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Journal articles on the topic 'Bioassay-guided isolation'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Pesca, Maria S., Fabrizio Dal Piaz, Rokia Sanogo, et al. "Bioassay-Guided Isolation of Proanthocyanidins with Antiangiogenic Activities." Journal of Natural Products 76, no. 1 (2012): 29–35. http://dx.doi.org/10.1021/np300614u.

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2

Rauf, Abdur, Ghias Uddin, Haroon Khan, Mohammad Arfan, and Bina S. Siddiqui. "Bioassay-guided isolation of antibacterial constituents fromDiospyros lotusroots." Natural Product Research 30, no. 4 (2015): 426–28. http://dx.doi.org/10.1080/14786419.2015.1013957.

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3

Dogan, Z., VM Kutluay, and I. Saracoglu. "Bioassay-guided isolation studies on Scutellaria salviifolia Benth." Planta Medica 81, S 01 (2016): S1—S381. http://dx.doi.org/10.1055/s-0036-1596613.

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4

Wang, Yu, Tongle Deng, Lin Lin, Yuanjiang Pan, and Xiaoxiang Zheng. "Bioassay-guided isolation of antiatherosclerotic phytochemicals fromArtocarpus altilis." Phytotherapy Research 20, no. 12 (2006): 1052–55. http://dx.doi.org/10.1002/ptr.1990.

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5

Emile, Aurélie, Jean Waikedre, Christine Herrenknecht, et al. "Bioassay-guided isolation of antifungal alkaloids fromMelochia odorata." Phytotherapy Research 21, no. 4 (2007): 398–400. http://dx.doi.org/10.1002/ptr.2078.

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6

Yin, Yihao, Jiehui Li, Qi Cao, et al. "Bioassay-guided isolation of terpenoids from Rubus rosifolius." Phytochemistry Letters 65 (February 2025): 124–27. https://doi.org/10.1016/j.phytol.2025.01.006.

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7

Rodriguez-Sanchez, Dariana Graciela, Mirthala Flores-García, Christian Silva-Platas, et al. "Isolation and chemical identification of lipid derivatives from avocado (Persea americana) pulp with antiplatelet and antithrombotic activities." Food & Function 6, no. 1 (2015): 192–202. http://dx.doi.org/10.1039/c4fo00610k.

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8

Zhang, Xing, Fei Qian, Jun-Jie Tan, et al. "Bioassay-guided isolation of bisepoxylignans from the flower buds of Magnolia biondii Pamp and their antiallergic effects." RSC Advances 7, no. 54 (2017): 34236–43. http://dx.doi.org/10.1039/c7ra01476g.

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9

Niu, Siwen, Dong Liu, Zongze Shao, Peter Proksch, and Wenhan Lin. "Eutypellazines A–M, thiodiketopiperazine-type alkaloids from deep sea derived fungus Eutypella sp. MCCC 3A00281." RSC Advances 7, no. 53 (2017): 33580–90. http://dx.doi.org/10.1039/c7ra05774a.

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10

da Cruz-Silva, Claudia T. A., Charles L. Cantrell, Lucia H. P. Nóbrega, Abbas Ali, and Stephen O. Duke. "Bioassay-guided isolation of phytotoxins from three Salvia species." Allelopathy Journal 54, no. 1 (2021): 13–24. http://dx.doi.org/10.26651/allelo.j/2021-54-1-1344.

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11

Oluyemisi, Ogbole Omonike, Ajaiyeoba Edith Oriabure, Adeniji J. Adekunle, Kamdem Soup T. Ramsay, Sajan Shyyaula, and Muhammad Iqbal Choudhary. "Bioassay-guided isolation of Poliovirus-inhibiting constituents fromZephyranthes candida." Pharmaceutical Biology 53, no. 6 (2014): 882–87. http://dx.doi.org/10.3109/13880209.2014.946061.

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12

Zucolotto, Silvana, Stella Goulart, Ana Montanher, Flávio Reginatto, Eloir Schenkel, and Tânia Fröde. "Bioassay-Guided Isolation of Anti-InflammatoryC-Glucosylflavones fromPassiflora edulis." Planta Medica 75, no. 11 (2009): 1221–26. http://dx.doi.org/10.1055/s-0029-1185536.

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13

Meickle, Theresa, Susan Matthew, Cliff Ross, Hendrik Luesch, and Valerie Paul. "Bioassay-Guided Isolation and Identification of Desacetylmicrocolin B fromLyngbyacf.polychroa." Planta Medica 75, no. 13 (2009): 1427–30. http://dx.doi.org/10.1055/s-0029-1185675.

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14

Li, Yuan-Yuan, Xu-Li Tang, Tao Jiang, Pei-Fen Li, Ping-Lin Li, and Guo-Qiang Li. "Bioassay-guided isolation of neo-clerodane diterpenoids fromScutellaria barbata." Journal of Asian Natural Products Research 15, no. 9 (2013): 941–49. http://dx.doi.org/10.1080/10286020.2013.821983.

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15

Rauf, Abdur, Ghias Uddin, Noor Jehan, et al. "Bioassay-Guided Isolation of Antioxidants from Diospyros lotus Roots." Chemistry of Natural Compounds 53, no. 5 (2017): 946–48. http://dx.doi.org/10.1007/s10600-017-2164-3.

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16

Wang, Ying-Ying, Shu-Ning Zheng, Ting Tian, et al. "Bioassay-Guided Isolation of Cytotoxic Steroids from Neosartorya fischeri." Chemistry of Natural Compounds 56, no. 1 (2020): 173–76. http://dx.doi.org/10.1007/s10600-020-02978-4.

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17

Zhou, Yajuan, Srijan Shrestha, Hyun Ah Jung, and Jae Sue Choi. "Bioassay-guided isolation of BACE1 inhibitors from Crataegus pinnatifida." Phytomedicine Plus 3, no. 3 (2023): 100460. http://dx.doi.org/10.1016/j.phyplu.2023.100460.

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18

Li, Yong, Dong Liu, Zhongbin Cheng, Peter Proksch, and Wenhan Lin. "Cytotoxic trichothecene-type sesquiterpenes from the sponge-derived fungus Stachybotrys chartarum with tyrosine kinase inhibition." RSC Advances 7, no. 12 (2017): 7259–67. http://dx.doi.org/10.1039/c6ra26956g.

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19

López-Ogalla, Javier, Esther García-Palomero, Jorge Sánchez-Quesada, et al. "Bioactive prenylated phenyl derivatives derived from marine natural products: novel scaffolds for the design of BACE inhibitors." MedChemComm 5, no. 4 (2014): 474–88. http://dx.doi.org/10.1039/c3md00236e.

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Bioassay guided-fractionation of active extracts from the sponge Sarcotragus sp. led to the isolation biochemical characterization and development of a chemistry program of bioactive prenylated phenyl derivatives.

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20

Tabudravu, J. N., and M. Jaspars. "Anticancer activities of constituents of kava (Piper methysticum)." South Pacific Journal of Natural and Applied Sciences 23, no. 1 (2005): 26. http://dx.doi.org/10.1071/sp05005.

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Crude extracts of kava (Piper methysticum G. Forster, Piperaceae) showed good activity against ovarian tumour and leukaemia cancer cell lines. Bioassay-guided isolation resulted in the isolation of six known kava lactones and two flavokavains. The structure of the compounds were elucidated by spectroscopic techniques and by comparison with data in the literature.

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21

Renda,, Gülin, Arzu Özel, Burak Barut, et al. "Bioassay Guided Isolation of Active Compounds from Alchemilla barbatiflora Juz." Records of Natural Products 12, no. 1 (2017): 76–85. http://dx.doi.org/10.25135/rnp.07.17.07.117.

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22

F. Haron, Farah, Kamaruzaman Sijam, Dzolkhifli Omar, and Mawardi Rahmani. "Bioassay-guided Isolation of Antifungal Plumericin from Allamanda Species (Apocynaceae)." Journal of Biological Sciences 13, no. 3 (2013): 158–62. http://dx.doi.org/10.3923/jbs.2013.158.162.

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23

Akbari, Vajihe, Mahshid Abedi, and Afsaneh Yegdaneh. "Bioassay-guided isolation of glycolipids from the seaweed Gracilaria corticata." Research in Pharmaceutical Sciences 15, no. 5 (2020): 473. http://dx.doi.org/10.4103/1735-5362.297850.

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24

Das, Jayshree, and Dhruva Jha. "Bioassay-guided Isolation of Antidermatophytic Compounds from Piper longum L." Journal of Advances in Medical and Pharmaceutical Sciences 12, no. 4 (2017): 1–7. http://dx.doi.org/10.9734/jamps/2017/31874.

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25

Zhou, Ling-Yun, Xiao-Hong Yu, Bin Lu, and Yan Hua. "Bioassay-Guided Isolation of Cytotoxic Isocryptoporic Acids from Cryptoporus volvatus." Molecules 21, no. 12 (2016): 1692. http://dx.doi.org/10.3390/molecules21121692.

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26

Aamer, Muhammad, Sadaf Iqbal, Almas Jabeen, et al. "Bioassay-Guided Isolation of Anti-Inflammatory Constituents from Erigeron canadensis." Chemistry of Natural Compounds 57, no. 4 (2021): 749–51. http://dx.doi.org/10.1007/s10600-021-03465-0.

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27

Kang, Hee Rae, Hee Jeong Eom, Seoung Rak Lee, et al. "Bioassay-guided Isolation of Antiproliferative Triterpenoids from Euonymus alatus Twigs." Natural Product Communications 10, no. 11 (2015): 1934578X1501001. http://dx.doi.org/10.1177/1934578x1501001131.

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Euonymus alatus (Celastraceae) has been used as an anticancer agent in Korean traditional medicine. However, the potential bioactive contributors to the anticancer effects have not been thoroughly studied. Our screening test revealed that the MeOH extract of E. alatus twigs exhibited significant cytotoxicity against A549, SK-OV-3, and SK-MEL-2 cell lines. A bioassay-guided separation of the MeOH extract of E. alatus twigs resulted in the isolation and identification of 14 triterpenes as main phytochemicals. The structures of the compounds were elucidated on the basis of spectroscopic evidence

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28

Alam, M., A. Khan, A. Wadood, et al. "Bioassay-guided isolation of urease inhibitors from Ferula narthex Bioss." South African Journal of Botany 120 (January 2019): 247–52. http://dx.doi.org/10.1016/j.sajb.2018.07.011.

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29

Logendra, Sithes, David M. Ribnicky, Hui Yang, et al. "Bioassay-guided isolation of aldose reductase inhibitors from Artemisia dracunculus." Phytochemistry 67, no. 14 (2006): 1539–46. http://dx.doi.org/10.1016/j.phytochem.2006.05.015.

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30

He, Yan, Zhengxi Hu, Qin Li, et al. "Bioassay-Guided Isolation of Antibacterial Metabolites from Emericella sp. TJ29." Journal of Natural Products 80, no. 9 (2017): 2399–405. http://dx.doi.org/10.1021/acs.jnatprod.7b00077.

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31

Dias Silva, Marcelo J., Ana M. Simonet, Naiara C. Silva, Amanda L. T. Dias, Wagner Vilegas, and Francisco A. Macías. "Bioassay-Guided Isolation of Fungistatic Compounds from Mimosa caesalpiniifolia Leaves." Journal of Natural Products 82, no. 6 (2019): 1496–502. http://dx.doi.org/10.1021/acs.jnatprod.8b01025.

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32

Ambali, Owoola Azeezat, Samuel Ayoolu Oguntimehin, Edith Oriabure Ajaiyeoba, Omonike Oluyemisi Ogbole, and Adekunle Johnson Adeniji. "Bioassay-guided isolation of cytotoxic constituents of Aframomum melegueta K.Schum. seeds." Nigerian Journal of Natural Products and Medicine 25, no. 1 (2021): 80–85. http://dx.doi.org/10.4314/njnpm.v25i1.9.

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The seeds of Aframomum melegueta are used extensively in the Nigerian ethnomedicine for the management of cancer. This study therefore aimed at isolating and characterizing its cytotoxic constituents. Methanol extract of the seed was obtained through cold maceration, and it was further partitioned into n – hexane, dichloromethane and ethylacetate. The most active fraction was purified on repeated chromatographic techniques, using vacuum liquid chromatography (VLC), column (CC), and high-performance liquid chromatography (HPLC). The extract, purified fractions and isolated compounds were tested

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33

Lee, Shoei-Sheng, Yue Xi, Chien-Kuang Chen, Ching-Chia Chang, and Sheng-Fa Tsai. "A Novel 3,4-Secocycloartane Triterpene, Bearing a Tetrahydropyran Moiety, from Pasania formosana Leaf." Natural Product Communications 11, no. 8 (2016): 1934578X1601100. http://dx.doi.org/10.1177/1934578x1601100811.

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Bioassay guided fractionation against α-glucosidase and separation of the ethanolic extract of Pasania formosana leaf by chromatographic methods led to the isolation of a novel secocycloartane triterpene. This compound, named pasasecocycloartenoic acid, was elucidated as 21,24( R)-epoxy-25-hydroxy-3,4-secocycloart-4(28)-en-3-oic acid through analysis of 1D and 2D NMR spectra and on the basis of HRESIMS data. The compound showed weak activity against α-glucosidase, but its poor solubility hampered the bioassay.

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34

Lau, Kit-Man, Lai-Hong Fu, Ling Cheng, et al. "Two Antifungal Components Isolated from Fructus Psoraleae and Folium Eucalypti Globuli by Bioassay-Guided Purification." American Journal of Chinese Medicine 38, no. 05 (2010): 1005–14. http://dx.doi.org/10.1142/s0192415x10008421.

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Fructus Psoraleae and Folium Eucalypti Globuli have long been used as Chinese medicines to treat various ailments such as asthma, eczema and dermatomycosis. In previous studies, their antifungal activities were demonstrated. The aim of the present study was to isolate active antidermatophytic compounds from their ethanolic extracts by means of bioassay-guided purification. Guided by the inhibitory activities on Trichophyton mentagrophytes, Trichophyton rubrum and Paecilomyces variotii, bakuchiol was isolated from the n-hexane fraction of Fructus Psoraleae whilst macrocarpal C was isolated from

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35

Clark, Trevor N., Amanda I. Bishop, Mark McLaughlin, Larry A. Calhoun, John A. Johnson, and Christopher A. Gray. "Isolation of (–)–Avenaciolide as the Antifungal and Antimycobacterial Constituent of a Seimatosporium sp. Endophyte from the Medicinal Plant Hypericum perforatum." Natural Product Communications 9, no. 10 (2014): 1934578X1400901. http://dx.doi.org/10.1177/1934578x1400901022.

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An extract of Seimatosporium sp., an endophyte from the Canadian medicinal plant Hypericum perforatum, exhibited significant antifungal and antimycobacterial activity against Candida albicans and Mycobacterium tuberculosis H37Ra. Bioassay guided fractionation led to the isolation of (-)–avenaciolide as the only bioactive constituent of the extract. This is the first report of both the antimycobacterial activity of avenaciolide and its isolation from a Seimatosporium sp. fungus.

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36

Siriwatanametanon, Nisarat, та Michael Heinrich. "The Thai Medicinal Plant Gynura Pseudochina var. hispida: Chemical Composition and in vitro NF-κB Inhibitory Activity". Natural Product Communications 6, № 5 (2011): 1934578X1100600. http://dx.doi.org/10.1177/1934578x1100600512.

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Gynura pseudochina (L.) var. hispida Thv. (Asteraceae) has been used in traditional medicine in Thailand for the treatment of conditions associated with chronic and acute inflammation. In continuation of our search for bioactive natural products from Thai medicinal plants, G. pseudochina var. hispida showed potential in vitro NF-κB (Nuclear Factor kappa B) inhibitory activity and, therefore, was chosen for bioassay-guided isolation of active compounds. Bioassay-guided fractionation of the methanol extract of the leaves of G. pseudochina var. hispida led to the isolation and identification, by

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37

B, PRABHA, BIJI M, MADHUKRISHNAN M та ін. "Bioassay-guided isolation and characterization of potential α-glucosidase inhibitors from the seeds of Quassia indica Gaetrn." Journal of Medicinal and Aromatic Plant Sciences 43, № 1 (2021): 25–36. http://dx.doi.org/10.62029/jmaps.v43i1.b.

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38

Kaur, Swayamjot, Saroj Arora, Satwinderjeet Kaur, and Subodh Kumar. "Bioassay-Guided Isolation of Antimutagenic Factors from Fruits of Terminalia Bellerica." Journal of Environmental Pathology, Toxicology and Oncology 22, no. 1 (2003): 67–76. http://dx.doi.org/10.1615/jenvpathtoxoncol.v22.i1.70.

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39

Adorisio, Sabrina, Laura Giamperi, Anahi Elena Ada Bucchini, Domenico Vittorio Delfino, and Maria Carla Marcotullio. "Bioassay-Guided Isolation of Antiproliferative Compounds from Limbarda crithmoides (L.) Dumort." Molecules 25, no. 8 (2020): 1893. http://dx.doi.org/10.3390/molecules25081893.

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Limbarda crithmoides (L.) Dumort (Asteraceae) n-hexane extract displayed high cell proliferation inhibitory activity against acute myeloid leukaemia cells (OCI-AML3) and was therefore subjected to a bioassay-guided multistep separation procedure. Two thymol derivatives, namely 10-acetoxy-8,9-epoxythymol tiglate (1) and 10-acetoxy-9-chloro-8,9-dehydrothymol (2), were isolated and identified by means of NMR spectroscopy. Both of them exhibited a significant dose-dependent inhibition of cell proliferation.

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40

Sule. "Bioassay Guided Isolation of Antibacterial Compounds from Andrographis paniculata." American Journal of Applied Sciences 8, no. 6 (2011): 525–34. http://dx.doi.org/10.3844/ajassp.2011.525.534.

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41

Sheeja, E., S. B. Joshi, and D. C. Jain. "Bioassay-guided isolation of anti-inflammatory and antinociceptive compound fromPlumbago zeylanicaleaf." Pharmaceutical Biology 48, no. 4 (2010): 381–87. http://dx.doi.org/10.3109/13880200903156424.

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42

Idid, Syed, Shahbudin Saad, and Deny Susanti. "Bioassay-Guided Isolation of Antimicrobial Compounds from Marine Sponge Neopetrosia exigua." Journal of Basic and Applied Research in Biomedicine 10, no. 1 (2024): 50–58. https://doi.org/10.51152/jbarbiomed.v10i1.235.

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The marine sponge Neopetrosia exigua has shown great potential as a source of bioactive compounds with significant antimicrobial properties. This study applied bioassay-guided fractionation to isolate and evaluate the antimicrobial activities of various compounds from N. exigua collected off the coast of Langkawi Island, Malaysia. Methanol extracts were partitioned into different fractions, with dichloromethane (CH₂Cl₂) and n-butanol (n-BuOH) fractions exhibiting the most potent antimicrobial activities. Four compounds were isolated: a previously undescribed bisulphate avarol derivative (1), t

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43

Amico, Vincenzo, Vincenza Barresi, Rosa Chillemi, et al. "Bioassay-Guided Isolation of Antiproliferative Compounds from Grape (Vitis vinifera) Stems." Natural Product Communications 4, no. 1 (2009): 1934578X0900400. http://dx.doi.org/10.1177/1934578x0900400108.

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The fractionation, guided by cell-growth inhibition assay, of the EtOAc crude extract from grape stems of the Sicilian Vitis vinifera variety ‘Nerello Mascalese’ allowed identification of ten constituents, isolated either as pure compounds (1, 3–5, 7–10) or inseparable mixtures (2a-d and 6a-e). The pure constituents were: two triterpenoid acids, oleanolic (1) and betulinic acids (5); daucosterol (7); a stilbenoid, E-resveratrol (3) and its dimer E-ε-viniferin (4); the simple phenol gallic acid (8); and the flavanols catechin (9) and gallocatechin (10). Four 6′- O-acyldaucosterols (2a-d) and fi

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44

Huang, Sheng-Xiong, Chao Feng, Yan Zhou, et al. "Bioassay-Guided Isolation of Xanthones and Polycyclic Prenylated Acylphloroglucinols fromGarcinia oblongifolia." Journal of Natural Products 72, no. 1 (2009): 130–35. http://dx.doi.org/10.1021/np800496c.

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45

Gan, M. L., L. F. Han, R. F. Wang, and Z. C. Yang. "Bioassay-guided isolation of an antimycobacterial compound from Leonurus japonicus Houtt." South African Journal of Botany 121 (March 2019): 92–97. http://dx.doi.org/10.1016/j.sajb.2018.10.025.

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46

Wang, Meng-Yue, Ying Peng, Chong-Sheng Peng, Jiang-Yuan Qu, and Xiao-Bo Li. "The bioassay-guided isolation of antifungal saponins from Hosta plantaginea leaves." Journal of Asian Natural Products Research 20, no. 6 (2017): 501–9. http://dx.doi.org/10.1080/10286020.2017.1329304.

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47

Ayaz, Fatma, Nurgün Küçükboyacı, Nezhun Gören, et al. "Bioassay-guided isolation of cytotoxic compounds from Chrysophthalmum montanum (DC.) Boiss." Food and Chemical Toxicology 125 (March 2019): 10–20. http://dx.doi.org/10.1016/j.fct.2018.12.029.

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48

Ihtesham, Y., Z. Dogan, VM Kutluay, U. Khan, and I. Saracoglu. "Bioassay guided isolation studies on Incarvillea emodi (Royle ex Lindl.) Chatterjee." Planta Medica 81, S 01 (2016): S1—S381. http://dx.doi.org/10.1055/s-0036-1596576.

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49

Tang, Yun, Wei Li, Jiaqing Cao, Wei Li, and Yuqing Zhao. "Bioassay-guided isolation and identification of cytotoxic compounds from Bolbostemma paniculatum." Journal of Ethnopharmacology 169 (July 2015): 18–23. http://dx.doi.org/10.1016/j.jep.2015.04.003.

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50

Senejoux, François, Céline Demougeot, Parhat Kerram, et al. "Bioassay-guided isolation of vasorelaxant compounds from Ziziphora clinopodioides Lam. (Lamiaceae)." Fitoterapia 83, no. 2 (2012): 377–82. http://dx.doi.org/10.1016/j.fitote.2011.11.023.

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