Academic literature on the topic 'Bioactive triterpene'
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Journal articles on the topic "Bioactive triterpene"
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Mitova, Maya, Rilka Taskova, Simeon Popov, Ralf Günter Berger, Ulrich Krings, and Nedjalka Handjieva. "GC/MS Analysis of Some Bioactive Constituents from Carthamus lanatus L." Zeitschrift für Naturforschung C 58, no. 9-10 (2003): 697–703. http://dx.doi.org/10.1515/znc-2003-9-1018.
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AbstractSterols, triterpenes, volatiles, polar and other constituents in aerial parts of Carthamus lanatus were analyzed by gas chromatography-mass spectrometry. Over 90 compounds were identified most of them new for the species. Sitosterol and stigmasterol were the most abundant of 10 sterols identified in the sterol fraction. Taraxasterol, α- and β-amyrine prevailed in the triterpene fraction. Volatiles, sterols and a fraction of the dichloromethane extract showed strong cytotoxicity (Artemia salina assay).
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Barre, Juanita T., Bruce F. Bowden, John C. Coll, et al. "A bioactive triterpene from Lantana camara." Phytochemistry 45, no. 2 (1997): 321–24. http://dx.doi.org/10.1016/s0031-9422(96)00805-9.
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Kao, Chi, Amalini C. Jesuthasan, Karen S. Bishop, Marcus P. Glucina, and Lynnette R. Ferguson. "Anti-cancer activities of Ganoderma lucidum: active ingredients and pathways." Functional Foods in Health and Disease 3, no. 2 (2013): 48. http://dx.doi.org/10.31989/ffhd.v3i2.65.
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Ganoderma lucidum, commonly referred to as Lingzhi, has been used in Asia for health promotion for centuries. The anti-cancer effects of G. lucidum have been demonstrated in both in vitro and in vivo studies. In addition, the observed anti-cancer activities of Ganoderma have prompted its usage by cancer patients alongside chemotherapy. The main two bioactive components of G. lucidum can be broadly grouped into triterpenes and polysaccharides. Despite triterpenes and polysaccharides being widely known as the major active ingredients, the different biological pathways by which they exert their anti-cancer effect remain poorly defined. Therefore, understanding the mechanisms of action may lead to more widespread use of Ganoderma as an anti-cancer agent. The aim of this paper is to summarise the various bioactive mechanisms that have been proposed for the anti-cancer properties of triterpenes and polysaccharides extracted from G. lucidum. A literature search of published papers on NCBI with keywords “Ganoderma” and “cancer” was performed. Among those, studies which specifically examined the anti-cancer activities of Ganoderma triterpenes and polysaccharides were selected to be included in this paper. We have found five potential mechanisms which are associated with the anti-cancer activities of Ganoderma triterpenes and three potential mechanisms for Ganoderma polysaccharides. In addition, G. lucidum has been used in combination with known anti-cancer agents to improve the anti-cancer efficacies. This suggests Ganoderma’s bioactive pathways may compliment that of anti-cancer agents. In this paper we present several potential anti-cancer mechanisms of Ganoderma triterpenes and polysaccharides which can be used for the development of Ganoderma as an anti-cancer agent.Keywords: Ganoderma lucidum, cancer, bioactive pathways, triterpene, polysaccharide
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Villanueva-Bermejo, David, Erika Vázquez, Marisol Villalva, et al. "Simultaneous Supercritical Fluid Extraction of Heather (Calluna vulgaris L.) and Marigold (Calendula officinalis L.) and Anti-Inflammatory Activity of the Extracts." Applied Sciences 9, no. 11 (2019): 2245. http://dx.doi.org/10.3390/app9112245.
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Heather (Calluna vulgaris L.) and marigold (Calendula officinalis L.) are two rich sources of bioactive pentacyclic triterpenes. The supercritical carbon dioxide (SCCO2) extraction of these two plants was explored at a pressure range of 25–50 MPa, 50 °C, with or without fractionation, different extraction times (1.5–4.0 h) and using ethanol as a co-solvent (0 and 10% w/w). In order to determine potential synergisms, a combined extraction (heather + marigold 50:50) was also studied. In general, higher extraction yields were achieved when the co-solvent was added. Higher concentrations of total triterpenic acids were obtained in heather extracts, specially using ethanol. The co-solvent did not increase the terpene concentration in marigold extracts. For the combined extraction, an antagonist effect in the triterpene concentration was observed in absence of a co-solvent, whereas a synergistic effect was exhibited in its presence, especially for ursolic acid. In general, the extracts showed a certain anti-inflammatory effect, although a straight correlation with the analyzed triterpenic acids concentration was not exhibited. Moreover, the combined extraction exposed a similar anti-inflammatory activity in comparison with the individual plant extracts.
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Novakovic, Miroslav, Jasmina Nikodinovic-Runic, Jovana Veselinovic, et al. "Bioactive Pentacyclic Triterpene Ester Derivatives fromAlnus viridisssp.viridisBark." Journal of Natural Products 80, no. 5 (2017): 1255–63. http://dx.doi.org/10.1021/acs.jnatprod.6b00805.
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Jamila, Nargis, Naeem Khan, Imran Khan, Amir Atlas Khan, and Sadiq Noor Khan. "A bioactive cycloartane triterpene from Garcinia hombroniana." Natural Product Research 30, no. 12 (2015): 1388–97. http://dx.doi.org/10.1080/14786419.2015.1060594.
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Geană, Elisabeta-Irina, Corina Teodora Ciucure, Roxana Elena Ionete, et al. "Profiling of Phenolic Compounds and Triterpene Acids of Twelve Apple (Malus domestica Borkh.) Cultivars." Foods 10, no. 2 (2021): 267. http://dx.doi.org/10.3390/foods10020267.
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Apple (Malus domestica Borkh.), a popular and widely cultivated fruit world-wide, contains bioactive compounds responsible for their health benefits. Here we report the amounts of some bioactive compounds: two major triterpenes (oleanolic and ursolic acids) and polyphenols (phenolic acids, flavan-3-ols, flavonoids and t-resveratrol), together with bioactive properties of twelve apple cultivars measured by chromatographic and spectrophotometric methods. Significant variations were found comparing the bioactive potential of the investigated cultivars. High contents of phenolic acids were identified in the Montuan, Golden Delicious and Cretesc cultivars, while the most flavonoid dominant was the Richard cultivar. Starkrimson, Jonatan, Beliy Naliv and Richard cultivars present higher antioxidant capacity. Oleanolic acid ranged from 11 to 83 mg/g apple extract, while ursolic acid ranged from 55 to 436 mg/g apple extract, with higher amounts in Richard and Montuan cultivars. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) allowed the discrimination of apple cultivars depending on polyphenolic and triterpene acids composition. Caffeic acid, gallic acid and epicatechin were identified as the main bioactive compounds in Starkrimson, Jonathan, Beliy Naliv and Richard cultivars, while ursolic and oleanolic acids were identified in high amounts in Richard, Montuan, Golden Delicious, Idared and Beliy Naliv apple cultivars. The results obtained in this study will contribute to the understanding of the bioactive composition of apples as well as the importance of their capitalization to obtain value-added products that promote human health.
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Ludeña-Huaman, Michael A., and Deborah A. Ramos-Inquiltupa. "Determination of the content of ursolic and oleanolic acid in the cuticular wax of fruits of different species of Rosaceae." Revista Colombiana de Química 48, no. 2 (2019): 15–20. http://dx.doi.org/10.15446/rev.colomb.quim.v48n2.77046.
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Ursolic acid (UA) and oleanolic acid (OA) are two widely distributed triterpenes in fruits, especially those belonging to Rosaceae family. These triterpene isomers are of great pharmacological interest due to their multiple bioactive properties. For this reason, the objective of this study was to determine the content of UA and OA extracted from the cuticular wax of five highly edible fruits (quince, loquat, pear, peach and apple) all belonging to the Rosaceae family. The acids were analyzed by high performance liquid chromatography. Both UA and OA are present in all these fruits, however, UA is in greater quantities.
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Silva, Maria, Juceni P. David, Lidércia C. R. C. Silva, et al. "Bioactive Oleanane, Lupane and Ursane Triterpene Acid Derivatives." Molecules 17, no. 10 (2012): 12197–205. http://dx.doi.org/10.3390/molecules171012197.
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González, A., N. Alvarenga, I. Bazzocchi, A. Ravelo, and L. Moujir. "A New Bioactive Norquinone-Methide Triterpene fromMaytenus scutioides." Planta Medica 64, no. 08 (1998): 769–71. http://dx.doi.org/10.1055/s-2006-957581.
Full textDissertations / Theses on the topic "Bioactive triterpene"
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Eaton, Alexander Lee. "Isolation and Synthesis of Bioactive Compounds from Plants." Diss., Virginia Tech, 2015. http://hdl.handle.net/10919/64367.
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As a part of a continuing search for bioactive compounds with the International Cooperative Biodiversity Group (ICBG), and in collaboration with the Natural Products Discovery Institute of the Institute for Hepatitis and Virus Research (IHVR), twelve plant extracts were investigated for their antiproliferative activity against the A2780 cell line, three plant extracts were investigated for their antimalarial activity against Plasmodium falciparum, and three plant extracts were investigated for their anti-inflammatory activity (PPAR-y inhibition). Bioassay-guided fractionation of extracts led to the identification of four new antiproliferative compounds (2.1-2.3, 3.1), five new anti-inflammatory compounds (6.4a, 6.5a-b, 6.6a, 6.6c), and twenty-eight known compounds from eight of the extracts. In addition, mallotojaponin C, an antimalarial natural product, and derivatives were synthesized and investigated for their antimalarial activity.<br>Ph. D.
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Haas, Christiane, Karl-Christoph Hengelhaupt, Sibylle Kümmritz, Thomas Bley, Atanas Pavlov, and Juliane Steingroewer. "Salvia suspension cultures as production systems for oleanolic and ursolic acid." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-216350.
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Oleanolic and ursolic acid (OA and UA) are triterpenic acids with diverse biological activities that are of interest to the pharmaceutical industry. To investigate the scope for producing these compound using cell suspension cultures of Salvia species, calli from S. officinalis, S. virgata and S. fruticosa were induced using several plant growth regulator (PGR) combinations. Eleven lines were selected for suspension induction from a pool of calli. Six suspension cultures were established successfully and cultivated in the Respiration Activity MOnitoring System® (RAMOS®) to obtain online data on their growth kinetics and to establish appropriate sampling schedules for the determination of their OA and UA production. Based on their observed growth behaviour, OA and UA contents, and aggregation properties, one suspension culture from each studied Salvia species was selected for further optimisation. The μmax values for these suspension cultures ranged from 0.20 to 0.37°d-1, their OA and UA contents were greater than 1.3 and 1.2 mg g-1, respectively, and they afforded maximum volumetric yields of 21.0 mg l-1 for OA and 32.8 mg l-1 for UA. These results will be useful in the development of a refined Salvia suspension-based process for OA and UA production.
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Haas, Christiane, Karl-Christoph Hengelhaupt, Sibylle Kümmritz, Thomas Bley, Atanas Pavlov, and Juliane Steingroewer. "Salvia suspension cultures as production systems for oleanolic and ursolic acid." Springer, 2014. https://tud.qucosa.de/id/qucosa%3A30075.
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Oleanolic and ursolic acid (OA and UA) are triterpenic acids with diverse biological activities that are of interest to the pharmaceutical industry. To investigate the scope for producing these compound using cell suspension cultures of Salvia species, calli from S. officinalis, S. virgata and S. fruticosa were induced using several plant growth regulator (PGR) combinations. Eleven lines were selected for suspension induction from a pool of calli. Six suspension cultures were established successfully and cultivated in the Respiration Activity MOnitoring System® (RAMOS®) to obtain online data on their growth kinetics and to establish appropriate sampling schedules for the determination of their OA and UA production. Based on their observed growth behaviour, OA and UA contents, and aggregation properties, one suspension culture from each studied Salvia species was selected for further optimisation. The μmax values for these suspension cultures ranged from 0.20 to 0.37°d-1, their OA and UA contents were greater than 1.3 and 1.2 mg g-1, respectively, and they afforded maximum volumetric yields of 21.0 mg l-1 for OA and 32.8 mg l-1 for UA. These results will be useful in the development of a refined Salvia suspension-based process for OA and UA production.
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Carletti, Isabelle. "Étude de molécules bioactives isolées d'éponges marines de l'Océan Indien." Nice, 2000. http://www.theses.fr/2000NICE5469.
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Le but de cette étude a été de rechercher des molécules bioactives dans des organismes marins. Nous avons entrepris les études chimiques de trois éponges marines récoltées dans l'Océan Indien. Après séparation et purification par extraction liquide et chromatographie, nous avons isolé et identifié 14 molécules, dont 3 structures nouvelles. Les déterminations structurales ont été réalisées à l'aide des méthodes spectrales usuelles, la résonance magnétique nucléaire et la spectrométrie de masse en particulier. Les études chimiques de Liosina granularis et Iotrochota purpurea ont été réalisées à l'aide du criblage biologique utilisant le test des oeufs d'oursin, et nous ont mené à l'isolement de bases puriques, de nucléosides, de dicétopipérazines et de 6-bromo-indoles, dont un dérivé 6-bromo-3-indolinone original que nous avons nommé matémone. Nous avons également recherché des molécules bioactives dans Axinella cf. Bidderi en ciblant, par un criblage chimique en spectrométrie de masse, une famille de triterpènes aux propriétés cytotoxiques. Nous avons ainsi isolé et identifié : la sodwanone A, la yardénone et deux dérivés nouveaux, la 12-hydroxy-yardénone et la 3-dihydro-yardénone.
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Habib, Lamice. "Étude des propriétés membranaires des vésicules lipidiques incorporant des triterpènes oxygénés bioactifs d'origine végétale : application à la cucurbitacine E et à l'érythrodiol." Thesis, Lyon 1, 2014. http://www.theses.fr/2014LYO10022/document.
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La cucurbitacine E et l'érythrodiol sont des triterpènes naturels oxygénés ayant respectivement un squelette tétra et pentacyclique. Ils sont reconnus pour leurs diverses propriétés biologiques. Dans ce travail de thèse, nous étudions leur interaction avec les membranes des vésicules lipidiques dans le but de mieux comprendre leur pharmacodynamie. Nous avons préparé des liposomes en absence et en présence de la cucurbitacine E et de l'érythrodiol par les techniques d'évaporation en phase inverse suivie d'une extrusion, d'hydratation du film lipidique et d'injection d'éthanol. Les caractéristiques physicochimiques des vésicules lipidiques incorporant ou non la molécule triterpénique ont été étudiées par des techniques adéquates. Les analyses de la cucurbitacine E et de l'érythrodiol par la chromatographie liquide à haute performance ont montré que leurs taux d'incorporation dans les liposomes sont élevés. Les mesures de taille obtenues par la diffusion dynamique de la lumière ont démontré que les liposomes incorporant les triterpènes présentent une taille moyenne inférieure à celle des liposomes témoins. Les images obtenues par la microscopie électronique à transmission ont confirmé la formation de vésicules sphériques. Les mesures des dimensions des vésicules observées par la microscopie à force atomique (AFM), ont révélé que les liposomes incorporant la cucurbitacine E sont plus hauts et résistent mieux à la force exercée par la pointe AFM que les liposomes témoins. Par ailleurs, les liposomes incorporant l'érythrodiol sont plus fragiles que les liposomes témoins et ont tendance à éclater en bicouches lipidiques à la surface du support. Les courbes thermiques obtenues par la calorimétrie différentielle à balayage ont permis de conclure que la cucurbitacine E est localisée à l'interface polaire-apolaire de la membrane liposomiale alors que l'érythrodiol s'insère entre les chaînes acyles des phospholipides et aboutit à la formation des domaines hétérogènes au niveau de la membrane. La cinétique de libération de la sulforhodamine B, mesurée par la spectroscopie de fluorescence, a révélé que la membrane liposomiale devient, en présence de la cucurbitacine E, plus perméable à la sulforhodamine B incorporée dans la phase aqueuse interne. L'ensemble des résultats suggère que la cucurbitacine E et l'érythrodiol interagissent avec la membrane lipidique et affectent ses propriétés physico-chimiques. Leur effet sur la membrane ne semble pas être similaire. Des études ultérieures impliquant d'autres triterpènes sont envisagées pour identifier le (s) motif (s) structural (aux) et les paramètres physico-chimiques régissant leur interaction et localisation membranaire<br>Cucurbitacin E and erythrodiol are natural oxygenated triterpenes having respectively, a tetra and pentacyclic skeleton. They are known for their numerous biological properties. In this thesis, we studied their interaction with the membranes of lipid vesicles to better understand their pharmacodynamics. We have prepared liposomes in the absence and presence of cucurbitacin E and erythrodiol using the reverse phase evaporation technique followed by extrusion, the hydration of lipid film and the ethanol injection techniques. The physicochemical characteristics of lipid vesicles incorporating or not the triterpenic molecules were investigated by appropriate techniques. The determination of cucurbitacin E and erythrodiol in the vesicles by high performance liquid chromatography showed high incorporation efficiencies of both triterpenes. Size measurements obtained by dynamic light scattering showed that liposomes incorporating triterpenes were smaller than empty liposomes. The images obtained by transmission electron microscopy confirmed the formation of spherical vesicles. Measurements of vesicles dimensions by atomic force microscopy (AFM) demonstrated that liposomes incorporating cucurbitacin E were higher and more resistant to the force exerted by the AFM tip than the blank liposomes. Liposomes incorporating erythrodiol were more fragile and tend to break up into lipid bilayers on the mica surface. Results obtained by differential scanning calorimetry suggested that cucurbitacin E is localized at the polar-apolar interface of the liposomal membrane while erythrodiol is inserted between the acyl chains of the phospholipids leading to the formation of heterogeneous lipid domains. The release kinetics of the sulforhodamin B encapsulated into the aqueous phase and measured by fluorescence spectroscopy revealed that the liposomal membrane becomes in the presence of cucurbitacin E, more permeable to this probe. The overall results suggest that cucurbitacin E and erythrodiol affect differently
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Tripoteau, Ludovic. "Valorisation des coproduits de l'holothurie Cucumaria frondosa par l'étude d'extraits bioactifs et approche écotoxicologique des métabolites secondaires relargués en situation de stress." Thesis, Lorient, 2015. http://www.theses.fr/2015LORIS368/document.
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La transformation des produits marins génère d’importantes quantités de rejets. La majorité est transformée pour des applications de masse avec une faible valeur ajoutée. Au Nouveau Brunswick, 85 000 tonnes de produits marins sont transformés annuellement et la moitié est peu ou pas valorisée, c’est le cas de l’holothurie Cucumaria frondosa. Face aux différentes pressions de leur environnement, et notamment la prédation, ces invertébrés marins ont évolué en développant des métabolites d’une large chimiodiversité, ce qui en fait d’excellents candidats pour le développement de substances naturelles bioactives. L’objectif de cette étude a tout d’abord été de chercher à valoriser les coproduits majeurs de Cucumaria frondosa en constituant une large librairie d’extraits et en évaluant, par fractionnement bioguidé, les activités antiherpétiques in vitro des fractions obtenues par différents procédés d’extraction (extractions par solvants et hydrolyses enzymatiques). Nous avons pu mettre en évidence la présence de fractions anti-VHS-1 (Virus Herpes Simplex de type 1). L’hydrolyse enzymatique s’est avérée être une technique efficace pour la génération de substances actives. Le bulbe aquapharyngé peu considéré d’un point de vue industriel représente un réel potentiel pour l’extraction de composés anti-VHS-1, et notamment lorsque celui-ci est hydrolysé par la papaïne en conditions contrôlées. L’activité antiherpétique la plus efficace a été reliée à la présence de molécules à haut poids moléculaires. Dans un second temps, nous avons cherché à comprendre et à mettre en évidence les mécanismes de défense de l’holothurie par l’étude de ces métabolites secondaires relargués en situation de stress. Les métabolites ont été évalués sur différents modèles écotoxicologiques et les molécules actives ont été caractérisées par fractionnement bioguidé par l’utilisation de modèle d’évaluation de cytotoxicité, en parallèle à différents procédés analytiques. L’étude a confirmé l’existence et la production de composés toxiques relargués en situation de stress. Ces métabolites secondaires ont montré une forte toxicité sur différents modèles écotoxicologiques chronique et aiguë. Le fractionnement bioguidé par couplage analytique nous a conduit à l’identification de plusieurs composés du type glycoside triterpénique et notamment le Frondoside A retrouvé dans la fraction responsable de la cytotoxicité. L’étude a permis d’optimiser l’extraction de ce composé d’un intérêt biologique prometteur sans pour autant entraîner la mort de l’animal<br>The transformation of marine products generates huge quantities of rejects. The majority of these rejects is used for an upgrading of products with low added value. In New Brunswick, 85 000 tons of marine products are transformed annually, the half is little or not upgraded, that is the case of the holothurian Cucumaria frondosa. Confronted with environmental pressures, and especially predation, the marine invertebrates have evolved and developed various metabolites of different chemical classes. Thus, these metabolites represent ideal candidates for the development of new natural bioactive substances. The objectives of this study was first based on the upgrading of the major byproducts from Cucumaria frondosa by creating a wide library of extracts obtained by different type of extractions (solvent extractions and enzymatic hydrolysis) and then, by bioguided fractionation, evaluating the in vitro anti-HSV-1 (Herpes Simplex Virus, type 1) activities of the fractions. We showed the presence of potent antiherpetic fractions without cytotoxicity on mammalian cells. The enzymatic hydrolysis has been demonstrated to be efficient for the generation of high molecular weight active substances regarding HSV-1. The aquapharyngeal bulb, considered to be less used industrially, represents a real potential for the extraction of anti-HSV-1 compounds, and especially when hydrolyzed by papain under controlled conditions. Secondly, we have searched to understand the mechanism of defense of the holothurian under stress conditions. The secondary metabolites involved were evaluated on different ecotoxicological models. This work has confirmed the existence and the production of toxic compounds released under stress conditions. These secondary metabolites have shown a strong toxicity on acute and chronic ecotoxicological models. The bioguided fractionation assisted by analytical evaluation led to the identification of several triterpene glycosides, and especially the Frondoside A into the cytotoxic fraction. This study has permitted the optimization of the upgrading of the byproducts from Cucumaria frondosa by the extraction of antiherpetic molecules without cytotoxicity and the identification and the characterization of the defense
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Grigoraş, Cristina-Gabriela. "Valorisation des fruits et des sous-produits de l'industrie de transformation des fruits par extraction des composés bioactifs." Phd thesis, Université d'Orléans, 2012. http://tel.archives-ouvertes.fr/tel-00772304.
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Les fruits et les sous-produits de l'industrie de transformation des fruits sont obtenus chaque année en quantités importantes partout dans le monde. Les cerises et les résidus de pommes sont parmi les sources végétales les plus riches en composés bioactifs qui sont reconnus pour leurs effets bénéfiques sur l'organisme humain. Des méthodes appropriées d'extraction (macération, extraction par solvant pressurisé, extraction assistée par ultrasons, extraction assistée par microondes), d'analyse (HPTLC, HPLC-UV-DEDL, HPLC-MS, SFC) et de fractionnement (CPC, HPLC semi-préparative, extraction liquide-liquide) ont été mises au point pour la récupération, l'identification et la purification des composés bioactifs de cerises (Prunus avium) et de résidus de différentes variétés de pommes (Royal Gala, Golden, Granny Smith, Pink Lady). Les résultats obtenus au cours des travaux de recherches indiquent le fait que ces sources végétales contiennent des composés phénoliques (cerises, résidus de pommes) et des composés triterpéniques (résidus de pommes) qui peuvent être valorisés par incorporation dans différentes formulations alimentaires, pharmaceutiques, cosmétiques etc.
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"Phytochemical characterization and supercritical fluid extraction of bioactive triterpenes from ganoderma lucidum." Thesis, 2006. http://library.cuhk.edu.hk/record=b6074197.
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Aims. The objectives of this study were (i) to isolate and characterize by conventional column chromatography, structurally diverse triterpenes from G. lucidum to serve as chemical markers; (ii) to develop a high performance liquid chromatography (HPLC) method for the quality control and/or standardization of Lingzhi-containing products; (iii) to utilize and optimize operating conditions for the newer extraction technology: supercritical fluid extraction (SFE), in order to maximize yields of bioactive triterpenes, and to reduce time and costs.<br>Background. The dried fruiting body of Ganoderma lucidum, commonly known as Lingzhi, has been used extensively as a traditional Chinese medicine (TCM) for many centuries not only in China, but also in other countries such as Japan and Korea. In recent years, Lingzhi has also become a popular health supplement in many Western countries. The chemical composition of Lingzhi is complex, but it has been well documented that the lipophilic triterpenoid class of compounds possess a range of biological effects that include antitumor, immunomodulatory, cardiovascular, respiratory and antihepatotoxic activity. A major drawback in TCM research has been the lack of authentic chemical standards, and efficient methods for the extraction and analysis of bioactive fractions and/or single components. Conventional extraction methods for G. lucidum are time-consuming and laborious, and often result in low yields of useful chemical constituents.<br>Conclusion. This study enabled the development of a method for the simultaneous analysis of structurally diverse triterpenes with remarkably different chromatographic profiles. The isolated triterpenes, as chemical markers, and the HPLC method can readily be used for quality control and/or standardization purposes in evaluating Lingzhi-containing products. Optimization of operating conditions for SFE facilitated the rapid and selective extraction of acidic triterpenes from raw G. lucidum in significantly higher yields.<br>Methods. Raw material of G. lucidum was extracted with 80% ethanol; subjected to repeated column chromatography to purify triterpenes; and characterized by NMR (1H and 13C) and mass spectroscopy. Isolated lipophilic triterpenes were qualitatively and quantitatively analyzed by reversed-phase HPLC using an ODS column (150 x 4.6 mm) and PDA detection at 256 nm. The assay was validated over appropriate concentration ranges and benzophenone was used as an internal standard. Supercritical fluid extraction of G. lucidum was carried out using a commercial supercritical fluid extractor system Thar, SFE-1000M. Briefly, the raw powder of G. lucidum was soaked in ethanol containing 10% aqueous ammonia for 30 minutes prior to extraction. Extractions were performed at temperatures of 40, 50 and 60°C; and pressures of 200, 250, 300 and 350 bar; 5% ethanol was used as the co-solvent; and the flow rate of CO 2 was set at 20 g/min.<br>Results. Eight compounds were isolated and identified from G. lucidum: four triterpenes; namely, lucidenic acid N, ganoderic acid B, ganodermanontriol, and ganodermadiol; two steroids; ergosterol-7, 22-dien-3beta-ol and ergosterol peroxide; and two fatty acids, oleic acid and tetracosanoic acid. The four triterpenes were utilized as chemical markers, and the developed HPLC method was able to simultaneously analyze the structurally diverse components with good resolution. The total analysis run time was 110 min, and retention times (tR) were 14.88, 18.96, 63.88 and 90.73 min respectively, and the eluting system was a mixture of three solvents, methanol (A), acetonitrile (B) and 2% acetic acid solution (C): 0-22 min, 5% A, 25% B and 70% C; 22-85 min, gradient elution, the ratio changed gradually to 5% A, 80% B and 15% C; 85-110 min, gradient elution, the ratio changed gradually to 5% A, 85% B and 10% C. The validated HPLC method and the isolated chemical markers were effectively applied to determine the triterpenoid contents in a variety of commercial Lingzhi products. Supercritical fluid extraction conditions of: pressure 300 bar and temperature 50°C, gave the highest yields of triterpene-containing extracts. HPLC analysis of the SFE extracts showed predominantly acidic triterpenes such as lucidenic acid N and ganoderic acid B.<br>Hong Xin.<br>"December 2006."<br>Advisers: Ho Yee Ping; Albert H. L. Chow.<br>Source: Dissertation Abstracts International, Volume: 68-09, Section: B, page: 5968.<br>Thesis (Ph.D.)--Chinese University of Hong Kong, 2006.<br>Includes bibliographical references (p. 149-175).<br>Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.<br>Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web.<br>Abstracts in English and Chinese.<br>School code: 1307.
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Ribeiro, Diogo Rafael Pacheco. "Bioactive compounds separation with simulated moving bed technology." Master's thesis, 2019. http://hdl.handle.net/10773/30277.
Full textAbstract:
The pharmaceutical industry is developing a growing interest in natural compounds with biological and nutritional properties. Betulinic and oleanolic acids are two naturally occurring triterpenic acids, which possess such characteristics. These two compounds can be extracted from natural resources, i.e., from the bark of Eucalyptus globulus. However, their separation is difficult because these compounds present similar structures (they are constitutional isomers).
In this work, the chromatographic separation of betulinic and oleanolic acids from representative extracts of E. globulus was studied. High performance liquid chromatography (HPLC) assays were initially performed using an Acclaim C30 column as stationary phase. Through these tests it was found that the mobile phase methanol/acetonitrile 50/50 (%, v/v) is the most suitable for the separation of both triterpenic acids.
In order to determine the equilibrium constants (𝐻𝑖) and the global linear driving force mass transfer coefficients (𝐾𝐿𝐷𝐹), several breakthrough experiments of pure acids (unary solutions) were carried out. The two parameters were correlated using experimental data and then validated by simulating the breakthrough curve of a binary mixture containing the same proportion of betulinic and oleanolic acids found in E. globulus extracts. The outcomes of all experiments were modeled using Carta’s analytical solution. In parallel, the method of moments was also used to obtain the transport parameters. The results were consistent.
Lastly, the separation of betulinic and oleanolic acids by simulating moving bed (SMB) has been studied, carrying out simulations using the transport parameters obtained before from the breakthrough assays. Two different column configurations (1-1-1-1 and 2-2-2-2) and column lengths (from 10 to 25 cm) were adopted. It was concluded that using an SMB unit is able to separate betulinic and oleanolic acids with purity higher than 99 % both in extract and raffinate for all the scenarios presented.
Posteriorly, the recovery of both triterpenic acids from the extract and raffinate streams of a future process was also investigated, taking advantage of their low solubility in water. Accordingly, the solubility of each triterpenic acid in solvent mixtures of increasing water concentration was experimentally measured and discussed.<br>A indústria farmacêutica tem manifestado um crescente interesse em compostos naturais com propriedades biológicas e nutricionais. Os ácidos betulínico e oleanólico são dois ácidos triterpénicos que ocorrem naturalmente na natureza e que possuem tais características. Estes dois compostos podem ser extraídos de recursos naturais, tais como da casca do Eucalyptus globulus. No entanto, a sua separação é difícil, pois os compostos apresentam estruturas muito semelhantes (são isómeros constitucionais).
Neste trabalho estudou-se a separação cromatográfica de uma amostra representativa dos extratos de E. globulus dos ácidos betulínico e oleanólico. Inicialmente foram realizados testes cromatográficos em cromatografia líquida de alta eficiência, recorrendo à fase estacionária Acclaim C30. Através destes ensaios determinou-se que a fase móvel metanol/acetonitrilo 50/50 (%, v/v) é a mais adequada para a separação destes ácidos triterpénicos.
De modo a averiguar as constantes de equilíbrio (𝐻𝑖) e os coeficientes globais de transferência de massa (𝐾𝐿𝐷𝐹), foram determinadas as curvas de rutura dos ácidos puros (soluções unárias). Estes parâmetros foram correlacionados recorrendo a dados experimentais e posteriormente validados pela simulação da curva de rutura de uma mistura binária contendo a mesma proporção de ácido betulínico e oleanólico encontrada no E. globulus. Os dados advindos deste ensaio foram modelados pela solução analítica de Carta. Paralelamente, foi também usado o método dos momentos para obter os parâmetros de transporte. Os resultados obtidos foram consistentes.
Por fim, estudou-se a separação dos ácidos betulínico e oleanólico por leito móvel simulado (SMB), realizando simulações utilizando os parâmetros de transporte obtidos anteriormente pela modelação das curvas de rutura. Foram adotadas duas configurações diferentes (1-1-1-1 e 2-2-2-2) e diferentes comprimentos de coluna (variando dos 10 aos 25 cm), usando os parâmetros de transporte obtidos. Foi concluído que usando uma unidade SMB é possível separar os ácidos betulínico e oleanólico com purezas superiores a 99 % tanto no extrato como no rafinado para todos os cenários apresentados.
Posteriormente, foi analisada a recuperação dos ácidos triterpénicos das correntes de extrato e rafinado de um futuro processo, tirando vantagem da sua baixa solubilidade em água. Por conseguinte, a solubilidade de cada ácido triterpénico em misturas de solventes com o aumento da concentração de água foi medida e discutida.<br>Mestrado em Engenharia Química
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Santos, Cristiana Areia dos. "Bioactive compounds separation with simulated moving bed technology." Master's thesis, 2018. http://hdl.handle.net/10773/25696.
Full textAbstract:
Natural products have been progressively forgotten by major pharmaceutical
research companies as sources for new drugs in the last half century.
Currently one observes a change of strategy, which is promoting a renewed
interest on the integration of natural products into pharmaceuticals. Triterpenic
acids are a promising group of plant secondary metabolites occurring
in cuticular waxes covering fruits, owers, and leaves, providing a wide variety
of bene cial biological activities. In this dissertation it was studied the
oleanolic and ursolic acids isolation by chromatographic tecnique of simulated
moving bed (SMB). The seperation of triterpenic acids is complex due
their similar structure. EgiChem laboratory (CICECO, UA) has a SMB unit,
but it is necessary to carry out preliminary experiments in order to select
the best mobile phase and optimal operating conditions for separation of
both acids. For that, it was analyzed the infuence of the solvent/mixture
of solvents on the chromatographic separation by measuring selectivity, retention
factor and resolution. It was tested a triacontyl column (Acclaim
C30), and the best results were obtained with a mobile phase of methanol/
water 95/5 (%, v/v). Then, breakthrough curves have been measured
in order to determine process parameters like the equilibrium isotherms (constants
Hi) of both oleanolic and ursolic acids, and the global linear driving
force (LDF) coe cients of mass transfer (KLDF,i). Data modeling was accomplished
using Klinkenberg equation, which is specifcially applicable to
linear systems. The equilibrium constants of oleanolic and ursolic acdis were
HOA= 2.06 and HUA= 2.16, respectively, and global LDF coe cients were
KLDF,OA= 30.48 min-1 and KLDF,UA= 101.45 min-1 with average deviaions
of AADROA=18.29 % and AADRUA=26.88 %. Finally the simulation of a
SMB unit for a oleanolic/ursolic acids mixture separation was accomplished,
with a 2-2-2-2 con guration, using the packing of Acclaim C30 column and
methanol/water 95/5 (%, v/v) as mobile phase and the equilibrium and
kinetic parameters previously optimized. The obtained results for con guration
showed that the separation of oleanolic and ursolic acids is viable, being
possible to produce puri ed streams of each acid with concentrations above
99%.<br>No último meio século, os produtos naturais tem sido gradualmente esquecidos
pelas maiores companhias de investigação farmacêuticas como fontes
de novos medicamentos. Porém atualmente observou-se uma mudança de
estratégia que promoveu um novo interesse na integração dos produtos naturais
nestes. Os ácidos triterpénicos fazem parte de um grupo promissor
de metabolitos secundários que podem ser encontrados em plantas, flores,
folhas com grande variedade de benficios. Nesta dissertação estudou-se o isolamento
dos ácidos oleanólico e ursólico por técnicas cromatográficas, recorrendo
à tecnologia de leito móvel simulado (SMB). A separação dos ácidos
triterpénicos é complexa dada à sua semelhança estrutural. No laboratório
do grupo EgiChem (CICECO, UA) está a ser instalada uma unidade de
SMB, porém é necessário realizar ensaios preliminares para selecionar a fase
móvel mais adequada, bem como a otimização das condições de operação
de ambos os ácidos. Posto isto, analisou-se a influência do solvente/mistura
de solventes na separação cromatográfica recorrendo à medição de seletividades,
factores de retenção e resolução. Foi testada uma coluna triacontyl
(Acclaim C30), sendo que os melhores resultados foram obtidos com a fase
móvel metanol/água 95/5 (%, v/v). Seguiu-se à medição das curvas de rutura
de modo a determinar os parametros de processo como as isotérmicas
de equilibrio (constantes Hi) do ácido oleanólico e do ácido ursólico, e os
coeficientes globais de transferência de massa do modelo da força motriz
linear (KLDF,i). Os dados obtidos foram modelados através da equação de
Klinkenberg, que é aplicado especificamente para sistemas lineares. As constantes
de equilíbrio linear dos ácidos oleanólico e ursólico foram HOA= 2.06
e HUA= 2.16, respetivamente, e os coeficientes globais de transferência de
massa LDF de KLDF,OA= 30.48 min-1 e KLDF,UA= 101.45 min-1 com desvios
médios de AADROA=18.29 % e AADRUA=26.88 %. Por fim foi realizada
uma simulação de uma unidade SMB para a separação de uma mistura de
ácidos oleanólico e ursólico, com uma configuração 2-2-2-2, usando o enchimento
da coluna Acclaim C30 e a fase móvel metanol/água 95/5 (%, v/v)
e os parâmetros de equilibrio e cinética otimizados anteriormente. Os resultados
obtidos para esta configuração mostraram que é possível produzir
correntes purificadas de cada ácido com concentrações acima dos 99%.<br>Mestrado em Engenharia Química
Book chapters on the topic "Bioactive triterpene"
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Yoshikawa, Masayuki, Toshiyuki Murakami, Yuhao Li, Hiromi Shimada, Johji Yamahara, and Hisashi Matsuda. "Bioactive triterpene glycosedes from several medicinal foodstuffs." In Advances in Plant Glycosides, Chemistry and Biology, Proceedings of the International Symposium in Plant Glycosides. Elsevier, 1999. http://dx.doi.org/10.1016/s0928-3420(99)80005-6.
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Chludil, Hugo D., Ana P. Murray, Alicia M. Seldes, and Marta S. Maier. "Biologically Active Triterpene Glycosides from Sea Cucumbers (Holothuroidea, Echinodermata)." In Bioactive Natural Products (Part I). Elsevier, 2003. http://dx.doi.org/10.1016/s1572-5995(03)80150-3.
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Kalinin, Vladimir I., Dmitry L. Aminin, Sergey A. Avilov, Alexandra S. Silchenko, and Valentin A. Stonik. "Triterpene Glycosides from Sea Cucucmbers (Holothurioidea, Echinodermata). Biological Activities and Functions." In Bioactive Natural Products (Part O). Elsevier, 2008. http://dx.doi.org/10.1016/s1572-5995(08)80006-3.
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Alvarenga, Nelson, and Esteban A. Ferro. "Bioactive Triterpenes and Related Compounds from Celastraceae." In Bioactive Natural Products (Part K). Elsevier, 2005. http://dx.doi.org/10.1016/s1572-5995(05)80044-4.
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"Effects of Pentacyclic Triterpenes from Olives on Colon Cancer." In Bioactive Foods and Extracts. CRC Press, 2010. http://dx.doi.org/10.1201/b10330-33.
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Alvarenga, Nelson, and Esteban A. Ferro. "Bioactive Triterpenes and Related Compounds from Celastraceae." In Studies in Natural Products Chemistry. Elsevier, 2006. http://dx.doi.org/10.1016/s1572-5995(06)80029-3.
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Menaa, F., S. L. Badole, B. Menaa, A. Menaa, and S. L. Bodhankar. "Anti-Inflammatory Benefits of Pentacyclic Triterpenes." In Bioactive Food as Dietary Interventions for Arthritis and Related Inflammatory Diseases. Elsevier, 2013. http://dx.doi.org/10.1016/b978-0-12-397156-2.00030-2.
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Valencia, Alexandra, Frank L. Romero-Orejon, Adriana Viñas-Ospino, Dayana Barriga-Rodriguez, Ana María Muñoz, and Fernando Ramos-Escudero. "Sacha Inchi Seed (Plukenetia volubilis L.) Oil: Terpenoids." In Terpenes and Terpenoids - Recent Advances. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96690.
Full textAbstract:
Sacha inchi oil is a product obtained from oilseed (Plukenetia volubilis L.) and is an excellent source of bioactive compounds, especially in polyunsaturated fatty acids, tocopherols, and sterols. These compounds are causally related to their positive impact on human health. In this study summarizes some monoterpenes, sesquiterpenes, and triterpenes reported in Sacha inchi oil seeds and reviews their sensory properties. The terpenoids that characterize Sacha inchi seed oil are: α-pinene, sabinene, limonene, aristolene, cycloartenol, 24-methylene cycloartenol, lanosterol, β-sitosterol, stigmasterol, campesterol and phytol. The sensory properties of this oil are due to a set of volatile compounds including terpenoids, the odor descriptors of monoterpenes, sesquiterpenes and diterpenes are: flower, pine, turpentine, pepper, wood, lemon, orange, and sweet. These compounds were characterized by gas chromatography with different detectors.
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Tung, Bui Thanh. "Medicinal Plants and Their Bioactive Compounds for the Management and Treatment of Gout." In Advances in Medical Diagnosis, Treatment, and Care. IGI Global, 2021. http://dx.doi.org/10.4018/978-1-7998-4453-2.ch012.
Full textAbstract:
Gout is a complex form of arthritis which is characterized by sudden, severe attacks of pain, swelling, redness, and tenderness in the joints. Gout is a type of inflammatory arthritis that is triggered by the crystallization of uric acid within the joints and is often associated with hyperuricemia. Natural products offer many options to reduce the progress and symptoms of diseases, including gout. Natural compound structure including lignans, flavonoids, tannins, polyphenols, triterpenes, sterols, and alkaloids have anti-inflammatory, antioxidant, and XO activities. In this chapter, the authors present medicinal plants and isolated compounds which are used to prevent and reduce the pathogenesis of gout.
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Eryılmaz Pehlivan, Fadime. "Bitter Melon: A Multifunctional Medicinal Plant with Powerful Bioactive Compounds." In Functional Foods [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.98812.
Full textAbstract:
Nature is full of poisons as well as life-saving entities. Extracts of natural products in medicinal plants have been used for thousands of years in traditional medicine throughout the World. Bitter melon (Momordica charantia) is a member of Cucurbitaceae family, widely distributed in tropical regions of the World, that has been used in folk medicine for the treatment of diabetes mellitus, and its fruit has been used as a vegetable for thousands of years. It contains phytochemicals, flavonoids, triterpenes, saponins, ascorbic acid, steroids, proteins, and polysaccharides. This plant is a traditional herbal medicine, possesses various biological, medicinal activities and pharmacological functions, namely antidiabetic, anthelmintic, contraceptive, antimalarial, laxative, antihyperglycemic, antimutagenic, antiulcer, antilipolytic, antifertility, hepatoprotective, anticancer, antibacterial, antiviral, antitumor, immunomodulation, antioxidant, antidiabetic, and anti-inflammatory activities of M. charantia have been reported. Its fruit has a special bitter taste, parts of M. charantia, such as fruits, vines, leaves and even roots have been used as folk medicine for the remedy of diseases like toothache, diarrhea, and diabetes. It is also used for the treatment of eczema, gout, jaundice, pneumonia, psoriasis, and rheumatism. These beneficial effects are attributed to the various bioactive components of M. charantia, which are important sources of phytoconstituents used to treat various diseases since ancient times. This chapter reviews various aspects of the results of investigations involving M. charantia in the recent years, providing a comprehensive overview of the phytochemical application of M. charantia to attract more attention to their biological activities for better utilization of M. charantia; focusing on the review of benefits that bitter melon offers in terms of its potential as a source of bioactive compounds and its role in the control of different diseases.