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Mudi, La, Andi Bahrun, and Gusti Ayu Kade Sutariati. "Bio-Priming Benih Menggunakan Campuran Rizobakter Indigenous untuk meningkatkan Kualitas Fisiologis Benih Kedelai (Glycine max L. Merril)." Berkala Penelitian Agronomi 5, no. 2 (2019): 1. http://dx.doi.org/10.33772/bpa.v6i1.7508.
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Penelitian ini bertujuan untuk mempelajari efektivitas bio-priming benih menggunakan campuaran rizobakter indigenous untuk meningkatkan kualitas fisiologis benih kedelai. Penelitian telah dilakukan pada Bulan November 2014 sampai dengan Januari 2015 di Laboratorium Agroteknologi Fakultas Pertanian Universitas Halu Oleo. Penelitian ini dilaksanakan menggunakan Rancangan Acak Lengkap (RAL) factor tunggal yang terdiri dari delapan perlakuan yaitu: control (tanpa perlakuan rizobakter), bio-priming menggunakan Bacillus sp. CKD061, bio-priming menggunakan P. fluorescens PG01, bio-priming menggunakan Serratia sp. CMN175, bio-priming menggunakan campuran Bacillus sp. CKD061 + P. fluorescens PG01, bio-priming menggunakan campuran Bacillus sp. CKD061 + Serratia sp. CMN175, bio-priming menggunakan campuran P. fluorescens PG01 + Serratia sp. CMN175 dan bio-priming menggunakan campuran Bacillus sp. CKD061 + P. fluorescens PG01 + Serratia sp. CMN175. Setiap perlakuan diulang sebanyak tiga kali sehingga seluruhnya terdiri dari 24 unit percobaan. Data hasil penelitian dianalisis ragam dengan uji lanjut Duncan’s Multiple Range Test (DMRT). Hasil pengamatan pengujian fisiologis benih menunjukkan bahwa bio-priming menggunakan campuran Bacillus sp. CKD061 + P. fluorescens PG01 meningkatkan kualitas fisiologis benih. Bio-priming benih menggunakan campuran Bacillus sp. CKD061 + P. fluorescens PG01 memberikan hasil yang lebih baik dalam meningkatkan viabilitas dan vigor benih kedelai.Keywords: Bacillus sp. CKD061 + P. fluorescens PG01, bio-priming benih, campuran rizobakter, kualitas fisiologis benih, rizobakter Indigenous,
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Kumar, Gopal, Amit Kumar Pradhan, Kasturikasen Beura, Chitrangda Parihar, and Saurabh Kumar Choudhary. "Influence of Chelated Fe and Zn Co Application on Soil Physicochemical Characteristics during Lentil Developmental Stages." Journal of Scientific Research and Reports 31, no. 6 (2025): 879–87. https://doi.org/10.9734/jsrr/2025/v31i63181.
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Lentil is a key pulse crop in developing nations, yet its productivity and nutritional value are often limited by micronutrient deficiencies, particularly iron (Fe) and zinc (Zn). A randomized block design with 10 treatments under a rice system including RDF (Recommended dose fertilizer) alone (T10), and combinations of iron (Fe) or zinc (Zn) or both applied through basal application, bio-priming, nutripriming, and foliar spray: T1–Fe (basal + foliar), T2–Fe (bio-priming + nutripriming), T3–Fe (basal + bio-priming + nutripriming), T4–Zn (basal + foliar), T5–Zn (bio-priming + nutripriming), T6–Zn (basal + bio-priming + nutripriming), T7–Fe+Zn (basal + foliar), T8–Fe+Zn (bio-priming + nutripriming), and T9–Fe+Zn (basal + bio-priming + nutripriming). This study assessed the effects of chelated iron (Fe) and zinc (Zn) applied through basal application, nutripriming, and biopriming on soil properties and micronutrient dynamics in lentil cultivation. Treatments moderately influenced soil pH, EC, oxidizable carbon, CEC, and macronutrient levels. The highest nutrient enrichment was seen in T3 and T9, which also significantly improved Fe (29.72 mg kg⁻¹ at tillering) and Zn (1.20 mg kg⁻¹ at flowering), respectively. DTPA-extractable Fe and Zn declined from vegetative to post-harvest stages. The RDF control (T10) showed the lowest micronutrient levels. Integrated Fe and Zn application effectively improved soil fertility and supported biofortification.
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Choudhury, Anish, Sayandeep Sutradhar, and Sanjoy Kumar Bordolui. "Impact of bio-priming (Rhizobium leguminosarum ) to improve seedling vigour and germination potential to overcome abiotic stress in green gram." Ecology, Environment and Conservation 30, SUPPL (2024): S42—S46. http://dx.doi.org/10.53550/eec.2024.v30i03s.009.
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Diverse seed priming methods are used to ameliorate seed germination, seedling vigour, and to overcome abiotic stress. Insertion to these, only the bio-priming system provides the fresh benefit of biotic stress operation, earning it special attention. Bio-priming is applicable in nearly all crops around the world and is an environmentally friendly volition to chemical pesticides. Seed bio-priming generally refers to use of salutary microorganisms to influence germination potential. In this study, Rhizobium leguminosarum were used in different concentration following Complete Randomized Design with three replications. Green gram (TMB-37) was collected from AICRP pulses in BCKV, Mohanpur, Nadia, West Bengal for this investigation. The experiment comprised of seven bio-priming treatments along with control. The seeds were subjected to in-vitro studies and data were analysed statistically. Seed bio-primed with Rhizobium leguminosarum @ 20 percent recorded higher seed germination percentage (82.71 %), shoot length (25.49 cm), seedling fresh weight (2.77 g), seedling dry weight (0.24 g), seedling vigour index I (3467.91) and seedling vigour index II (19.85). The results of this study demonstrated that Rhizobium leguminosarum @ 20 percent was the best.
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Phooi, Chooi Lin, Elisa Azura Azman, and Roslan Ismail. "Effect of Priming on Brassica rapa subsp. chinensis (Bok Choy) Seeds Germination." agriTECH 43, no. 4 (2023): 288. http://dx.doi.org/10.22146/agritech.74856.
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Brassica rapa subsp. Chinensis, commonly known as Bok Choy, is a nutrient-rich vegetable with substantial antioxidant content. Therefore, this study aimed to evaluate the effect of hydropriming and bio-nutri-priming using Sandwich compost leachate on seed germination, SPAD reading, and dry matter accumulation in 280 dwarf variants of Bok Choy seeds sourced from Green Eagle. The experimental process involved hydropriming with tap water, bio-nutri-priming using 0.2% Sandwich compost leachate, and a control group cultivated in soil without priming. A complete randomization design (CRD) with three replications assessed seed germination performance, SPAD, root and shoot dry matter, and root-to-shoot ratio. While there was no significant difference in the germination percentage (88.35±1.13%), the entire priming seeds exhibited a 2-day peak germination period, compared to 3 days for the non-priming counterparts. Bio-nutri-priming seeds showed faster median and mean germination times due to enhanced nutrient uptake. They further displayed high SPAD readings, suggesting a lack of toxic compounds. The dry matter production of all treated Bok Choy was similar because administered treatments did not interfere with plant growth and development. Therefore, applying bio-nutri-priming using Sandwich compost leachate positively affected seed germination performance, warranting its recommendation as a seeds priming solution.
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Phooi, Chooi Lin, Elisa AzmanAzura, Roslan Ismail, and Shafeeqa Shahruddin. "Effect of Sandwich Compost Leachate on Allium tuberosum Seed Germination." Pertanika Journal of Tropical Agricultural Science 45, no. 2 (2022): 481–90. http://dx.doi.org/10.47836/pjtas.45.2.09.
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Food waste is a serious global issue, and one way to reduce the impact of food waste is by composting. Sandwich compost is a type of fermented food waste compost created with microbial fermentation; meanwhile, the composting leachate provides nutrients for plants. Studies have shown that seed germination may be enhanced when treated with sandwich compost leachate. Furthermore, few studies have been on sandwich compost leachate used for seed priming. The objective of this study was to determine the effect of varying leachate concentrations of food waste sandwich compost and priming durations on the performance of Chinese chive (Allium tuberosum) seed germination. Chinese chive (Allium tuberosum) was chosen as the test crop. It is widely used as a flavouring herb with high economic potential; however, its seed germination time is long and requires pre-treatment such as crushing and seed priming to speed up the germination process. The study used four replications and a complete randomisation design (CRD). The seeds were exposed to different percentages of sandwich compost leachate (0.0%, 0.2%, 0.4%, 0.6%, 0.8%, and 1.0%) and priming duration (4, 8, and 12 hours). A significant interaction between the bio-nutri-priming concentration and priming duration was demonstrated by measuring the standard error of germination rate () and corrected germination rate index (Scorrected). A longer bio-nutri-priming duration was key for a higher seed vigour index. The bio-nutri- priming concentration and priming duration, however, had no significant interaction. Longer bio-nutri-priming durations were recommended to obtain better germination performance of Chinese chive. The study showed that a twelve-hour bio-nutri-priming duration and a 0.6 % leachate concentration significantly enhanced the Chinese chive seed germination and helped break seed dormancy.
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Callan, Nancy W., James B. Miller, and Don E. Mathre. "FIELD PERFORMANCE OF BIO-PRIMING FOR PROTECTION OF sh2 SWEET CORN FROM PREEMERGENCE DAMPING-OFF CAUSED BY PYTHIUM ULTIMUM." HortScience 25, no. 9 (1990): 1110f—1110. http://dx.doi.org/10.21273/hortsci.25.9.1110f.
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Shrunken-2 supersweet (sh2) sweet corn is susceptible to preemergence damping-off caused by Pythium ultimum, especially when planted into cold soil. Bio-priming, a seed treatment which combines the establishment of a bioprotectant on the seed with preplant seed hydration, was developed to protect seeds from damping-off.In a series of field experiments conducted in Montana's Bitterroot and Gallatin Valleys, bio-priming or seed bacterization with Pseudomonas fluorescens AB254 protected sweet corn from P. ultimum damping-off. Bio-priming corn seed with P. fluorescens AB254 was comparable to treatment with the fungicide metalaxyl in increasing seedling emergence. Seedlings from bio-primed seeds emerged from the soil more rapidly than from nontreated seeds and were larger at three weeks postplanting. Seeds of sh 2 and sugary enhancer (se) sweet corn, as well as that of several sh 2 cultivars, were protected from damping-off by bio-priming.
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NARAYANAN, G. SATHIYA, S. ARUNKUMAR, D. VENKATAKRISHNAN, and D. S. BALAJI. "The implications of various seed priming treatments on seed yield in proso millet (Panicum miliaceum)." Indian Journal of Agricultural Sciences 94, no. 8 (2024): 833–40. http://dx.doi.org/10.56093/ijas.v94i8.147724.
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The experiment was conducted during rainy (kharif) season of 2021 and winter (rabi) season of 2022 at Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu to identify suitable seed priming technique, soaking duration and priming agents for halo, osmo, and bio-priming in proso millet (Panicum miliaceum L.). To increase crop yield and sustainability in present-day agriculture, seed priming continues to be essential by utilizing scientific knowledge and technical breakthroughs. The experiment was laid out in factorial completely randomized design (FCRD) with three replications. The fresh seeds of proso millet cv. CO4 were imposed for halo, osmo, and bio priming treatments, viz. Agent for halo-priming: Control, hydropriming, KH2PO4 (1 and 2%), ZnSO4 (1 and 2%), and CaCl2 (1 and 2%); Agent for osmo-priming: Control, hydropriming, NaCl (1 and 2%), Mannitol (1 and 2%), and PEG (-10 and -15 Bar); Agent for bio-priming: Control, hydropriming, Azospirillum (15 and 20%), P. fluorescens (LF) (10 and 15%), Prosophis LE (5 and 10%), and soaked in equal volumes for 6 and 8 h. The results revealed that for halo priming, seeds soaked in 2% KH2PO4 for 8 h; for osmo priming seeds soaked in 2% mannitol for 8 h and for bio-priming seeds soaked in 15% P. fluorescens (LF) for 8 h outperformed other treatments. Field experiments were also conducted to determine the productivity of seed primed proso millet. The results showed that the crop performance was superior in seeds primed with 2% KH2PO4 for 8 h during kharif season than rabi.
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Kumar, Gopal, Amit Kumar Pradhan, Kasturikasen Beura, Chitrangda Parihar, and Saurabh Kumar Choudhary. "Impact of Chelated Iron and Zinc Application Methods on Soil Properties and Micronutrient Availability during Rice Growth Stages." International Journal of Plant & Soil Science 37, no. 6 (2025): 561–70. https://doi.org/10.9734/ijpss/2025/v37i65535.
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Zn and Fe are the most limiting nutrients for plant development and metabolism. This study evaluated the impact of Fe and Zn supplementation alternate methods such as biopriming, nutripriming, foliar and basal application. A randomized block design with 10 treatments under a rice system including RDF alone (T10), and combinations of iron (Fe) or zinc (Zn) or both applied through basal application, bio-priming, nutripriming, and foliar spray: T1–Fe (basal + foliar), T2–Fe (bio-priming + nutripriming), T3–Fe (basal + bio-priming + nutripriming), T4–Zn (basal + foliar), T5–Zn (bio-priming + nutripriming), T6–Zn (basal + bio-priming + nutripriming), T7–Fe+Zn (basal + foliar), T8–Fe+Zn (bio-priming + nutripriming), and T9–Fe+Zn (basal + bio-priming + nutripriming).This study evaluated the influence of different iron (Fe) and zinc (Zn) management strategies on soil chemical properties and the availability of DTPA-extractable Fe and Zn at critical growth stages of rice under a rice-based cropping system. Treatments involved sole and combined applications of Fe and Zn via basal, foliar, biopriming, and nutripriming methods. Soil chemical properties, including pH, electrical conductivity (ECe), oxidizable carbon, cation exchange capacity (CEC), and macronutrients (N, P, K), showed no statistically significant variation across treatments, although numerical differences were observed. The combined application of Fe and Zn (T9: basal + nutripriming + biopriming) consistently resulted in the highest DTPA-Fe and Zn concentrations across tillering, flowering, and post-harvest stages. DTPA-Fe ranged from 20.46 to 29.99 mg kg⁻¹ at tillering, while DTPA-Zn ranged from 0.77 to 1.47 mg kg⁻¹. A general declining trend was observed for both nutrients as the crop progressed. Results affirm that integrated micronutrient application enhances soil Fe and Zn bioavailability, demonstrating the potential of agronomic biofortification strategies to address micronutrient deficiencies in rice-based systems.
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Gantait, Aishmita, Sam A. Masih, and Ann Maxton. "Effect of Biological Priming on Metabolomic and Molecular Changes in Response to Drought Stress in Brassica juncea." Journal of Advances in Biology & Biotechnology 27, no. 8 (2024): 1325–38. http://dx.doi.org/10.9734/jabb/2024/v27i81256.
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Indian mustard, Brassica juncea is a leading oilseed crop in India and plays a crucial role in the agricultural as well as oilseed home marketing system of the country. High oil and phytosterols, glucosinolate content make it a more valuable crop, and it is used for edible oil production, poultry feed. There, however, is always one major problem during the growth of trees and their productivity; drought. This paper discusses the use of bio-priming to compound drought resistance in Indian mustard. Bio-priming, which entails the use of various microorganisms in seed treatment is therefore modern system of farming. In the process of germination and plant growth it enhances the seed germination rate, seedling vigor and overall plant health through various metabolomic and molecular mechanisms. Researchers have demonstrated that bio-priming containing elements such as Pseudomonas fluorescens, Bacillus subtilis, and Trichoderma harzianum faciliates the uptake of sulfur, growth and improvement of tolerance to abiotic stresses. Furthermore, by impacting on expression of stress associated genes, bio-priming enhances the activity of the WRKY transcription factor as well as the production of abscisic acid (ABA) and jasmonic acid (JA), leading to drought tolerance. These first results based on drought tolerance genes help to priorities future breeding programs dedicated to the improvement of drought resistant cultivars. Bio-priming also stimulated the antioxidant defense pathway and improved the ability of plants to cope with oxidation pressure arising from drought stress. Overall, bio-priming is a relatively cost-effective, environmentally considerate method for enhancing drought tolerance in Indian mustard, thus being beneficial for sustainable farming and food production.
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Stałanowska, Karolina, Viorica Railean, Paweł Pomastowski, Agnieszka Pszczółkowska, Adam Okorski, and Lesław Bernard Lahuta. "Seeds Priming with Bio-Silver Nanoparticles Protects Pea (Pisum sativum L.) Seedlings Against Selected Fungal Pathogens." International Journal of Molecular Sciences 25, no. 21 (2024): 11402. http://dx.doi.org/10.3390/ijms252111402.
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Nano-priming is a relatively new seed treatment technique using metal and metal oxide nanoparticles (NPs), and such application of NPs may support the plants’ immunity. Recently we have shown that the that biologically synthesized silver nanoparticles (bio-AgNPs) used as short-term foliar treatment protect pea seedlings against D. pinodes and F. avenaceum. In the present study, the protection of peas against both fungal pathogens via seed priming with bio-AgNPs was analyzed. Moreover, the changes in the polar metabolic profiles of the seedlings caused by priming and infection were also compared. Seed priming with bio-AgNPs at concentrations of 50 and 100 mg/L considerably reduced the symptoms and infection levels of both pathogens by over 70% and 90% for F. avenaceum and D. pinodes, respectively. Pathogens infection and nano-priming affected the metabolic profile of pea seedlings. The major changes in the primary metabolism were observed among carbohydrates and amino acids. In turn, this may result in changes in the expression and accumulation of secondary metabolites. Therefore, further investigation of the effect of nano-priming should focus on the changes in the secondary metabolism.
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P, Geetharani, C. Parameswari, M. P. Kavitha, G. Sudhakar, and M. Madhan Mohan. "Bio-Stimulants Humid Priming: A Technology for Improving Seed Vigour of Annual Moringa cv. PKM 1." Journal of Experimental Agriculture International 47, no. 1 (2025): 1–5. https://doi.org/10.9734/jeai/2025/v47i13187.
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Moringa oleifera Lam. is a multiple purpose tree used as human and animal food, cosmetic production and water purification. Seed priming combined with organic bostimulants has been used to improve plant performance. This study aimed to verify the efficiency of humid seed priming using bio stimulants such as jeevamirtham, panchagavya, moringa leaf extract, seaweed extract, egg amino acid and liquid-based bio formulations of Bacillus subtilis on seed germination and seedling growth of Moringa oleifera. The humid priming technique involves soaking seeds in a loosely tied cloth bag for a predetermined duration, then placing the bags in a closed container on an elevated platform to provide dark, humid conditions that facilitate the invigoration process. Seeds are soaked for 12 hours, exposed to dark, humid conditions for another 12 hours, and then shade dried to the original moisture content for proper handling. The experiment on seed invigoration with humid priming was conducted at Agricultural Research Station, Vaigai Dam, during 2023 with annual moringa variety PKM1. The annual moringa seeds were subjected to humid priming with various bio-stimulants in different concentrations to determine the seed quality parameters viz., speed of germination, germination percentage, seedling length, dry weight and seedling vigour. All the humid priming treatments with bio stimulants increased the emergence rate and enhanced the seedling vigor. However, humid priming with 1% liquid formulations of Bacillus subtilis had a significantly positive influence on speed of germination (6.64), days for 50% emergence (4.5 days), maximum germination percentage (94%), root length (15.7 cm), shoot length (29.2 cm), dry matter production (3.6 g/10 seedlings), and vigour index value (4220) over the control which recorded only 5.14,8.0,74 %,9.3cm, 18.9cm,2.05g, 2086 respectively. The germination percentage increased by 27% in humid priming with 1% Bacillus subtilis compared to control.
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El-Hamed, Khalid, Mohammed Elwan, and Walied Shaban. "Enhanced Sweet Corn Propagation: Studies on Transplanting Feasibility and Seed Priming." Vegetable Crops Research Bulletin 75, no. 1 (2011): 31–50. http://dx.doi.org/10.2478/v10032-011-0016-4.
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Enhanced Sweet Corn Propagation: Studies on Transplanting Feasibility and Seed PrimingSweet corn hybrids with high-sugar genotypes (sh2) has inherent problem of low seed emergence and stand in the field. This study was conducted to determine the effect of seed size, tray cell size and growing media components on sweet corn transplant transplanting. Other objectives were to evaluate the effect of priming sweet corn seeds on germination in the field. Bio-priming withTrichodermaandBacillus, osmopriming with KNO3, and hydro-priming with H2O have been tested. The results indicated that transplanting sweet corn is feasible with high quality transplants from seeds that germinate well in disease-free environment. Large sweet corn seeds, large tray cells, and vermiculite-based growing media proved to gave higher germination percentages. While same factors did not show pronounced effect on seedling performance in terms of root and shoot length and fresh weight. In the priming experiment, the bio-priming treatment showed the highest germination of seeds percentage among other priming treatments and the control. Sweet corn seeds treated withBacillus megateriumgerminated 50% higher than seeds treated withTrichodermaspp. as bio-control agents.Aspergillus niger, andPenicilliumrepresented 65% of pathogens responsible for failure of sweet corn seed germination. The results of this study demonstrated the feasibility of enhanced sweet corn seed propagation through transplanting and seed priming to improve emergence and field stand.
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Songsaeng, Apisit, Pakpoom Boonchuen, Phongkeat Nareephot, et al. "Enhancing Resistance to Cercospora Leaf Spot in Mung Bean (Vigna radiata L.) through Bradyrhizobium sp. DOA9 Priming: Molecular Insights and Bio-Priming Potential." Plants 13, no. 17 (2024): 2495. http://dx.doi.org/10.3390/plants13172495.
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Mung bean (Vigna radiata L.), a vital legume in Asia with significant nutritional benefits, is highly susceptible to Cercospora leaf spot (CLS) caused by Cercospora canescens, leading to significant yield losses. As an alternative to chemical fungicides, bio-priming with rhizobacteria can enhance plant resistance. This study explores the potential of Bradyrhizobium sp. strain DOA9 to augment resistance in mung bean against CLS via root priming. The results reveal that short (3 days) and double (17 and 3 days) priming with DOA9 before fungal infection considerably reduces lesion size on infected leaves by activating defense-related genes, including Pti1, Pti6, EDS1, NDR1, PR-1, PR-2, Prx, and CHS, or by suppressing the inhibition of PR-5 and enhancing peroxidase (POD) activity in leaves. Interestingly, the Type 3 secretion system (T3SS) of DOA9 may play a role in establishing resistance in V. radiata CN72. These findings suggest that DOA9 primes V. radiata CN72′s defense mechanisms, offering an effective bio-priming strategy to alleviate CLS. Hence, our insights propose the potential use of DOA9 as a bio-priming agent to manage CLS in V. radiata CN72, providing a sustainable alternative to chemical fungicide applications.
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Callan, Nancy W., Don E. Mathre, and James B. Miller. "Field Performance of Sweet Corn Seed Bio-primed and Coated with Pseudomonas fluorescens AB254." HortScience 26, no. 9 (1991): 1163–65. http://dx.doi.org/10.21273/hortsci.26.9.1163.
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In field experiments, bio-priming and coating with Pseudomonas fluorescens AB254 consistently protected sweet corn (Zea mays L.) seeds from preemergence damping-off caused by Pythium ultimum Trow. The bio-priming seed treatment was evaluated under various disease pressures and with seeds of three sweet corn genotypes: shrunken-2 supersweet (sh-2), sugary enhancer (se), and sugary (su). While no damping-off occurred in the su sweet corn, bio-priming protected sh-2 and se sweet corn seeds at a level equivalent to that obtained by treatment with the fungicide metalaxyl. Biopriming increased seedling height of all three sweet corn genotypes at 4 weeks post-planting. Coating of sweet corn seeds with P. fluorescens AB254 provided an equivalent degree of protection from damping-off under all but the most severe conditions.
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Iwai, Ranko, Shunta Uchida, Sayaka Yamaguchi, et al. "Effects of Seed Bio-Priming by Purple Non-Sulfur Bacteria (PNSB) on the Root Development of Rice." Microorganisms 10, no. 11 (2022): 2197. http://dx.doi.org/10.3390/microorganisms10112197.
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The effects of seed bio-priming (seed soaking) with purple non-sulfur bacteria (PNSB) on the grain productivity and root development of rice were examined by a field study and laboratory experiments, respectively. Two PNSB strains, Rhodopseudomonas sp. Tsuru2 and Rhodobacter sp. Tsuru3, isolated from the paddy field of the study site were used for seed bio-priming. For seed bio-priming in the field study, the rice seeds were soaked for 1 day in water containing a 1 × 105 colony forming unit (cfu)/mL of PNSB cells, and the rice grain productivities at the harvest time were 420, 462 and 504 kg/are for the control, strain Tsuru2-primed, and strain Tsuru3-primed seeds, respectively. The effects of seed priming on the root development were examined with cell pot cultivation experiments for 2 weeks. The total root length, root surface area, number of tips and forks were evaluated with WinRhizo, an image analysis system, and strains Tsuru2- and Tsuru3-primed seeds showed better root development than the control seeds. The effects of seed priming with the dead (killed) PNSB cells were also examined, and the seed priming with the dead cells was also effective, indicating that the effects were attributed to some cellular components. We expected the lipopolysaccharide (LPS) of PNSB as the effective component of PNSB and found that seed priming with LPS of Rhodobacter sphaeroides NBRC 12203 (type culture) at the concentrations of 5 ng/mL and 50 ng/mL enhanced the root development.
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Rai, Anuj Kumar, Himangshu Das, and Asit Kumar Basu. "Response of Bio-priming in okra for vegetable production." Journal of Applied and Natural Science 11, no. 3 (2019): 687–93. http://dx.doi.org/10.31018/jans.v11i3.2147.
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The field experiment was conducted at District Seed Farm, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia, West Bengal in summer season of 2011 and 2012. Pre-sowing bio-priming was done with Trichoderma viride and Pseudomonas fluorescens with an un-primed control to assess the trend of okra varieties viz., Lalu, Arka Anamika, Ramya, Satsira, Lady Luck,Debpusa Jhar,Japani Jhar and Barsha Laxmi due to bio-priming of seeds towards vegetable production Significant variation among the varieties was noted for all the characters studied. Okra variety Lalu gave highest vegetable yield per plant in both years and it was statistically at par with Arka Anamika. Vegetable yield per plant was increased by 4.33 to 20.08% in first year and 3.68 to 19.60% in second year with T. viride as compared to P. fluorescens and un-primed control. Individual varieties indicated that vegetable yield per plant was maximum with Lalu when priming was made with both the bio-inoculants followed by Arka Anamika during both years. Hence, Lalu and Arka Anamika may be recommended for experimental region for higher yield and pre-sowing seed bio-priming may be recommended with both T.viride and P. fluorescens for enhanced vegetable yield of okra.
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Subrahmaniyan, K., T. Parthipan, M. Rajavel, and P. Veeramani. "Impact of conservation tillage practices in rice-blackgram cropping system." Bangladesh Journal of Botany 53, no. 3 (2024): 519–25. http://dx.doi.org/10.3329/bjb.v53i3.76267.
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Field experiments were conducted with two tillage practices in rice (Puddled and Non- puddled) during rainy season and three seed priming methods (Bio- seed priming with Rhizobium, hydropriming and no seed priming) and two foliar spray (2% DAP and 2% urea) during post rainy seasons in blackgram. The results indicated that the soil penetration resistance was found to be lesser in the soils of non-puddled tillage. The rice yield reduction due to NPTR was non-significant (less than 3 per cent). However, the soil penetration resistance was higher with the soils of blackgram sown as succeeding crop in the PTR. The blackgram yield obtained under NPTR was 10.6 per cent higher than PTR system. Bio seed priming with Rhizobium increased the yield by 26.0 per cent over no seed priming. The study indicated that NPTR showed a positive impact on the yield in the rice-blackgram relay cropping system. Bangladesh J. Bot. 53(3): 519-525, 2024 (September)
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Rashi and Nutan Kaushik. "Enhancing germination percentage and seed vigor in horticultural Crops through biopriming techniques." BIO Web of Conferences 110 (2024): 01012. http://dx.doi.org/10.1051/bioconf/202411001012.
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Seed biopriming induces a physiological condition that promotes germination and uniform seedling emergence. An investigation is carried out on different seeds such as carrot (Daucus carota subsp. Sativus), onion (Allium cepa L.), cauliflower (Brassica oleracea), radish, (Raphanus sativus), tomato (Solanum lycopersicum), spinach (Spinacia oleracea), fenugreek (Trigonella foenum-graecum), and pea (Pisum sativum) to standardise bio-priming with Bacillus siamensis strain NKIT-9 to improve seed germination and seedling vigor index. To optimise the concentration of bio-priming seedlings were bioprimed overnight with Bacillus siamensis strain (NKIT-9) at varied concentrations (0.1mg/ml, 10µg/ml, 0.1µg/ml, and 10ng/ml). Bio-priming with Bacillus siamensis strain NKIT-9 gave highest values of germination percentage and seedling vigor index of carrot (at 0.1mg/ml), onion (at 0.1µg/ml ), cauliflower at 0.1mg/ml, 10µg/ml, and 0.1µg/ml), radish (at0.1mg/ml and 0.1µg/ml), tomato (at 0.1mg/ml), spinach (at 10ng/ml ), fenugreek (at 10ng/ml) and pea (a10ng/ml ) as compared with control. carrot, onion, cauliflower, radish, tomato, spinach, fenugreek, and pea exhibited higher germination percentages (60%, 100%, 100%, 100%, 80%, 90%, 100%, and 60% respectively) and seedling vigor indexes (148 ,720, 910, 880, 525, 580, 990, and 1018 respectively) when compared with control. Therefore, bio-priming with these concentrations is best suited for improving germination rate and seedling vigor of seeds.
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MUSTAFA, HAFIZ SAAD BIN, TARIQ MAHMOOD, AHSAN ULLAH, et al. "ROLE OF SEED PRIMING TO ENHANCE GROWTH AND DEVELOPMENT OF CROP PLANTS AGAINST BIOTIC AND ABIOTIC STRESSES." Bulletin of Biological and Allied Sciences Research 2017, no. 1 (2017): 8. http://dx.doi.org/10.54112/bbasr.v2017i1.8.
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Abiotic and biotic stresses severely affect the crop’s growth and development at every stage of growth cycle. Seed priming is helpful technique to enhance seed germination and growth in stress environment. Seed priming is a control procedure followed by re-drying which will allow the seed to imbibe water stimulate the internal biological process essential for germination but this process will not permit the seed to be truly germinate. Currently many seed priming method have been used like hydro-priming, osmo-priming, halo-priming, thermo-priming, bio-priming and solid matrix priming. Various studies confirmed that seed priming has several advantages including, early emergence high water use efficiency, stand establishment, deeper roots germination in broad range of temperature and resistance against disease and environmental stresses.
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Ryavalad, Shivayogi, Ashok Surwenshi, Arvind Kumar Rathod, Muske Deepa N., Srinivas N., and Rajkumar M. "Importance of Bio-Priming on Improving Seed Quality in Chilli (Capsicum Annuum L.)." International Journal of Plant & Soil Science 35, no. 22 (2023): 519–24. http://dx.doi.org/10.9734/ijpss/2023/v35i224160.
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India grows a lot of chilli (Capsicum annuum L.), and one of its most valuable commodities is its seed. However, the quality of the seeds is lost if they are stored for the months between harvest and the subsequent sowing. The seeds of Rudra and Byadgi Dabbi varieties were subjected to bio-priming with the Pseudomonas fluorescens, Bacillus and Trichoderma viride on qualitative parameters with seven treatments. The experiment was carried out using CRBD. The culture of Pseudomonas fluorescens, Bacillus and Trichoderma viride at 2.5g /l and 5.0g/l concentration were used as pre-sowing bio-priming treatments. From the results it was found that the Bio-priming influenced significantly on the capability of the chilli seedlings with the improved germination percentage of 98.69% (2.5 g P. fluorescens, T1), shoot length 6.53cm (5 g P. fluorescens, T2), root length 7.18cm (5 g T. viride, T6), seedling length 13.43 cm (5 g P. fluorescens, T2) and seedling vigour index 1216.44 (5 g P. fluorescens, T2) in Byadgi Dabbi cultivar and where as in Rudra cultivar recorded germination percentage of 99.75% (T2), shoot length 4.13cm (5 g T. viride, T6), root length 7.38cm (5 g T. viride, T6), seedling length 11.56 cm (5 g T. viride,T6) and seedling vigour index 1140.12 (5 g T. viride, T6) when compared with control. Therefore, bio-priming with Pseudomonas fluorescens, Bacillus and Trichoderma viride are effective in enhancing the quality parameters of seed in chilli.
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Yadav, R. Ravinder Rao, S. Vasundhara, B. Rupesh Kumar Reddy, and M. Raveendra Reddy. "Effect of Bio-Priming on Seed Quality Parameters of Rice (Oryza sativa L.)." Microbiology Research Journal International 35, no. 3 (2025): 57–64. https://doi.org/10.9734/mrji/2025/v35i31550.
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This study investigates the impact of bio-priming with Azospirillum, Pseudomonas fluorescens, Phosphorus solubilizing bacteria (PSB), and Potassium solubilizing bacteria (KSB) on seed quality parameters of rice (Oryza sativa L.). Bio-priming is a sustainable agricultural practice that involves the use of beneficial microorganisms to enhance plant growth. Standardization experiment was done using 35 treatments in laboratory using Completely Randomized Design (CRD). The best performed 10 treatments were selected for field study. The field experiment was conducted in Randomized Block Design (RBD). The harvested seeds were further subjected to quality tests in the laboratory using Completely Randomized Design (CRD). Among different treatments, Azospirillum bio-primed at 10% and 12 hours of soaking and pseudomonas fluorescens bio-primed at 20% and 12 hours of soaking showed significant results for germination percentage, seedling vigour index, field emergence, highest seedling length.
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Mishra, B. K., Chetan Kumar Jangir, S. N. Saxena, Y. K. Sharma, and Krishna Kant. "Bio-priming : a precision approach to augment seed emergence and yield in Ajwain (Trachyspermum ammi)." International Journal of Seed Spices 12, no. 1 (2024): 48–53. http://dx.doi.org/10.56093/ijss.v12i1.5.
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Bio-priming, a novel seed treatment technique, has garnered attention forits capacity to enhance seed germination, crop growth, and yield. Thisstudy aimed to assess the efficacy of Trichoderma (commercial strains)and Bacillus and Pseudomonas (newly isolated strains) as sole inoculantsand co-inoculants with soaking agents during seed bio-priming to enhanceseed germination and initial seedling growth of ajwain. A randomized blockdesign (RBD) was employed, comprising seven treatments replicated-1three times each. The highest seed yield (2510.247 kgha ) and associatedattributes were observed in treatment T5 (Chitosan + Trichoderma viride)-1compared to the control (2068.51 kg ha ). Treatment T3 (Chitosan +Bacillus paramycoides + Pseudomonas aeruginosa) improved allparameters, including final germination, shoot length, root length, plantheight, primary and secondary branches, and test weight, compared to thecontrol. The correlation analysis revealed positive associations between-1yield and plant height, umbellateumbelkgha , seed emergence,-1 secondary branch, and umbelplantkgha . Consequently, Trichodermastrains followed by Bacillus paramycoides + Pseudomonas aeruginosacombined with chitosan exhibit potential as both single inoculants and co-inoculants for ajwain bio-priming, pending further validation throughfarmer field evaluations
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JANMOHAMMADI, Mohsen, Maryam MOHAMADZADEH-ALGHOO, Naser SABAGHNIA, Viorel ION, and Shahbaz NAEEM. "Seed pre-sowing treatments and essential trace elements application effects on wheat performance." Acta agriculturae Slovenica 119, no. 1 (2023): 1. http://dx.doi.org/10.14720/aas.2023.119.1.2671.
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<p class="042abstractstekst">Current study was conducted to evaluate the effects of different seed priming and foliar spray of micronutrients on bread wheat performance in semi-arid region in Northwest of Iran. Pre-sowing treatments were <em>S1: no pre-sowing treatment (intact seeds), S2: hydro-priming, S3: bio-priming (seed inoculation </em>with plant promoting rhizobacteria consortium: <em>Azotobacter chroococcum </em>+ <em>Azospirillum lipoferum</em><em>), S4: micronutrient seed priming and </em>foliar feeding <em>include, check (0): distilled water spray, Fe: foliar spray of iron, Zn: foliar spray of zinc. </em> All seed priming treatments significantly increased plant height, tiller number, canopy width, total biomass, spike mass, seed number per spike and seed yield compared to intact seeds. A brief comparison of the effect of seed priming and fertilizer treatments showed that the effects of priming treatments on improving growth and seed yield was more obvious than fertilizer treatments. The greatest increase in seed yield and yield components was recorded for plants grown from bio-fortified seeds by essential trace elements. However, comparison of fertilizer treatments showed that growth parameters were significantly affected by Zn application. From the present study, it may be concluded that combined seed priming through pre-sowing hydration, soaking in micronutrients and microbial inoculation is useful to enhance wheat production and agricultural sustainability for smallholder farmers in semi-arid region.</p>
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Callan, Nancy W., and Don E. Mathre. "Biological Seed Treatments." HortScience 30, no. 4 (1995): 749E—749. http://dx.doi.org/10.21273/hortsci.30.4.749e.
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Biological seed treatment offers a safe, environmentally responsible option for protection of seeds and seedlings from attack by soilborne pathogens. Most effective biological seed treatments have used either bacterial or fungal agents. The efficacy of a biological seed treatment depends upon the ability of the biocontrol agent to compete and function on the seed and in the rhizosphere under diverse conditions of soil pH, nutrient level, moisture, temperature, and disease pressure. Seed treatment performance may be improved through application and formulation technology. An example of this is the bio-priming seed treatment, a combination of seed priming and inoculation with Pseudomonas aureofaciens AB254, which was originally developed for protection of sh-2 sweet corn from Pythium ultimum seed decay. Bio-priming has been evaluated for protection of seed of sweet corn and other crops under a range of soil environmental conditions.
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Rai, Anuj Kumar, Himangshu Das, and Asit Kumar Basu. "Seed Quality of Okra Produced after Bio-Priming." International Journal of Current Microbiology and Applied Sciences 8, no. 06 (2019): 2166–73. http://dx.doi.org/10.20546/ijcmas.2019.806.257.
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Rawal, Janak Singh, Puspa RC, and Ashmita Mandal. "A review on seed priming to combat climate variability in agriculture." Archives of Agriculture and Environmental Science 9, no. 3 (2024): 593–605. http://dx.doi.org/10.26832/24566632.2024.0903026.
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Global agriculture faces immense challenges due to climate change, which causes unpredictable weather patterns, decreased agricultural productivity, and decreased food security. Seed priming is critical in combating climate variability because it has emerged as a promising method for improving seed germination and agricultural resilience. This review evaluates the efficiency of several seed priming techniques, including hydro-priming, halo-priming, osmo-priming, bio-priming, chemical priming, and hormone priming. These techniques improve seedling vigor, stress tolerance, and overall crop yield. Seed priming increases germination rates and resistance to biotic and abiotic stresses, such as salinity and drought, while improving agricultural output and disease resistance. Seed priming reduces the demand for chemical pesticides and fertilizers by increasing soil quality and nutrient absorption, which supports sustainable agriculture. This review highlights the potential benefits of seed priming as a practical, affordable, and practical strategy to reduce the negative effects of climatic variability on agriculture. Future studies should focus on developing the best priming techniques for diverse crop varieties and conditions, as well as examining the combined impacts of various priming strategies. Seed priming will be crucial to preserving food security and agricultural sustainability in the face of ongoing climate change.
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Miljaković, Dragana, Jelena Marinković, Gordana Tamindžić, et al. "Bio-priming of soybean with Bradyrhizobium japonicum and Bacillus megaterium: Strategy to improve seed germination and the initial seedling growth." Plants 11, no. 15 (2022): 1927. https://doi.org/10.3390/plants11151927.
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Deshmukh, Amol J., R. S. Jaiman, R. P. Bambharolia, and Vijay A. Patil. "Seed Biopriming– A Review." International Journal of Economic Plants 7, no. 1 (2020): 038–43. http://dx.doi.org/10.23910/2/2020.0359.
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There are various seed priming methods such as hydro priming, osmo priming, solid matrix priming, hormo-priming, chemo-priming, nutri-priming and biopriming being useful for enhancing the seed germination, seedling vigour and to overcome abiotic stress. Moreover to these advantages, of all priming methods only biopriming method gives an extra advantage of biotic stress management and thus gain special attention. Solubilisation or mobilization of soil macro and micronutrients, siderophore production, induction of plant growth promoting activities, induction of useful biochemicals, phytoalexin and defense-related enzymes and induced systemic resistance are the mechanisms involved in seed biopriming. Range of fungal or bacterial bio agents viz., Azotobacter, Rhizobium, Arthrobacter, Agrobacterium, Azospirillum, Enterobacter, Streptomyces, Bacillus, Burkholderia, Klebsiella, PSB, Pseudomonas fluorescence, Trichoderma viride, Trichoderma harzianum and Vesicular Arbuscular Mycorhiza, whether they are biofertilizer or biopesticide, may be useful as biopriming agents. Seed biopriming is useful in almost all the crops over the globe and is an eco-friendly substitute to chemical fungicides.
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Aslam, Shaik, Shambhu Chouhan, Vadugu Harish, and Shivani Lalotra. "Seed Priming: A Key to Sustainability in Drought Stress." International Journal of Environment and Climate Change 13, no. 8 (2023): 1871–82. http://dx.doi.org/10.9734/ijecc/2023/v13i82142.
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Abiotic stresses spot lights the field crops at all growth phases and results in significant yield losses in important crops, endangering the safety of the world's food supply. Numerous physiological, biochemical, and molecular tactics have been examined by crop researchers to battle drought stress/water limiting stress, but in the current environment, these measures are meager. It is so claimed that plants can be primed by various organic and inorganic stimulants for exceptional toughness under stressful circumstances. In order to confer tolerance, novel seed priming techniques are promising field of research in stress biology and crop stress management. Seed priming is the process of carefully hydrating seeds with germination stimulants so that pre- germinative metabolic activity can continue while the radicle's emergence is halted. The terms "hydro-priming," "osmo-priming," "halo-priming," "solid matrix priming," "bio-priming," and "hormonal priming" refer to various priming techniques. It will speed up and synchronize germination, improve plant growth and stand establishment, raise stress tolerance, improve fertilizer and water use efficiency, and have superior weed suppression effects. This review paper covers the physiological and biochemical changes through several seed priming techniques, Nano-priming and its significance in sustainable agriculture.
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Miljaković, Dragana, Jelena Marinković, Gordana Tamindžić, et al. "Bio-Priming of Soybean with Bradyrhizobium japonicum and Bacillus megaterium: Strategy to Improve Seed Germination and the Initial Seedling Growth." Plants 11, no. 15 (2022): 1927. http://dx.doi.org/10.3390/plants11151927.
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Bio-priming is a new technique of seed treatment that improves seed germination, vigor, crop growth and yield. The objective of this study was to evaluate the effectiveness of Bradyrhizobium japonicum (commercial strains) and Bacillus megaterium (newly isolated strains) as a single inoculant and co-inoculant during seed bio-priming to improve seed germination and initial seedling growth of two soybean cultivars. The treated seeds were subjected to germination test (GT), cold test (CT) and accelerated aging test (AAT). B. megaterium significantly improved all parameters in GT and CT; final germination, shoot length, root length, root dry weight, and seedling vigor index in AAT, as compared to control. In addition, co-inoculation significantly increased all parameters except shoot dry weight in GT; all parameters in CT; germination energy, shoot length, root length, and seedling vigor index in AAT, in comparison to the control. Moreover, Br. japonicum significantly improved the germination energy, shoot length, shoot dry weight, root dry weight, and seedling vigor index in GT; all parameters in CT; shoot length, root length, and seedling vigor index in AAT, compared with non-primed seeds. Thus, B. megaterium strains could be used in soybean bio-priming as a potential single inoculant and co-inoculant, following proper field evaluation.
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Jatana, Bhupinder Singh, Sajjan Grover, Hari Ram, and Gurjinder Singh Baath. "Seed Priming: Molecular and Physiological Mechanisms Underlying Biotic and Abiotic Stress Tolerance." Agronomy 14, no. 12 (2024): 2901. https://doi.org/10.3390/agronomy14122901.
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Seed priming is a state-of-the-art, low-cost, and environment-friendly strategy to improve seed germination, seed vigor, abiotic and biotic stress tolerance, and the yield of field and horticultural crops. Seed priming involves imbibing the seeds in a priming solution under a desired set of environmental conditions for a period followed by drying before the radicle protrusion. Several seed priming approaches including hydropriming, osmopriming, bio-priming, hormonal priming, nutrient priming, nanoparticle priming, and electropriming can be effectively employed under different environmental conditions to improve crop growth and stress resilience. Seed priming is known to trigger enzymatic, hormonal, physiological, transcriptomic, metabolomic, and proteomic regulations in seed embryos during seed germination and plant growth, which leads to faster and synchronized seed germination and higher abiotic and biotic stress tolerance in crop plants. Furthermore, seed priming can induce cross-tolerance between abiotic and biotic stressors and induce stress memory for higher resilience of the next generation to environmental stresses. The present review paper discusses the applications of seed priming in biotic and abiotic stress tolerance and the underlying abiotic and biotic stress tolerance physiological, biochemical, and molecular mechanisms of seed priming. Furthermore, we discuss the current challenges/bottlenecks in the widespread application of seed priming in crop production.
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Balaji, D. S., S. Arunkumar, D. Venkatakrishnan, and G. Sathiya Narayanan. "Impact of Different Seed Priming Treatments on Seed Yield in Foxtail Millet." Environment and Ecology 42, no. 2B (2024): 762–69. http://dx.doi.org/10.60151/envec/mkbt1613.
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The experiments were carried out both in the laboratory as well as in the field condition. Field experiments were conducted to determine the productivity of foxtail millet due to different seed priming treatments. Halo priming with KH2PO4 @ 2 %, CaCl2 @ 2%, osmo priming with Mannitol @ 2 %, PEG @ -15 Bars, bio priming with Pseudomonas fluorescens (LF) @ 15, Prosophis leaf extract @ 10 % with seed to solution ratio of 1:1 for the soaking duration of 8 h along with unprimed, Thiram @ 2g/kg and hydro primed seeds were evaluated for its productivity during kharif and rabi seasons. The results revealed that the crop performance with regard to growth parameters, physiological parameters, yield and yield attributing parameters was outperformed in halo-priming with 2% KH2PO4 for 8 hrs primed seeds during kharif season than rabi.
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Ceritoğlu, Mustafa, Murat Erman, Fatih Çığ, et al. "Bio-priming Treatment with PGPB Strains in Cowpea Production Increases Grain Yield and Net Income." Research in Agricultural Sciences 55, no. 2 (2024): 79–88. http://dx.doi.org/10.17097/agricultureatauni.1418425.
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In the 21st century, the use of beneficial microorganisms as biological fertilizers has become a notable phenomenon, driven by the ongoing search for sustainable solutions due to environmental issues associated with synthetic fertilizer use. This study aimed to investigate the effect of bio-priming with plant growth-promoting bacteria (PGPB) strains comparing them with synthetic fertilizer and rhizobium inoculation in Siirt ecological conditions. The field experiment was laid out according to a completely randomized design with four replications in the arable land of Siirt University (Siirt, Türkiye) during the 2019 summer season. Three synthetic fertilizer doses as diammonium phosphate (SF1: 100 kg ha-1, SF2: 200 kg ha-1, SF3: 300 kg ha-1) and seven biological fertilizer treatments (B1: TV61C, B2: TV62C, B3: TV126C, B4: TV24C, B5: TV53D, BMIX: TV119E+TV126C, RZB: Bradyrhizobium sp.) were compared with control (no fertilization+hydro-priming) in the study. The research results indicated that 300 kg ha-1 DAP and PGPB consortia showed the best results on agronomic characteristics. However, particularly when applied in the form of a consortium, PGPB strains exhibited performance very close to synthetic fertilization. Moreover, it was determined that 300 kg ha-1 DAP and PGPB consortia increased grain yield over hydro-primed plants by 54.6% and 42.4%, while they provided a net income of $654 and $721.6, respectively. Thus, bio-priming with PGPB increased higher net income compared with synthetic fertilizer due to lower treatment costs. In conclusion, bio-priming with PGPB strains has the potential of useful, sustainable and cost-effective strategy in cowpea production.
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Saudi, A. H., and Adawiya Sajid Mustafa Al-Rawi. "Effect of Seed Priming Duration with Bio-Stimulator (Appetizer) on Germination Characteristics and Seedling Emergence of Sorghum." IOP Conference Series: Earth and Environmental Science 1262, no. 5 (2023): 052034. http://dx.doi.org/10.1088/1755-1315/1262/5/052034.
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Abstract Two factorial experiments were conducted. One of them was laboratory experiment which was carried out at the Laboratory of Agricultural Engineering Sciences College, University of Baghdad during 2023. The other was conducted at the lath house with used the pots during spring season of 2023. The aim was to investigate the effect of seed priming duration with bio-stimulator (Appetizer) on germination characteristics and seedling emergence of Sorghum. The design of Lab. experiment was (CRD) while for the other experiment was (RCBD) with four replications. Each experiment consisted of two factors. The first factor included seeds priming treatments with bio-stimulator (Appetizer) concentrations (1, 2 and 3) ml l-1 and seeds soaking in distilled water, in addition to unsoaked seeds treatment (dry seed). The second factor was included seeds priming periods that is (8,16 and 24) h in room temperature. The results of Laboratory experiment showed that sorghum seeds primed with appetizer concentrations 3 ml l-1 gave highest averages of germination percentage in the first count, germination percentage in the final count, radical length, plumule length, seedling dry weight and seedling vigour index. While the comparision treatment (dry seed) gave the lowest averages for these characteristics and the priming period (24h) gave highest averages of germination percentage in the first count, germination percentage in the final count, radical length, plumule length, seedling dry weight and seedling vigour index. The results showed that iteration between the factors (seeds priming treatments with Appetizer and seed priming duration ) has significant effect in most characterstics. The results of pots experiment showed that sorghum seeds primed with appetizer concentrations 3 ml l-1 gave highest averages of emergence percentage in the first count and emergence percentage in the final count. The priming period (24h) gave highest averages of emergence percentage in the first count and emergence percentage in the final count.
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Ahmed, MGU, F. Khatun, and Z. Islam. "Effects of Osmotic, Thermal and Plant Growth Regulators Seed Priming on Different Wheat Varieties." Journal of Bio-Science 29, no. 2 (2021): 111–22. http://dx.doi.org/10.3329/jbs.v29i2.54960.
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Proper germination and proper growth of seedlings in conventional farming methods are often not achieved at the desired rate. The purpose of priming is to reduce germination time and improve stand and percentage of germination and increases vigour for fast and strong plant development. In order to evaluate the effect of seed priming on growth and yield of wheat the present study was conducted at Agronomy Farm House, Rajshahi University. Three wheat varieties (BARI Gom-24, BARI Gom-25 and BARI Gom-26) were tested with three groups of priming media (osmo-priming, thermos-priming and plant growth regulator priming) and control (non-soaked). Seeds were primed for 24 h at 25°C with four priming media, osmo-priming (1% NaCl, 2% KNO3, 2% KH2PO4, and 10% PEG), PEG-Priming (50 μM GA3 and 50 μM BAP) and thermo-priming (0°C, 5°C and 10°C) and control was without priming agent. All types of priming media were significantly different among their types for plant height (cm), tiller number and dry biomass (g). PEG was significantly different in osmo-priming, GA3 was significantly different in PGR-priming and 0°C was significantly different in thermo-priming. In case of vigor index, 2% KH2PO4 was suitable among osmo-priming, GA3 was better than BAP priming and 0°C was optimum among all temperature priming. Seed priming helps seeds to grow rapidly and evenly in the field, producing healthier and faster maturing crops with better yields. J. Bio-Sci. 29(2): 111-122, 2021 (December)
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Danie, Indra Yama, Rizal Muhammad, and Ali Muhammad. "Improvement of Sugarcane Seeds Drought Stress Tolerance by Invigoration using of Trichoderma as Bio-Primer." International Journal of Environmental & Agriculture Research 9, no. 4 (2023): 82–87. https://doi.org/10.5281/zenodo.7895476.
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<strong>Abstract</strong><strong>—</strong> Trichoderma used in this study resulted in a density of 1.4 x 10<sup>-9 </sup>with their morphology had septate mycelium, rounded or oval conidia shape and attached to each other, has a bright green color and branched conidiophores. The biopriming treatment had a significant effect on germination, germination rate, vigor index and total chlorophyll content of sugarcane that was stressed by drought. Soaking for a long time (24 hours) caused a decrease in the percentage of germination in sugarcane seeds by 12.69%, the vigor index of 1.06 and short immersion also caused a decrease in the percentage of germination by 10.61% and a decrease in the average vigor index by 21.74. The 12-hour soaking treatment was a better treatment than control to increasing germination rates under drought stress, it was 12.5% specifically and fast germination rate of 3.76 days. Sugarcane germination rates was reduced if the soaking process is carried out in brief time and vice versa. The highest vigor index value was produced by biopriming treatment for 12 hours, which was 81.74. Biopriming treatment using secondary metabolites of Trichoderma was increasing the total chlorophyll content in sugarcane germination by 0.22 g/ml compared to without soaking treatment.
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Jaiman, Rakesh Kumar, Sanjay Kumar Acharya, Naziya P. Pathan, et al. "In vitro effect of seed bio-priming techniques on seed germination and seedling vigour of few vegetable crops." Journal of Applied and Natural Science 12, no. 4 (2020): 702–9. http://dx.doi.org/10.31018/jans.v12i4.2422.
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Bio-priming can also be viewed as a new technique of seed treatment using biological agents to stimulate germination of seed and growth of the plant and further protecting the seed from soil-and seed-borne pathogens. The present investigation was carried out in vitro conditions on seed germination and seedling vigour of few vegetable crops viz. tomato (Solanum lycopersicum L.), brinjal (Solanum melongena L.), onion (Allium cepa L.) and chilli (Capsicum annuum L.), during 2015 and 2016. The treatments comprised viz. T1: Non primed seeds (Control), T2: Seed treatment with Carbendazim 2.5g/kg seed, T3: Hydro-priming for 6 hrs, T4: Hydro-priming for 12 hrs, T5: Hydro-priming for 18 hrs, T6: Biopriming with Trichoderma viride for 6 hrs, T7: Biopriming with T.viride for 12 hrs, T8: Biopriming with T.viride for 18 hrs, T9: Biopriming with Trichoderma harzianum for 6 hrs, T10: Biopriming with T. harzianum for 12 hrs, T11: Biopriming with T. harzianum for 18 hrs, T12: Biopriming with Pseudomonas fluorescens for 6 hrs, T13: Biopriming with P. fluorescens for 12 hrs and T14: Biopriming with P. fluorescens for 18 hrs. The results revealed that maxiumum germination percentage (92.92, 90.77,83.00 and 86.33), seedling length (32.38 cm, 29.35 cm, 31.75 and 31.60 cm), seedling fresh weight (2.07 g, 4.01 g, 3.05 g and 2.04 g), seedling dry weight (0.42 g, 0.86 g, 0.62 g and 0.42 g) and seedling vigour index (3008.11, 2664.00, 2635.00 and 2728.00) were recorded in T10 (bio priming with T. harzianum for 12 hrs) in tomato (S. lycopersicum L.), brinjal (S. melongena L.), onion (A. cepa L.) and chilli (C. annuum L.), respectively. Thus, it indicated that priming of seeds of these crops with T. harzianum/P. fluorescens/ T. viride for 12 hrs was very effective with respect to their vegetative growth along with the quality yield.
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Iqbal, Aliza, Usman Arshad, Sabahat Ayub, Ayesha Jabeen, and Muhammad Saeed. "Bio-Priming of Seed with Trichoderma sp. and Bacillus sp. Leads to Improved Morpho-Physiological Parameters Related to Drought Tolerance in Maize." Integrative Plant Biotechnology 1, no. 1 (2024): 43–51. http://dx.doi.org/10.55627/pbiotech.001.01.0669.
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Maize (Zea mays L.) holds an important position as a major grain crop in Pakistan flourishing across a diverse range of environmental conditions. The maize crop encounters various severe abiotic challenges with drought being the most concerning factor leading to diminished plant growth and yield. Seed priming a well-established and widely adopted technique serves as an effective mechanism to alleviate drought stress ultimately boosting both growth and grain yield. The primary objective of this study was to evaluate the impact of diverse seed priming methods on the growth and development of maize plants. Additionally, the study sought to assess the potential of various seed priming techniques in mitigating drought stress in maize. A pot experiment was conducted using a completely randomized design under natural conditions. The aim was to scrutinize the influence of different priming agents on morpho-physiological traits associated with water deficit conditions in maize. The gathered data underwent ANOVA analysis revealing variations in all parameters for both maize varieties at a 5% probability level. Specifically, the Pak-Afgoi variety displayed notable responsiveness to the seed priming approach in alleviating drought stress in maize. Furthermore, the findings underscored the evident and substantial impact of seed priming on both maize varieties across a range of studied characteristics under drought stress conditions. In conclusion, the present study suggests that seed priming holds the potential to enhance drought tolerance in maize.
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Şahin, Nihan, Levent Arın, Mehmet Uludağ, Zafer Şakacı, and Sırrı Kar. "Priming Applications with Mosquito (Diptera: Culicidae) Larva Rearing Water in White Cabbage Seeds (Brassica oleracea L. var. capitata F. alba cv. Yalova 1)." International Journal of Environment, Agriculture and Biotechnology 7, no. 4 (2022): 147–53. http://dx.doi.org/10.22161/ijeab.74.16.
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The starting material with good quality is a portent of a satisfactory and good harvest in vegetables grown from seedlings such as cabbage, a good beginning makes a good ending. Seed pre-applications promote uniform and rapid germination/emergence, leading to strong seedling formation. In this study, bio-based larval rearing water was used as an organic priming agent and its effect was compared with the effects of hydro priming. As a result of the study, the effects of both priming applications on germination and emergence were superior to the untreated control in terms of mean time and vigor, but there were no differences according to germination and emergence percentages. According to mean time of germination organic primed seeds germinated in the shortest time with 2.45 days compared to hydro primed seeds (2.77 days) and control (3.48 days). Likewise, this, organic primed seeds were emergence in 6.80 days, hydro primed seeds were emergence in 7.19 days and control seeds were emergence in 7.39 days. Vigor index of germination was 19.09 in organic priming, but it was 17.48 in hydro priming and 14.56 in control. On the other hand, vigor index of emergence was 6.39 in organic priming, 5.67 in control and, 5.28 in hydro priming applications. In addition, organic priming came to the fore among organic priming and hydro priming. It is thought that this positive effect may be due to the nitrogen and organic carbon content of larval rearing water.
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Bindu, B. "Pre-sowing seed bio-priming in okra (Abelmoschus Esculentus L.)." Journal of Krishi Vigyan 9, no. 1 (2020): 282–86. http://dx.doi.org/10.5958/2349-4433.2020.00175.0.
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Inuwa, A .B, Abubakar, A. S, Ibrahim, M. A, and Sulaiman, H. U. "Bio - priming and Antagonistic Potentials of Senna obtusifolia Endophytic Bacteria." UMYU Journal of Microbiology Research (UJMR) 2, no. 2 (2017): 77–81. http://dx.doi.org/10.47430/ujmr.1722.011.
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Endophytic microorganisms have continued to gain prominence as rich sources of useful compounds such as plant growth promoting chemicals, bioactive compounds among others. The present study aimed at evaluating the tomato seeds bio-priming and, antagonistic potentials of endophytic bacteria isolated from Senna obtusifolia. Endophytic bacteria harboured in the roots and leaves of S. obstusifolia were isolated using a combination of cultural, biochemical and microscopic techniques. The isolates were evaluated for possible applications as growth- promoting agents of tomato seeds and also, as antagonistic agents to the notorious plant pathogenic fungus Fusarium oxysporum. Diverse genera of bacteria were isolated from the plant and these, prominently include, Bacillus spp; Staphylococcus aureus; Escherichia coli; Enterobacter spp; Rhizobium spp and Pseudomonas spp. Although, tomato seeds bio-primed with Enterobacter spp germinated before all others, the germination period (4 days) was statistically the same (P< 0.05) as that yielded by the control (4.5). Similarly, tomato seeds treated with S. aureus yielded the highest number of leaves (2.5) and, this was also statistically the same as that yielded by the control (P < 0.05). All the isolates used in the evaluation of antagonistic activity yielded significantly larger (P > 0.05) zone of inhibition than the control (11.0 mm). Among these, Bacillus spp yielded the largest zone (21.6 mm). The study revealed that S. obstusifolia harbours endophytic bacteria that could inhibit the growth of the plant pathogen, F. oxysporum.
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., PRADEEP, DODDAGOUDAR S. R, UMESH HIREMATH, ARUNKUMAR HOSAMANI, and GNYANADEV x. B. "Effect of seed bio priming with endophytes on seedquality of soybean under induced drought stress." Journal of Farm Sciences 36, no. 02 (2023): 225–29. http://dx.doi.org/10.61475/jfm.v36i02.21.
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A lab experiment was conducted during 2021-22 atRaichur Karnataka to study the effect of seed bio priming withendophytes was examined on seed quality parameters of soybean(JS-335). The experiment was laid out in two factorial completelyrandomized design with four replications.Results showed that seedbio priming with the endophyte Trichoderma hamatum (1 x 103 conidiaml-1) recorded significantly higher seed germination (81.2%), shootlength (15.9 cm), root length (9.5 cm), seedling vigour index-I (2073),growth rate index (24.08), peak value (893), mean daily germination(10.15) under drought stress as compared to control.
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ElAzeiz, Eman H. Abd, Enas E. Yousif, and Walaa S. Elbatrawy. "Enhancement the Quality and Productivity of Canola (Brassica napus L.) Using Some Bio Stimulant Applications under Saline Soil Conditions." Journal of Global Agriculture and Ecology 16, no. 1 (2024): 8–27. http://dx.doi.org/10.56557/jogae/2024/v16i18507.
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Salinity is one of the most serious and significant challenges facing the agricultural sector. It directly damages soil irrecoverably and negatively affects the quality and productivity of many crops. Priming is a process of seeds treatment with a rapid soaking with drying the seeds before setting, using for regulating the germination process by managing and regulating the temperature and seed moisture content. Priming involves advancing the seed to enable fast and uniform emergence. Once, field’s condition (temperature and moisture) is appropriate, germination occurs in a much shorter time. Several benefits to seed priming, and they include: faster speed of emergence, enables seed to germinate and emerge even under adverse agro-climatic conditions, improves uniformity to optimize harvesting efficiency, increases vigor for fast and strong plant development and increases yield potential. Hence, how to use priming is still a challenge that remains to be solved. This experiment was carried out in two winter seasons 2021/2022 and 2022/2023 at Tag El-Ezz Research Station ARC in Egypt, using canola (Brassica napus L.) var. Serw 4 with adding two potassium humate treatments (zero as control and 2 kg.fed-1) as main plots, two selenium applying methods (priming and foliar) as sub main plot and four rates of selenium application (zero as control, 1.5, 3.0 and 4.5 ppm) as sub sub-plots and their interactions on canola plant growth, yield and yield components. Some chemical composition and oil percentages in canola seeds were analyzed. After harvest, soil's available N, P and K were determined. Results could be summarized as follow: Humate applied at 2 kg. fed-1 increased chlorophyl (a+b) by 3.84%, yield by 8.65% and oil content by 1.76% comparing with the control. Humate application alleviates adverse effect of salinity stress and maximize oil yield comparing with without humate application. Priming is more effective than foliar application. Selenium application at 1.5 ppm is the superior rate followed by 3.0 ppm, control and 4.5 ppm at the least. The interactions between humate and Canola seed priming in 1.5 ppm selenium mitigate the harmful effect of soil salinity on Canola and increased yield, productivity and quality.
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Munakata, Wataru, Qiang Liu, Tadashi Shimoyama, Manabu Sawaya, Takashi Umeda, and Kazuo Sugawara. "Ecabet sodium attenuates reactive oxygen species produced by neutrophils after priming with bacterial lipopolysaccharides." Luminescence 18, no. 6 (2003): 330–33. http://dx.doi.org/10.1002/bio.745.
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A.S. AHMED, P.S. PATRA, and R. SAHA. "Agronomic bio-fortification and productivity of maize (Zea mays L.) through zinc application." Indian Journal of Agronomy 66, no. 1 (2001): 97–102. http://dx.doi.org/10.59797/ija.v66i1.2828.
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A field experiment was conducted during the winter (rabi) season of 2018 and 2019 at instructional farm of Uttar Banga Krishi Viswavidyalaya, West Bengal, India, to find out a cost-effective method of zinc application for improving productivity and profitability of maize cv. Kaveri 2018 cultivation in randomized block design with 8 treatments and 3 replications. Soil application of 10 kg Zn/ha resulted in significantly tallest plant, maximum leaf-area index (LAI) and dry-matter accumulation. Yield attributes, viz. cob length, cob width, grains/row and grains/cob, were found highest with the application of 10 kg Zn/ha and showed 23.98 and 19.80% yield advantage over the control, which was followed by soil application of 5 kg Zn/ha, seed priming with 2% Zn solution and seed priming with 1% Zn solution + foliar application of 0.5% Zn at tasseling stage.
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Kumar, S. Arun, G. Sathiya Narayanan, and S. Ranjithrajaram. "Studies on Effect of Various Seed Priming Treatments on Seed Yield in Barnyard Millet (Echinochloa frumentacea) cv CO1." Environment and Ecology 41, no. 3 (2023): 1362–68. http://dx.doi.org/10.60151/envec/bxuz5450.
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Field investigation was carried out to study the effect of various seed priming treatments on seed yield in barnyard millet cv CO 1. The seeds of barnyard millet cv CO 1 were given with following seed treatments i.e., dry dressing with Thiram @ 2 g/kg, Hydropriming, bio priming with Pungam leaf extract @ 5%, Moringa leaf extract @ 5%, organo priming with Vermiwash @ 5%, Panchagavya @ 5%, halo priming with MnSO4 @ 2%, ZnSO4 @ 2% along with control. The primed seeds were evaluated for the growth parameters, physiological parameters, gas exchange parameters and yield attributing characteristics. The Moringa leaf extract @ 5% seed priming treatment recorded higher values for the growth parameters such as field emergence, plant height at 45 days and plant height at 90 days. It also recorded early days to first flowering, early days to 50 % flowering when compared to other treatments. The Moringa leaf extract @ 5% seed priming treatment registered significantly higher values for the physiological parameters such as chlorophyll content at 45 DAS, chlorophyll content at 90 DAS, net assimilation rate at 30-50 DAS and 50-95 DAS, leaf area and gas exchange parameters such as photosynthetic rate, transpiration rate and stomatal conductance. In addition to the above characters, Moringa leaf extract @ 5% seed priming treatment also registered significantly higher values for the various seed yield attributing characteristics such as panicle weight plant-1, panicle to seed recovery, seed yield plant-1, seed yield plot-1 and 1000 seed weight.
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Singh, Vivek, R. S. Upadhyay, B. K. Sarma, and H. B. Singh. "Seed bio-priming withTrichoderma asperellumeffectively modulate plant growth promotion in pea." International Journal of Agriculture, Environment and Biotechnology 9, no. 3 (2016): 361. http://dx.doi.org/10.5958/2230-732x.2016.00047.4.
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V., Sukanya. "An Overview: Mechanism Involved in Bio-Priming Mediated Plant Growth Promotion." International Journal of Pure & Applied Bioscience 6, no. 5 (2018): 771–83. http://dx.doi.org/10.18782/2320-7051.6508.
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Bavana Keerthi, A., P. Kishore Varma, S. L. Bhattiprolu, N. Kamakshi, and A. Janaki prasad. "Efficacy of bio-priming in enhancing blackgram seed germination and vigour." Andhra Agricultural Journal 71, no. 3 (2024): 323–27. https://doi.org/10.61657/aaj.2024.138.
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Jyothi, Chikkala, Sam A. Masih, and Ann Maxton. "Efficiency of Seed Bio-Priming Technique for Drought Management in Mungbean." Environment and Ecology 41, no. 4C (2023): 2798–804. http://dx.doi.org/10.60151/envec/fkkr6440.
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Drought stress is considered a severe treat to crop production as it adversely affects the morpho-physiological, biochemical and molecular functions of plants especially in short duration crops like mungbean. Water stress during the growth phase (called terminal drought) has become a threat to mungbean productivity. To reduce stress, “biopriming” has emerged as a new agricultural and sustainable technology. Biopriming is a coating process or treatment with PGPR, which is good at hydration control and improves pre-germination preparation without the appearance of radicles. Biopriming significantly increased Mungbam yield and composition and modulated the activity of antioxidants (superoxide dismutase, catalase, peroxidase, ascorbic acid and all phenolics) by 8 - 12% under normal and drought conditions. Seed application will increase the germination capacity of mung bean production and the plant will eventually tolerate drought stress.