, selected acid- and bile-tolerant mutants contained different growth characteristics compared with their parents. All the isolates tested showed different bile salt hydrolase activity, and this activity was not strain and medium dependent. Plasmid profiles and fatty acid analysis were conducted to provide more information of these acid/bile tolerant isolates and whether or not they were mutants from their parent strains rather than only adapted variants. Results showed the acid-/bile-tolerant isolates contained different plasmid profiles and cell wall fatty acids compared with their parents, which indicated these isolates were mutants. Protein expression by two-dimensional gel electrophoresis showed different protein expression patterns between acid- and bile-tolerant mutants and their parents. fm1her suggesting these isolates were mutants. We observed the protein production in parent strains decreased as the pH decreased. and protein expression in mutants remained the same as pH decreased. Two of the proposed health benefits of probiotic bacteria are anticholesterol activity and antimicrobial activity. These were evaluated using selected acid- and bile-tolerant mutants. Results showed no decrease of cholesterol in the test medium during bacterial growth. The observed antimicrobial activity was due to the presence of active cells. and this may relate to the acid production during cell growth and not to the production of antimicrobial substances. We concluded that the acid-/bile-tolerant isolates were mutants, and they survived and grew better in harsh environments compared with their parent strains. These mutants may be useful as a food adjunct in the future, but further study is needed to establish their use and possible probiotic benefits.
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XU, KUN-DI, and 許坤地. "Isolation and identification of acid and bile salt tolerant high adherence lactic acid bacteria." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/n2a3f6.

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碩士
國立屏東科技大學
食品生技碩士學位學程在職專班
105
Lactic acid bacteria and human diet are closely related, since ancient times that is used in the preservation and manufacture of food, including meat processing, dairy fermentation and a variety of pickled products. And many intestinal bacteria, lactic acid bacteria for probiotics on behalf of, with improved intestinal bacteria phase, lower body cholesterol, reduce fatty liver, improve lactose intolerance, strengthen the immune system and anti-tumor effect, but probiotics After eating into the body, then facing the challenges of digestive system, such as: intestinal peristalsis, digestive fluid flow, bile salt, stomach acid and lysozyme etc., can overcome and colonize the survival of the intestine, and become dominant bacteria In order to achieve the role of improving the health of the human body, but few of the current capacity of the lactic acid bacteria were found, so the experiment from different types of fruits and vegetables, fermented food desktop kimchi, Korean kimchi, stinky tofu, salted gourd, broken cloth, banana stems, 0.5 % (w / v) CaCO3 MRS medium, and the purified strains were isolated and subjected to microscopic examination, catalase test, potassium hydroxide reaction, curd test and Gram Dyeing, the initial determination of lactic acid bacteria, and then with Lactobacillus acidophilus BCRC10695 and Lactobacillus paracasei subsp BCRC14023 acid, bile Continuous adsorption capacity and tolerance test comparing, then to API50 strain identification and 16s RNA sequencing. The results showed that Lactobacillus fermentum, which was able to screen out the lactic acid bacteria ( NO.4 ), was resistant to bile salt ( NO.39 ) and continuous tolerance ( NO.38 ) Lactobacillus reuteri.According to the above research results, due to Lactobacillus reuteri resistance to harsh digestive environment, so the follow-up will be lactic acid bacteria for product development, look forward to the future to provide people with effective improvement of intestinal bacteria phase lactic acid bacteria products.
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Book chapters on the topic "Bile-tolerant"

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Ahmed, Siham A., Salam A. Ibrahim, Chyer Kim, and Abolghasem Shahbazi. "Significance of Bile Salt Tolerant Lactobacillus reuteri." In Proceedings of the 2007 National Conference on Environmental Science and Technology, 17–23. New York, NY: Springer New York, 2009. http://dx.doi.org/10.1007/978-0-387-88483-7_3.

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