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Journal articles on the topic 'Bio-orthogonal labeling'

Author: Grafiati

Published: 10 September 2021

Last updated: 30 July 2025

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1

Németh, Krisztina, Zsófia László, Adrienn Biró, et al. "Organic Anion Transporting Polypeptide 3A1 (OATP3A1)-Gated Bio-Orthogonal Labeling of Intracellular Proteins." Molecules 28, no. 6 (2023): 2521. http://dx.doi.org/10.3390/molecules28062521.

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Organic anion transporting polypeptides (OATPs) were found to readily deliver membrane impermeable, tetrazine bearing fluorescent probes into cells. This feature was explored in OATP3A1 conditioned bio-orthogonal labeling schemes of various intracellular proteins in live cells. Confocal microscopy and super-resolution microscopy (STED) studies have shown that highly specific and efficient staining of the selected intracellular proteins can be achieved with the otherwise non-permeable probes when OATP3A1 is present in the cell membrane of cells. Such a transport protein linked bio-orthogonal la

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2

Chang, Wei-Hau, and Yuhua Liu. "Bio-Orthogonal Protein Labeling Methods for Single Molecule FRET." Journal of the Chinese Chemical Society 57, no. 3B (2010): 505–13. http://dx.doi.org/10.1002/jccs.201000073.

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3

Best, Marcel, Isabel Porth, Sebastian Hauke, Felix Braun, Dirk-Peter Herten, and Richard Wombacher. "Protein-specific localization of a rhodamine-based calcium-sensor in living cells." Organic & Biomolecular Chemistry 14, no. 24 (2016): 5606–11. http://dx.doi.org/10.1039/c6ob00365f.

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4

Ahrens, Nicole, Enno Aeissen, Anka Lippe, Ulrike Janssen-Bienhold, Jens Christoffers, and Karl-Wilhelm Koch. "Farnesylation of Zebrafish G-Protein-Coupled Receptor Kinase Using Bio-orthogonal Labeling." ACS Chemical Neuroscience 12, no. 10 (2021): 1824–32. http://dx.doi.org/10.1021/acschemneuro.1c00155.

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5

Wolfe, Michael B., Aaron C. Goldstrohm, and Peter L. Freddolino. "Global analysis of RNA metabolism using bio-orthogonal labeling coupled with next-generation RNA sequencing." Methods 155 (February 2019): 88–103. http://dx.doi.org/10.1016/j.ymeth.2018.12.001.

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6

Evans, Eric G. B., Subhashis Jana, Hyo Sang Jang, et al. "Accessing protein conformational distributions in mammalian cells with fast, bio-orthogonal spin-labeling and DEER spectroscopy." Biophysical Journal 121, no. 3 (2022): 406a. http://dx.doi.org/10.1016/j.bpj.2021.11.728.

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7

Ullrich, Milena, Vanessa Liang, Yee Lian Chew, et al. "Bio-orthogonal labeling as a tool to visualize and identify newly synthesized proteins in Caenorhabditis elegans." Nature Protocols 9, no. 9 (2014): 2237–55. http://dx.doi.org/10.1038/nprot.2014.150.

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8

Shalizi, Aryaman, Toni N. Wiegers, and Hédia Maamar. "Click-to-Capture: A method for enriching viable Staphylococcus aureus using bio-orthogonal labeling of surface proteins." PLOS ONE 15, no. 6 (2020): e0234542. http://dx.doi.org/10.1371/journal.pone.0234542.

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9

Hu, Fang, Youyong Yuan, Wenbo Wu, Duo Mao, and Bin Liu. "Dual-Responsive Metabolic Precursor and Light-Up AIEgen for Cancer Cell Bio-orthogonal Labeling and Precise Ablation." Analytical Chemistry 90, no. 11 (2018): 6718–24. http://dx.doi.org/10.1021/acs.analchem.8b00547.

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10

Nguyen, Duy P., Thomas Elliott, Matthew Holt, Tom W. Muir, and Jason W. Chin. "Genetically Encoded 1,2-Aminothiols Facilitate Rapid and Site-Specific Protein Labeling via a Bio-orthogonal Cyanobenzothiazole Condensation." Journal of the American Chemical Society 133, no. 30 (2011): 11418–21. http://dx.doi.org/10.1021/ja203111c.

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11

Ullrich, Milena, Vanessa Liang, Yee Lian Chew, et al. "Erratum: Corrigendum: Bio-orthogonal labeling as a tool to visualize and identify newly synthesized proteins in Caenorhabditis elegans." Nature Protocols 9, no. 12 (2014): 2903. http://dx.doi.org/10.1038/nprot1214-2903c.

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12

Huber, Thomas, and Thomas P. Sakmar. "Novel Trifunctional Bio-Orthogonal Reagents for Microscale Stoichiometric Labeling of Proteins for Single-Molecule Fluorescence Studies of Signalosomes." Biophysical Journal 100, no. 3 (2011): 256a—257a. http://dx.doi.org/10.1016/j.bpj.2010.12.1618.

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13

Zhang, Pengfei, Tao Jiang, Yuanyuan Li, et al. "Bio‐orthogonal AIE Dots Based on Polyyne‐Bridged Red‐emissive AIEgen for Tumor Metabolic Labeling and Targeted Imaging." Chemistry – An Asian Journal 14, no. 6 (2018): 770–74. http://dx.doi.org/10.1002/asia.201801609.

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14

Hu, Fang, Duo Mao, Kenry, et al. "A Light-Up Probe with Aggregation-Induced Emission for Real-Time Bio-orthogonal Tumor Labeling and Image-Guided Photodynamic Therapy." Angewandte Chemie 130, no. 32 (2018): 10339–43. http://dx.doi.org/10.1002/ange.201805446.

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15

Hu, Fang, Duo Mao, Kenry, et al. "A Light-Up Probe with Aggregation-Induced Emission for Real-Time Bio-orthogonal Tumor Labeling and Image-Guided Photodynamic Therapy." Angewandte Chemie International Edition 57, no. 32 (2018): 10182–86. http://dx.doi.org/10.1002/anie.201805446.

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16

van Gelder, Charlotte A. G. H., Renske Penning, Tim S. Veth, et al. "Temporal Quantitative Proteomics of mGluR-induced Protein Translation and Phosphorylation in Neurons." Molecular & Cellular Proteomics 19, no. 12 (2020): 1952–67. http://dx.doi.org/10.1074/mcp.ra120.002199.

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At neuronal synapses, activation of group I metabotropic glutamate receptors (mGluR1/5) triggers a form of long-term depression (mGluR-LTD) that relies on new protein synthesis and the internalization of AMPA-type glutamate receptors. Dysregulation of these processes has been implicated in the development of mental disorders such as autism spectrum disorders and therefore merit a better understanding on a molecular level. Here, to study mGluR-induced signaling pathways, we integrated quantitative phosphoproteomics with the analyses of newly synthesized proteins via bio-orthogonal amino acids (

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17

Jecmen, Tomas, Roman Tuzhilkin, and Miroslav Sulc. "Photo-Methionine, Azidohomoalanine and Homopropargylglycine Are Incorporated into Newly Synthesized Proteins at Different Rates and Differentially Affect the Growth and Protein Expression Levels of Auxotrophic and Prototrophic E. coli in Minimal Medium." International Journal of Molecular Sciences 24, no. 14 (2023): 11779. http://dx.doi.org/10.3390/ijms241411779.

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Residue-specific incorporation of non-canonical amino acids (ncAAs) introduces bio-orthogonal functionalities into proteins. As such, this technique is applied in protein characterization and quantification. Here, we studied protein expression with three methionine analogs, namely photo-methionine (pMet), azidohomoalanine (Aha) and homopropargylglycine (Hpg), in prototrophic E. coli BL-21 and auxotrophic E. coli B834 to maximize ncAA content, thereby assessing the effect of ncAAs on bacterial growth and the expression of cytochrome b5 (b5M46), green fluorescence protein (MBP-GFP) and phage sho

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18

Herrmann, Alexandra, Doris Jungnickl, Arne Cordsmeier, Antonia Sophia Peter, Klaus Überla, and Armin Ensser. "Cloning of a Passage-Free SARS-CoV-2 Genome and Mutagenesis Using Red Recombination." International Journal of Molecular Sciences 22, no. 19 (2021): 10188. http://dx.doi.org/10.3390/ijms221910188.

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The ongoing pandemic coronavirus (CoV) disease 2019 (COVID-19) by severe acute respiratory syndrome CoV-2 (SARS-CoV-2) has already caused substantial morbidity, mortality, and economic devastation. Reverse genetic approaches to generate recombinant viruses are a powerful tool to characterize and understand newly emerging viruses. To contribute to the global efforts for countermeasures to control the spread of SARS-CoV-2, we developed a passage-free SARS-CoV-2 clone based on a bacterial artificial chromosome (BAC). Moreover, using a Lambda-based Red recombination, we successfully generated diff

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19

Kranaster, Petra, Christiaan Karreman, Jeremias E. G. A. Dold, et al. "Time and space-resolved quantification of plasma membrane sialylation for measurements of cell function and neurotoxicity." Archives of Toxicology 94, no. 2 (2019): 449–67. http://dx.doi.org/10.1007/s00204-019-02642-z.

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AbstractWhile there are many methods to quantify the synthesis, localization, and pool sizes of proteins and DNA during physiological responses and toxicological stress, only few approaches allow following the fate of carbohydrates. One of them is metabolic glycoengineering (MGE), which makes use of chemically modified sugars (CMS) that enter the cellular biosynthesis pathways leading to glycoproteins and glycolipids. The CMS can subsequently be coupled (via bio-orthogonal chemical reactions) to tags that are quantifiable by microscopic imaging. We asked here, whether MGE can be used in a quan

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20

Wu, K., M. He, I. Khan, et al. "Bio-orthogonal chemistry-based method for fluorescent labelling of ribosomal RNA in live mammalian cells." Chemical Communications 55, no. 70 (2019): 10456–59. http://dx.doi.org/10.1039/c9cc05346h.

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21

Brieke, Clara, Grace Yim, Madeleine Peschke, Gerard D. Wright, and Max J. Cryle. "Catalytic promiscuity of glycopeptide N-methyltransferases enables bio-orthogonal labelling of biosynthetic intermediates." Chemical Communications 52, no. 94 (2016): 13679–82. http://dx.doi.org/10.1039/c6cc06975d.

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22

Goyard, David, Peremobowei Iyanu Diriwari, and Nathalie Berthet. "Metabolic labelling of cancer cells with glycodendrimers stimulate immune-mediated cytotoxicity." RSC Medicinal Chemistry 13, no. 1 (2022): 72–78. http://dx.doi.org/10.1039/d1md00262g.

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23

Schnell, Simon D., Lukas V. Hoff, Advaita Panchagnula, et al. "3-Bromotetrazine: labelling of macromolecules via monosubstituted bifunctional s-tetrazines." Chemical Science 11, no. 11 (2020): 3042–47. http://dx.doi.org/10.1039/c9sc06169j.

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24

Lam, Yuen Yi, Angel Tan, Kristian Kempe, and Ben J. Boyd. "Metabolic glycan labelling with bio-orthogonal targeting and its potential in drug delivery." Journal of Controlled Release 378 (February 2025): 880–98. https://doi.org/10.1016/j.jconrel.2024.12.018.

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25

Kozma, Eszter, Orsolya Demeter, and Péter Kele. "Bio-orthogonal Fluorescent Labelling of Biopolymers through Inverse-Electron-Demand Diels-Alder Reactions." ChemBioChem 18, no. 6 (2017): 486–501. http://dx.doi.org/10.1002/cbic.201600607.

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26

Kozma, Eszter, Orsolya Demeter, and Péter Kele. "Corrigendum: Bio-orthogonal Fluorescent Labelling of Biopolymers through Inverse-Electron-Demand Diels-Alder Reactions." ChemBioChem 18, no. 6 (2017): 570. http://dx.doi.org/10.1002/cbic.201700096.

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27

Asare-Okai, P. N., E. Agustin, D. Fabris, and M. Royzen. "Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans-cyclooctene and tetrazine." Chem. Commun. 50, no. 58 (2014): 7844–47. http://dx.doi.org/10.1039/c4cc02435d.

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28

Sabino, Fabio, Martin Soste, Dominique Kamber, et al. "Abstract 5776: Target identification, selectivity profiling and binding site mapping of small molecule and peptide drugs by LiP-MS." Cancer Research 84, no. 6_Supplement (2024): 5776. http://dx.doi.org/10.1158/1538-7445.am2024-5776.

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Abstract Target identification in the cellular context is a critical step for both target-based and phenotypic drug discovery. Limited proteolysis coupled with mass spectrometry (LiP-MS) has emerged as a powerful technique for target deconvolution of small molecules or peptides in cell lysate without compound modification or genetic manipulation of cell lines [1,2,3,4]. Utilizing a non-specific protease under well-controlled conditions, LiP-MS exploits drug-induced structural alteration or steric hindrance on protein targets and utilizes quantitative mass spectrometry to probe over 250’000 pep

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29

Tian, Quan, Winnie Wong, Yang Xu, et al. "Immobilisation of quantum dots by bio-orthogonal PCR amplification and labelling for direct gene detection and quantitation." Chemical Communications 48, no. 44 (2012): 5467. http://dx.doi.org/10.1039/c2cc30680h.

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30

Fang, Yi, Aihua Zhang, Shaohua Li, Michael Sproviero, and Ming-Qun Xu. "Enzyme Immobilization for Solid-Phase Catalysis." Catalysts 9, no. 9 (2019): 732. http://dx.doi.org/10.3390/catal9090732.

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The covalent immobilization of an enzyme to a solid support can broaden its applicability in various workflows. Immobilized enzymes facilitate catalyst re-use, adaptability to automation or high-throughput applications and removal of the enzyme without heat inactivation or reaction purification. In this report, we demonstrate a step-by-step procedure to carry out the bio-orthogonal immobilization of DNA modifying enzymes employing the self-labelling activity of the SNAP-tag to covalently conjugate the enzyme of interest to the solid support. We also demonstrate how modifying the surface functi

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31

Jurković, Marta, Matthias Ferger, Isabela Drašković, Todd B. Marder, and Ivo Piantanida. "Triarylborane-“Click” Fluorescent Tag for Orthogonal Amino Acid Labelling, Interactions with DNA, Protein, and Cyclodextrins." Pharmaceuticals 16, no. 9 (2023): 1208. http://dx.doi.org/10.3390/ph16091208.

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The innovative design of a triarylborane (TB)-dye with one NMe2-alkylated (propargylated) group and one NMe2 group yielded a system that is both an NMe2 π-donor and an inductive NMe2-alkyl cationic acceptor. Consequently, the new TB-dye was highly sensitive to a “click” reaction with an azide-substituted lysine side chain (yielding TB-lysine), resulting in a bathochromic shift of emission of 100 nm. In addition, fluorene attached to the lysine C-terminus showed FRET with the TB-chromophore, also sensitive to interactions with targets. Both the TB-dye and TB-lysine showed high affinities toward

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32

Le, Henry H., Min-Ting Lee, Kevin R. Besler, Janine M. C. Comrie, and Elizabeth L. Johnson. "Characterization of interactions of dietary cholesterol with the murine and human gut microbiome." Nature Microbiology 7, no. 9 (2022): 1390–403. http://dx.doi.org/10.1038/s41564-022-01195-9.

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AbstractConsumption of dietary lipids, such as cholesterol, modulates the gut microbiome with consequences for host health through the production of microbiome-derived metabolites. Despite the implications for host metabolism, a limited number of specific interactions of the gut microbiome with diet-derived lipids have been characterized. This is partially because obtaining species-level resolution of the responsible taxa can be challenging and additional approaches are needed to identify health-relevant metabolites produced from cholesterol–microbiome interactions. Here we performed bio-ortho

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33

Liu, Hui, Yafen Wang, and Xiang Zhou. "Labeling and Sequencing Nucleic Acid Modifications using Bio-Orthogonal Tools." RSC Chemical Biology, 2022. http://dx.doi.org/10.1039/d2cb00087c.

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34

Liu, Yan, Michael J. Conboy, Melod Mehdipour, et al. "Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis." Nature Communications 8, no. 1 (2017). http://dx.doi.org/10.1038/s41467-017-00698-y.

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35

Liu, Yan, Michael J. Conboy, Melod Mehdipour, et al. "Addendum: Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis." Nature Communications 9, no. 1 (2018). http://dx.doi.org/10.1038/s41467-017-02779-4.

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36

Li, Shuqi, Hong-Rui Zhang, Yang Yang, et al. "Imaging‐Based High‐Content Screening with Clickable Probes Identifies XPB Inhibitors." Angewandte Chemie International Edition, July 2025. https://doi.org/10.1002/anie.202505585.

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High‐content screening (HCS) has become a powerful tool in drug discovery; however, its reliance on indirect readouts and surrogate markers limits HCS’s ability to directly assess drug‐protein interactions at endogenous levels, particularly in subcellular contexts. Here, we report an approach to address these limitations by combining confocal imaging‐based HCS and bio‐orthogonal labeling with clickable probes. As a proof‐of‐concept, we synthesized a probe TL‐alkyne (TL‐alk) that rapidly and specifically labels xeroderma pigmentosum type B (XPB), a critical protein in nucleotide excision repair

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37

Li, Shuqi, Hong-Rui Zhang, Yang Yang, et al. "Imaging‐Based High‐Content Screening with Clickable Probes Identifies XPB Inhibitors." Angewandte Chemie, July 2025. https://doi.org/10.1002/ange.202505585.

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High‐content screening (HCS) has become a powerful tool in drug discovery; however, its reliance on indirect readouts and surrogate markers limits HCS’s ability to directly assess drug‐protein interactions at endogenous levels, particularly in subcellular contexts. Here, we report an approach to address these limitations by combining confocal imaging‐based HCS and bio‐orthogonal labeling with clickable probes. As a proof‐of‐concept, we synthesized a probe TL‐alkyne (TL‐alk) that rapidly and specifically labels xeroderma pigmentosum type B (XPB), a critical protein in nucleotide excision repair

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38

Liu, Yan, Michael J. Conboy, Melod Mehdipour, et al. "Author Correction: Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis." Nature Communications 11, no. 1 (2020). http://dx.doi.org/10.1038/s41467-020-17049-z.

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39

Zhou, Lin, Lai Wang, Xue Song, Xinfu Zhang, and Yi Xiao. "A Tetrazine-Based Ratiometric Sensor Quantifying pH Gradient in Tumorspheres through Bio-Orthogonal Labeling." Analytical Chemistry, May 29, 2025. https://doi.org/10.1021/acs.analchem.5c00291.

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40

Søgaard, Ane Bretschneider, Rikke Fabech Hansson, Anne Selch Tvilum, and Alexander N. Zelikin. "Artificial Internalizing Receptors: Intracellular Delivery of Cargo Through Bio‐Orthogonal Recognition." Advanced Healthcare Materials, October 21, 2024. http://dx.doi.org/10.1002/adhm.202402472.

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AbstractDrug targeting is a methodology that helps to overcome the side effects of therapeutic molecules. However, insufficient targeting specificity and the on‐target/off‐site delivery leave much room for improvement in the targeting endeavors. One approach to enhance the specificity of drug targeting is to engineer artificial receptors with recognition ligands not observed in nature. To this end, artificial internalizing receptors that feature cholesterylamine as the artificial pull‐in mechanism, and an anti‐fluorescein antibody as the exofacial recognition and capture tool are developed. Fl

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41

Qu, Yangyang, François-Xavier Sauvage, Gilles Clavier, Fabien Miomandre, and Pierre Audebert. "Metal-Free Synthetic Approach to 3-Monosubstituted Unsymmetrical 1,2,4,5-Tetrazines Useful for Bio-orthogonal Reactions." July 17, 2018. https://doi.org/10.1002/anie.201804878.

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A facile, efficient and metal-free synthetic approach to 3-monosubstituted unsymmetrical 1,2,4,5-tetrazines is presented. Dichloromethane (DCM) is for the first time recognized as a novel reagent in the synthetic chemistry of tetrazines. Eleven 3-aryl/alkyl 1,2,4,5-tetrazines were prepared in excellent yields (up to 75%) through this novel approach. The mechanism of this new reaction, including the role of DCM in the tetrazine ring formation, has been investigated by DCM<sup>13</sup>C labeling, and is also presented and discussed as well as the photophysical and electrochemical properties.

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42

James, Leo C. "Genetic Code Expansion and Bio-Orthogonal Labeling Reveal Intact HIV-1 Capsids inside the Nucleus." mBio, September 13, 2022. http://dx.doi.org/10.1128/mbio.02346-22.

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Microscopy is one of the few techniques that can directly observe the HIV-1 capsid as it traverses the cell. However, an extrinsic or intrinsic label is needed to facilitate detection and this can perturb capsid behavior.

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43

Williams, Caroline, Bella R. Carnahan, Stephen N. Hyland, Kristen E. DeMeester, and Catherine L. Grimes. "Bio-orthogonal Labeling of Chitin in Native Pathogenic Candida Species via the Chitin Scavenge Pathway." Journal of the American Chemical Society, February 9, 2025. https://doi.org/10.1021/jacs.4c11554.

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44

Ghosh, Souvik, Ramona Birke, Ashwin Karthick Natarajan, and Johannes Broichhagen. "Penta‐ALFA‐Tagged Substrates for Self‐Labelling Tags Allow Signal Enhancement in Microscopy." Journal of Peptide Science 31, no. 5 (2025). https://doi.org/10.1002/psc.70015.

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ABSTRACTSelf‐labelling proteins like SNAP‐ and HaloTag have advanced imaging in life sciences by enabling live‐cell labeling with fluorophore‐conjugated substrates. However, the typical one‐fluorophore‐per‐protein system limits signal intensity. To address this, we developed a strategy using the ALFA‐tag system, a 13‐amino acid peptide recognized by a bio‐orthogonal and fluorescently labelled nanobody, for signal amplification. We synthesized a pentavalent ALFA5 peptide and used an azidolysine for conjugation with a Cy5‐modified SNAP‐ or HaloTag ligand through strain‐promoted click chemistry.

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45

van Dalen, Floris J., Thomas Bakkum, Tyrza van Leeuwen, et al. "Application of a Highly Selective Cathepsin S Two-step Activity-Based Probe in Multicolor Bio-Orthogonal Correlative Light-Electron Microscopy." Frontiers in Chemistry 8 (February 8, 2021). http://dx.doi.org/10.3389/fchem.2020.628433.

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Cathepsin S is a lysosomal cysteine protease highly expressed in immune cells such as dendritic cells, B cells and macrophages. Its functions include extracellular matrix breakdown and cleavage of cell adhesion molecules to facilitate immune cell motility, as well as cleavage of the invariant chain during maturation of major histocompatibility complex II. The identification of these diverse specific functions has brought the challenge of delineating cathepsin S activity with great spatial precision, relative to related enzymes and substrates. Here, the development of a potent and highly select

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46

Yao, Yunyan, Ru Jia, Chuanming Liu, et al. "An in‐situ‐tag‐generation proximity labeling technology for recording cellular interactions." Angewandte Chemie, May 3, 2024. http://dx.doi.org/10.1002/ange.202407109.

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Obtaining information about cellular interactions is fundamental to the elucidation of physiological and pathological processes. Proximity labeling technologies have been widely used to report cellular interactions in situ; however, the reliance on addition of tag molecules typically restricts their application to regions where tags can readily diffuse, while the application in, for example, solid tissues, is susceptible. Here, we propose an "in‐situ‐tag‐generation mechanism" and develop the GalTag technology based on galactose oxidase (GAO) for recording cellular interactions within three‐dim

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47

Yao, Yunyan, Ru Jia, Chuanming Liu, et al. "An in‐situ‐tag‐generation proximity labeling technology for recording cellular interactions." Angewandte Chemie International Edition, May 3, 2024. http://dx.doi.org/10.1002/anie.202407109.

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Obtaining information about cellular interactions is fundamental to the elucidation of physiological and pathological processes. Proximity labeling technologies have been widely used to report cellular interactions in situ; however, the reliance on addition of tag molecules typically restricts their application to regions where tags can readily diffuse, while the application in, for example, solid tissues, is susceptible. Here, we propose an "in‐situ‐tag‐generation mechanism" and develop the GalTag technology based on galactose oxidase (GAO) for recording cellular interactions within three‐dim

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48

Liu, Han-Hsuan, Daniel B. McClatchy, Lucio Schiapparelli, Wanhua Shen, John R. Yates, and Hollis T. Cline. "Role of the visual experience-dependent nascent proteome in neuronal plasticity." eLife 7 (February 7, 2018). http://dx.doi.org/10.7554/elife.33420.

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Experience-dependent synaptic plasticity refines brain circuits during development. To identify novel protein synthesis-dependent mechanisms contributing to experience-dependent plasticity, we conducted a quantitative proteomic screen of the nascent proteome in response to visual experience in Xenopus optic tectum using bio-orthogonal metabolic labeling (BONCAT). We identified 83 differentially synthesized candidate plasticity proteins (CPPs). The CPPs form strongly interconnected networks and are annotated to a variety of biological functions, including RNA splicing, protein translation, and

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49

Peters, Simon, Lena Kaiser, Julian Fink, et al. "Click-correlative light and electron microscopy (click-AT-CLEM) for imaging and tracking azido-functionalized sphingolipids in bacteria." Scientific Reports 11, no. 1 (2021). http://dx.doi.org/10.1038/s41598-021-83813-w.

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AbstractSphingolipids, including ceramides, are a diverse group of structurally related lipids composed of a sphingoid base backbone coupled to a fatty acid side chain and modified terminal hydroxyl group. Recently, it has been shown that sphingolipids show antimicrobial activity against a broad range of pathogenic microorganisms. The antimicrobial mechanism, however, remains so far elusive. Here, we introduce ‘click-AT-CLEM’, a labeling technique for correlated light and electron microscopy (CLEM) based on the super-resolution array tomography (srAT) approach and bio-orthogonal click chemistr

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50

Fan, Jinghao, Fang Wang, Shiyi Chen, et al. "Boosting Immune Checkpoint Therapy Efficacy of Pancreatic Cancer by Cell‐Anchoring Probiotic." Advanced Functional Materials, June 19, 2025. https://doi.org/10.1002/adfm.202502197.

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AbstractLive bacteria in tumor sites can recruit and activate T cells, enhancing the efficacy of immune checkpoint therapy (ICT) for cancer. However, the low bacterial presence within tumors may insufficiently trigger ICT. Here bio‐orthogonal click chemistry is utilized to develop cell‐anchoring probiotic that significantly improves bacterial colonization and boosts efficacy of ICT in pancreatic ductal adenocarcinoma (PDAC). The strategy involves metabolically labeling pancreatic cancer cells with azide groups both in vitro and in vivo, facilitating the covalent anchoring of dibenzocyclooctyne

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