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1

Khodadadi Tirkolaei, Hamed, Neda Javadi, Vinay Krishnan, Nasser Hamdan, and Edward Kavazanjian. "Crude Urease Extract for Biocementation." Journal of Materials in Civil Engineering 32, no. 12 (December 2020): 04020374. http://dx.doi.org/10.1061/(asce)mt.1943-5533.0003466.

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2

Muhammed, Abubakar Sadiq, Khairul Anuar Kassim, Muttaqa Uba Zango, Kamarudin Ahmad, and Jodin Makinda. "Effect of Palm Oil Fuel Ash on the Strength and Ammonium By-Product Removal of Biocemented Sandy Soil." Materials Science Forum 1030 (May 2021): 103–9. http://dx.doi.org/10.4028/www.scientific.net/msf.1030.103.

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Enzyme induced calcite precipitation (EICP) or biocementation has rapidly evolved in the last decade as an environmentally friendly ground improvement technique. In EICP, plant-derived urease enzyme is used to trigger the hydrolysis of urea in the presence of calcium ions to produce calcium carbonate (CaCO3) precipitate within the soil matrix. Despite the advancement in soil improvement technology via biocementation, there are still concerns about the fate of the ammonium produced as one of the by-products. Therefore, this study performed an experimental investigation to ascertain that using palm oil fuel ash (POFA) might reduce the amount of ammonia produced as a result of biocementation. The soil was mixed with POFA at different percentages (1, 2, 3, 4 and 5%) by dry weight of the soil. The effectiveness of the treatment process was evaluated by conducting the unconfined compressive strength (UCS) and the ammonium removal efficiency. Results show that the strength and ammonium removal efficiency of the biocemented soil decreased and increased, respectively, with an increase in the percentage of POFA. The highest UCS of 161 kPa was obtained at 3% POFA content, while the lowest concentration of ammonium of 0.71 mg/L was at 5% POFA content.
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3

Safdar, M. U., M. Mavroulidou, M. J. Gunn, D. Purchase, I. Payne, and J. Garelick. "Electrokinetic biocementation of an organic soil." Sustainable Chemistry and Pharmacy 21 (June 2021): 100405. http://dx.doi.org/10.1016/j.scp.2021.100405.

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4

Sharma, Meghna, Neelima Satyam, and Krishna R. Reddy. "Hybrid bacteria mediated cemented sand: Microcharacterization, permeability, strength, shear wave velocity, stress-strain, and durability." International Journal of Damage Mechanics 30, no. 4 (January 28, 2021): 618–45. http://dx.doi.org/10.1177/1056789521991196.

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Microbially induced calcite precipitation (MICP), a sustainable approach for sand biocementation, was investigated in previous studies based on metabolic activity of individual microorganisms. The individual bacteria, specifically Sporosarcina pasteurii (SP), Bacillus subtilis (BS), and Lysinibacillus sphaericus (LS), were found capable enough for sand biocementation. However, present study investigates synergistic effects of using bacterial-hybrids on cementation and consequent improvement in sand properties. The SP, BS, and LS strains were used in different combinations to create bacterial-hybrids and applied under simulated non-sterile field conditions. Initially, sand biotreatment was carried out in plastic tubes up to 14 days, using bacterial mixtures and 0.5 M cementation solution. Biocemented specimens were tested for calcite precipitation, XRD, FTIR, and SEM. The SP and LS combination (SPLS hybrid) showed maximum calcite precipitation, which is further used for biotreatment to create cylindrical sand samples for testing improved engineering properties. These samples were prepared using 0.5 M cementation solution in three pore volumes (1, 0.75, and 0.5 PV) and treatment cycles (12, 24, and 48 hrs TC) up to 18 days. Biocemented samples were tested for permeability (6th, 12th, and 18th days of biotreatment), unconfined compressive strength (UCS), split tensile strength (STS), ultrasonic pulse velocity (UPV), and consolidated undrained stress-strain response. Durability of biocementation was also investigated by determining reduction in strength and UPV subjected to freeze-thaw (FT) cycles (5, 10, 15, and 20). The results showed maximum UCS of 1902 kPa, STS of 356 kPa, UPV of 2408 m/s, and coefficient of permeability reduction up to 91%. The higher results were achieved with 11.11% calcite content in 1PV-12TC treated samples. The 1PV-12TC treated samples resulted in 4.2%, 8.3%, 17%, and 35% reduction of strength after 5, 10, 15, and 20 FT cycles, respectively. Overall, biocementation using hybrid bacteria is shown significant to improve sand's engineering properties, including potential to mitigate liquefaction.
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5

Dzulkifli, NA, RC Omar, Fathoni Usman, Hairin Taha, and KA Sanusi. "Compressive Strength of Vege-Grout Bricks." International Journal of Engineering & Technology 7, no. 4.35 (November 30, 2018): 516. http://dx.doi.org/10.14419/ijet.v7i4.35.22902.

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Brick is one of largest material used in construction of infrastructure all over the world. A conventional bricks such as clay brick and concrete brick are produced from clay with high temperature kiln firing and from ordinary Portland cement (OPC) concrete respectively. Both of this activities lead to CO2 emission. The burning process requires high temperature at the same time release carbon dioxide and pollute the environment. At present, carbon emissions has become a crucial issues in the society that must be solved. Several studies had demonstrated that brick can be produced from bacteria based on Microbial Induced Calcite Precipitation (MICP). The objective of this study is to develop cement free- brick from vegetables waste with added eggshell as calcium additive to induce biocementation of brick. Brick specimen was cast in the mould size 210 x 90 x 65 mm and casting for 28 days. The study showed that there was an increased in compressive strength up to 0.062 N/mm2 as the curing period increased to 28 days which showed the occurrence of biocementation activities. SEM-EDX analysis confirmed the presence of calcite precipitation. The result indicated that vege-grout can be used as binding agent for biocementation to produce bricks.
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6

Xiao, Peng, Hanlong Liu, Armin W. Stuedlein, T. Matthew Evans, and Yang Xiao. "Effect of relative density and biocementation on cyclic response of calcareous sand." Canadian Geotechnical Journal 56, no. 12 (December 2019): 1849–62. http://dx.doi.org/10.1139/cgj-2018-0573.

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Microbial-induced calcium carbonate precipitation (MICP) represents a promising approach to improve the geotechnical engineering properties of soils through the precipitation of calcium carbonate (CaCO3) at soil particle contacts and soil particle surfaces. An extensive experimental study was undertaken to investigate the influence of initial relative density on the efficiency of the biocementation process, the reduction of liquefaction susceptibility, and the cyclic response in biocemented calcareous soils. For this purpose, stress-controlled undrained cyclic triaxial shear (CTS) tests were carried out on untreated and MICP-treated calcareous sand specimens for different initial relative densities and magnitudes of biocementation. Improvement in the cyclic response was quantified and compared in terms of excess pore pressure generation, evolution of axial strains, and the number of cycles to liquefaction. The cyclic experiments show that MICP treatment can change the liquefaction failure mechanism from flow failure to cyclic mobility and can significantly change the excess pore pressure generation response of initially loose specimens. Scanning electron microscope (SEM) images indicate the CaCO3 crystals alter the characteristics of the sand particles and confirm the physical change in soil fabric that impacts the dynamic behavior and liquefaction resistance of MICP-treated specimens. Furthermore, the effect of biocementation was contrasted against the effect of relative density alone, and MICP treatment was shown to exhibit greater efficiency in improving the cyclic resistance than densification.
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7

Udymovych, V. "General characteristic of biocementation and control parameters." Scientific Works of National University of Food Technologies 27, no. 6 (December 2021): 30–42. http://dx.doi.org/10.24263/2225-2924-2021-27-6-5.

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8

Jaya Sri, V., and Anuja Charpe. "Strength Enhancement of Mortar using Biocementation Technique." IOP Conference Series: Earth and Environmental Science 982, no. 1 (March 1, 2022): 012028. http://dx.doi.org/10.1088/1755-1315/982/1/012028.

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Abstract Biocementation is a technique in which calcium carbonate (CaCO3) gets deposited with the help of ureolytic bacteria. In this study, an attempt has been made to augment the properties of mortar utilizing bacterial solution made with soil bacteria and other nutrients. The consequence of bacterial solution was noticed in two methods. In the first method the bacterial solution is used in preparing the bacterial mortar specimens and in the second method the bacterial solution is used in surface treatment of mortar. Considerable enhancement of 20.08% in compressive strength and 10.52% decrement in water absorption of bacterial mortar over conventional mortar was observed at the end of 56 days of curing period.
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9

Fattahi, Seyed Mohammad, Abbas Soroush, and Ning Huang. "Biocementation Control of Sand against Wind Erosion." Journal of Geotechnical and Geoenvironmental Engineering 146, no. 6 (June 2020): 04020045. http://dx.doi.org/10.1061/(asce)gt.1943-5606.0002268.

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10

Cardoso, Rafaela, Inês Pires, Sofia O. D. Duarte, and Gabriel A. Monteiro. "Effects of clay's chemical interactions on biocementation." Applied Clay Science 156 (May 2018): 96–103. http://dx.doi.org/10.1016/j.clay.2018.01.035.

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11

Cardoso, Rafaela, Rita Pedreira, Sofia O. D. Duarte, and Gabriel A. Monteiro. "About calcium carbonate precipitation on sand biocementation." Engineering Geology 271 (June 2020): 105612. http://dx.doi.org/10.1016/j.enggeo.2020.105612.

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12

Sun, Xiaohao, Linchang Miao, Linyu Wu, and Chengcheng Wang. "Study of magnesium precipitation based on biocementation." Marine Georesources & Geotechnology 37, no. 10 (January 29, 2019): 1257–66. http://dx.doi.org/10.1080/1064119x.2018.1549626.

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13

Safdar, Muhammad Umair, Maria Mavroulidou, Michael J. Gunn, Christopher Gray, Diane Purchase, Jonathan Garelick, and Ian Payne. "Implementation of biocementation for a partially saturated problematic soil of the UK railway network." E3S Web of Conferences 195 (2020): 05006. http://dx.doi.org/10.1051/e3sconf/202019505006.

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This paper refers to biocementation of a problematic soil of the UK railway network as a potential stabilisation technique of this soil using indigenous ureolytic bacteria. The soil is peat, a soft foundation soil also subject to oxidation wastage. As the peat is under existing embankments, electrokinetics (EK) is proposed as a promising technique to implement treatments. In the context of unsaturated soils the paper thus focuses on two particular aspects relevant for the implementation of treatments and the stability of this soil, namely: a) the effect of degree of saturation of the peat on the bio-electrokinetic treatment ; b) the soil water retention curve of the soil affecting flow and transport; these are relevant as we focus on understanding and modelling the implementation of treatments through electrokinetics; moreover for the peat it is of importance to understand moisture exchange in the vadose zone and control groundwater table levels (e.g. during electrokinetics) in order to prevent further oxidation. After isolation and screening of indigenous microorgansisms Bacillus licheniformis was selected for further testing. The results in terms of unconfined compressive strength, CaCO3 content, swelling and compression behaviour and water retention proved the feasibility of biocementation using this indigenous microorganism. Ongoing work is assessing the required treated soil characteristics and related required biocementation degree to solve UK rail's peat foundation problems. Upscaling of the techniques towards in situ implementation is also planned in the next stage of the research.
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14

Iamchaturapatr, Janjit, Keeratikan Piriyakul, Thanate Ketklin, Gemmina Di Emidio, and Aruz Petcherdchoo. "Sandy Soil Improvement Using MICP-Based Urease Enzymatic Acceleration Method Monitored by Real-Time System." Advances in Materials Science and Engineering 2021 (September 29, 2021): 1–12. http://dx.doi.org/10.1155/2021/6905802.

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This paper aims at monitoring the improvement of sandy soil properties with biocementation through the microbially induced calcite precipitation (MICP) method with reaction accelerations by self-developed soybean urease enzymes. In this study, the concentration of calcium ions (Ca2+ ions as CaCl2) is varied at 50, 100, 250, and 500 mM to determine an optimum shear strength. The self-developed soybean urease enzymes of 20% by volume (v/v) are used to accelerate the MICP reaction to finish within 7 days. Based on real-time monitoring bender element system and direct shear tests, the optimum Ca2+ concentration is found as 250 mM. However, a detrimental effect occurs in case of high concentration of Ca2+ as CaCl2 (500 mM) because of solution acidification from high Cl− concentration. This condition lowers CaCO3 precipitation causing the reduction of biocementation process. At equivalent shear modulus, the biocementation time of MICP-based sand with acceleration by urease enzymes is about 10 times faster than that without. Using spectrophotometer and pH meter, the ammonification rate and the solution pH of biocemented sand with acceleration by urease enzymes for 3 days are found relatively higher than those without urease enzymes for 40 days. The analyses by scanning electron microscopy (SEM) and X-ray diffraction (XRD) confirm not only the occurrence of CaCO3 binding sand particles together but also the improvement of physical strengths of sandy soil samples with the MICP-based urease enzymatic acceleration method. These results introduce an option to accelerate biocemented sandy soil improvement.
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15

Charpe, Anuja U., Madhuwanti V. Latkar, and Tapan Chakrabarti. "Biocementation: an eco-friendly approach to strengthen concrete." Proceedings of the Institution of Civil Engineers - Engineering Sustainability 172, no. 8 (December 1, 2019): 438–49. http://dx.doi.org/10.1680/jensu.18.00019.

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16

Parvizi, Sheyda, Ramin Doostmohammadi, and Foruzan Ghasemian Roodsari. "The Enhancement of Mine Waste Stability Using Biocementation." Физико-технические проблемы разработки полезных ископаемых, no. 4 (2021): 24–35. http://dx.doi.org/10.15372/ftprpi20210403.

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17

Parvizi, Sheyda, Ramin Doostmohammadi, and Foruzan Ghasemian Roodsari. "The Enhancement of Mine Waste Stability Using Biocementation." Journal of Mining Science 57, no. 4 (July 2021): 557–68. http://dx.doi.org/10.1134/s1062739121040037.

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18

Tao, Junliang, Junhong Li, Xiangrong Wang, and Ruotian Bao. "Nature-Inspired Bridge Scour Countermeasures: Streamlining and Biocementation." Journal of Testing and Evaluation 46, no. 4 (May 9, 2018): 20170517. http://dx.doi.org/10.1520/jte20170517.

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19

Dhami, Navdeep Kaur, Abhijit Mukherjee, and M. Sudhakara Reddy. "Applicability of bacterial biocementation in sustainable construction materials." Asia-Pacific Journal of Chemical Engineering 11, no. 5 (May 19, 2016): 795–802. http://dx.doi.org/10.1002/apj.2014.

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20

Pinto Vilar, Rayla, and Kaoru Ikuma. "Effects of Soil Surface Chemistry on Adsorption and Activity of Urease from a Crude Protein Extract: Implications for Biocementation Applications." Catalysts 12, no. 2 (February 18, 2022): 230. http://dx.doi.org/10.3390/catal12020230.

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In the bacterial enzyme-induced calcite precipitation (BEICP) technique for biocementation, the spatial distribution of adsorbed and catalytically active urease dictates the location where calcium carbonate precipitation and resulting cementation will occur. This study investigated the relationships between the amount of urease and total bacterial proteins adsorbed, the retained enzymatic activity of adsorbed urease, and the overall loss of activity upon adsorption, and how these relationships are influenced by changes in soil surface chemistry. In soils with hydrophobic contents higher than 20% (w/w) ratio, urease was preferentially adsorbed compared to the total amount of proteins present in the crude bacterial protein extract. Conversely, adsorption of urease onto silica sand and soil mixtures, including iron-coated sand, was much lower compared to the total proteins. Higher levels of urease activity were retained in hydrophobic-containing samples, with urease activity decreasing with lower hydrophobic content. These observations suggest that the surface manipulation of soils, such as treatments to add hydrophobicity to soil surfaces, can potentially be used to increase the activity of adsorbed urease to improve biocementation outcomes.
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21

Gao, Xuecheng, Shaokang Han, Yang Yang, Wengang Zhang, Tan Zou, and Liang Cheng. "Mechanical Behavior and Microstructural Study of Biocemented Sand under Various Treatment Methods." Geofluids 2022 (April 5, 2022): 1–11. http://dx.doi.org/10.1155/2022/6015335.

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Biocementation mainly relies on the formation of calcium carbonate to bind soil particles. This paper investigates the effectiveness of biocementation in terms of treatment methods. The previously established two-phase methods are compared with newly developed one-phase-low-pH methods on their mechanical behavior and microstructure. The one-phase-low-pH methods present a higher urease fixation rate than two-phase methods, highlighting the cost-effectiveness of this method. A modified one-phase-low-pH using CH3COOH is also compared with HCl-based one-phase-low-pH method. The results show that the morphology and size of precipitated crystals have a great influence on strength development. An optimized treatment method based on the one-phase-low-pH method is also established, which is accomplished via the injection of 5 U/ml of bacterial culture together with 2 M of cementation solution during each treatment. After four times of treatments, a total of 7% cumulative calcium carbonate content can be obtained with an unconfined compressive strength of 2.15 MPa.
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22

Martuscelli, Carolina, Célia Soares, Nelson Lima, and Aires Camões. "Potential of fungi to produce bioconcrete." RILEM Technical Letters 5 (December 30, 2020): 157–62. http://dx.doi.org/10.21809/rilemtechlett.2020.119.

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The cracks in concrete reduce their resistance capacity and allow the entry of harmful agents both for their microstructure and for the reinforcements located inside the structure. Several studies have been done to promote sustainable solutions for this problem. The microbiologically induced calcium carbonate precipitation (MICCP) is an alternative to traditionally used methods and a way to reduce the environmental impact of using more cement and polymers. Most of the biocementation studies to fill cracks or to promote bio self-healing on concrete present bacteria as the microorganisms responsible for the CaCO3 induction process. Fungi are potentially better for the biocementation process because they have more biomass and some develop filaments that can be used as microfibers on materials. Thus, the present work proposes the development of a methodology to analyse the potential use of two urease-positive fungi (Penicillium chrysogenum MUM 9743 and Neurospora crassa MUM 9208) to produce bioconcrete. The microstructure and chemical constituents of biocrystals formed due to MICCP were observed under Scanning Electron Microscopy (SEM). SEM showed fungal mycelia as bio-based fiber in bioconcrete and clusters of probable calcite crystals on and around mycelia. Both fungi were able to promote biocimentation of sand.
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23

Song, Chenpeng, Sheng Zhi, Gan Feng, and Junzhi Lin. "Enhancing potential of hydrofracturing in mylonitic coal by biocementation." Energy Science & Engineering 9, no. 4 (January 6, 2021): 565–76. http://dx.doi.org/10.1002/ese3.860.

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24

Jeong, Jin-Hoon, Yoon-Soo Jo, Chang-Seon Park, Chang-Ho Kang, and Jae-Seong So. "Biocementation of Concrete Pavements Using Microbially Induced Calcite Precipitation." Journal of Microbiology and Biotechnology 27, no. 7 (July 28, 2017): 1331–35. http://dx.doi.org/10.4014/jmb.1701.01041.

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25

Chu, Jian, Volodymyr Ivanov, Maryam Naeimi, Viktor Stabnikov, and Han-Long Liu. "Optimization of calcium-based bioclogging and biocementation of sand." Acta Geotechnica 9, no. 2 (December 11, 2013): 277–85. http://dx.doi.org/10.1007/s11440-013-0278-8.

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26

Choi, Sun-Gyu, Shifan Wu, and Jian Chu. "Biocementation for Sand Using an Eggshell as Calcium Source." Journal of Geotechnical and Geoenvironmental Engineering 142, no. 10 (October 2016): 06016010. http://dx.doi.org/10.1061/(asce)gt.1943-5606.0001534.

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27

Verba, C., A. R. Thurber, Y. Alleau, D. Koley, F. Colwell, and M. E. Torres. "Mineral changes in cement-sandstone matrices induced by biocementation." International Journal of Greenhouse Gas Control 49 (June 2016): 312–22. http://dx.doi.org/10.1016/j.ijggc.2016.03.019.

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28

Achal, Varenyam, Xiangliang Pan, Duu-Jong Lee, Deepika Kumari, and Daoyong Zhang. "Remediation of Cr(VI) from chromium slag by biocementation." Chemosphere 93, no. 7 (October 2013): 1352–58. http://dx.doi.org/10.1016/j.chemosphere.2013.08.008.

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29

Kakelar, Mahdi Maleki, Sirous Ebrahimi, and Mohammadjavad Hosseini. "Improvement in soil grouting by biocementation through injection method." Asia-Pacific Journal of Chemical Engineering 11, no. 6 (July 31, 2016): 930–38. http://dx.doi.org/10.1002/apj.2027.

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30

Cacchio, Paola, and Maddalena Del Gallo. "A Novel Approach to Isolation and Screening of Calcifying Bacteria for Biotechnological Applications." Geosciences 9, no. 11 (November 14, 2019): 479. http://dx.doi.org/10.3390/geosciences9110479.

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Bacterial calcium-carbonate precipitation (BCP) has been studied for multiple applications such as remediation, consolidation, and cementation. Isolation and screening of strong calcifying bacteria is the main task of BCP-technique. In this paper, we studied CaCO3 precipitation by different bacteria isolated from a rhizospheric soil in both solid and liquid media. It has been found, through culture-depending studies, that bacteria belonging to Actinobacteria, Gammaproteobacteria, and Alphaproteobacteria are the dominant bacteria involved in CaCO3 precipitation in this environment. Pure and mixed cultures of selected strains were applied for sand biocementation experiments. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) analyses of the biotreated samples revealed the biological nature of the cementation and the effectiveness of the biodeposition treatment by mixed cultures. X-ray diffraction (XRD) analysis confirmed that all the calcifying strains selected for sand biocementation precipitated CaCO3, mostly in the form of calcite. In this study, Biolog® EcoPlate is evaluated as a useful method for a more targeted choice of the sampling site with the purpose of obtaining interesting candidates for BCP applications. Furthermore, ImageJ software was investigated, for the first time to our knowledge, as a potential method to screen high CaCO3 producer strains.
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31

Cardoso, Rafaela, Joana Vieira, and Inês Borges. "Water Retention Curve of Biocemented Sands Using MIP Results." Applied Sciences 12, no. 20 (October 17, 2022): 10447. http://dx.doi.org/10.3390/app122010447.

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Biocementation is a soil treatment technique wherein bacteria living in soil pores promote the precipitation of calcium carbonate. One of the most recent applications of this treatment is to provide resistance against the erosion of slopes by creating a resistant cover but still allowing infiltration to avoid water runoff. For modeling infiltration, it is fundamental to know the water retention curve of the treated material. This may not be an easy task because the soils most suitable for biocementation treatment are sands, due to their large pore sizes and consequent high permeability. The water retention curves (WRCs) of such types of soil are characterized for having a very small air entry value, followed by an almost-horizontal zone, which cannot be measured by using the vapor equilibrium, most of the existing sensors, or a water dewpoint potentiometer. Data from mercury intrusion porosimetry (MIP) tests can be used as an alternative to find the WRC, and this is explored in this paper. The model for the water retention curve presented considers the deformability of the soil during the MIP test, assuming an isotropic elastic behavior. The WRC derived from the MIP tests is well-fitted to the points measured by using a water dewpoint psychrometer (only for suctions above 1 MPa) and vapor equilibrium.
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32

Kang, Bo, Fusheng Zha, Weihao Deng, Runkai Wang, Xianguo Sun, and Zhitang Lu. "Biocementation of Pyrite Tailings Using Microbially Induced Calcite Carbonate Precipitation." Molecules 27, no. 11 (June 4, 2022): 3608. http://dx.doi.org/10.3390/molecules27113608.

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Tailing sand contains a large number of heavy metals and sulfides that are prone to forming acid mine drainage (AMD), which pollutes the surrounding surface environment and groundwater resources and damages the ecological environment. Microbially induced calcium carbonate precipitation (MICP) technology can biocement heavy metals and sulfides in tailing sand and prevent pollution via source control. In this study, through an unconfined compressive strength test, permeability test, and toxic leaching test (TCLP), the curing effect of MICP was investigated in the laboratory and the effect of grouting rounds on curing was also analyzed. In addition, the curing mechanism of MICP was studied by means of Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM). The experimental results showed that MICP could induce calcium carbonate precipitation through relatively complex biochemical and physicochemical reactions to achieve the immobilization of heavy metals and sulfides and significantly reduce the impact of tailing sand on the surrounding environment.
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33

Miftah, Ahmed, Hamed Khodadadi Tirkolaei, and Huriye Bilsel. "Biocementation of Calcareous Beach Sand Using Enzymatic Calcium Carbonate Precipitation." Crystals 10, no. 10 (October 1, 2020): 888. http://dx.doi.org/10.3390/cryst10100888.

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Beach sands are composed of a variety of minerals including quartz and different carbonate minerals. Seawater in beach sand contains several ions such as sodium, magnesium, calcium, chloride, sulfate, and potassium. These variations in mineralogy and the presence of salts in beach sand may affect the treatment via enzyme-induced carbonate precipitation (EICP). In this study, set test tube experiments were conducted to evaluate the precipitation kinetics and mineral phase of the precipitates in the presence of zero, five, and ten percent seawater (v/v). The kinetics were studied by measuring electrical conductivity (EC), pH, ammonium concentration, and carbonate precipitation mass in EICP solution at different time intervals. A beach sand was also treated using EICP solution containing zero and ten percent seawater at one, two, and three cycles of treatment. Unconfined compressive strength (UCS), carbonate content, and mineralogy of the precipitates in the treated specimens were evaluated. The kinetics study showed that the rate of urea hydrolysis and the rate of precipitation for zero, five, and ten percent seawater were similar within the first 16 h of the reaction. After 16 h, it was observed that the rates dropped in the solution containing seawater, which might be attributed to the faster decay rate of urease enzyme when seawater is present. All the precipitates from the test tube experiments contained calcite and vaterite, with an increase in vaterite content by increasing the amount of seawater. The presence of ten percent seawater was found to not significantly affect the UCS, carbonate content, and mineralogy of the precipitates of the treated beach sand.
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34

Chiet, K. T. P., K. A. Kassim, K. B. Chen, U. Martula, C. S. Yah, and A. Arefnia. "Effect of Reagents Concentration on Biocementation of Tropical Residual Soil." IOP Conference Series: Materials Science and Engineering 136 (July 2016): 012030. http://dx.doi.org/10.1088/1757-899x/136/1/012030.

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35

Levett, Alan, Emma J. Gagen, Paulo M. Vasconcelos, Yitian Zhao, Anat Paz, and Gordon Southam. "Biogeochemical cycling of iron: Implications for biocementation and slope stabilisation." Science of The Total Environment 707 (March 2020): 136128. http://dx.doi.org/10.1016/j.scitotenv.2019.136128.

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36

Ivanov, Volodymyr, Viktor Stabnikov, Olena Stabnikova, and Zubair Ahmed. "Biocementation technology for construction of artificial oasis in sandy desert." Journal of King Saud University - Engineering Sciences 32, no. 8 (December 2020): 491–94. http://dx.doi.org/10.1016/j.jksues.2019.07.003.

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Lai, Han-Jiang, Ming-Juan Cui, Shi-Fan Wu, Yang Yang, and Jian Chu. "Retarding effect of concentration of cementation solution on biocementation of soil." Acta Geotechnica 16, no. 5 (February 4, 2021): 1457–72. http://dx.doi.org/10.1007/s11440-021-01149-1.

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Ping Chiet, Kenny Tiong, Khairul Anuar Kassim, and Siaw Yah Chong. "Biocementation Potential of Tropical Residue Soil Infused with Facultative Anaerobe Bacteria." Applied Mechanics and Materials 773-774 (July 2015): 1412–16. http://dx.doi.org/10.4028/www.scientific.net/amm.773-774.1412.

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Biomediated soil improvement, a promising new branch of microbial geotechnology; which involved multi disciplines has successfully attracted abundant attentions among researchers, geotechnical engineers, and other industries practitioners. Few of the researches were conducted to examine the potential implementation of this technique on tropical residue soil. However, the uncertainties outcomes and inconsistency of bio mediated soil improvement, especially on the clayed soil have made this technique remained at the laboratory stage. Therefore, this paper intended to provide better understanding of this technique by investigating the relation between the bacteria, cementation reagents, and tropical residue soil. The residual soil was mixed with facultative anaerobe bacteria, Bacillus Subtilis before it was compacted into a prefabricated PVC mould. The soil samples were treated with different treatment condition such as (1) control or untreated, (2) treated with cementation solution, (3) treated with bacteria only, and (4) treated with bacteria and Cementation reagent. A worth noting finding showed that the sample treated with bacteria and nutrient only has produced the highest increment of shear strength. This phenomenon might have been caused by the effect of the chemical reagent to the mineralogy of residue soil. The presence of the chemical reagents is believed to have weakened the shear strength of the tropical residual soil.
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39

Kandasami, Ramesh K., Renee M. Borges, and Tejas G. Murthy. "Effect of biocementation on the strength and stability of termite mounds." Environmental Geotechnics 3, no. 2 (April 2016): 99–113. http://dx.doi.org/10.1680/jenge.15.00036.

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Graddy, Charles M. R., Michael G. Gomez, Jason T. DeJong, and Douglas C. Nelson. "Native Bacterial Community Convergence in Augmented and Stimulated Ureolytic MICP Biocementation." Environmental Science & Technology 55, no. 15 (July 19, 2021): 10784–93. http://dx.doi.org/10.1021/acs.est.1c01520.

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SCHWANTES-CEZARIO, N., M. F. PORTO, G. F. B. SANDOVAL, G. F. N. NOGUEIRA, A. F. COUTO, and B. M. TORALLES. "Effects of Bacillus subtilis biocementation on the mechanical properties of mortars." Revista IBRACON de Estruturas e Materiais 12, no. 1 (February 2019): 31–38. http://dx.doi.org/10.1590/s1983-41952019000100005.

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Abstract This study aims to evaluate the influence of B. subtilis AP91 spores addition on the mechanical properties of mortars. B. subtilis strain AP91, isolated from rice leaves of the needle variety, which has an early cycle of production, was used at the concentration of 105 spores/mL in mortars with cement-to-sand ratio of 1:3 (by weight) and water-to-cement ratio (w/c) of 0.63. These spores were added in two different ways: in the mixing water and by immersion in a solution containing bacterial spores. Scanning Electron Microscope (SEM) analysis showed crystals with calcium peaks on the EDS, which possibly indicates the presence of bioprecipitated calcium carbonate. The results obtained in the mechanical analysis showed that the bioprecipitation of CaCO3 by B. subtilis strain AP91 was satisfactory, particularly when the spores were added in the mixing water, increasing the compressive strength up to 31%. Thus, it was concluded that the addition of B. subtilis AP91 spores in the mixing water of cement mortars induced biocementation, which increased the compressive strength. This bioprecipitation of calcium carbonate may very well have other advantageous consequences, such as the closure of pores and cracks in cementitious materials that could improve durability properties, although more research is still needed on this matter.
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Behzadipour, Hamed, Mohammad Siroos Pakbaz, and Gholam Reza Ghezelbash. "Effects of biocementation on strength parameters of silty and clayey sands." Bioinspired, Biomimetic and Nanobiomaterials 9, no. 1 (March 1, 2020): 24–32. http://dx.doi.org/10.1680/jbibn.19.00002.

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Rao, Sudhakar M., Reshma Sukumar, Rita Evelyne Joshua, and Nitish Venkateswarlu Mogili. "Biocementation of soft soil by carbonate precipitate and polymeric saccharide secretion." Bioinspired, Biomimetic and Nanobiomaterials 9, no. 4 (December 1, 2020): 241–51. http://dx.doi.org/10.1680/jbibn.20.00032.

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Abdel-Aleem, Heba, Tarek Dishisha, Amal Saafan, Abduallah A. AbouKhadra, and Yasser Gaber. "Biocementation of soil by calcite/aragonite precipitation usingPseudomonas azotoformansandCitrobacter freundiiderived enzymes." RSC Advances 9, no. 31 (2019): 17601–11. http://dx.doi.org/10.1039/c9ra02247c.

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Kaur, Gurvinder, Navdeep Kaur Dhami, Shweta Goyal, Abhijit Mukherjee, and M. Sudhakara Reddy. "Utilization of carbon dioxide as an alternative to urea in biocementation." Construction and Building Materials 123 (October 2016): 527–33. http://dx.doi.org/10.1016/j.conbuildmat.2016.07.036.

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Bhutange, Snigdha P., M. V. Latkar, and T. Chakrabarti. "Studies on efficacy of biocementation of cement mortar using soil extract." Journal of Cleaner Production 274 (November 2020): 122687. http://dx.doi.org/10.1016/j.jclepro.2020.122687.

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47

Liu, Lu, Hanlong Liu, Yang Xiao, Jian Chu, Peng Xiao, and Yang Wang. "Biocementation of calcareous sand using soluble calcium derived from calcareous sand." Bulletin of Engineering Geology and the Environment 77, no. 4 (August 25, 2017): 1781–91. http://dx.doi.org/10.1007/s10064-017-1106-4.

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Sidik, Waleed S., Hanifi Canakci, Ibrahim H. Kilic, and Fatih Celik. "Applicability of biocementation for organic soil and its effect on permeability." Geomechanics and Engineering 7, no. 6 (December 25, 2014): 649–63. http://dx.doi.org/10.12989/gae.2014.7.6.649.

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Chu, Jian, Volodymyr Ivanov, Viktor Stabnikov, Jia He, Bing Li, and Maryam Naemi. "Biocement: Green Building- and Energy-Saving Material." Advanced Materials Research 347-353 (October 2011): 4051–54. http://dx.doi.org/10.4028/www.scientific.net/amr.347-353.4051.

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Cement and chemical grouts have often been used for soil strengthening. However, high cost, energy consumption, and harm to environment restrict their applications. Biocement could be a new green building- material and energy-saving material. Biocement is a mixture of enzymes or microbial biomass with inorganic chemicals, which can be produced from cheap raw materials. Supply of biocementing solution to the porous soil or mixing of dry biocement with clayey soil initiate biocementation of soil due to specific enzymatic activity. Different microorganisms and enzymes can be used for production of biocement.
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Javadi, Neda, Hamed Khodadadi Tirkolaei, Nasser Hamdan, and Edward Kavazanjian. "Longevity of Raw and Lyophilized Crude Urease Extracts." Sustainable Chemistry 2, no. 2 (May 6, 2021): 325–34. http://dx.doi.org/10.3390/suschem2020018.

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The stability (longevity of activity) of three crude urease extracts was evaluated in a laboratory study as part of an effort to reduce the cost of urease for applications that do not require high purity enzyme. A low-cost, stable source of urease will greatly facilitate engineering applications of urease such as biocementation of soil. Inexpensive crude extracts of urease have been shown to be effective at hydrolyzing urea for carbonate precipitation. However, some studies have suggested that the activity of a crude extract may decrease with time, limiting the potential for its mass production for commercial applications. The stability of crude urease extracts shown to be effective for biocementation was studied. The crude extracts were obtained from jack beans via a simple extraction process, stored at room temperature and at 4 ℃, and periodically tested to evaluate their stability. To facilitate storage and transportation of the extracted enzyme, the longevity of the enzyme following freeze drying (lyophilization) to reduce the crude extract to a powder and subsequent re-hydration into an aqueous solution was evaluated. In an attempt to improve the shelf life of the lyophilized extract, dextran and sucrose were added during lyophilization. The stability of purified commercial urease following rehydration was also investigated. Results of the laboratory tests showed that the lyophilized crude extract maintained its activity during storage more effectively than either the crude extract solution or the rehydrated commercial urease. While incorporating 2% dextran (w/v) prior to lyophilization of the crude extract increased the overall enzymatic activity, it did not enhance the stability of the urease during storage.
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