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Relevant bibliographies by topics / Bio-assay guided fractionation

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Journal articles

Academic literature on the topic 'Bio-assay guided fractionation'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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Journal articles on the topic "Bio-assay guided fractionation"

1

Sánchez, Luis A., Zeuz Capitan, Luz I. Romero, Eduardo Ortega-Barría, William H. Gerwick, and Luis Cubilla-Rios. "Bio-Assay Guided Isolation of Germacranes with Anti-Protozoan Activity from Magnolia sororum." Natural Product Communications 2, no. 11 (2007): 1934578X0700201. http://dx.doi.org/10.1177/1934578x0700201101.

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In the course of our search for antiprotozoal agents from terrestrial plants, three new germacranes 1, 2 and 3, together with the well known sesquiterpene lactones parthenolide (4) and costunolide (5), were isolated from Magnolia sororum using bioassay-guided fractionation methods. The structures of these new compounds were elucidated by 1D and 2D NMR spectroscopic analysis. Compound 5 exhibited activity (IC50 = 9.4 μM) in vitro against the Leishmania mexicana parasite. Additionally, all compounds were also evaluated against Trypanosoma cruzi and Monkey Vero cells without showing much activity

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Dirsch, Verena, Hermann Stuppner, and Angelika Vollmar. "The Griess Assay: Suitable for a Bio-Guided Fractionation of Anti-Inflammatory Plant Extracts?" Planta Medica 64, no. 05 (1998): 423–26. http://dx.doi.org/10.1055/s-2006-957473.

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Chong, Zhen Yee, Sylvia Sandanamsamy, Nur Najihah Ismail, Suriyati Mohamad, and Khayriyyah Mohd Hanafiah. "Bio-Guided Fractionation of Oil Palm (Elaeis guineensis) Fruit and Interactions of Compounds with First-Line Antituberculosis Drugs against Mycobacterium tuberculosis H37Ra." Separations 8, no. 2 (2021): 19. http://dx.doi.org/10.3390/separations8020019.

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Natural products with antimycobacterial adjuvant potential may be utilized to address the rise of multidrug-resistant tuberculosis (TB). The antioxidant-rich oil palm (Elaeis guineensis) fruit (OPF) was investigated for antimycobacterial activity against Mycobacterium tuberculosis (MTB) H37Ra using bio-guided fractionation techniques, followed by determination of fractional inhibition index (FIC) with first-line anti-TB drugs. In vitro screening using microplate Alamar blue assay showed n-hexane and chloroform partitions of OPF mesocarp had a minimum inhibitory concentration (MIC) of 400–800 µ

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Rajbhandari, M., and Th Schoepke. "Characterization of the Influenza Virus Inhibiting Fractions of Bergenia ligulata." Nepal Journal of Science and Technology 12 (July 23, 2012): 290–95. http://dx.doi.org/10.3126/njst.v12i0.6515.

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In the course of screening of plant extracts for anti-influenza viral activity, 50% aqueous methanolic extract exhibited antiviral activity against influenza virus A. Bio-assay guided fractionation of the extract led to the indication of the presence of condensed tannins as the active constituents. This was confirmed by chemical tests as well as by thin layer chromatographic comparison with known tannin containing plant extracts. The active fractions were also analysed for the presence of carbohydrates by gas chromatography.DOI: http://dx.doi.org/10.3126/njst.v12i0.6515 Nepal Journal of Scienc

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Capetti, Francesca, Cecilia Cagliero, Arianna Marengo, Carlo Bicchi, Patrizia Rubiolo та Barbara Sgorbini. "Bio-Guided Fractionation Driven by In Vitro α-Amylase Inhibition Assays of Essential Oils Bearing Specialized Metabolites with Potential Hypoglycemic Activity". Plants 9, № 9 (2020): 1242. http://dx.doi.org/10.3390/plants9091242.

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Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by unpaired blood glycaemia maintenance. T2DM can be treated by inhibiting carbohydrate hydrolyzing enzymes (α-amylases and α-glucosidases) to decrease postprandial hyperglycemia. Acarbose and voglibose are inhibitors used in clinical practice. However, these drugs are associated with unpleasant gastrointestinal side effects. This study explores new α-amylase inhibitors deriving from plant volatile specialized metabolites. Sixty-two essential oils (EOs) from different plant species and botanical families were subjected to α-

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Hussain, Fatma, and Fatima Ikram. "Antioxidant and Antidiabetic Potential of Saponin Fraction Isolated from Moringa oleifera Leaves." Biological Sciences - PJSIR 63, no. 2 (2020): 86–92. http://dx.doi.org/10.52763/pjsir.biol.sci.63.2.2020.86.92.

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To assess total phenolic contents, total flavonoid contents (TPC, TFC), DPPH scavenging and antidiabetic (antiglycation and alpha amylase hindrance) activities of Moringa oleifera (MO) leaves, bio- assay guided fractionation was used with thin layer chromatography profile to isolate saponin fraction (SF). The TPC and TFC were 9.7-12.8 GAE and 5.3-9.3 CE (g/100 g dry weight), respectively and aqueous and methanol fractions were more effective. DPPH scavenging activity ranged 69.5-83.1%. SF displayed noteworthy antioxidant ability (p<0.046) and it was leading inhibitor of glycation (p<0.04

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7

Shaheen, Nargis, Naveeda Akhter Qureshi, Attiya Iqbal, Asma Ashraf, and Huma Fatima. "Evaluation of Safety, Antileishmanial, and Chemistry of Ethanolic Leaves Extracts of Seven Medicinal Plants: An In-vitro Study." Open Chemistry Journal 7, no. 1 (2020): 26–36. http://dx.doi.org/10.2174/1874842202007010026.

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Background: Cutaneous leishmaniasis is a neglected tropical disease that currently affects people among 98 countries and causes significant morbidity and mortality. Current chemotherapeutic intervention is unsatisfactory and has various limitations that highlight the necessity to develop safe and effective therapeutic approaches from natural products. Objective: The main objective of current study was the evaluation of the antileishmanial activity along with toxicity assessment of selected plant extracts. Methods: The ethanolic leaves extracts of selected plants were evaluated for their qualit

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8

Hoang Anh, La, Tran Dang Xuan, Nguyen Thi Dieu Thuy, Nguyen Van Quan та Le Thu Trang. "Antioxidant and α-amylase Inhibitory Activities and Phytocompounds of Clausena indica Fruits". Medicines 7, № 3 (2020): 10. http://dx.doi.org/10.3390/medicines7030010.

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Background: Clausena indica fruit is commonly used for food ingredients and traditional medicines in tropical countries, however, information about its biological activities and chemical profiles has been inadequately reported. Methods: In this study, a bio-guided fractionation of antioxidants and α-amylase inhibitors from hexane (MH) and ethyl acetate (ME) extracts of C. indica fruit (pericarp and seed) was carried out. Eleven fractions from MH (D1–D11) and 17 fractions from ME (T1–T17) were obtained from column chromatography over silica gel, which were then examined for anti-radical capacit

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9

Quan, Nguyen Van, Tran Dang Xuan, La Hoang Anh, and Hoang-Dung Tran. "Bio-Guided Isolation of Prospective Bioactive Constituents from Roots of Clausena indica (Dalzell) Oliv." Molecules 24, no. 24 (2019): 4442. http://dx.doi.org/10.3390/molecules24244442.

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Clausena indica fruits are routinely used for the culinary purpose as natural spices, whereas leaves and roots are folk medicine with various health benefits in southern China, South and Southeast Asia. In this study, the bioassay-guided fractionation by column chromatography yielded three pure compounds including dentatin, nordentatin, and clausine K and five active fractions (Re1-5) from C. indica roots. These known anticancer compounds were confirmed by X-ray diffraction, 1H-, 13C-nuclear magnetic resonance (NMR), and electrospray ionization tandem mass spectrometric (ESI-MS-MS) analyses. M

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10

Ahmed Hassanali, Joseph Owino. "Bio-Assay Guided Fractionation of Anti-Mosquito Limonoids from Turraea abyssinica and Turraea cornucopia." Journal of Biofertilizers & Biopesticides 05, no. 01 (2014). http://dx.doi.org/10.4172/2155-6202.1000142.

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