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Dissertations / Theses on the topic 'Bioassay-guided isolation'

Author: Grafiati
Published: 4 June 2021
Last updated: 26 July 2025

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1

Furbacher, Todd Raymond. "Bioassay-guided isolation of potential antineoplastic natural products from Southwestern plants." Diss., The University of Arizona, 2001. http://hdl.handle.net/10150/279927.

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This dissertation details the investigation of numerous plants for potential antineoplastic compounds. 144 plants (391 extracts) were prescreened with an assortment of assays. The pre-screens included an Agrobacterium tumefaciens/potato disk gall tumor inhibition assay, a Saccharomyces cerevisiae mutant topoisomerase assay, and an Escherichia coli plasmid scission assay. Bioassay-guided fractionation was conducted on three plants, Phoradendron juniperinum, Psorothamnus thompsoniae , and Acourtia thurberi, using a different assay for each. Phoradendron juniperinum (Viscaceae) was screened with
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2

Mianda, Mutombo Sephora. "Isolation of antiplasmodial naphthylisoquinoline alkaloids from Ancistrocladus sp. through bioassay-guided fractionation." Diss., University of Pretoria, 2019. http://hdl.handle.net/2263/77853.

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Ancistrocladus is the only genus in the Ancistrocladaceae family and together with the closely related Dioncophyllaceae, they produce naphthylisoquinoline (NIQ) alkaloids. This class of compounds exhibits a very interesting large spectrum of bioactivities. Some of these compounds have shown very promising antiplasmodial activity. Leaves of an as yet unidentified Ancistrocladus sp collected in the region of Bonsolerive (Northwestern part of DRC), were investigated for their antiplasmodial activity. They were air dried at room temperature and ground. The ground plant material was extracted seque
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3

Laidlay, Sharada. "Bioassay-guided isolation and biochemical characterisation of vasorelaxant compounds extracted from a Dalbergia species." Thesis, University of Brighton, 2016. https://research.brighton.ac.uk/en/studentTheses/90f9b2b4-e0bd-4872-902f-598bc357c02c.

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Natural products have been the source of many of our successful drugs providing us with an unrivalled chemical diversity combined with drug-like properties. The search for bioactive compounds can be helped considerably by the phytotherapeutic knowledge held by indigenous communities. In this study solvent extracts from the bark of a Dalbergia species and used by a community in Borneo, will be investigated to isolate, identify and biochemically characterise compounds showing vasorelaxation. At the core of this study is the hypertensive model, which uses phenylephrine precontracted rat aortic ri
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4

Dai, Yumin. "Isolation, Synthesis and Structure-Activity Relationship Study of Anticancer and Antimalarial Agents from Natural Products." Diss., Virginia Tech, 2013. http://hdl.handle.net/10919/24193.

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The Kingston group's engagement in an International Cooperative Biodiversity Group (ICBG) program and a collaborative research project established between Virginia Tech and the Institute for Hepatitis and Virus Research (IHVR) has focused on the search for bioactive natural products from tropical forests in both Madagascar and South Africa. As a part of this research, a total of four antiproliferative extracts were studied, leading to the isolation of fourteen novel compounds with antiproliferative activity against the A2780 human ovarian cancer line. One extract with antimalarial activity was
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5

Sjögren, Jörgen. "Bioassay-guided isolation and characterisation of antifungal metabolites : studies of lactic acid bacteria and propionic acid bacteria /." Uppsala : Dept. of Chemistry, Swedish University of Agricultural Sciences, 2005. http://epsilon.slu.se/200517.pdf.

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6

Ta, Chieu Anh Kim. "Bacterial Biofilm Inhibition and Antifungal Activity of Neotropical Plants." Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/32419.

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This thesis examined the antimicrobial activity of select neotropical plants from Costa Rica and traditional Q’eqchi Maya medicines from Belize. In particular the potential for interference with bacterial quorum sensing (QS) and biofilm formation as well as fungal growth were assessed. Of one hundred and twenty six extracts collected from Costa Rica, one third showed significant QS inhibition while 13 species displayed more biofilm inhibitory activities than the positive control allicin. The active species belonged to the Lepidobotryaceae, Melastomataceae, Meliaceae, Sapindaceae, and Simarouba
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7

Afonso, Tiago. "Bioassay-guided isolation of cyanobacterial metabolites with anticancer activity." Dissertação, 2014. http://hdl.handle.net/10216/77645.

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8

Afonso, Tiago André Barros. "Bioassay-guided isolation of cyanobacterial metabolites with anticancer activity." Master's thesis, 2014. https://repositorio-aberto.up.pt/handle/10216/83654.

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9

Afonso, Tiago André Barros. "Bioassay-guided isolation of cyanobacterial metabolites with anticancer activity." Dissertação, 2014. https://repositorio-aberto.up.pt/handle/10216/83654.

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10

Hung, Ya-Ling, and 洪雅菱. "Bioassay-guided isolation and identification of anti-inflammatory compounds from wild bitter melon leaf against Porphyromonas gingivalis." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/758365.

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11

Huang, Wen-Cheng, and 黃文程. "Bioassay-guided isolation and identification of anti-inflammatory compounds from wild bitter melon leaf against Propionibacterium acnes." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/29vd6a.

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12

Chang, Yu-Hsiu, and 張羽秀. "Bioassay-guided Isolation of Active Components from the Fruit of Canarium album toward Inhibition of Cancer Cell Growth." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/37127535002346538077.

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碩士<br>國立臺灣大學<br>食品科技研究所<br>102<br>Chinese olive (Canarium album.), which belongs to family of Burseraceae and genus of Canarium, is a traditional Chinese medicine with antibiotic, anti-virus, anti-inflammatory, as well as detoxification effects, and is used for curing infection, malaria, venom poinsoning. Former results in our lab indicated that the methanol-butanol olive extraction (MBO) significantly repressed the viability of murine colon cancer cells (CT26); however, more investigations for verifying the bioactive compounds in human cells model are needed. In the present study, Chinese oli
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13

"Bioassay-guided isolation, characterization and mechanistic study of the bioactive components from Sophora flavescens for the anti-proliferative effect on human hepatoma cells." 2006. http://library.cuhk.edu.hk/record=b5892898.

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by Tsang Kit Man.<br>Thesis (M.Phil.)--Chinese University of Hong Kong, 2006.<br>Includes bibliographical references (leaves 179-188).<br>Abstracts in English and Chinese.<br>ABSTRACT --- p.i<br>ABSTRACT IN CHINESE (摘要） --- p.iii<br>ACKNOWLEDGEMENTS --- p.v<br>CONTENTS --- p.vi<br>LIST OF FIGURES --- p.xi<br>LIST OF TABLES --- p.xiv<br>ABBREVIATIONS --- p.xvi<br>Chapter CHAPTER ONE: --- INTRODUCTION --- p.1<br>Chapter 1.1 --- Hepatocellular Carcinoma --- p.2<br>Chapter 1.1.1 --- Incidence of Hepatocellular Carcinoma --- p.2<br>Chapter 1.1.2 --- Therapies for Hepatocellular Carcinoma
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14

"Bioassay-guided isolation, characterization and mechanistic study of bioactive components from oldenlandia diffusa and androsace umbellata for anti-proliferative effect on human hepatoma cells." Thesis, 2007. http://library.cuhk.edu.hk/record=b6074511.

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Eleven known compounds were separated from Oldenlandia diffusa using the bioassay-guided methods. Among which, heptatriacontane and stearic acid (SA) were isolated from this herb for the first time. The anti-proliferative activities of ursolic acid (UA) and SA, as well as the anti-proliferative and immunomodulatory activities of quercetin, kaempferol, quercetin-3-O-D-glucoside, kaempferol-3-O-D-glucoside and kaempferol-3-O-D-galactoside, are responsible for the anti-hepatomatic effect of OD, to which UA might be the major contributor due to relatively high content in OD and potent cytotoxicit
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15

"Bioassay-guided isolation, characterization, and mechanistic study of the bioactive components from scutellaria barbata for the anti-proliferative effect on human hepatoma cells in vitro adn in vivo." Thesis, 2007. http://library.cuhk.edu.hk/record=b6074509.

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Both mRNA and protein expression levels of P-glycoprotein, one of the major factors involved in drug resistance, was decreased in Pa-treated R-HepG2 cells. The chemo-sensitivity of these MDR cells towards doxorubicin would be enhanced by pretreatment of Pa.<br>In the study, 35 TCMs with historical background in treating liver diseases were screened. S. barbata was chosen for intensive studies based on its significant anti-hepatoma activity. Using bioassay-guided purification approach, an active component, pheophorbide a (Pa) - a chlorophyll derivative, was isolated from Scutellaria barbata.<br
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